CN220183279U

CN220183279U - Immobilized enzyme reactor with stirring device

Info

Publication number: CN220183279U
Application number: CN202321457066.4U
Authority: CN
Inventors: 姜艳军; 郑晓冰; 王立晖; 高静; 贺莹; 李赫宇
Original assignee: Hebei University of Technology
Current assignee: Hebei University of Technology
Priority date: 2023-06-08
Filing date: 2023-06-08
Publication date: 2023-12-15
Anticipated expiration: 2033-06-08

Abstract

The utility model relates to an immobilized enzyme reactor with a stirring device, which belongs to the technical field of bioreactors and comprises a reactor body, an observation window and a sealing cover, wherein a stirring assembly is arranged in the reactor body and comprises a driving motor, the driving motor is arranged at the top end of the reactor body, the output end of the driving motor penetrates through the sealing cover and is connected with a rotating shaft, the outer part of the rotating shaft is connected with a plurality of stirring blades in an equal part, the outer part of the rotating shaft is provided with a plurality of enzyme carrying plates in an equal part, one end of the enzyme carrying plates is connected with a connecting plate, one end of the rotating shaft is provided with a connecting groove, the connecting plate is connected with the inside of the connecting groove, the top end of the connecting groove is connected with a baffle through a spring, and one end of the baffle penetrates through the rotating shaft and is connected with a pushing plate. The utility model has the advantages of improving the convenience of equipment maintenance, facilitating the timely replacement of the immobilized enzyme, ensuring the full reaction of the immobilized enzyme and the materials, simultaneously conveniently adjusting the environmental temperature of the materials during processing and improving the reaction efficiency of the materials.

Description

Immobilized enzyme reactor with stirring device

Technical Field

The utility model relates to the technical field of bioreactors, in particular to an immobilized enzyme reactor with a stirring device.

Background

The immobilized enzyme technology is a technology which uses physical or chemical means to seal and lock solid materials or limit the free enzyme in a certain area to perform active and special catalysis, and can be recycled for a long time.

The utility model of Chinese patent application No. CN202222174232.1 discloses an enzyme reaction device for a viscous system, which comprises a reactor, wherein a discharging pipe is fixedly arranged at the upper end of the reactor, a feeding pipe is fixedly arranged at the bottom end of the reactor, a stirring structure is arranged in the reactor and comprises a stirring rod and a motor, the motor is fixedly arranged at the side end of the reactor, the stirring rod is rotatably arranged in the reactor, the end part of the stirring rod is fixedly connected with an output shaft of the motor, a stirring paddle is fixedly arranged on the stirring rod, the stirring paddle is in a spiral shape, a detector is fixedly arranged on the inner wall of the reactor close to the discharging pipe, the enzyme reaction device for the viscous system is more suitable for the reaction of viscous materials and enzymes, and the stirring paddle has the functions of pushing, conveying and mixing, and can push a substrate in a tank and promote the contact and the mixing of the substrate and enzymes by adjusting the stirring speed.

The above comparative document has the following problems:

above-mentioned equipment stirs and carries the material through helical stirring rake, but glues thick material and glues on the stirring rake, and then arouses the jam of device, and immobilized enzyme sets up on the stirring rake in the above-mentioned equipment, when needing to change immobilized enzyme, the dismantlement structure of device is inconvenient relatively, therefore has certain shortcoming.

Disclosure of Invention

The utility model aims to provide an immobilized enzyme reactor with a stirring device, which solves the problems in the prior art.

The technical scheme of the utility model is as follows: the utility model provides a take agitating unit's immobilized enzyme reactor, includes reactor body and observation window and closing cap, the inside of reactor body is provided with stirring subassembly, stirring subassembly includes driving motor, driving motor installs in the top of reactor body, driving motor's output runs through the closing cap to be connected with the axis of rotation, the outside of axis of rotation is equallyd divide and is connected with a plurality of stirring leaves, the outside of axis of rotation is equallyd divide and is provided with a plurality of year enzyme boards, the one end of year enzyme board is connected with the connecting plate, the spread groove has been seted up to the one end of axis of rotation, the connecting plate is connected in the inside of spread groove, the top of spread groove has the baffle through spring coupling, the one end of baffle runs through the axis of rotation to be connected with the push pedal.

Further, two scrapers are symmetrically connected to the outer part of the rotating shaft, and the length and width dimensions of the scrapers are equal to the inner diameter dimensions of the reactor body.

Further, the stirring blades and the enzyme carrying plates are arranged at intervals in the vertical direction, the stirring blades and the enzyme carrying plates are arranged outside the rotating shaft at equal intervals, the axes of the stirring blades and the axes of the rotating shaft are arranged at an included angle, the axes of the enzyme carrying plates and the axes of the rotating shaft are arranged at an included angle, and the inclination directions of the stirring blades and the enzyme carrying plates are opposite.

