CN220104862U - Turbidimetry dilution card - Google Patents
Turbidimetry dilution card Download PDFInfo
- Publication number
- CN220104862U CN220104862U CN202321139251.9U CN202321139251U CN220104862U CN 220104862 U CN220104862 U CN 220104862U CN 202321139251 U CN202321139251 U CN 202321139251U CN 220104862 U CN220104862 U CN 220104862U
- Authority
- CN
- China
- Prior art keywords
- dilution
- turbidity
- suspension
- sample
- card
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000012895 dilution Substances 0.000 title claims abstract description 51
- 238000010790 dilution Methods 0.000 title claims abstract description 51
- 238000004879 turbidimetry Methods 0.000 title description 2
- 239000000725 suspension Substances 0.000 claims abstract description 103
- 230000002550 fecal effect Effects 0.000 claims abstract description 91
- 239000007788 liquid Substances 0.000 claims abstract description 43
- 239000012898 sample dilution Substances 0.000 claims abstract description 10
- 239000003085 diluting agent Substances 0.000 claims description 32
- 238000000034 method Methods 0.000 claims description 20
- 239000008280 blood Substances 0.000 claims description 5
- 210000004369 blood Anatomy 0.000 claims description 5
- 239000002689 soil Substances 0.000 claims description 5
- 210000002700 urine Anatomy 0.000 claims description 5
- 238000001514 detection method Methods 0.000 abstract description 24
- 239000003086 colorant Substances 0.000 abstract description 13
- 238000004043 dyeing Methods 0.000 abstract description 10
- 238000001556 precipitation Methods 0.000 abstract description 9
- 238000004458 analytical method Methods 0.000 abstract description 6
- 238000010186 staining Methods 0.000 abstract description 6
- 238000007865 diluting Methods 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 44
- 239000002245 particle Substances 0.000 description 22
- 238000012545 processing Methods 0.000 description 14
- 238000010586 diagram Methods 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 8
- 210000003608 fece Anatomy 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 230000003287 optical effect Effects 0.000 description 7
- 238000003113 dilution method Methods 0.000 description 6
- 239000010794 food waste Substances 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 235000013601 eggs Nutrition 0.000 description 3
- 230000003071 parasitic effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000004445 quantitative analysis Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000013517 stratification Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012468 concentrated sample Substances 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical group [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- DWCZIOOZPIDHAB-UHFFFAOYSA-L methyl green Chemical compound [Cl-].[Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC(=CC=1)[N+](C)(C)C)=C1C=CC(=[N+](C)C)C=C1 DWCZIOOZPIDHAB-UHFFFAOYSA-L 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- NZYCYASKVWSANA-UHFFFAOYSA-M new methylene blue Chemical compound [Cl-].CCNC1=C(C)C=C2N=C(C=C(C(NCC)=C3)C)C3=[S+]C2=C1 NZYCYASKVWSANA-UHFFFAOYSA-M 0.000 description 1
- 230000005693 optoelectronics Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- LLWJPGAKXJBKKA-UHFFFAOYSA-N victoria blue B Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC(=CC=1)N(C)C)=C(C=C1)C2=CC=CC=C2C1=[NH+]C1=CC=CC=C1 LLWJPGAKXJBKKA-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
In the fecal component detection system, a liquid coloring agent is added into the fecal suspension, and the fecal suspension is diluted and colored; adding the fecal suspension after dyeing into a bearing chip; waiting for precipitation and layering of the fecal suspension in the bearing chip; layering and photographing the fecal suspension after the precipitation layering to obtain an image; and (5) analyzing and identifying the image to find out the object to be detected. The turbidimetric dilution card comprises turbidity levels of the suspension liquid and images corresponding to each turbidity level; sample dilution ratios corresponding to each turbidity rating. Diluting and staining the fecal suspension by the reference turbidity card; the turbidimetric card comprises a turbidity grade and a sample loading amount corresponding to the turbidity grade. The liquid state coloring agent can complete dilution and dyeing at the same time, and improves the sample treatment efficiency. The reference turbidity card is used for dilution, so that the dilution consistency of the sample is improved. The top layer image and the bottom layer image can be simultaneously or respectively analyzed to obtain images with different characteristic tangible components, and the analysis efficiency is improved.
Description
Technical Field
The utility model belongs to the technical field of analysis of components in a sample suspension, and particularly relates to a method and a system for analyzing components in a fecal sample suspension based on microscopic images, and a method and a system for analyzing components added to a fecal sample based on turbidimetric analysis.
