CN220183281U - Immobilized enzyme catalytic reaction device - Google Patents
Immobilized enzyme catalytic reaction device Download PDFInfo
- Publication number
- CN220183281U CN220183281U CN202321463441.6U CN202321463441U CN220183281U CN 220183281 U CN220183281 U CN 220183281U CN 202321463441 U CN202321463441 U CN 202321463441U CN 220183281 U CN220183281 U CN 220183281U
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- China
- Prior art keywords
- immobilized enzyme
- reaction
- cover
- catalytic reaction
- reaction device
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- 108010093096 Immobilized Enzymes Proteins 0.000 title claims abstract description 36
- 238000006555 catalytic reaction Methods 0.000 title claims abstract description 17
- 238000006243 chemical reaction Methods 0.000 claims abstract description 43
- 238000007789 sealing Methods 0.000 claims abstract description 9
- 230000008878 coupling Effects 0.000 claims description 6
- 238000010168 coupling process Methods 0.000 claims description 6
- 238000005859 coupling reaction Methods 0.000 claims description 6
- 238000009434 installation Methods 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000012423 maintenance Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 239000012074 organic phase Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 230000006872 improvement Effects 0.000 description 3
- 239000008346 aqueous phase Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to an immobilized enzyme catalytic reaction device, which belongs to the technical field of bioreactors and comprises a reaction tank and a tank cover, wherein a reaction component is arranged in the reaction tank and comprises a driving motor, the driving motor is arranged at the top end of the tank cover, the output end of the driving motor penetrates through the tank cover and is connected with a rotating block, an installation groove is formed in the inner side wall of the reaction tank, an installation plate is arranged at the top end of the installation groove, an inserting groove is formed in the top end of the installation plate, the inserting groove is matched with the rotating block, a plurality of through hole columns are connected at the bottom end of the installation plate in an equal division mode, a sealing plate is connected at the top end of the installation plate, and a plurality of bulges matched with the through hole columns are connected at the bottom end of the sealing plate in an equal division mode. The utility model has the advantages of reducing the damage to the immobilized enzyme, prolonging the service life of the immobilized enzyme, reducing the maintenance frequency of equipment, saving the production cost, providing good reaction conditions and ensuring the normal operation of the reaction.
Description
Technical Field
The utility model relates to the technical field of bioreactors, in particular to an immobilized enzyme catalytic reaction device.
Background
The immobilized enzyme technology is a technology which uses physical or chemical means to seal and lock solid materials or limit the free enzyme in a certain area to perform active and special catalysis, and can be recycled for a long time.
The utility model of Chinese patent application No. CN202221908578.3 discloses a two-phase catalytic reaction device suitable for immobilized enzyme, which comprises a reaction tank and a stirring device arranged at the top of the reaction tank, wherein the reaction tank is sequentially provided with an organic phase liquid inlet, an organic phase liquid outlet, an aqueous phase liquid inlet and an aqueous phase liquid outlet from top to bottom; the method is characterized in that: still including holding the box with a net of immobilized enzyme, box with a net is connected with the retort inner wall, and is located the organic phase region in the retort, the box with a net top is equipped with the dog-house, agitating unit's stirring end passes the dog-house and stretches into inside the box with a net, immobilized enzyme is located the box with a net, the mesh diameter of box with a net is less than the particle diameter of immobilized enzyme. The immobilized enzyme particles are convenient to recycle, the immobilized enzyme particles can be recycled from the organic phase only by taking out the net cage, and the reaction of multiple batches can be easily realized after substrate replacement.
The above comparative document has the following problems:
the device is arranged through the detachable net cage and the stirring device, and the immobilized enzyme particles can be conveniently recovered and replaced on the premise of completing catalytic reaction, but the stirring blades are in direct contact with the immobilized enzyme particles during rotation, and the shearing acting force during rotation can possibly damage the structure of the immobilized enzyme particles, so that the service life of the immobilized enzyme is influenced.
Disclosure of Invention
The present utility model is directed to an immobilized enzyme catalytic reaction device, which solves the problems set forth in the background art.
The technical scheme of the utility model is as follows: the utility model provides an immobilized enzyme catalytic reaction device, includes retort and cover, the inside of retort is provided with reaction unit, reaction unit includes driving motor, driving motor installs in the top of cover, driving motor's output runs through the cover to be connected with the rotating block, the mounting groove has been seted up to the inside wall of retort, the top of mounting groove is provided with the mounting panel, the slot has been seted up on the top of mounting panel, slot and rotating block assorted, the bottom of mounting panel is equally divided to be connected with a plurality of through-hole posts, the top of mounting panel is connected with the shrouding, the bottom of shrouding equally divided be connected with a plurality of with through-hole post assorted arch.
