CN220027028U

CN220027028U - Polypeptide preparation purification device

Info

Publication number: CN220027028U
Application number: CN202321580558.2U
Authority: CN
Inventors: 董远; 陈颂; 王道锋; 李东
Original assignee: Henan Qianze Biotechnology Co ltd
Current assignee: Henan Qianze Biotechnology Co ltd
Priority date: 2023-06-20
Filing date: 2023-06-20
Publication date: 2023-11-17
Anticipated expiration: 2033-06-20

Abstract

The utility model relates to the technical field of polypeptide preparation equipment, in particular to a polypeptide preparation and purification device, which comprises a tank body, wherein a sealing cover is arranged at the top of the tank body, an opening and closing mechanism is arranged between the sealing cover and the tank body, a mounting box is fixedly arranged at the top of the sealing cover, driving motors are fixedly arranged at the left side and the right side of the inside of the mounting box through fixing pieces, a mixing bin, a machine bin and a processing bin are respectively arranged in the tank body, a transmission rod is vertically arranged in the mixing bin, a round rod and a U-shaped rod are respectively fixedly arranged at the outer side of the transmission rod, a scraping plate is fixedly arranged at one side of the U-shaped rod far away from the transmission rod, and an electric push rod is fixedly arranged at the left side of the inside of the machine bin.

Description

Polypeptide preparation purification device

Technical Field

The utility model relates to the technical field of polypeptide preparation equipment, in particular to a polypeptide preparation and purification device.

Background

In the purification of polypeptides, purification equipment is required, but the existing purification equipment is to convey a protein base solution and a reaction solution into a tank body to react to generate polypeptides, and then purify the polypeptides by using a filtering device.

The utility model discloses a polypeptide preparation and purification device used at present, and the utility model of patent application number CN202222925066.4 discloses polypeptide preparation and purification equipment, belonging to the technical field of polypeptide preparation. The utility model comprises a shell, a feeding pipe, a discharging valve, an auxiliary frame and a selective permeable membrane, wherein the right end of the shell is respectively communicated with the left ends of the feeding pipe and the discharging valve, the feeding pipe is positioned at the top of the discharging valve, the inside of the shell is respectively fixedly connected with the surfaces of the auxiliary frame and the selective permeable membrane, the auxiliary frame is positioned at the top of the selective permeable membrane, the top of the shell is fixedly connected with a piston cylinder, the output end of the piston cylinder extends to the inside of the shell and is fixedly connected with an auxiliary plate, and the top of the auxiliary plate is fixedly connected with a motor positioned outside the piston cylinder. According to the utility model, the piston cylinder, the auxiliary plate, the sealing cover and the auxiliary frame are arranged, so that the mixed solution which is not reacted well is prevented from penetrating through the selective permeable membrane, the protein base solution and the reaction solution are mixed by utilizing the motor and the stirring rod, the reaction speed is accelerated, and the production efficiency of the purification equipment is improved.

Although the above patent solves the problems in the background art, the following disadvantages still exist: 1. the shell in the device adopts an integrated structure, and the shell does not have opening and closing property, so that the interior of the shell cannot be comprehensively checked when the device is used, and meanwhile, the stirring rod cannot be maintained, so that the follow-up purification operation is not facilitated; 2. the device does not have the function of cleaning the inner wall of the shell, so that reactants are easy to remain on the inner wall of the shell during the purification reaction, thereby bringing trouble to cleaning staff, reducing the cleanliness of the device and being not beneficial to the subsequent reaction operation between protein base solution and reaction solution.

In summary, the present utility model solves the above-mentioned problems of the prior art by designing a device for purifying polypeptide preparation.