Background
The automatic operation of the detection of the formed components of the excrement can be realized, so that the efficiency of clinical detection is greatly improved, but the direction which can be improved exists at the same time. Firstly, the feces have various formation components, and the classification detection of various types of formation components of bacteria, cells, ova and food residues cannot be realized rapidly and accurately; in the prior art, the content of a specific object to be detected is usually detected only after the specific object to be detected is extracted and screened.
In the excrement suspension, the distribution positions of the detection objects are different, some of the detection objects float on the top layer, some of the detection objects float on the middle layer, and how to accurately find the detection objects, so that the detection objects can be accurately focused to grasp the detection objects is a great challenge.
The fecal detection is greatly affected by the concentration of the sample, and the result accuracy can be affected by an excessively concentrated sample or an excessively diluted sample, so that the follow-up detection cannot realize the quantitative analysis of the target to-be-detected object in the fecal sample. If the dilution degree of the fecal suspension is to be controlled, very complicated weighing and proportioning steps are required, the control device and method are complex, the cost of the instrument is high, and the cost performance of fecal detection is reduced. The utility model hopes to improve the efficiency, accuracy and cost performance of fecal detection by optimizing the detection step.
Digital microscopes are monitors that use the anamorphic optics of a conventional optical microscope and images output by a digital camera, sometimes by software running on a computer. Digital microscopes typically have their own built-in LED light source, unlike optical microscopes in that there is no provision for directly viewing the sample through an eyepiece. Since the image is focused on the digital circuit, the entire system is designed for the monitor image. The optical elements of the human eye are omitted. The essence of the photoelectric automatic focusing technology of the optical microscope is the integration of optoelectronics technology, laser technology, computer image processing technology, automatic control and transmission technology, and also the result of the intelligent and automatic requirements of the optical microscope, and the photoelectric automatic focusing technology has the advantages of quick response and accuracy; the definition of the microscope image can be dynamically improved in real time.
The automatic focusing technology comprises two parts of axial positioning (focusing) and servo movement, and the axial positioning is the core. The essence of defocus is that the microscope object distance is not aligned or the jitter of the observed surface produces a change in object distance. To implement automatic focusing, defocus detection is firstly utilized to rapidly and dynamically perform defocus identification, and the defocus amount is automatically compensated by controlling the dynamic real-time rapid response of an actuating mechanism of a servo system through defocus signals. The common detection method for realizing defocus of the modern optical instrument comprises two steps: firstly, processing a video image by a computer to acquire defocusing information; and secondly, measuring the object distance by a photoelectric method, and extracting a defocusing signal.
According to the utility model, the size of the tested person can be obtained by measuring the size in the image under the condition that the eye distance of the digital microscope is fixed and the focusing is accurate and the magnification of the digital microscope is fixed. The focusing in the utility model refers to adjusting the object distance of a microscope under the condition of fixed eye distance, so as to obtain a clear image of an observed object.
Noun interpretation: suspension definition: small insoluble solid particles greater than 100 nanometers are suspended in a liquid to form a mixture.
Disclosure of Invention
In order to solve the problems that the detection efficiency of various different feces formed components is low and the feces can be effectively identified after being extracted or treated respectively in the prior art, the dilution consistency of a feces sample can be well controlled through a turbidimetric dilution card and a turbidimetric dilution method, and a foundation is laid for subsequent quantitative analysis.
The technical scheme for solving the technical problems is that the turbidimetric dilution card comprises turbidity grades of suspension liquid and images corresponding to each turbidity grade; also comprises any one or more of the following technical characteristics: the sample dilution ratio corresponding to the turbidity grade is also included; adding corresponding diluent into the fecal suspension according to the sample dilution ratio; the method also comprises the sample adding amount of the diluent corresponding to the turbidity grade; adding corresponding diluent to the fecal suspension with the set volume according to the sample adding amount of the diluent; the sample addition amount corresponding to the turbidity grade is also included; and adding the corresponding fecal suspension into the diluent with the set volume according to the sample addition amount.
The sample adding amount of the diluent is increased according to the turbidity grade, and the sample adding amount of the diluent is increased by a set value of the liquid adding amount every time one turbidity grade is increased.
The image corresponding to each turbidity level comprises a gray-scale image or a color image.
