CN220188323U - Illumination and fluorescence excitation assembly and cell counter - Google Patents

Abstract

The utility model discloses an illumination and fluorescence excitation assembly and a cell counter, wherein the illumination and fluorescence excitation assembly is used in the cell counter and comprises a white light lamp bead and two fluorescence excitation lamp groups, the white light lamp bead is arranged above a cell sample, and a light emitting end of the white light lamp bead is arranged towards the cell sample so that white light emitted by the white light lamp bead irradiates the cell sample; the two fluorescent excitation lamp groups are arranged above the cell sample, the two fluorescent excitation lamp components are arranged on two sides of the white light lamp bead and are oppositely arranged, and the light emitting ends of the two fluorescent excitation lamp groups are respectively arranged towards the cell sample, so that excitation light emitted by the two fluorescent excitation lamp groups can respectively irradiate the cell sample and are used for respectively exciting the cell sample to emit corresponding fluorescence; the illumination and fluorescence excitation assembly is only composed of the white light lamp beads and the two fluorescence excitation lamp groups, so that the structure is simple, the occupied space of the illumination and fluorescence excitation assembly is reduced, and the volume of the cell counter is further reduced.

Description

Illumination and fluorescence excitation assembly and cell counter

Technical Field

The utility model relates to the technical field of cell observation, in particular to an illumination and fluorescence excitation assembly and a cell counter.

Background

In cell biology research, observation and calculation of the number of cell morphology are required. The existing cell image cell identification and counting method is based on a single-view image shot under a microscope, and the existing cell counter is large in size due to the internal structure, so that the cell image cell identification and counting method is inconvenient to carry and move and occupies more space; meanwhile, in the existing cell counter, the structure of a mechanism for collecting the cell image is complex, so that the occupied space of the structure for fluorescence excitation is large, and the design of the cell counter for reducing the volume is difficult to realize.

Disclosure of Invention

The utility model mainly aims to provide an illumination and fluorescence excitation assembly and a cell counter, and aims to solve the problems that the cell counter is large in size due to the fact that a mechanism for acquiring cell images in the existing cell counter is complex in structure and large in occupied space.

To achieve the above object, the present utility model provides an illumination and fluorescence excitation assembly for use in a cytometer, the illumination and fluorescence excitation assembly comprising:

the white light lamp bead is arranged above the cell sample, and the light emitting end of the white light lamp bead is arranged towards the cell sample so that white light emitted by the white light lamp bead irradiates the cell sample; the method comprises the steps of,

the two fluorescent excitation lamp groups are arranged above the cell sample, the two fluorescent excitation lamp components are arranged on two sides of the white light lamp beads and are oppositely arranged, and the light emitting ends of the two fluorescent excitation lamp groups are respectively arranged towards the cell sample, so that excitation light emitted by the two fluorescent excitation lamp groups can respectively irradiate the cell sample and are used for respectively exciting the cell sample to emit corresponding fluorescence.

Optionally, the two fluorescent excitation light groups include a first fluorescent excitation light group and a second fluorescent excitation light group, the first fluorescent excitation light group is formed with a first excitation light irradiation region at a position corresponding to the cell sample, and the second fluorescent excitation light group is formed with a second excitation light irradiation region at a position corresponding to the cell sample;

the first excitation light irradiation area and/or the second excitation light irradiation area are/is overlapped with the white light irradiation area formed by the white light lamp beads.

Optionally, the two fluorescent excitation lamp groups are symmetrically arranged at two sides of the white light lamp bead, an included angle is formed between the central axis of each fluorescent excitation lamp group and the central axis of the white light lamp bead, and the included angle is alpha, wherein alpha is more than 0 degrees and less than 90 degrees.

Optionally, an included angle α=45° between a central axis of each fluorescent-excitation lamp group and a central axis of the white-light lamp bead.

