CN220002385U - Integrated system for lipid nanoparticle synthesis and dialysis - Google Patents

Abstract

The utility model provides an integrated system for synthesis and dialysis of lipid nanoparticles, and relates to the technical field of nanoparticle preparation equipment, comprising a mixing unit, a moving unit and a plurality of groups of dialysis sites; the mixing unit comprises a mixing device and a discharge pipeline, the mixing device is communicated with any dialysis site through the discharge pipeline, and the movement unit drives an outlet of the discharge pipeline to move from one dialysis site to the other dialysis site. The outlet of the discharge pipeline is driven to move from one dialysis site to the other dialysis site through the movement unit, so that the mixing device can be respectively communicated with the plurality of dialysis sites, multi-site collection of the synthesized solvent is facilitated, and the collected synthesized solvent is dialyzed by the finished product plate, so that the synthesized solvent is prevented from deteriorating when the synthesized solvent is placed for a long time.

Description

Integrated system for lipid nanoparticle synthesis and dialysis

Technical Field

The utility model relates to the technical field of nanoparticle preparation equipment, in particular to an integrated system for synthesis and dialysis of lipid nanoparticles.

Background

Lipid Nanoparticles (LNPs) are novel drug delivery systems that use biocompatible lipid materials as carriers to dissolve, encapsulate, or attach drugs or other biologically active substances to the surface of lipid nanoparticles. The lipid nanoparticle can improve drug absorption, has the advantages of slow release, controlled release, drug stability improvement, curative effect enhancement, toxic and side effects reduction and the like, and is stable in organisms and in storage processes. The vector system is widely applied to medicines such as gene medicines, antitumor medicines, proteins, polypeptides and the like.

The prior Chinese patent application document with the publication number of CN115700142A discloses a device and a method for preparing lipid nano-particles, wherein the device comprises: a lipid solution source; an auxiliary solution source; a microfluidic mixer coupled to the lipid solution source via a first fluid channel having a first fluid valve to operably receive lipid solution from the lipid solution source and to the auxiliary solution source via a second fluid channel having a second fluid valve to operably receive auxiliary solution from the auxiliary solution source; wherein the microfluidic mixer further has a fluid mixing conduit configured to cause orderly mixing of the lipid solution and the auxiliary solution therein and self-assembly of lipids in the lipid solution into lipid nanoparticles; a controller coupled to the first and second fluid valves to control the fluid flow characteristics permitted by the first and second fluid valves, respectively; and a lipid nanoparticle container fluidly coupled to the microfluidic mixer and configured to receive the formed lipid nanoparticles from the microfluidic mixer.

When the lipid nanoparticle preparation system in the prior art performs multiple synthesis operations, multi-site collection of the synthesized solvent is difficult, and when long-time mixing is performed, the mixed solution is easy to deteriorate after long-time placement, and the solution needs to be improved.

Disclosure of Invention

In view of the drawbacks of the prior art, an object of the present utility model is to provide an integrated system for synthesis and dialysis of lipid nanoparticles.

The utility model provides an integrated system for synthesis and dialysis of lipid nanoparticles, which comprises a mixing unit, a movement unit and a plurality of groups of dialysis sites; the mixing unit comprises a mixing device and a discharge pipeline, the mixing device is communicated with any dialysis site through the discharge pipeline, and the movement unit drives an outlet of the discharge pipeline to move from one dialysis site to the other.

Preferably, the discharging pipeline comprises a mixing pipe and a sample discharging head, the discharging hole of the mixing device is positioned at the lower end of the mixing device, the upper end of the mixing pipe is communicated with the discharging hole of the mixing device, and the lower end of the mixing pipe is communicated with the sample discharging head.

Preferably, the mixing device comprises a T-joint comprising two opposed feed ports and one discharge port, the mixing tube being in communication with the discharge port of the T-joint.

Preferably, two feed inlets of the T-shaped joint are respectively communicated with a feed pipeline before mixing.

