CN220420364U

CN220420364U - Magnetic porous PCR plate

Info

Publication number: CN220420364U
Application number: CN202321990616.9U
Authority: CN
Inventors: 阳承利
Original assignee: Suzhou Knowledge & Benefit Sphere Tech Co ltd
Current assignee: Suzhou Knowledge & Benefit Sphere Tech Co ltd
Priority date: 2023-07-27
Filing date: 2023-07-27
Publication date: 2024-01-30
Anticipated expiration: 2033-07-27

Abstract

The utility model discloses a magnetic porous PCR plate, which comprises a PCR plate body, a base, limit posts and magnets, wherein the base is provided with a plurality of positioning grooves for embedding and supporting the bottom end of a PCR tube, the base is provided with at least one magnet at the side of each positioning groove, the cross section width of the magnet is gradually reduced from bottom to top, the upper part of the magnet is higher than the upper end face of the cone part of the PCR tube, and the limit posts are arranged at the peripheral side of the upper end of the base and used for transversely positioning the PCR plate body. According to the utility model, the shape of the magnet is matched with the shape of the cone part of the PCR tube, so that the gap between the magnet and the cone part of the PCR tube is reduced, and the upper part of the magnet is positioned above the cone part of the PCR tube, so that the adsorption range is enlarged, and the magnetic attraction effect and the magnetic attraction efficiency are improved.

Description

Magnetic porous PCR plate

Technical Field

The utility model relates to the technical field of magnetic microsphere separation devices, in particular to a magnetic porous PCR plate.

Background

The magnetic microsphere has magnetism, can be quickly gathered after being adsorbed by a magnet, and is separated from other substances, thereby achieving the purpose of separation and purification, and being widely applied to separating proteins, antibodies and the like. For single PCR tubes, one or more combined magnetic racks are generally used for positioning and then magnetically attracting the PCR tubes, but for PCR plates (such as 96 holes or more), the magnetic racks matched with the PCR plates are needed because the PCR plates have more PCR tubes arranged in a matrix shape.

In the existing magnetic frame for the PCR plate or the combined structure of the magnetic frame and the PCR plate, when a columnar magnet is used, due to the arrangement of magnetic poles and the distribution of magnetic flux, a magnetic field is mainly concentrated at two ends of the columnar magnet, so that the magnetic field at the position close to the magnetic poles is larger and more magnetic microspheres are adsorbed, the adsorption of other positions is less, and the magnetic microspheres are unevenly distributed after being adsorbed; the application number is 202223205956.4's magnetic frame and nucleic acid extraction equipment, it discloses using ring magnet, the lower terminal surface of reaction tube and ring magnet's up end butt, or in the hole of ring magnet was inserted to the bottom of reaction tube, avoided magnetism microballon to pile up in the reaction tube bottom entirely, but because ring magnet's magnetic field's distribution, magnetism microballon still mainly adsorbed on the lateral wall of reaction tube cone by the dispersibility, its adsorption range still is limited.

Disclosure of Invention

The utility model aims to provide a magnetic porous PCR plate, the shape of a magnet is matched with the shape of the cone part of a PCR tube, so that the gap between the magnet and the cone part of the PCR tube is reduced, the upper part of the magnet is positioned above the cone part of the PCR tube, the adsorption range is enlarged, and the magnetic attraction effect and the magnetic attraction efficiency are improved.

In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a magnetism porous PCR board, includes PCR board body, base, spacing post and magnet, be equipped with a plurality of constant head tanks that are used for embedding and support the bottom of PCR pipe on the base, be located every constant head tank's side on this base and be equipped with at least one magnet, magnet from bottom to top its cross section width reduces gradually, and its upper portion is higher than the up end of the cone of PCR pipe, a plurality of spacing post sets up in the week side of base upper end and is used for horizontal location PCR board body.

As a further optimization, the base comprises an upper plate and a lower plate which are in butt joint, the positioning groove and the limiting column are arranged on the upper plate, a limiting groove penetrating through the body of the base is formed in the side of the positioning groove on the upper plate, the lower part of the magnet is located in the limiting groove, the lower end of the magnet is in butt joint with the lower plate, and the upper part of the magnet protrudes out of the upper plate.

As further optimization, the side wall of the limit groove, which is close to one side of the limit groove, and/or the side wall of the limit groove, which is far away from one side of the limit groove, is provided with a locating surface matched with the side wall of the magnet, and the magnet can be prevented from being separated from the limit groove under the actions of the size of the lower part of the magnet and the locating surface.

As a further optimization, the positioning groove does not penetrate through the upper plate.

As further optimization, the lower end face of the upper plate is provided with a groove, the upper end of the lower plate is provided with a boss in a molding mode, the boss is embedded into the groove and is abutted to the lower end of the magnet, and the boss and the groove are matched to facilitate positioning and mounting of the lower plate and the upper plate.

As a further optimization, the upper plate and the lower plate are locked by bolts.