Optionally, each fluorescent excitation lamp group includes an excitation lamp bead, an optical filter and a collimating lens, which are sequentially arranged side by side at intervals, wherein the light-emitting side of the collimating lens is arranged towards the cell sample, so that excitation light emitted from the light-emitting end of the excitation lamp bead sequentially passes through the optical filter for filtering and the collimating lens for collimation and then is emitted to the cell sample;

the light-emitting end of the fluorescent excitation lamp group comprises the light-emitting end of the excitation lamp bead.

Optionally, each fluorescent excitation lamp group further comprises a light-transmitting shell, and a mounting cavity is formed in the light-transmitting shell;

the excitation light beads, the optical filters and the collimating lenses are arranged in the mounting cavity.

Optionally, the collimating lens is configured as a plano-convex lens, a convex side of the plano-convex lens being configured to be disposed toward the cell sample;

wherein the light-emitting side of the collimating lens comprises the convex side of the plano-convex lens.

Optionally, the illumination and fluorescence excitation assembly further comprises a mounting frame for at least partially being above the cell sample;

the white light lamp beads and the two fluorescence excitation lamp groups are respectively arranged on one side end face of the mounting frame, which faces the cell sample.

The present utility model also provides a cell counter comprising:

the cell sample cell comprises a light-transmitting substrate, wherein a chip placement area is formed on the upper end surface of the light-transmitting substrate and used for placing a cell sample;

the illumination and fluorescence excitation assembly is arranged on the light-transmitting substrate and is positioned above the chip placement area and used for illuminating the cell sample; the method comprises the steps of,

the image acquisition component is arranged below the light-transmitting substrate and corresponds to the chip placement area and is used for acquiring images of cells irradiated by the illumination and fluorescence excitation component;

wherein the illumination and fluorescence excitation assembly is configured as described above.

Optionally, the mounting frame of the illumination and fluorescence excitation assembly comprises a support rod and a mounting rod formed by bending and extending outwards from the upper end part of the support rod, and the lower end part of the support rod is arranged on the upper end surface of the light-transmitting substrate, so that the mounting rod is positioned above the chip placement area;

the white light lamp beads and the two fluorescent excitation lamp groups of the illumination and fluorescent excitation assembly are respectively arranged on one side end face of the mounting rod, which faces the chip placement area.

In the technical scheme of the utility model, the illumination and fluorescence excitation assembly comprises a white light lamp bead and two fluorescence excitation lamp groups; the light emitting end of the white light lamp bead is arranged towards the cell sample so that the white light emitted by the white light lamp bead irradiates the cell sample, and at the moment, the image acquisition component of the cell counter can acquire a white light bright field picture of the cell sample; the two fluorescent excitation lamp components are arranged on two sides of the white light lamp bead, the light emitting ends of the two fluorescent excitation lamp groups are respectively arranged towards the cell sample, so that excitation light emitted by the two fluorescent excitation lamp groups can respectively irradiate the cell sample and respectively excite the cell sample to emit corresponding fluorescence, and at the moment, the image acquisition component of the cell counter can acquire fluorescent pictures of the cell sample; thus, the image acquisition of the cells can be completed; meanwhile, the illumination and fluorescence excitation assembly is only composed of the white light lamp beads and the two fluorescence excitation lamp groups, so that the structure is simple, the occupied space of the illumination and fluorescence excitation assembly is reduced, and the volume of the cell counter is further reduced.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and other drawings may be obtained according to the structures shown in these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of an embodiment of an illumination and fluorescence excitation assembly according to the present utility model;

FIG. 2 is a schematic illustration of the illumination and fluorescence excitation assembly of FIG. 1 illuminating a cell sample;

FIG. 3 is a schematic diagram illustrating an internal structure of the first fluorescent lamp set of FIG. 1;

fig. 4 is a schematic structural diagram of the illumination and fluorescence excitation assembly of fig. 1 mated with an image acquisition assembly.

