CN220238337U - Full-automatic protein purification device is used in blocker processing - Google Patents
Full-automatic protein purification device is used in blocker processing Download PDFInfo
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- CN220238337U CN220238337U CN202321735274.6U CN202321735274U CN220238337U CN 220238337 U CN220238337 U CN 220238337U CN 202321735274 U CN202321735274 U CN 202321735274U CN 220238337 U CN220238337 U CN 220238337U
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- mixing box
- liquid
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- cleaning
- purification device
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- 238000001742 protein purification Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 89
- 238000002156 mixing Methods 0.000 claims abstract description 49
- 238000004140 cleaning Methods 0.000 claims abstract description 47
- 238000003756 stirring Methods 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 238000000746 purification Methods 0.000 claims abstract description 18
- 238000009434 installation Methods 0.000 claims description 10
- 238000011010 flushing procedure Methods 0.000 claims description 8
- 239000002981 blocking agent Substances 0.000 claims description 7
- 239000002699 waste material Substances 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 230000003014 reinforcing effect Effects 0.000 claims description 4
- 238000003466 welding Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 8
- 230000000149 penetrating effect Effects 0.000 abstract description 4
- 238000010438 heat treatment Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 230000005484 gravity Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 239000000556 agonist Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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Abstract
The utility model discloses a full-automatic protein purification device for blocker processing, which comprises a mounting transverse plate, wherein the left side of the top of the mounting transverse plate is connected with a mixing box through a bolt, the left side of the mixing box is connected with a stirring mechanism through a bolt, the top of the mixing box is fixedly connected with a cleaning mechanism in a penetrating manner, the bottoms of the left side and the right side of an inner cavity of the mixing box are fixedly connected with heaters in a penetrating manner through bolts, the rear side of the inner cavity of the mixing box is connected with a temperature sensor through bolts, the top and the bottom of the left side of the inner cavity of the mixing box are connected with liquid level sensors through bolts, the front side of the bottom of the mixing box is communicated with a liquid pump, and a water outlet pipe of the liquid pump is communicated with purification equipment. The utility model has the advantage of accurate temperature control through the cooperation of the mixing box, the controller, the temperature sensor and the heater, can accurately control the heating temperature of the mixed liquid, and has better purification effect.
Description
Technical Field
The utility model relates to the technical field of blocker processing, in particular to a full-automatic protein purification device for blocker processing.
Background
Blocking agents are pharmacological terms which refer to a class of ligand substances which bind to receptors and prevent the effect of an agonist, and which require the use of protein purification devices to separate and purify the desired protein of interest from the mixture during processing.
The utility model patent of the patent application publication number CN213680478U discloses a protein purification device, which comprises a box body, wherein a plurality of gravity chromatographic columns are arranged, so that the gravity chromatographic columns can work simultaneously, the purification efficiency can be effectively improved, and a top cover is arranged at a feed inlet of the box body, so that other impurities in the air can be effectively prevented from polluting protein raw materials; a driving motor and a stirring shaft are arranged in the liquid storage cavity, and the driving motor drives the stirring shaft to rotate, so that the organic solvent in the liquid storage cavity is fully mixed with the protein stock solution, and the purification effect is improved; the protein purification device designed by the utility model can improve the purification efficiency, and is suitable for rapid and standardized experimental work development in ELISA laboratories.
However, the device still has some defects when in actual use, and obviously, the temperature of the mixture cannot be accurately controlled, the mixture cannot be accurately heated in the process of mixing the protein stock solution and the organic solution, so that the purification effect is not ideal, the device does not have an automatic cleaning function, personnel are required to manually clean parts in the device, the cleaning efficiency is low, and the working intensity of the personnel is increased.
Therefore, it is necessary to invent a fully automatic protein purification device for blocker processing to solve the above problems.
Disclosure of Invention
The utility model aims to provide a full-automatic protein purification device for blocker processing, which has the function of accurate temperature control, has better purification effect, has the function of automatic cleaning and reduces the working intensity of personnel, and solves the problems that the device does not have the function of accurate temperature control, has poor purification effect, does not have the function of automatic cleaning and increases the working intensity of personnel.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a full-automatic protein purification device is used in blocker processing, includes the installation diaphragm, the left side at installation diaphragm top is through bolted connection has the mixing box, there is rabbling mechanism in the left side of mixing box through bolted connection, the top of mixing box runs through fixedly connected with wiper mechanism, the bottom of mixing box inner chamber left and right sides all is through the bolt fixedly connected with heater, there is temperature sensor at the rear side of mixing box inner chamber through bolted connection, the top of mixing box inner chamber left side and bottom all are through bolted connection have level sensor, the front side intercommunication of mixing box bottom has the drawing liquid pump, the outlet pipe intercommunication of drawing liquid pump has purifying equipment, the bottom intercommunication of purifying equipment has the flowing back valve, the bottom intercommunication of flowing back valve has the liquid reserve tank.
