CN219972272U - Constant temperature heater for nucleic acid detection - Google Patents
Constant temperature heater for nucleic acid detection Download PDFInfo
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- CN219972272U CN219972272U CN202321304925.6U CN202321304925U CN219972272U CN 219972272 U CN219972272 U CN 219972272U CN 202321304925 U CN202321304925 U CN 202321304925U CN 219972272 U CN219972272 U CN 219972272U
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- reaction tank
- nucleic acid
- circuit board
- control circuit
- acid detection
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- 238000001514 detection method Methods 0.000 title claims abstract description 28
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 27
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 27
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 27
- 238000010438 heat treatment Methods 0.000 claims abstract description 78
- 238000006243 chemical reaction Methods 0.000 claims abstract description 73
- 238000009413 insulation Methods 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 239000002657 fibrous material Substances 0.000 claims description 5
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 4
- 229910052782 aluminium Inorganic materials 0.000 claims description 4
- 239000011324 bead Substances 0.000 claims description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 239000010949 copper Substances 0.000 claims description 3
- 239000006261 foam material Substances 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 3
- 238000012546 transfer Methods 0.000 description 6
- 238000009529 body temperature measurement Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 229920000139 polyethylene terephthalate Polymers 0.000 description 3
- 239000005020 polyethylene terephthalate Substances 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- -1 Polyethylene terephthalate Polymers 0.000 description 1
- 239000004642 Polyimide Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011901 isothermal amplification Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920001721 polyimide Polymers 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a constant temperature heater for nucleic acid detection, comprising: the reaction tank comprises a control circuit board, a reaction tank body, a plurality of heat conducting plates, a plurality of heating elements, a temperature measuring element and a plurality of adjustable resistors; the control circuit board and the heat conducting plate are respectively arranged at two sides of the reaction tank body; the reaction tank body comprises a plurality of reaction tanks which are arranged at intervals along the length direction of the reaction tank body; the reaction tank comprises two slots, the first slot is positioned on the upper surface of the reaction tank body, the second slot is positioned on the side surface of the reaction tank body, and a heat-conducting plate is arranged at the second slot; one side of each heat conducting plate is provided with a heating element; each heating element is respectively connected with an adjustable resistor; each adjustable resistor is connected with a control circuit board, and the control circuit board is powered by a power supply; the temperature measuring element is arranged on one heating element; the temperature measuring element is connected with the control circuit board. The constant temperature heater can realize multi-channel constant temperature control while taking portability and low cost into consideration.
Description
Technical Field
The utility model belongs to the technical field of nucleic acid detection, and particularly relates to a constant temperature heater for nucleic acid detection.
Background
Compared with the traditional variable-temperature amplification, the heating module for constant-temperature amplification can obviously reduce the difficulty between the balance temperature control precision and the variable-temperature speed. This makes the temperature-changing heating module with large size, high cost and large power consumption unsuitable for nucleic acid detection equipment based on isothermal amplification technology.
A multi-channel (slot/well) isothermal heating module is used when the isothermal heating requirements of multiple nucleic acid detection reactions are met simultaneously. The multi-channel constant temperature heating is divided into a single-temperature-zone heating module and a multi-temperature-zone heating module. The single-temperature-zone heating module needs to ensure the temperature consistency among different channels, and the multi-temperature-zone heating module needs to establish stable temperature difference among different temperature zones.
The single-temperature-zone heating module greatly reduces the size and cost of the traditional constant-temperature heater, but cannot realize the constant-temperature control of multiple temperature zones. The multi-temperature zone heating module can realize multi-temperature zone heating, but each channel uses an independent temperature control element, so that the cost is greatly increased. Therefore, there is a problem that it is not possible to achieve the low cost and the constant temperature control in a plurality of temperature zones in the constant temperature heating for a plurality of nucleic acid detection reactions.
Disclosure of Invention
In order to overcome the problems in the related art, the embodiment of the utility model provides a constant temperature heater for nucleic acid detection, which can realize the constant temperature control of multiple temperature areas while considering low cost.
The utility model is realized by the following technical scheme:
in a first aspect, embodiments of the present utility model provide a isothermal heater for nucleic acid detection, comprising: the reaction tank comprises a control circuit board, a reaction tank body, a plurality of heat conducting plates, a plurality of heating elements, a temperature measuring element and a plurality of adjustable resistors;
the control circuit board and the heat conducting plate are respectively arranged at two sides of the reaction tank body;
the reaction tank body comprises a plurality of reaction tanks which are arranged at intervals along the length direction of the reaction tank body; the reaction tank comprises a first notch and a second notch, the first notch is positioned on the upper surface of the reaction tank body, and the second notch is positioned on the side surface of the reaction tank body; a heat conducting plate is correspondingly arranged at each second notch;
one side of each heat conducting plate far away from the reaction tank body is provided with a heating element;
each heating element is respectively connected with an adjustable resistor; each adjustable resistor is respectively connected with a control circuit board, and the control circuit board is powered by a power supply;
the temperature measuring element is arranged at one side of any heating element far away from the heat conducting plate; the temperature measuring element is connected with the control circuit board.
In one embodiment, the device further comprises a heat insulation structure and a plurality of limiting elements;
the heat insulation structure is positioned on one side of the heating element far away from the heat conducting plate;
and the limiting element is positioned in each reaction groove and used for pressing the sample to be tested on the heat-conducting plate.
In one embodiment, the limiting element includes at least one of a spring plate and a spring plunger.