Description of the embodiments of the utility model the reference numerals:

reference numerals	Name of the name	Reference numerals	Name of the name
				100	Illumination and fluorescence excitation assembly	24	Light-transmitting shell
1	White light lamp bead	3	Second fluorescent excitation lamp set
				2	First fluorescent excitation lamp group	4	Mounting rack
21	First excitation light bead	41	Support bar
				22	First optical filter	42	Mounting rod
23	Collimating lens	200	Cell sample
				231	Plano-convex lens	300	Image acquisition assembly

The achievement of the objects, functional features and advantages of the present utility model will be further described with reference to the accompanying drawings, in conjunction with the embodiments.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that, if directional indications (such as up, down, left, right, front, and rear … …) are included in the embodiments of the present utility model, the directional indications are merely used to explain the relative positional relationship, movement conditions, etc. between the components in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indications are correspondingly changed.

In addition, if there is a description of "first", "second", etc. in the embodiments of the present utility model, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and/or" as it appears throughout includes three parallel schemes, for example "A and/or B", including the A scheme, or the B scheme, or the scheme where A and B are satisfied simultaneously. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present utility model.

In cell biology research, observation and calculation of the number of cell morphology are required. The existing cell image cell identification and counting method is based on a single-view image shot under a microscope, and the existing cell counter is large in size due to the internal structure, so that the cell image cell identification and counting method is inconvenient to carry and move and occupies more space; meanwhile, in the existing cell counter, the structure of a mechanism for collecting the cell image is complex, so that the occupied space of the structure for fluorescence excitation is large, and the design of the cell counter for reducing the volume is difficult to realize.

In view of this, the present utility model provides an illumination and fluorescence excitation assembly and a cytometer. Fig. 1 to 4 illustrate an embodiment of an illumination and fluorescence excitation assembly according to the present utility model.

Referring to fig. 1 and 2, the illumination and fluorescence excitation assembly 100 is used in a cytometer, and the illumination and fluorescence excitation assembly 100 includes a white light bulb 1 and two fluorescence excitation light groups; the white light lamp bead 1 is arranged above the cell sample 200, and the light emitting end of the white light lamp bead 1 is arranged towards the cell sample 200, so that the white light emitted from the white light lamp bead 1 irradiates the cell sample 200; the two fluorescent excitation lamp groups are arranged above the cell sample 200, the two fluorescent excitation lamp groups are arranged on two sides of the white light lamp bead 1 and are arranged oppositely, and the light emitting ends of the two fluorescent excitation lamp groups are arranged towards the cell sample 200 respectively, so that excitation light emitted by the two fluorescent excitation lamp groups can irradiate the cell sample 200 respectively and are used for exciting the cell sample 200 to emit corresponding fluorescence respectively.

In the technical scheme of the utility model, the illumination and fluorescence excitation assembly 100 comprises a white light lamp bead 1 and two fluorescence excitation lamp groups; the light emitting end of the white light lamp bead 1 is arranged towards the cell sample 200, so that the white light emitted from the white light lamp bead 1 irradiates the cell sample 200, and at this time, the image acquisition component 300 of the cell counter can acquire a white light bright field picture of the cell sample 200; the two fluorescent excitation lamp components are arranged at two sides of the white light lamp bead 1, and the light emitting ends of the two fluorescent excitation lamp groups are respectively arranged towards the cell sample 200, so that excitation light emitted by the two fluorescent excitation lamp groups can respectively irradiate the cell sample 200 and respectively excite the cell sample 200 to emit corresponding fluorescence, and at the moment, the image acquisition component 300 of the cell counter can acquire fluorescent pictures of the cell sample 200; thus, the image acquisition of the cells can be completed; meanwhile, since the illumination and fluorescence excitation assembly 100 is only composed of the white light lamp bead 1 and the two fluorescence excitation lamp groups, the structure is simple, thereby reducing the occupied space of the illumination and fluorescence excitation assembly 100 and further reducing the volume of the cell counter.

It should be noted that, the specific forms of the two fluorescent excitation lamp sets are not limited in the utility model, and the two fluorescent excitation lamp sets may be the same or different; in this embodiment, the two fluorescent excitation lamp sets are different in type, so that the excitation light emitted from the two fluorescent excitation lamp sets can respectively irradiate the cell sample 200, so as to respectively excite the cell sample 200 to emit different fluorescent light, thereby achieving different detection purposes.