Preferably, the sample ejection head comprises a needle, and any of the dialysis sites is covered with a sealing membrane, and any of the sealing membranes allows the sample ejection head to puncture.

Preferably, the device further comprises a workbench, wherein a finished board is detachably arranged on the workbench, and the finished board comprises a plurality of hole sites to form a plurality of dialysis sites.

Preferably, a second adapter is fixedly arranged on the workbench, and an opening for accommodating a finished board is arranged on the second adapter, and the finished board is embedded in the opening of the second adapter.

Preferably, the workbench is further provided with a waste liquid tank, and the motion unit drives the outlet of the discharge pipeline to move to the waste liquid tank, or drives the outlet of the discharge pipeline to be separated from the waste liquid tank.

Preferably, the moving unit includes: first linear motion mechanism: driving the outlet of the discharge pipeline to reciprocate up and down along the vertical direction; and a second linear motion mechanism: driving the workbench to reciprocate in a linear motion along a first direction in a horizontal plane; and a third linear motion mechanism: driving the workbench to reciprocate in a linear motion along a second direction in a horizontal plane; the first direction and the second direction are perpendicular to each other.

Preferably, the mixing device further comprises a frame and a housing, both the mixing unit and the moving unit being arranged on the frame; the shell covers the frame to form a working space, and any group of material taking sites and any group of cleaning sites are located in the working space.

Compared with the prior art, the utility model has the following beneficial effects:

1. the utility model drives the outlet of the discharge pipeline to move from one dialysis site to the other dialysis site through the movement unit, so that the mixing device can be respectively communicated with a plurality of dialysis sites, thereby being beneficial to realizing multi-site collection of the synthesized solvent and dialysis of the collected synthesized solvent, and being beneficial to ensuring that the synthesized solvent does not deteriorate when being placed for a long time.

2. According to the utility model, the cleaning liquid is obtained through the feeding pipeline before mixing, and is discharged into the waste liquid tank through the mixing device and the discharge pipeline in sequence to clean the mixing unit, so that the cross contamination of solvents for mixing operation twice before and after is reduced, and the mixing quality is improved.

3. According to the utility model, the hole sites of the first raw liquid plate and the second raw liquid plate, which are pre-provided with the solvent, are isolated from the external environment through the sealing film, so that the volatilization of the solvent is reduced.

Drawings

Other features, objects and advantages of the present utility model will become more apparent upon reading of the detailed description of non-limiting embodiments, given with reference to the accompanying drawings in which:

FIG. 1 is a schematic diagram of the overall structure of a lipid nanoparticle preparation system embodying the present utility model.

Reference numerals:

the first sampling head 1 and the second guide rail 19

Working table 20 of sample discharging head 2

First stock solution pipe 3 first stock solution plate 21

Second sampling head 4 first adapter 22

First Y-type switching valve 5 finished plate 23

First syringe 6 third adapter 24

Second injector 7 second stock solution plate 25

Second Y-type switching valve 8 second adapter 26

Second stock solution pipe 9 foot cup 27

Bottom plate 28 of T-joint 10

First drain pipe 11 mounting plate 29

Second drain 12 housing 30

First guide rail 31 of mixing tube 13

Third rail 32 of first cleaning tank 14

Third linear motion mechanism 33 of waste liquid tank 15

Back plate 34 of second cleaning tank 16

First linear motion mechanism 17 support column 35

Second linear motion mechanism 18 fixed block 36

Detailed Description

The present utility model will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the present utility model, but are not intended to limit the utility model in any way. It should be noted that variations and modifications could be made by those skilled in the art without departing from the inventive concept. These are all within the scope of the present utility model.

