CN220116529U

CN220116529U - Phage filtering and diluting device

Info

Publication number: CN220116529U
Application number: CN202321632034.3U
Authority: CN
Inventors: 吴洪星
Original assignee: Qingdao Yuanda Fisheries Technology Development Co ltd
Current assignee: Qingdao Yuanda Fisheries Technology Development Co ltd
Priority date: 2023-06-26
Filing date: 2023-06-26
Publication date: 2023-12-01
Anticipated expiration: 2033-06-26

Abstract

The utility model relates to the technical field of biochemical experiment tools, in particular to a filtering dilution device for phages, which comprises a base, wherein a placing plate is connected onto the base in a sliding manner, a pressure sensor is fixedly connected between the bottom of the placing plate and the inner wall of the bottom of the base, and the filtering device comprises a filtering funnel and filter paper.

Description

Phage filtering and diluting device

Technical Field

The utility model relates to the technical field of biochemical experiment tools, in particular to a filtering and diluting device for phage.

Background

Phage is a virus capable of phagocytizing bacteria, and is often used in research in the biochemical field due to phagocytosis of bacteria, for example, phage may be used in the medical industry to kill bacteria which cannot be effectively inhibited by drugs at present through RNA modification.

In the process of research, the concentration of the phage is also one of important indicators, so that the sterilizing effect of the phage at different concentrations needs to be observed by properly diluting the concentration of the culture solution of the phage, while the concentration of the culture solution of the phage needs to be adjusted by chemical tools such as a rubber head dropper, and the volume of the culture solution added needs to be judged by naked eyes, so that the problem of large error is likely to occur, and a more accurate dilution device is needed to dilute the culture solution of the phage.

Disclosure of Invention

The utility model aims to provide a filtering and diluting device for phage, which is used for solving the problems in the background technology.

The technical scheme of the utility model is as follows: the filtering and diluting device for the bacteriophage comprises a base, wherein a placing plate is connected to the base in a sliding manner, and a pressure sensor is fixedly connected between the bottom of the placing plate and the inner wall of the bottom of the base;

the filter assembly comprises a filter funnel and filter paper, the filter funnel is arranged above the base, and the filter paper is fixedly connected to the inside of the filter funnel;

the auxiliary assembly comprises a support and an inserting block, wherein the base is fixedly connected with a connecting rod, the support is fixedly connected to the connecting rod, the inserting block is fixedly connected to the filtering funnel, and the inserting block is in sliding connection with the support.

Preferably, the base is fixedly connected with a display screen, and the display screen is electrically connected with the pressure sensor.

Preferably, the filter assembly further comprises a bottom tube fixedly connected to the bottom of the filter funnel.

Preferably, the auxiliary assembly further comprises a slot, the slot is arranged on the support, and the insert block is connected to the inside of the slot in a sliding mode.

Preferably, the auxiliary assembly further comprises a rubber sheet, the rubber sheet is fixedly connected to the inside of the filter funnel, and the rubber sheet is close to the top end of the filter funnel.

Preferably, the auxiliary assembly further comprises a bottom seal fixedly connected to the bottom of the bottom pipe.

The utility model provides a filtering and diluting device for phage through improvement, which has the following improvements and advantages compared with the prior art:

the method comprises the following steps: in the utility model, an unused culture dish is placed on a placing plate, the mass of the culture dish is applied to a pressure sensor through the placing plate, the mass of the culture dish is displayed on a display screen, then the culture dish for culturing phage is placed on the placing plate, the mass is displayed through the display screen again, the difference value of the two data is the mass of phage culture solution on the culture dish, at the moment, the culture solution without phage is dripped into the culture dish through a filter funnel to dilute phage in the culture dish, the mass of the culture solution required to be dripped can be calculated according to the concentration required to be prepared, when the mass displayed on the display screen reaches the value, the filter funnel can be detached, and the dripping is stopped, and the concentration of phage culture solution in the culture dish reaches the target concentration;

