CN220056883U - Culture bottle capable of preventing cells from agglomerating - Google Patents
Culture bottle capable of preventing cells from agglomerating Download PDFInfo
- Publication number
- CN220056883U CN220056883U CN202321389088.1U CN202321389088U CN220056883U CN 220056883 U CN220056883 U CN 220056883U CN 202321389088 U CN202321389088 U CN 202321389088U CN 220056883 U CN220056883 U CN 220056883U
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- CN
- China
- Prior art keywords
- suction nozzle
- bottle body
- bottle
- opening
- wall
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 239000002245 particle Substances 0.000 claims abstract description 11
- 238000003825 pressing Methods 0.000 claims abstract description 5
- 230000002776 aggregation Effects 0.000 claims description 11
- 238000005054 agglomeration Methods 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 238000004220 aggregation Methods 0.000 claims 5
- 238000004113 cell culture Methods 0.000 description 4
- 239000012930 cell culture fluid Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000007664 blowing Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000003116 impacting effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a culture bottle for preventing cells from agglomerating, which comprises a suction nozzle and a bottle body; the suction nozzle is positioned in the bottle body, the opening of the tip of the suction nozzle is gradually reduced, the tail end of the suction nozzle is adhered with the connecting end into a whole, a small hole is formed in the wall, close to the bottle body, of the suction nozzle, the connecting end is fixed on the outer wall of the bottle body, the connecting end is provided with an opening, an air bag is connected to the opening, the breaking net is sleeved outside the tip of the suction nozzle through a fixing ring in a pressing mode, and a particle plate is arranged at the bottom of the bottle body below the suction nozzle; the bottle body is flat, and one side of the bottle body is inclined upwards and is provided with a bottle cap.
Description
Technical Field
The utility model belongs to the technical field of cell culture flasks, and particularly relates to a culture flask capable of preventing cells from agglomerating.
Background
Cell-binding phenomenon is often encountered when performing cell culture experiments, particularly when detecting the formation rate of cell clones, laying monoclonal antibodies, electrotransformation, etc., and if the reliability of the results obtained by cell-binding is reduced, the performance of the experiments will be affected. The existing cell culture bottle has a simple structure and can not effectively prevent the problem of cell agglomeration.
Disclosure of Invention
The utility model aims to solve the defects in the prior art, and provides a culture bottle for preventing cells from agglomerating.
In order to achieve the above purpose, the present utility model provides the following technical solutions: a culture bottle for preventing cell agglomeration comprises a suction nozzle and a bottle body; the suction nozzle is positioned in the bottle body, the opening of the tip of the suction nozzle is gradually reduced, the tail end of the suction nozzle is adhered with the connecting end into a whole, a small hole is formed in the wall, close to the bottle body, of the suction nozzle, the connecting end is fixed on the outer wall of the bottle body, the connecting end is provided with an opening, an air bag is connected to the opening, the breaking net is sleeved outside the tip of the suction nozzle through a fixing ring in a pressing mode, and a particle plate is arranged at the bottom of the bottle body below the suction nozzle; the bottle body is flat, and one side of the bottle body is inclined upwards and is provided with a bottle cap.
Preferably, the number of the suction nozzles is more than or equal to 3, and the suction nozzles are respectively positioned on three outer vertical surfaces of the bottle body.
Preferably, the nozzle tip opening is 1-5mm higher than the bottom inside the body.
Preferably, the air bag is a silica gel bag, and the bottom of the air bag is adhered to the connecting end wall through a pressing ring.
Preferably, the opening of the connecting end is connected with the filter screen through the fixed end.
Preferably, the particle board is provided with wavy bulges.
Compared with the prior art, the utility model has the beneficial effects that: firstly, the design is simple and reasonable, the manufacturing cost is low, and the popularization and the use are easy; secondly, dispersing cells by blowing and sucking, particularly dispersing the cells after breaking the net, and combining the cells by impacting the particle plate so that the cells are not agglomerated; thirdly, the operation of the non-contact culture solution can prevent secondary pollution caused by blowing and sucking by the instrument.
Other features of the present disclosure and its advantages will become apparent from the following detailed description of exemplary embodiments of the disclosure, which proceeds with reference to the accompanying drawings.
Drawings
In order to more clearly illustrate the embodiments of the present disclosure or the technical solutions in the related art, the drawings that are required to be used in the embodiments or the related technical descriptions will be briefly described below, and it is apparent that the drawings in the following description are only embodiments of the present disclosure, and other drawings may be obtained from the provided drawings without inventive effort to those of ordinary skill in the art.
FIG. 1 is a schematic view of the whole culture flask of the present utility model;
FIG. 2 is a schematic view of a portion of a flask according to the utility model.
In the figure: 1. suction nozzle, 2, body, 3, bottle lid, 4, link, 5, particle board, 6, gasbag, 7, broken net, 8, solid fixed ring, 9, filter screen, 10, solid fixed end, 11, pressfitting circle, 12, aperture.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the utility model, its application, or uses. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The relative arrangement of the components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present utility model unless it is specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective parts shown in the drawings are not drawn in actual scale for convenience of description. Techniques, methods, and apparatus known to one of ordinary skill in the relevant art may not be discussed in detail, but should be considered part of the specification where appropriate. In all examples shown and discussed herein, any specific values should be construed as merely illustrative, and not a limitation. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further discussion thereof is necessary in subsequent figures.