As shown in fig. 1, an integrated system for synthesis and dialysis of lipid nanoparticles according to the present utility model comprises a mixing unit and a movement unit, the movement unit providing a plurality of groups of dialysis sites to the mixing unit. The movement unit may first provide the mixing unit with a set of dialysis sites, the mixing unit performs the mixing operation and inputs the mixed material into the dialysis sites via the discharge line for the dialysis operation, and then the movement unit provides the mixing unit with another set of dialysis sites, the mixing unit performs the mixing operation again and inputs the mixed material into the dialysis sites via the discharge line for the dialysis operation.

The motion unit can provide a plurality of dialysis sites for the mixing unit successively, the mixing unit inputs the mixed materials into the dialysis sites after one-time material taking and mixing operation is completed, then the next material taking and mixing operation is continued, the mixed materials can be subjected to dialysis operation through the dialysis sites, and the situation that the mixed materials are placed for long-time placement to deteriorate in the continuous preparation process of lipid nano particles is realized.

The mixing unit comprises a first material taking device, a second material taking device and a mixing device, and the first material taking device and the second material taking device are respectively communicated with the mixing device. When the mixing unit performs the material taking and mixing operation, one possible implementation is as follows: under the control of the control unit, the first material taking device and the second material taking device firstly draw the solvent and then discharge the solvent into the mixing device.

Specifically, the first extracting device comprises a first injector 6, a first Y-shaped switching valve 5 and a first sampling head 1, the first Y-shaped switching valve 5 comprises three communication ports, an inlet and an outlet of the first injector 6 are communicated with the first communication port of the first Y-shaped switching valve 5, the second communication port of the first Y-shaped switching valve 5 is communicated with the first sampling head 1 through a first raw liquid pipe 3, and the third communication port of the first Y-shaped switching valve 5 is communicated with the mixing device through a first liquid discharge pipe 11. The first Y-switch valve 5 can control the first syringe 6 to communicate with the first sampling head 1 or the first syringe 6 to communicate with the mixing device.

The second extracting device comprises a second injector 7, a second Y-shaped switching valve 8 and a second sampling head 4, the second Y-shaped switching valve 8 comprises three communication ports, an inlet and an outlet of the second injector 7 are communicated with a first communication port of the second Y-shaped switching valve 8, a second communication port of the second Y-shaped switching valve 8 is communicated with the second sampling head 4 through a second stock solution pipe 9, and a third communication port of the second Y-shaped switching valve 8 is communicated with the mixing device through a second liquid discharge pipe 12. The second Y-switch valve 8 can control the second syringe 7 to communicate with the second sampling head 4 or the second syringe 7 to communicate with the mixing device.

The mixing device can be a microfluidic chip or a T-shaped connector 10, the T-shaped connector 10 is preferably adopted in the utility model, and the T-shaped connector 10 realizes mixing by utilizing a turbulence principle, so that the pollution risk caused by corrosion of the microfluidic chip can be avoided, and the encapsulation of lipid nanoparticles is facilitated. The T-joint 10 comprises two opposite feed inlets and one discharge outlet, and the diameters of the two feed inlet pipelines of the T-joint 10 are the same. Two feed inlets of the T-shaped joint 10 are respectively communicated with a feed pipeline before mixing, and the concrete steps are as follows: one feed port of the T-shaped joint 10 is communicated with the third communication port of the first Y-shaped switching valve 5 through the second liquid discharge pipe 12, and the other feed port of the T-shaped joint 10 is communicated with the third communication port of the second Y-shaped switching valve 8 through the first liquid discharge pipe 11. The discharge gate that T type connects 10 is located T type and connects 10's lower extreme, and the discharge gate that T type connects 10 is connected with the upper end of mixing tube 13, and the lower extreme of mixing tube 13 is connected with the head of discharging the appearance 2, and the material after mixing can be discharged through the head of discharging the appearance 2.

Further, the positions of the first material taking device, the second material taking device and the mixing device are relatively fixed, and the positions of the first sampling head 1, the second sampling head 4 and the discharging head 2 are relatively fixed, so that the mixing unit is an integrated device, and the overall motion control of the mixing unit is facilitated.

