CN220207983U - Biological microscope for synchronously observing positive and bottom views of living single cells - Google Patents

Abstract

The utility model discloses a biological microscope for synchronously observing the front and bottom views of living single cells, which comprises a bearing seat, a triaxial moving platform, a zoom lens observation device, an electronic light source assembly, an objective table assembly, an microscopic group observation device and a platform protection cover. The problem that the existing biological microscope can only obtain one view of the cells and can not accurately obtain the characteristic information of the cells in the thickness direction is solved.

Description

Biological microscope for synchronously observing positive and bottom views of living single cells

Technical Field

The utility model belongs to the technical field of inspection instruments, and particularly relates to a biological microscope for synchronously observing the positive and bottom views of living single cells.

Background

Microscopes are widely used in biology and medicine, and are important tools for understanding the microscopic world. With the multi-scale development of modern biomedicine, the biological sample to be observed already reaches the micro-nano scale, and a microscope with higher observation precision needs to be developed. More than 80% of external information is acquired through eyes, so that the acquisition of three-dimensional information has important significance for human perception of the external world. The time is advancing continuously, the requirement of human on information is increasing continuously, the objective world is three-dimensional, the requirement of human is not met gradually, and therefore high-precision three-dimensional observation and clear display of objects are being pursued. At present, most domestic microscopes perform plane observation on a sample, the observation on three-dimensional information of the sample is less, and the development of a three-dimensional stereo microscope is not mature enough. In order to save cost and improve observation accuracy, a biological microscope capable of synchronously observing the front and bottom views of a living single cell is designed, and the biological microscope can be used for acquiring three-dimensional shape and size information of the single cell and improving the efficiency of observing the single cell morphology.

Disclosure of Invention

Aiming at the problems that the prior microscope can not obtain cell thickness direction information and low observation efficiency from a single view angle, the utility model provides a biological microscope for synchronously observing the positive and bottom views of living single cells, which is provided with two independent observation devices, can simultaneously obtain the positive and bottom views of the living single cells, can obtain the length, width and thickness three-dimensional geometric information of the living single cells through subsequent processing, effectively solves the problems, and is convenient for operators to use.

The utility model provides the following technical scheme: the utility model provides a biological microscope of synchronous observation living body unicellular positive, bottom view, includes the bearing seat, bearing seat top left side is connected with XYZ triaxial moving platform one, XYZ triaxial moving platform one top is connected the zoom lens observation device, bearing seat top front side is connected with XYZ triaxial moving platform two, XYZ triaxial moving platform two tops are connected the electron light source subassembly, bearing seat top rear side is connected with XYZ triaxial moving platform three, XYZ triaxial moving platform three tops are connected the mirror and are examined group observation device, bearing seat top middle part is connected with objective table subassembly, four apex angle departments above the bearing seat are connected with the lower cover body of platform safety cover through the bolted connection, the lower cover body top of platform safety cover has the upper cover body of platform safety cover through the recess joint.

Further, the bearing seat comprises an aluminum alloy 6061 bottom plate, and the aluminum alloy 6061 bottom plate is connected with four conical foot pads through bolts.

