CN220099009U - Rapid monitoring device for ballast water - Google Patents

Abstract

The utility model provides a rapid ballast water monitoring device, which relates to the technical field of ballast water monitoring and has the advantages of providing a more reliable, rapid, effective, standardized, portable device and an enriching device for an entrance and exit inspection and quarantine department, providing technical basis and guiding direction for establishment and popularization of a standardized method in the entrance and exit inspection and quarantine industry.

Description

Rapid monitoring device for ballast water

Technical Field

The utility model relates to the technical field of ballast water monitoring, in particular to a device for rapidly monitoring ballast water.

Background

Along with the global ecological diversity detection and evaluation and ecological health diagnosis requirements, the prevention and control of biological invasion, the protection of endangered species, the evaluation of biological diversity and the evaluation of biomass become common environmental protection behaviors under aquatic ecology.

The ballast water of the present transit port health epidemic department is responsible for hazard monitoring of the ballast water of the international navigation ship, wherein the ballast water of the cholera epidemic area, the ballast water filled in other epidemic areas but discharged in the berthing port, the imported old steel vessel, the maintenance vessel, the water required to be monitored by national regulations and the like need to be subjected to health monitoring, and the urban inspection and quarantine standards (SN/1875-2007) of the people's republic of China mainly indicate that the urban ship is under the important project of the national export and entry water for the national inspection and quarantine industry, including salmonella, shibrio, salmonella, hepatitis A, vibrio and the like.

The method for monitoring different microorganisms is also specified in the standard (see the detection method in the detection procedure for details), and the operation comprises the steps of sampling quantitative ballast water to enrich the quantitative ballast water through a natural shunt water pipe to a 50um plankton collection net, and finally back flushing residual substances in the collection net and carrying back flushing liquid to a laboratory for subsequent re-enrichment, cracking, purification, amplification, electrophoresis and gel imaging treatment to judge results, however, the existing ballast water monitoring method has the problems that the sampling process is difficult to standardize, the enrichment speed is low, the sampling equipment is simple, environmental samples are easy to pollute, the water sample is inconvenient to carry, the work repeatability is high, and the monitored objects are incomplete.

Disclosure of Invention

In order to overcome the defects in the prior art, the utility model provides a rapid monitoring device for ballast water, which comprises a box body, a connecting flange arranged outside the box body and an enriching device which is positioned outside the box body and is used for enriching DNA related substances in the ballast water, wherein the inner cavity of the box body is hollow, a positive pressure pump is fixed in the inner cavity of the box body, a water delivery port and a water discharge port are arranged on the box body, the connecting flange is communicated with the water delivery port of the box body through a connecting pipe, a water inlet of the positive pressure pump is communicated with the water delivery port of the box body through a pipeline, a water outlet of the positive pressure pump is communicated with the water inlet of the enriching device through a pipeline, and a water outlet of the enriching device is communicated with the water discharge port of the box body through a pipeline.

In order to achieve the above purpose, through the effect of the connecting flange, so that the ballast water drain pipe is communicated with the connecting pipe, through the effect of the positive pressure pump, the ballast water enters the water delivery port of the box body through the connecting pipe, the ballast water entering the water delivery port of the box body enters the enriching device, the environment DNA related substances in the ballast water are enriched through the effect of the enriching device, the rest of the ballast water is discharged through the water outlet of the box body, the enriching device is taken down and the sampled enriching device is sent to a laboratory, based on the mature equipment and process of the environment DNA technology, the specific species monitoring in the water body is accurately and rapidly judged through the elution, DNA extraction, purification, fragment amplification and QPCR technology, so as to achieve the analysis, monitoring and management of the water environment condition of the ballast water, the early warning and monitoring of the species under the water environment, the data support are provided, the technical support is provided for the establishment of the monitoring method standardization, the management and protection establishment of the monitoring method of the species in the detection department, the rapid monitoring of the species in the detection department is realized, the environmental protection policy is provided for the inspection and the environmental protection department, the environmental protection system is provided with a more reliable, the rapid and effective, the sample collection method is simple, the sample collection and the environmental protection system is carried, the environmental protection object is not convenient, and the environmental protection object is not convenient.

Further, a V1 electromagnetic valve is arranged on a pipeline between the positive pressure pump and the enrichment device, a water outlet of the positive pressure pump is communicated with a water inlet of the V1 electromagnetic valve through a pipeline, a water outlet of the V1 electromagnetic valve is communicated with a water inlet of the enrichment device through a pipeline, and a pressure regulating pipeline is arranged on the pipeline between the positive pressure pump and the V1 electromagnetic valve.

Through the technical scheme, the on-off of a pipeline between the positive pressure pump and the enrichment device is controlled through the action of the V1 electromagnetic valve, and the flow of the pipeline is controlled through the pressure regulating pipeline, so that the adjustment of the flow and the pressure of ballast water entering the enrichment device is achieved.

Further, the pressure regulating pipeline comprises a pressure regulating valve, a pipeline between the positive pressure pump and the V1 electromagnetic valve is communicated with a water inlet of the pressure regulating valve through a pipeline, and a water outlet of the pressure regulating valve is communicated with a water outlet of the box body through a pipeline.

Through the technical scheme, part of ballast water entering the positive pressure pump enters the enrichment device through the V1 electromagnetic valve, and the other part of ballast water enters the pressure regulating valve, wherein the pressure regulating valve is a valve for controlling the flow of a pipeline and guiding, and the diversion in the pipeline is controlled through manual opening, closing or adjusting, so that the adjustment of the flow and the pressure of the ballast water entering the enrichment device is achieved, and the effect of protecting a pipeline is achieved.