The dilution liquid sample adding amount is increased according to the turbidity grade, and the dilution liquid sample adding amount is increased by one order of magnitude when one turbidity grade is increased.
The turbidimetric dilution card comprises a colorimetric area, wherein the colorimetric area is positioned above an image, and the colorimetric area is white.
The suspension comprises any one of blood, urine, fecal suspension and soil suspension.
The technical effects of the technical scheme are as follows: the reference turbidity card is used for dilution and dyeing, the dyeing dilution ratio can be well controlled, the dilution consistency of the sample is improved, and a foundation is laid for subsequent quantitative analysis and calculation.
The technical effects of the technical scheme are as follows: according to the turbidity levels of the suspension, visually comparing images corresponding to each turbidity level; the sample adding amount of the diluent corresponding to each turbidity level is intuitively and simply obtained, so that the dilution work becomes simple and controllable, and the operability is good.
The technical effects of the technical scheme are as follows: the sample adding amount of the diluent is increased according to the turbidity grade, and the sample adding amount of the diluent is increased by one liquid adding amount set value or one order of magnitude every time one turbidity grade is increased, so that different liquid adding amounts can be conveniently determined according to the turbidity grade.
The technical effects of the technical scheme are as follows: the images corresponding to each turbidity level comprise gray level images or color images, so that machine or manual comparison and identification are very convenient.
The technical effects of the technical scheme are as follows: the colorimetric area is positioned above the image, is white and is convenient for background comparison so as to carry out colorimetric verification.
The technical effects of the technical scheme are as follows: the suspension comprises any one of blood, urine, fecal suspension and soil suspension, and has wide application range.
The technical effects of the technical scheme are as follows: the suspension of the object to be detected is obtained by taking a quantitative liquid; the quantitative liquid taking amount of the suspension of the object to be detected can be different; turbidimetric dilution cards corresponding to different liquid sampling amounts can be arranged.
Drawings
FIG. 1 is a schematic block diagram of a method for detecting fecal components;
FIG. 2 is a schematic block diagram of a method for detecting fecal components;
FIG. 3 is a schematic block diagram of a method for detecting fecal components;
FIG. 4 is a schematic illustration of sedimentation stratification of fecal suspension in a carrier chip;
FIG. 5 is an image of various formed components in a fecal suspension;
FIG. 6 is a schematic diagram of a turbidimetric dilution card;
FIG. 7 is a schematic diagram of a turbidimetric dilution card;
FIG. 8 is a schematic block diagram of a turbidimetric dilution method;
FIG. 9 is a schematic block diagram of a turbidimetric dilution method;
FIG. 10 is a schematic block diagram of a fecal component detection system;
FIG. 11 is a schematic illustration of precipitation stratification of a fecal suspension containing reference particles in a carrier chip;
fig. 12 is a schematic diagram of a carrier chip.
Detailed Description
The present utility model is described in further detail below with reference to the accompanying drawings.
The following description of the preferred embodiments of the present utility model is not intended to limit the present utility model. The description of the preferred embodiments of the present utility model is merely illustrative of the general principles of the utility model. The embodiments described in this disclosure are only some, but not all, embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the present utility model, it should be understood that the terms "center," "longitudinal," "transverse," "length," "width," "thickness," "up," "down," "front," "back," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," etc. indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings are merely for convenience in describing the present utility model and to simplify the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and technical features numbered with numerals such as Arabic numerals 1, 2, 3, etc., and such numbers as "A" and "B" are used for descriptive purposes only and are not intended to represent a temporal or spatial sequential relationship for ease of illustration; and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, features defining "first", "second", and numbered with numerals 1, 2, 3, etc., may explicitly or implicitly include one or more such features. In the description of the present utility model, the meaning of "a number" is two or more, unless explicitly defined otherwise.
As shown in fig. 1 to 3, in an embodiment of a method for detecting a fecal component, a liquid coloring agent is added to a fecal suspension, and the fecal suspension is diluted and colored; adding the fecal suspension after dyeing into a bearing chip; waiting for precipitation and layering of the fecal suspension in the bearing chip; layering and photographing the fecal suspension after the precipitation layering to obtain an image; and analyzing and identifying the image to find out the object to be detected. The fecal suspension is obtained by pre-diluting feces with saline. The saline may be physiological saline, or saline of other concentration or density.
