CN220063959U - System for rapidly determining enrofloxacin residue in animal-derived food - Google Patents
System for rapidly determining enrofloxacin residue in animal-derived food Download PDFInfo
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- CN220063959U CN220063959U CN202320627279.0U CN202320627279U CN220063959U CN 220063959 U CN220063959 U CN 220063959U CN 202320627279 U CN202320627279 U CN 202320627279U CN 220063959 U CN220063959 U CN 220063959U
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- Prior art keywords
- sample
- animal
- enrofloxacin
- pretreatment
- residue
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- 235000013305 food Nutrition 0.000 title claims abstract description 16
- SPFYMRJSYKOXGV-UHFFFAOYSA-N Baytril Chemical group C1CN(CC)CCN1C(C(=C1)F)=CC2=C1C(=O)C(C(O)=O)=CN2C1CC1 SPFYMRJSYKOXGV-UHFFFAOYSA-N 0.000 title claims abstract description 15
- 241001465754 Metazoa Species 0.000 title claims abstract description 15
- 229960000740 enrofloxacin Drugs 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 21
- 238000002347 injection Methods 0.000 claims description 13
- 239000007924 injection Substances 0.000 claims description 13
- 229910052757 nitrogen Inorganic materials 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- 238000007664 blowing Methods 0.000 claims description 3
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 3
- 230000007613 environmental effect Effects 0.000 claims description 2
- 239000000273 veterinary drug Substances 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 5
- 238000000746 purification Methods 0.000 abstract description 4
- 239000011159 matrix material Substances 0.000 abstract description 3
- 238000002203 pretreatment Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 4
- 241000282414 Homo sapiens Species 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000001819 mass spectrum Methods 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 1
- XBJFCYDKBDVADW-UHFFFAOYSA-N acetonitrile;formic acid Chemical compound CC#N.OC=O XBJFCYDKBDVADW-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229960003405 ciprofloxacin Drugs 0.000 description 1
- 238000007872 degassing Methods 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- HQVFCQRVQFYGRJ-UHFFFAOYSA-N formic acid;hydrate Chemical compound O.OC=O HQVFCQRVQFYGRJ-UHFFFAOYSA-N 0.000 description 1
- 238000000622 liquid--liquid extraction Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- LISFMEBWQUVKPJ-UHFFFAOYSA-N quinolin-2-ol Chemical compound C1=CC=C2NC(=O)C=CC2=C1 LISFMEBWQUVKPJ-UHFFFAOYSA-N 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The utility model relates to the technical field of food detection, and aims to provide a system for rapidly determining the amount of enrofloxacin residue in animal-derived food, which comprises a sample pretreatment system and a sample detection system, wherein the sample pretreatment system comprises a sample pulverizer, a sample collector, a sample homogenizer, a sample extractor and a sample concentrator, and the sample detection system comprises a computer module, a degasser, a sample injector, a quantitative ring and a mass spectrometer, and has the following effects: the pretreatment method disclosed by the utility model is simple in operation, easy to control, low in cost, less in material loss to be detected, capable of accurately detecting the enrofloxacin residue in animal-derived foods, wide in application range, strong in matrix interference resistance, capable of effectively solving the problems of complex analysis and pretreatment of veterinary drug residues, long in purification time, unobvious purification effect, high in cost and the like, and good in economic benefit and wide in market prospect.
Description
Technical Field
The utility model relates to the technical field of food detection, in particular to a measuring system for rapidly measuring enrofloxacin residue in animal-derived foods.
Background
The enrofloxacin serving as the third-generation artificially synthesized quinolone medicine has the characteristics of broad-spectrum antibacterial property, strong bactericidal property, small toxic and side effects, low price and the like, and has good application prospect as shown by pharmacodynamics and pharmacokinetics of the enrofloxacin, can be widely applied to the treatment of diseases such as terrestrial and aquatic animals and the like, and has good effect.
Because the phenomenon of disorder and abuse of veterinary drug residues such as enrofloxacin and a metabolite ciprofloxacin is common, the residual quantity of the veterinary drug residues in an animal body exceeds the relevant national standard, and if the veterinary drug residues are used as human animal-derived foods, the veterinary drug residues are potentially harmful to human beings, so that the detection of the veterinary drug residues is very necessary for guaranteeing the safety of the animal-derived foods. The detection pretreatment and detection of enrofloxacin in the prior animal-derived food are finished by various and complex sample pretreatment equipment and detection instruments, and the method has the characteristics of complex operation, low efficiency, high detection cost, high instrument maintenance cost and the like.
Disclosure of Invention
The utility model aims to provide a measuring system for rapidly measuring enrofloxacin residue in animal-derived foods, which aims to solve the problems in the background technology.
The technical scheme adopted by the utility model is as follows:
the system for rapidly determining the determination of the enrofloxacin residue in animal-derived foods comprises a sample pretreatment system and a sample detection system, wherein the sample pretreatment system comprises a sample crusher, a sample collector, a sample homogenizer, a sample leacher and a sample concentrator, and the sample detection system comprises a computer module, a degasser, a sample injector, a quantitative ring and a mass spectrometer.
The sample homogenizer includes a homogenizer and an ice bath controller.
The sample extractor comprises a sample purifier and a refrigerated centrifuge, wherein the sample purifier is used for isolated collection of supernatant.
The sample concentrator is a nitrogen blowing instrument which is connected with a nitrogen generator and is used for drying and concentrating the leached supernatant.
The computer module comprises a sample injection controller, a condition controller, data processing software and a display.