Disclosure of Invention

The present utility model is directed to a device for purifying polypeptide, which solves the above-mentioned problems of the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

the utility model provides a polypeptide preparation purification device, includes the jar body, the bottom of the jar body is fixed mounting respectively has the supporting leg, the outside of the jar body is fixed import, transparent strip and export respectively, the top of the jar body is provided with sealed lid, be provided with the mechanism that opens and shuts between sealed lid and the jar body, the top fixed mounting of sealed lid has the mounting box, the inside left and right sides of mounting box all is provided with driving motor through the mounting is fixed, mixing bin, machine storehouse and processing storehouse have been seted up respectively to the inside of the jar body, mixing bin's inside is vertical to be provided with the transfer line, the outside of transfer line is fixed mounting respectively has round bar and U-shaped pole, one side that the transfer line was kept away from to the U-shaped pole is fixed to be provided with the scraper blade, the inside left side fixed mounting of machine storehouse has the electric putter No. one, the right-hand member of electric putter is fixed to be provided with the push pedal, the inside left and right sides fixed mounting of processing storehouse has the filter plate.

The opening and closing mechanism comprises a lower supporting block, an upper supporting block, a groove and a protruding block, wherein the lower supporting block is fixedly arranged on the outer side surface of the tank body, the top surface of the lower supporting block is fixedly provided with a second electric push rod, the upper supporting block is fixedly arranged on the outer side surface of the sealing cover, the groove is formed in the side surface of the top of the tank body, and the top surface of the protruding block is fixedly connected with the bottom surface of the sealing cover.

As a preferable scheme of the utility model, the inlet is communicated with the interior of the mixing bin, the top of the mixing bin is provided with a sealing plug, the transparent strip is embedded and arranged on the outer side surface of the tank body, and the right end of the transparent strip extends to the interior of the mixing bin.

As a preferable scheme of the utility model, the surface of the bottom end of the inside of the treatment bin is obliquely arranged, the outlet is communicated with the inside of the treatment bin, and a valve is arranged on the outer side of the outlet.

As a preferable scheme of the utility model, the bottom of the mixing bin is provided with a falling opening, wherein the falling opening penetrates through the inner wall of the bin and extends to the surface of the top end of the inside of the processing bin, and the outer side surface of the push plate is in sealing fit with the periphery of the inner wall of the bin.

As a preferable scheme of the utility model, the bottom end of the inside of the installation box and the top surface of the sealing cover are positioned in the same plane, the output end of the driving motor is fixedly connected with the top end of the transmission rod, the round rods are distributed annularly and symmetrically relative to the outer side surface of the transmission rod, and one side of the scraping plate far away from the U-shaped rod is contacted with the inner wall of the mixing bin.

As a preferable scheme of the utility model, the lower supporting blocks are respectively positioned at the front side and the rear side of the tank body, and the top of the telescopic end of the second electric push rod is welded with the bottom surface of the upper supporting block.

As a preferred embodiment of the present utility model, the cross-sectional shape of the groove is adapted to the bump.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the polypeptide preparation and purification device, the sealing cover and the tank body are separated by utilizing the structural design in the opening and closing mechanism, so that the purpose of opening the tank body is achieved, a worker can conveniently check the inside of the tank body, and meanwhile, the round rod is convenient to maintain subsequently, and the follow-up purification operation is facilitated.

2. According to the polypeptide preparation and purification device, the structural design of the driving motor, the transmission rod, the U-shaped rod and the scraping plate is utilized, so that the scraping plate is in movable contact with the inner wall of the tank body, the purpose of cleaning the inner wall of the tank body is achieved, convenience is brought to cleaning staff, cleanliness of the inside of the tank body is guaranteed, and reaction operation between subsequent protein base liquid and reaction solution is facilitated.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the present utility model;

FIG. 2 is a schematic structural view of the opening and closing mechanism of the present utility model;

fig. 3 is a schematic view of a partial cross-sectional structure of the device of the present utility model.