Further, the first XYZ three-axis moving platform is connected with an aluminum alloy 6061 base plate through a bolt, the first XYZ three-axis moving platform is provided with an X-axis measuring head, the X-axis measuring head is connected with a rotary rod 1 through a jackscrew, the rotary rod 1 is connected with a first bevel gear through the jackscrew, two bevel gears are meshed, the other bevel gear is connected with a rotary rod 4 through the jackscrew, the rotary rod 4 is connected with a third scale trimming hand wheel through the jackscrew, the first XYZ three-axis moving platform is provided with a Y-axis measuring head, the Y-axis measuring head is connected with a rotary rod 3 through the jackscrew, the rotary rod 3 is connected with a second scale trimming hand wheel, the first XYZ three-axis moving platform is provided with a Z-axis measuring head, the Z-axis measuring head is connected with a rotary rod 2 through the jackscrew, and the rotary rod 2 is connected with the first scale trimming hand wheel through the jackscrew. The X-axis measuring head is arranged on the second XYZ-axis moving platform, the X-axis measuring head is connected with the rotary rod 5 through a jackscrew, the rotary rod 5 is connected with the second bevel gear through a jackscrew, the two bevel gears are meshed, the other bevel gear is connected with the rotary rod 8 through a jackscrew, the rotary rod is connected with the six scale trimming handwheels through a jackscrew, the Y-axis measuring head is arranged on the second XYZ-axis moving platform, the Y-axis measuring head is connected with the rotary rod 7 through a jackscrew, the rotary rod 7 is connected with the five scale trimming handwheels through a jackscrew, the Z-axis measuring head is arranged on the second XYZ-axis moving platform, the Z-axis measuring head is connected with the rotary rod 6 through a jackscrew, and the rotary rod 6 is connected with the four scale trimming handwheels through a jackscrew. The X-axis measuring head is arranged on the X-axis moving platform II, the X-axis measuring head is connected with the rotary rod 9 through a jackscrew, the rotary rod 9 is connected with the bevel gears II through jackscrews, the two bevel gears are meshed, the other bevel gear is connected with the rotary rod 12 through jackscrews, the rotary rod 12 is connected with the scale fine adjustment hand wheel II through jackscrews, the Y-axis measuring head is arranged on the X-axis moving platform II, the Y-axis measuring head is connected with the rotary rod 11 through jackscrews, the rotary rod 11 is connected with the scale fine adjustment hand wheel II through jackscrews, the Z-axis measuring head is arranged on the X-axis moving platform II, the Z-axis measuring head is connected with the rotary rod 10 through jackscrews, and the rotary rod 10 is connected with the scale fine adjustment hand wheel II through jackscrews.

Further, the zoom lens observation device comprises a zoom lens support device connected to the top of the XYZ three-axis moving platform, a zoom lens is arranged above the zoom lens support device, a magnification fine adjustment knob capable of randomly adjusting the magnification is arranged on the zoom lens, the tail end of the zoom lens is connected with an electronic eyepiece I, and the electronic eyepiece I is connected with a computer end through a USB connecting wire.

Further, the electron light source subassembly includes the light stand, light stand bottom is connected with XYZ triaxial moving platform's top two, light stand right side vertical direction installs the side light source, hold device one interference connection with the light filter on the side light source, light filter holds device one and is fixed with blue light filter one through the semicircle notch that is equipped with, light stand top horizontal direction installs the top light source, top light source holds device two interference connection with the light filter, light filter holds device two and is fixed with blue light filter two through the semicircle notch that is equipped with, light brightness adjustment dish is installed to light stand one end, the lamp switch knob is installed to the other end of light stand, the top of light stand is connected with the light upper cover through the bolt.

Further, the microscopic examination group observation device comprises a microscopic examination group base connected to the three tops of the XYZ three-axis moving platform, the left side of the microscopic examination group base is connected with an objective lens converter through threads, the objective lens converter is connected with a flat field achromatic objective lens on the left side through threads, the right side of the microscopic examination group base is connected with an eyepiece base through a fixing threaded hole, a lens cone is mounted on the eyepiece base, an electronic eyepiece II is mounted above the lens cone, and the electronic eyepiece II is connected with a computer end through a USB connecting wire.

Further, the objective table subassembly includes the stand, the stand passes through the bolt and is fixed with aluminum alloy 6061 bottom plate, stand top joint objective table, be equipped with 45 slopes on the objective table, utilize this slope to be connected with 45 prisms, be equipped with four 1mm high archs on the objective table, long glass groove is arranged in the arch.

Further, the lower cover body of the platform protection cover comprises a lower cover body side plate, a lower cover body left side top plate is clamped above the lower cover body side plate through a peripheral groove, a storage table is clamped on the lower cover body left side top plate through a groove, and a lower cover body right side top plate is clamped above the lower cover body side plate through a peripheral groove.

