CN220402865U - Ex vivo pancreas oxygenation cryoperfusion system with phosphorylcholine coating - Google Patents
Ex vivo pancreas oxygenation cryoperfusion system with phosphorylcholine coating Download PDFInfo
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- CN220402865U CN220402865U CN202321774691.1U CN202321774691U CN220402865U CN 220402865 U CN220402865 U CN 220402865U CN 202321774691 U CN202321774691 U CN 202321774691U CN 220402865 U CN220402865 U CN 220402865U
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- Prior art keywords
- perfusate
- perfusion
- oxygenation
- pancreas
- phosphorylcholine
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- 210000000496 pancreas Anatomy 0.000 title claims abstract description 32
- YHHSONZFOIEMCP-UHFFFAOYSA-O phosphocholine Chemical compound C[N+](C)(C)CCOP(O)(O)=O YHHSONZFOIEMCP-UHFFFAOYSA-O 0.000 title claims abstract description 29
- 229950004354 phosphorylcholine Drugs 0.000 title claims abstract description 29
- 238000000576 coating method Methods 0.000 title claims abstract description 23
- 239000011248 coating agent Substances 0.000 title claims abstract description 22
- 238000006213 oxygenation reaction Methods 0.000 title claims abstract description 18
- 230000010412 perfusion Effects 0.000 claims abstract description 55
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000001301 oxygen Substances 0.000 claims abstract description 15
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims description 22
- 210000000056 organ Anatomy 0.000 claims description 16
- 230000002572 peristaltic effect Effects 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 12
- 238000001514 detection method Methods 0.000 claims description 11
- 210000001367 artery Anatomy 0.000 claims description 5
- 239000007789 gas Substances 0.000 claims description 5
- 210000003240 portal vein Anatomy 0.000 claims description 5
- 238000009825 accumulation Methods 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 abstract description 7
- 229920000642 polymer Polymers 0.000 abstract description 6
- 238000011084 recovery Methods 0.000 abstract description 6
- 230000006378 damage Effects 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000035899 viability Effects 0.000 abstract description 2
- 230000006870 function Effects 0.000 description 5
- 230000001603 reducing effect Effects 0.000 description 4
- ZSZRUEAFVQITHH-UHFFFAOYSA-N 2-(2-methylprop-2-enoyloxy)ethyl 2-(trimethylazaniumyl)ethyl phosphate Chemical compound CC(=C)C(=O)OCCOP([O-])(=O)OCC[N+](C)(C)C ZSZRUEAFVQITHH-UHFFFAOYSA-N 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 208000035143 Bacterial infection Diseases 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 210000001198 duodenum Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 206010019375 Helicobacter infections Diseases 0.000 description 1
- 241000521257 Hydrops Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 208000000718 duodenal ulcer Diseases 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 210000004153 islets of langerhan Anatomy 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000277 pancreatic duct Anatomy 0.000 description 1
- 230000004203 pancreatic function Effects 0.000 description 1
- 210000001819 pancreatic juice Anatomy 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000002627 tracheal intubation Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
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- External Artificial Organs (AREA)
Abstract
The utility model discloses an isolated pancreas oxygenation low-temperature perfusion system with a phosphorylcholine coating, which is provided with a power supply unit and a control panel connected with the power supply unit, wherein a perfusion container and a pipeline inner wall are arranged in a perfusion loop unit connected with the control panel. The utility model reduces the breeding of bacteria brought by pancreas donation body in the perfusion process through the special polymer coating, is beneficial to the recovery of patients and reduces the delay recovery of the transplanting function. The damage to pancreas is reduced by a low temperature oxygen carrying perfusion system, thereby improving the function of the isolated pancreas. Thereby improving pancreatic quality of the edge donor and improving islet viability.
Description
Technical Field
The utility model relates to the field of medical instruments, in particular to an in-vitro pancreas oxygenation low-temperature perfusion system with a phosphorylcholine coating.
Background
The low-temperature mechanical perfusion method continuously enters nutrient substances and oxygen into the pancreatic microcirculation through oxygen-carrying perfusion liquid, thereby providing tissue cell energy, maintaining the metabolic level of the tissue cell energy, removing the metabolites, reducing toxic substance accumulation, reducing injury and inflammation, reducing injury, protecting pancreatic functions, enabling long-distance transportation and long-time preservation, and medical clinical data show that the pancreatic islet functions of the pancreas are not destroyed even after 24 hours of low-temperature mechanical perfusion.
