CN220250410U - Refrigerating device and cell culture equipment - Google Patents

Abstract

The application discloses refrigerating plant and cell culture equipment, refrigerating plant is used for refrigerating for the test tube, and refrigerating plant includes sleeve pipe subassembly, conducting part, refrigerating part, base and photoelectric sensor, and the sleeve pipe subassembly has the chamber that holds that is used for holding the test tube, holds the chamber and has the access & exit that supplies the test tube to come in and go out to hold the chamber; the conducting piece is sleeved on the peripheral side of the sleeve assembly and is connected with the sleeve assembly; the refrigerating piece is arranged on one side of the sleeve assembly, which is away from the access opening, and is connected with the sleeve assembly; the sleeve assembly and the conducting piece are detachably arranged on the base, the sleeve assembly is arranged behind the base, and the refrigerating piece is positioned between the base and the sleeve assembly; the photoelectric sensor sets up in the base, and photoelectric sensor is used for detecting whether the sleeve pipe subassembly is placed in the base. In this application embodiment, the refrigeration piece can be through sleeve pipe subassembly and conducting piece reduction hold the intracavity temperature fast when refrigerating to provide a low temperature preservation environment for the test tube.

Description

Refrigerating device and cell culture equipment

Technical Field

The application relates to the technical field of cell culture, in particular to a refrigerating device and cell culture equipment.

Background

With the development of life sciences, in order to better cope with various diseases, it has not been enough to satisfy animal models alone, and replication and reconstruction of human organs have become one of the directions of attempts by scientists, and cell and organoid culture techniques have become important. In the related art, it is necessary to perform low temperature quality preservation of a test tube or a reagent bottle containing biological tissue before or during cell culture, and if the temperature increases, the progress of cell culture may be affected.

Disclosure of Invention

The embodiment of the application provides a refrigerating plant and cell culture equipment, can realize holding the quick refrigeration in chamber to keep the reagent low temperature.

In a first aspect, an embodiment of the present application provides a refrigeration device at least for refrigerating a test tube, where the refrigeration device includes a sleeve assembly, a conductive member, a refrigeration member, a base, and a photoelectric sensor, the sleeve assembly having a housing cavity for housing the test tube, the housing cavity having an inlet and an outlet for the test tube to enter and exit the housing cavity; the conducting piece is sleeved on the peripheral side of the sleeve assembly and is connected with the sleeve assembly; the refrigerating piece is arranged on one side of the sleeve assembly, which is away from the access opening, and is connected with the sleeve assembly; the sleeve assembly and the conducting piece are detachably arranged on the base, the sleeve assembly is arranged behind the base, and the refrigerating piece is positioned between the base and the sleeve assembly; the photoelectric sensor is arranged on the base and is used for detecting whether the sleeve component is placed on the base or not; wherein, sleeve pipe subassembly and conducting piece all have the heat conductivity, and the refrigeration piece is used for refrigerating the test tube through sleeve pipe subassembly and conducting piece.

In some embodiments of the present application, the refrigeration device further comprises a heat insulating member located between the conductive member and the conductive tube, and the heat insulating member is disposed around a peripheral side of the conductive tube.

In some embodiments of the present application, the sleeve assembly includes a sleeve seat and a conductive pipe, and the sleeve assembly is disposed behind the base, and the refrigeration member is disposed between the base and the sleeve seat; the conducting pipe is arranged on one side of the sleeve seat far away from the refrigerating piece, the conducting pipe is provided with a containing cavity and an inlet and an outlet, the conducting piece is sleeved on the periphery of the conducting pipe, and the conducting piece is connected with the sleeve seat.

In some embodiments of the present application, adjacent first standing groove and second standing groove have been seted up on the base, and the sleeve pipe subassembly sets up behind the base, and the sleeve pipe seat is arranged in first standing groove, and the second standing groove is used for placing the reagent bottle.

In some embodiments of the present application, two photoelectric sensors are provided, a first through hole and a second through hole are formed in a side wall of the base, the first through hole is communicated with the first placing groove, at least part of the first photoelectric sensors in the two photoelectric sensors extend into the first through hole, and the first photoelectric sensors are used for detecting whether the cannula holder is placed in the first placing groove; the second through hole is communicated with the second placing groove, at least part of the second photoelectric sensors in the two photoelectric sensors extend into the second through hole, and the second photoelectric sensors are used for detecting whether the reagent bottle is placed in the second placing groove.