and two,: according to the utility model, the filter assembly and the auxiliary assembly are arranged, so that the filter funnel can be placed on the support, the insert on the filter funnel is inserted into the slot on the support to fix the filter funnel, at the moment, the culture solution without bacteriophage is injected into the filter funnel through the injector, the needle tube of the injector is required to pass through the rubber sheet during injection, so that the culture solution without bacteriophage is injected into the filter funnel, the rubber sheet is arranged to ensure sterility of the filter funnel before use, after the culture solution is injected into the filter funnel, the bottom seal at the bottom of the bottom tube is torn off, the culture solution in the filter funnel can be slowly dropped down through the filter paper, compared with the manual dropping of the culture solution by using the rubber head dropper, more stable dropping frequency and single dropping amount can be ensured by using the filter funnel and the filter paper, the culture solution dropped down from the bottom of the filter funnel can pass through the bottom tube and then be directly dropped into the culture dish, dust in air adhered to the culture solution dropped down is reduced, the culture solution is gradually added into the culture dish, the quality of the culture dish is increased, and the culture dish is continuously broken, and the culture solution passes through the display screen.

Drawings

The utility model is further explained below with reference to the drawings and examples:

FIG. 1 is a schematic perspective view of the present utility model;

FIG. 2 is a bottom view of the utility model shown in FIG. 1;

fig. 3 is a cross-sectional view of the internal structure of the present utility model.

Reference numerals illustrate:

1. a base; 2. placing a plate; 3. a pressure sensor; 4. a display screen; 5. a connecting rod; 6. a filter assembly; 61. a filter funnel; 62. a filter paper; 63. a bottom tube; 7. an auxiliary component; 71. a rubber sheet; 72. a bottom seal; 73. a bracket; 74. a slot; 75. and (5) inserting blocks.

Detailed Description

The following detailed description of the present utility model clearly and fully describes the technical solutions of the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The utility model provides a filtering and diluting device for phage through improvement, and the technical scheme of the utility model is as follows:

1-3, a filtering and diluting device for phage comprises a base 1, wherein a placing plate 2 is connected to the base 1 in a sliding manner, a pressure sensor 3 is fixedly connected between the bottom of the placing plate 2 and the inner wall of the bottom of the base 1, and the device further comprises;

the filter assembly 6, the filter assembly 6 includes filter funnel 61 and filter paper 62, the filter funnel 61 is set up in the top of the base 1, the filter paper 62 is fixedly connected to the inside of the filter funnel 61, compared with using the rubber head drip tube to drip the culture solution manually, use filter funnel 61 and filter paper 62 can guarantee more stable drip frequency and single drip quantity;

the auxiliary assembly 7, the auxiliary assembly 7 includes support 73 and insert 75, fixedly connected with connecting rod 5 on the base 1, support 73 fixedly connected with connecting rod 5 is last, insert 75 fixed connection is on filter funnel 61, insert 75 and support 73 sliding connection.

Further, a display screen 4 is fixedly connected to the base 1, and the display screen 4 is electrically connected with the pressure sensor 3.

Further, the filter assembly 6 further comprises a bottom tube 63, and the bottom tube 63 is fixedly connected to the bottom of the filter funnel 61.

Further, the auxiliary assembly 7 further comprises a slot 74, the slot 74 is formed in the bracket 73, the insert 75 is slidably connected to the inside of the slot 74, the filter funnel 61 can be fixed by inserting the insert 75 on the filter funnel 61 into the slot 74 on the bracket 73, and the filter funnel 61 is disposable and can be removed and replaced after being used.

Further, the auxiliary assembly 7 further comprises a rubber sheet 71, the rubber sheet 71 is fixedly connected to the inside of the filter funnel 61, the rubber sheet 71 is close to the top end of the filter funnel 61, and the rubber sheet 71 is arranged so that the inside of the filter funnel 61 can be ensured to be sterile before use.

Further, the auxiliary assembly 7 further comprises a bottom seal 72, wherein the bottom seal 72 is fixedly connected to the bottom of the bottom tube 63, and the bottom seal 72 is matched with the rubber sheet 71 on the filter funnel 61 before being released, so that the filter funnel 61 and the bottom tube 63 can be ensured to be sterile and not to be in contact with external air.

