CN220520474U - Culture device for detecting vibrio content in seedling water body - Google Patents
Culture device for detecting vibrio content in seedling water body Download PDFInfo
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- CN220520474U CN220520474U CN202420129376.1U CN202420129376U CN220520474U CN 220520474 U CN220520474 U CN 220520474U CN 202420129376 U CN202420129376 U CN 202420129376U CN 220520474 U CN220520474 U CN 220520474U
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- 241000607598 Vibrio Species 0.000 title claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 20
- 239000001963 growth medium Substances 0.000 claims abstract description 57
- 230000007246 mechanism Effects 0.000 claims abstract description 15
- 238000005192 partition Methods 0.000 claims abstract description 12
- 230000000149 penetrating effect Effects 0.000 claims abstract description 4
- 239000011229 interlayer Substances 0.000 claims description 13
- 238000010438 heat treatment Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 abstract description 14
- 230000008569 process Effects 0.000 abstract description 13
- 238000009360 aquaculture Methods 0.000 description 6
- 244000144974 aquaculture Species 0.000 description 6
- 206010047400 Vibrio infections Diseases 0.000 description 5
- 230000003028 elevating effect Effects 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 210000003437 trachea Anatomy 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model belongs to the technical field of biological culture, and particularly relates to a culture device for detecting the content of vibrio in a seedling water body, which comprises a box body, wherein a box door is arranged on the side wall of the box body, a bracket is arranged at the bottom of the box body, a horizontal partition plate is arranged at the lower part of the box body, the top surface of the partition plate is connected with a supporting seat, and a plurality of placing grooves are formed in the supporting seat; the bottom of the partition plate is rotationally connected with a rotating mechanism, the rotating mechanism comprises a rotating shaft, the top of the rotating shaft is fixedly connected with a supporting seat, the lower part of the rotating shaft is fixedly provided with a grooved pulley, a radial groove is arranged on the grooved pulley, one side of the grooved pulley is provided with a driving plate, the driving plate is provided with a driving post, and the grooved pulley is matched with the driving plate; a driving shaft is fixedly arranged in the driving plate in a penetrating way, and the driving shaft is connected with a motor. The culture device provided by the utility model is convenient for an experimenter to take and place the culture medium in the use process, the culture medium can be taken and placed without crossing the culture medium at the front side or placing the culture medium at the front side at one side, and adjacent culture mediums cannot be touched in the taking and placing process, so that the experimenter is facilitated in experimental operation.
Description
Technical Field
The utility model belongs to the technical field of biological culture, and particularly relates to a culture device for detecting the content of vibrio in a seedling water body.
Background
Vibrio is gram negative bacteria, widely exists in aquaculture water, and is an important pathogenic bacteria causing diseases of aquaculture fish, shrimp, shellfish and the like. Vibrio is easy to propagate in large quantity when the residual bait and feces are increased, the water quality substrate is deteriorated, and the vibriosis of the aquaculture animals is easy to burst and spread rapidly when the number of the vibrios exceeds a threshold value. Therefore, accurately counting the total amount of vibrios in aquaculture water is of great importance for healthy aquaculture. Currently, TCBS medium is widely used by farmers in aquaculture for vibrio counting.
During cultivation, the number of vibrios in a seedling water body is usually required to be detected, and the detection process is generally as follows: taking 0.1mL of water sample by using a graduated pipette, wherein the suction pipette without the graduation is used for dripping 3 drops of water sample on TCBS culture medium, placing the culture medium at a constant temperature of 35-37 ℃ for culturing for 18-24 hours (the proper prolonged culturing time which is not reached), counting yellow or green colonies with larger, round, semitransparent, smooth and slightly raised colonies, multiplying the colonies with the number of vibrios of 1 milliliter by 10, and not counting the colonies smaller than 1 millimeter.