In some embodiments of the present application, the refrigeration device further includes a housing, the housing is sleeved on the base, the housing has a first through hole and a second through hole, the first through hole corresponds to a notch of the first placing groove, and the first through hole is used for passing the sleeve component and the conducting piece, so that the sleeve component and the conducting piece are placed in the first placing groove; the second through opening corresponds to the notch of the second placing groove, and is used for allowing the reagent bottle to pass through so as to place the reagent bottle in the second placing groove.

In some embodiments of the present application, the refrigeration device further comprises a water conduit in communication with the first and second placement grooves, the water conduit being for draining condensed water in the first and second placement grooves.

In some embodiments of the present application, the refrigeration device further comprises a temperature sensor disposed on the base, the temperature sensor being configured to detect a temperature of the sleeve assembly.

In some embodiments of the present application, the refrigeration member comprises a semiconductor refrigeration sheet, the semiconductor refrigeration sheet comprises a refrigeration surface and a heating surface which are arranged opposite to each other, and the refrigeration surface is connected with the sleeve assembly; the refrigerating device further comprises a heat radiating component which is connected with the heating surface and used for radiating heat of the heating surface of the refrigerating piece.

In a second aspect, a cell culture apparatus includes a body, a test tube, and a cooling device according to any of the above embodiments, where the cooling device is disposed in the body, and the test tube is disposed in a receiving chamber of the cooling device.

The beneficial effects of the embodiment of the application are that: in the embodiment of the application, the refrigerating part can quickly reduce the temperature in the accommodating cavity through the sleeve assembly and the conducting part when refrigerating, so that a low-temperature environment is provided for the test tube; moreover, because the test tube is arranged in the accommodating cavity, the sleeve component wraps the test tube, and the conduction piece wraps the sleeve component, the heat exchange between the accommodating cavity and the outside is reduced, so that the low-temperature environment in the accommodating cavity can be better maintained, and the low-temperature preservation of the test tube is facilitated. Meanwhile, in the embodiment of the application, the photoelectric sensor is arranged on the base, so that whether the sleeve assembly is placed on the base or not can be detected, automatic in-place detection of the refrigerating device is realized, the in-place detection efficiency of the sleeve assembly is improved, and the refrigerating efficiency of the test tube is improved.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of an exploded structure of a refrigeration device, test tube and reagent bottle according to an embodiment of the present application;

fig. 2 is a schematic structural diagram of a refrigerating device, a test tube and a reagent bottle according to an embodiment of the present application.

Reference numerals:

1. a refrigerating device; 10. a sleeve assembly; 11. an access opening; 12. a sleeve seat; 13. a conductive pipe; 20. a conductive member; 30. a refrigerating member; 40. a base; 41. a first placement groove; 42. a second placement groove; 51. a bracket; 52. an outer cover; 521. a first through port; 522. a second port; 53. a water conduit; 54. a temperature sensor; 55. a photoelectric sensor; 56. a heat dissipation assembly; 561. a metal heat sink; 562. a heat radiation fan; 201. a test tube; 202. reagent bottles.

Detailed Description

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the related art, the following description will be made in detail with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The culture of cells and organoids is one of the most commonly used means in biological and medical research, and the three-dimensional model such as organoids obtained by culture can be used in the fields of drug screening and accurate medical treatment, and in the process of automatic culture of cells/organoids (including reagent preparation stage), a test tube or a reagent bottle containing biological tissues is often required to be subjected to low-temperature (for example, 2-8 ℃) quality preservation, and if the temperature is too high, the process of cell culture is possibly affected. Meanwhile, in some cell culture experiments, a temperature-sensitive hydrogel is required, and in the early stage of cell culture, the temperature-sensitive hydrogel needs to be kept in a liquid state by low temperature, and if the temperature is too high, the form of the temperature-sensitive hydrogel cannot be maintained.

In view of the above, referring to fig. 1, the present application proposes a refrigeration device 1 for refrigerating at least a test tube 201, the refrigeration device 1 comprising a sleeve assembly 10, a conductive member 20, a refrigeration member 30, a base 40 and a photoelectric sensor 55.