Working principle: firstly, an unused culture dish is placed on the placing plate 2, the mass of the culture dish is applied on the pressure sensor 3 through the placing plate 2, and the mass of the culture dish is displayed on the display screen 4, then, the culture dish for culturing phage is placed on the placing plate 2, and the mass is displayed again through the display screen 4, so that the difference between the two data is the mass of phage culture liquid on the culture dish, at the moment, one filter funnel 61 is placed on the bracket 73, the filter funnel 61 is fixed by inserting an inserting block 75 on the filter funnel 61 into a slot 74 on the bracket 73, at the moment, the culture liquid without phage is injected into the filter funnel 61 through an injector, at the moment of injection, the needle tube of the injector needs to pass through the rubber sheet 71, so that the culture liquid without phage is injected into the filter funnel 61, the rubber sheet 71 is arranged so that the filter funnel 61 can ensure the internal sterility before use, when the culture solution is injected into the filter funnel 61, the bottom seal 72 at the bottom of the bottom tube 63 is torn off, the culture solution in the filter funnel 61 can be slowly dropped downwards through the filter paper 62, compared with the manual dropping of the culture solution by using a rubber head dropper, the filter funnel 61 and the filter paper 62 can ensure more stable dropping frequency and single dropping amount, the culture solution dropped from the bottom of the filter funnel 61 can pass through the bottom tube 63 and then be directly dropped into a culture dish, dust in air adhered by the downward dropped culture solution is reduced, the culture solution is gradually added into the culture dish, the mass in the culture dish is continuously increased, the culture solution is sensed by the pressure sensor 3, the display screen 4 displays that the mass of the culture solution with bacteriophage is calculated in advance, so the mass of the culture solution required to be dropped can be calculated according to the concentration required to be prepared, when the quality displayed on the display screen 4 reaches the value, the filter funnel 61 is removed, and the dripping is stopped, so that the concentration of the phage culture solution in the culture dish reaches the target concentration.

Claims

1. The utility model provides a phage is with filtering diluting device, includes base (1), sliding connection has on base (1) to place board (2), places fixedly connected with pressure sensor (3) between the bottom of board (2) and the bottom inner wall of base (1), its characterized in that: also comprises;

the filter assembly (6), the filter assembly (6) comprises a filter funnel (61) and filter paper (62), the filter funnel (61) is arranged above the base (1), and the filter paper (62) is fixedly connected to the inside of the filter funnel (61);

the auxiliary assembly (7), auxiliary assembly (7) include support (73) and inserted block (75), fixedly connected with connecting rod (5) on base (1), support (73) fixedly connected with is on connecting rod (5), inserted block (75) fixed connection is on filter funnel (61), and inserted block (75) and support (73) sliding connection.

2. The phage filter-dilution apparatus of claim 1, wherein: the base (1) is fixedly connected with a display screen (4), and the display screen (4) is electrically connected with the pressure sensor (3).

3. The phage filter-dilution apparatus of claim 1, wherein: the filter assembly (6) further comprises a bottom pipe (63), and the bottom pipe (63) is fixedly connected to the bottom of the filter funnel (61).

4. The phage filter-dilution apparatus of claim 1, wherein: the auxiliary assembly (7) further comprises a slot (74), the slot (74) is arranged on the support (73), and the insert block (75) is connected inside the slot (74) in a sliding mode.

5. The phage filter-dilution apparatus of claim 1, wherein: the auxiliary assembly (7) further comprises a rubber sheet (71), the rubber sheet (71) is fixedly connected to the inside of the filter funnel (61), and the rubber sheet (71) is close to the top end of the filter funnel (61).

6. A phage filtration dilution apparatus according to claim 3, wherein: the auxiliary assembly (7) further comprises a bottom seal (72), and the bottom seal (72) is fixedly connected to the bottom of the bottom pipe (63).