Still further, the mixing unit also includes a mounting plate 29, a linear drive mechanism, and a fixed block 36. The mounting panel 29 is vertical setting, and first extracting device, second extracting device, linear drive mechanism and mixing arrangement are all installed at its side of mounting panel 29, and fixed block 36 passes through support column 35 and mounting panel 29 fixed connection, and first sampling head 1, second sampling head 4 and the equal fixed mounting of stock form head 2 three are on fixed block 36. The two linear driving mechanisms are arranged and respectively drive the piston of the first injector 6 and the piston of the second injector 7 to move up and down along the vertical direction, so that the material taking or the material discharging of the first injector 6 and the second injector 7 are realized. The linear driving mechanism can be any structure capable of realizing linear reciprocating driving in the prior art and comprises a telescopic rod, a cylinder, a motor and a ball screw pair.

Still further, the mounting plate 29 is fixedly provided with the housing 30, the housing 30 is provided with two linear driving mechanisms, and the two linear driving mechanisms extend out of the housing 30 and are fixedly connected with the corresponding piston rod of the first syringe 6 or the corresponding piston rod of the second syringe 7, so that the two linear driving mechanisms are protected, the two linear driving mechanisms are prevented from being corroded, and the service lives of the two linear driving mechanisms are prolonged. It should be further noted that the start, close and movement of the two linear driving mechanisms are controlled by the control unit, so that the two linear driving mechanisms can move simultaneously and can also move in a time-sharing manner.

The motion unit provides multiple sets of take-off sites to the mixing unit. The motion unit provides a group of material taking sites for the mixing unit, then the mixing unit takes materials, then the motion unit provides a group of dialysis sites for the mixing unit, then the mixing operation is carried out, the mixed solution is input into the dialysis sites for the dialysis operation, then the motion unit provides another group of material taking sites for the mixing unit, the mixing unit takes materials again, then the motion unit provides another group of dialysis sites for the mixing unit, then the mixing operation is carried out, and the mixed solution is input into the dialysis sites for the dialysis operation.

The motion unit can provide a plurality of groups of material taking sites for the mixing unit successively, and the mixing unit can continue to carry out the next material taking and mixing operation after the completion of the one-time material taking and mixing operation, so that the continuous preparation of the lipid nano particles is realized for a plurality of times. The mixed solution is subjected to dialysis at the dialysis site, and the standing solution is deteriorated due to long-term standing.

The first Y-type switching valve 5 communicates the first syringe 6 with the first sampling head 1, the second Y-type switching valve 8 communicates the second syringe 7 with the second sampling head 4, the two linear driving mechanisms are simultaneously started and respectively drive the first syringe 6 and the second syringe 7 at the same given speed to extract a certain amount of solvent, then the first Y-type switching valve 5 communicates the first syringe 6 with the T-shaped joint 10, the second Y-type switching valve 8 communicates the second syringe 7 with the T-shaped joint 10, then the two linear driving mechanisms are simultaneously started and respectively drive the first syringe 6 and the second syringe 7 at the same given speed to input the solvent into the T-shaped joint 10 at the same speed, the two parts of solvent are mixed in the T-shaped joint 10 and the mixing pipe 13, and the mixed substance is discharged through the sample discharging head 2.

What needs to be further explained is: the volumes of the solutions obtained by both the first syringe 6 and the second syringe 7 may be the same or different. When mixing, the volume speed ratio of the materials conveyed to the mixing device by the first material taking device and the second material taking device is required to be the same, so that two solvents can reach the optimal mixing site in the middle of the T-shaped joint 10 at the same time, the two solvents can be ensured to be emptied in the same time period, and the mixing effect can be improved.