The refrigerating apparatus 1 may refrigerate the test tube 201, and the refrigerating apparatus 1 may refrigerate the reagent bottle 202, the centrifuge tube, or the like. The cannula assembly 10 has a receiving chamber (not shown) for receiving the test tube 201, the receiving chamber having an access opening 11 for accessing the test tube 201. The sleeve assembly 10 has thermal conductivity, and when the temperature outside the sleeve assembly 10 is low, the sleeve assembly 10 can transfer cold to the inside, so that the temperature in the accommodating cavity is reduced, and the test tube 201 is located in the accommodating cavity, that is, the test tube 201 is wrapped by the sleeve assembly 10, and the test tube 201 is also in a low-temperature environment, so that the test tube 201 can be preserved at a low temperature. The sleeve assembly 10 may be made of metal, for example, because of its good heat conductivity.

The conductive member 20 is sleeved on the circumferential side of the sleeve assembly 10 and is connected with the sleeve assembly 10. The conductive member 20 surrounds the sleeve assembly 10, and the conductive member 20 has thermal conductivity, and on one hand, the conductive member 20 can uniformly transfer cold to the sleeve assembly 10; on the other hand, the conductive member 20 can reduce the direct contact of the sleeve assembly 10 with air, thereby reducing the heat transfer of the sleeve assembly 10 with the outside, and further can better maintain the low temperature environment in the accommodating chamber. Among them, the material of the conductive member 20 may be exemplified as a metal material because of its good heat conduction performance.

The refrigerating member 30 is disposed on a side of the sleeve assembly 10 facing away from the inlet/outlet 11 and is connected to the sleeve assembly 10, and the refrigerating member 30 is used for refrigerating the test tube 201 through the sleeve assembly 10 and the conducting member 20. The test tube 201 enters the accommodating cavity through the inlet and outlet 11, the temperature around the refrigerating element 30 is reduced when the refrigerating element 30 is refrigerated because the refrigerating element 30 is positioned at the bottom of the sleeve assembly 10, and the temperature in the accommodating cavity can be quickly reduced by the sleeve assembly 10 and the conducting element 20 because the sleeve assembly 10 and the conducting element 20 have heat conductivity, so that a low-temperature environment is provided for the test tube 201.

The sleeve assembly 10 and the conductive member are detachably disposed on the base 40, and the refrigerating member 30 is disposed between the base and the sleeve assembly 10 after the sleeve assembly 10 is disposed on the base 40. It will be appreciated that the base 40 provides a supporting and mounting space for the cooling member 30 and the sleeve assembly 10, the cooling member 30 is clamped between the base 40 and the sleeve assembly 10, and the cooling member 30 is cooled, so that after the temperature of the peripheral side is reduced, the cooling capacity is transferred to the sleeve assembly 10 and the conducting member 20, thereby reducing the temperature in the accommodating cavity and further providing a low-temperature storage environment for the test tube 201.

A photoelectric sensor 55 is provided on the base 40, and the photoelectric sensor 55 can detect whether the sleeve assembly 10 is placed on the base 40. For example, after the cuvette 201 is placed in the receiving chamber, the cannula assembly 10 is placed on the base 40, whereupon the photoelectric sensor 55 detects that the cannula assembly 10 is placed on the base 40.

It should be noted that, in the embodiment of the present application, the cooling element 30 can quickly reduce the temperature in the accommodating cavity through the sleeve assembly 10 and the conducting element 20 during cooling, so as to provide a low-temperature environment for the test tube 201; meanwhile, as the test tube 201 is positioned in the accommodating cavity, the sleeve assembly 10 wraps the test tube 201, and the conduction piece 20 wraps the sleeve assembly 10, heat exchange between the accommodating cavity and the outside is reduced, so that the low-temperature environment in the accommodating cavity can be better maintained, and the low-temperature preservation of the reagent in the test tube 201 is facilitated. Meanwhile, in the embodiment of the application, the photoelectric sensor 53 is arranged on the base 40, so that whether the sleeve assembly 10 is placed on the base 40 or not can be detected, automatic in-place detection of the refrigerating device 1 is realized, the in-place detection efficiency of the sleeve assembly 10 is improved, and the refrigerating efficiency of the test tube 201 is improved.