Preferably, the top of the purifying device is communicated with a liquid inlet valve, the top of the liquid inlet valve is communicated with a water outlet pipe of the liquid drawing pump, and the left side and the right side of the top of the mixing box are both communicated with liquid adding pipes.
Preferably, the front side of the purifying device is connected with a controller through a bolt, the output end of the controller is electrically connected with the input ends of the stirring mechanism, the cleaning mechanism, the liquid pump and the heater respectively, and the output ends of the liquid level sensor and the temperature sensor are electrically connected with the input ends of the controller.
Preferably, the stirring mechanism comprises a stirring motor, the output end of the stirring motor is fixedly connected with a rotating rod, the right side of the rotating rod penetrates through the right side of the inner cavity of the mixing box, a stirring rod is sleeved on the surface of the rotating rod, and stirring teeth are welded on the surface of the stirring rod.
Preferably, the cleaning mechanism comprises a cleaning liquid tank, a cleaning water pump is communicated with the left side of the cleaning liquid tank, a connecting water pipe is communicated with the water outlet end of the cleaning water pump, an annular flushing pipe is communicated with one side of the connecting water pipe away from the cleaning water pump, the bottom of the annular flushing pipe penetrates through the inner cavity of the mixing tank, and a liquid filling hopper is communicated with the front side of the top of the cleaning liquid tank.
Preferably, the rear side intercommunication of mixing box bottom has the fluid-discharge tube, the surface cover of fluid-discharge tube is established and is linked together there is the waste liquid valve, the four corners of installation diaphragm bottom has all been welded the support column, and the bottom welding between two left and right sides support column has the reinforcing diaphragm.
Compared with the prior art, the utility model has the following beneficial effects:
the utility model has the advantage of accurate temperature control through the cooperation of the mixing box, the controller, the temperature sensor and the heater, can accurately control the heating temperature of the mixed liquid, and has better purification effect.
The utility model has the advantages of automatic cleaning by the cooperation of the cleaning water pump, the annular flushing pipe, the connecting water pipe and the cleaning liquid tank, does not need personnel to manually clean the inside of the mixing tank, reduces the working intensity of personnel and improves the cleaning efficiency.
Drawings
FIG. 1 is an isometric view of a construction of the present utility model;
FIG. 2 is an interior isometric view of a mixing tank of the present utility model;
FIG. 3 is a rear isometric view of a partial structure of the present utility model;
FIG. 4 is a right side isometric view of the purification apparatus and the liquid storage tank of the present utility model.
In the figure: 1. mounting a transverse plate; 2. a support column; 3. a mixing box; 4. a stirring mechanism; 5. cleaning a water pump; 6. a liquid adding tube; 7. a cleaning mechanism; 8. a liquid inlet valve; 9. a purification device; 10. a controller; 11. a liquid discharge valve; 12. a liquid storage tank; 13. a temperature sensor; 14. a heater; 15. a liquid injection hopper; 16. a liquid pump; 17. a stirring rod; 18. a rotating rod; 19. a stirring motor; 20. stirring teeth; 21. a liquid level sensor; 22. a waste liquid valve; 23. a liquid discharge pipe; 24. an annular flushing pipe; 25. connecting a water pipe; 26. a cleaning liquid tank.