Spatially relative terms, such as "above … …," "above … …," "upper surface at … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial location relative to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "above" or "over" other devices or structures would then be oriented "below" or "beneath" the other devices or structures. Thus, the exemplary term "above … …" may include both orientations of "above … …" and "below … …". The device may also be positioned in other different ways (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.
Referring to fig. 1 and 2, the present utility model provides a technical solution: a culture bottle for preventing cell agglomeration comprises a suction nozzle 1 and a bottle body 2; the suction nozzle 1 is positioned in the bottle body 2, the number of the suction nozzles 1 is 3, the suction nozzles 1 are respectively positioned in the middle parts of three outer vertical surfaces of the bottle body 2, the opening of the tip end of the suction nozzle 1 is 2mm higher than the inner bottom of the bottle body 2, the opening of the tip end of the suction nozzle 1 is gradually reduced, the tail end of the suction nozzle 1 is adhered to the connecting end 4 into a whole, small holes 12 are formed in the wall, close to the bottle body 2, of the suction nozzle 1, the connecting end 4 is fixed on the outer wall of the bottle body 2, the connecting end 4 is provided with an opening, an air bag 6 is connected with a filter screen 9 through a fixed end 10, the air bag 6 is a silica gel bag, the bottom of the air bag 6 is adhered to the wall of the connecting end 4 through a pressing ring 11, the outer end of the suction nozzle 1 is pressed and sleeved with a crushing net 7 through a fixed ring 8, a particle plate 5 is arranged at the bottom of the bottle body 2 below the suction nozzle 1, and wavy bulges are arranged on the particle plate 5; the bottle body 2 is flat, and one side of the bottle body 2 is inclined upwards and is provided with a bottle cap 3.
Working principle: in the cell culture process, 3 air bags 6 are respectively gently and slightly pinched, then the air bags 6 are slowly rebounded, the operation action cannot be too large, so that cell breakage is prevented, cell culture fluid firstly enters the suction nozzle 1 through the crushing net 7, is blocked and separated after contacting the crushing net 7 when being agglomerated cells, the air bags 6 are pinched again, the cell culture fluid is blown to the particle board 5 after passing through the crushing net 7, is further separated after being collided by friction of wavy bulges on the particle board 5, the cell agglomeration rate is minimized by two means, the cell agglomeration can be greatly reduced through repeated operation, the experiment is ensured to be smoothly carried out, and after the air bags 6 are finally loosened, the small holes 12 are communicated with the interior of the bottle body 2, and the residual cell culture fluid falls back into the bottle body 2 through gravity.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a prevent blake bottle of cell agglomeration, includes suction nozzle (1) and body (2), its characterized in that: the utility model discloses a bottle, including bottle body (2), suction nozzle (1), fixed ring (8), broken net (7), nozzle (1) tip is equipped with on the wall of bottle body (2), suction nozzle (1) tip opening is located in bottle body (2), suction nozzle (1) end and link (4) are glued as an organic wholely, suction nozzle (1) is close to be equipped with aperture (12) on the wall of bottle body (2), link (4) are fixed in bottle body (2) outer wall, be equipped with opening on it and be connected with gasbag (6), suction nozzle (1) tip is outside through solid fixed ring (8) press-fit broken net (7), bottle (2) bottom below suction nozzle (1) is equipped with particle board (5); the bottle body (2) is flat, and one side of the bottle body (2) is inclined upwards and is provided with a bottle cap (3).
2. A cell aggregation preventing culture flask according to claim 1, wherein: the number of the suction nozzles (1) is more than or equal to 3, and the suction nozzles are respectively positioned on three outer vertical surfaces of the bottle body (2).
3. A cell aggregation preventing culture flask according to claim 1, wherein: the tip opening of the suction nozzle (1) is 1-5mm higher than the inner bottom of the bottle body (2).
4. A cell aggregation preventing culture flask according to claim 1, wherein: the air bag (6) is a silica gel bag, and the bottom of the air bag (6) is adhered to the wall of the connecting end (4) through a pressing ring (11).
5. A cell aggregation preventing culture flask according to claim 1, wherein: the opening of the connecting end (4) is connected with a filter screen (9) through a fixed end (10).
6. A cell aggregation preventing culture flask according to claim 1, wherein: the particle board (5) is provided with wavy bulges.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321389088.1U CN220056883U (en) | 2023-06-02 | 2023-06-02 | Culture bottle capable of preventing cells from agglomerating |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321389088.1U CN220056883U (en) | 2023-06-02 | 2023-06-02 | Culture bottle capable of preventing cells from agglomerating |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220056883U true CN220056883U (en) | 2023-11-21 |
Family
ID=88764659
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321389088.1U Active CN220056883U (en) | 2023-06-02 | 2023-06-02 | Culture bottle capable of preventing cells from agglomerating |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220056883U (en) |
-
2023
- 2023-06-02 CN CN202321389088.1U patent/CN220056883U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant |