CN220450187U

CN220450187U - Inoculating needle for detecting intestinal microbial colony

Info

Publication number: CN220450187U
Application number: CN202321731982.2U
Authority: CN
Inventors: 吴浩; 黄煦腾; 黄文�
Original assignee: First Affiliated Hospital of Naval Military Medical University of PLA
Current assignee: First Affiliated Hospital of Naval Military Medical University of PLA
Priority date: 2023-07-04
Filing date: 2023-07-04
Publication date: 2024-02-06
Anticipated expiration: 2033-07-04

Abstract

The utility model belongs to the technical field of microbial cultivation, and discloses an inoculating needle for detecting intestinal microbial colony, which comprises a barrel, wherein a liquid outlet is formed in the bottom of the barrel, a needle rod is arranged in the barrel, the lower end of the needle rod penetrates through the liquid outlet to extend out of the barrel, the diameter of the liquid outlet is larger than the outer diameter of the needle rod, an annular boss close to the liquid outlet is arranged on the inner bottom wall of the barrel, a compression ring matched with the annular boss is arranged on the needle rod, a press-rotating type cover assembly is arranged at the top of the barrel, a stop rod and an elastic assembly are arranged on the inner top wall of the press-rotating type cover assembly, a supporting table is arranged at the top of the needle rod, the lower end of the elastic assembly abuts against the supporting table, the supporting table is in sliding connection with the barrel, and the distance from the top of the supporting table to the inner top wall of the press-rotating type cover assembly is equal to the length of the stop rod in a sealing state. The utility model prevents the liquid in the cylinder from scattering due to the compression of the needle rod when the inoculating needle is not in use, thereby avoiding waste and environmental pollution; the angle of the ring strip can be adjusted so as to be suitable for the habit of holding the cylinder without operators, and the cylinder is prevented from sliding down in the operation process.

Description

Inoculating needle for detecting intestinal microbial colony

Technical Field

The utility model relates to the technical field of microbial cultivation, in particular to an inoculating needle for detecting intestinal microbial colonies.

Background

Microbial sample processing is widely used for colony inoculation, culture, separation and detection, and is an important work in the aspects of medical health, inspection and quarantine, disease control, food safety and the like. Inoculating needles are an essential component in microbial sample processing systems. However, the conventional inoculating needle has poor use convenience, usually the inoculating needle is used for scribing on a culture medium after dipping liquid, the operation is complex, the repeated operation labor capacity is large, and the inoculating needle is not suitable for mass inoculation. If the needle tube is used for storing liquid, the liquid is released outwards in a piston extruding mode, misoperation exists, and when the needle tube is stored or not inoculated, the liquid is easy to scatter once touching the pressing handle at the tail end of the piston rod, waste is caused, and the environment is possibly polluted.

In order to solve the technical problems, china patent (patent publication No. CN 214571919U) discloses a lower seal head of an inoculating needle cylinder body for detecting intestinal microbial colony, a compression ring matched with an annular boss at the inner side of the lower seal head is arranged on a needle rod, an elastic piece is arranged between the upper end of the needle rod and the upper seal head of the cylinder body, and the compression ring and the boss on the needle rod are kept to be compressed by the elastic acting force of the elastic piece under normal state so as to keep tightness; the middle part of the cylinder body is symmetrically provided with an inward concave extrusion part; the diameter of the through hole on the lower seal head for penetrating the needle bar is larger than the outer diameter of the needle bar. When the device is used, the needle bar is only required to be pressed on the surface of the culture medium or the side wall of the culture medium with a little force, the extrusion part is extruded with a little force, so that the liquid in the cylinder body can be released, and when the device is used for batch inoculation, the liquid is not required to be dipped frequently, the efficiency is high, and the labor capacity is small.

According to the technical scheme, although the technical problem that liquid is not required to be dipped frequently in batch inoculation is solved, when the device is stored or not inoculated, the liquid in the cylinder body can be scattered due to the fact that the device falls vertically or the needle rod is pressed, waste is caused, and even environmental pollution can be caused.

Disclosure of Invention

The utility model aims to provide an inoculating needle for detecting intestinal microbial colony, which aims to solve the technical problem that in the prior art, the inoculating needle drops carelessly when not in use, and liquid in a cylinder body is scattered due to the compression of a needle rod.