Detailed Description
Referring to fig. 1-4, a full-automatic protein purification device for blocker processing comprises a mounting transverse plate 1, wherein the left side of the top of the mounting transverse plate 1 is connected with a mixing box 3 through a bolt, the left side of the mixing box 3 is connected with a stirring mechanism 4 through a bolt, the top of the mixing box 3 is fixedly connected with a cleaning mechanism 7 in a penetrating manner, the bottoms of the left side and the right side of an inner cavity of the mixing box 3 are fixedly connected with a heater 14 in a penetrating manner through bolts, the rear side of the inner cavity of the mixing box 3 is connected with a temperature sensor 13 through bolts, the top and the bottom of the left side of the inner cavity of the mixing box 3 are connected with a liquid extracting pump 21 through bolts, the front side of the bottom of the mixing box 3 is communicated with a water outlet pipe of the liquid extracting pump 16 is communicated with a purification device 9, the bottom of the purification device 9 is communicated with a liquid draining valve 11, and the bottom of the liquid draining valve 11 is communicated with a liquid storing box 12;
the top of the purification equipment 9 is communicated with a liquid inlet valve 8, the top of the liquid inlet valve 8 is communicated with a water outlet pipe of a liquid drawing pump 16, the left side and the right side of the top of the mixing box 3 are both communicated with a liquid adding pipe 6, and the purification equipment 9 can not be closed by arranging the liquid inlet valve 8;
the front side of the purifying device 9 is connected with a controller 10 through bolts, the output end of the controller 10 is respectively and electrically connected with the input ends of the stirring mechanism 4, the cleaning mechanism 7, the liquid pump 16 and the heater 14, and the output ends of the liquid level sensor 21 and the temperature sensor 13 are respectively and electrically connected with the input end of the controller 10;
the stirring mechanism 4 comprises a stirring motor 19, the output end of the stirring motor 19 is fixedly connected with a rotating rod 18, the right side of the rotating rod 18 penetrates through the right side of the inner cavity of the mixing box 3, a stirring rod 17 is fixedly sleeved on the surface of the rotating rod 18, stirring teeth 20 are welded on the surface of the stirring rod 17, and the stirring motor has a stirring function through the arrangement of the stirring teeth 20, so that the raw materials can be mixed more uniformly;
the cleaning mechanism 7 comprises a cleaning solution tank 26, the left side of the cleaning solution tank 26 is communicated with a cleaning water pump 5, the water outlet end of the cleaning water pump 5 is communicated with a connecting water pipe 25, one side of the connecting water pipe 25 away from the cleaning water pump 5 is communicated with an annular flushing pipe 24, the bottom of the annular flushing pipe 24 penetrates through the inner cavity of the mixing tank 3, the front side of the top of the cleaning solution tank 26 is communicated with a liquid injection hopper 15, and the cleaning solution tank has the function of conveniently adding liquid by arranging the liquid injection hopper 15, so that the problem that cleaning solution cannot be filled into the cleaning solution tank 26 is solved;
the rear side intercommunication of mixing box 3 bottom has fluid-discharge tube 23, the surface cover of fluid-discharge tube 23 establishes the intercommunication and has waste liquid valve 22, the four corners of installation diaphragm 1 bottom has all welded support column 2, and the bottom welding between two left and right sides support column 2 has the reinforcing diaphragm, through setting up the reinforcing diaphragm, possesses the fixed function of installation, has solved unable problem of installing fixedly to wash water pump 5 and washing liquid tank 26.
When the device is used, raw materials enter the mixing box 3 through the liquid adding pipes 6 on two sides, when the liquid level of the raw materials in the mixing box 3 is higher than the liquid level sensor 21 at the top, signals are transmitted to the controller 10, personnel stop feeding the liquid adding pipes 6 through observing the controller 10, then the stirring motor 19 and the heater 14 are controlled to work, the stirring motor 19 drives the stirring rod 17 on the surface of the rotating rod 18 to rotate so as to stir the mixed liquid, the mixed liquid is uniformly mixed, the heater 14 works to heat the mixed liquid, the temperature sensor 13 detects the heated temperature and transmits the signals to the controller 10, numerical values are displayed on the controller 10, after the temperature sensor 13 detects the temperature to be lower than the preset value, the controller 10 controls the heater 14 to work again to heat the mixed liquid, then the liquid pump 16 is controlled to work to convey the mixed liquid in the purifying equipment 9, after the purification is finished, the liquid flows into the liquid storage box 12 to be stored, when the liquid storage box is used, the inside the mixing box 3 needs to be heated, the liquid storage box is required to be cleaned, the liquid storage box is opened, the washing liquid is more quickly flows into the liquid storage box through the annular spray pipe 25, the inner part of the cleaning liquid storage box is cleaned, and the inner wall of the cleaning liquid is cleaned by the annular pipe is cleaned through the annular spray pipe 25, and the inner part is more conveniently cleaned by the cleaning liquid pipe 24, and the inner part is cleaned by the cleaning liquid is more conveniently cleaned, and the inner part is cleaned, and the inner part of the cleaning liquid is more completely cleaned, and the waste is more has the cleaning liquid.