As further optimization, the magnet is a trapezoidal magnet, the trapezoidal structure is relatively easy to process, and the upper end and the lower end of the magnet are magnetized when magnetized, so that the N pole or the S pole at the two ends is realized, the stronger magnetic flux is arranged at the two ends, and the stronger magnetic attraction effect can be realized, so that the magnetic beads are adsorbed on the conical part of the PCR tube and the upper part of the conical part.

As further optimization, the number of the limiting columns is four, the horizontal section of the limiting columns is L-shaped, and the four limiting columns are respectively arranged at four corners of the base.

As further optimization, the upper end of the limiting column is provided with a guide inclined plane, so that the PCR plate body enters the space of the limiting column to be guided.

As a further optimization, the magnetic rack further comprises a cover plate, wherein a plurality of through holes for accommodating PCR tubes are formed in the cover plate, and the cover plate is covered on the base and is positioned above the magnets; the through holes are coaxially arranged with the positioning grooves. The cover plate can protect the magnets by placing a plurality of magnets inside.

Compared with the prior art, the utility model has the following beneficial effects:

1. the shape of the magnet is matched with the shape of the cone part of the PCR tube, so that the gap between the magnet and the cone part of the PCR tube is reduced, and the magnetic attraction effect can be improved;

2. the upper part of the magnet is positioned above the cone part of the PCR tube, so that the adsorption range is enlarged, and the magnetic attraction effect and the magnetic attraction efficiency are improved.

Drawings

Fig. 1 is a structural diagram of the present utility model.

FIG. 2 is a schematic diagram of the positioning slot, magnet and PCR tube positions according to the present utility model.

Fig. 3 is an exploded view of the present utility model.

Fig. 4 is an enlarged view at a in fig. 3.

Fig. 5 is a schematic view of the installation of a cover of another embodiment of the present utility model.

Fig. 6 is a block diagram of another embodiment of the present utility model.

Detailed Description

The following are specific embodiments of the present utility model and the technical solutions of the present utility model will be further described with reference to the accompanying drawings, but the present utility model is not limited to these embodiments.

As shown in fig. 1 to 2, a magnetic porous PCR plate comprises a PCR plate body 100, a base 1, a plurality of positioning slots 10 for embedding and supporting the bottom ends of PCR tubes 101 on the PCR plate body are provided on the base 1, at least one magnet 3 is provided on the base 1 beside each positioning slot 10, the magnet 3 can be a trapezoidal magnet, so that the width of the cross section of the magnet is gradually reduced from bottom to top, the upper part of the magnet is higher than the upper end surface of a cone 102 of the PCR tube 101, and a plurality of positioning posts 2 are provided on the periphery of the upper end of the base 1 for transversely positioning the PCR plate body 100.

In the utility model, the whole PCR plate body is stably placed on the base through the support of the limiting column and the base, and at the moment, the bottom end of the cone part of each PCR tube is positioned in the positioning groove, and the bottom end of the cone part of each PCR tube is positioned at the center of the bottom wall of the positioning groove and the positions of the bottom ends of the cone parts of each PCR tube in the positioning groove are consistent due to the limiting effect of the limiting column; because magnet from bottom to top becomes narrow gradually, so it can install between the cone of a pair of PCR pipe, compare in columnar, cubic magnet, have less clearance between the cone of this magnet and PCR pipe, can more advantageously exert its magnetic attraction effect to the magnetic microsphere, in addition, form bulge 3a on this magnet, it is located the top of the cone of PCR pipe, consequently can adsorb the magnetic microsphere in the vertical lateral wall department of PCR pipe, thereby realized adsorbing in the cone department of PCR pipe and the top department of cone, dispersed the absorption is favorable to improving magnetic attraction effect and efficiency to the magnetic microsphere. Further, the magnetic poles of the magnet of the present utility model are located at the upper and lower ends, so that the magnet has strong magnetic attraction at the two poles, which is also the reason for forming the protruding portion.

According to the utility model, the shape of the magnet is matched with the shape of the cone part of the PCR tube, so that the gap between the magnet and the cone part of the PCR tube is reduced, the magnetic attraction effect can be improved, and the magnetic attraction stability is ensured; the protruding part on the magnet can realize that the magnetic attraction is carried out above the conical part of the PCR tube, so that the scope of the adsorption effect is further enlarged, the magnetic attraction effect is avoided to be only generated at the conical part, and the magnetic attraction effect and the magnetic attraction efficiency are improved.

As shown in fig. 3 and 4 in combination with fig. 2, the base 1 includes an upper plate 11 and a lower plate 12 that are in contact with each other, the positioning groove 10 and the positioning post 2 are both disposed on the upper plate 11, a positioning groove 111 penetrating through the body of the positioning groove is disposed beside the positioning groove 10 on the upper plate 11, the lower part of the magnet 3 is disposed in the positioning groove 111, the lower end is in contact with the lower plate 12, and the upper part of the magnet 3 protrudes from the upper plate 11. For the convenience install a plurality of magnets in the side of constant head tank respectively, and because big-end-up's characteristics under the magnet, through setting up the spacing groove that runs through the upper plate, when installing magnet, invert the upper plate, then load into the spacing inslot with magnet inversion, lock down the board inversion with upper plate and magnet butt back, wholly overturn the back, just form the magnet protrusion in the up end of upper plate, accomplish the equipment of magnetic force frame, through installing the magnet in the spacing inslot can realize the stability of magnet position, avoid the mutual influence of magnetic pole between the magnet to lead to the magnet installation unstable.