Further, the upper cover body of the platform protection cover comprises an upper cover body side plate, and an upper cover body top plate is clamped on the upper cover body side plate through a peripheral groove.

Advantageous effects

The biological microscope for synchronously observing the positive and bottom views of living single cells can fix the position and the angle of a sample in the observation process by combining the electronic light source assembly, the microscopic examination group observation device and the magnification-varying lens observation device with the XYZ three-axis moving platform, ensures the observation stability, simultaneously utilizes the rotary rod, the bevel gear and the scale fine adjustment hand wheel to conduct torque reversing, converts the torque of an X axis into a Y axis direction, and simultaneously leads out the XYZ three-axis measuring head outside the protective cover, so that the operation of a user is convenient.

The biological microscope for synchronously observing the positive and bottom views of the living single cells can randomly switch the magnification factors of the designed zoom lens, and compared with the traditional objective lens, the zoom lens has the advantages that the magnification factors of the objective lens are not required to be frequently replaced, the operation flow is simplified, and the operation efficiency and the use experience of users are improved.

The biological microscope for synchronously observing the front and bottom views of living single cells utilizes the 45-degree prism to reflect the vertical bottom view of the sample to the zoom lens which is horizontally arranged, so that the position distribution of the electronic light source assembly, the microscopic group observation device and the zoom lens observation device is more reasonable.

The biological microscope for synchronously observing the front and bottom views of the living single cells synchronously observes the front and bottom views of the living single cells through the cooperation of the microscopic examination group observation device, the zoom lens observation device and each structure, and the three-dimensional geometric dimensions of the length, the width and the thickness can be obtained by quickly extracting the observed front and bottom views on a computer by using software.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model.

Fig. 2 is a schematic bottom structure of the structural bearing seat of the present utility model.

Fig. 3 is a schematic placement diagram of a triaxial moving platform one, a triaxial moving platform two and a triaxial moving platform three according to the present utility model.

Fig. 4 is a schematic diagram of the structure of the zoom lens observation device.

Fig. 5 is a schematic view of an electronic light source assembly according to the present utility model.

FIG. 6 is a schematic view of a stage assembly according to the present utility model.

FIG. 7 is a schematic diagram of the structure of the observation device for the structural microscopic examination set of the present utility model.

FIG. 8 is a schematic view of the structure of the platform protection cover of the present utility model.

FIG. 9 is a schematic view of the upper cover of the platform protector.

FIG. 10 is a schematic view of the lower cover of the platform protection cover according to the present utility model.

