CN219978087U - Flow cytometer sampler - Google Patents
Flow cytometer sampler Download PDFInfo
- Publication number
- CN219978087U CN219978087U CN202320759997.3U CN202320759997U CN219978087U CN 219978087 U CN219978087 U CN 219978087U CN 202320759997 U CN202320759997 U CN 202320759997U CN 219978087 U CN219978087 U CN 219978087U
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- China
- Prior art keywords
- sample
- flow cytometer
- temperature
- sampler
- temperature sensor
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- 238000010438 heat treatment Methods 0.000 claims abstract description 19
- 238000004321 preservation Methods 0.000 claims abstract description 10
- 238000011068 loading method Methods 0.000 claims description 17
- 238000007789 sealing Methods 0.000 claims description 8
- YKTSYUJCYHOUJP-UHFFFAOYSA-N [O--].[Al+3].[Al+3].[O-][Si]([O-])([O-])[O-] Chemical compound [O--].[Al+3].[Al+3].[O-][Si]([O-])([O-])[O-] YKTSYUJCYHOUJP-UHFFFAOYSA-N 0.000 claims description 3
- 239000010425 asbestos Substances 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 239000004744 fabric Substances 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 229910052895 riebeckite Inorganic materials 0.000 claims description 3
- 238000009413 insulation Methods 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 7
- 238000007689 inspection Methods 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 4
- 238000005485 electric heating Methods 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
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- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model relates to the technical field of biological inspection, in particular to a flow cytometer sampler which comprises a shell, wherein the top of the shell is open and is provided with an upper cover, a test tube rack and a temperature control assembly are arranged in the shell, a sample hole is formed in the test tube rack, a limiting pad is fixed in the sample hole, the limiting pad is annular, the temperature control assembly comprises a heating device and a refrigerating device, and opaque heat preservation layers are arranged on the inner walls of the shell and the upper cover. The temperature control assembly comprising the heating device and the refrigerating device is arranged to heat and refrigerate the inside of the shell, so that the device can be suitable for antibodies with different binding temperatures, and meanwhile, the limiting pad arranged at the sample hole can limit and fix the sample tube when being used for accommodating sample tubes with different sizes, so that the application range of the device is widened; the heat preservation not only provides the heat preservation effect, has still ensured simultaneously that the sample pipe of placing in the inside light interference of outside of casing.
Description
Technical Field
The utility model relates to the technical field of biological inspection, in particular to a flow cytometer sampler.
Background
Flow cytometry works on the principle of multi-parameter, rapid quantitative analysis of single cells or other biological particles by monoclonal antibodies at the cellular molecular level. The method can analyze tens of thousands of cells at high speed, can simultaneously measure a plurality of parameters from one cell, has the advantages of high speed, high precision and good accuracy, and is one of the most advanced cell quantitative analysis technologies in the current generation. It has become a hot spot for testing medical developments.
Before the cells enter the flow cytometer for detection, the cells need to be cultured first, a certain amount of the cells are placed in a culture tube (such as a flow tube or an EP tube), fluorescein coupled antibody is added for marking, and during the period, the temperature needs to be kept at room temperature, 4 ℃ or other temperature conditions according to the type of the antibody, and the cells are incubated in a dark place. Therefore, before loading the flow cytometer, temperature and light shielding conditions need to be ensured to ensure that the cells are effectively labeled so that accurate data can be measured.
The existing sample loading device, such as chinese patent document CN 217111717U (application No. 202220925012.5), discloses a low-temperature incubation sample loading device for preparing a flow cytometry sample, which comprises a housing, a cavity, an opaque upper cover, a test tube rack and an air compression refrigeration device, wherein the flow cytometry sample preparation operation is performed in a low-temperature environment, but the device can only provide a low-temperature culture environment, the test tube rack of the device is a double-layer test tube rack, the upper support is provided with a circular hole for placing a sample tube, and the sample tube with a smaller size (the minimum specification of an EP tube can reach 0.2 mL) cannot be effectively fixed due to the fixed size of the circular hole, so the application range of the device is extremely limited; meanwhile, if the flow tube with the size smaller than that of the circular hole is used, the sample tube and the circular hole are subjected to severe collision during rotation or vibration, liquid in the tube overflows slightly, the sample tube is damaged seriously, and serious pollution is caused by the fact that various control components (such as a temperature control device, a rotating device or a vibrating device) of the device are arranged below the test tube rack, so that the control components are easily corroded by the liquid during rotation or vibration, the experimental efficiency cannot be improved, and the operation and maintenance cost is increased excessively.
Disclosure of Invention
The utility model aims to overcome the defects of the prior art, and provides a flow cytometer sampler, which can be suitable for antibodies with different binding temperatures by arranging temperature control components capable of respectively realizing heating and cooling, and can be used for accommodating sample tubes with different sizes by arranging limiting pads at sample holes.