Further, an air inlet is arranged on the box body, and an emptying pipeline which is matched with the air inlet and is used for completely discharging the residual ballast water in the enrichment device is arranged on the box body.

Through the technical scheme, when the flow rate of the ballast water passing through the enrichment device reaches a set value, the positive pressure pump and the V1 electromagnetic valve are closed, and the residual ballast water in the enrichment device is completely discharged through the action of the emptying pipeline, so that the aim of emptying the enrichment device is fulfilled.

Further, the emptying pipeline comprises a V2 electromagnetic valve, an input port of the V2 electromagnetic valve is communicated with an air inlet of the box body through a pipeline, an output port of the V2 electromagnetic valve is communicated with a pipeline between the V1 electromagnetic valve and the enriching device, a negative pressure pump is arranged on the pipeline between the enriching device and a water outlet of the box body, a water outlet of the enriching device is communicated with a water inlet of the negative pressure pump through a pipeline, and a water outlet of the negative pressure pump is communicated with a water inlet of the box body through a pipeline.

Through the technical scheme, when the water quantity to be enriched reaches the set value, the system controls to close the positive pressure pump, the V1 electromagnetic valve, open the V2 electromagnetic valve and the negative pressure pump, and through the action of the negative pressure pump, the air inlet sucks air to discharge residual ballast water in the enrichment device from the water outlet of the box body through the negative pressure pump, so that the purpose of emptying the enrichment device is achieved.

Further, the enrichment ware includes the shell, the shell is cavity, upper and lower both ends open-ended tubular structure, the inside A filter core that is provided with of enrichment ware, A filter core is cavity, upper and lower both ends open-ended tubular structure, the external diameter of A filter core is less than the internal diameter of shell, the central line of shell and the coincidence of the central line of A filter core, the top of shell is fixed with the upper cover that seals shell open-top portion and A filter core open-top portion, be provided with the top connection that communicates and be convenient for intaking with the shell cavity on the upper cover, shell bottom is fixed with the lower cover that seals shell bottom open-top portion and A filter core open-top portion, be provided with the lower connection that communicates and be convenient for drain with A filter core cavity down on the lower cover.

Through above-mentioned technical scheme, in the ballast water gets into shell, upper cover, lower cover and the cavity that forms outside the A filter core through the upper connector, later gets into A filter core inner chamber after filtering through A filter core, and discharges through the lower clutch with A filter core inner chamber intercommunication, through the cooperation between shell, upper cover, lower cover and the A filter core, the ballast water that gets into the shell inner chamber through the upper connector can not directly get into A filter core inner chamber and discharge through the lower clutch without filtering through A filter core.

Further, O-shaped rings for improving sealing performance are arranged at the joint of the upper cover and the shell, the joint of the upper cover and the A filter element, the joint of the lower cover and the shell and the joint of the lower cover and the A filter element.

Through above-mentioned technical scheme, through the effect of O type circle, improved upper cover and shell junction, upper cover and A filter core junction, lower cover and shell junction and lower cover and A filter core junction's leakproofness, reduce the possibility that the ballast water revealed.

Further, the enrichment device comprises an outer cover, the inner cavity of the outer cover is hollow, an upper end pipe communicated with the inner cavity of the outer cover is arranged at the middle position of the top end of the outer cover, a B filter element is fixed at the middle position of the bottom of the inner cavity of the outer cover, the center line of the B filter element is coincident with the center line of the outer shell, the outer diameter of the B filter element is smaller than the diameter of the inner cavity of the outer cover, the inner cavity of the B filter element is hollow, and a lower end pipe communicated with the inner cavity of the B filter element is fixed at the middle position of the bottom end of the outer cover.

Through the technical scheme, the cavity formed by the upper end pipe and the inner cavity of the outer cover and the cavity formed by the inner cavity of the B filter element and the lower end pipe are two cavities which are not communicated with each other, so that the ballast water enters the inner cavity of the outer cover through the upper end pipe, the ballast water only enters the inner cavity of the B filter element through the B filter element and cannot directly enter the lower end pipe through the action of the B filter element, the environment DNA related substances in the ballast water are enriched in the enriching device, and the rest of the ballast water is discharged through the lower end pipe communicated with the inner cavity of the B filter element.

In summary, the rapid ballast water monitoring device has the following beneficial effects:

(1) The rapid monitoring device for ballast water is characterized in that a connecting flange is used for facilitating the communication of a ballast water drain pipe and a connecting pipe, ballast water enters a water delivery port of a box body through the connecting pipe under the action of a positive pressure pump, the ballast water entering the water delivery port of the box body enters an enrichment device, the environment DNA related substances in the ballast water are enriched under the action of the enrichment device, the rest of the ballast water is discharged through a water outlet of the box body, the enrichment device is taken down, the sampled enrichment device is sent to a laboratory, the equipment and the process are mature based on the environment DNA technology, and the targeted species monitoring in the water body is accurately and rapidly judged through an elution, DNA extraction, purification, fragment amplification and QPCR technology real-time fluorescence quantitative nucleic acid amplification detection system, the method is used for analyzing, monitoring and managing the water environment condition of the ballast water, providing data support for early warning and monitoring of invasive species in the water environment, providing technical support for standardization, management and establishment of protection policy establishment of monitoring methods for ballast water of detection departments, realizing rapid monitoring of the invasive species in the ballast water, providing a more reliable, rapid, effective and standardized monitoring method for entry and exit inspection and quarantine departments, and solving the problems of low enrichment speed, simple sampling equipment, easy pollution of environmental samples, inconvenient water sample carrying, work repeatability and incomplete monitoring objects.