Perfusate flows in from the artery and out from the portal vein. Pancreatic juice is collected by intubation on the pancreatic duct, and the duodenum needs to be ligated to prevent perfusate from being polluted. The difficulty in pancreatic perfusion is that some donors themselves have different inflammations, such as duodenal ulcers, and most of them are caused by bacterial infection, the main bacterial infection is helicobacter pylori infection, and the bacteria are attached to the inner wall of the container in a low-temperature perfusion system and continuously proliferate, so that the bacteria content in the bacteria perfusate is obviously increased, and more bacteria are brought into the transplant receptor, and the probability of infection of the receptor is increased.
Disclosure of Invention
The utility model aims to provide an in-vitro pancreas oxygenation low-temperature perfusion system with a phosphorylcholine coating, which can reduce the infection probability of a transplant receptor and reduce the occurrence of complications.
In order to solve the technical problems, the embodiment of the utility model provides an in-vitro pancreas oxygenation low-temperature perfusion system with a phosphorylcholine coating, which is provided with a power supply unit and a control board connected with the power supply unit, wherein a perfusion circuit unit connected with the control board is connected with an organ groove for preserving pancreas, and a perfusion container and the inner wall of a pipeline are arranged in the perfusion circuit unit.
Further, the perfusate temperature of the perfusate circuit unit was 4 ℃.
Furthermore, the container is made of PC material, and the pipeline is made of PVC pipe.
Further, the perfusion circuit unit includes:
the liquid accumulator is provided with a first perfusate passage, a second perfusate passage and a third perfusate passage, and the first perfusate passage, the second perfusate passage and the third perfusate passage are respectively connected with the pancreatic portal vein, the pancreatic artery and the organ groove;
peristaltic pump connected to the organ tank through the fourth perfusate channel;
a filter connected to the peristaltic pump;
an oxygenator connected to the filter, the oxygenator connected to the effusion cell;
the oxygenator is connected with an oxygen generating system.
Further, the oxygen generating system comprises an oxygen generator, the oxygen generator is connected to the oxygen generator through a gas flow control unit, the gas flow control unit is connected to a flow control motor, and the flow control motor is connected to a control board.
Further, a signal acquisition section connected to the control board is also included, which includes:
a first flow rate sensor, a first bubble sensor, and a first pressure sensor disposed in the first perfusate channel;
a second flow sensor, a second bubble sensor, a second pressure sensor, disposed in the second perfusate channel;
the first temperature detection sensor and the second temperature detection sensor are arranged at two ends of the organ groove;
the third pressure sensor is arranged on the connection pipeline of the peristaltic pump and the filter;
and a third temperature detection sensor arranged in the liquid accumulation groove.
In the perfusate loop, a 2.5 mm conical vascular joint was used to connect to the portal vein and artery of the pancreas.
The utility model has the following beneficial effects:
1. the breeding of bacteria brought by pancreas donation bodies in the perfusion process is reduced through the polymer coating, the recovery of patients is facilitated, and the delayed recovery of the transplanting function is reduced.
2. The damage to pancreas is reduced by a low temperature oxygen carrying perfusion system, thereby improving the function of the isolated pancreas. Thereby improving pancreatic quality of the edge donor and improving islet viability.
Drawings
Fig. 1 is a schematic structural diagram of a phosphorylcholine material and a carrier for use in an ex vivo pancreas oxygenation cryoperfusion system with a phosphorylcholine coating according to a first embodiment of the utility model.
Fig. 2 is a schematic diagram of an ex vivo pancreas oxygenation cryoperfusion system for use with a phosphorylcholine coating according to a second embodiment of the utility model.
Detailed Description
The utility model is further described in connection with the following embodiments in order to make the technical means, the creation features, the achievement of the purpose and the effect of the utility model easy to understand.
A first embodiment of the present utility model provides an ex vivo pancreas oxygenation cryoperfusion system for use with a phosphorylcholine coating having a power supply unit and a control panel connected to the power supply unit, and a perfusion circuit unit connected to the control panel is connected to an organ tank for holding the pancreas, see fig. 1, wherein carrier 1 is a perfusion vessel and tubing of the perfusion circuit unit, and the perfusion vessel and tubing inner wall of the perfusion circuit unit is provided with phosphorylcholine material 2, such as 2-methacryloyloxyethyl phosphorylcholine produced by Sigma-Aldrich under merck flag, CAS No. 67881-98-5, molecular weight 295. An isolated pancreas oxygenation low temperature perfusion system based on a phosphorylcholine coating, which combines the condition that pancreas and duodenum possibly carry a large amount of germs, introduces an antibacterial coating of phosphorylcholine, thereby greatly reducing the infection risk.