In order to achieve the technical effects, the utility model adopts the following technical scheme:
the utility model provides a flow cytometer sample loading ware, includes the casing, the casing top is uncovered and is equipped with the upper cover, the inside test-tube rack and the control by temperature change subassembly of being equipped with of casing, it has the sample hole to open on the test-tube rack, sample hole internal fixation has spacing pad, spacing pad is annular, the control by temperature change subassembly includes heating device and refrigerating plant, all be equipped with the light tight heat preservation on the inner wall of casing and upper cover.
According to the flow cytometer sample loading device, the temperature control assembly comprising the heating device and the refrigerating device is arranged, so that heating and refrigerating are realized inside the shell, the device can be suitable for antibodies with different binding temperatures, meanwhile, the limiting pad arranged at the sample hole can limit and fix the sample tube when being used for accommodating sample tubes with different sizes, and the application range of the device is widened; the heat preservation not only provides the heat preservation effect, has still ensured simultaneously that the sample pipe of placing in the inside light interference of outside of casing.
Preferably, the heat-insulating layer is made of any one of asbestos, aluminum silicate or ceramic fiber cloth.
Preferably, the temperature control assembly further comprises a controller and a temperature sensor, wherein the controller and the temperature sensor are both arranged inside the shell, the controller is respectively electrically connected with the temperature sensor, the heating device and the refrigerating device, and the controller is used for receiving the temperature measured by the temperature sensor and controlling the heating device and the refrigerating device according to the temperature.
Further preferably, a plurality of temperature sensors are provided, and the temperature sensors are uniformly fixed on the test tube rack.
Through setting up temperature sensor on the test-tube rack, the controller controls heating device and refrigerating plant according to each point temperature condition, after the settlement temperature, the device is inside to carry out automatically regulated according to the temperature, guarantees that the temperature is invariable.
Preferably, the limit pad is made of rubber, and the rubber has flexibility and certain toughness, so that the limit pad can still support and limit the sample tube after the sample tube is extruded.
Preferably, the top end of the shell is fixed with a sealing gasket, and a sealing groove which is correspondingly matched with the sealing gasket in position is arranged on the inner side of the upper cover, so that the internal environment and the outside in the airtight reaction process are isolated, external pollutants are prevented from entering, and the experimental result is influenced.
The utility model also provides a sample loading system for the flow cytometer, which comprises an oscillator and the sample loading device, wherein a working surface of the oscillator is provided with a fixed frame, the inner side of the fixed frame is provided with a limit groove, and the bottom of the outer wall of the shell of the sample loading device is fixed with a limit block matched with the limit groove.
According to the sample loading system provided by the utility model, the oscillator provides an oscillation mixing effect for the sample liquid in the sample tube, so that the sample tube can be uniformly mixed without being taken out, cell precipitation is prevented, and the sampling needle of the flow cytometer is blocked in the sampling process.
The utility model has the beneficial effects that:
1. the utility model provides a flow cytometer sample loading device, which is characterized in that a temperature control component comprising a heating device and a refrigerating device is arranged to heat and refrigerate the inside of a shell, so that the device can be suitable for antibodies with different binding temperatures, and meanwhile, a limiting pad arranged at a sample hole can limit and fix a sample tube when being used for accommodating sample tubes with different sizes, so that the application range of the device is widened; the heat preservation not only provides the heat preservation effect, has still ensured simultaneously that the sample pipe of placing in the inside light interference of outside of casing.
2. The utility model also provides a sample loading system for the flow cytometer, which provides an oscillation mixing effect for the sample liquid in the sample tube through the oscillator, can realize mixing without taking out the sample tube, prevents cell precipitation and causes the blockage of a sample injection needle of the flow cytometer in the sample injection process.
Drawings
FIG. 1 is a top view of a flow cytometer applicator as provided in example 1;
FIG. 2 is a top view of the loading system for a flow cytometer provided in example 2;
1, a shell; 11. an upper cover; 111. sealing grooves; 12. a sealing gasket; 13. a heat preservation layer; 14. a limiting block; 2. a test tube rack; 21. a sample hole; 22. a limit pad; 31. a heating device; 32. a refrigerating device; 33. a controller; 34. a temperature sensor; 4. an oscillator; 41. a working surface; 42. a fixed frame; 421. and a limit groove.
Detailed Description
The utility model will be further described with reference to examples and figures.