It should be further noted that the refrigeration apparatus 1 or the cell culture apparatus having the refrigeration apparatus 1 includes a controller electrically connected to the refrigeration member 30 and the photoelectric sensor 55, and when the photoelectric sensor 55 detects that the relevant device is placed on the base 40, the photoelectric sensor 55 sends an in-place detection signal to the controller, so that the controller controls the refrigeration member 30 to perform refrigeration.

Illustratively, the refrigeration device 1 may further include a bracket 51, where the bracket 51 is disposed on a side of the base 40 away from the casing assembly 10, and the bracket 51 is used to support the base 40, where the bracket 51 is disposed such that the base 40 and the casing assembly 10 are spaced apart from the body of the cell culture apparatus when the components are placed, so as to reduce the interference of the temperature of the body on the refrigeration device 1.

With continued reference to fig. 1, in some embodiments of the present application, the sleeve assembly 10 includes a sleeve mount 12 and a conductive pipe 13, and after the sleeve assembly 10 is disposed on the base 40, the refrigerating element 30 is disposed between the base 40 and the sleeve mount 12; the conducting tube 13 is arranged on one side of the sleeve seat 12 far away from the refrigerating piece 30, the conducting tube 13 is provided with a containing cavity, the conducting piece 20 is sleeved on the periphery side of the conducting tube 13, and the conducting piece 20 is connected with the sleeve seat 12.

Specifically, after the test tube 201 enters the accommodating cavity of the conducting tube 13 through the inlet and outlet 11, the conducting tube 13 can set the peripheral side of the test tube 201, and the conducting tube 13 is connected with the sleeve seat 12, that is, the sleeve seat 12 and the conducting tube 13 are surrounded to form the accommodating cavity with the inlet and outlet 11, the bottom of the accommodating cavity is the sleeve seat 12, the sleeve seat 12 bears the bottom of the test tube 201, the bottom of the test tube 201 in the accommodating cavity can be in contact with the sleeve seat 12, when the refrigerating piece 30 performs refrigeration, the sleeve seat 12 transfers cold for the bottom of the test tube 201, and meanwhile, the conducting tube 13 transfers cold for the side wall of the test tube 201, so that the temperature in the test tube 201 is rapidly reduced. It will be appreciated that the bottom of the test tube 201 may also be suspended in the receiving chamber.

It should be noted that, the conducting member 20 is sleeved on the peripheral side of the conducting tube 13, and the bottom of the conducting member 20 contacts with the upper end surface or the side surface of the sleeve seat 12, so that the cold on the sleeve seat 12 can be transferred to the conducting member 20, and the conducting member 20 can transfer the cold for the conducting tube 13 on one hand, and can reduce the heat exchange caused by the direct contact between the conducting tube 13 and the external environment on the other hand, so as to keep the conducting tube 13 warm. The overall shape of the sleeve holder 12 and the conductive tube 13 is not particularly limited, the overall shape of the conductive tube 13 is preferably long tube, the overall shape of the sleeve holder 12 is preferably square, the conductive tube 13 is disposed on the sleeve holder 12, the long tube-shaped conductive tube 13 can be more attached to the tube wall of the test tube 201, and the square sleeve holder 12 can be more stably placed to improve the stability of the overall structure.

In other embodiments, the conductive tube 13 is connected to the side of the cannula holder 12 remote from the refrigerating unit 30, the conductive tube 13 may have a first chamber therein, the cannula holder 12 may have a second chamber therein, the first chamber and the second chamber are in communication and together form a receiving chamber, the test tube 201 is located in the receiving chamber, an upper portion of the test tube 201 is located in the first chamber of the conductive tube 13, and a lower portion of the test tube 201 is located in the second chamber of the cannula holder 12.

Further, in some embodiments of the present application, the refrigeration device 1 further includes a heat insulation member (not shown) disposed around the periphery of the conductive pipe 13, so as to insulate the conductive pipe 13, and reduce heat exchange between the conductive pipe 13 and the external environment, so as to maintain the low-temperature environment in the conductive pipe 13 for a longer time. Meanwhile, the heat-insulating member is positioned between the conducting member 20 and the conducting pipe 13, and part of cold energy on the conducting member 20 can be transferred to the heat-insulating member, so that the heat-insulating effect of the heat-insulating member on the conducting pipe 13 is improved. Wherein, the heat preservation piece can be made of materials which can block heat flow transmission, such as heat preservation cotton, foam board or extruded sheet, etc.