In the figure: 1. a bearing seat; 1-1, an aluminum alloy 6061 bottom plate; 1-2, conical foot pads; 2. XYZ three-axis moving platform I; 2-1, bevel gears I; 2-2, "L" baffle I; 2-3, a rotary rod 1;2-4, X-axis measuring head; 2-5, a Z-axis measuring head; 2-6, a scale fine adjustment hand wheel I; 2-7, a scale fine adjustment hand wheel II; 2-8, a scale fine adjustment hand wheel III; 2-9, a rotary rod 2;2-10, a rotary rod 3;2-11, Y-axis measuring head; 2-12 parts of a rotary rod 4; 3. XYZ three-axis moving platform II; 3-1, X-axis measuring head; 3-2, a rotary rod 5;3-3, measuring head of Z axis; 3-4, a rotary rod 6;3-5, measuring head of Y axis; 3-6, bevel gears II; 3-7, a scale fine adjustment hand wheel; 3-8, an L-shaped baffle II; 3-9, a rotary rod 7;3-10, a scale fine adjustment hand wheel; 3-11, a rotary rod 8;3-12, a scale fine adjustment hand wheel is six; 4. a zoom lens observation device; 4-1, electronic eyepiece I; 4-2, zooming lens; 4-3, a zoom lens supporting device; 4-4, multiplying power fine tuning knob; 5. an electronic light source assembly; 5-1, a blue filter I; 5-2, side Fang Guangyuan; 5-3, a first filter holding device; 5-4, lamplight stand columns; 5-5, a lamp brightness adjusting disc; 5-6, lamplight upper cover; 5-7, a lamp switch knob; 5-8, upper light source; 5-9, a second filter holding device; 5-10, a blue filter II; 6. a stage assembly; 6-1, a long glass groove; 6-2, 45 DEG prism; 6-3, an objective table; 6-4, stand columns; 7. an observation device of the microscopic examination group; 7-1, a flat field achromatic objective lens; 7-2, an objective lens converter; 7-3, fixing the threaded holes; 7-4 electronic eyepiece II; 7-5, lens cone; 7-6, an eyepiece base; 7-7, a base of the microscopic examination group; 8. three XYZ three-axis moving platforms; 8-1, an X-axis measuring head; 8-2, a rotary rod 9;8-3, measuring head of Z axis; 8-4, a rotary rod 10;8-5, fine-tuning a hand wheel with scales; 8-6, a Y-axis measuring head; 8-7, a rotary rod 11;8-8, a scale fine adjustment hand wheel eight; 8-9, a scale fine adjustment hand wheel is nine; 8-10, a rotary rod 12;8-11, an L-shaped baffle III; 8-12, bevel gears III; 9. a lower cover body of the platform protection cover; 9-1, lower cover side plates; 9-2, a top plate at the left side of the lower cover body; 9-3, a storage table; 9-4, a top plate on the right side of the lower cover body; 10. the upper cover body of the platform protection cover; 10-1, upper cover side plates; 10-2, upper cover top plate.

Description of the embodiments

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more clear, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-10, a biological microscope for synchronously observing the front and bottom views of living single cells comprises a bearing seat 1, wherein the left side above the bearing seat 1 is connected with an XYZ three-axis moving platform one 2 through a bolt, the top of the XYZ three-axis moving platform one 2 is connected with a zoom lens observation device 4 through a bolt, the front side above the bearing seat 1 is connected with an XYZ three-axis moving platform two 3 through a bolt, the top of the XYZ three-axis moving platform two 3 is connected with an electronic light source component 5 through a bolt, the rear side above the bearing seat 1 is connected with an XYZ three-axis moving platform 8 through a bolt, the top of the XYZ three-axis moving platform 8 is connected with a microscopic observation device 7 through a bolt, the middle part above the bearing seat 1 is connected with a stage component 6 through a bolt, four vertex angles above the bearing seat 1 are connected with a platform protection cover lower cover body 9, and the upper cover body 10 of the platform protection cover lower cover body 9 is clamped through a groove. Through the design of bearing seat 1, the effect of bearing and fixing is played to XYZ triaxial moving platform I2, XYZ triaxial moving platform II 3, XYZ triaxial moving platform III 8, objective table subassembly 6 and platform safety cover, has improved the stability of biological microscope overall structure that synchronous observation living body single cell was just, from bottom.

Wherein, the bearing seat 1 comprises an aluminum alloy 6061 bottom plate 1-1, and the aluminum alloy 6061 bottom plate 1-1 is connected with four conical foot pads 1-2 through bolts. The four conical foot pads 1-2 are distributed on four corners of the aluminum alloy 6061 bottom plate 1-1, the four conical foot pads 1-2 can increase the stability of the aluminum alloy 6061 bottom plate 1-1, and meanwhile friction and abrasion between the aluminum alloy 6061 bottom plate 1-1 and a contact surface can be reduced.

