CN220477696U - Mouse in-situ tumor-bearing device - Google Patents
Mouse in-situ tumor-bearing device Download PDFInfo
- Publication number
- CN220477696U CN220477696U CN202321628662.4U CN202321628662U CN220477696U CN 220477696 U CN220477696 U CN 220477696U CN 202321628662 U CN202321628662 U CN 202321628662U CN 220477696 U CN220477696 U CN 220477696U
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- China
- Prior art keywords
- knob
- needle tube
- cell suspension
- mouse
- injection mechanism
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- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 25
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 16
- 238000002347 injection Methods 0.000 claims abstract description 44
- 239000007924 injection Substances 0.000 claims abstract description 44
- 239000006285 cell suspension Substances 0.000 claims abstract description 31
- 230000007246 mechanism Effects 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 13
- 241000699670 Mus sp. Species 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 206010027476 Metastases Diseases 0.000 description 2
- 230000009401 metastasis Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 230000002861 ventricular Effects 0.000 description 1
Landscapes
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
The utility model belongs to the technical field of mouse in-situ tumor-bearing, and in particular relates to a mouse in-situ tumor-bearing device, which comprises: a needle tube, the interior of which is hollow; an injection needle sleeved at one end of the needle tube; wherein a quantitative injection mechanism is arranged in the syringe barrel; the quantitative injection mechanism is suitable for quantitatively injecting the cell suspension into the body of the mouse through the needle tube after the cell suspension is extracted from the interior of the needle tube; according to the utility model, the quantitative injection mechanism is arranged, after the full cell suspension is extracted, the knob is rotated, the knob is displaced for a distance after rotating for one circle, the knob is pressed, a certain amount of cell suspension is injected into the mouse body, the quantitative injection mechanism is repeated for a plurality of times, and as the rotating shaft is connected with the threaded channel and the screw pitch is fixed, the cell suspension amount injected each time is fixed.
Description
Technical Field
The utility model belongs to the technical field of in-situ tumor bearing of mice, and particularly relates to an in-situ tumor bearing device of mice.
Background
In China, about 7710 patients die from cancer every day, and about 6 patients die from cancer every minute, so that research on cancer clearly becomes a serious issue in biomedicine, and animal models play a vital role in tumorigenesis and development and drug development.
CDTX mainly includes subcutaneous tumor-bearing, various metastasis model tumor-bearing (tail vein, left ventricular injection), in situ tumor-bearing, and the like. The in-situ tumor-bearing model is used for screening and detecting medicines for inhibiting tumor growth and tumor metastasis because tumor cells are transplanted to corresponding organs of mice and tumors are easy to metastasize (such as lung cancer, breast cancer and the like).
In the in-situ tumor-bearing experiment process, the lung of the experimental mouse is small and thin, and the experimental mouse is continuously fluttered, so that the injection difficulty is improved, and the direct vision needs to be cut open and the needle is obliquely inserted. For a large number of tumors, if the cell suspension amount sucked by the injector at each time just meets the requirement of using one mouse, the cell suspension is sucked again, time and labor are wasted, and if the injector sucks more cell suspension at one time, the injection end of the injector is overlong, and the direction and the injection dosage are not easy to control; in the process of establishing an in-situ tumor-bearing model, the tumor cells with the same dose are required to be injected, the same positions such as the back, the abdomen, the limbs and the like are injected, the same depth is inserted, the consistency of experimental conditions is maintained, and a single variable is studied, so that the accurate injection positioning has important significance. Therefore, it is necessary to reduce the negative effects of repeated suction of the cell suspension and the decrease in success rate of the tumor bearing thus obtained, while ensuring the accuracy of the injected cell suspension; it is desirable to design a mouse in situ tumor bearing device such that the cell suspension dose is the same for each injection.
Disclosure of Invention
The utility model aims to provide a mouse in-situ tumor-bearing device, which aims to solve the technical problem that the cell suspension volume injected each time is difficult to control uniformly.
In order to solve the technical problems, the utility model provides a mouse in-situ tumor-bearing device, which comprises: a needle tube, the interior of which is hollow; an injection needle sleeved at one end of the needle tube; wherein a quantitative injection mechanism is arranged in the syringe barrel; the quantitative injection mechanism is suitable for quantitatively injecting the cell suspension into the mouse body through the injection needle after the cell suspension is extracted from the needle tube.
Further, the quantitative injection mechanism includes: and the piston is connected with the inner wall of the needle tube in a sliding way.
Further, one end of the piston is closed; and the other end of the piston is provided with a threaded passage along the axial direction.
Further, the quantitative injection mechanism further comprises: the knob is sleeved outside the other end of the needle tube and is in sliding connection with the needle tube; wherein the knob is hollow; and the center of the knob is provided with a rotating shaft, and the outer side of the knob is provided with threads.
Further, the rotating shaft is in threaded connection with a threaded channel inside the piston.
Further, an auxiliary positioning block is arranged on the outer side of the injection needle; one side of the auxiliary positioning block is connected with one end of the needle tube barrel.
The quantitative injection mechanism is arranged, after the cell suspension is extracted, the knob is rotated, the knob is displaced for a distance after rotating for one circle, the knob is pressed, a certain amount of the cell suspension is injected into the mouse body, the quantitative injection mechanism is repeated for a plurality of times, and the quantity of the cell suspension injected each time is fixed due to the fact that the rotating shaft is connected with the threaded channel and the thread pitch is fixed.
Additional features and advantages of the utility model will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the utility model. The objectives and other advantages of the utility model will be realized and attained by the structure particularly pointed out in the written description and drawings.
In order to make the above objects, features and advantages of the present utility model more comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of the overall front perspective of the device of the present utility model;
FIG. 2 is a schematic elevational cross-sectional view of the apparatus of the present utility model;
FIG. 3a is a schematic view showing the state of the quantitative injection mechanism of the present utility model before the cell suspension is extracted;
FIG. 3b is a schematic view showing the state of the quantitative injection mechanism of the present utility model after the cell suspension is extracted;
FIG. 3c is a schematic view showing a state of the quantitative injection mechanism according to the present utility model after one turn of the knob;
FIG. 3d is a schematic view of the quantitative injection mechanism of the present utility model after the knob is pressed;
fig. 4 is a schematic view of the operational state of the quantitative injection mechanism of the present utility model.
In the figure:
1. a needle tube;
2. an auxiliary positioning block;
3. an injection needle;
4. a quantitative injection mechanism; 40. a knob; 41. a rotating shaft; 42. and (3) a piston.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The embodiment provides a mouse in-situ tumor-bearing device, which comprises: a needle cylinder 1, the interior of which is hollow; an injection needle 3 sleeved at one end of the needle tube 1; wherein the interior of the syringe barrel 1 is provided with a dosing mechanism 4.
In the present embodiment, the quantitative injection mechanism 4 is adapted to inject a cell suspension into a mouse body quantitatively through the injection needle 3 after the cell suspension is withdrawn from the inside of the needle tube 1.
In this embodiment, the quantitative injection mechanism 4 includes: a piston 42 slidably connected to the inner wall of the needle tube 1.
In this embodiment, the injection needle 3 is placed in the device containing the cell suspension and the piston 42 is moved to withdraw the cell suspension into the syringe barrel 1.
In this embodiment, one end of the piston 42 is closed; and the other end of the piston 42 is provided with a threaded passage along the axial direction.
In this embodiment, the quantitative injection mechanism 4 further includes: a knob 40 sleeved outside the other end of the needle tube barrel 1 and connected with the needle tube barrel 1 in a sliding way; wherein the knob 40 is hollow; and the knob 40 is provided with a rotation shaft 41 at the center and a screw thread at the outer side thereof.
In this embodiment, the shaft 41 is screwed with a threaded passage inside the piston 42.