In order to achieve the above object, the present utility model provides the following technical solutions:

the inoculating needle for detecting intestinal microbial colony comprises a barrel, wherein a liquid outlet is formed in the bottom of the barrel, a needle rod is arranged in the barrel, the lower end of the needle rod penetrates through the liquid outlet and extends out of the barrel, the liquid outlet diameter is larger than the outer diameter of the needle rod, an annular boss close to the liquid outlet is arranged on the inner bottom wall of the barrel, and a compression ring matched with the annular boss is arranged on the needle rod; the top sealing connection of barrel has pressure to revolve the lid subassembly, pressure is equipped with pin and elastic component to revolve the interior roof of lid subassembly, the top of needle bar is equipped with the brace table, the brace table is the eccentric circle of barrel, the lower extreme of elastic component supports the brace table, brace table and barrel inner wall sliding connection, under the sealed state, the brace table top is equal to the length of baffle rod to pressure the interior roof of lid subassembly.

The principle of the technical proposal is as follows: when detecting microbial colonies, an operator firstly stores strain suspension in the cylinder, presses the pressing-rotating type cover assembly to rotate clockwise so as to enable the strain suspension to be in sealing connection with the cylinder, does not apply pressure to rotate the pressing-rotating type cover assembly anticlockwise, enables the stop lever to rotate to one end far away from the supporting table, and releases the limiting effect on the up-and-down movement of the supporting table; the needle bar is slightly pressed on the surface of the culture medium or the side wall of the culture medium by holding the cylinder body with a hand, the needle bar which moves upwards drives the compression ring to move, so that the compression ring is separated from the boss, a gap is formed between the compression ring and the boss, the diameter of the liquid outlet is larger than that of the needle bar, and under the dual functions of gravity and the flow guide of the needle bar, liquid in the cylinder body flows down along the outer circumferential surface of the needle bar through the liquid outlet, so that the uniformity of the flowing liquid is better; in the state of applying pressure, the elastic acting force of the elastic component can keep the compression ring and the boss to be compressed, so that the liquid in the cylinder is prevented from flowing out, and the continuous operation of mass inoculation is facilitated; when the operation is needed to be suspended, the anti-clockwise rotation pressing-rotating cover assembly is rotated anticlockwise under the state of no pressure, so that the stop lever is positioned right above the supporting table, the limiting effect is achieved on the supporting table, the inoculating needle can be effectively prevented from being carelessly dropped, liquid in the cylinder body is scattered due to the fact that the needle rod is pressed, and waste and environmental pollution are caused.

Further, press and revolve formula lid subassembly and include enclosing cover and inner cup, inner cup and barrel threaded connection, the inner cup cover is equipped with the bulge loop, the enclosing cover inside wall is equipped with the gliding recess of confession bulge loop, the inner cup bottom outside is equipped with the pressure and revolves the platform perpendicularly, the pressure is revolved and is equipped with a plurality of pressures on the platform and revolves the draw-in groove, the enclosing cover bottom is equipped with the pressure that supplies the pressure to revolve platform pivoted and revolves the groove, the pressure is revolved the interior roof in groove and is equipped with a plurality of pressures with pressure and revolves draw-in groove looks adaptation revolves the draw-in table, the interior roof of enclosing cover is all located to pin and elastic component.

When pressure is not applied, the pressing and rotating clamping table and the pressing and rotating clamping groove cannot be clamped, and the pressing and rotating table rotates in the pressing and rotating groove so that the outer cover and the inner cover relatively idle, and therefore the position of the stop lever is adjusted. The arrangement of the convex ring and the groove can prevent the inner cover from separating from the outer cover.

Further, the inner top wall of the outer cover is provided with an annular baffle, the inner cover is in sliding connection with the annular baffle, the inner top wall of the outer cover between the annular baffle and the inner side wall of the outer cover is provided with a first elastic sealing ring, the inner top wall of the inner cover is provided with a second elastic sealing ring, the second elastic sealing ring is located right above the top of the cylinder body, and the second elastic sealing ring is matched with the top of the cylinder body in size.

Through the setting of first elastic sealing washer and second elastic sealing washer, can ensure the leakproofness between pressure spiral lid subassembly and the barrel.