Furthermore, a plurality of spoilers are equally connected to the inner side wall of the reaction tank.
Further, the bottom of cover is connected with the sealing washer, the bottom of mounting panel is connected with the ball.
Further, the bottom of cover is provided with coupling assembling, coupling assembling includes three fastener and plug-in components, and is three the fastener is equallyd divide and is connected in the outside of retort, and is three the plug-in components equallyd divide and are connected in the bottom of cover, the fastener is the L font, the plug-in components is the U font, the fastener cooperatees with the plug-in components, the outside equallyd divide of retort is connected with three hasp.
Further, the spacing size between the clamping piece and the hasp is larger than the length size of the clamping piece.
Further, one end of the reaction tank is connected with an observation window, and one end of the reaction tank is provided with a temperature sensor and a PH value sensor.
The utility model provides an immobilized enzyme catalytic reaction device through improvement, which has the following improvement and advantages compared with the prior art:
the method comprises the following steps: according to the utility model, through the arrangement of the mounting plate and the through hole column and the torque transmission function of the rotating block and the slot, the mounting plate drives the through hole column to rotate, the immobilized enzyme is prevented from being damaged by shearing force while stirring materials, the damage to the immobilized enzyme is reduced, the service life of the immobilized enzyme is prolonged, the maintenance frequency of equipment is reduced, and the production cost is saved.
And two,: according to the utility model, through the arrangement of the clamping piece and the plug-in piece and the locking effect of the hasp, the sealing strength of the reaction tank and the tank cover is enhanced, and the arrangement of the observation window, the temperature sensor and the PH value sensor is utilized to intuitively feed back the reaction condition of the reactant, so that the user can conveniently and timely adjust the reaction condition, provide good reaction condition for the reaction and ensure the normal reaction.
Drawings
The utility model is further explained below with reference to the drawings and examples:
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a schematic cross-sectional elevation view of the present utility model;
FIG. 3 is an enlarged schematic view of area A in FIG. 2;
fig. 4 is a schematic top view of the present utility model.
Reference numerals illustrate: 1. a reaction tank; 2. a connection assembly; 21. a clamping piece; 22. an insert; 23. a hasp; 3. a can lid; 4. a reaction assembly; 41. a driving motor; 42. a rotating block; 43. a sealing plate; 44. a mounting plate; 45. a via post; 46. a spoiler; 47. a seal ring; 48. a mounting groove; 49. a slot; 5. an observation window; 6. a temperature sensor; 7. a PH sensor.
Detailed Description
The following detailed description of the present utility model will provide clear and complete description of the technical solutions of the embodiments of the present utility model, with reference to fig. 1 to 4, and it is obvious that the described embodiments are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The utility model provides an immobilized enzyme catalytic reaction device through improvement, as shown in figures 1-4, comprising a reaction tank 1 and a tank cover 3, wherein a reaction assembly 4 is arranged in the reaction tank 1, the reaction assembly 4 comprises a driving motor 41, the driving motor 41 is arranged at the top end of the tank cover 3, the output end of the driving motor 41 penetrates through the tank cover 3 and is connected with a rotating block 42, the inner side wall of the reaction tank 1 is provided with a mounting groove 48, the top end of the mounting groove 48 is provided with a mounting plate 44, the top end of the mounting plate 44 is provided with a slot 49, the slot 49 is matched with the rotating block 42, the bottom end of the tank cover 3 is connected with a sealing ring 47, the bottom end of the mounting plate 44 is connected with a ball, thereby improving the sealing performance of the device by utilizing the pressing action of the sealing ring 47 and avoiding gas leakage, and facilitating the rotation of the mounting plate 44 by utilizing the arrangement of the ball, reduce rotation resistance, the bottom of mounting panel 44 is divided equally and is connected with a plurality of through-hole posts 45, and the through-hole posts 45 is hollow setting, and inside is provided with immobilized enzyme, and the outside of through-hole posts 45 is provided with a plurality of diameters and is less than immobilized enzyme granule diameter's through-hole, the top of mounting panel 44 is connected with shrouding 43, shrouding 43's bottom divided equally and is connected with a plurality of and through-hole post 45 assorted arch, retort 1's inside wall divided equally is connected with a plurality of spoilers 46 to utilize spoiler 46 to carry out the vortex to the material, reduce the production of whirl phenomenon, ensure that the material fully reacts with immobilized enzyme, the bottom of cover 3 is provided with coupling assembling 2, coupling assembling 2 includes three fastener 21 and plug-in components 22, and three fastener 21 divided equally are connected in retort 1's outside, three plug-in components 22 divided equally are connected in the bottom of cover 3, the clamping piece 21 is L-shaped, the plug-in component 22 is U-shaped, the clamping piece 21 cooperates with the plug-in component 22, three buckles 23 are equally divided and connected to the outside of the reaction tank 1, so that the reaction tank 1 is tightly combined with the tank cover 3 by utilizing the cooperation of the clamping piece 21 and the plug-in component 22, the space between the clamping piece 21 and the buckles 23 is larger than the length of the clamping piece 21, the plug-in component 22 cannot interfere with the buckles 23 during installation, one end of the reaction tank 1 is connected with an observation window 5, and the temperature sensor 6 and the PH value sensor 7 are arranged at one end of the reaction tank 1, so that direct feedback on the internal condition of the reaction tank 1 is realized by utilizing the arrangement of the observation window 5, the temperature sensor 6 and the PH value sensor 7, and a user can conveniently perform corresponding adjustment work.