The mass spectrometer is connected with a nitrogen generator which is used for drying and atomizing the substances to be tested.
In summary, due to the adoption of the technical scheme, the beneficial effects of the utility model are as follows:
the pretreatment method disclosed by the utility model is simple in operation, easy to control, low in cost, less in material loss to be detected, capable of accurately detecting the enrofloxacin residue in animal-derived foods, wide in application range, strong in matrix interference resistance, capable of effectively solving the problems of complex analysis and pretreatment of veterinary drug residues, long in purification time, unobvious purification effect, high in cost and the like, and good in economic benefit and wide in market prospect.
Drawings
FIG. 1 is a schematic diagram of the system principle of an embodiment of the present utility model.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. The components of the embodiments of the present utility model generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
As shown in FIG. 1, the system for rapidly determining the residual enrofloxacin in animal-derived foods comprises a sample pretreatment system and a sample detection system, wherein the sample pretreatment system comprises a sample pulverizer, a sample collector, a sample homogenizer, a sample extractor and a sample concentrator, the sample homogenizer comprises a homogenizer and an ice bath controller, and the ice bath controller is used for controlling ice bath environmental conditions; the sample extractor comprises a sample purifier and a refrigerated centrifuge, wherein the sample purifier is used for isolating and collecting supernatant; the sample concentrator is a nitrogen blowing instrument which is connected with a nitrogen generator and is used for drying and concentrating the leached supernatant; the sample detection system comprises a computer module, a degasser, a sample injector, a quantitative ring and a mass spectrometer, wherein the computer module comprises a sample injection controller, a condition controller, data processing software and a display, the computer module is used for controlling the sample injection amount and the sample injection condition and displaying the sample injection condition through the display, the mass spectrometer is connected with a nitrogen generator, and the nitrogen generator is used for drying and atomizing substances to be detected in the mass spectrometer.
The working principle of the utility model is as follows: weighing about 5g of the treated sample, placing the sample in a polypropylene centrifuge tube, accurately adding 20.0mL of 2% acetonitrile formate solution, shearing and homogenizing at high speed under ice bath environment, so that the sample can be maintained in a good physical and chemical state, the substance to be detected is not easily damaged, taking out the centrifuge tube, controlling and maintaining the temperature of the cavity of the centrifuge tube at about 10 ℃ for centrifugation, and repeating the above operations to combine the supernatants.
Transferring a certain amount of n-hexane and acetonitrile into a separating funnel, mixing the two solvents, vigorously shaking, standing until two phases are obviously layered after liquid-liquid extraction, taking the upper liquid and transferring the upper liquid into a new extractor, pouring the supernatant in the previous step into the new extractor, vigorously shaking, degassing, taking care of leakage prevention, degreasing for the purpose, standing and collecting the lower layer. Drying in nitrogen blower, constant volume of primary water, filtering with 0.22 μm water filter head, placing into sample injection vial of liquid chromatograph, sucking 10 μl of concentrated leached liquid with sample injection needle, introducing into quantitative ring in liquid injection system, wherein the initial ratio of mobile phase is 10% acetonitrile and 90% 0.1 formic acid water solution, passing through C of ultra-high performance liquid chromatograph 18 The chromatographic column is separated at a column temperature of 35 ℃ and a flow rate of 0.25 min/mL. The substances to be detected enter a mass spectrum detector after being separated, the mass spectrum conditions are that spraying voltage is 3650V, sheath air pressure is 35Arb, auxiliary air pressure is 10Arb, capillary temperature is 310 ℃, atomizer temperature is 360 ℃, and a nitrogen generator is used as a dryer and an atomizer in a mass spectrometer.
The pretreatment method has the advantages of simple operation, easy control, low cost and less loss of substances to be detected, can accurately detect the residual amount of enrofloxacin in animal-derived foods, and has wide application range and strong matrix interference resistance.
Claims (1)
1. A system for rapidly determining the residual amount of enrofloxacin in animal-derived foods is characterized in that: the sample pretreatment system comprises a sample grinder, a sample collector, a sample homogenizer, a sample leacher and a sample concentrator which are connected in sequence, and the sample detection system comprises a computer module, a degasser, a sample injector, a quantitative ring and a mass spectrometer which are connected in a telecommunication way;
the sample homogenizer comprises a homogenizer and an ice bath controller, wherein the ice bath controller is used for controlling ice bath environmental conditions;
the sample extractor comprises a sample purifier and a refrigerated centrifuge, wherein the sample purifier is used for isolating and collecting supernatant;
the sample concentrator is a nitrogen blowing instrument which is connected with a nitrogen generator and is used for drying and concentrating the leached supernatant;
the computer module comprises a sample injection controller, a condition controller, data processing software and a display which are connected in a telecommunication way, and is used for controlling the sample injection quantity and the sample injection condition and displaying the sample injection quantity and the sample injection condition through the display;
the mass spectrometer is connected with a nitrogen generator which is used for drying and atomizing the substances to be tested.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320627279.0U CN220063959U (en) | 2023-03-27 | 2023-03-27 | System for rapidly determining enrofloxacin residue in animal-derived food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320627279.0U CN220063959U (en) | 2023-03-27 | 2023-03-27 | System for rapidly determining enrofloxacin residue in animal-derived food |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220063959U true CN220063959U (en) | 2023-11-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320627279.0U Active CN220063959U (en) | 2023-03-27 | 2023-03-27 | System for rapidly determining enrofloxacin residue in animal-derived food |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220063959U (en) |
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2023
- 2023-03-27 CN CN202320627279.0U patent/CN220063959U/en active Active
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