In the figure: 1. a tank body; 101. support legs; 102. an inlet; 103. a transparent strip; 104. an outlet; 105. sealing cover; 106. a mounting box; 107. a driving motor; 108. a mixing bin; 1081. a transmission rod; 1082. a round bar; 1083. a U-shaped rod; 1084. a scraper; 109. a machine cabin; 1091. a first electric push rod; 1092. a push plate; 110. a treatment bin; 1101. a filter plate; 2. an opening and closing mechanism; 201. a lower support block; 202. an upper support block; 203. a groove; 204. a bump; 205. no. two electric putter.

Detailed Description

The technical solutions of the embodiments of the present utility model will be clearly and completely described below in conjunction with the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, but not all embodiments, and all other embodiments obtained by those skilled in the art without making any inventive effort based on the embodiments of the present utility model are within the scope of protection of the present utility model.

In order that the utility model may be readily understood, several embodiments of the utility model will be described more fully hereinafter with reference to the accompanying drawings, in which, however, the utility model may be embodied in many different forms and is not limited to the embodiments described herein, but instead is provided for the purpose of providing a more thorough and complete disclosure of the utility model.

It will be understood that when an element is referred to as being "mounted" on another element, it can be directly on the other element or intervening elements may also be present, and when an element is referred to as being "connected" to the other element, it may be directly connected to the other element or intervening elements may also be present, the terms "vertical", "horizontal", "left", "right" and the like are used herein for the purpose of illustration only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs, and the terms used herein in this description of the utility model are for the purpose of describing particular embodiments only and are not intended to be limiting of the utility model, with the term "and/or" as used herein including any and all combinations of one or more of the associated listed items.

Referring to fig. 1-3, the present utility model provides a technical solution:

the utility model provides a polypeptide preparation purification device, including jar body 1, the bottom of jar body 1 is fixed mounting respectively has supporting leg 101, jar body 1's outside is fixed respectively and is provided with import 102, transparent strip 103 and export 104, jar body 1's top is provided with sealed lid 105, sealed lid 105's top fixed mounting has mounting box 106, the inside left and right sides of mounting box 106 is all fixed through the mounting and is provided with driving motor 107, mix storehouse 108 has been seted up respectively in jar body 1's inside, machine storehouse 109 and processing storehouse 110, mix storehouse 108's inside vertically and be provided with transfer line 1081, transfer line 1081's outside is fixed mounting respectively has round bar 1082 and U-shaped pole 1083, U-shaped pole 1083 is fixed and is provided with scraper 1084 in one side of keeping away from transfer line 1081, electric putter 1091's right-hand member is fixed and is provided with push pedal 1092 in the inside left side of machine storehouse 109 is fixed and is provided with filter plate 1101 in the inside left and right sides of processing storehouse 110 is fixed and is provided with filter plate 1101;

specifically, a dropping opening is formed in the bottom of the mixing bin 108, wherein the dropping opening penetrates through the inner wall of the machine bin 109 and extends to the inner top surface of the treatment bin 110, and the outer side surface of the push plate 1092 is in sealing fit with the periphery of the inner wall of the machine bin 109;

in this embodiment, the setting of the first electric putter 1091 is mainly used for pushing the push plate 1092, so that the push plate 1092 and the machine cabin 109 are in sliding fit, thereby achieving the function of opening and closing the falling port, and effectively preventing the unreacted protein base solution and the reaction solution from falling into the treatment cabin 110 through the falling port.

Specifically, the bottom end of the inside of the installation box 106 and the top surface of the sealing cover 105 are located in the same plane, the output end of the driving motor 107 is fixedly connected with the top end of the driving rod 1081, the round rod 1082 is symmetrically distributed in a ring shape with respect to the outer side surface of the driving rod 1081, and one side of the scraping plate 1084 away from the U-shaped rod 1083 is contacted with the inner wall of the mixing bin 108;

in this embodiment, the driving motor 107 is mainly used to drive the driving rod 1081 to rotate, and the driving rod 1081 can drive the round rod 1082, the U-shaped rod 1083 and the scraper 1084 to rotate, so that the reaction time between the protein base solution and the reaction solution is shortened, the cleaning of the inner wall of the tank body 1 is facilitated, and the practical effect of the device is improved.