Further, the bottom of closing cap is provided with coupling assembling, coupling assembling includes a plurality of L shape fastener and U-shaped fastener, and a plurality of L shape fastener partition is connected in the outside of reactor body, and a plurality of U-shaped fastener partition is connected in the bottom of closing cap, the equal through connection in top of closing cap has the bolt, the bolt is connected with the closing cap through the spring, the outside partition of reactor body is connected with a plurality of plug-ins.

Further, the horizontal height of the insert is lower than that of the U-shaped clamping piece.

Further, an electric heating plate is arranged in the reactor body, and a heat preservation layer is arranged in the reactor body.

The utility model provides an immobilized enzyme reactor with a stirring device through improvement, which has the following improvement and advantages compared with the prior art:

the method comprises the following steps: according to the utility model, through the arrangement of the enzyme loading plate and the stirring blade, the stirring and turbulent flow of the materials are realized, the full reaction of the enzyme loading plate and the materials is facilitated, the arrangement of the connecting plate and the baffle is utilized, the assembly effect of the U-shaped clamping piece and the L-shaped clamping piece is matched, the enzyme loading plate is convenient to detach and replace, the convenience of equipment maintenance is improved, the immobilized enzyme is convenient to replace in time, and the full reaction of the immobilized enzyme and the materials is ensured.

And two,: according to the utility model, through the arrangement of the electric heating plate and the heat preservation layer, the internal temperature of the reactor body is conveniently adjusted, the environmental temperature of material reaction is conveniently adjusted, and the reaction efficiency of the materials is improved.

Drawings

The utility model is further explained below with reference to the drawings and examples:

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is a schematic cross-sectional elevation view of the present utility model;

fig. 3 is a schematic view of a partial side sectional structure of the present utility model.

Reference numerals illustrate: 1. a reactor body; 2. an observation window; 3. a connection assembly; 31. an insert; 32. a plug pin; 33. an L-shaped clamping piece; 34. a U-shaped clamping piece; 4. a cover; 5. a stirring assembly; 51. a driving motor; 52. a rotating shaft; 53. stirring the leaves; 54. a scraper; 55. enzyme-carrying board; 56. a connecting plate; 57. a push plate; 58. a baffle; 59. a connecting groove; 6. an electric heating plate.

Detailed Description

The technical solutions of the embodiments of the present utility model will be clearly and completely described below in detail with reference to fig. 1 to 3, and it is obvious that the described embodiments are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The utility model provides an immobilized enzyme reactor with a stirring device through improvement, as shown in figures 1-3, which comprises a reactor body 1, an observation window 2 and a sealing cover 4, wherein a stirring assembly 5 is arranged inside the reactor body 1, the stirring assembly 5 comprises a driving motor 51, the driving motor 51 is arranged at the top end of the reactor body 1, the output end of the driving motor 51 penetrates through the sealing cover 4 and is connected with a rotating shaft 52, the outer part of the rotating shaft 52 is connected with a plurality of stirring blades 53 in an equal part, the outer part of the rotating shaft 52 is provided with a plurality of enzyme carrying plates 55 in an equal part, the stirring blades 53 and the enzyme carrying plates 55 are arranged at intervals in the vertical direction, the axes of the stirring blades 53 and the enzyme carrying plates 55 are arranged at the outer part of the rotating shaft 52 at equal intervals, the axes of the stirring blades 53 and the axes of the rotating shaft 52 form an included angle, the axes of the enzyme carrying plates 55 and the axes of the rotating shaft 52 form an included angle, the stirring blade 53 and the enzyme-carrying plate 55 are inclined in opposite directions, so that the stirring blade 53 and the enzyme-carrying plate 55 are arranged in an inclined manner to stir materials, the reaction efficiency is accelerated, stirring blades 53 and enzyme-carrying plates 55 in different inclined directions are utilized to generate a slightly vertical turbulence effect on the materials, the generation of a swirling phenomenon is reduced, one end of the enzyme-carrying plate 55 is connected with a connecting plate 56, one end of the rotating shaft 52 is provided with a connecting groove 59, the connecting plate 56 is connected with the inside of the connecting groove 59, the top end of the connecting groove 59 is connected with a baffle plate 58 through a spring, one end of the baffle plate 58 penetrates through the rotating shaft 52 and is connected with a pushing plate 57, two scraping plates 54 are symmetrically connected with the outside of the rotating shaft 52, the length and width dimensions of the scraping plates 54 are equal to the inner diameter dimension of the reactor body 1, so that the arrangement of the scraping plates 54 is utilized, the rotation of cooperation axis of rotation 52 scrapes the inner wall of reactor body 1 and sweeps, reduces to glue the thick material of gluing on the equipment inner wall, makes things convenient for equipment to continue processing, the bottom of closing cap 4 is provided with coupling assembling 3, coupling assembling 3 includes a plurality of L shape fastener 33 and U-shaped fastener 34, and is a plurality of L shape fastener 33 partition connects in the outside of reactor body 1, a plurality of U-shaped fastener 34 partition connects in the bottom of closing cap 4, the top partition through connection of closing cap 4 has bolt 32, bolt 32 is connected with closing cap 4 through the spring, the outside partition of reactor body 1 is connected with a plurality of plug-ins 31, the level of plug-ins 31 is less than the level of U-shaped fastener 34 to avoid plug-ins 31 to cause the interference to the installation of U-shaped fastener 34, and utilize the cooperation of U-shaped fastener 34 and L shape fastener 33, carry out vertical orientation's connection to reactor body 1, and utilize 32 and plug-ins 31's locking effect to carry out axial direction to closing cap 4 and carry out the electric heater plate 1 to the axial direction, and the inside heater plate 1 is connected with the side of closing cap 4, thereby the heater plate 1 is inside to the reactor 1 is avoided to the setting up, and the temperature-insulating plate 1 is inside to be adjusted.

Working principle: when the device is used, materials are input from the feed inlet of the reactor body 1, the driving motor 51 is started, the driving motor 51 is driven to rotate the rotating shaft 52, the rotating shaft 52 drives the stirring blade 53 and the enzyme carrying plate 55 to rotate, the materials are stirred, a certain turbulence effect is achieved on the materials by utilizing the inclined stirring blade 53 and the enzyme carrying plate 55, the internal temperature of the reactor body 1 is adjusted to a proper reaction temperature by utilizing the electric heating plate 6 and the heat insulation layer, the materials are fully reacted with immobilized enzyme, meanwhile, the rotating shaft 52 drives the scraping plate 54 to rotate, the scraping plate 54 is utilized to scrape and sweep part of the materials with viscous properties, the sticky materials on the inner wall of the reactor body 1 are reduced, after the reaction is completed, the materials are discharged from the discharge port of the reactor body 1, when the immobilized enzyme needs to be replaced, the plug pin 32 is pulled out from the plug-in piece 31, the sealing cover 4 is rotated, the U-shaped clamping piece 34 and the L-shaped clamping piece 33 are misplaced, the sealing cover 4 is lifted upwards together with the stirring assembly 5, the pushing plate 57 is slid, the sealing plate 58 is retracted upwards, the enzyme carrying plate 55 is lifted upwards together with the connecting plate 56, the sealing plate 56 is separated from the connecting plate and the connecting plate 56, the enzyme carrying plate 55 is removed from the connecting groove, and the enzyme carrying plate 55 is replaced, and the enzyme carrying plate is replaced.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present utility model. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present utility model is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An immobilized enzyme reactor with a stirring device, which is characterized in that: including reactor body (1) and observation window (2) and closing cap (4), the inside of reactor body (1) is provided with stirring subassembly (5), stirring subassembly (5) are including driving motor (51), driving motor (51) are installed on the top of reactor body (1), the output of driving motor (51) runs through closing cap (4) to be connected with axis of rotation (52), the outside bisection of axis of rotation (52) is connected with a plurality of stirring leaf (53), the outside bisection of axis of rotation (52) is provided with a plurality of year enzyme board (55), the one end of year enzyme board (55) is connected with connecting plate (56), spread groove (59) have been seted up to the one end of axis of rotation (52), connecting plate (56) are connected in the inside of spread groove (59), the top of spread groove (59) is connected with baffle (58) through the spring coupling, the one end of baffle (58) runs through axis of rotation (52) and is connected with push pedal (57).

2. An immobilized enzyme reactor with stirring device according to claim 1, characterized in that: two scrapers (54) are symmetrically connected to the outer part of the rotating shaft (52), and the length and width dimensions of the scrapers (54) are equal to the inner diameter dimensions of the reactor body (1).

3. An immobilized enzyme reactor with stirring device according to claim 1, characterized in that: stirring leaf (53) are the interval setting in vertical direction with year enzyme board (55), stirring leaf (53) are equidistant to set up in the outside of axis of rotation (52) with year enzyme board (55), the axle center of stirring leaf (53) is the contained angle setting with the axle center of axis of rotation (52), the axle center of year enzyme board (55) is the contained angle setting with the axle center of axis of rotation (52), the inclination opposite of stirring leaf (53) and year enzyme board (55).

4. An immobilized enzyme reactor with stirring device according to claim 1, characterized in that: the bottom of closing cap (4) is provided with coupling assembling (3), coupling assembling (3) are including a plurality of L shape fastener (33) and U-shaped fastener (34), and is a plurality of L shape fastener (33) are divided equally and are connected in the outside of reactor body (1), a plurality of U-shaped fastener (34) are divided equally and are connected in the bottom of closing cap (4), the equal through connection in top of closing cap (4) has bolt (32), bolt (32) are connected with closing cap (4) through the spring, the outside of reactor body (1) is divided equally and is connected with a plurality of plug-ins (31).

5. The immobilized enzyme reactor with stirring device according to claim 4, wherein: the horizontal height of the insert (31) is lower than the horizontal height of the U-shaped clamping piece (34).

6. An immobilized enzyme reactor with stirring device according to claim 1, characterized in that: the reactor is characterized in that an electric heating plate (6) is arranged in the reactor body (1), and a heat preservation layer is arranged in the reactor body (1).