In this embodiment, the carrier is made of PC or PVC, where the container is made of PC material, and the pipeline is made of PVC pipe.
The perfusion circuit unit of the present embodiment may refer to the prior art.
A second embodiment of the present utility model provides an ex-vivo pancreas oxygenation low temperature perfusion system with a phosphorylcholine coating (hereinafter referred to as "the system"), referring to fig. 2, having a power supply unit with a power supply management portion connected to a rechargeable battery and an ac-dc module, and a control board connected to a perfusion circuit unit, wherein a layer of phosphorylcholine material is disposed on a wall of the perfusion circuit unit and a wall of a pipe due to contact with the perfusate, and the phosphorylcholine material may be 2-methacryloyloxyethyl phosphorylcholine.
In this embodiment, the pipeline through which the perfusate flows is provided with the phosphorylcholine material, and the phosphorylcholine material is distributed throughout the inner wall of the non-pipeline portion, for example, the phosphorylcholine material is adopted at the places where the inner wall of the effusion cell, the organ tank, the peristaltic pump, the filter, the pinch valve, the oxygenator and the like contact the perfusate.
Referring to fig. 2, the perfusion circuit unit includes a effusion cell, the effusion cell has a first perfusion liquid passage (i.e. perfusion liquid passage 1 in the figure), a second perfusion liquid passage (perfusion liquid passage 2 in the figure), and a third perfusion liquid passage (perfusion liquid passage 3 in the figure), the first perfusion liquid passage and the second perfusion liquid passage are respectively connected to the portal vein of pancreas and the artery of pancreas, and the third perfusion liquid passage is connected to an organ groove, and is used as a bypass pipeline, and when perfusion liquid is not needed to be carried out on pancreas, the third perfusion liquid passage is used for ensuring the circulation of the perfusion circuit, and ensuring the stability and the safety of the system operation. And the peristaltic pump is connected with the organ groove through a fourth perfusate passage (perfusate passage 4 in the figure), the filter is connected with the peristaltic pump, the oxygenator is connected with the filter, and the oxygenator is connected with the effusion cell. The oxygenator is connected with the oxygen generating system. A 1/8 inch luer fitting with a barbed fixation may be adapted for use with the corresponding port of the perfusate passageway of this embodiment.
The oxygen generating system comprises an oxygen generator, wherein the oxygen generator is connected with the oxygen generator through a gas flow control unit, the gas flow control unit is connected with a flow control motor, and the flow control motor is connected with a control board. And the hydrops device is provided with a bubble catcher and a liquid level detector, and the liquid level detector is electrically connected with the control panel.
It should be noted that the system further comprises a signal acquisition part connected with the control board, wherein the signal acquisition part is mainly provided with a first flow rate sensor, a first bubble sensor and a first pressure sensor which are arranged in the first perfusate channel. The second perfusate channel is provided with a second flow rate sensor, a second bubble sensor and a second pressure sensor, the organ tank is provided with a first temperature detection sensor and a second temperature detection sensor for detecting temperatures at two ends of the organ tank, for example, the first temperature detection sensor detects temperatures at the inlet end of the organ tank, the second temperature detection sensor detects temperatures at the outlet end of the organ tank, the peristaltic pump and the filter connecting pipeline are provided with a third pressure sensor, and the liquid accumulation tank is provided with a third temperature detection sensor. The various sensors acquire corresponding signals and transmit the signals to the control board so that the system can operate according to a preset value, and the control board can keep a stable and safe working state of the system by controlling corresponding equipment to act after the preset value is exceeded.
The first perfusate passage, the second perfusate passage and the third perfusate passage are all provided with pinch valves connected with the control panel.
In conclusion, the product provided by the patent reduces the damage of pancreas through a low-temperature oxygen-carrying perfusion system, thereby improving the function of isolated pancreas. Thereby improving the pancreatic quality of the edge donor and improving the activity of the pancreas islet. Meanwhile, the breeding of bacteria brought by pancreas donation bodies in the perfusion process is reduced through the special polymer coating, so that the recovery of patients is facilitated, and the delayed recovery of the transplanting function is reduced.