With continued reference to fig. 1, in some embodiments of the present application, the base 40 is provided with a first placement groove 41 and a second placement groove 42 adjacent to each other, and after the sleeve assembly 10 is disposed on the base 40, the sleeve holder 12 is disposed in the first placement groove 41, and the second placement groove 42 is used for placing the reagent bottle 202.

It will be appreciated that the first placement groove 41 on the base 40 may provide a placement space for the casing holder 12, the side walls of the casing holder 12 may be located in the first placement groove 41, and the conductive member 20 is sleeved on the peripheral side of the casing holder 12, so that the lower end portion of the conductive member 20 may be located in the first placement groove 41, which may perform a limiting function on the conductive member 20 on one hand, and may also allow more cold energy to be transferred to the conductive member 20 on the other hand. Similarly, the second placement groove 42 on the base 40 can provide a placement space for the reagent bottle 202, and can limit the placement position of the reagent bottle 202.

In the process of performing cell culture, the refrigeration device 1 needs to perform low-temperature quality preservation on the biological tissue, the culture medium, and the reagent, so in the embodiment of the present application, not only the first placement groove 41 for performing low-temperature quality preservation on the test tube 201, but also the second placement groove 42 for performing low-temperature quality preservation on the reagent bottle 202 are provided.

Illustratively, the refrigerating member 30 may include a first refrigerating sheet disposed on the bottom wall of the first placing groove 41 and a second refrigerating sheet disposed on the bottom wall of the second placing groove 42, wherein the first refrigerating sheet is disposed between the bottom wall of the first placing groove 41 and the casing seat 12, and the sidewall of the first placing groove 41 is in contact with the casing seat 12, so that heat exchange between the casing seat 12 and the external environment can be reduced, and more cold energy in the first placing groove 41 is transferred to the casing seat 12. The second refrigerating piece is positioned between the bottom wall of the second placing groove 42 and the reagent bottle 202, and the cold generated by the second refrigerating piece is transferred to the bottom of the reagent bottle 202, so as to provide a low-temperature preservation environment for the reagent bottle 202; alternatively, a heat conductive sheet is provided in the second placement groove 42, and the cooling member 30 is located between the bottom wall of the second placement groove 42 and the heat conductive sheet, and the reagent bottle 202 is placed on the heat conductive sheet, and the heat conductive sheet uniformly transfers the cooling capacity to the reagent bottle 202. Of course, the refrigerating member 30 may be formed as a single piece, and a part of the refrigerating member 30 is in contact with the cannula holder 12 and another part of the refrigerating member 30 is in contact with the reagent bottle 202.

Further, in some embodiments of the present application, two photoelectric sensors 55 are provided, a first through hole and a second through hole are formed in a side wall of the base 40, the first through hole is communicated with the first placing groove 41, and a first photoelectric sensor in the two photoelectric sensors 55 at least partially extends into the first through hole, and is used for detecting whether the cannula holder 12 is placed in the first placing groove 41; the second through hole is communicated with the second placing groove 42, and a second photoelectric sensor of the two photoelectric sensors 55 at least partially extends into the second through hole and is used for detecting whether the reagent bottle 202 is placed in the second placing groove 42.

It can be understood that the first photoelectric sensor is disposed corresponding to the first placement groove 41, when the sleeve assembly 10 is placed in the first placement groove 41, the first photoelectric sensor can send the detected in-place signal of the sleeve assembly 10 to the controller, so that the controller controls the first refrigeration piece to refrigerate, and thus, the test tube 201 can be quickly refrigerated through the sleeve assembly 10 and the conductive piece 20; similarly, the second photoelectric sensor is disposed corresponding to the second placement groove 42, and when the reagent bottle 202 is placed in the second placement groove 42, the second photoelectric sensor can send the detected in-place signal of the reagent bottle 202 to the controller, so that the controller controls the second cooling plate to cool, and thus the reagent bottle 202 can be cooled rapidly through the second cooling plate.