As shown in fig. 1 to 3, the method for detecting the fecal component includes any one or more of the following technical features: technical characteristics F1: photographing the top layer of the fecal suspension to obtain a top layer image, and analyzing the top layer image through an algorithm to find out an object to be detected floating on the top layer; technical characteristics F2: photographing the bottom layer of the fecal suspension to obtain a bottom layer image, analyzing the bottom layer image through an algorithm, and finding out bacteria precipitated to the bottom layer; technical characteristics F3: photographing the bottom layer of the fecal suspension to obtain a bottom layer image, analyzing the bottom layer image through an algorithm, and finding out cells precipitated to the bottom layer; technical characteristics F4: and photographing the middle layer of the fecal suspension to obtain images of each layer, and analyzing the images of each layer through an algorithm to find out the object to be detected. The algorithm is an AI algorithm which is trained to recognize eggs, bacteria, cells and/or food residues.
As shown in fig. 1 to 3, the method for detecting the fecal component includes any one or more of the following technical features: technical characteristics S1: searching the interface between the top layer floater of the fecal suspension and the medium on the top layer of the bearing chip in the image by shooting images with different object distances to find the top layer of the fecal suspension; technical characteristics S2: and searching the interface between the sediment of the bottom layer of the fecal suspension and the medium of the bottom layer of the bearing chip in the image by shooting images with different object distances to find the bottom layer of the fecal suspension.
FIG. 12 is a schematic diagram of a carrier chip, labeled 1210, feces suspension flattened area for acquiring feces suspension images; reference numeral 1230 in the figure is a fecal suspension injection port, and reference numeral 1210 is a vent for rapid spreading of fecal suspension in the flattened area.
As shown in fig. 4, the schematic diagram of precipitation and layering of the fecal suspension in the carrying chip comprises a liquid accommodating cavity to be detected, such as the outer frame in fig. 4, and the height of the liquid accommodating cavity to be detected is a height set value; the height setting value of the liquid accommodating cavity to be detected is 150um to 500um.
As shown in FIG. 4, food residues, bacteria, cells and parasite eggs can be seen after sedimentation and stratification of the fecal suspension. In fig. 4, food residues and parasitic ova float on the upper part of the fecal suspension, and bacteria and cells settle on the lower part of the fecal suspension. In practical application, food residues and parasitic ova can also be suspended in or sunk in the lower part of the fecal suspension according to different sizes and properties.
As shown in fig. 5, a picture of various physical components including parasitic eggs, bacteria, fungi, cells and food residues was observed in one stool sample.
As shown in fig. 6 to 7, an embodiment of a turbidimetric dilution card includes turbidity levels of a suspension, and an image corresponding to each turbidity level; also comprises any one or more of the following technical characteristics: technical characteristics BA1: the sample dilution ratio corresponding to the turbidity grade is also included; adding corresponding diluent into the fecal suspension according to the sample dilution ratio; technical characteristics BA2: the method also comprises the sample adding amount of the diluent corresponding to the turbidity grade; adding corresponding diluent to the fecal suspension with the set volume according to the sample adding amount of the diluent; technical characteristics BA3: the sample addition amount corresponding to the turbidity grade is also included; and adding the corresponding fecal suspension into the diluent with the set volume according to the sample addition amount.
In an embodiment of the turbidimetric dilution card shown in fig. 6, the diluent loading is increased according to the turbidity level, and the diluent loading is increased by a loading set value every time the turbidity level is increased. In FIG. 6, the amount of the diluent added is set to 100ul, i.e., 100ul of diluent added per one turbidity grade. The image corresponding to each turbidity level comprises a gray-scale image or a color image. The diluent can be simply diluted or can be liquid dyeing agent, and the dilution and dyeing are finished at the same time. The amount of sample to be added to the turbidimetric dilution card varies depending on the turbidimetric dilution card of different types, and the content of the sample to be added varies.
In an embodiment of a turbidimetric dilution card not shown in some of the figures, the technical features BA2 are as follows: the dilution liquid sample adding amount is increased according to the turbidity grade, and the dilution liquid sample adding amount is increased by one order of magnitude when one turbidity grade is increased.
In one embodiment of the turbidimetric dilution card, as shown in fig. 7, a colorimetric region is included, which is located above the image, the colorimetric region being white. The suspension comprises any one of blood, urine, feces suspension and soil suspension.