The multiple groups of material taking sites and multiple groups of dialysis sites are arranged on the workbench 20, the workbench 20 is horizontally placed, the motion unit drives the mixing unit to be close to or far away from the workbench 20 along the vertical direction, and the motion unit is driven to drive the workbench 20 to translate in a working plane. Any one of the sets of take-off points includes a first material point and a second material point. When the mixing unit moves to the material taking position, the first material taking device moves to the first material position, and the second material taking device simultaneously moves to the second material position. When the mixing unit leaves the material taking site, the first material taking device leaves the first material taking site, and the second material taking device leaves the second material taking site at the same time.

Specifically, the first stock solution plate 21 and the second stock solution plate 25 are placed on the workbench 20, a plurality of hole sites are respectively arranged on the first stock solution plate 21 and the second stock solution plate 25, and the solvent is preset in the hole sites, wherein the solvents in the hole sites can be the same or different. The hole site structure of the first stock solution plate 21 and the hole site number and structure of the second stock solution plate 25 are the same, the hole site on the first stock solution plate 21 forms a first material site, and the hole site on the second stock solution plate 25 forms a second material site. The hole site arrangement shapes on the first stock solution plate 21 and the second stock solution plate 25 may include regular patterns such as rectangular, circular, elliptical, etc., and the hole site arrangement shapes on the first stock solution plate 21 and the second stock solution plate 25 may also be irregular patterns. The number of the holes on the first stock solution plate 21 and the second stock solution plate 25 may be thirty-six holes or seventy-two holes, and it should be noted that the number of the holes on the first stock solution plate 21 and the second stock solution plate 25 is set according to actual requirements.

The positions of the first stock solution plate 21 and the second stock solution plate 25 on the workbench 20 are relatively fixed, so that the first material taking device and the second material taking device can take materials simultaneously, and the first stock solution plate 21 and the second stock solution plate 25 are mounted on the workbench 20: the first adapter 22 and the third adapter 24 are arranged on the workbench 20 in fixed positions, the first adapter 22 is provided with an opening for accommodating the first raw liquid plate 21, the third adapter 24 is provided with an opening for accommodating the second raw liquid plate 25, and the positions of the first raw liquid plate 21 and the second raw liquid plate 25 on the workbench 20 are relatively fixed by utilizing the two adapters in fixed positions on the workbench 20.

When taking materials, the first sampling head 1 stretches into a designated hole site on the first raw liquid plate 21, and the second sampling head 4 stretches into a designated hole site on the second raw liquid plate 25 corresponding to the designated hole site on the first raw liquid plate 21.

The workbench 20 is further provided with a finished product plate 23 detachably and fixedly arranged between the first raw liquid plate 21 and the second raw liquid plate 25 through a second adapter 26, the second adapter 26 is provided with an opening for accommodating the finished product plate 23, and the finished product plate 23 is embedded into the opening of the second adapter 26, so that the detachable positioning and mounting of the finished product plate 23 on the workbench 20 are realized. The finished board 23 of (2) comprises a plurality of finished hole sites, and when mixing, under the control of the control unit, the sample discharging head 2 extends into the appointed finished hole sites, and the mixed substances are input into the finished hole sites of the finished board 23. Further, the discharge head 2 comprises a needle, any dialysis site being covered with a closing membrane, any of said closing membranes allowing the discharge head 2 to puncture.

It should be noted that: the finished hole site on the finished plate 23 is the dialysis site of the utility model, and the solution after once mixing is input into one finished hole site, and as the finished plate 23 is placed in the second adapter 26, the solution in the dialysis site can be subjected to dialysis treatment, and when the screening quantity is large and the time is long, the solvent screened out at the beginning is not changed, so that high-throughput screening is realized. The finished plate 23 of the present utility model may also be a solution dialysis plate, and the dialysis may be completed by the solution dialysis plate after the mixed solution is inputted into a finished hole site. The utility model also allows the finished plate 23 to be removed from the second adapter 26, the lipid nanoparticles in the dialysis site to be dialyzed manually, and the finished plate 23 to be placed into the opening of the second adapter 26 after the dialysis is completed.

