CN220201915U

CN220201915U - Protein glutaminase preparation facilities

Info

Publication number: CN220201915U
Application number: CN202320587490.4U
Authority: CN
Inventors: 俞宙汝; 李立; 葛春妹
Original assignee: Shanghai Qingmei Food Co ltd
Current assignee: Shanghai Qingmei Food Co ltd
Priority date: 2023-03-23
Filing date: 2023-03-23
Publication date: 2023-12-19
Anticipated expiration: 2033-03-23

Abstract

The utility model relates to the technical field of food microorganism production, and discloses a centrifugal mechanism which is characterized in that clear water is injected into an inner tank body from a water pipe, a motor is kept to work, an electromagnetic valve is kept to be opened, the clear water is promoted to rapidly clean the inner tank body and an outer tank body, the cleaned water is discharged outwards from a discharge pipe and a discharge hole, equipment can be rapidly cleaned without disassembly, the cleanliness of recycling is ensured, the filtering effect is improved, the manual cleaning load is reduced, the centrifugal mechanism comprises a filter membrane, and the filter membrane is fixedly arranged on the inner wall of the inner tank body. Through annotating the internal with the clear water from the water pipe into inner tank, keep the motor work, keep the solenoid valve open, make clear water rinse inner tank body and outer jar body fast to make the outside emission of clear water liquid follow discharging pipe and discharge gate after wasing, need not dismantle can carry out cleaning treatment with equipment fast, guarantee the cleanliness of cyclic utilization, improve the filter effect, reduce artifical clean burden.

Description

Protein glutaminase preparation facilities

Technical Field

The utility model relates to the technical field of food microorganism production, in particular to a protein glutaminase preparation device.

Background

The glutamine transaminase is also called transglutaminase, and is a monomer protein with an active center and composed of 331 amino groups and having a molecular weight of about 38000, and the monomer protein can catalyze covalent crosslinking of protein polypeptides in molecules and between molecules, so that the structure and the function of the protein are improved, and the properties of the protein are as follows: the foaming property, the emulsifying stability, the heat stability, the water retention property, the gel capability and the like have remarkable effects, so that the flavor, the taste, the texture, the appearance and the like of the food are improved.

According to Chinese patent publication No. CN107325977B, the patent provides a method for producing protein glutaminase, purification and application, and the method comprises the following steps: ultrafiltering and concentrating, centrifuging the fermentation liquor to remove thalli, filtering the supernatant with a 0.22 μm filter membrane in a filter press to remove insoluble impurities, concentrating by an ultrafiltration system, and performing the second step: precipitating the ethanol precipitation concentrate by absolute ethanol, standing, centrifuging to obtain protein precipitate, re-dissolving the protein precipitate by ddH2O, centrifuging again to remove insoluble impurities to obtain a crude protein glutaminase extract, and a third step: desalting, filtering the crude extract with 0.45 μm filter membrane, and desalting with desalting column; collecting the protein component at a position having an absorption peak at 280 nm; ion exchange chromatography is carried out by a cation exchange chromatography column, and the fourth step: cation exchange, the protein component collected by desalination is subjected to cation exchange chromatography by a cation exchange chromatography column; collecting the components at the position with an absorption peak at 280nm, and a fifth step: concentrating, namely dialyzing and concentrating the components collected by cation exchange by using PEG 20000 to obtain a concentrated solution containing the enzyme activity, and a sixth step: gel filtration chromatography, filtering with 0.22 μm filter membrane, and collecting components at 280nm with absorption peak.

But fermentation broth and concentrate all need carry out centrifugal treatment, lead to centrifugal equipment to need the first time clean in order to link up fast after using and throw into next process, can involve dismantling and installation change centrifugal equipment's clean process, have increased manual operation burden, are difficult to realize reasonable collocation between the process.

Disclosure of Invention

(one) solving the technical problems

Aiming at the defects of the prior art, the utility model provides a protein glutaminase preparation device. The automatic cleaning device has the advantages that clear water can be injected into the inner tank body from the water pipe, the motor is kept to work, the electromagnetic valve is kept to be opened, the inner tank body and the outer tank body are quickly cleaned by the clear water, the cleaned water is discharged outwards from the discharging pipe and the discharging hole, and therefore equipment can be quickly cleaned without being disassembled, and the manual cleaning burden is reduced.

(II) technical scheme

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a protein glutaminase preparation facilities, includes outer jar body and inner tank body, be provided with centrifugal mechanism on the inner tank body, be provided with clean mechanism on the outer tank body, still be provided with control assembly on the outer tank body, and control assembly includes L arm, motor, pivot, spur gear, the bottom opening setting of the outer tank body, and the opening part intercommunication is provided with the discharge gate, the one end of the outer wall fixedly connected with L board of the outer tank body.

