CN220160729U - Macroporous resin cleaning equipment for separating pichia pastoris fermentation products - Google Patents
Macroporous resin cleaning equipment for separating pichia pastoris fermentation products Download PDFInfo
- Publication number
- CN220160729U CN220160729U CN202321696520.1U CN202321696520U CN220160729U CN 220160729 U CN220160729 U CN 220160729U CN 202321696520 U CN202321696520 U CN 202321696520U CN 220160729 U CN220160729 U CN 220160729U
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- China
- Prior art keywords
- tank
- macroporous resin
- fixedly connected
- separating
- pichia pastoris
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- 239000011347 resin Substances 0.000 title claims abstract description 58
- 229920005989 resin Polymers 0.000 title claims abstract description 58
- 238000004140 cleaning Methods 0.000 title claims abstract description 51
- 238000000855 fermentation Methods 0.000 title claims abstract description 35
- 230000004151 fermentation Effects 0.000 title claims abstract description 35
- 241000235058 Komagataella pastoris Species 0.000 title claims abstract description 26
- 238000003756 stirring Methods 0.000 claims abstract description 38
- 239000000463 material Substances 0.000 claims abstract description 31
- 230000000149 penetrating effect Effects 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 49
- 239000007788 liquid Substances 0.000 claims description 30
- 239000002699 waste material Substances 0.000 claims description 12
- 238000007599 discharging Methods 0.000 claims description 9
- 239000007921 spray Substances 0.000 claims description 7
- 238000005406 washing Methods 0.000 abstract description 8
- 238000000746 purification Methods 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 18
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 244000285963 Kluyveromyces fragilis Species 0.000 description 1
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 210000003783 haploid cell Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
Landscapes
- Processing Of Solid Wastes (AREA)
Abstract
The utility model relates to the technical field of macroporous resin cleaning, and discloses macroporous resin cleaning equipment for separating fermentation products of pichia pastoris, which comprises a box body, wherein a feed inlet is formed in one side of the box body in a penetrating manner, a motor is fixedly arranged on one side of the upper end face of the box body, the output end of the motor is fixedly connected with a rotating rod, one end of the rotating rod is provided with the box body and a stirring box in a penetrating manner and is fixedly connected with a plurality of stirring paddles, a suction pipe is arranged in one side of the outer side wall of the stirring box in a penetrating manner, and a material pump is fixedly arranged at one end of the suction pipe, which is far away from the stirring box. In the present utility model, the macroporous resin washing apparatus for separating the fermentation product of pichia pastoris has a function of automatically washing macroporous resin, and it also has a function of washing the residual fermentation product in the macroporous resin by centrifugal force with stirring so as to be cleaner, and it also has a function of performing multiple washing purification.
Description
Technical Field
The utility model relates to the technical field of macroporous resin cleaning, in particular to macroporous resin cleaning equipment for separating fermentation products of pichia pastoris.
Background
Pichia pastoris is a type of yeast in methanol nutritional yeasts that can utilize methanol as the sole carbon source and energy source. Like other yeasts, it exists mainly in haploid form in asexual growth phase, and when environmental nutrition is limited, 2 conjugated haploid cells of different physiological types are often induced to mate, fuse into a double body, and when pichia pastoris is fermented, a large amount of fermentation products are produced, so that cleaning with macroporous resin is often required.
Most of the existing macroporous resin is often inconvenient to clean, manual operation is often used, the macroporous resin is taken out and cleaned first and then refilled, the cleaning efficiency is slow, and the cleaning is not clean. Accordingly, one skilled in the art would be able to provide a macroporous resin washing apparatus for separating the fermentation product of pichia pastoris that solves the problems set forth in the background art above.
Disclosure of Invention
The utility model aims to solve the defects in the prior art, and provides a macroporous resin cleaning device for separating a fermentation product of pichia pastoris, which has the function of automatically cleaning macroporous resin, and also has the function of cleaning residual fermentation products in the macroporous resin by stirring and centrifugal force so as to be cleaner, and also has the function of performing multiple cleaning and purification so that the cleaned macroporous resin is purer and more convenient to use.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
the macroporous resin cleaning equipment for separating the pichia pastoris fermentation products comprises a box body, wherein a feed inlet is formed in one side of the box body in a penetrating manner, a motor is fixedly arranged on one side of the upper end face of the box body, the output end of the motor is fixedly connected with a rotating rod, and one end of the rotating rod is provided with the box body and a stirring box in a penetrating manner and is fixedly connected with a plurality of stirring paddles;
the stirring tank is characterized in that a suction pipe is arranged on one side of the outer side wall of the stirring tank in a penetrating mode, a material pump is fixedly arranged at one end, far away from the stirring tank, of the suction pipe, a discharging pipe is fixedly connected to the output end of the material pump, a standing tank is arranged at one end, far away from the material pump, of the discharging pipe in a penetrating mode, a first liquid inlet is fixedly connected to the upper end face of the standing tank, and a discharge port is fixedly connected to one side of the standing tank.