(2) This to quick monitoring devices of ballast water, through the effect of negative pressure pump, the air inlet is inhaled, discharges the interior residual ballast water of enrichment ware from the box outlet through the negative pressure pump, adopts positive pressure and negative pressure to combine together the mode, reaches quick and speed adjustable enrichment sample purpose, improves enrichment efficiency and satisfies multiple sample enrichment operating mode demand, reaches the enrichment of total environment DNA maximum in the ration time. Saving manpower, material resources and time cost.

(3) This to quick monitoring devices of ballast water, through pressure adjusting pipeline, according to the sampling environment at any time adjustment sample enrichment speed, satisfy the environment full coverage of sample sampling.

(4) The pressure-bearing sealing and tubular filter element structure is adopted in the enriching device for the ballast water rapid monitoring device, the volume is small, the filtering area is large, and the requirements of high-capacity and high-pressure rapid enrichment are met; the method is more effective in evaluating special species (such as endangered species and the like), is suitable for the full-coverage enrichment environment of the more complex water ecology eDNA sample, and is easy to popularize in a standardized way in a sampling process and an enriching device.

(5) The rapid monitoring device for the ballast water adopts enrichment, emptying and other processes and a rapid disassembly and assembly structure of an enrichment device in the sampling process, and is easy to meet the standardization of the sampling process, and the enrichment device is modularized.

Drawings

The utility model is further described and illustrated below with reference to the accompanying drawings.

FIG. 1 is a schematic overall construction of a preferred embodiment of the present utility model;

FIG. 2 is a schematic diagram of a structure for embodying a code scanning window of the present utility model;

fig. 3 is a schematic cross-sectional structure of a case of embodiment 1 of the present utility model;

FIG. 4 is a schematic diagram of the structure of the present utility model for embodying an A cartridge;

FIG. 5 is a control circuit schematic of embodiment 1 of the present utility model;

fig. 6 is a schematic sectional structure of a case of embodiment 2 of the present utility model;

FIG. 7 is a schematic diagram of a structure embodying a B-cartridge of the present utility model;

fig. 8 is a piping diagram of embodiment 2 of the present utility model.

Reference numerals: 1. a case; 101. a water delivery port; 102. a water outlet; 103. an air inlet; 104. a positive pressure pump; 105. v1 electromagnetic valve; 106. a pressure regulating valve; 107. v2 electromagnetic valve; 108. a negative pressure pump; 109. a pressure sensor; 110. v3 electromagnetic valve; 111. f1 flowmeter; 112. a pressure gauge; 113. f2 flow meter; 2. a connecting flange; 3. a connecting pipe; 4. an enriching device; 401. a housing; 402. a, a filter element; 403. an upper cover; 404. an upper joint; 405. a lower cover; 406. a lower joint; 407. an O-ring; 408. an outer cover; 409. an upper end tube; 410. a filter element B; 411. a lower end tube; 5. a circuit board; 6. a human-machine interface; 7. a code scanning window; 8. and a pressure release valve.

Detailed Description

The technical solution of the present utility model will be more clearly and completely explained by the description of the preferred embodiments of the present utility model with reference to the accompanying drawings.

Example 1: referring to fig. 1-5, in the embodiment of the utility model, a rapid monitoring device for ballast water comprises a box body 1, a connecting flange 2 which is positioned outside the box body 1 and connected with a ballast water drain pipe pipeline, and an enriching device 4 which is positioned outside the box body 1 and used for enriching environment DNA related substances in the ballast water, wherein the inner cavity of the box body 1 is hollow, a positive pressure pump 104 is fixed in the inner cavity of the box body 1, a water inlet 101 and a water outlet 102 are arranged on the box body 1, one end of the connecting flange 2, which is far away from the ballast water drain pipe, is communicated with the water inlet 101 of the box body 1 through a connecting pipe 3, a water inlet of the positive pressure pump 104 is communicated with the water inlet 101 of the box body 1 through a pipeline, and a water outlet of the enriching device 4 is communicated with the water outlet 102 of the box body 1 through a pipeline.

As shown in fig. 1 and 2 and 3, through the function of the connecting flange 2 so that the ballast water drain pipe is communicated with the connecting pipe 3, through the function of the positive pressure pump 104, the ballast water enters the water delivery port 101 of the box body 1 through the connecting pipe 3, the ballast water entering the water delivery port 101 of the box body 1 enters the enriching device 4, the environment DNA related substances in the ballast water are enriched through the function of the enriching device 4, the rest ballast water is discharged through the water outlet 102 of the box body 1, the enriching device 4 is removed, the sampled enriching device 4 is sent to a laboratory, the specific species monitoring in the water body is accurately and rapidly judged through the real-time fluorescence quantitative nucleic acid amplification detection system of the elution, DNA extraction, purification, fragment amplification and QPCR technology based on the mature equipment and technology of the environment DNA technology, the method is used for analyzing, monitoring and managing the water environment condition of the ballast water, providing data support for early warning and monitoring of invasive species in the water environment, providing technical support for standardization, management and establishment of protection policy establishment of monitoring methods for ballast water of detection departments, realizing rapid monitoring of the invasive species in the ballast water, providing a more reliable, rapid, effective and standardized monitoring method for entry and exit inspection and quarantine departments, and solving the problems of low enrichment speed, simple sampling equipment, easy pollution of environmental samples, inconvenient water sample carrying, work repeatability and incomplete monitoring objects.