Preferably, the centrifugal mechanism comprises a filter membrane, the filter membrane is fixedly mounted on the inner wall of the inner tank body, a plurality of liquid outlet holes are formed in the outer wall of the inner tank body in a surrounding mode, an outer toothed ring is fixedly sleeved on the outer wall of the inner tank body, one end of a rotary joint is arranged at the bottom of the inner tank body in a communicating mode, one end of a discharging pipe is arranged at the other end of the rotary joint in a communicating mode, connecting rods are fixedly mounted on two sides of the outer wall of the other end of the discharging pipe, and an electromagnetic valve is fixedly mounted on the discharging pipe. By this preference, the fermentation broth is filtered out of the filter membrane by centrifugal force and falls from the outlet opening into the outer tank.

Preferably, one ends of the connecting rods on the discharging pipes, which are far away from each other, are sequentially and fixedly connected to the inner wall of the bottom opening of the outer tank body. Through this preference, the connecting rod secures the tapping pipe and provides support for the inner vessel.

Preferably, the cleaning mechanism comprises a support plate, the support plate is fixedly arranged at the top of the outer wall of the outer tank body, an upper disc is fixedly arranged on one side, far away from the outer tank body, of the support plate, two electric push rods are sequentially and fixedly arranged at the top of the upper disc, inner rods of the two electric push rods movably penetrate through the upper disc and extend downwards, a lower disc is fixedly arranged at the extending ends of the two electric push rods, and one end of a water pipe is arranged at the center of the top of the lower disc in a communicating mode. Through this preference, the lower wall laminating inner tank body jar mouth avoids centrifugal rotatory in liquid spill.

Preferably, the sliding hole is formed in the center of the top of the upper disc, the other end of the water pipe is connected with the inner wall of the sliding hole in a sliding mode and extends upwards, and the bottom of the lower disc is attached to the outer wall of the tank opening of the inner tank body. Through this preference, the water pipe passes through the slide hole through the lift of electric push rod drive and can not block.

Preferably, one end fixed connection of L arm is at the outer wall of outer jar body, motor fixed mounting is at the other end top of L arm, the output of motor passes through the one end fixed connection of shaft coupling and pivot, the other end activity of pivot runs through L arm and downwardly extending, the fixed cover of spur gear is established at the extension end outer wall of pivot to with the ring gear engagement. Through this preference, the starter motor makes the pivot drive the spur gear rotation to drive the rotation of inner tank body through meshing outer ring gear, filter the fermentation broth out of filter membrane through centrifugal force.

(III) beneficial effects

Compared with the prior art, the utility model provides a protein glutaminase preparation device, which has the following beneficial effects:

1. according to the protein glutaminase preparation device, clear water is injected into the inner tank body from the water pipe, the motor is kept to work, the electromagnetic valve is kept to be opened, the clear water is enabled to rapidly clean the inner tank body and the outer tank body, the cleaned water is enabled to be discharged outwards from the discharging pipe and the discharging hole, equipment can be rapidly cleaned without disassembling, the cleanliness of recycling is guaranteed, the filtering effect is improved, and the manual cleaning burden is reduced.

2. According to the protein glutaminase preparation device, the rotating shaft drives the spur gear to rotate through the starting motor, the outer toothed ring is meshed to drive the inner tank body to rotate, fermentation liquor is filtered out of the filter membrane discharge port through centrifugal force to slide and be recovered, the electromagnetic valve is opened, the fermentation liquor filtered in the inner tank body flows into a new container to be recovered, the liquid filtered by the centrifugal force is rapidly classified and recovered, and the recovery efficiency is improved.

Drawings

The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the embodiments of the utility model, serve to explain the utility model. In the drawings:

FIG. 1 is a perspective view of the present utility model;

FIG. 2 is a cross-sectional elevation view of the present utility model;

fig. 3 is a cross-sectional top view of the outer can of the present utility model.

In the figure: 1. an outer can; 2. an inner tank; 3. a centrifugal mechanism; 301. a filter membrane; 302. an outer toothed ring; 303. a rotary joint; 304. a discharge pipe; 305. a connecting rod; 306. an electromagnetic valve; 4. a cleaning mechanism; 401. a support plate; 402. a top plate; 403. an electric push rod; 404. a lower plate; 405. a water pipe; 5. an L arm; 6. a motor; 7. a rotating shaft; 8. spur gears; 9. a discharge port; 10. l plate.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.