The motion unit provides more than one group of cleaning sites for the mixing unit, under the control of the control unit, the motion unit provides a group of taking sites for the mixing unit first, then the mixing unit takes materials, then the motion unit provides a group of dialysis sites for the mixing unit, the mixing unit performs mixing operation and inputs the mixed materials into the dialysis sites for dialysis operation through a discharge pipeline, then the motion unit provides a group of cleaning sites for the mixing unit, the mixing unit obtains cleaning liquid and cleans, then the motion unit provides another group of taking sites for the mixing unit, the mixing unit takes materials again, then the motion unit provides another group of dialysis sites for the mixing unit, and the mixing unit performs mixing operation and inputs the mixed materials into the dialysis sites for dialysis operation through a discharge pipeline.

Under the control of the control unit, the pipeline acquires the cleaning liquid before mixing of the mixing unit, then the cleaning liquid is input into the mixing pipeline of the mixing unit, and is discharged from the discharge pipeline of the mixing unit, namely, the first material taking device and the second material taking device firstly extract the cleaning liquid, and then the cleaning liquid is discharged into the mixing device and is discharged.

The cleaning operation is arranged between the two material taking and mixing operations, so that cross contamination of the solvents for the two material taking and mixing operations can be prevented, the mixing unit is cleaned by the cleaning liquid, the solvents for the last material taking and mixing operation attached to the pipe wall are removed, and the cross contamination of the solvents for the next material taking and mixing operation is avoided.

Specifically, the first cleaning tank 14 and the second cleaning tank 16 are fixedly arranged on the workbench 20, the positions of the first cleaning tank 14 and the second cleaning tank 16 on the workbench 20 are satisfied, when the first sampling head 1 stretches into the first cleaning tank 14, the second sampling head 4 stretches into the second cleaning tank 16 at the same time, and cleaning liquid is contained in both the first cleaning tank 14 and the second cleaning tank 16.

Under the control of the control unit, the first Y-type switching valve 5 enables the first injector 6 to be communicated with the first sampling head 1, the second Y-type switching valve 8 enables the second injector 7 to be communicated with the second sampling head 4, the two linear driving mechanisms are started simultaneously and respectively drive the first injector 6 and the second injector 7 at the same given speed to draw out a certain amount of cleaning liquid, then the first Y-type switching valve 5 enables the first injector 6 to be communicated with the T-shaped joint 10, the second Y-type switching valve 8 enables the second injector 7 to be communicated with the T-shaped joint 10, then the two linear driving mechanisms are started simultaneously and respectively drive the first injector 6 and the second injector 7 at the same given speed to input the cleaning liquid into the T-shaped joint 10, and the cleaning liquid is discharged through the sampling head 2 after sequentially passing through the T-shaped joint 10 and the mixing pipe 13.

Further, a waste liquid tank 15 is fixedly provided on the table 20, and the waste liquid tank 15 is used for receiving the cleaning liquid discharged from the discharge head 2, and in a possible embodiment, the waste liquid tank 15 is provided between the first cleaning tank 14 and the second cleaning tank 16. The movement unit drives the outlet of the discharge pipe to move to the waste liquid tank 15, or the movement unit drives the outlet of the discharge pipe to be separated from the waste liquid tank 15.

The movement unit comprises a first linear movement mechanism 17 and a second movement mechanism for driving the workbench 20 to translate in the horizontal plane, the translation of the workbench 20 in the horizontal plane comprises the reciprocating linear movement of the workbench 20 in the horizontal plane along a first direction, the reciprocating linear movement of the workbench 20 in the horizontal plane along a second direction, the first direction and the second direction are mutually perpendicular, and the first direction and the second direction are mutually perpendicular to the movement direction of the mixing unit. By mutually perpendicular directions of movement of the mixing unit, the first direction and the second direction, a mutual movement of both the mixing unit and the table 20 in space is achieved, whereby the movement unit provides the mixing unit with a take-off or cleaning site. It is to be noted that the movements of both the first linear motion mechanism 17 and the second motion mechanism are controlled by the control unit.