Through above-mentioned technical scheme through setting up feed inlet, a motor, the dwang, stirring rake and agitator tank, make the device pass through the feed inlet with macroporous resin pack into the agitator tank and drive the dwang through the motor, make the stirring rake rotate, drive the inside ethanol of agitator tank, the inside fermentation of macroporous resin is washd to the centrifugal force that produces, make macroporous resin wash more cleanly, through setting up the material absorbing pipe, the material pump, discharging pipe and the case of standing, make the macroporous resin of in-device through stirring, get into the case of standing through the material pump transmission and get into in the ethanol liquid, keep stand, clear away remaining fermentation material, through setting up the wash tank, the aqueduct, a water pump, the delivery port, a backup pad, the shunt, the shower nozzle, wash the case, the filter tank, the collecting tank, second feed inlet, discharge gate and waste liquid export, pass through the wash water pipe and water pump transmission to the shunt pipe through the shower nozzle, wash the material that gets into the filter tank, make the device get automatic cleaning macroporous resin.
Further, a cleaning water tank is fixedly arranged on one side, far away from the motor, of the upper end face of the tank body, a water guide pipe is fixedly connected to the lower end face of the cleaning water tank, a water pump is fixedly arranged at one end, far away from the cleaning water tank, of the water guide pipe, a water outlet is fixedly connected to the output end of the water pump, a water diversion pipe is fixedly connected to one end of the water outlet, and a plurality of spray heads are fixedly connected to the outer side wall of the water diversion pipe;
through above-mentioned technical scheme for the device can wash purified macroporous resin automatically, makes the device use more conveniently, has saved the manpower.
Further, a cleaning box is fixedly arranged on one side, far away from the stirring box, of the inner side wall of the box body, a liquid collecting tank is fixedly arranged on the inner bottom end face of the cleaning box, a supporting plate is fixedly connected to the inner side wall of the cleaning box, and the water pump is positioned on the supporting plate;
through above-mentioned technical scheme, through setting up the collecting tank for the waste liquid of washing can be discharged.
Further, a filter tank is fixedly arranged on one side of the inner side wall of the cleaning box, and the filter tank is positioned below the spray heads;
through above-mentioned technical scheme, through setting up the shower nozzle for the device can wash macroporous resin through shower nozzle blowout rivers.
Further, one side of the liquid collecting groove is fixedly connected with a waste liquid outlet, and the waste liquid outlet is provided with a box body in a penetrating manner;
through above-mentioned technical scheme, through setting up the waste liquid export for the waste liquid of washing can be discharged.
Further, one side of the filter tank is fixedly connected with a discharge port;
through above-mentioned technical scheme, through setting up the discharge gate for the macroporous resin that washes can discharge.
Further, a second liquid inlet is fixedly connected to one side, far away from the first liquid inlet, of the outer side wall of the box body;
through above-mentioned technical scheme, through setting up the second inlet for the ethanol can get into the agitator tank.
Further, two observation windows are embedded in the front end face of the box body;
through the technical scheme, the device operation can be observed by arranging the observation window.
The utility model has the following beneficial effects:
1. according to the macroporous resin cleaning equipment for separating the fermentation products of the pichia pastoris, provided by the utility model, the macroporous resin is filled into the stirring tank through the feed inlet by arranging the feed inlet, the motor, the rotating rod, the stirring paddle and the stirring tank, the stirring paddle is driven to rotate by the motor, the ethanol in the stirring tank is driven, and the generated centrifugal force cleans the fermentation products in the macroporous resin, so that the macroporous resin is cleaned more cleanly.
2. According to the macroporous resin cleaning equipment for separating the fermentation product of the pichia pastoris, which is provided by the utility model, the macroporous resin which is stirred in the device is conveyed into the standing box through the material pump to enter ethanol liquid for standing by arranging the material sucking pipe, the material pump, the material discharging pipe and the standing box, so that residual fermentation materials are removed.
3. The macroporous resin cleaning equipment for separating the fermentation product of the pichia pastoris is provided with a cleaning water tank, a water guide pipe, a water pump, a water outlet, a supporting plate, a water diversion pipe, a spray head, a cleaning box, a filter tank, a liquid collecting tank, a second liquid inlet, a discharge port and a waste liquid outlet, wherein the cleaning water is transmitted to the water diversion pipe through the water guide pipe and the water pump through the cleaning water tank, and the spray head is used for flushing materials entering the filter tank, so that the macroporous resin can be automatically cleaned by the device.