Illustratively, taking a first photoelectric sensor as an example, a first reflective strip is disposed on a side wall of the ferrule holder 12, the first photoelectric sensor includes an emitting portion and a receiving portion, the emitting portion and the receiving portion are disposed toward the first through hole, when the ferrule assembly 10 is placed on the base 40, a position of the reflective strip on the ferrule holder 12 corresponds to a position of the first through hole, and light emitted by the emitting portion can be reflected by the reflective strip and then received via the receiving portion, so that the first photoelectric sensor detects that the heat conductive tube assembly 10 is placed in the first placement groove 41 of the base 40.

Further, please continue to refer to fig. 1, in some embodiments of the present application, the refrigeration apparatus 1 further includes an outer cover 52, the outer cover 52 is sleeved on the periphery of the base 40, the outer cover 52 mainly serves to insulate the refrigeration component 30 and the sleeve seat 12 on the base 40, and reduce the loss of cold air, so that the outer cover 52 may be made of insulating plastics, etc., and has not only an insulating function, but also a certain strength, so as to protect the internal devices. Wherein the housing 52 has a first through opening 521 and a second through opening 522, the first through opening 521 corresponding to a notch of the first placing groove 41, the first through opening 521 for passing the sleeve assembly 10 and the conductive member 20 therethrough to place the sleeve assembly 10 and the conductive member 20 in the first placing groove 41; the second through opening 522 corresponds to a notch of the second placement groove 42, and the second through opening 522 is used for the reagent bottle 202 to pass through, so that the reagent bottle 202 is placed in the second placement groove 42. During use of the refrigeration device 1, the sleeve assembly 10 and the conductive member 20 can be removed from the base 40 at any time via the first access opening 521, and the reagent bottle 202 can also be removed from the base 40 at any time via the second access opening 522. Of course, in other embodiments, the sleeve assembly 10 and the conductive member 20 may be fixedly coupled to the base 40.

Referring to fig. 1-2, in some embodiments of the present application, the refrigeration device 1 further includes a water conduit 53, the water conduit 53 is in communication with the first placement groove 41 and the second placement groove 42, and the water conduit 53 is used for draining the condensed water in the first placement groove 41 and the second placement groove 42.

It will be appreciated that when the cooling element 30 continues to exchange heat with the ambient air, the water vapor contained in the ambient air condenses on the surface of the cooling element 30 and forms dew, and when the dew increases to a certain extent, it slides into the first and second placement grooves 41 and 42, thereby forming condensed water, which is discharged through the water guide pipe 53, avoiding affecting the placement of the sleeve assembly 10 and the reagent bottle 202.

With continued reference to fig. 1-2, in some embodiments of the present application, the refrigeration apparatus 1 further includes a temperature sensor 54, where the temperature sensor 54 is disposed on the base 40, after the test tube 201 is placed in the accommodating cavity, the sleeve assembly 10 is placed on the base 40, and then the refrigeration member 30 performs refrigeration, and the cold energy is gradually transferred to the sleeve assembly 10, where the temperature sensor 54 can detect the real-time temperature of the sleeve assembly 10.

The type of the temperature sensor 54 is not particularly limited in the embodiment of the present application, for example, the temperature sensor 54 may be a contact type temperature sensor 54, the temperature sensor 54 includes a temperature measuring portion, when the sleeve assembly 10 is placed on the base 40, the sleeve seat 12 contacts with the temperature measuring portion, and thus the temperature sensor 54 detects the temperature of the sleeve assembly 10; of course, the temperature sensor 54 may be a non-contact temperature sensor 54.

Illustratively, the sidewall of the cannula holder 12 is provided with a third through hole, which is in communication with the receiving chamber, and the third through hole is used for the temperature measuring portion of the temperature sensor 54 to extend into, when the cannula assembly 10 is placed on the base 40, the temperature measuring portion extends into the receiving chamber via the third through hole, so as to detect the real-time temperature of the receiving chamber, so as to accurately control the heating temperature of the test tube 201.

It should be noted that, the refrigeration apparatus 1 or the controller in the cell culture apparatus having the refrigeration apparatus 1 is electrically connected to the temperature sensor 54, when the photoelectric sensor 55 detects that the relevant device is placed on the base 40, then the photoelectric sensor 55 sends an in-place detection signal to the controller; simultaneously or thereafter, the temperature sensor 54 detects that the relevant device is placed on the base 40, and then the temperature sensor 54 sends a temperature detection signal to the controller, so that the controller controls the cooling member 30 to perform cooling.