Specifically, the two fluorescent excitation light groups include a first fluorescent excitation light group 2 and a second fluorescent excitation light group 3, the first fluorescent excitation light group 2 being formed with a first excitation light irradiation region at a position corresponding to the cell sample 200, and the second fluorescent excitation light group 3 being formed with a second excitation light irradiation region at a position corresponding to the cell sample 200; wherein the first excitation light irradiation area and/or the second excitation light irradiation area are/is overlapped with a white light irradiation area formed by the white light lamp beads; that is, the first excitation light irradiation region may overlap with the white light irradiation region formed by the white light beads, the second excitation light irradiation region may overlap with the white light irradiation region formed by the white light beads, or the first excitation light irradiation region, the second excitation light irradiation region, and the white light irradiation region formed by the white light beads may overlap with each other; in this way, it is possible to irradiate the cell sample 200 when any one of the first fluorescent lamp group 2, the second fluorescent lamp group 3 and the white light beads 1 is turned on.

Meanwhile, the two fluorescent excitation lamp groups are symmetrically arranged on two sides of the white light lamp bead 1, an included angle is formed between the central axis of each fluorescent excitation lamp group and the central axis of the white light lamp bead 1, and the included angle is alpha, wherein alpha is more than 0 degrees and less than 90 degrees; by the arrangement, the positions of the first fluorescent excitation lamp group 2, the second fluorescent excitation lamp group 3 and the white light lamp beads 1 can be distributed more reasonably, and the structural arrangement of the first fluorescent excitation lamp group 2, the second fluorescent excitation lamp group 3 and the white light lamp beads 1 is more compact.

The specific numerical value of the included angle is not limited in the present utility model, specifically, in this embodiment, an included angle α=45° between the central axis of each fluorescent-excitation lamp group and the central axis of the white-light lamp bead 1; by the arrangement, the positions of the first fluorescent excitation lamp group 2, the second fluorescent excitation lamp group 3 and the white light lamp beads 1 can be distributed more reasonably, and the structural arrangement of the first fluorescent excitation lamp group 2, the second fluorescent excitation lamp group 3 and the white light lamp beads 1 is more compact.

Referring to fig. 3, each fluorescent lamp group includes an excitation light bead 21, a light filter 22 and a collimating lens 23 sequentially arranged at intervals side by side, wherein a light emitting side of the collimating lens 23 is arranged towards the cell sample 200, so that excitation light emitted from a light emitting end of the excitation light bead 21 sequentially passes through the light filter 22 for filtering and the collimating lens 23 for collimation and then is emitted to the cell sample 200; wherein the light emitting end of the fluorescent excitation lamp group comprises the light emitting end of the excitation light beads 21; that is, after the light emitted from the light emitting end of the excitation light bead 21 is filtered by the optical filter 22 and collimated by the collimating lens 23, the collimated light is emitted to the cell sample 200, so that the coverage area of the excitation light irradiation area formed on the cell sample 200 by the fluorescent excitation lamp set can be increased, so that the cell sample 200 can be completely irradiated by the excitation light emitted from the light emitting end of the excitation light bead 21, and the excitation effect of the fluorescent excitation lamp set is improved.

Specifically, each fluorescent excitation lamp group further includes a light-transmitting housing 24, and a mounting cavity is formed in the light-transmitting housing 24; wherein the excitation light beads 21, the optical filters 22 and the collimating lens 23 are arranged in the mounting cavity; by arranging the light-transmitting shell 24, on the premise of not influencing the transmission of the excitation light emitted by the excitation light beads 21, the structure arrangement of the excitation light beads 21, the optical filters 22 and the collimating lenses 23 is more compact, and the excitation light beads 21, the optical filters 22 and the collimating lenses 23 can be protected, so that the service life of the fluorescent excitation light group is prolonged.