In the practical use process, the culture medium after inoculation is usually directly placed into the incubator for culture, when the culture medium is placed in the incubator, the culture vessel at the rear side is required to be taken out, the culture vessel at the rear side needs to be stretched over the culture medium at the front side, or the culture vessel at the front side is placed aside, so that the culture vessel at the rear side can be taken out, and the adjacent culture medium can be touched in the taking and placing process, which is not beneficial to the operation of experimental staff.
Disclosure of Invention
The utility model aims to provide a culture device for detecting the content of vibrio in a seedling water body so as to solve the problems in the background technology.
In order to achieve the technical purpose, the technical scheme of the utility model comprises the following steps:
the culture device for detecting the vibrio content in the seedling water body comprises a box body, wherein a box door is arranged on the side wall of the box body, a bracket is arranged at the bottom of the box body, a horizontal partition plate is fixedly arranged at the lower part of the box body, the top surface of the partition plate is rotationally connected with a supporting seat, and a plurality of placing grooves are formed in the supporting seat;
the bottom of the partition plate is rotationally connected with a rotating mechanism, the rotating mechanism comprises a rotating shaft, the top of the rotating shaft rotates to penetrate through the partition plate and extends upwards to fixedly connect with the bottom surface of the supporting seat, the bottom of the rotating shaft rotates to be connected with the bottom wall of the box body, a sheave is fixedly arranged at the lower part of the rotating shaft, a radial groove is formed in the sheave, a driving plate is arranged on one side of the sheave, a driving post is fixedly arranged on the driving plate, and the sheave is matched with the driving plate;
the driving plate is internally and fixedly provided with a driving shaft in a penetrating way, the bottom of the driving shaft is connected with a motor, and the motor is fixed on the bottom wall of the box body.
As an improvement, the bottom of standing groove is equipped with elevating system, elevating system includes the lifter plate that the level set up, the lifter plate is located the below of culture medium, the bottom of lifter plate rotates and connects vertical guide bar, the outer wall cover of guide bar is equipped with the pressure spring, the pressure spring is located the standing groove of lifter plate below, the gag lever post is connected to the bottom of guide bar, the outer wall of supporting seat is equipped with the spacing groove, the gag lever post cooperatees with the spacing groove.
As a further improvement, the diameter of the placing groove is larger than that of the culture medium, an air bag interlayer is arranged in a gap between the inner wall of the placing groove and the outer wall of the culture medium, the air bag interlayer is fixedly connected with the inner wall of the placing groove, and an air pump is installed in the middle of the supporting seat and communicated with each air bag interlayer through an air pipe.
As a further improvement, a camera is fixedly arranged on the inner side of the top wall of the box body, the camera is electrically connected with a processor, and the processor is electrically connected with a computer.
As a further improvement, an ultraviolet lamp and a heating tube are arranged in the box body.
By adopting the technical scheme, the utility model has the beneficial effects that:
according to the culture device for detecting the vibrio content in the seedling water body, the supporting seat rotates, so that each placing groove can be rotated to the box door, and each culture medium can be conveniently taken and placed from the placing groove. The culture medium is driven to lift by the lifting mechanism, so that the culture medium is not easy to move in the rotating process of the supporting seat. The culture medium is fixed in the placing groove more firmly by arranging the air bag interlayer, so that the stability in the culture process is guaranteed.
In the in-service use process, make things convenient for the experimenter to take and place the culture medium, need not to cross the culture medium of front side or place the front side culture medium to one side just can get and put to get and put the in-process and can not touch adjacent culture medium, be favorable to experimental operation.
Drawings
FIG. 1 is a schematic view of the internal structure of the present utility model;
FIG. 2 is a schematic view of the external structure of the present utility model;
FIG. 3 is a cross-sectional view of the lift mechanism;
FIG. 4 is a schematic view of the construction of the sheaves and dials;
wherein: 1-box body, 2-box door, 3-bracket, 4-baffle, 5-supporting seat, 6-placing groove, 7-culture medium, 8-rotating shaft, 9-grooved pulley, 10-driving plate, 11-driving column, 12-driving shaft, 13-motor, 14-lifting plate, 15-guide rod, 16-pressure spring, 17-limit rod, 18-limit groove, 19-air bag interlayer, 20-air pump, 21-camera, 22-ultraviolet lamp and 23-heating pipe.