Preferably, the inlet 102 is communicated with the interior of the mixing bin 108, a sealing plug is arranged at the top of the inlet, the transparent strip 103 is embedded and arranged on the outer side surface of the tank body 1, the right end of the transparent strip extends to the interior of the mixing bin 108, the bottom end surface of the interior of the processing bin 110 is obliquely arranged, the outlet 104 is communicated with the interior of the processing bin 110, and a valve is arranged on the outer side of the outlet.

In this embodiment, referring to fig. 1, 2 and 3, an opening and closing mechanism 2 is disposed between the sealing cover 105 and the can body 1, the opening and closing mechanism 2 includes a lower supporting block 201, an upper supporting block 202, a groove 203 and a protruding block 204, the lower supporting block 201 is fixedly mounted on the outer side surface of the can body 1, the top surface of the lower supporting block is fixedly provided with a second electric push rod 205, the upper supporting block 202 is fixedly mounted on the outer side surface of the sealing cover 105, the groove 203 is opened on the top side surface of the can body 1, and the top surface of the protruding block 204 is fixedly connected with the bottom surface of the sealing cover 105;

specifically, the lower supporting blocks 201 are respectively positioned at the front side and the rear side of the tank body 1, the top of the telescopic end of the second electric push rod 205 is welded with the bottom surface of the upper supporting block 202, and the cross section shape of the groove 203 is matched with the convex block 204;

in this embodiment, the setting of No. two electric putter 205 mainly is used for driving the supporting shoe 202 and carries out vertical removal, and goes up the supporting shoe 202 and can drive sealed lid 105 and remove, and wherein the adaptation setting of recess 203 and lug 204 can be convenient for sealed lid 105 and the top surface laminating contact of jar body 1 to reached the effect of opening and shutting to jar body 1, satisfied user's demand.

The working flow of the utility model is as follows: when the device for preparing and purifying the polypeptide is used, firstly, the device is moved to a required position and the bottom surface of the supporting leg 101 is ensured to be in full contact with the ground, secondly, protein base solution and reaction solution are added into the mixing bin 108, the sealing plug is pulled out to sequentially connect the protein base solution output pipe and the reaction solution output pipe with the inlet 102, the sealing plug can be reset after the addition is finished, finally, the reaction operation between the protein base solution and the reaction solution can be carried out, in the process of carrying out the reaction operation on the protein base solution and the reaction solution, the driving motor 107 is started, the output end of the driving motor 107 drives the transmission rod 1081 to rotate, the transmission rod 1081 drives the round rod 1082, the U-shaped rod 1083 and the scraping plate 1084 to rotate, and the protein base solution and the reaction solution are mixed in an accelerating way under the stirring action of the round rod 1082, the scraper 1084 is in moving contact with the inner wall of the tank body 1 in the rotating process, so that the reactant on the inner wall of the tank body 1 is scraped, when the reaction between the protein base solution and the reaction solution is completed, the first electric push rod 1091 is started to gradually shrink, the generated polypeptide solution gradually falls into the treatment bin 110 through the falling port and passes through the filter plate 1101 along with the separation of the push plate 1092 from the falling port, the polypeptide solution filtered by the filter plate 1101 is discharged from the outlet 104, further, when the inside of the tank body 1 is required to be checked and the round rod 1082 is required to be checked, the second electric push rod 205 is started, the telescopic end of the second electric push rod 205 starts to stretch and drives the upper support block 202 to vertically move upwards, the sealing cover 105 moves upwards along with the upward movement of the upper support block 202, the inside of the tank body 1 is opened along with the separation of the round rod 1082 from the tank body 1, from this the staff of being convenient for look over the inside of jar body 1 comprehensively and to the inspection operation of round bar 1082, satisfied staff's demand.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the spirit and scope of the utility model as defined by the appended claims and their equivalents.

