CN220116535U - Cell brush for cell extraction - Google Patents

Abstract

The utility model discloses a cell brush for cell extraction, which comprises a pulling plate, a connecting piece, a brush head and a sleeve, wherein the connecting piece is used for connecting the brush head and the pulling plate; the brush head comprises a folding part and an elastic supporting part, wherein the folding part is folded towards one end far away from the connecting piece by taking the connecting end of the folding part and the connecting piece as a folding point. Cell brush for cell extraction is through setting up folding brush head to support the folding portion of brush head through elastic support portion, before scraping the cell, through with the brush head folding income sleeve in, make things convenient for the brush head to get into the cell culture bottle that the bottleneck bore is less, after putting into cell culture bottle with the brush head after folding, expand the brush head and scrape the cell, after accomplishing scraping of cell, repeatedly the folding action of brush head before, and can scrape folding portion and telescopic inner wall, scrape the overhead remaining cell of brush off, make the cell sap drop into the culture bottle along telescopic bottom, in order to avoid unnecessary loss.

Description

Cell brush for cell extraction

Technical Field

The utility model relates to a cell brush for cell extraction, and belongs to the technical field of cell extraction.

Background

Western Blot (WB) is a major difficulty in biological experiments, and the precondition for preparing WB is that a good protein sample is prepared, and cells to be scraped and lysed are required to be scraped by a cell scraper in the process of total cell protein extraction experiments;

the existing cell scraper has the following inconvenience and problems: 1. the scraper is too small, and the scraper needs to be scraped for many times, so that time is wasted. 2. The caliber of the cell culture bottle is small, the movable range of the existing scraper is small, and the operation is inconvenient. 3. After scraping the cells, residual cells are left on the cell scraper, so that waste is caused.

It can be seen that there is a need for a cytobrush for cell extraction in order to save scraping time and reduce cell loss during the scraping of lysed cells.

Disclosure of Invention

The utility model aims to overcome the defects in the prior art, and provides a cell brush for cell extraction, which is characterized in that a foldable brush head is arranged, and a folding part of the brush head is supported by an elastic supporting part, before cell scraping is carried out, the brush head is folded into a sleeve, so that the brush head can conveniently enter a cell culture bottle with smaller caliber of a bottle mouth, after the folded brush head is put into the cell culture bottle, the brush head is unfolded to scrape cells, after the scraping of the cells is finished, the folding action of the brush head is repeated, in addition, in the folding process, the folding part and the inner wall of the sleeve are scraped, and residual cells on the brush head are scraped off, so that cell liquid is dripped into the culture bottle along the bottom of the sleeve, and unnecessary loss is avoided.

In order to achieve the above purpose/solve the above technical problems, the present utility model is realized by adopting the following technical scheme: a cell brush for cell extraction comprises a pull plate, a connecting piece, a brush head and a sleeve, wherein,

the connecting piece is used for connecting the brush head and the pulling plate;

the brush head comprises a folding part and an elastic supporting part, wherein the folding part is folded towards one end far away from the connecting piece by taking the connecting end of the folding part and the connecting piece as folding points, and the elastic supporting part is respectively fixed with the folding part and the connecting piece and is used for resetting the folding part under the condition of no external force limitation;

the sleeve is sleeved on the outer side of the connecting piece, and when the brush head is pulled to move towards the sleeve through the pulling plate, the brush head is folded under the action of the sleeve and is retracted into the sleeve.

Further, the elastic supporting portion comprises a spring piece.

Further, the fixing piece is used for fixing the sleeve and the bottle mouth of the cell culture bottle.

Further, the fixing member includes a clip.

Further, the diameter of the sleeve is much smaller than the length of the fold.

Further, the diameter of the sleeve is slightly larger than the width of the folded portion.

Further, the connector comprises a telescopic column.

Further, the connector comprises a connecting post.