More specifically, the collimator lens 23 is provided as a plano-convex lens 231, and the convex side of the plano-convex lens 231 is configured to be disposed toward the cell sample 200; wherein the light-emitting side of the collimator lens 23 includes the convex side of the plano-convex lens 231; by arranging the plano-convex lens 231, the excitation light emitted from the light emitting end of the excitation light lamp bead 21 is collimated and homogenized by the plano-convex lens 231 to form parallel light to be emitted to the cell sample 200, so that the excitation effect of the fluorescent excitation lamp set is improved.

The convex diameter of the plano-convex lens 231 is not limited in the present utility model, and in this embodiment, the convex diameter of the plano-convex lens 231 is set to 6mm.

In the present utility model, the illumination and fluorescence excitation assembly 100 further comprises a mounting frame 4, the mounting frame 4 being at least partially adapted to be positioned above the cell sample 200; the white light lamp beads 1 and the two fluorescence excitation lamp groups are respectively arranged on the end face of one side of the mounting frame 4, which faces the cell sample 200; by the arrangement, the white light lamp bead 1 and the two fluorescent excitation lamp groups are more stably installed.

The present utility model also provides a cell counter, referring to fig. 4, which includes a light-transmitting substrate, an illumination and fluorescence excitation assembly 100, and an image acquisition assembly 300; a chip placement area is formed on the upper end surface of the light-transmitting substrate and used for placing the cell sample 200; the illumination and fluorescence excitation assembly 100 is disposed on the light-transmitting substrate and above the chip placement area, and is used for illuminating the cell sample 200; the image acquisition component 300 is arranged below the light-transmitting substrate, and the image acquisition component 300 is arranged corresponding to the chip placement area and is used for acquiring images of cells irradiated by the illumination and fluorescence excitation component 100; by the arrangement, the structural arrangement of the cell counter is more compact, so that the occupied space of the cell counter is reduced, and the volume of the cell counter is further reduced.

It should be noted that, the illumination and fluorescence excitation assembly 100 is configured as the above-mentioned illumination and fluorescence excitation assembly 100, that is, the cytometer includes all the technical features of all the embodiments of the above-mentioned illumination and fluorescence excitation assembly 100, and the cytometer also has all the advantages brought by the technical features, which are not described in detail herein.

That is, the white light emitted from the light emitting end of the white light bulb 1 irradiates the cell sample 200, and at this time, the image acquisition component 300 may acquire a white light bright field picture of the cell sample 200 through the light-transmitting substrate; the two fluorescent excitation lamp components are arranged at two sides of the white light lamp bead 1, and light emitting ends of the two fluorescent excitation lamp groups are respectively arranged towards the cell sample 200, so that excitation light emitted by the two fluorescent excitation lamp groups can respectively irradiate the cell sample 200 and respectively excite the cell sample 200 to emit corresponding fluorescence, and at the moment, the image acquisition assembly 300 can acquire fluorescent pictures of the cell sample 200 through the light-transmitting substrate; thus, image acquisition of the cells can be completed.

Specifically, the mounting frame 4 of the lighting and fluorescence excitation assembly 100 includes a support rod 41 and a mounting rod 42 formed by bending and extending outwards from an upper end portion of the support rod 41, and a lower end portion of the support rod 41 is disposed on an upper end surface of the light-transmitting substrate, so that the mounting rod 42 is located above the chip placement area; wherein, the white light lamp beads 1 and the two fluorescent excitation lamp groups of the illumination and fluorescent excitation assembly 100 are respectively arranged on the end face of one side of the mounting rod 42 facing the chip placement area; by the arrangement, the white light lamp bead 1 and the two fluorescent excitation lamp groups are more stably installed; and the mounting positions of the white light lamp bead 1 and the two fluorescent excitation lamp groups are more reasonable.

The foregoing description is only of the preferred embodiments of the present utility model and is not intended to limit the scope of the utility model, and all equivalent structural changes made by the specification and drawings of the present utility model or direct/indirect application in other related technical fields are included in the scope of the present utility model.