Specifically, the first linear motion mechanism 17 may adopt any linear reciprocating motion structure in the prior art, including a telescopic rod, a cylinder, an oil cylinder and a motor ball screw pair. It should be noted that the output shaft of the first linear motion mechanism 17 is vertically disposed, and the side surface of the mounting plate 29 facing away from the T-shaped joint 10 is fixedly connected with the output end of the first linear motion mechanism 17, so that the first linear motion mechanism 17 drives the mixing unit to reciprocate linearly in the vertical direction. Further, to improve the stability of the mixing unit movement, one possible implementation is: two first linear motion mechanisms 17 with parallel intervals are arranged and connected with the mounting plate 29, and the control unit controls the two first linear motion mechanisms 17 to drive the mounting plate 29 to move along the same direction at the same time. Another possible embodiment is: one or more groups of guide rails parallel to the movement direction of the first linear movement mechanism 17 are arranged on one side of the mounting plate 29 away from the T-shaped joint 10, and the mounting plate 29 is in sliding fit with any guide rail.

The second movement mechanism includes a second linear movement mechanism 18 that translates the table 20 in the first direction and a third linear movement mechanism 33 that translates in the second direction. The third linear motion mechanism 33 is disposed on the second linear motion mechanism 18, the table 20 is mounted on the third linear motion mechanism 33, the third linear motion mechanism 33 drives the table 20 to linearly reciprocate in the second direction, and the second linear motion mechanism 18 drives both the second linear motion mechanism 18 and the table 20 to linearly reciprocate in the first direction.

Both the second linear motion mechanism 18 and the third linear motion mechanism 33 may adopt any one of the prior art linear reciprocating motion structures including a telescopic rod, a cylinder, an oil cylinder, and a motor ball screw pair. Both the second linear motion mechanism 18 and the third linear motion mechanism 33 of the present utility model preferably employ a motor ball screw pair, and the movements of the second linear motion mechanism 18 and the third linear motion mechanism 33 are controlled by a control unit.

The control unit is used for presetting the position offset, and the motion unit is used for providing two groups of material taking sites for the mixing unit. The control unit drives the two groups of different material taking sites to the position right below the mixing unit by controlling the movement distance of the second linear movement mechanism 18 and the third linear movement mechanism 33, and the first linear movement mechanism 17 drives the mixing unit to move downwards for a certain distance, so that the movement unit can provide the two groups of material taking sites for the mixing unit successively. Further explaining: the control unit may employ a PLC control system. Similarly, the control unit presets the position offset, and the movement unit sequentially provides two groups of dialysis sites for the mixing unit.

The system for preparing the lipid nanoparticle also comprises a frame and a shell, wherein the mixing unit and the moving unit are both arranged on the frame. Specifically, the frame includes a back plate 34 and a bottom plate 28, the bottom plate 28 is horizontally arranged, the back plate 34 is vertically and fixedly installed on the bottom plate 28, the first linear motion mechanism 17 is fixedly installed on the back plate 34, the second linear motion mechanism 18 is fixedly installed on the bottom plate 28, and the back plate 34 does not interfere with the third linear motion mechanism 33.

In order to improve the stability of the second linear motion mechanism 18 driving the third linear motion mechanism 33 and the workbench 20 to move, the bottom plate 28 is provided with a first guide rail 31, and the length direction of the first guide rail 31 is parallel to the movement direction of the output end of the second linear motion mechanism 18. In order to improve the stability of the third linear motion mechanism 33 driving the workbench 20 to move, the first guide rail 31 is slidably provided with the third linear motion mechanism 33, the second guide rail 19 and the third guide rail 32, the first guide rail 31 is perpendicular to the second guide rail 19 and the third guide rail 32 respectively, the second guide rail 19 and the third guide rail 32 are arranged at intervals in parallel, and the length directions of the second guide rail 19 and the third guide rail 32 are parallel to the movement direction of the output end of the third linear motion mechanism 33.