The first XYZ three-axis moving platform 2 is connected with the aluminum alloy 6061 base plate 1-1 through bolts, the first XYZ three-axis moving platform 2 is provided with an X-axis measuring head 2-4, the X-axis measuring head 2-4 is connected with a rotary rod 1 2-3 through jackscrews, the rotary rod 1 2-3 is connected with the first bevel gear 2-1 through jackscrews, two bevel gears are meshed, the other bevel gear is connected with the rotary rod 4 2-12 through jackscrews, the rotary rod 4 2-12 is connected with the third scale fine adjustment hand wheel 2-8 through jackscrews, the first XYZ three-axis moving platform 2 is provided with a Y-axis measuring head 2-11, the Y-axis measuring head 2-11 is connected with the rotary rod 3 2-10 through jackscrews, the rotary rod 3 2-10 is connected with the second scale fine adjustment hand wheel 2-7 through jackscrews, the first XYZ three-axis moving platform 2-5 is connected with the rotary rod 2 2-9 through jackscrews, and the second XYZ-axis measuring head 2-5 is connected with the first scale fine adjustment hand wheel 2-6 through jackscrews. The first XYZ three-axis moving platform 2 is used for bearing the upper zoom lens observation device 4, and the positions of the upper zoom lens observation device 4 are adjusted from the directions X, Y and Z by adjusting the first scale fine adjustment hand wheel 2-6, the second scale fine adjustment hand wheel 2-7 and the third scale fine adjustment hand wheel 2-8 on the first XYZ three-axis moving platform 2 so as to realize fine adjustment of the focus position of the living single cell by the zoom lens observation device 4. The XYZ three-axis moving platform II 3 is connected with the aluminum alloy 6061 base plate 1-1 through bolts, the X-axis measuring head 3-1 is installed on the XYZ three-axis moving platform II 3, the X-axis measuring head 3-1 is connected with the rotary rod 5 3-2 through jackscrews, the rotary rod 5 3-2 is connected with the bevel gear II 3-6 through jackscrews, two bevel gears are meshed, the other bevel gear is connected with the rotary rod 8 3-11 through jackscrews, the rotary rod 3-11 is connected with the scale fine adjustment hand wheel six 3-12 through jackscrews, the Y-axis measuring head 3-5 is installed on the XYZ three-axis moving platform II 3, the Y-axis measuring head 3-5 is connected with the rotary rod 7 3-9 through jackscrews, the rotary rod 7 3-9 is connected with the scale fine adjustment hand wheel five 3-10 through jackscrews, the Z-axis measuring head 3-3 is installed on the XYZ three-axis moving platform II 3, the Z-axis measuring head 3-3 is connected with the rotary rod 6 3-4 through jackscrews, and the rotary rod 6 3-4 is connected with the scale fine adjustment hand wheel four-7 through jackscrews. The second XYZ three-axis moving platform 3 is used for bearing the electronic light source assembly 5 thereon, and the positions of the electronic light source assembly 5 thereon are adjusted from the directions X, Y and Z by adjusting the fourth 3-7 scale fine-tuning hand wheel, the fifth 3-10 scale fine-tuning hand wheel and the sixth 3-12 scale fine-tuning hand wheel on the second XYZ three-axis moving platform 3 so as to realize fine adjustment of the focusing position of the living single cell by the electronic light source assembly 5. The three-8 XYZ three-axis moving platform is connected with the aluminum alloy 6061 base plate 1-1 through bolts, the three-8 XYZ three-axis moving platform is provided with the X-axis measuring head 8-1, the X-axis measuring head 8-1 is connected with the rotary rod 9 8-2 through jackscrews, the rotary rod 9 8-2 is connected with the three bevel gears 8-12 through jackscrews, two bevel gears are meshed, the other bevel gear is connected with the rotary rod 12-10 through jackscrews, the rotary rod 12-10 is connected with the scale fine adjustment hand wheel nine 8-9 through jackscrews, the three-8 XYZ three-axis moving platform is provided with the Y-axis measuring head 8-6, the Y-axis measuring head 8-6 is connected with the rotary rod 11-7 through jackscrews, the rotary rod 11-7 is connected with the scale fine adjustment hand wheel eight 8-8 through jackscrews, the three-8 XYZ three-axis moving platform is provided with the Z-axis measuring head 8-3 is connected with the rotary rod 10-4 through jackscrews, and the rotary rod 10-4 is connected with the scale fine adjustment hand wheel seven 8-5 through jackscrews. The XYZ three-axis moving platform III 8 is used for bearing the upper microscopic examination group observation device 7, and the positions of the upper microscopic examination group observation device 7 are adjusted from the directions X, Y and Z by adjusting the scale fine adjustment hand wheel seven 8-5, the scale fine adjustment hand wheel eight 8-8 and the scale fine adjustment hand wheel nine 8-9 on the XYZ three-axis moving platform III 8 so as to realize fine adjustment of the focal position of the living single cell by the microscopic examination group observation device 7.