Further, the elastic component includes spring, first bracing piece and second bracing piece, spring and first bracing piece all with the outer lid inner top wall fixed connection, first bracing piece and second bracing piece sliding connection, the spring housing is located on first bracing piece and the second bracing piece, the side of brace table is equipped with the horizontal pole, the barrel inside wall is equipped with the sliding tray that supplies the horizontal pole to slide, the horizontal pole is square.

The square cross rod can support and fix the supporting table and can ensure that the supporting table cannot incline when moving up and down; and the arrangement of the first support rod and the second support rod can prevent the spring from being stressed and distorted.

Further, the middle part of the cylinder body is symmetrically provided with an inwards concave extrusion part, the extrusion part is provided with a ring strip, the extrusion part is provided with a ball groove for the ring strip to move, and the lower end of the needle rod is a tip.

Through the arrangement of the finger ring strips, the problem that the solution in the cylinder is exposed due to the fact that the inoculating needle drops carelessly in the operation process can be prevented, and waste is caused due to the fact that the liquid in the cylinder is polluted.

In summary, the utility model has the following beneficial effects:

1. the device realizes the limit function of the stop lever with the adjustable position on the needle rod of the inoculating needle filled with the strain suspension, when the inoculating needle needs to be suspended in the middle of mass inoculation of strains, the outer cover is made to idle relative to the inner cover by rotating the outer cover, the stop lever on the inner top wall of the outer cover is adjusted to be propped against the supporting table, and the needle rod can be prevented from moving upwards even if being pressed, so that the liquid in the cylinder body is prevented from scattering due to the pressing of the needle rod when the inoculating needle is not used; avoiding polluting the strain suspension in the cylinder body and causing waste and avoiding the scattered strain suspension from polluting the environment.

2. The angle of the ring strip can be adjusted so as to be suitable for the habit of holding the cylinder without operators, and the cylinder is prevented from sliding down in the operation process.

Drawings

FIG. 1 is a schematic diagram showing the structure of an inoculating needle for detecting intestinal microbial colonies;

FIG. 2 is a schematic view showing the structure of the cover of the inoculating needle for detecting intestinal microbial colony;

FIG. 3 is a schematic view of an inner cap of an inoculating needle for detecting colonies of intestinal microorganisms according to the present utility model;

the names of the corresponding marks in the drawings are:

1. a cylinder; 2. a liquid outlet; 3. a needle bar; 4. an annular boss; 5. a compression ring; 6. a press-and-turn cap assembly; 7. a stop lever; 8. an elastic component; 9. a support table; 10. an outer cover; 11. an inner cover; 12. a convex ring; 13. a groove; 14. a pressing and rotating table; 15. pressing and rotating the clamping groove; 16. a pressing and rotating groove; 17. a pressing and rotating clamping table; 18. an annular baffle; 19. a first elastic sealing ring; 20. a second elastic sealing ring; 21. a spring; 22. a first support bar; 23. a second support bar; 24. a cross bar; 25. a sliding groove; 26. an extrusion part; 27. a finger ring strip; 28. ball grooves.

Detailed Description

The utility model is described in further detail below with reference to the attached drawings and embodiments:

as shown in fig. 1 to 3, an inoculating needle for detecting intestinal microbial colony comprises a barrel 1, wherein a liquid outlet 2 is formed in the bottom of the barrel 1, a needle rod 3 is arranged in the barrel 1, the lower end of the needle rod 3 is a tip, the lower end of the needle rod 3 penetrates through the liquid outlet 2 to extend out of the barrel 1, the diameter of the liquid outlet 2 is larger than the outer diameter of the needle rod 3, an annular boss close to the liquid outlet 2 is arranged on the inner bottom wall of the barrel 1, and a compression ring 5 matched with the annular boss is arranged on the needle rod 3; the top of needle bar 3 is equipped with supporting bench 9, and supporting bench 9 is the eccentric circle of barrel 1, and supporting bench 9 side is equipped with three horizontal pole 24, and wherein two symmetries are located supporting bench 9's front and back side, and another side that is close to barrel 1 inside wall of locating supporting bench 9, and horizontal pole 24 is square, and barrel 1 inside wall is equipped with the sliding groove 25 that supplies horizontal pole 24 to slide. The middle part of the cylinder body 1 is symmetrically provided with an inward concave extrusion part 26, the extrusion part 26 is provided with a ring strip 27, and the extrusion part 26 is provided with a ball groove 28 for the ring strip 27 to move. The top sealing connection of barrel 1 has pressure to revolve formula lid subassembly 6, and the interior roof of pressure to revolve formula lid subassembly 6 is equipped with pin 7 and elastic component 8, and the lower extreme of elastic component 8 supports supporting bench 9, and under the sealed state, the distance of supporting bench 9 top to pressure to revolve formula lid subassembly 6 interior roof equals the length of pin 7.