Compared with the prior art, the utility model has the beneficial effects that:

according to the cell brush for cell extraction, the foldable brush head is arranged, the folding part of the brush head is supported by the elastic supporting part, before cell scraping is carried out, the brush head is folded into the sleeve, so that the brush head can conveniently enter a cell culture bottle with smaller caliber of a bottle mouth, after the folded brush head is put into the cell culture bottle, the brush head is unfolded for cell scraping, after cell scraping is completed, the folding action of the brush head before the process of cell scraping is repeated, the folding part and the inner wall of the sleeve can be scraped in the folding process, and residual cells on the brush head are scraped, so that cell liquid is dripped into the culture bottle along the bottom of the sleeve, and unnecessary loss is avoided;

according to the cell brush for cell extraction, provided by the utility model, the fixing piece for fixing the sleeve and the bottle mouth of the cell culture bottle is arranged, so that the use of operators can be facilitated, and the scraping efficiency is improved.

Drawings

FIG. 1 is a schematic diagram of a cell brush for cell extraction according to an embodiment;

FIG. 2 is a schematic top view of the structure of the cytobrush of FIG. 1;

FIG. 3 is a schematic side view of the structure of the cytobrush of FIG. 1;

FIG. 4 is a schematic view of the brush head of the cellular brush of FIG. 1 in a folded state;

FIG. 5 is a schematic illustration of the engagement of the cytobrush of FIG. 1 with a cell culture flask.

In the figure: 1. pulling a plate; 2. a connecting piece; 3. a brush head; 4. a sleeve; 5. a folding part; 6. an elastic support part; 7. and a fixing piece.

Detailed Description

The utility model is further described below with reference to the accompanying drawings. The following examples are only for more clearly illustrating the technical aspects of the present utility model, and are not intended to limit the scope of the present utility model.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", etc. may explicitly or implicitly include one or more such feature. In the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art in a specific case.

Embodiment one:

as shown in fig. 1 to 4, the present embodiment provides a cytobrush for cell extraction, comprising a pulling plate 1, a connecting member 2, a brush head 3, a sleeve 4, wherein,

the connecting piece 2 is used for connecting the brush head 3 and the pull plate 1;

the brush head 3 comprises a folding part 5 and an elastic supporting part 6, wherein the folding part 5 is folded towards one end far away from the connecting piece 2 by taking the connecting end of the folding part 5 and the connecting piece 2 as a folding point, and the elastic supporting part 6 is respectively fixed with the folding part 5 and the connecting piece 2 and is used for resetting the folding part 5 under the condition of no external force limitation;

the sleeve 4 is sleeved on the outer side of the connecting piece 2, and when the brush head 3 is pulled by the pull plate 1 to move towards the sleeve 4, the brush head 3 is folded under the action of the sleeve 4 and is retracted into the sleeve 4.

In the above technical solution, by arranging the foldable brush head 3 and supporting the folded portion 5 of the brush head 3 through the elastic supporting portion 6, before scraping cells, the brush head 3 is pulled by the pulling plate 1 to move towards the sleeve 4, so that the brush head 3 is folded under the action of the sleeve 4 and is retracted into the sleeve 4, as shown in fig. 4, at this time, the folded brush head 3 can conveniently enter the cell culture flask a with smaller bottleneck caliber, after the folded brush head 3 is placed into the cell culture flask a, the pulling plate 1 is pushed to move the brush head 3 towards a direction far away from the sleeve 4, after the restriction of the sleeve 4 is not met, the folded portion 5 of the brush head 3 is reset and unfolded under the action of the elastic supporting portion 6, at this time, the scraping of cells can be performed, after the scraping of the cells is completed, the folded portion 5 and the inner wall of the sleeve 4 can be scraped, and the residual cells on the brush head 3 can be scraped in the folding process, so that the cell liquid is dripped into the bottom of the sleeve 4, and unnecessary loss is avoided;

it should be noted that, in order to ensure smooth folding and unfolding of the brush head 3, it is necessary for an operator to hold the sleeve 4 with one hand and hold the pulling plate 1 with one hand.

In some embodiments, to enable the elastic support portion 6 to reset the folded portion 5, the elastic support portion 6 may use a spring; however, the elastic supporting portion 6 is not limited thereto, and other structures may be adopted as long as the supporting and resetting of the folding portion 5 can be realized, for example: torsion springs, etc.