The zoom lens observation device 4 comprises a zoom lens support device 4-3 which is connected to the top of an XYZ three-axis moving platform I2 through bolts, a zoom lens 4-2 is arranged above the zoom lens support device 4-3, a magnification fine adjustment knob 4-4 which can randomly adjust the magnification is arranged on the zoom lens 4-2, the tail end of the zoom lens 4-2 is connected with an electronic eyepiece I4-1 through threads, the electronic eyepiece I4-1 is connected with a computer end through a USB connecting wire, fine focusing is performed through adjusting the XYZ three-axis moving platform I2, and a bottom view of a living single cell can be captured on the computer.

The electronic light source assembly 5 comprises a light stand column 5-4, the bottom of the light stand column 5-4 is connected with the top of a XYZ triaxial moving platform II 3 through a bolt, a lateral light source 5-2 is arranged in the right vertical direction of the light stand column 5-4 through threads, the lateral light source 5-2 is in interference connection with a light filter accommodating device I5-3, the light filter accommodating device I5-3 is fixed with a blue light filter I5-1 through a semicircular notch arranged on the light filter accommodating device I, an upper light source 5-8 is arranged in the upper horizontal direction of the light stand column 5-4 through threads, the upper light source 5-8 is in interference connection with a light filter accommodating device II 5-9, the light filter accommodating device II 5-9 is fixed with a blue light filter II 5-10 through a semicircular notch arranged on the light stand column 5-4, a light brightness adjusting disc 5-5 is arranged at one end of the light stand column 5-4 through a bolt, and a light switch knob 5-7 is arranged at the other end of the light stand column 5-4 through a bolt. The light rays of the two light sources are concentrated on the living single cell by adjusting the XYZ three-axis moving platform II 3. The electronic light source assembly 5 can provide different brightness selections of three kinds of lamplight, and provides good illumination conditions for the microscopic platform. The blue filter can make the details of the edge part of the living single cell clearer, and can improve the imaging quality.

The microscopic examination group observation device 7 comprises a microscopic examination group base 7-7 which is connected to the top of a XYZ triaxial moving platform III 8 through a bolt, the left side of the microscopic examination group base 7-7 is connected with an objective lens converter 7-2 through threads, the objective lens converter 7-2 is connected with a flat field achromatic objective lens 7-1 on the left side through threads, the right side of the microscopic examination group base 7-7 is connected with an eyepiece base 7-6 through a fixed threaded hole 7-3, a lens cone 7-5 is mounted on the eyepiece base 7-6, an electronic eyepiece II 7-4 is mounted above the lens cone 7-5, and the electronic eyepiece II 7-4 is connected with a computer end through a USB connecting wire. By adjusting the XYZ three-axis moving platform III 8 to carry out fine focusing, the front view of the living single cell can be captured on a computer.