As shown in fig. 8 to 9, the fecal suspension is diluted and stained with reference to the turbidimetric card; comprising any one or more of the following technical characteristics: technical characteristics AA1: the turbidimetric card comprises a turbidity grade and a sample dilution ratio corresponding to the turbidity grade; adding a corresponding liquid coloring agent into the fecal suspension according to the sample dilution ratio; technical characteristics AA2: the turbidimetric card comprises a turbidity grade and a liquid state coloring agent sample loading quantity corresponding to the turbidity grade; adding a corresponding liquid coloring agent into the fecal suspension with the set volume according to the liquid coloring agent sample adding quantity; technical characteristics AA3: the turbidimetric card comprises a turbidity grade and a sample adding amount corresponding to the turbidity grade; and adding the corresponding fecal suspension into the liquid coloring agent with the set volume according to the sample addition amount. And searching the required volume of the dilution liquid by the reference turbidity card, and adding the corresponding dilution liquid, namely the liquid coloring agent, into the quantitative fecal suspension to complete dilution and dyeing. The suspension of the object to be detected can be pre-diluted before turbidimetric dilution; or may be a raw sample without pre-dilution. In fecal sample processing, sometimes the fecal sample that encounters diarrhea may be the original sample, without pre-dilution.
Referring to fig. 10, a fecal component detection system includes an image processing module, an image acquisition module, a layered focusing module, a carrier chip, and a slipway assembly; the image acquisition module comprises an image sensor and an optical amplifying assembly; the bearing chip comprises a liquid accommodating cavity to be detected, and the height of the liquid accommodating cavity to be detected is a height set value; the bearing chip can be detachably arranged on the sliding table component; the sliding table component can adjust the distance between the image sensor and the bearing chip; adding the fecal suspension into a liquid accommodating cavity to be detected in the bearing chip, and precipitating and layering the fecal suspension in the bearing chip; the layered focusing module controls the sliding table assembly to slide and controls the image sensor to obtain images of different precipitation layers; the image processing module analyzes and identifies the images to find out the object to be detected. The fecal suspension is obtained by diluting and dyeing with a liquid state dyeing agent.
In the embodiment of the fecal component detection system not shown in fig. 10 and the drawings, the technical feature H0 is included: the sliding table assembly comprises a Z-axis sliding table, and the Z-axis sliding table can drive the bearing chip to move in the Z-axis direction which is relatively vertical to the horizontal plane; the Z-axis sliding table is used for adjusting the distance between the image sensor and the bearing chip. Comprises the technical characteristics of H1: the sliding table assembly comprises an X-axis sliding table, and the X-axis sliding table can drive the bearing chip to move in the X-axis direction of the horizontal plane; technical characteristics H2: the sliding table assembly comprises a Y-axis sliding table, and the Y-axis sliding table can drive the bearing chip to move in the Y-axis direction of the horizontal plane.
As shown in fig. 11, the liquid colorant includes reference particles, and the reference particles can assist focusing during image capturing. As shown in fig. 11, the method for detecting the fecal component includes any one or more of the following technical features: technical characteristics C1: the reference particles float on the top layer of the fecal suspension, and the reference particles assist the camera in shooting the top layer image; technical characteristics C2: the reference particles are deposited on the bottom layer of the fecal suspension, and the reference particles assist the camera to shoot the bottom layer image; technical characteristics C3: the reference particles include: and the reference particles float on the top layer, the reference particles precipitate on the bottom layer, and the measurement height of the liquid containing cavity to be detected of the bearing chip is obtained through photographing the reference particles on the top layer and the bottom layer.
As shown in fig. 8 to 11, in the embodiment of the fecal component detection system, the height of the detection liquid containing cavity of the carrying chip is set to 150um to 500um. Technical characteristics F1: the layered focusing module controls the sliding table assembly to slide, so that a clear image of the top layer of the fecal suspension is obtained; the image processing module analyzes the top layer image through an algorithm to find out the tangible components floating on the top layer; technical characteristics F2: the layered focusing module controls the sliding table assembly to slide, so that a clear image of the bottom layer of the fecal suspension is obtained; the image processing module analyzes the bottom layer image through an algorithm to find out bacteria deposited on the bottom layer; technical characteristics F3: the layered focusing module controls the sliding table assembly to slide, so that a clear image of the bottom layer of the fecal suspension is obtained; the image processing module analyzes the bottom layer image through an algorithm to find out cells precipitated on the bottom layer; technical characteristics F4: the layered focusing module controls the sliding table assembly to slide, so that a clear image of the bottom layer of the fecal suspension is obtained; lifting the focal plane by 5-20 um to obtain a fecal suspension image, and analyzing the image by an image processing module through an algorithm to find out bacteria deposited on the bottom layer.