As shown in fig. 3 and 5, a V1 electromagnetic valve 105 is arranged on a pipeline between the positive pressure pump 104 and the enriching device 4, a water outlet of the positive pressure pump 104 is communicated with a water inlet of the V1 electromagnetic valve 105 through a pipeline, a water outlet of the V1 electromagnetic valve 105 is communicated with a water inlet of the enriching device 4 through a pipeline, and the on-off of the pipeline between the positive pressure pump 104 and the enriching device 4 is controlled through the action of the V1 electromagnetic valve 105.

In order to adjust the flow and pressure of ballast water into the concentrator 4, as shown in fig. 3 and 5, a pressure adjusting line is provided between the positive pressure pump 104 and the V1 solenoid valve 105.

As shown in fig. 3 and 5, the pressure regulating pipeline comprises a pressure regulating valve 106, a pipeline between a positive pressure pump 104 and a V1 electromagnetic valve 105 is communicated with a water inlet of the pressure regulating valve 106 through a pipeline, a water outlet of the pressure regulating valve 106 is communicated with a water outlet 102 of the box body 1 through a pipeline, ballast water entering the positive pressure pump 104 enters the enrichment device 4 through the V1 electromagnetic valve 105, and the other part enters the pressure regulating valve 106, the pressure regulating valve 106 is a valve for controlling pipeline flow and guiding, and the diversion in the pipeline is controlled through manual opening, closing or adjusting, so that the regulation of the ballast water flow and pressure entering the enrichment device 4 is achieved, and the effect of protecting the pipeline is achieved.

As shown in fig. 3 and 5, a pressure sensor 109 is arranged on the pipeline between the positive pressure pump 104 and the pressure regulating valve 106, and the pressure of water flowing through the pressure regulator is read through the action of the pressure sensor 109.

As shown in fig. 5, when the flow rate of the ballast water passing through the enriching machine 4 reaches a set value, the positive pressure pump 104 and the V1 solenoid valve 105 are closed, and at this time, the residual ballast water in the enriching machine 4 is difficult to discharge, so that the tank 1 is provided with a discharge line for discharging the whole ballast water remaining in the enriching machine 4.

As shown in fig. 3 and 5, the tank 1 is further provided with an air inlet 103, the emptying pipeline comprises a V2 electromagnetic valve 107, an input port of the V2 electromagnetic valve 107 is communicated with the air inlet 103 of the tank 1 through a pipeline, an output port of the V2 electromagnetic valve 107 is communicated with a pipeline between the V1 electromagnetic valve 105 and the enrichment device 4, a negative pressure pump 108 is arranged on a pipeline between the enrichment device 4 and a water outlet 102 of the tank 1, a water outlet of the enrichment device 4 is communicated with a water inlet of the negative pressure pump 108 through a pipeline, a water outlet of the negative pressure pump 108 is communicated with a water inlet of the tank 1 through a pipeline, when the water to be enriched reaches a set value, the system controls to close the positive pressure pump 104 and the V1 electromagnetic valve 105, opens the V2 electromagnetic valve 107 and the negative pressure pump 108, and through the action of the negative pressure pump 108, the air inlet 103 sucks air, and residual ballast water in the enrichment device 4 is discharged from the water outlet 102 of the tank 1 through the negative pressure pump 108, so as to achieve the aim of emptying the enrichment device 4.

As shown in fig. 3 and 5, a V3 electromagnetic valve 110 is arranged on a pipeline between the enriching device 4 and the negative pressure pump 108, a water outlet of the enriching device 4 is communicated with a water inlet of the V3 electromagnetic valve 110 through a pipeline, a water outlet of the V3 electromagnetic valve 110 is communicated with a water inlet of the negative pressure pump 108 through a pipeline, and the opening and closing of the pipeline between the enriching device 4 and the negative pressure pump 108 are controlled through the action of the V3 electromagnetic valve 110.

As shown in fig. 3 and 5, a F1 flowmeter 111 is arranged in a pipeline between the negative pressure pump 108 and the water outlet 102 of the tank 1, the water outlet of the negative pressure pump 108 is communicated with the water inlet of the F1 flowmeter 111 through a pipeline, the water outlet of the F1 flowmeter 111 is communicated with the water outlet 102 of the tank 1 through a pipeline, and the flow rate of the ballast water in the enrichment device 4 discharged out of the water outlet 102 is read through the action of the F1 flowmeter 111.

As shown in fig. 3 and 5, check valves for controlling the opening and closing of the pipelines are arranged on the pipeline between the water delivery port 101 and the positive pressure pump 104 of the box body 1, the pipeline between the positive pressure pump 104 and the V1 electromagnetic valve 105, the pipeline between the V3 electromagnetic valve 110 and the negative pressure pump 108, and the pipeline between the negative pressure pump 108 and the water outlet 102 of the box body 1.

As shown in fig. 4, the enriching device 4 comprises a housing 401, the housing 401 is a tubular structure with a hollow inner cavity and open upper and lower ends, an a filter element 402 is arranged in the enriching device 4, the a filter element 402 is a tubular structure with a hollow inner cavity and open upper and lower ends, the outer diameter of the a filter element 402 is smaller than the inner diameter of the housing 401, the central line of the housing 401 coincides with that of the a filter element 402, an upper cover 403 for closing the top opening part of the housing 401 and the top opening part of the a filter element 402 is fixed on the top end of the housing 401, an upper joint 404 which is communicated with the inner cavity of the housing 401 and is convenient for water inflow is arranged on the upper cover 403, a lower cover 405 for closing the bottom opening part of the housing 401 and the bottom opening part of the a filter element 402 is fixed on the bottom end of the housing 401, and a lower joint 406 which is communicated with the inner cavity of the a filter element 402 and is convenient for water drainage is arranged on the lower cover 405.

As shown in fig. 4, the connection between the upper cover 403 and the housing 401, the connection between the upper cover 403 and the a filter element 402, the connection between the lower cover 405 and the housing 401, and the connection between the lower cover 405 and the a filter element 402 are all provided with O-rings 407 for improving the tightness, and the housing 401, the upper cover 403, the a filter element 402, and the lower cover 405 form two cavities which are not communicated with each other between the inner cavity of the housing 401 and the inner cavity of the filter element through the O-rings 407.

As shown in fig. 4, ballast water enters the cavity formed outside the housing 401, the upper cover 403, the lower cover 405 and the a filter cartridge 402 through the upper connector 404, then enters the cavity of the a filter cartridge 402 after being filtered by the a filter cartridge 402, and is discharged through the lower connector 406 communicated with the cavity of the a filter cartridge 402, and through the cooperation among the housing 401, the upper cover 403, the lower cover 405 and the a filter cartridge 402, the ballast water entering the cavity of the housing 401 through the upper connector 404 cannot directly enter the cavity of the a filter cartridge 402 without being filtered by the a filter cartridge 402 and is discharged through the lower connector 406.

As shown in fig. 4, the housing 401, the upper cover 403, the lower cover 405 and the a filter element 402 may be split or integrated, and the a filter element 402 adopts a microporous filter membrane to filter 2-5um, and has the characteristics of small volume, large filtering area, large sewage containing amount, corrosion resistance, high pressure resistance, low cost, heat resistance, easy sterilization and easy elution, and the a filter element 402 has a tubular or umbrella-shaped structure and may be made of polyester fiber, polyethersulfone or other materials.

As shown in fig. 2, the pipeline between the V1 electromagnetic valve 105 and the enriching device 4 is threaded through the box 1, the upper joint 404 is connected with the part of the pipeline between the V1 electromagnetic valve 105 and the enriching device 4, which is threaded through the box 1, the pipeline between the water inlet of the V3 electromagnetic valve 110 and the enriching device 4 is threaded through the box 1, the lower joint 406 is connected with the part of the pipeline between the water inlet of the V3 electromagnetic valve 110 and the enriching device 4, which is threaded through the box 1, and after the sampling is finished, the upper joint 404 and the lower joint 406 are detached through the quick plug and the pipeline, so that the enriching device 4 is conveniently disconnected with the box 1, and the sampled enriching device 4 is conveniently sent to a laboratory.

As shown in fig. 3, a circuit board 5 is further fixed in the box 1, a control module, a power supply module for supplying power to the device, a wireless module and a code scanning module are arranged on the circuit board 5, the control module is an autonomous programming singlechip, the power supply module can be a battery, a solar panel or commercial power, the power supply module is connected with the circuit board 5 through wires, a man-machine interface 6 and a code scanning window 7 are arranged on the outer wall of the box 1, an identification code is arranged on the outer wall of the enrichment device 4, a V1 electromagnetic valve 105, a V2 electromagnetic valve 107, a V3 electromagnetic valve 110, a positive pressure pump 104, a negative pressure pump 108, a pressure sensor 109, a F1 flowmeter 111, a code scanning window 7, a code scanning module and the man-machine interface 6 are connected with the circuit board 5 through wires, and the V1 electromagnetic valve 105, the V2 electromagnetic valve 107, the V3 electromagnetic valve 110, the positive pressure pump 104, the negative pressure pump 108, the pressure sensor 109, the F1 flowmeter 111 and the code scanning window 7 are electrically connected with the control module.

As shown in fig. 3, the data collected by the pressure sensor 109 and the F1 flowmeter 111 are transmitted to the control module, and the data collected by the pressure sensor 109 and the F1 flowmeter 111 are displayed on the man-machine interface 6 in a visual manner under the action of the control module, and a timer and a memory are further arranged on the control module.

As shown in fig. 3, the system further comprises an intelligent display terminal and a server, the circuit board 5 is further provided with a GPS and a wireless module, the control module is electrically connected with the GPS, and the GPS is used for transmitting data such as longitude, latitude, altitude and the like of the current GPS to a designated server through a GPRS or 3G network, and the server transmits the data to the intelligent display terminal to map the position and track identification on the software.

When the device is used, firstly, the standardized enrichment device 4 is subjected to high-temperature sterilization treatment, after the device is brought to the site, the identification code of the enrichment device 4 is identified through the code scanning window 7 of the box body 1, then the water inlet of the box body 1 and the ballast water drain pipe are built through the connecting flange 2 and the connecting pipe 3, the upper joint 404 and the lower joint 406 of the enrichment device 4 are respectively connected with the upper pipe joint of the box body 1 in a quick-plugging manner, the device is electrified, ballast water is sucked into the pipe through the connecting pipe 3 by the positive pressure pump 104, the positive pressure pump 104 is a self-priming pump or a submersible pump, and a part of water sequentially enters the V1 electromagnetic valve 105, the enrichment device 4, the V3 electromagnetic valve 110, the negative pressure pump 108 and the F1 flowmeter 111 and is discharged through the water outlet 102 of the box body 1;

the other part of the ballast water sequentially passes through the pressure sensor 109 and the pressure regulating valve 106, is discharged through the water outlet 102 of the box body 1, detects the pressure of the ballast water in the pipeline through the action of the pressure sensor 109, controls the diversion in the pipeline through the action of the pressure regulating valve 106, adjusts the internal water flow, adjusts the ballast water flow entering the enrichment device 4 under the condition that the water delivery port 101 flows at a certain rate, and then adjusts the pressure passing through the filter element 402 of the enrichment device 4A, thereby achieving the purpose of adjusting the enrichment speed;

when the ballast water flows through the A filter element 402, the environment DNA related substances in the ballast water are adsorbed on the surface of the A filter element 402, so that the purpose of enrichment is achieved;

setting a ballast water flow set value flowing through the enriching device 4 through the action of a control module, closing a positive pressure pump 104 and a V1 electromagnetic valve 105 when the ballast water flow passing through the enriching device 4 reaches the set value, opening a V2 electromagnetic valve 107 and a negative pressure pump 108, wherein the negative pressure pump 108 is a negative pressure vacuum pump, sucking air through an air inlet 103 through the action of the negative pressure pump 108, and discharging residual ballast water in the enriching device 4 from a water outlet 102 of a box body 1 through a V3 electromagnetic valve 110, the negative pressure pump 108 and an F1 flowmeter 111, so that the aim of emptying the enriching device 4 is achieved, the sampling and enriching process is completed, the intelligent degree is high, the sampling efficiency is high, and time and labor are saved;

then the enriching device 4 is taken down, the sampled enriching device 4 is sent to a laboratory, and the targeted species monitoring in the water body is accurately and rapidly judged by eluting, extracting, purifying, amplifying fragments and detecting the real-time fluorescence quantitative nucleic acid amplification system by the QPCR technology based on the mature equipment and process of the environmental DNA technology;

meanwhile, the device control module combines the identification code, the sampling time and the location coordinates of the enrichment device 4, and realizes the track marking of the sampling position of the sample in the map software through a GPS, a remote control terminal and the like, thereby achieving the position and track positioning identification of the sample of the enrichment device 4.

Example 2: a rapid monitoring device for ballast water based on fig. 1, which differs from example 1 in that:

referring to fig. 6-8, the emptying pipeline further comprises a pressure gauge 112 and a F2 flowmeter 113, the pressure gauge 112 and the F2 flowmeter 113 are arranged on the pipeline between the V1 electromagnetic valve 105 and the enrichment device 4, the water outlet of the V1 electromagnetic valve 105 is communicated with the water inlet of the pressure gauge 112 through the pipeline, the water outlet of the pressure gauge 112 is communicated with the water inlet of the F2 flowmeter 113 through the pipeline, the water outlet of the F2 flowmeter 113 is communicated with the water inlet of the enrichment device 4 through the pipeline, and the pressure and the flow of the ballast water flowing into the enrichment device 4 are monitored through the functions of the pressure gauge 112 and the F2 flowmeter 113.

As shown in fig. 6 and 8, a pressure release valve 8 is further arranged on the pipeline between the positive pressure pump 104 and the V1 electromagnetic valve 105, the pipeline between the positive pressure pump 104 and the V1 electromagnetic valve 105 is communicated with the water inlet of the pressure release valve 8 through the pipeline, the water outlet of the pressure release valve 8 is communicated with the water outlet 102 of the box body 1 through the pipeline, when the pressure of the pipeline flowing through the enriching device 4 reaches the threshold value of the pressure release valve 8 based on the protection of the pipeline system and the blocking condition of the enriching device 4, the pressure release valve 8 is automatically opened for pressure release, and the water path is discharged from the water outlet 102 through the pressure release valve 8, so that the pipeline purpose of the protecting device is achieved.

As shown in fig. 6 and 8, check valves are provided in the line between the positive pressure pump 104 and the pressure regulating valve 106, in the line between the concentrator 4 and the drain port 102 of the tank 1, and in the line between the V2 solenoid valve 107 and the F2 flowmeter 113.

As shown in fig. 6 and 8, the water inlet of the negative pressure pump 108 is connected in parallel with the pipeline between the water outlet of the enriching device 4 and the water inlet of the one-way valve to form a sealing pipeline, and the water outlet of the negative pressure pump 108 is connected in parallel with the pipeline between the water outlet of the one-way valve and the water outlet 102 of the box body 1 to form a sealing pipeline.

Referring to fig. 7, the concentrator 4 includes a housing 408, the housing 408 has a tubular structure with a hollow inner cavity and open upper and lower ends, a B filter element 410 is fixed in the inner cavity of the housing 408, the B filter element 410 is preferably a cylindrical or umbrella-shaped microporous filter membrane structure made of 0.45 μm PES material, the B filter element 410 has a hollow inner cavity, a top is closed, a bottom is open and a barrel-shaped structure, the center line of the B filter element 410 coincides with the center line of the inner cavity of the housing 408, an upper end tube 409 communicated with the inner cavity of the housing 408 is fixed on the upper end surface of the housing 408, and a lower end tube 411 communicated with the inner cavity of the B filter element 410 is fixed on the lower surface of the housing 408.

As shown in FIG. 7, the inner cavity of the outer cover 408 and the inner cavity of the B filter element 410 form two cavities which are not communicated with each other, so that under the action of the B filter element 410, the ballast water can only enter the inner cavity of the B filter element 410 through the B filter element 410, the environment DNA related substances in the ballast water are enriched in the enriching device 4, and the rest of the ballast water is discharged through the lower end pipe 411 communicated with the inner cavity of the filter element.

As shown in fig. 6 and 7, the upper pipe 409 is connected with the water outlet of the F2 flowmeter 113 through a quick connector, and the lower pipe 411 is connected with the check valve through a quick connector, so as to facilitate the installation and the disassembly of the enrichment device 4.

As shown in fig. 6, the F2 flowmeter 113 and the pressure gauge 112 are both connected with the circuit board 5 by wires, and the F2 flowmeter 113 and the pressure gauge 112 are electrically connected with the control module.

When the device is used, firstly, the standardized enrichment device 4 is subjected to high-temperature sterilization treatment, after the device is brought to the site, the identification code of the enrichment device 4 is identified through the code scanning window 7 of the box body 1, then the water inlet of the box body 1 and the ballast water drain pipe are built through the connecting flange 2 and the connecting pipe 3, the upper end pipe 409 and the lower end pipe 411 of the enrichment device 4 are respectively and quickly connected with the pipeline interface on the box body 1, the device is electrified, ballast water is sucked into the pipeline through the connecting pipe 3 through the positive pressure pump 104, and a part of water sequentially enters the V1 electromagnetic valve 105, the pressure gauge 112, the F2 flowmeter 113 and the enrichment device 4 and is discharged through the water outlet 102 of the box body 1;

the other part of the ballast water enters the pressure regulating valve 106 and is discharged through the water outlet 102 of the box body 1, the diversion in the pipeline is controlled through the action of the pressure regulating valve 106, the internal water flow is regulated, and under the condition that the flow of the water delivery port 101 is constant, the ballast water flow entering the enrichment device 4 is regulated, so that the pressure passing through the filter element 410 of the enrichment device 4B is regulated, and the purpose of regulating the enrichment speed is achieved;

when the ballast water flows through the B filter element 410, the environment DNA related substances in the ballast water are adsorbed on the surface of the B filter element 410, so that the purpose of enrichment is achieved;

the set value of the ballast water flow flowing through the enriching device 4 is set through the action of the control module, when the ballast water flow passing through the enriching device 4 reaches the set value, the positive pressure pump 104 and the V1 electromagnetic valve 105 are closed, the V2 electromagnetic valve 107 and the negative pressure pump 108 are opened, the air inlet 103 sucks air through the action of the negative pressure pump 108, and the residual ballast water in the enriching device 4 is discharged from the water outlet 102 of the box body 1, so that the aim of emptying the enriching device 4 is fulfilled, the sampling and enriching process is finished, the intelligent degree is high, the sampling efficiency is high, and the time and the labor are saved;

and then the enriching device 4 is taken down, the sampled enriching device 4 is sent to a laboratory, and the targeted species monitoring in the water body is accurately and rapidly judged by eluting, extracting DNA, purifying, amplifying fragments and detecting the real-time fluorescence quantitative nucleic acid amplification system by the QPCR technology based on the mature equipment and process of the environmental DNA technology.

Based on the two devices of the embodiment 1 and the embodiment 2, a method for rapidly monitoring ballast water is provided, which comprises the following specific steps:

step 1: subjecting the standardized concentrator 4 to a high temperature sterilization treatment;

step 2: bringing a standardized enrichment device 4 to the site, constructing a water delivery port 101 of the box body 1 and a ballast water drain pipe through a connecting flange 2 and a connecting pipe 3, and connecting the enrichment device 4 and the box body 1 through quick insertion;

step 3: when the rapid ballast water monitoring device in the embodiment 1 is used, a positive pressure pump 104 is used for sucking ballast water into a pipeline, a part of the ballast water sequentially passes through a V1 electromagnetic valve 105, an enrichment device 4, a V3 electromagnetic valve 110, a negative pressure pump 108 and a flowmeter, then is discharged through a water outlet 102 of a box body 1, and the other part of the ballast water enters a pressure regulating valve 106 through a pressure sensor 109 and is discharged through the water outlet 102 of the box body 1, environment DNA related substances in the ballast water are adsorbed on the surface of an A filter element 402, when the ballast water flow rate passing through the enrichment device 4 reaches a set value, the V1 electromagnetic valve 105 is closed, the V2 electromagnetic valve 107 and the negative pressure pump 108 are opened, an air inlet 103 is sucked, residual ballast water in the enrichment device 4 is discharged from the water outlet 102 of the box body 1 through the V3 electromagnetic valve 110, the negative pressure pump 108 and the F1 flowmeter 111, so that the purpose of emptying the enrichment device 4 is achieved, and the sampling enrichment process is completed;

when the rapid ballast water monitoring device in the embodiment 2 is used, the ballast water is sucked into a pipeline through the positive pressure pump 104, a part of the ballast water sequentially passes through the V1 electromagnetic valve 105, the pressure gauge 112, the F2 flowmeter 113 and the enriching device 4 and is discharged through the water outlet 102 of the box body 1, the environment DNA related substances in the ballast water are adsorbed on the surface of the A filter element 402, the other part of the ballast water enters the pressure regulating valve 106 to regulate the enriching speed, when the pipeline pressure flowing through the enriching device 4 reaches the threshold value of the pressure relief valve 8, the pressure relief valve 8 is automatically opened to relieve pressure, the water channel is discharged through the pressure relief valve 8 to the water outlet 102, the pipeline purpose of the protecting device is further achieved, when the ballast water flow passing through the enriching device 4 reaches the set value, the V1 electromagnetic valve 105 is closed, the V2 electromagnetic valve 107 and the negative pressure pump 108 are opened, the air inlet 103 sucks air, the residual ballast water in the enriching device 4 is discharged from the water outlet 102 of the box body 1 through the negative pressure pump 108, the aim of emptying the enriching device 4 is achieved, and the sampling enriching process is completed.

Step 4: after the device is subjected to water enrichment quantity setting, enrichment and emptying processes are finished, the sampling process is finished, and the enriching device 4 is detached from the box body 1 and sealed for storage; and finally, the connecting flange 2 is disassembled, and the on-site pipeline is restored to complete the whole set of process sampling.

Step 5: taking the sampled filter to a laboratory, and accurately and rapidly judging the monitoring of the targeted species in the water body by using a real-time fluorescent quantitative nucleic acid amplification detection system based on the mature equipment and process of the environmental DNA technology through elution, DNA extraction, purification, fragment amplification and QPCR technology so as to realize the analysis, monitoring and management of the water environment condition of the ballast water; the early warning and monitoring of invasive species in the water environment are achieved, and data support is provided; and provides technical support for standardization, management and protection policy establishment of monitoring methods of the detection department for the ballast water. The method has the characteristics of high sampling, DNA extraction, amplification, QPCR analysis related products, process, high analysis maturity, more accuracy, rapidness and the like.

The above detailed description is merely illustrative of the preferred embodiments of the present utility model and is not intended to limit the scope of the present utility model. Various modifications, substitutions and improvements of the technical scheme of the present utility model will be apparent to those skilled in the art from the description and drawings provided herein without departing from the spirit and scope of the utility model. The scope of the utility model is defined by the claims.

Claims (8)

1. The utility model provides a to quick monitoring devices of ballast water, its characterized in that includes box (1), set up flange (2) outside box (1) and be located box (1) outside and be used for enriching enrichment ware (4) of environment DNA related substance in the ballast water, box (1) inner chamber cavity, be fixed with positive pressure pump (104) in box (1) inner chamber, be equipped with water delivery port (101) and outlet (102) on box (1), flange (2) and water delivery port (101) of box (1) are through connecting pipe (3) intercommunication, water delivery port (101) of positive pressure pump (104) are through the pipeline intercommunication, the delivery port of positive pressure pump (104) and the water inlet of enrichment ware (4) are through the pipeline intercommunication, the delivery port of enrichment ware (4) has outlet (102) of box (1) to pass through the pipeline intercommunication.

2. The rapid ballast water monitoring device according to claim 1, wherein a V1 electromagnetic valve (105) is arranged on a pipeline between the positive pressure pump (104) and the enriching device (4), a water outlet of the positive pressure pump (104) is communicated with a water inlet of the V1 electromagnetic valve (105) through a pipeline, a water outlet of the V1 electromagnetic valve (105) is communicated with a water inlet of the enriching device (4) through a pipeline, and a pressure regulating pipeline is arranged on a pipeline between the positive pressure pump (104) and the V1 electromagnetic valve (105).

3. The rapid ballast water monitoring device according to claim 2, wherein the pressure regulating pipeline comprises a pressure regulating valve (106), a pipeline between the positive pressure pump (104) and the V1 electromagnetic valve (105) is communicated with a water inlet of the pressure regulating valve (106) through a pipeline, and a water outlet of the pressure regulating valve (106) is communicated with a water outlet (102) of the tank body (1) through a pipeline.

4. A rapid ballast water monitoring device according to claim 3, wherein the tank (1) is provided with an air inlet (103), and the tank (1) is provided with an evacuation pipeline which is matched with the air inlet (103) and is used for discharging all the residual ballast water in the enriching device (4).

5. The rapid ballast water monitoring device according to claim 4, wherein the emptying pipeline comprises a V2 electromagnetic valve (107), an input port of the V2 electromagnetic valve (107) is communicated with an air inlet (103) of the tank body (1) through a pipeline, an output port of the V2 electromagnetic valve (107) is communicated with a pipeline between the V1 electromagnetic valve (105) and the enriching device (4), a negative pressure pump (108) is arranged on the pipeline between the enriching device (4) and a water outlet (102) of the tank body (1), a water outlet of the enriching device (4) is communicated with a water inlet of the negative pressure pump (108) through a pipeline, and a water outlet of the negative pressure pump (108) is communicated with a water inlet of the tank body (1) through a pipeline.

6. The quick monitoring device for ballast water according to claim 1, wherein the enriching device (4) comprises a shell (401), the shell (401) is of a tubular structure with a hollow inner cavity and open upper and lower ends, an A filter element (402) is arranged inside the enriching device (4), the A filter element (402) is of a tubular structure with a hollow inner cavity and open upper and lower ends, the outer diameter of the A filter element (402) is smaller than the inner diameter of the shell (401), the center line of the shell (401) coincides with the center line of the A filter element (402), the top end of the shell (401) is fixedly provided with an upper cover (403) for closing the top opening part of the shell (401) and the top opening part of the A filter element (402), an upper connector (404) which is communicated with the inner cavity of the shell (401) and is convenient for water inflow is arranged on the upper cover (403), a lower cover (405) for closing the bottom opening part of the shell (401) and the bottom opening part of the A filter element (402) is fixed at the bottom end of the shell (401), and a lower connector (406) which is convenient for water drainage is arranged on the lower cover (405).

7. The rapid ballast water monitoring device according to claim 6, wherein the joint of the upper cover (403) and the housing (401), the joint of the upper cover (403) and the A filter element (402), the joint of the lower cover (405) and the housing (401) and the joint of the lower cover (405) and the A filter element (402) are provided with O-rings (407) for improving the sealing performance.

8. The rapid ballast water monitoring device according to claim 1, wherein the enriching device (4) comprises an outer cover (408), the inner cavity of the outer cover (408) is hollow, an upper end pipe (409) communicated with the inner cavity of the outer cover (408) is arranged at the middle position of the top end of the outer cover (408), a B filter element (410) is fixed at the middle position of the bottom of the inner cavity of the outer cover (408), the center line of the B filter element (410) coincides with the center line of the outer shell (401), the outer diameter of the B filter element (410) is smaller than the diameter of the inner cavity of the outer cover (408), the inner cavity of the B filter element (410) is hollow, and a lower end pipe (411) communicated with the inner cavity of the B filter element (410) is fixed at the middle position of the bottom end of the outer cover (408).