The four corners of the bottom plate 28 are respectively and fixedly provided with a foot cup 27 for improving the stability of the whole placement of the equipment.

The housing houses the frame to form a working space, and any one set of material taking sites and any one set of cleaning sites are located in the working space. The housing covers the frame to form a working space, so that the corrosion of system equipment can be reduced, and the service life of the system can be prolonged.

In the description of the present utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

The foregoing describes specific embodiments of the present utility model. It is to be understood that the utility model is not limited to the particular embodiments described above, and that various changes or modifications may be made by those skilled in the art within the scope of the appended claims without affecting the spirit of the utility model. The embodiments of the utility model and the features of the embodiments may be combined with each other arbitrarily without conflict.

Claims (10)

1. An integrated system for synthesis and dialysis of lipid nanoparticles, which is characterized by comprising a mixing unit, a movement unit and a plurality of groups of dialysis sites;

the mixing unit comprises a mixing device and a discharge pipeline, the mixing device is communicated with any dialysis site through the discharge pipeline, and the movement unit drives an outlet of the discharge pipeline to move from one dialysis site to the other.

2. Integrated system for synthesis and dialysis of lipid nanoparticles according to claim 1, characterized in that the discharge line comprises a mixing tube (13) and a discharge head (2), the discharge opening of the mixing device being located at the lower end of the mixing device, the upper end of the mixing tube (13) being in communication with the discharge opening of the mixing device, the lower end of the mixing tube (13) being in communication with the discharge head (2).

3. Integrated system for synthesis and dialysis of lipid nanoparticles according to claim 2, characterized in that said mixing means comprise a T-joint (10), said T-joint (10) comprising two opposite feed ports and one discharge port, said mixing tube (13) being in communication with the discharge port of the T-joint (10).

4. An integrated system for synthesis and dialysis of lipid nanoparticles according to claim 3, characterized in that the two feed inlets of said T-joint (10) are connected to a respective feed line before mixing.

5. Integrated system for lipid nanoparticle synthesis, dialysis according to claim 2, characterized in that the discharge head (2) comprises a needle, any of the dialysis sites being covered with a closing membrane, any of the closing membranes allowing the discharge head (2) to puncture.

6. Integrated system for synthesis and dialysis of lipid nanoparticles according to claim 1, further comprising a table (20), on which table (20) a finished plate (23) is removably mounted, said finished plate (23) comprising a plurality of holes forming a plurality of dialysis sites.

7. Integrated system for synthesis and dialysis of lipid nanoparticles according to claim 6, characterized in that a second adapter (26) is fixedly arranged on said table (20), an opening for receiving a finished plate (23) is arranged on said second adapter (26), said finished plate (23) being embedded in the opening of the second adapter (26).

8. The integrated system for synthesis and dialysis of lipid nanoparticles according to claim 6, wherein said working table (20) is further provided with a waste liquid tank (15), said movement unit moves the outlet of the discharge line to the waste liquid tank (15), or said movement unit moves the outlet of the discharge line to be separated from the waste liquid tank (15).

9. The integrated system for lipid nanoparticle synthesis, dialysis as claimed in claim 6, wherein the movement unit comprises:

first linear motion mechanism (17): driving the outlet of the discharge pipeline to reciprocate up and down along the vertical direction;

a second linear motion mechanism (18): driving the workbench (20) to reciprocate in a linear motion along a first direction in a horizontal plane;

third linear motion mechanism (33): driving the workbench (20) to reciprocate in a linear motion along a second direction in a horizontal plane;

the first direction and the second direction are perpendicular to each other.

10. The integrated system for lipid nanoparticle synthesis, dialysis as claimed in claim 1, further comprising a frame and a housing, both the mixing unit and the movement unit being disposed on the frame;

the housing encloses the frame to form a working space.