Detailed Description
The utility model will be further described with reference to the following detailed description and the accompanying drawings. Wherein the drawings are for illustrative purposes only and are shown in schematic, non-physical, and not intended to limit the present application; for the purpose of better illustrating embodiments of the utility model, certain elements of the drawings may be omitted, enlarged or reduced and do not represent the size of the actual product; it will be appreciated by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numbers in the drawings of embodiments of the utility model correspond to the same or similar components; in the description of the present utility model, it should be understood that, if there is an azimuth or positional relationship indicated by terms such as "upper", "lower", "left", "right", etc., based on the azimuth or positional relationship shown in the drawings, it is only for convenience of describing the present utility model and simplifying the description, but it is not indicated or implied that the apparatus or element referred to must have a specific azimuth, be constructed and operated in a specific azimuth, and thus terms describing the positional relationship in the drawings are merely illustrative and should not be construed as limitations of the present application, and specific meanings of the terms described above may be understood by those skilled in the art according to specific circumstances.
As shown in figures 1-4, a culture apparatus for detecting vibrio content in a seedling water body comprises a box body 1, wherein a box door 2 is arranged on the side wall of the box body 1, a bracket 3 is arranged at the bottom of the box body 1, a horizontal partition plate 4 is fixedly arranged at the lower part of the box body 1, the top surface of the partition plate 4 is rotationally connected with a supporting seat 5, a plurality of placing grooves 6 are arranged on the supporting seat 5, a culture medium 7 is placed in the placing grooves 6, the placing grooves 6 can limit the culture medium 7, and the culture medium 7 is prevented from moving.
The bottom rotation of baffle 4 is connected with slewing mechanism, and slewing mechanism includes pivot 8, and baffle 4 is passed in the rotation of pivot 8's top to upwards extend the bottom surface of fixed connection supporting seat 5, and the bottom of pivot 8 rotates the connection box 1 diapire, and pivot 8's lower part is fixed to be equipped with sheave 9, is equipped with radial groove on sheave 9, and one side of sheave 9 is equipped with driver plate 10, and the fixed plectrum 11 that is equipped with on the driver plate 10, sheave 9 and driver plate 10 cooperate. When the dial 10 rotates at a certain speed, the dial column 11 rotates along with the dial column, the dial column 11 can drive the grooved wheel 9 to rotate along with the dial column after entering the radial groove of the grooved wheel 9, and the grooved wheel 9 stops rotating after the dial column 11 leaves the radial groove, so that the dial 10 continuously rotates to drive the grooved wheel 9 to intermittently rotate, and the grooved wheel 9 drives the supporting seat 5 to intermittently rotate through the rotating shaft 8.
A driving shaft 12 is fixedly arranged in the driving plate 10 in a penetrating way, a motor 13 is connected to the bottom of the driving shaft 12, the top of the driving shaft 12 is rotatably connected with the partition plate 4, the motor 13 is fixed on the bottom wall of the box body 1, and the motor 13 enables the driving shaft 12 to rotate so as to drive the driving plate 10 to rotate.
In this embodiment, the number of radial grooves is equal to the number of placement grooves 6, and the placement grooves 6 stop at the door 2 after the sheaves 9 stop, so that each culture medium 7 can stay at the door 2, facilitating the taking and placing of the culture medium 7. Therefore, the experimenter is not required to manually adjust the position of the culture medium 7, and only needs to take out or place the culture medium 7 when the support seat 5 is rotated to a proper position, so that the taking and placing operation is convenient.