Example 1

As shown in fig. 1-3, the utility model provides a protein glutaminase preparation device, which comprises an outer tank 1 and an inner tank 2, wherein a centrifugal mechanism 3 is arranged on the inner tank 2, a cleaning mechanism 4 is arranged on the outer tank 1, a control component is further arranged on the outer tank 1, the control component comprises an L arm 5, a motor 6, a rotating shaft 7 and a spur gear 8, the bottom opening of the outer tank 1 is arranged, the opening is communicated with a discharge hole 9, the outer wall of the outer tank 1 is fixedly connected with one end of an L plate 10, the centrifugal mechanism 3 comprises a filter membrane 301, the filter membrane 301 is fixedly arranged on the inner wall of the inner tank 2, a plurality of liquid outlet holes are formed in the outer wall of the inner tank 2 in a surrounding manner, an outer toothed ring 302 is fixedly sleeved on the outer wall of the inner tank 2, one end of the inner tank 2 is communicated with a rotary joint 303, one end of the rotary joint 303 is communicated with the other end of the rotary joint 303, two sides of the outer wall of the other end of the rotary joint 304 are fixedly provided with a connecting rod 305, an electromagnetic valve 306 is fixedly arranged on the discharge pipe 304, the centrifugal force filters fermentation liquor out of the filter membrane 301, and the filter membrane 304 falls into the inner wall of the inner tank 2 from the inner tank 2, the inner tank 2 is fixedly connected with the connecting rod 305, and the inner tank 2 is sequentially far from the inner tank 2, and the inner tank 2 is fixedly connected with the inner tank 2, and the inner tank is sequentially far from the discharge hole of the inner tank 2.

In this embodiment, through annotating the clear water in the inner tank body 2 from water pipe 405, keep motor 6 work, keep solenoid valve 306 open, make clear water rinse inner tank body 2 and outer tank body 1 fast to make the water liquid after rinsing outwards discharge from discharging pipe 304 and discharge gate 9, need not dismantle can carry out cleaning treatment with equipment fast, guarantee the cleanliness of cyclic utilization, improve the filter effect, reduce artifical clean burden.

Example two

As shown in fig. 1-3, on the basis of the first embodiment, the present utility model provides a technical solution: preferably, the cleaning mechanism 4 includes the extension board 401, extension board 401 fixed mounting is at the outer wall top of outer jar body 1, one side fixed mounting that outer jar body 1 was kept away from to the extension board 401 has upper disc 402, the top of upper disc 402 is fixed mounting in proper order has two electric putter 403, the interior pole of two electric putter 403 all activity is passed through upper disc 402 and is extended downwards, and fixed mounting has lower disc 404 on the extension end of two electric putter 403, the top center intercommunication of lower disc 404 is provided with the one end of water pipe 405, the one end of lower disc 404 laminating inner jar body 2 jar mouth, avoid liquid spill in the centrifugal rotation, the slide opening has been seted up at the top center of upper disc 402, the other end and the inner wall sliding connection of slide opening of water pipe 405, and upwards extend, the bottom of lower disc 404 and the outer wall laminating of jar mouth of inner jar body 2, water pipe 405 drives through electric putter 403 and goes up and down through the slide opening in can not the card to die, the one end fixed connection of L arm 5 is at the outer wall of outer jar body 1, the other end top of L arm 5 is fixed mounting at L arm 5, the motor 6, the output of motor 6 passes through the fixed connection of one end 7 and 7, the other end of 7 and 7 is set up at the fixed connection of L arm 7 and the fixed connection of outer end 7 and the fixed connection of outer jar 7, the rotation 8 is passed through the fixed ring of rotation 8 and the outer tooth-shaped filter membrane is meshed with the outer jar 302, thereby the outer rotary ring is driven to rotate the outer rotary ring 2, the outer rotary ring is meshed with the rotary filter membrane is driven, the rotary filter membrane is meshed with the rotary outer rotary ring is 8, and the rotary filter membrane is meshed with the rotary filter membrane is 2, and the filter membrane is meshed with the filter 2.

In this embodiment, the rotating shaft 7 drives the spur gear 8 to rotate by starting the motor 6, and engages the outer toothed ring 302 to drive the inner tank 2 to rotate, the fermentation liquor is filtered out of the filter membrane 301 through centrifugal force, the discharge port 9 slides to be recovered, the electromagnetic valve 306 is opened to enable the fermentation liquor filtered in the inner tank 2 to flow into a new container to be recovered, the liquid filtered by centrifugal force is quickly classified and recovered, and the recovery efficiency is improved.