The press-rotating type cover assembly 6 comprises an outer cover 10 and an inner cover 11, wherein the inner cover 11 is in threaded connection with the barrel body 1, a convex ring 12 is sleeved on the inner cover 11, a groove 13 for the convex ring 12 to slide is formed in the inner side wall of the outer cover 10, a press-rotating table 14 is vertically arranged on the outer side of the bottom end of the inner cover 11, a plurality of press-rotating clamping grooves 15 are formed in the press-rotating table 14 at equal intervals, a press-rotating groove 16 for the press-rotating table 14 to rotate is formed in the bottom of the outer cover 10, a plurality of press-rotating clamping tables 17 matched with the press-rotating clamping grooves 15 are formed in the inner top wall of the press-rotating groove 16, and a stop lever 7 and an elastic assembly 8 are arranged on the inner top wall of the outer cover 10. The inner top wall of the outer cover 10 is provided with an annular baffle, the inner cover 11 is in sliding connection with the annular baffle, the inner top wall of the outer cover 10 between the annular baffle and the inner side wall of the outer cover 10 is provided with a first elastic sealing ring 19, the inner top wall of the inner cover 11 is provided with a second elastic sealing ring 20, the second elastic sealing ring 20 is positioned right above the top of the cylinder 1, and the second elastic sealing ring 20 is matched with the top of the cylinder 1 in size.

The elastic component 8 includes spring 21, first bracing piece 22 and second bracing piece 23, spring 21 and first bracing piece 22 all with the interior roof fixed connection of enclosing cover 10, first bracing piece 22 and second bracing piece 23 sliding connection, spring 21 cover is located on first bracing piece 22 and the second bracing piece 23.

The specific implementation process is as follows:

when detecting microbial colonies, an operator stores strain suspension in the cylinder 1, presses the press-rotation type cover assembly 6 to rotate clockwise, so that the strain suspension is in sealing connection with the cylinder 1, when the press-rotation type cover assembly 6 rotates anticlockwise without applying pressure, the press-rotation clamping table 17 cannot be clamped with the press-rotation clamping groove 15, and the press-rotation table 14 rotates in the press-rotation groove 16, so that the outer cover 10 and the inner cover 11 idle relatively, and the position of the stop lever 7 is adjusted. The stop lever 7 is rotated to one end far away from the supporting table 9, so that the limiting effect on the up-and-down movement of the supporting table 9 can be relieved; one finger of an operator passes through the finger ring strip 27, and according to the personal holding habit, the holding angle can be adjusted through the ball groove 28, so that the inoculating needle can be prevented from falling off carelessly in the operation process; the needle bar 3 is slightly pressed on the surface of the culture medium or the side wall of the culture medium forcefully, the needle bar 3 which moves upwards drives the compression ring 5 to move, so that the compression ring 5 is separated from the boss, a gap is formed between the compression ring 5 and the boss, the diameter of the liquid outlet 2 is larger than that of the needle bar 3, liquid in the cylinder 1 flows down along the outer circumferential surface of the needle bar 3 through the extrusion of the extrusion part 26 and the flow guide of the needle bar 3, so that the uniformity of the flowing liquid is better, and in a state that the pressure is not applied to the needle bar 3, the elastic acting force of the elastic component 8 can keep the compression ring 5 to be tightly pressed with the boss, the liquid in the cylinder 1 is prevented from flowing out, and the continuous operation of mass inoculation is facilitated.

In the continuous operation process, when an emergency is encountered, the operation is usually needed to be suspended halfway, at this time, the outer cover 10 can idle relative to the inner cover 11 by rotating the pressing-rotating cover assembly 6 anticlockwise under the state of no pressure, and the stop lever 7 on the inner top wall of the outer cover 10 is regulated to be propped against the supporting table 9, so that the needle bar 3 can be prevented from moving upwards even if the needle bar 3 is pressed, and the liquid in the cylinder 1 is prevented from scattering due to the pressing of the needle bar 3 when the inoculating needle is not used; avoiding polluting the strain suspension in the cylinder body 1, causing waste and avoiding the scattered strain suspension polluting the environment.