In some embodiments, a first placing groove 41 and a second placing groove 42 are formed in the base 40, the first cooling fin is located at the bottom wall of the first placing groove 41, the second cooling fin is located at the bottom wall of the second placing groove 42, two photoelectric sensors 55 and two temperature sensors 54 are provided, the first photoelectric sensors and the first temperature sensors are arranged corresponding to the first placing groove 41, when the sleeve assembly 10 is placed in the first placing groove 41, the first photoelectric sensors can send the detected in-place signal of the sleeve assembly 10 to the controller, and the first temperature sensors can send the detected temperature signal of the sleeve assembly 10 to the controller, so that the controller can control the first cooling fin to cool, and therefore the first cooling fin can rapidly cool the test tube 201 through the sleeve assembly 10 and the conducting piece 20 until the controller stops cooling after the first temperature sensor detects that the temperature of the sleeve assembly 10 reaches a preset value; similarly, the second photoelectric sensor and the second temperature sensor are arranged corresponding to the second placing groove 42, when the reagent bottle 202 is placed in the second placing groove 42, the second photoelectric sensor can send a detected in-place signal of the reagent bottle 202 to the controller, and the second temperature sensor can send a detected temperature signal of the reagent bottle 202 to the controller, so that the controller controls the second refrigerating sheet to refrigerate, and the reagent bottle 202 can be rapidly refrigerated through the second refrigerating sheet until the second temperature sensor detects that the temperature of the reagent bottle 202 reaches a preset value, and the controller stops refrigerating the first refrigerating sheet.

In some embodiments of the present application, the cooling element 30 comprises a semiconductor cooling plate, and the semiconductor cooling plate comprises a cooling surface and a heating surface which are arranged opposite to each other, and the cooling surface is connected with the sleeve assembly 10; the refrigeration device 1 further comprises a heat dissipation component 56, wherein the heat dissipation component 56 is connected with the heating surface and is used for dissipating heat of the heating surface of the refrigeration piece 30.

Specifically, the semiconductor refrigeration sheet cools through the refrigeration surface, and reduces the ambient temperature, so that the sleeve assembly 10 conducts cold into the accommodating cavity, and heat generated in the refrigeration process of the semiconductor refrigeration sheet is dissipated from the refrigeration surface and is dissipated through the heat dissipation assembly 56, so that the influence of the heat on the conduit assembly is reduced. The working principle of the semiconductor refrigeration sheet is disclosed in the related art, and the description of the working principle is omitted.

Illustratively, the heat dissipating component 56 includes a metal heat sink 561 and a heat dissipating fan 562, wherein the metal heat sink 561 absorbs heat generated by the heating surface and then transfers the heat to the external environment, thereby dissipating heat from the heating surface of the semiconductor cooling fin; then, the heat radiation fan 562 is aligned with the metal heat radiation fins 561, and heat transfer of the metal heat radiation fins 561 to the external environment is accelerated by increasing the gas flowing through the metal heat radiation fins 561.

In a second aspect, an embodiment of the present application further provides a cell culture apparatus (not shown in the drawings), including a body, a test tube 201, and a refrigeration device 1 as described in any of the foregoing embodiments, where the refrigeration device 1 is disposed in the body, and the test tube 201 is disposed in a receiving cavity of the refrigeration device 1.

It should be noted that, the test tube 201 is used for holding a culture medium containing biological tissue, the machine body is used for completing cell culture operation, in the process of performing cell culture, low temperature quality guarantee is needed for the culture medium containing biological tissue, or in the process of pre-preparation, the temperature sensitive hydrogel needs to be kept in a liquid state by low temperature, so that the test tube 201 containing biological tissue and the reagent bottle 202 containing reagent are placed on the refrigeration device 1, and the refrigeration device 1 rapidly refrigerates the test tube 201 and the reagent bottle 202 to ensure that the states of the biological tissue and the hydrogel are stable.