As shown in fig. 8 to 11, in the embodiment of the fecal component detection system, any one or more of the following technical features are included: technical characteristics S1: the layered focusing module controls the sliding table assembly to slide, the image acquisition module shoots images with different object distances in the sliding process, and the image processing module searches the interface between the floating objects on the top layer of the fecal suspension and the medium on the top layer of the bearing chip in the images to find the top layer of the fecal suspension; technical characteristics S2: the layered focusing module controls the sliding table assembly to slide, the image acquisition module shoots images with different object distances in the sliding process, and the image processing module searches the interface between the sediment of the bottom layer of the fecal suspension and the medium of the bottom layer of the bearing chip in the images to find the bottom layer of the fecal suspension.
As shown in fig. 8 to 11, the fecal suspension includes reference particles that can assist in focusing during image capturing. Comprising any one or more of the following technical characteristics: technical characteristics C1: the image processing module finds out the reference particles in the images, and the images of the reference particles are found to be top-layer images; technical characteristics C2: the image processing module finds out the reference particles in the images, and the images of the reference particles are found as bottom images; technical characteristics C3: the reference particles include: the method comprises the steps that reference particles floating on the top layer and reference particles deposited on the bottom layer are separated, a layered focusing module controls a sliding table assembly to slide, an image acquisition module shoots images with different object distances in the sliding process, an image processing module finds out the reference particles floating on the top layer and the reference particles deposited on the bottom layer in the images, and the layered focusing module calculates and obtains the measurement height of a liquid accommodating cavity to be detected of a bearing chip through imaging distance difference between the top layer and the bottom layer.
Referring to fig. 8 to 9, in an embodiment of a turbidimetric dilution method, a reference turbidimetric card is used to dilute a suspension of a sample to be measured by adding a diluent; the turbidimetric card comprises the turbidity grade of the suspension of the object to be detected and the sample adding amount of the diluent corresponding to the turbidity grade. The suspension of the object to be detected is obtained by pre-diluting the object to be detected with water. The suspension of the object to be detected is diluted in a sample dilution ratio for fixing the same turbidity. In the suspension of the object to be detected in each turbidity level, the sample: the proportion range of the diluent is as follows; 50:1000, to 1000:2000. Sample: the ratio of the diluents may range from 50:1000, 100:1000, 200:1000, 300:1000, 400:1000, 500:1000, 600:1000, 700:1000, 1000:1000, 2000:1000. In the suspension of the object to be detected of each turbidity level, a corresponding dilution ratio range can be set according to the actual use requirement.
The turbidimetric dilution method can be used for turbidimetric dilution of different target analysis samples, so that different samples have the same quantitative dilution multiple. The turbidimetric dilution method can also be used for turbidimetric dilution of different turbidimetric values of the same target analysis sample, and morphological characteristics of the same target sample under different turbidimetric values can be obtained.
The diluent comprises a coloring agent, and the object to be detected is any one of blood, urine, fecal suspension and soil suspension. Also comprises the technical characteristics C1: the object to be detected is fecal suspension, and the staining agent comprises new methylene blue; technical characteristics C2: the to-be-detected object is fecal suspension, the staining agent comprises an acid-base staining indication reagent, and the acid-base staining indication reagent is eosin, methylene blue, methyl green, trypan blue and Victoria blue B.
In the method and the system for detecting the components of the excrement, a liquid coloring agent is added into the excrement suspension, and the excrement suspension is diluted and colored; adding the fecal suspension after dyeing into a bearing chip; waiting for precipitation and layering of the fecal suspension in the bearing chip; layering and photographing the fecal suspension after the precipitation layering to obtain an image; and analyzing and identifying the image to find out the object to be detected. The turbidimetric dilution card comprises turbidity levels of the suspension liquid and images corresponding to each turbidity level; dilution loading corresponding to each turbidity grade. Diluting and staining the fecal suspension by the reference turbidity card; the turbidimetric card comprises a turbidity grade and a liquid stain loading amount corresponding to the turbidity grade. The liquid state coloring agent can complete dilution and dyeing at the same time, and improves the sample treatment efficiency. The reference turbidity card is used for dilution, so that the dilution consistency of the sample is improved. The top layer image and the bottom layer image can be simultaneously or respectively analyzed to obtain images with different characteristic tangible components, and the analysis efficiency is improved.