In this embodiment, since the rotating shaft 41 is connected with the threaded channel, the knob 40 is tightly attached to the syringe barrel 1, the knob 40 is manually rotated, the piston 42 is driven by the rotating shaft 41 to move inside the syringe barrel 1, and cell suspension is extracted (as shown in fig. 3a and 3 b), when the rotating shaft 41 completely enters the threaded channel, the cell suspension in the syringe barrel 1 is extracted, at this time, the knob 40 is reversely rotated, because friction exists between the piston 42 and the inner wall of the syringe barrel 1, and at this time, an operator does not apply force to the knob 40 to attach the knob 40 to the syringe barrel, the knob 40 is reversely rotated for one turn, the knob 40 is moved away from the syringe barrel 1 (as shown in fig. 3 c), at this time, the knob 40 is pressed to attach the knob 40 again to the syringe barrel 1, the piston 42 is pushed by the rotating shaft 41 to move a distance in the syringe barrel 1, and a certain amount of cell suspension is injected into a mouse (as shown in fig. 3 d), after the injection is completed, the knob 40 is reversely rotated a plurality of times, and the distance of the knob 40 is reversely rotated each time is fixed after the knob 40 is pressed for a turn, and the distance of the movement of the knob 40 is fixed after the knob is pressed.
In the embodiment, an auxiliary positioning block 4 is arranged on the outer side of the injection needle 3; wherein one side of the auxiliary positioning block 4 is connected with one end of the needle tube 1.
In the present embodiment, the auxiliary positioning block 4 functions to perform positioning when the mice are injected.
In summary, the quantitative injection mechanism is provided, after the cell suspension is extracted, the knob is rotated, the knob is displaced for a distance after rotating for one circle, the knob is pressed, a certain amount of cell suspension is injected into the mouse body, and the quantitative injection mechanism is repeated for a plurality of times, and the quantity of the cell suspension injected each time is fixed due to the fact that the rotating shaft is connected with the threaded channel and the thread pitch is fixed.
In the description of embodiments of the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
With the above-described preferred embodiments according to the present utility model as an illustration, the above-described descriptions can be used by persons skilled in the relevant art to make various changes and modifications without departing from the scope of the technical idea of the present utility model. The technical scope of the present utility model is not limited to the description, but must be determined according to the scope of claims.
Claims (2)
1. A mouse in situ tumor bearing device, comprising:
a needle tube, the interior of which is hollow; and
an injection needle sleeved at one end of the needle tube; wherein the method comprises the steps of
A quantitative injection mechanism is arranged in the needle tube barrel;
the quantitative injection mechanism is suitable for quantitatively injecting the cell suspension into a mouse body through an injection needle after the cell suspension is extracted from the interior of the needle tube barrel;
the quantitative injection mechanism comprises:
a piston which is connected with the inner wall of the needle tube in a sliding way;
one end of the piston is closed; and
the other end of the piston is provided with a threaded channel along the axial direction;
the quantitative injection mechanism further comprises:
the knob is sleeved outside the other end of the needle tube and is in sliding connection with the needle tube; wherein the method comprises the steps of
The knob is hollow; and
the center of the knob is provided with a rotating shaft, and the outer side of the knob is provided with threads;
the rotating shaft is in threaded connection with a threaded channel in the piston.
2. A mouse in situ tumor bearing device as in claim 1, wherein,
an auxiliary positioning block is arranged on the outer side of the injection needle; wherein the method comprises the steps of
One side of the auxiliary positioning block is connected with one end of the needle tube barrel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321628662.4U CN220477696U (en) | 2023-06-26 | 2023-06-26 | Mouse in-situ tumor-bearing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321628662.4U CN220477696U (en) | 2023-06-26 | 2023-06-26 | Mouse in-situ tumor-bearing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220477696U true CN220477696U (en) | 2024-02-13 |
Family
ID=89830386
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321628662.4U Active CN220477696U (en) | 2023-06-26 | 2023-06-26 | Mouse in-situ tumor-bearing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220477696U (en) |
-
2023
- 2023-06-26 CN CN202321628662.4U patent/CN220477696U/en active Active
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