Claims (8)

1. An illumination and fluorescence excitation assembly for use in a cytometer, the illumination and fluorescence excitation assembly comprising:

the white light lamp bead is arranged above the cell sample, and the light emitting end of the white light lamp bead is arranged towards the cell sample so that white light emitted by the white light lamp bead irradiates the cell sample; the method comprises the steps of,

the two fluorescent excitation lamp groups are arranged above the cell sample, the two fluorescent excitation lamp components are arranged on two sides of the white light lamp beads and are oppositely arranged, and the light emitting ends of the two fluorescent excitation lamp groups are respectively arranged towards the cell sample, so that excitation light emitted by the two fluorescent excitation lamp groups can respectively irradiate the cell sample and are used for respectively exciting the cell sample to emit corresponding fluorescence;

each fluorescent excitation lamp group comprises excitation lamp beads, an optical filter and a collimating lens which are sequentially arranged side by side at intervals, wherein the light-emitting side of the collimating lens is arranged towards a cell sample, so that excitation light emitted from the light-emitting end of the excitation lamp beads sequentially passes through the optical filter for filtering and the collimating lens for collimation and then is emitted to the cell sample;

wherein the light-emitting end of the fluorescence excitation lamp group comprises the light-emitting end of the excitation lamp bead;

each fluorescent excitation lamp group further comprises a light-transmitting shell, and an installation cavity is formed in the light-transmitting shell;

the excitation light beads, the optical filters and the collimating lenses are arranged in the mounting cavity.

2. The illumination and fluorescence excitation assembly of claim 1, wherein the two fluorescence excitation light sets comprise a first fluorescence excitation light set and a second fluorescence excitation light set, the first fluorescence excitation light set forming a first excitation light illumination region at a location corresponding to the cell sample and the second fluorescence excitation light set forming a second excitation light illumination region at a location corresponding to the cell sample;

the first excitation light irradiation area and/or the second excitation light irradiation area are/is overlapped with the white light irradiation area formed by the white light lamp beads.

3. A lighting and fluorescent light excitation assembly as recited in claim 1, wherein two of said fluorescent light excitation lamp groups are symmetrically disposed on opposite sides of said white light bulb, each of said fluorescent light excitation lamp groups having a central axis disposed at an angle α with respect to a central axis of said white light bulb, wherein 0 ° < α < 90 °.

4. A lighting and fluorescent light-excitation assembly as claimed in claim 3, wherein the angle α = 45 ° between the central axis of each of the fluorescent light-excitation lamp groups and the central axis of the white light beads.

5. The illumination and fluorescence excitation assembly of claim 1, wherein the collimating lens is configured as a plano-convex lens with a convex side of the plano-convex lens configured to be disposed toward the cell sample;

wherein the light-emitting side of the collimating lens comprises the convex side of the plano-convex lens.

6. The illumination and fluorescence excitation assembly of claim 1, further comprising a mount for at least partially above a cell sample;

the white light lamp beads and the two fluorescence excitation lamp groups are respectively arranged on one side end face of the mounting frame, which faces the cell sample.

7. A cytometer, comprising:

the cell sample cell comprises a light-transmitting substrate, wherein a chip placement area is formed on the upper end surface of the light-transmitting substrate and used for placing a cell sample;

the illumination and fluorescence excitation assembly of any one of claims 1 to 6, disposed above the light transmissive substrate and above the chip placement area for illuminating a cell sample; the method comprises the steps of,

the image acquisition component is arranged below the light-transmitting substrate and corresponds to the chip placement area and is used for acquiring images of cells irradiated by the illumination and fluorescence excitation component.

8. The cytometer of claim 7, wherein the mounting frame of the illumination and fluorescence excitation assembly comprises a support bar and a mounting bar formed by bending and extending outwards from the upper end of the support bar, and the lower end of the support bar is disposed on the upper end surface of the light-transmitting substrate, so that the mounting bar is above the chip placement area;

the white light lamp beads and the two fluorescent excitation lamp groups of the illumination and fluorescent excitation assembly are respectively arranged on one side end face of the mounting rod, which faces the chip placement area.