Claims

1. The utility model provides a polypeptide preparation purification device, includes jar body (1), its characterized in that: the filter plate filter is characterized in that supporting legs (101) are fixedly arranged at the bottom of the tank body (1) respectively, an inlet (102), a transparent strip (103) and an outlet (104) are fixedly arranged at the outer side of the tank body (1) respectively, a sealing cover (105) is arranged at the top of the tank body (1), an opening and closing mechanism (2) is arranged between the sealing cover (105) and the tank body (1), a mounting box (106) is fixedly arranged at the top of the sealing cover (105), driving motors (107) are fixedly arranged at the left side and the right side of the inside of the mounting box (106) through fixing pieces, a mixing bin (108), a machine bin (109) and a processing bin (110) are respectively arranged at the inner side of the tank body (1), a transmission rod (1081) is vertically arranged at the inner side of the mixing bin (108), a round rod (1082) and a U-shaped rod (1083) are respectively fixedly arranged at the outer side of the transmission rod (1081), a scraper (1084) is fixedly arranged at one side of the sealing cover (105), an electric push plate (1091) is fixedly arranged at the left side and the right side of the electric push plate (1091), and a filter plate (1091) is fixedly arranged at the left side and the electric push plate (1091) is fixedly arranged at the two sides of the filter plate (1091;

the opening and closing mechanism (2) comprises a lower supporting block (201), an upper supporting block (202), a groove (203) and a protruding block (204), wherein the lower supporting block (201) is fixedly arranged on the outer side surface of the tank body (1), a second electric push rod (205) is fixedly arranged on the top surface of the lower supporting block, the upper supporting block (202) is fixedly arranged on the outer side surface of the sealing cover (105), the groove (203) is formed in the side surface of the top of the tank body (1), and the top surface of the protruding block (204) is fixedly connected with the bottom surface of the sealing cover (105).

2. The apparatus for producing and purifying a polypeptide according to claim 1, wherein: the inlet (102) is communicated with the inside of the mixing bin (108), a sealing plug is arranged at the top of the mixing bin, the transparent strip (103) is embedded and arranged on the outer side surface of the tank body (1), and the right end of the transparent strip extends to the inside of the mixing bin (108).

3. The apparatus for producing and purifying a polypeptide according to claim 1, wherein: the surface of the bottom end of the inside of the treatment bin (110) is obliquely arranged, the outlet (104) is communicated with the inside of the treatment bin (110), and a valve is arranged on the outer side of the outlet.

4. The apparatus for producing and purifying a polypeptide according to claim 1, wherein: the bottom of mixing bin (108) is provided with the whereabouts mouth, and wherein the whereabouts mouth runs through in the inner wall of quick-witted storehouse (109) and extends to the inside top surface of handling storehouse (110), the outside surface of push pedal (1092) is sealed laminating all around with the inner wall of quick-witted storehouse (109).

5. The apparatus for producing and purifying a polypeptide according to claim 1, wherein: the inside bottom of install bin (106) is located the coplanar with the top surface of sealed lid (105), the output of driving motor (107) and the top fixed connection of transfer line (1081), round bar (1082) are annular symmetric distribution about the outside surface of transfer line (1081), one side that U-shaped pole (1083) was kept away from to scraper blade (1084) is contacted with the inner wall of mixing bin (108).

6. The apparatus for producing and purifying a polypeptide according to claim 1, wherein: the lower supporting blocks (201) are respectively positioned at the front side and the rear side of the tank body (1), and the top of the telescopic end of the second electric push rod (205) is welded and connected with the bottom surface of the upper supporting block (202).

7. The apparatus for producing and purifying a polypeptide according to claim 1, wherein: the cross-sectional shape of the groove (203) is matched with the convex block (204).