The same or similar reference numerals in the drawings of the present embodiment correspond to the same or similar components; in the description of the present application, it should be understood that, if there is an azimuth or positional relationship indicated by terms such as "upper", "lower", "left", "right", etc., based on the azimuth or positional relationship shown in the drawings, this is for convenience of description and simplification of the description, but does not indicate or imply that the components or elements referred to must have a specific azimuth, be configured and operated in a specific azimuth, and thus the terms describing the positional relationship in the drawings are merely for exemplary illustration, and should not be construed as limitations of the present application, and specific meanings of the terms described above may be understood by those of ordinary skill in the art according to specific circumstances.

The foregoing description of the preferred embodiment of the present utility model is not intended to limit the utility model to the particular form disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the utility model.

Claims (10)

1. A refrigeration device at least for refrigerating test tubes, said refrigeration device comprising:

a cannula assembly having a receiving chamber for receiving the test tube, the receiving chamber having an access port for the test tube to enter and exit the receiving chamber;

the conducting piece is sleeved on the peripheral side of the sleeve assembly and is connected with the sleeve assembly;

the refrigerating piece is arranged on one side of the sleeve assembly, which is away from the inlet and the outlet, and is connected with the sleeve assembly;

the sleeve assembly and the conducting piece are detachably arranged on the base, and the refrigerating piece is positioned between the base and the sleeve assembly after the sleeve assembly is arranged on the base;

the photoelectric sensor is arranged on the base and is used for detecting whether the sleeve assembly is placed on the base or not;

wherein, the sleeve pipe subassembly and the conducting piece all have the heat conductivity, the refrigeration piece is used for through the sleeve pipe subassembly and the conducting piece is right the test tube refrigeration.

2. The refrigeration unit of claim 1 wherein said sleeve assembly comprises:

the sleeve seat is arranged behind the base, and the refrigerating piece is positioned between the base and the sleeve seat;

the conducting pipe is arranged on one side, far away from the refrigerating piece, of the sleeve seat, the conducting pipe is provided with the accommodating cavity, the conducting piece is sleeved on the periphery of the conducting pipe, and the conducting piece is connected with the sleeve seat.

3. The refrigeration unit of claim 2, further comprising:

the heat preservation piece is located between the conducting piece and the conducting pipe, and the heat preservation piece surrounds the periphery of the conducting pipe.

4. The refrigeration unit of claim 2, wherein the base is provided with a first placement groove and a second placement groove adjacent to each other, the sleeve assembly is disposed on the base, the sleeve seat is disposed in the first placement groove, and the second placement groove is used for placing a reagent bottle.

5. The refrigeration unit of claim 4 wherein said photosensor is provided in two, and the side wall of said base is provided with:

the first through holes are communicated with the first placing grooves, at least part of first photoelectric sensors in the two photoelectric sensors extend into the first through holes, and the first photoelectric sensors are used for detecting whether the sleeve seat is placed in the first placing grooves or not;

the second through holes are communicated with the second placing grooves, at least part of the second photoelectric sensors in the two photoelectric sensors extend into the second through holes, and the second photoelectric sensors are used for detecting whether the reagent bottles are placed in the second placing grooves or not.

6. The refrigeration unit of claim 4, further comprising:

the outer cover is sleeved on the base and is provided with a first through hole and a second through hole, the first through hole corresponds to the notch of the first placing groove, and the first through hole is used for allowing the sleeve assembly and the conducting piece to pass through so as to place the sleeve assembly and the conducting piece in the first placing groove; the second through port corresponds to a notch of the second placing groove, and is used for allowing the reagent bottle to pass through so as to place the reagent bottle in the second placing groove.

7. The refrigeration unit of claim 4, further comprising:

and the water guide pipe is communicated with the first placing groove and the second placing groove and is used for discharging condensed water in the first placing groove and the second placing groove.

8. The refrigeration unit of claim 1, further comprising:

the temperature sensor is arranged on the base and is used for detecting the temperature of the sleeve assembly.

9. The refrigeration unit as recited in any one of claims 1 to 8 wherein said refrigeration member includes a semiconductor refrigeration sheet including a refrigeration face and a heating face disposed opposite each other, said refrigeration face being connected to said sleeve assembly; the refrigeration device further comprises:

and the heat dissipation component is connected with the heating surface and is used for dissipating heat of the heating surface of the refrigerating piece.

10. A cell culture apparatus comprising a body, a test tube and a refrigeration device according to any one of claims 1 to 9, the refrigeration device being disposed within the body, the test tube being disposed in the receiving cavity of the refrigeration device.