The system is characterized in that oxygen with the purity of 93% is input into perfusion liquid stored at the temperature of 4 ℃, a peristaltic pump controlled by a single chip microcomputer generates 15-20 mmHg perfusion pressure in a perfusion pipeline, the perfusion flow rate is controlled at 25-32 ml/min through the peristaltic pump, the perfusion container and the pipeline are PVC pipe and PC material containers coated through a phosphorylcholine polymer coating process, phosphorylcholine polymer containing amino is dissolved in polar solvent to form polymer solution, a compound liquid stirrer is placed in the environment of 25 ℃ to mix and stir for 30 minutes at the speed of 400 revolutions per minute, the mixture is pumped into a 2-micrometer filter device through the peristaltic pump to ensure that pollutants are filtered, then the liquid is pumped into a local hundred-grade isolation operation room through the peristaltic pump, a worker accesses a coating solution interface through the pipeline to be coated through an isolation glove, and a valve is opened after two ends are connected, so that the phosphorylcholine solution flows through the inner wall of the pipe and is kept for at least 30 seconds. Airing for two hours in hundred-grade purifying environment after taking down.
Descriptions of the prior art are omitted throughout.
It will be understood by those of ordinary skill in the art that the foregoing embodiments are specific examples of carrying out the utility model and that various changes in form and details may be made therein without departing from the spirit and scope of the utility model.
Claims (6)
1. An isolated pancreas oxygenation low-temperature perfusion system with a phosphorylcholine coating, which is provided with a power supply unit and a control board connected with the power supply unit, wherein a perfusion loop unit connected with the control board is connected with an organ groove for preserving pancreas, and is characterized in that a perfusion container and the inner wall of a pipeline in the perfusion loop unit are provided with phosphorylcholine materials.
2. The ex vivo pancreatic oxygenation cryoperfusion system with phosphorylcholine coating according to claim 1, wherein the perfusate temperature of the perfusion circuit unit is 4 ℃.
3. The ex vivo pancreatic oxygenation cryoperfusion system with phosphorylcholine coating according to claim 1, wherein the vessel is PC material and the tubing is PVC tubing.
4. The ex vivo pancreatic oxygenation cryoperfusion system with phosphorylcholine coating according to claim 1, wherein the perfusion circuit unit comprises:
the liquid accumulator is provided with a first perfusate passage, a second perfusate passage and a third perfusate passage, and the first perfusate passage, the second perfusate passage and the third perfusate passage are respectively connected with the pancreatic portal vein, the pancreatic artery and the organ groove;
peristaltic pump connected to the organ tank through the fourth perfusate channel;
a filter connected to the peristaltic pump;
an oxygenator connected to the filter, the oxygenator connected to the effusion cell;
the oxygenator is connected with an oxygen generating system.
5. The ex vivo pancreas oxygenation cryoperfusion system with phosphorylcholine coating according to claim 4, wherein the oxygenation system comprises an oxygenator connected to the oxygenator through a gas flow control unit connected to a flow control motor connected to a control board.
6. The ex vivo pancreatic oxygenation cryoperfusion system with phosphorylcholine coating according to claim 4, further comprising a signal acquisition section connected to the control panel comprising:
a first flow rate sensor, a first bubble sensor, and a first pressure sensor disposed in the first perfusate channel;
a second flow sensor, a second bubble sensor, a second pressure sensor, disposed in the second perfusate channel;
the first temperature detection sensor and the second temperature detection sensor are arranged at two ends of the organ groove;
the third pressure sensor is arranged on the connection pipeline of the peristaltic pump and the filter;
and a third temperature detection sensor arranged in the liquid accumulation groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321774691.1U CN220402865U (en) | 2023-07-06 | 2023-07-06 | Ex vivo pancreas oxygenation cryoperfusion system with phosphorylcholine coating |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321774691.1U CN220402865U (en) | 2023-07-06 | 2023-07-06 | Ex vivo pancreas oxygenation cryoperfusion system with phosphorylcholine coating |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220402865U true CN220402865U (en) | 2024-01-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321774691.1U Active CN220402865U (en) | 2023-07-06 | 2023-07-06 | Ex vivo pancreas oxygenation cryoperfusion system with phosphorylcholine coating |
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| Country | Link |
|---|---|
| CN (1) | CN220402865U (en) |
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2023
- 2023-07-06 CN CN202321774691.1U patent/CN220402865U/en active Active
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