Drawings
FIG. 1 is an isometric view of a macroporous resin cleaning apparatus for separating a fermentation product of Pichia pastoris according to the present utility model;
FIG. 2 is a front cross-sectional view of a macroporous resin cleaning apparatus for separating a fermentation product of Pichia pastoris according to the present utility model;
FIG. 3 is a rear view of a macroporous resin cleaning apparatus for separating a fermentation product of Pichia pastoris according to the present utility model;
FIG. 4 is a left side view of a macroporous resin cleaning apparatus for separating Pichia pastoris fermentation products according to the present utility model;
fig. 5 is an enlarged view at a in fig. 2.
Legend description:
1. a case; 2. an observation window; 3. a feed inlet; 4. a motor; 5. a rotating lever; 6. stirring paddles; 7. a stirring tank; 8. a suction pipe; 9. a material pump; 10. a discharge pipe; 11. standing the box; 12. a first liquid inlet; 13. cleaning the water tank; 14. a water conduit; 15. a water pump; 16. a water outlet; 17. a support plate; 18. a water diversion pipe; 19. a spray head; 20. a cleaning box; 21. a filter tank; 22. a liquid collecting tank; 23. a second liquid inlet; 24. a discharge port; 25. a waste liquid outlet; 26. and a discharge port.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-5, one embodiment provided by the present utility model is: the macroporous resin cleaning equipment for separating the fermentation products of the pichia pastoris comprises a box body 1, wherein one side of the box body 1 is provided with a feed inlet 3 in a penetrating way, one side of the upper end face of the box body 1 is fixedly provided with a motor 4, the output end of the motor 4 is fixedly connected with a rotating rod 5, one end of the rotating rod 5 is provided with the box body 1 and a stirring box 7 in a penetrating way and is fixedly connected with a plurality of stirring paddles 6, the macroporous resin is filled into the stirring box 7 through the feed inlet 3, the motor 4, the rotating rod 5, the stirring paddles 6 rotate, ethanol in the stirring box 7 is driven, generated centrifugal force cleans the fermentation products in the macroporous resin, the macroporous resin is cleaned more cleanly, the stirred macroporous resin is conveyed into ethanol solution through the stirring box 11 through the material pump 9, the discharging pipe 10 and the stirring box 11, and the residual fermentation materials are removed;
the lateral wall one side of agitator tank 7 is run through and is provided with the material pipe 8 that absorbs, the one end that the agitator tank 7 was kept away from to material pipe 8 is fixedly provided with material pump 9, the output fixedly connected with discharging pipe 10 of material pump 9, the one end that material pump 9 was kept away from to discharging pipe 10 runs through and is provided with the case 11 of standing, the up end fixedly connected with first inlet 12 of case 11 of standing, one side fixedly connected with bin outlet 26 of case 11 of standing, through setting up wash tank 13, aqueduct 14, water pump 15, delivery port 16, backup pad 17, distributive pipe 18, shower nozzle 19, wash tank 20, filter tank 21, collecting vat 22, second inlet 23, discharge gate 24 and waste liquid export 25, pass through wash tank 13 and pass through aqueduct 14 and water pump 15 to distributive pipe 18, wash the material that gets into filter tank 21 through shower nozzle 19 for the device can self-cleaning macroporous resin.
The up end of box 1 keeps away from motor 4's one side fixedly provided with wash tank 13, wash tank 13's lower terminal surface fixedly connected with aqueduct 14, aqueduct 14 keeps away from wash tank 13's one end fixedly provided with water pump 15, water pump 15's output fixedly connected with delivery port 16, water outlet 16's one end fixedly connected with shunt tube 18, shunt tube 18's lateral wall fixedly connected with a plurality of shower nozzles 19, make the device wash the macroporous resin that purifies voluntarily, make the device use more convenient, the manpower is saved, box 1's inside wall keeps away from one side fixedly provided with wash tank 20 of agitator tank 7, wash tank 20's inner wall fixedly connected with backup pad 17, water pump 15 is located backup pad 17, through setting up the catchment tank 22, make the waste liquid of washing can discharge through setting up shower nozzle 19, make the device wash tank 20's inside wall fixedly connected with the outlet 25, make the drain hole 25 enter the side of agitator tank 2, make the drain hole 2 through setting up the side of agitator tank 25, the side of the utility model is provided with the first drain window 2, the drain hole is provided with the drain hole 2, the drain hole is connected with the drain hole 2, the drain hole is provided with the drain hole 2 through setting up the drain hole 2, the drain hole is provided with the drain hole 2.