Example 1:
the utility model provides a flow cytometer sample loading ware, is shown as fig. 1, includes casing 1, casing 1 top is open and is equipped with upper cover 11, casing 1 top is fixed with sealed pad 12, the upper cover 11 inboard is equipped with and seals up the seal groove 111 that pad 12 position corresponds the matching, guarantees that the internal environment is isolated with the outside in the airtight reaction process, prevents that external pollutant from getting into, influences experimental result. The inner walls of the shell 1 and the upper cover 11 are respectively provided with an opaque heat-insulating layer 13, and the heat-insulating layers 13 are made of any one of asbestos, aluminum silicate or ceramic fiber cloth.
The inside test-tube rack 2 and the control by temperature change subassembly of being equipped with of casing 1, open there is sample hole 21 on the test-tube rack 2, sample hole 21 internal fixation has spacing pad 22, spacing pad 22 is the annular, spacing pad 22 is made by rubber, and rubber has certain toughness when having flexibility, can still play the support spacing effect to the sample pipe after the sample pipe extrusion.
The temperature control component comprises a heating device 31 and a refrigerating device 32, and in the embodiment, the heating device 31 can use a commercial electric heating tube, such as an M16/220V-1.5KW electric heating tube manufactured by Wen Xin electric heating appliance Co., ltd; the refrigeration device 32 may be a commercially available vortex tube, such as a VC62008G type vortex tube manufactured by Shanghai parasitic inferior electro-mechanical die technology, inc., the above types are examples only, and those skilled in the art may choose depending on the actual temperature and space size requirements. The temperature control assembly further comprises a controller 33 and a temperature sensor 34, the controller 33 and the temperature sensor 34 are both arranged inside the shell 1, the controller 33 is respectively electrically connected with the temperature sensor 34, the heating device 31 and the refrigerating device 32, and the controller 33 is used for receiving the temperature measured by the temperature sensor 34 and controlling the heating device 31 and the refrigerating device 32 according to the temperature. The temperature sensors 34 are provided in plurality, and the temperature sensors 34 are uniformly fixed on the test tube rack 2. By arranging the temperature sensor 34 on the test tube rack 2, the controller 33 controls the heating device 31 and the refrigerating device 32 according to the temperature conditions of each point, and after the temperature is set, the inside of the device can be automatically adjusted according to the temperature, so that the temperature is ensured to be constant.
Example 2:
the utility model provides a loading system for flow cytometer, as shown in fig. 2, includes oscillator 4 and above-mentioned loading ware, be equipped with fixed frame 42 on the working face 41 of oscillator 4, the fixed frame 42 inboard is opened there is spacing groove 421, the casing 1 outer wall bottom of loading ware be fixed with spacing groove 421 matched stopper 14.
Claims (6)
1. The utility model provides a flow cytometer sample loading ware, its characterized in that, includes casing (1), casing (1) top is uncovered and is equipped with upper cover (11), inside test-tube rack (2) and the control by temperature change subassembly of being equipped with of casing (1), it has sample hole (21) to open on test-tube rack (2), sample hole (21) internal fixation has spacing pad (22), spacing pad (22) are annular, the control by temperature change subassembly includes heating device (31) and refrigerating plant (32), all be equipped with opaque heat preservation (13) on the inner wall of casing (1) and upper cover (11).
2. Flow cytometer sampler according to claim 1 is characterized in that the thermal insulation layer (13) is made of any of asbestos, aluminum silicate or ceramic fiber cloth.
3. The flow cytometer sampler of claim 1 wherein the temperature control assembly further comprises a controller (33) and a temperature sensor (34), the controller (33) and the temperature sensor (34) are both disposed inside the housing (1), the controller (33) is electrically connected to the temperature sensor (34), the heating device (31) and the cooling device (32), respectively, and the controller (33) is configured to receive the temperature measured by the temperature sensor (34) and control the heating device (31) and the cooling device (32) according to the temperature.
4. A flow cytometer sampler as in claim 3 wherein said temperature sensor (34) is provided in plurality and said temperature sensor (34) is uniformly fixed to the tube rack (2).
5. The flow cytometer sampler of claim 1, wherein the stop pad (22) is made of rubber.
6. The flow cytometer sampler of claim 1, wherein a sealing gasket (12) is fixed at the top end of the housing (1), and a sealing groove (111) corresponding to and matching with the sealing gasket (12) is arranged at the inner side of the upper cover (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320759997.3U CN219978087U (en) | 2023-04-07 | 2023-04-07 | Flow cytometer sampler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320759997.3U CN219978087U (en) | 2023-04-07 | 2023-04-07 | Flow cytometer sampler |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219978087U true CN219978087U (en) | 2023-11-07 |
Family
ID=88588608
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320759997.3U Active CN219978087U (en) | 2023-04-07 | 2023-04-07 | Flow cytometer sampler |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219978087U (en) |
-
2023
- 2023-04-07 CN CN202320759997.3U patent/CN219978087U/en active Active
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