To sum up: this full-automatic protein purification device is used in blocker processing uses through the cooperation of mixing box 3, controller 10, temperature sensor 13, heater 14, wash water pump 5, annular wash pipe 24, connecting water pipe 25 and washing liquid tank 26, has solved not possess the function of accurate accuse temperature, and purifying effect is not good, does not possess the self-cleaning function, has increased personnel working strength's problem.
Claims (6)
1. The utility model provides a blocking agent processing is with full-automatic protein purification device, includes installation diaphragm (1), its characterized in that: the utility model discloses a liquid pump, including installation diaphragm (1), including installation diaphragm, liquid pump, purifying equipment (9), drain valve (11), drain valve (12) are had in the bottom intercommunication of (3) inner chamber, the left side at installation diaphragm (1) top has mixing box (3) through bolted connection, the left side of mixing box (3) has mixing box (3) through bolted connection through the bolted connection, the left side of mixing box (3) has stirring mechanism (4) through bolted connection, the top of mixing box (3) has in the front side intercommunication of bottom has drawing liquid pump (16), the outlet pipe intercommunication of drawing liquid pump (16) has purifying equipment (9), the bottom intercommunication of mixing box (3) inner chamber left and right sides has heater (14) through the bolt.
2. The fully automatic protein purification device for processing a blocking agent according to claim 1, wherein: the top of purification equipment (9) is communicated with a liquid inlet valve (8), the top of the liquid inlet valve (8) is communicated with a water outlet pipe of a liquid pump (16), and the left side and the right side of the top of the mixing box (3) are both communicated with a liquid adding pipe (6).
3. The fully automatic protein purification device for processing a blocking agent according to claim 1, wherein: the front side of purification equipment (9) is connected with a controller (10) through a bolt, the output end of the controller (10) is electrically connected with the input ends of a stirring mechanism (4), a cleaning mechanism (7), a liquid pump (16) and a heater (14) respectively, and the output ends of a liquid level sensor (21) and a temperature sensor (13) are electrically connected with the input ends of the controller (10).
4. The fully automatic protein purification device for processing a blocking agent according to claim 1, wherein: the stirring mechanism (4) comprises a stirring motor (19), the output end of the stirring motor (19) is fixedly connected with a rotating rod (18), the right side of the rotating rod (18) penetrates through the right side of the inner cavity of the mixing box (3), a stirring rod (17) is sleeved on the surface of the rotating rod (18), and stirring teeth (20) are welded on the surface of the stirring rod (17).
5. The fully automatic protein purification device for processing a blocking agent according to claim 1, wherein: the cleaning mechanism (7) comprises a cleaning liquid tank (26), a cleaning water pump (5) is communicated with the left side of the cleaning liquid tank (26), a connecting water pipe (25) is communicated with the water outlet end of the cleaning water pump (5), an annular flushing pipe (24) is communicated with one side, far away from the cleaning water pump (5), of the connecting water pipe (25), the bottom of the annular flushing pipe (24) penetrates through the inner cavity of the mixing tank (3), and a liquid injection hopper (15) is communicated with the front side of the top of the cleaning liquid tank (26).
6. The fully automatic protein purification device for processing a blocking agent according to claim 1, wherein: the rear side of mixing box (3) bottom intercommunication has fluid-discharge tube (23), the surface cover of fluid-discharge tube (23) is established the intercommunication and is had waste liquid valve (22), the four corners of installation diaphragm (1) bottom has all been welded support column (2), and the bottom welding between two support column (2) has the reinforcing diaphragm about.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321735274.6U CN220238337U (en) | 2023-07-04 | 2023-07-04 | Full-automatic protein purification device is used in blocker processing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321735274.6U CN220238337U (en) | 2023-07-04 | 2023-07-04 | Full-automatic protein purification device is used in blocker processing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220238337U true CN220238337U (en) | 2023-12-26 |
Family
ID=89234962
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321735274.6U Active CN220238337U (en) | 2023-07-04 | 2023-07-04 | Full-automatic protein purification device is used in blocker processing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220238337U (en) |
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2023
- 2023-07-04 CN CN202321735274.6U patent/CN220238337U/en active Active
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