In some embodiments, as shown in fig. 5, for the convenience of the operator, the fixing member 7 is further included, where the fixing member 7 is used to fix the sleeve 4 and the mouth of the cell culture flask a.

In some embodiments, to fix the sleeve 4 to the mouth of the cell culture flask a by the fixing member 7, a clip may be used for the fixing member 7; however, the fixing member 7 is not limited thereto, and other structures may be adopted as long as the fixation of the sleeve 4 to the mouth of the cell culture flask a can be achieved, for example: the clamping plates are fixed in a mode of being matched with the bolts.

In some embodiments, the diameter of the sleeve 4 is much smaller than the length of the fold 5 in order to ensure that the brush head 3 has a sufficient length to achieve a wide range of scraping.

In some embodiments, in order to ensure that the brush head 3 can be smoothly retracted into the sleeve 4 and scraped with the sleeve 4 to achieve secondary scraping, the diameter of the sleeve 4 is slightly larger than the width of the folded portion 5.

In some embodiments, the connector 2 may employ a telescopic column to ensure easy storage and adjustability of the whole cytobrush.

In some embodiments, the connecting piece 2 may also use a connecting post to achieve connection and fixation between the brush head 3 and the pulling plate 1, and at the same time, ensure stability during cell scraping.

In summary, according to the cellular brush for cell extraction provided in this embodiment, by providing the foldable brush head 3 and supporting the folded portion 5 of the brush head 3 through the elastic supporting portion 6, before scraping cells, the brush head 3 is folded into the sleeve 4, so that the brush head 3 can conveniently enter the cell culture bottle a with smaller aperture of the bottle mouth, after the folded brush head 3 is placed into the cell culture bottle a, the brush head 3 is unfolded to scrape cells, after scraping of cells is completed, the folding action of the brush head 3 is repeated, and in the folding process, the folded portion 5 and the inner wall of the sleeve 4 can be scraped, and the residual cells on the brush head 3 are scraped, so that the cell liquid drops into the culture bottle along the bottom of the sleeve 4, so as to avoid unnecessary loss; through setting up the mounting 7 that is used for fixed sleeve 4 and cell culture bottle A bottleneck, can be convenient for operating personnel's use, improve scraping efficiency.

The foregoing is merely a preferred embodiment of the present utility model, and it should be noted that modifications and variations could be made by those skilled in the art without departing from the technical principles of the present utility model, and such modifications and variations should also be regarded as being within the scope of the utility model.

Claims (8)

1. A cell brush for cell extraction is characterized by comprising a pull plate (1), a connecting piece (2), a brush head (3) and a sleeve (4), wherein,

the connecting piece (2) is used for connecting the brush head (3) and the pull plate (1);

the brush head (3) comprises a folding part (5) and an elastic supporting part (6), wherein the folding part (5) is folded towards one end far away from the connecting piece (2) by taking the connecting end of the folding part and the connecting piece (2) as a folding point, and the elastic supporting part (6) is respectively fixed with the folding part (5) and the connecting piece (2) and is used for resetting the folding part (5) under the condition of no external force limitation;

the sleeve (4) is sleeved on the outer side of the connecting piece (2), and when the brush head (3) is pulled towards the sleeve (4) through the pull plate (1), the brush head (3) is folded under the action of the sleeve (4) and is retracted into the sleeve (4).

2. The cytobrush for cell extraction as claimed in claim 1, wherein said elastic support (6) comprises a shrapnel.

3. The cytobrush for cell extraction as claimed in claim 1, further comprising a fixing member (7), said fixing member (7) being for fixing the sleeve (4) and the mouth of the cell culture flask.

4. A cytobrush for cell extraction according to claim 3, characterized in that the fixture (7) comprises a clip.

5. A cytobrush for cell extraction according to claim 1, characterized in that the diameter of the sleeve (4) is much smaller than the length of the fold (5).

6. A cytobrush for cell extraction according to claim 1, characterized in that the diameter of the sleeve (4) is slightly larger than the width of the fold (5).

7. A cytobrush for cell extraction according to claim 1, characterized in that the connector (2) comprises a telescopic column.

8. A cytobrush for cell extraction according to claim 1, characterized in that the connector (2) comprises a connecting post.