The objective table component 6 comprises an upright post 6-4, the upright post 6-4 is fixed with an aluminum alloy 6061 bottom plate 1-1 through bolts, the top of the upright post 6-4 is clamped with an objective table 6-3, a 45-degree slope is arranged on the objective table 6-3, the objective table 6-3 is bonded with a 45-degree prism 6-2 through the slope, four bulges with the height of 1mm are arranged on the objective table 6-3, and a long glass groove 6-1 is positioned in the bulges. In the test, a long glass groove 6-1 containing the living single cell is placed on a 45-degree prism 6-2, and the first 2, second 3 and third 8 XYZ three-axis moving platforms are adjusted to perform positioning and focusing so as to obtain the front and bottom views of the living single cell. The object stage 6-3 and the upright posts 6-4 play a role in supporting and stabilizing the long glass groove 6-1 and the 45-degree prism 6-2.

The lower cover body 9 of the platform protection cover comprises a lower cover body side plate 9-1, a lower cover body left side top plate 9-2 is clamped above the lower cover body side plate 9-1 through a peripheral groove, a storage table 9-3 is clamped on the lower cover body left side top plate 9-2 through a groove, and a lower cover body right side top plate 9-4 is clamped above the lower cover body side plate 9-1 through a peripheral groove. The design of the left top plate 9-2 of the lower cover body and the right top plate 9-4 of the lower cover body can meet the requirement that the triaxial moving platform moves in X, Y and Z directions, and the side wall of the side plate 9-1 of the lower cover body is provided with small holes which can lead out scale fine-tuning hand wheels.

The upper cover body 10 of the platform protection cover comprises an upper cover body side plate 10-1, and the upper cover body side plate 10-1 is clamped with an upper cover body top plate 10-2 through a peripheral groove. The upper cover body 10 of the protective cover plays roles of dust prevention and lens protection.

Working principle: when in use, the biological microscope is placed on a horizontal stable working plane, a power supply is connected through an electric socket, an extracted living single cell sample is placed in a clean long glass groove 6-1, the long glass groove 6-1 is clamped in four 1mm bulges of an objective table 6-3, and a 45-degree prism 6-2 is bonded on a 45-degree slope on the objective table 6-3. The method comprises the steps of selecting a proper flat-field achromatic objective 7-1, adjusting a scale fine adjustment hand wheel four 3-7, a scale fine adjustment hand wheel five 3-10 and a scale fine adjustment hand wheel six 3-12 on a XYZ triaxial moving platform II 3, enabling light rays of a side light source 5-2 and an upper light source 5-8 on an electronic light source assembly 5 to be gathered on living single cells in a long glass groove 6-1, adjusting a scale fine adjustment hand wheel seven 8-5, a scale fine adjustment hand wheel eight 8-8 and a scale fine adjustment hand wheel nine 8-9 on a XYZ triaxial moving platform III 8, controlling the flat-field achromatic objective 7-1 to move to the vicinity of a sample long glass groove 6-1, until the form of a living single cell sample in the long glass groove 6-1 can be clearly seen, guiding a front view of a lens observation device 7 to a living single cell to be stored in a computer through an electronic eyepiece II 7-4 after adjustment, adjusting the scale fine adjustment hand wheel II 2-7 and the scale fine adjustment hand wheel II-7-8 on the XYZ triaxial moving platform II, controlling a variable lens 4-2 to move to the vicinity of the scale fine adjustment hand wheel 8-9, enabling the lens II-7-4 to be guided to a lens 5-45 DEG to a proper brightness adjustment device to be turned to the electronic lens 4 until the form of the sample can be seen in the lens is clear, and the lens is adjusted to the lens 4-5-45-a proper brightness adjustment device can be seen to the single cell. By the method, the front view and the bottom view of the living single cell can be synchronously observed, and the software can be used for acquiring three-dimensional geometric information such as the length, the width, the thickness and the like of the living single cell from the two views.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the spirit and scope of the utility model as defined by the appended claims and their equivalents.