The foregoing is merely exemplary embodiments of the present utility model, and detailed technical solutions or features that are well known in the art have not been described in detail herein. It should be noted that, for those skilled in the art, several variations and modifications can be made without departing from the technical solution of the present utility model, and these should also be regarded as the protection scope of the present utility model, which does not affect the effect of the implementation of the present utility model and the practical applicability of the patent. The protection scope of the present application shall be subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.

Claims

1. The utility model provides an intestinal microorganism colony detects and uses inoculation needle, includes barrel (1), barrel (1) bottom is equipped with liquid outlet (2), be equipped with needle bar (3) in barrel (1), needle bar (3) lower extreme passes liquid outlet (2) and extends to outside barrel (1), liquid outlet (2) diameter is greater than needle bar (3) external diameter, the interior bottom of barrel (1) is equipped with the annular boss that is close to liquid outlet (2), needle bar (3) cover is equipped with clamping ring (5) with annular boss looks adaptation; the needle bar is characterized in that the top of the cylinder body (1) is connected with a pressing and rotating type cover assembly (6) in a sealing mode, a stop lever (7) and an elastic assembly (8) are arranged on the inner top wall of the pressing and rotating type cover assembly (6), a supporting table (9) is arranged at the top of the needle bar (3), the supporting table (9) is an eccentric circle of the cylinder body (1), the lower end of the elastic assembly (8) is abutted to the supporting table (9), the supporting table (9) is connected with the inner wall of the cylinder body (1) in a sliding mode, and in a sealing mode, the distance from the top of the supporting table (9) to the inner top wall of the pressing and rotating type cover assembly (6) is equal to the length of the stop lever (7).

2. The inoculating needle for detecting intestinal microbial colonies according to claim 1, wherein the pressure rotary cover assembly (6) comprises an outer cover (10) and an inner cover (11), the inner cover (11) is in threaded connection with the barrel (1), a convex ring (12) is sleeved on the inner cover (11), a groove (13) for the convex ring (12) to slide is formed in the inner side wall of the outer cover (10), a pressure rotary table (14) is vertically arranged on the outer side of the bottom end of the inner cover (11), a plurality of pressure rotary clamping grooves (15) are formed in the pressure rotary table (14) at equal intervals, a pressure rotary groove (16) for the pressure rotary table (14) to rotate is formed in the bottom of the outer cover (10), a plurality of pressure rotary clamping tables (17) matched with the pressure rotary clamping grooves (15) are formed in the inner top wall of the pressure rotary groove (16), and the stop lever (7) and the elastic assembly (8) are both arranged on the inner top wall of the outer cover (10).

3. The inoculating needle for detecting intestinal microbial colony according to claim 2, wherein an annular baffle is arranged on the inner top wall of the outer cover (10), the inner cover (11) is slidably connected with the annular baffle, a first elastic sealing ring (19) is arranged on the inner top wall of the outer cover (10) and positioned between the annular baffle and the inner side wall of the outer cover (10), a second elastic sealing ring (20) is arranged on the inner top wall of the inner cover (11), the second elastic sealing ring (20) is positioned right above the top of the barrel (1), and the second elastic sealing ring (20) is matched with the top of the barrel (1) in size.

4. An inoculating needle for detecting intestinal microbial colonies according to claim 2 or 3, characterized in that the elastic component (8) comprises a spring (21), a first supporting rod (22) and a second supporting rod (23), the spring (21) and the first supporting rod (22) are fixedly connected with the inner top wall of the outer cover (10), the first supporting rod (22) is slidably connected with the second supporting rod (23), the spring (21) is sleeved on the first supporting rod (22) and the second supporting rod (23), a cross rod (24) is arranged on the side face of the supporting table (9), a sliding groove (25) for the cross rod (24) to slide is formed in the inner side wall of the barrel (1), and the cross rod (24) is square.

5. The inoculating needle for detecting intestinal microbial colony according to claim 4, wherein the middle part of the barrel body (1) is symmetrically provided with inwards concave extrusion parts (26), the extrusion parts (26) are provided with finger ring strips (27), the extrusion parts (26) are provided with ball grooves (28) for the finger ring strips (27) to move, and the lower end of the needle rod (3) is a tip.