Specifically, the bottom of standing groove 6 is equipped with elevating system, elevating system includes the lifter plate 14 of level setting, lifter plate 14 can reciprocate in standing groove 6, lifter plate 14 is located the below of culture medium 7, be used for holding up culture medium 7, vertical guide bar 15 is connected in the bottom rotation of lifter plate 14, the outer wall cover of guide bar 15 is equipped with pressure spring 16, pressure spring 16 is arranged in the standing groove 6 of lifter plate 14 below, can jack up lifter plate 14 through pressure spring 16, can drive lifter plate 14 downwardly moving through pull down guide bar 15, limit lever 17 is connected to the bottom of guide bar 15, the outer wall of supporting seat 5 is equipped with spacing groove 18, limit lever 17 cooperatees with spacing groove 18, through making limit lever 17 rotate in the horizontal direction, move limit lever 17 in or shift out from spacing groove 18.
In this embodiment, after the limiting rod 17 is moved out of the limiting groove 18, under the action of the elastic force of the compression spring 16, the lifting plate 14 and the guide rod 15 move upwards, so as to push the culture medium 7 to move upwards, and the culture medium 7 is convenient to take; when the culture medium 7 is placed in the placing groove 6 for culture, the limiting rod 17 is held by a hand to pull downwards, the guide rod 15 and the lifting plate 14 move downwards, when the limiting rod 17 moves to correspond to the limiting groove 18, the limiting rod 17 is placed in the limiting groove 18 to play a limiting role, the culture medium 7 is located inside the placing groove 6, and the culture medium 7 is not easy to move in the rotating process of the supporting seat 5.
Specifically, the diameter of standing groove 6 is greater than the diameter of culture medium 7, is equipped with gasbag intermediate layer 19 in the clearance between standing groove 6 inner wall and the culture medium 7 outer wall, and gasbag intermediate layer 19 fixed connection is at the inner wall of standing groove 6, and air pump 20 is installed to the centre of supporting seat 5, and air pump 20 communicates with each gasbag intermediate layer 19 through the trachea. After the culture medium 7 is placed in the placing groove 6, the air pump 20 inflates each air bag interlayer 19 through the air pipe, so that the air bag interlayer 19 is inflated, the culture medium 7 is firmly fixed in the placing groove 6, after the culture is finished, the air pump 20 pumps air, so that the air bag interlayer 19 is compressed, and then the culture medium 7 is lifted through the lifting mechanism.
Specifically, the camera 21 is fixedly arranged on the inner side of the top wall of the box body 1, the camera 21 adopts the high-temperature-resistant camera 21, the camera 21 is electrically connected with a processor, and the processor is electrically connected with a computer and is used for receiving picture information. The position of the camera 21 corresponds to the position of the placing grooves 6, so that each placing groove 6 can rotate to the position right below the camera 21, so that clear pictures can be taken, the growth condition of colonies can be observed in real time without opening the box door 2 to take out the culture medium 7 in the culture process, the times of opening and closing the box door 2 are reduced, the infection of mixed bacteria is avoided, and the constant temperature is maintained.
Specifically, the ultraviolet lamp 22 and the heating pipe 23 are arranged in the box body 1, the ultraviolet lamp 22 is used for sterilization before the cultivation starts, the heating pipe 23 is used for heating the interior of the box body 1, and the interior of the box body 1 reaches the temperature which is most suitable for the growth of strains, wherein the ultraviolet lamp 22 and the heating pipe 23 are both in the prior art.
The culture device for detecting the content of vibrio in the seedling water body provided by the utility model is used in the following steps: the ultraviolet lamp 22 is firstly turned on to disinfect, after the disinfection is finished, the motor 13 is started to enable the supporting seat 5 to intermittently rotate, then the box door 2 is opened, the limiting rod 17 which is turned into the placing groove 6 close to the box door 2 is moved out of the limiting groove 18, the lifting plate 14 is lifted by the lifting mechanism, then the culture medium 7 inoculated with vibrio is placed on the lifting plate 14, then the culture medium 7 is moved down into the placing groove 6 by the lifting mechanism, the limiting rod 17 is moved into the limiting groove 18 to be clamped, and the placing grooves 6 can be turned into the box door 2 by the rotation of the supporting seat 5, so that the culture medium 7 can be placed into the placing groove 6 for cultivation. After the culture medium 7 is placed in the placement tank 6, the air pump 20 is started to inflate, so that the air bag interlayer 19 is inflated, and the culture medium 7 is better fixed in the placement tank 6. In the culture process, the condition of each culture medium 7 can be monitored through the camera 21, so that the growth condition of strains can be mastered in time, the times of opening the box door 2 can be reduced, the infection of mixed bacteria is avoided, and the constant temperature is maintained. After the cultivation is finished, the air pump 20 is started to suck air, so that the air bag interlayer 19 is sucked, and the lifting mechanism can conveniently drive the culture medium 7 to lift.