Working principle: when the device is used, macroporous resin is filled into the stirring tank 7 through the feed inlet 3, the rotating rod 5 is driven by the motor 4, the stirring paddle 6 is rotated, ethanol inside the stirring tank 7 is driven, generated centrifugal force cleans fermentation materials inside the macroporous resin, so that the macroporous resin is cleaned more cleanly, the stirred macroporous resin in the device is conveyed into the standing tank 11 through the material pump 9 to enter ethanol liquid for standing, residual fermentation materials are removed, cleaning water is conveyed to the water diversion pipe 18 through the cleaning water tank 13 through the water guide pipe 14 and the water pump 15, and the materials entering the filter tank 21 are washed through the spray head 19, so that the device can automatically clean the macroporous resin.
Finally, it should be noted that: the foregoing description is only illustrative of the preferred embodiments of the present utility model, and although the present utility model has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements or changes may be made without departing from the spirit and principles of the present utility model.
Claims (8)
1. The macroporous resin cleaning equipment for separating the fermentation product of pichia pastoris comprises a box body (1), and is characterized in that: one side of the box body (1) is provided with a feed inlet (3) in a penetrating way, one side of the upper end face of the box body (1) is fixedly provided with a motor (4), the output end of the motor (4) is fixedly connected with a rotating rod (5), and one end of the rotating rod (5) is provided with the box body (1) and a stirring box (7) in a penetrating way and is fixedly connected with a plurality of stirring paddles (6);
the stirring tank is characterized in that a suction pipe (8) is arranged on one side of the outer side wall of the stirring tank (7) in a penetrating mode, a material pump (9) is fixedly arranged at one end, far away from the stirring tank (7), of the suction pipe (8), a discharging pipe (10) is fixedly connected to the output end of the material pump (9), a standing tank (11) is arranged at one end, far away from the material pump (9), of the discharging pipe (10) in a penetrating mode, a first liquid inlet (12) is fixedly connected to the upper end face of the standing tank (11), and a discharge port (26) is fixedly connected to one side of the standing tank (11).
2. The macroporous resin cleaning apparatus for separating a fermentation product of pichia pastoris according to claim 1, wherein: the utility model discloses a water distribution device for the water heater, including motor (4) and box (1), the up end of box (1) is kept away from one side of motor (4) fixedly provided with wash water tank (13), the lower terminal surface fixedly connected with aqueduct (14) of wash water tank (13), the one end that washs water tank (13) was kept away from to aqueduct (14) is fixedly provided with water pump (15), the output fixedly connected with delivery port (16) of water pump (15), the one end fixedly connected with shunt tubes (18) of delivery port (16), lateral wall fixedly connected with shower nozzles (19) of shunt tubes (18).
3. A macroporous resin cleaning apparatus for separating pichia pastoris fermentation products according to claim 2, wherein: the inner side wall of the tank body (1) is fixedly provided with a cleaning tank (20) on one side far away from the stirring tank (7), the inner bottom end face of the cleaning tank (20) is fixedly provided with a liquid collecting tank (22), the inner side wall of the cleaning tank (20) is fixedly connected with a supporting plate (17), and the water pump (15) is located on the supporting plate (17).
4. A macroporous resin cleaning apparatus for separating a fermentation product of pichia pastoris according to claim 3, wherein: a filter tank (21) is fixedly arranged on one side of the inner side wall of the cleaning box (20), and the filter tank (21) is positioned below the spray heads (19).
5. A macroporous resin cleaning apparatus for separating a fermentation product of pichia pastoris according to claim 3, wherein: one side of the liquid collecting groove (22) is fixedly connected with a waste liquid outlet (25), and the waste liquid outlet (25) penetrates through the box body (1).
6. The macroporous resin cleaning apparatus for separating a fermentation product of pichia pastoris according to claim 4, wherein: one side of the filter tank (21) is fixedly connected with a discharge port (24).
7. The macroporous resin cleaning apparatus for separating a fermentation product of pichia pastoris according to claim 1, wherein: and one side, far away from the first liquid inlet (12), of the outer side wall of the box body (1) is fixedly connected with a second liquid inlet (23).
8. The macroporous resin cleaning apparatus for separating a fermentation product of pichia pastoris according to claim 1, wherein: the front end face of the box body (1) is embedded with two observation windows (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321696520.1U CN220160729U (en) | 2023-06-30 | 2023-06-30 | Macroporous resin cleaning equipment for separating pichia pastoris fermentation products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321696520.1U CN220160729U (en) | 2023-06-30 | 2023-06-30 | Macroporous resin cleaning equipment for separating pichia pastoris fermentation products |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220160729U true CN220160729U (en) | 2023-12-12 |
Family
ID=89066485
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321696520.1U Active CN220160729U (en) | 2023-06-30 | 2023-06-30 | Macroporous resin cleaning equipment for separating pichia pastoris fermentation products |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220160729U (en) |
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2023
- 2023-06-30 CN CN202321696520.1U patent/CN220160729U/en active Active
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