Working principle: when the device is used, the water phase and organic phase materials are thrown into the tank body of the reaction tank 1 by utilizing the interface on the reaction tank 1, then the driving motor 41 is started to drive the rotating block 42 to rotate, the torque transmission effect of the rotating block 42 and the slot 49 is utilized to enable the mounting plate 44 to rotate, friction resistance during rotation is reduced by utilizing the balls, then the mounting plate 44 drives the through hole column 45 to rotate, the materials are stirred by utilizing the through hole column 45 and are matched with the spoiler 46, the materials rotate and react with immobilized enzyme in the through hole column 45, in the reaction process, the device can utilize the observation window 5, the temperature sensor 6 and the PH value sensor 7 to directly feed back the condition in the reaction tank 1, a user can correspondingly adjust according to the reaction conditions, when the immobilized enzyme needs to be replaced, the constraint of the hasp 23 is released, the tank cover 3 is rotated, the clamping piece 21 and the plug-in piece 22 are misplaced, the tank cover 3 can be removed, and the mounting plate 44 and the through hole column 45 are taken out, and the immobilized enzyme replacement operation is carried out.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present utility model. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present utility model is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. An immobilized enzyme catalytic reaction device is characterized in that: including retort (1) and cover (3), the inside of retort (1) is provided with reaction subassembly (4), reaction subassembly (4) are including driving motor (41), driving motor (41) are installed in the top of cover (3), the output of driving motor (41) runs through cover (3) to be connected with rotating block (42), mounting groove (48) have been seted up to the inside wall of retort (1), the top of mounting groove (48) is provided with mounting panel (44), slot (49) have been seted up on the top of mounting panel (44), slot (49) and rotating block (42) assorted, the bottom of mounting panel (44) is equally divided and is connected with a plurality of through-hole post (45), the top of mounting panel (44) is connected with shrouding (43), the bottom of shrouding (43) equally divided and be connected with a plurality of and through-hole post (45) assorted arch.
2. The immobilized enzyme catalytic reaction device according to claim 1, wherein: the inner side wall of the reaction tank (1) is equally connected with a plurality of spoilers (46).
3. The immobilized enzyme catalytic reaction device according to claim 1, wherein: the bottom of cover (3) is connected with sealing washer (47), the bottom of mounting panel (44) is connected with the ball.
4. The immobilized enzyme catalytic reaction device according to claim 1, wherein: the bottom of cover (3) is provided with coupling assembling (2), coupling assembling (2) include three fastener (21) and plug-in components (22), and is three fastener (21) are equallyd divide and are connected in the outside of retort (1), and is three plug-in components (22) are equallyd divide and are connected in the bottom of cover (3), fastener (21) are the L font, plug-in components (22) are the U font, fastener (21) cooperatees with plug-in components (22), the outside of retort (1) is equallyd divide and is connected with three hasp (23).
5. The immobilized enzyme catalytic reaction device according to claim 4, wherein: the spacing size of the clamping piece (21) and the hasp (23) is larger than the length size of the clamping piece (21).
6. The immobilized enzyme catalytic reaction device according to claim 1, wherein: one end of the reaction tank (1) is connected with an observation window (5), and one end of the reaction tank (1) is provided with a temperature sensor (6) and a PH value sensor (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321463441.6U CN220183281U (en) | 2023-06-08 | 2023-06-08 | Immobilized enzyme catalytic reaction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321463441.6U CN220183281U (en) | 2023-06-08 | 2023-06-08 | Immobilized enzyme catalytic reaction device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220183281U true CN220183281U (en) | 2023-12-15 |
Family
ID=89111668
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321463441.6U Active CN220183281U (en) | 2023-06-08 | 2023-06-08 | Immobilized enzyme catalytic reaction device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220183281U (en) |
-
2023
- 2023-06-08 CN CN202321463441.6U patent/CN220183281U/en active Active
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