In the actual use process, the utility model is convenient for the experimenters to take and place the culture medium 7, the experimenters can take and place the culture medium without crossing the culture medium 7 at the front side or placing the culture medium 7 at one side, and the adjacent culture medium 7 can not be touched in the taking and placing process, thereby being beneficial to experimental operation.
The above-described embodiments of the present utility model do not limit the scope of the present utility model. Any other corresponding changes and modifications made in accordance with the technical idea of the present utility model shall be included in the scope of the claims of the present utility model.
Claims (5)
1. The culture device for detecting the vibrio content in the seedling water body comprises a box body, wherein a box door is arranged on the side wall of the box body, and a bracket is arranged at the bottom of the box body;
the bottom of the partition plate is rotationally connected with a rotating mechanism, the rotating mechanism comprises a rotating shaft, the top of the rotating shaft rotates to penetrate through the partition plate and extends upwards to fixedly connect with the bottom surface of the supporting seat, the bottom of the rotating shaft rotates to be connected with the bottom wall of the box body, a sheave is fixedly arranged at the lower part of the rotating shaft, a radial groove is formed in the sheave, a driving plate is arranged on one side of the sheave, a driving post is fixedly arranged on the driving plate, and the sheave is matched with the driving plate;
the driving plate is internally and fixedly provided with a driving shaft in a penetrating way, the bottom of the driving shaft is connected with a motor, and the motor is fixed on the bottom wall of the box body.
2. The culture device for detecting the content of vibrio in a seedling water body according to claim 1, wherein a lifting mechanism is arranged at the bottom of the placing groove and comprises a lifting plate which is horizontally arranged, the lifting plate is positioned below a culture medium, the bottom of the lifting plate is rotationally connected with a vertical guide rod, the outer wall of the guide rod is sleeved with a pressure spring, the pressure spring is positioned in the placing groove below the lifting plate, the bottom of the guide rod is connected with a limit rod, the outer wall of the supporting seat is provided with a limit groove, and the limit rod is matched with the limit groove.
3. The culture device for detecting the content of vibrio in a seedling water body according to claim 2, wherein the diameter of the placing groove is larger than that of the culture medium, an air bag interlayer is arranged in a gap between the inner wall of the placing groove and the outer wall of the culture medium, the air bag interlayer is fixedly connected to the inner wall of the placing groove, an air pump is installed in the middle of the supporting seat, and the air pump is communicated with each air bag interlayer through an air pipe.
4. The culture device for detecting the content of vibrio in a seedling water body according to claim 3, wherein a camera is fixedly arranged on the inner side of the top wall of the box body, the camera is electrically connected with a processor, and the processor is electrically connected with a computer.
5. The culture apparatus for detecting the content of Vibrio in a nursery water as claimed in any one of claims 1 to 4, wherein ultraviolet lamps and heating pipes are provided in the tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202420129376.1U CN220520474U (en) | 2024-01-18 | 2024-01-18 | Culture device for detecting vibrio content in seedling water body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202420129376.1U CN220520474U (en) | 2024-01-18 | 2024-01-18 | Culture device for detecting vibrio content in seedling water body |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220520474U true CN220520474U (en) | 2024-02-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202420129376.1U Active CN220520474U (en) | 2024-01-18 | 2024-01-18 | Culture device for detecting vibrio content in seedling water body |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220520474U (en) |
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- 2024-01-18 CN CN202420129376.1U patent/CN220520474U/en active Active
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