Claims (6)

1. The utility model provides a biological microscope of synchronous observation living body single cell normal, bottom view, includes bearing seat (1), its characterized in that: the device is characterized in that an XYZ three-axis moving platform I (2) is connected to the left side above the bearing seat (1), a zoom lens observation device (4) is connected to the top of the XYZ three-axis moving platform I (2), an XYZ three-axis moving platform II (3) is connected to the front side above the bearing seat (1), an electronic light source component (5) is connected to the top of the XYZ three-axis moving platform II (3), an XYZ three-axis moving platform III (8) is connected to the rear side above the bearing seat (1), a lens inspection group observation device (7) is connected to the top of the XYZ three-axis moving platform III (8), and an objective table component (6) is connected to the middle part above the bearing seat (1).

2. A biological microscope for simultaneously observing the front and bottom views of a living single cell according to claim 1, wherein: the bearing seat (1) comprises an aluminum alloy 6061 bottom plate (1-1), and the aluminum alloy 6061 bottom plate (1-1) is connected with four conical foot pads (1-2) through bolts.

3. A biological microscope for simultaneously observing the front and bottom views of a living single cell according to claim 1, wherein: the zoom lens observation device (4) comprises a zoom lens support device (4-3) connected to the top of an XYZ triaxial mobile platform I (2), a zoom lens (4-2) is arranged above the zoom lens support device (4-3), a magnification fine adjustment knob (4-4) capable of randomly adjusting the magnification is arranged on the zoom lens (4-2), the tail end of the zoom lens (4-2) is connected with an electronic eyepiece I (4-1), and the electronic eyepiece I (4-1) is connected with a computer end through a USB connecting wire.

4. A biological microscope for simultaneously observing the front and bottom views of a living single cell according to claim 1, wherein: the electronic light source assembly (5) comprises a light stand column (5-4), the bottom of the light stand column (5-4) is connected with the top of a XYZ triaxial mobile platform II (3), a side Fang Guangyuan (5-2) is arranged on the right side of the light stand column (5-4) in the vertical direction, the side Fang Guangyuan (5-2) is in interference connection with a light filter accommodating device I (5-3), the light filter accommodating device I (5-3) is fixed with a blue light filter I (5-1) through a semicircular notch arranged on the light stand column, an upper light source (5-8) is arranged above the light stand column (5-4) in the horizontal direction, the upper light source (5-8) is in interference connection with a light filter accommodating device II (5-9), the light filter accommodating device II (5-9) is fixed with the blue light filter II (5-10) through the semicircular notch arranged on the light stand column (5-4), a light brightness adjusting disc (5-5) is arranged at one end of the light stand column, a light switch knob (5-7) is arranged at the other end of the light stand column (5-4), and a light switch (5-4) is connected with a light cover (5-6) through a light bolt.

5. A biological microscope for simultaneously observing the front and bottom views of a living single cell according to claim 1, wherein: the microscopic examination group observation device (7) comprises a microscopic examination group base (7-7) connected to the top of a three (8) XYZ triaxial moving platform, the left side of the microscopic examination group base (7-7) is connected with an objective lens converter (7-2) through threads, the objective lens converter (7-2) is connected with a flat field achromatic objective lens (7-1) on the left side through threads, the right side of the microscopic examination group base (7-7) is connected with an eyepiece base (7-6) through a fixed threaded hole (7-3), a lens cone (7-5) is mounted on the eyepiece base (7-6), an electronic eyepiece second (7-4) is mounted above the lens cone (7-5), and the electronic eyepiece second (7-4) is connected with a computer end through a USB connecting wire.

6. A biological microscope for simultaneously observing the front and bottom views of a living single cell according to claim 1, wherein: the objective table assembly (6) comprises an upright post (6-4), the upright post (6-4) is fixed with an aluminum alloy 6061 base plate (1-1) through a bolt, an objective table (6-3) is clamped at the top of the upright post (6-4), a 45-degree slope is arranged on the objective table (6-3), the objective table is bonded with a 45-degree prism (6-2) through the slope, four bulges with the height of 1mm are arranged on the objective table (6-3), and a long glass groove (6-1) is arranged in the bulges.