The working principle of the protein glutaminase preparation device is specifically described below, when the protein glutaminase preparation device is used, the L plate 10 is used for supporting the outer tank body 1, the container for containing is placed on the L plate 10, the discharge hole 9 is aligned to the solution after Cheng Jieli heart filtration, so that the preparation process of the protein glutaminase is better connected, the inner rod of the electric push rod 403 is contracted, the lower plate 404 is driven to be far away from the tank hole of the inner tank body 2, fermentation liquor is poured into the inner tank body 2, the inner rod of the electric push rod 403 is then driven to push the lower plate 404 to be attached to the tank hole of the inner tank body 2, liquid in centrifugal rotation is prevented from being spilled, the motor 6 is started to drive the spur gear 8 to rotate, the inner tank body 2 is driven to rotate by meshing the outer toothed ring 302, the fermentation liquor is filtered out of the filter membrane 301 through centrifugal force, the separated fermentation liquor falls into the outer tank body 1 from the discharge hole, the inner wall of the outer tank body 1 slides towards the discharge hole 9 and enters the container for recovery, a new container is replaced after recovery, the electromagnetic valve 306 is opened, the filtered fermentation liquor in the inner tank body 2 flows into the inner tank body 2, the electromagnetic valve 306 is then is driven to be directly aligned to the discharge pipe 304, the liquid after the recovery is carried out, the electromagnetic valve 306 is prevented from flowing into the inner tank 2, the filter liquor is cleaned, the filter tank 2 is cleaned, the water is cleaned, the filter tank is directly after the filter tank is cleaned, the water is cleaned from the filter tank is cleaned, the water is directly is cleaned, and the water is cleaned from the inner tank 2, the filter tank is cleaned, the filter tank is directly is cleaned by the water from the inner tank is cleaned, and the filter tank is cleaned, and the water is directly is removed from the inner tank is directly, and the filter tank is directly after the filter is washed.

Claims

1. The protein glutaminase preparation device comprises an outer tank body (1) and an inner tank body (2), and is characterized in that: be provided with centrifugal mechanism (3) on inner tank body (2), be provided with clean mechanism (4) on outer tank body (1), still be provided with control assembly on outer tank body (1), and control assembly includes L arm (5), motor (6), pivot (7), spur gear (8), the bottom opening setting of outer tank body (1), and the opening part intercommunication is provided with discharge gate (9), the one end of outer tank body (1) outer wall fixedly connected with L board (10).

2. The protein glutaminase production apparatus according to claim 1, wherein: the centrifugal mechanism (3) comprises a filter membrane (301), the filter membrane (301) is fixedly arranged on the inner wall of the inner tank body (2), a plurality of liquid outlet holes are formed in the outer wall of the inner tank body (2) in a surrounding mode, an outer toothed ring (302) is fixedly sleeved on the outer wall of the inner tank body (2), one end of a rotary joint (303) is arranged at the bottom of the inner tank body (2) in a communicating mode, one end of a discharging pipe (304) is arranged at the other end of the rotary joint (303) in a communicating mode, connecting rods (305) are fixedly arranged on two sides of the outer wall of the other end of the discharging pipe (304), and electromagnetic valves (306) are fixedly arranged on the upper portions of the discharging pipe (304).

3. The protein glutaminase production apparatus according to claim 2, characterized in that: one end of the connecting rod (305) on the discharging pipe (304) which is far away from each other is fixedly connected with the inner wall of the bottom opening of the outer tank body (1) in sequence.

4. A protein glutaminase production apparatus according to claim 3, characterized in that: clean mechanism (4) including extension board (401), extension board (401) fixed mounting is at the outer wall top of outer jar body (1), one side fixed mounting that outer jar body (1) was kept away from to extension board (401) has upper disc (402), the top of upper disc (402) is fixed mounting in proper order has two electric putter (403), two the interior pole of electric putter (403) all activity run through upper disc (402) and downwardly extending, and fixed mounting has lower disc (404) on the extension end of two electric putter (403), the top center intercommunication of lower disc (404) is provided with the one end of water pipe (405).

5. The protein glutaminase production apparatus according to claim 4, wherein: the sliding hole is formed in the center of the top of the upper disc (402), the other end of the water pipe (405) is connected with the inner wall of the sliding hole in a sliding mode and extends upwards, and the bottom of the lower disc (404) is attached to the outer wall of the tank opening of the inner tank body (2).

6. A protein glutaminase production apparatus according to claim 3, characterized in that: one end fixed connection at outer jar outer wall of body (1) of L arm (5), motor (6) fixed mounting is at the other end top of L arm (5), the output of motor (6) passes through the one end fixed connection of shaft coupling and pivot (7), the other end activity of pivot (7) runs through L arm (5) and downwardly extending, spur gear (8) fixed cover is established at the extension end outer wall of pivot (7) to with outer tooth ring (302) meshing.