While the utility model has been illustrated and described in terms of a preferred embodiment and several alternatives, the application is not limited by the specific description in this specification. Other alternative or equivalent components may also be used in the practice of the present utility model.
Claims (6)
1. A turbidimetric dilution card is characterized in that,
the method comprises turbidity levels of the suspension, and images corresponding to each turbidity level;
also comprises any one or more of the following technical characteristics:
the sample dilution ratio corresponding to the turbidity grade is also included; adding corresponding diluent into the fecal suspension according to the sample dilution ratio;
the method also comprises the sample adding amount of the diluent corresponding to the turbidity grade; adding corresponding diluent to the fecal suspension with the set volume according to the sample adding amount of the diluent;
the sample addition amount corresponding to the turbidity grade is also included; and adding the corresponding fecal suspension into the diluent with the set volume according to the sample addition amount.
2. The turbidimetric dilution card of claim 1, wherein the card is further characterized by,
the sample adding amount of the diluent is increased according to the turbidity grade, and the sample adding amount of the diluent is increased by a set value of the liquid adding amount every time one turbidity grade is increased.
3. The turbidimetric dilution card of claim 1, wherein the image corresponding to each turbidity level comprises a grayscale image or a color image.
4. The turbidimetric dilution card of claim 1, wherein the card is further characterized by,
the dilution liquid sample adding amount is increased according to the turbidity grade, and the dilution liquid sample adding amount is increased by one order of magnitude when one turbidity grade is increased.
5. The turbidimetric dilution card of claim 1, comprising a colorimetric area above the image, the colorimetric area being white.
6. The card of claim 1, wherein the suspension comprises any one of blood, urine, fecal suspension, and soil suspension.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321139251.9U CN220104862U (en) | 2023-05-12 | 2023-05-12 | Turbidimetry dilution card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321139251.9U CN220104862U (en) | 2023-05-12 | 2023-05-12 | Turbidimetry dilution card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220104862U true CN220104862U (en) | 2023-11-28 |
Family
ID=88848055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321139251.9U Active CN220104862U (en) | 2023-05-12 | 2023-05-12 | Turbidimetry dilution card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220104862U (en) |
-
2023
- 2023-05-12 CN CN202321139251.9U patent/CN220104862U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4911172B2 (en) | Analytical apparatus and use thereof | |
| DE60226043T2 (en) | METHOD FOR QUANTITATIVE VIDEO MICROSCOPY AND DEVICE AND PROGRAM FOR IMPLEMENTING THE PROCESS | |
| CN101176116B (en) | Methods of chromogen separation-based image analysis | |
| US6453060B1 (en) | Method and apparatus for deriving separate images from multiple chromogens in a branched image analysis system | |
| JP4085297B2 (en) | Urine component classification device | |
| US20120236120A1 (en) | Automatic stereological analysis of biological tissue including section thickness determination | |
| CN106210520B (en) | A kind of automatic focusing electronic eyepiece and system | |
| CN103534628A (en) | Fast auto-focus in microscopic imaging | |
| CN101339185A (en) | Automatic microscopic imager for detecting cast-off cells and detection method | |
| CA2904401A1 (en) | Imaging blood cells | |
| US20100182417A1 (en) | Automatic focusing apparatus, microscope, and automatic focusing method | |
| JP3924870B2 (en) | Formed component analyzing apparatus and formed component analyzing method | |
| JPH06138120A (en) | Urine-sediment inspecting apparatus | |
| CN220104862U (en) | Turbidimetry dilution card | |
| CN108845406A (en) | The full-automatic micro imaging method of more multiplying powers and device | |
| CN116046647B (en) | Blood imaging analysis system and method | |
| JP4085338B1 (en) | Image processing method | |
| CN111693353A (en) | Detection system integrated with dyeing function and used for microscopic examination | |
| KR102619093B1 (en) | Blood diagnostic apparatus | |
| Peterson | The use of fluorescent probes in cell counting procedures | |
| Allen et al. | Machine vision for automated optical recognition and classification of pollen grains or other singulated microscopic objects | |
| WO2023031622A1 (en) | System and method for identifying and counting biological species | |
| CN114778419A (en) | High-magnification optical magnification imaging flow cytometer | |
| CN114518362A (en) | Sperm quality analysis device, system, method and readable storage medium | |
| CN115901749A (en) | Urine sample detection device and urine sample detection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |