CN220079095U - Three-dimensional tissue culture device - Google Patents
Three-dimensional tissue culture device Download PDFInfo
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- CN220079095U CN220079095U CN202321565875.7U CN202321565875U CN220079095U CN 220079095 U CN220079095 U CN 220079095U CN 202321565875 U CN202321565875 U CN 202321565875U CN 220079095 U CN220079095 U CN 220079095U
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- stress
- cabin
- clamp
- connecting rod
- rod
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- 239000000463 material Substances 0.000 claims abstract description 10
- 238000010146 3D printing Methods 0.000 claims description 3
- 230000000007 visual effect Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 108010066057 cabin-1 Proteins 0.000 description 8
- 239000012528 membrane Substances 0.000 description 7
- 238000004113 cell culture Methods 0.000 description 6
- 238000001727 in vivo Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 230000000451 tissue damage Effects 0.000 description 3
- 231100000827 tissue damage Toxicity 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004264 monolayer culture Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a three-dimensional tissue culture device which comprises a stress cabin, a connecting rod and a clamp, wherein the connecting rod is L-shaped, a cross rod of the connecting rod is used for connecting an external motor, the tail end of a vertical rod of the connecting rod is connected with the clamp, the clamp is used for clamping a tissue sample or a material, the top of the stress cabin is provided with an opening, the vertical rod of the connecting rod and the clamp are placed in the stress cabin, and the tops of a pair of side walls of the stress cabin are provided with notches for placing the cross rod of the connecting rod; a hatch is also included for closing the stress hatch top opening. In the three-dimensional tissue culture device, the connecting rod is L-shaped, the clamp is arranged at the tail end of the vertical rod of the connecting rod, the transverse rod of the connecting rod is driven by the external power source to transversely move to drive the clamp to move to apply stress to a tissue sample, the position of the clamp is lower than that of the transverse rod, the transverse rod at the movable part is not soaked in culture solution, the problem of liquid leakage can be thoroughly solved, the waste of the culture solution is reduced, and the scientific research cost is reduced.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a three-dimensional tissue culture device.
Background
The three-dimensional cell culture technology is a technology between monolayer cell culture and animal experiments, not only can simulate the in-vivo environment to the greatest extent, but also can show the advantages of intuitiveness and condition controllability of cell culture. The conventional cell culture gradually loses the original properties due to the proliferation of cells in an in-vitro changed environment, often does not coincide with in-vivo conditions, and animal experiments are completely carried out in vivo, but the conventional cell culture is complicated due to the restriction of various factors in vivo and the mutual influence of in-vivo and external environments, so that a single process is difficult to study, and an intermediate process is difficult to study.
The common three-dimensional tissue culture device has a structure similar to a cell culture dish disclosed in Chinese patent No. 202881283U and capable of providing traction type mechanical stress, and comprises a base dish and an elastic silica gel membrane culture surface capable of being stretched, wherein two ends of the culture surface are detachably fixed on a moving block, two moving block clamp fixation culture surfaces are positioned in the base dish, a threaded hole is formed in the moving block, a threaded rod can be screwed in the threaded hole, a knob is arranged at the tail end of the threaded rod, the threaded rod can pass through an upper hole of the base dish, and the knob is positioned outside the base dish. In the actual experiment, the whole basal dish (stress cabin) can be filled with culture solution, needs to soak whole movable block (anchor clamps) completely, and the upper hole that supplies the threaded rod to pass is had to the basal dish both sides, and the threaded rod can be moved in the upper hole and be exerted stress for the culture face, and the sealing performance of upper hole is not good can lead to the culture solution in the basal dish to spill from this hole, often needs frequent replenishment culture solution in the experimental process, and some experiments need 24 hours overnight, just have not to have in time to replenish, and the culture solution is expensive, increases scientific research cost. In addition, when installing a bio-stress membrane or tissue, it is necessary to install it in a base plate, and since the operation space is too small, it takes a long time to install it, and it is very easy to cause the problem of cell shedding or tissue damage on the stress membrane.
Disclosure of Invention
In order to solve the defects in the prior art, the utility model provides a three-dimensional tissue culture device which can avoid the leakage of culture solution and save the cost.
In order to achieve the technical purpose, the utility model adopts the following technical scheme:
the three-dimensional tissue culture device comprises a stress cabin, a connecting rod and a clamp, wherein the connecting rod is L-shaped, a cross rod of the connecting rod is used for connecting an external motor, the tail end of a vertical rod of the connecting rod is connected with the clamp, the clamp is used for clamping a tissue sample or a material, the top of the stress cabin is opened, the vertical rod of the connecting rod and the clamp are placed in the stress cabin, and a notch for placing the cross rod of the connecting rod is formed in the top of a pair of side walls of the stress cabin; a hatch is also included for closing the stress hatch top opening.
Further, the support frame is connected with the outside of the stress cabin corresponding to the notch area, and the support frame is provided with a groove matched with the cross rod of the connecting rod.
Further, the top of the cabin cover is provided with two clamp placement limiting grooves which are oppositely arranged.
Further, the limiting groove comprises a fixing groove and a sliding groove, the fixing groove is a U-shaped opening towards the sliding groove, the inner dimension of the fixing groove is matched with the outer dimension of the clamp, the sliding groove comprises a pair of clamping ribs which are arranged in parallel, and the distance between the two clamping ribs is equal to the width dimension of the clamp.
Further, connecting end lugs are arranged on two sides of the bottom of the stress cabin, and connecting holes are formed in the connecting end lugs.
Further, the connecting hole is in a strip shape.
Further, at least one side wall of the stress cabin is provided with a transparent visual window.
Further, the stress cabin is integrally formed by 3D printing of POM materials.
Compared with the prior art, the utility model has the beneficial effects that:
in the three-dimensional tissue culture device, the connecting rod is L-shaped, the clamp is arranged at the tail end of the vertical rod, the clamp is driven to move by the transverse rod to move, and the transverse rod at the moving part is not soaked in the culture solution, so that the problem of liquid leakage can be thoroughly solved, the waste of the culture solution is reduced, and the scientific research cost is reduced.
In the three-dimensional tissue culture device, the clamp placement limit groove is formed in the top of the hatch cover, the hatch cover is also a clamp mounting bracket, a stress membrane or tissue can be aseptically mounted in the biosafety cabinet, and then the hatch cover and the clamp mounting bracket are directly placed into the stress cabin together, so that the operation is simple and quick, the error rate is low, and meanwhile, cell shedding and tissue damage on the stress membrane can be prevented.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic overall structure of an embodiment of the present utility model;
FIG. 2 is a schematic cross-sectional view of FIG. 1;
fig. 3 is a schematic view of the hatch as a clamp mounting bracket.
Reference numerals: the device comprises a stress cabin, a 11-connecting lug, a 12-connecting hole, a 13-visual window, a 2-connecting rod, a 21-cross rod, a 22-vertical rod, a 3-clamp, a 4-support frame, a 5-cabin cover, a 51-fixing groove and a 52-sliding groove.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. The components of the embodiments of the present utility model generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The three-dimensional tissue culture device comprises a stress cabin 1, a connecting rod 2 and a clamp 3, wherein the connecting rod 2 is L-shaped, a cross rod 21 of the connecting rod 2 is used for being connected with an external motor, the tail end of a vertical rod 22 of the connecting rod 2 is connected with the clamp 3, the clamp 3 is used for clamping a tissue sample or a material, the top of the stress cabin 1 is opened, the vertical rod 22 of the connecting rod 2 and the clamp 3 are placed in the stress cabin 1, and a notch for placing the cross rod 21 of the connecting rod 2 is formed in the top of a pair of side walls of the stress cabin 1; preferably, a supporting frame 4 is connected to the corresponding notch area outside the stress cabin 1, and the supporting frame 4 is provided with a groove matched with the cross rod 21 of the connecting rod 2; a hatch 5 is also included, said hatch 5 being intended to close the top opening of the stress chamber 1.
Further, the top of the hatch cover 5 is provided with two clamp placement limiting grooves which are oppositely arranged. Specifically, the limiting groove comprises a fixing groove 51 and a sliding groove 52, the fixing groove 51 is provided with a U-shaped opening towards the sliding groove 52, the inner dimension of the fixing groove 51 is matched with the outer dimension of the clamp 3, the sliding groove 52 comprises a pair of clamping ribs which are arranged in parallel, and the distance between the two clamping ribs is equal to the width dimension of the clamp 3; as shown in fig. 3, when installing the tissue sample, the clamps 3 installed on the connecting rod 2 can be placed in the limiting grooves, and then the tissue sample is installed, and one side of the clamps can slide in the sliding grooves 52 to adjust the distance between the two clamps 3, so as to adapt to the tissue samples with different lengths.
The two sides of the bottom of the stress cabin 1 are provided with connecting end lugs 11, and the connecting end lugs 11 are provided with long strip-shaped connecting holes 12, so that the stress cabin 1 can be conveniently fixed on external equipment through fasteners.
The stress cabin is integrally formed by 3D printing of POM materials, the interior of the stress cabin is smooth and easy to clean, and the POM materials are high-temperature and high-pressure resistant; preferably, the stress cabin 1 is provided with a transparent visual window 13, so that the internal condition can be conveniently observed.
The connecting rod is L-shaped, the clamp is arranged at the tail end of the vertical rod of the connecting rod, the transverse rod of the connecting rod is driven by an external power source to transversely move to drive the clamp to move to apply stress to a tissue sample or a material, the position of the clamp is lower than that of the transverse rod, the transverse rod at the movable part is not soaked in culture solution, the problem of liquid leakage can be thoroughly solved, the waste of the culture solution is reduced, and the cost of scientific research is reduced. According to the utility model, the clamp placing limiting groove is formed in the top of the hatch cover, and the hatch cover is also a clamp mounting bracket, so that a stress membrane or a tissue sample or other materials can be aseptically mounted in the biosafety cabinet and then directly placed into the stress cabin together, the operation is simple and quick, the error rate is low, and meanwhile, the cell falling off and tissue damage on the stress membrane can be prevented.
Of course, the present utility model is capable of other various embodiments and its several details are capable of modification and variation in light of the present utility model by one skilled in the art without departing from the spirit and scope of the utility model as defined in the appended claims.
Claims (8)
1. The utility model provides a three-dimensional tissue culture device, includes stress cabin (1), connecting rod (2) and anchor clamps (3), its characterized in that: the connecting rod (2) is L-shaped, a cross rod (21) of the connecting rod (2) is used for connecting an external motor, the tail end of a vertical rod (22) of the connecting rod (2) is connected with a clamp (3), the clamp (3) is used for clamping a tissue sample or a material, the top of the stress cabin (1) is opened, the vertical rod (22) of the connecting rod (2) and the clamp (3) are placed in the stress cabin (1), and a gap for placing the cross rod (21) of the connecting rod (2) is formed in the top of a pair of side walls of the stress cabin (1); the device also comprises a hatch cover (5), wherein the hatch cover (5) is used for closing the top opening of the stress cabin (1).
2. The three-dimensional tissue culture device of claim 1, wherein: the stress cabin (1) is connected with a support frame (4) at the outer corresponding gap area, and the support frame (4) is provided with a groove matched with a cross rod (21) of the connecting rod (2).
3. The three-dimensional tissue culture device of claim 1, wherein: the top of the hatch cover (5) is provided with two clamp placement limiting grooves which are oppositely arranged.
4. The three-dimensional tissue culture device of claim 3, wherein: the limiting groove comprises a fixing groove (51) and a sliding groove (52), the fixing groove (51) is provided with a U-shaped opening towards the sliding groove (52), the inner dimension of the fixing groove (51) is matched with the outer dimension of the clamp (3), the sliding groove (52) comprises a pair of clamping ribs which are arranged in parallel, and the distance between the two clamping ribs is equal to the width dimension of the clamp (3).
5. The three-dimensional tissue culture device of claim 1, wherein: connecting end lugs (11) are arranged on two sides of the bottom of the stress cabin (1), and connecting holes (12) are formed in the connecting end lugs (11).
6. The three-dimensional tissue culture device of claim 5, wherein: the connecting hole (12) is in a strip shape.
7. The three-dimensional tissue culture device of claim 1, wherein: at least one side wall of the stress cabin (1) is provided with a transparent visual window (13).
8. The three-dimensional tissue culture device of claim 1 or 7, wherein: and the stress cabin is integrally formed by 3D printing of POM materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321565875.7U CN220079095U (en) | 2023-06-19 | 2023-06-19 | Three-dimensional tissue culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321565875.7U CN220079095U (en) | 2023-06-19 | 2023-06-19 | Three-dimensional tissue culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220079095U true CN220079095U (en) | 2023-11-24 |
Family
ID=88830921
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321565875.7U Active CN220079095U (en) | 2023-06-19 | 2023-06-19 | Three-dimensional tissue culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220079095U (en) |
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2023
- 2023-06-19 CN CN202321565875.7U patent/CN220079095U/en active Active
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