The side wall of the side close to the positioning groove 10 and the side wall of the side far away from the positioning groove 10 in the limiting groove 111 are provided with positioning surfaces 112 matched with the side wall of the magnet 3, so that the magnet can be prevented from sliding downwards out of the limiting groove when being installed, and the magnet can be prevented from being separated from the limiting groove after being installed.

Preferably, the positioning groove 10 does not penetrate through the upper plate 11, and only the mounting opening of the limiting groove is formed on the lower end surface of the upper plate, so that the positioning is convenient when the magnet is mounted.

A groove (not shown) is formed in the lower end face of the upper plate 11, a boss 121 is formed in the upper end of the lower plate 12, the boss 121 is embedded into the groove and is abutted against the lower end of the magnet 3, and the boss is matched with the groove to achieve accurate and stable installation of the upper plate and the lower plate.

The number of the limiting columns 2 is four, the horizontal section of the limiting columns is L-shaped, the four limiting columns 2 are respectively arranged at four corners on the base 1, and the upper ends of the limiting columns 2 are provided with guide inclined planes 21, so that the PCR plate body can smoothly enter between the limiting columns and is abutted with the limiting columns to prevent the PCR plate body from transversely moving.

As shown in fig. 5 to 6, in another embodiment of the present utility model, only one magnet is disposed on one side of one positioning slot, which has better magnetic attraction to the magnetic microsphere, and can save cost (number of magnets) compared with the embodiment in which magnets are disposed on both sides of the positioning slot in the middle. In addition, the magnetic rack in the embodiment also comprises a cover plate 4, a plurality of through holes 40 for accommodating PCR tubes are arranged on the cover plate 4, and the cover plate 4 is covered on the base 1 and is positioned above the magnet 3; the through holes 40 and the positioning grooves 10 are coaxially arranged, and a plurality of PCR tubes can be respectively and accurately inserted into the through holes by means of the guiding action of the limiting columns during use. The cover plate can be installed (locked or in interference butt) between the positioning columns through the inserting angle 41 matched with the limiting columns on the cover plate body, and the lower end of the cover plate is in butt joint with the base, so that the magnet is arranged between the cover plates, on one hand, the coordination of the whole magnetic frame structure is improved, and on the other hand, the damage of the magnet caused by the exposure of the magnet can be avoided.

The specific embodiments described herein are offered by way of example only to illustrate the spirit of the utility model. Those skilled in the art may make various modifications or additions to the described embodiments or substitutions thereof without departing from the spirit of the utility model or exceeding the scope of the utility model as defined in the accompanying claims.

Claims

1. The utility model provides a magnetism porous PCR board, its characterized in that includes PCR board body, base, spacing post and magnet, be equipped with a plurality of constant head tanks that are used for embedding and support the bottom of PCR pipe on the base, be located every constant head tank's side on this base and be equipped with at least one magnet, magnet from bottom to top its cross section width reduces gradually, and its upper portion is higher than the up end of the cone of PCR pipe, a plurality of spacing post sets up in the week side of base upper end and is used for horizontal location PCR board body.

2. The magnetic multi-well PCR plate according to claim 1, wherein the base includes an upper plate and a lower plate that are in contact, the positioning groove and the limiting post are disposed on the upper plate, a limiting groove penetrating the body of the upper plate is disposed beside the positioning groove on the upper plate, the lower portion of the magnet is disposed in the limiting groove, the lower end of the magnet is in contact with the lower plate, and the upper portion of the magnet protrudes out of the upper plate.

3. The magnetic multi-well PCR plate according to claim 2, wherein the side wall of the limit groove near the side of the limit groove and/or the side wall of the limit groove far from the side of the limit groove is provided with a positioning surface matched with the side wall of the magnet.

4. A magnetic multi-well PCR plate according to claim 2 or 3, wherein the detent does not extend through the upper plate.

5. The magnetic multi-well PCR plate according to claim 2, wherein the lower end surface of the upper plate is provided with a groove, and the upper end of the lower plate is formed with a boss embedded in the groove and abutting against the lower end of the magnet.

6. The magnetic multi-well PCR plate of claim 2, wherein the upper plate and the lower plate are bolted.

7. The magnetic multi-well PCR plate according to claim 1, wherein the magnet is a trapezoidal magnet.

8. The magnetic multi-well PCR plate according to claim 1, wherein the number of the limit posts is four, the horizontal section of the limit posts is L-shaped, and the four limit posts are respectively arranged at four corners of the base.

9. The magnetic multi-well PCR plate of claim 8, wherein the upper end of the limit post is provided with a guide ramp.

10. The magnetic multi-well PCR plate of claim 1, further comprising a cover plate having a plurality of through holes for receiving PCR tubes, the cover plate covering the base and being positioned above the magnets; the through holes are coaxially arranged with the positioning grooves.