In one embodiment, the limiting element includes a spring plate; the lateral wall of reaction tank is provided with first recess, and the bottom side of reaction tank is provided with the second recess, and first recess and second recess are used for fixed shell fragment.
In one embodiment, the thickness of the spring plate ranges from 0.2 mm to 0.4mm; the width of the reaction groove is larger than the thickness of the elastic sheet.
In one embodiment, the input voltage range of the control circuit board is 3-12V, and the input power range is 4.5-36W.
In one embodiment, the temperature measuring element comprises an NTC bead thermistor or an NTC patch thermistor.
In one embodiment, the heating element comprises a PI heating film, a PET heating film, or a ceramic heating plate.
In one embodiment, the material of the heat conducting plate is pure copper or pure aluminum, and the thickness of the heat conducting plate ranges from 0.5 mm to 2.5mm.
In one embodiment, the insulating structure comprises a fibrous material or a foam material.
Compared with the prior art, the embodiment of the utility model has the beneficial effects that:
according to the embodiment of the utility model, the semi-independent temperature control of the temperature areas is finished by matching the temperature measuring elements and the heating elements with the independent adjustable resistors, the temperature measuring elements are used for the heating channels, so that gradient heating is realized, low cost is realized, and meanwhile, the constant temperature control of the temperature areas is realized.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments or the description of the prior art will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram showing the structure of a constant temperature heater for nucleic acid detection according to an embodiment of the present utility model;
FIG. 2 is an exploded view showing the structure of a constant temperature heater for nucleic acid detection according to an embodiment of the present utility model;
FIG. 3 is a schematic diagram of an electrical connection relationship according to an embodiment of the present utility model;
FIG. 4 is a schematic cross-sectional view of a constant temperature heater for nucleic acid detection according to an embodiment of the present utility model.
Detailed Description
In the following description, for purposes of explanation and not limitation, specific details are set forth such as the particular system architecture, techniques, etc., in order to provide a thorough understanding of the embodiments of the present utility model. It will be apparent, however, to one skilled in the art that the present utility model may be practiced in other embodiments that depart from these specific details. In other instances, detailed descriptions of well-known systems, devices, circuits, and methods are omitted so as not to obscure the description of the present utility model with unnecessary detail.
It should be understood that the terms "comprises" and/or "comprising," when used in this specification and the appended claims, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof.
It should also be understood that the term "and/or" as used in the present specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations.
Furthermore, the terms "first," "second," "third," and the like in the description of the present specification and in the appended claims, are used for distinguishing between descriptions and not necessarily for indicating or implying a relative importance.
Reference in the specification to "one embodiment" or "some embodiments" or the like means that a particular feature, structure, or characteristic described in connection with the embodiment is included in one or more embodiments of the utility model. Thus, appearances of the phrases "in one embodiment," "in some embodiments," "in other embodiments," and the like in the specification are not necessarily all referring to the same embodiment, but mean "one or more but not all embodiments" unless expressly specified otherwise. The terms "comprising," "including," "having," and variations thereof mean "including but not limited to," unless expressly specified otherwise.
In combination with the technical problems of the background technology, the utility model can heat the detection sample in the shape of the card, and aims to control the manufacturing cost and the whole size by using low-cost small-size materials, simultaneously, based on a group of temperature measuring and heating elements, the temperature control precision is improved by matching with an adjustable resistor convenient to debug, and the semi-independent temperature control of a plurality of temperature areas is completed, so that gradient heating is realized.
The utility model is described in further detail below with reference to the drawings and the detailed description.
Fig. 1 is a structural diagram of a constant temperature heater for nucleic acid detection according to an embodiment of the present utility model, and fig. 2 is an exploded structural diagram of a constant temperature heater for nucleic acid detection according to an embodiment of the present utility model, and referring to fig. 1 and 2, the constant temperature heater for nucleic acid detection includes: the reaction tank comprises a control circuit board 100, a reaction tank body 200, a plurality of heat conducting plates 300, a plurality of heating elements 400, a temperature measuring element 500 and a plurality of adjustable resistors 600.
The control circuit board 100 and the heat conductive plate 300 are disposed at both sides of the reaction tank body 200, respectively. The reaction tank 200 includes a plurality of reaction tanks 201, and the plurality of reaction tanks 201 are arranged at intervals along the length direction of the reaction tank 200; the reaction tank 201 includes a first notch 2011 and a second notch 2012, wherein the first notch 2011 is located on the upper surface of the reaction tank body 200, and the second notch 2012 is located on the side surface of the reaction tank body 200; one heat conductive plate 300 is disposed at each second notch 2012. One heating element 400 is provided at a side of each of the heat conductive plates 300 remote from the reaction tank 200. Each heating element 400 is connected to a respective adjustable resistor 600; each adjustable resistor 600 is connected to the control circuit board 100, and the control circuit board 100 is powered by a power source. The temperature measuring element 500 is disposed at a side of any one of the heating elements 400 remote from the heat conductive plate 300; the temperature measuring element 500 is connected to the control circuit board 100.
Illustratively, each heating element 400 is connected to an adjustable resistor 600, and the adjustable resistor 600 may be disposed on a side of the control circuit board 100 away from the reaction tank 200, and each adjustable resistor 600 is connected to the control circuit board 100, where the control circuit board 100 is powered by a power source. The temperature measuring element 500 is disposed on any one of the heating elements 400 far from the heat conducting plate 300, the temperature measuring element 500 is connected with the control circuit board 100, and temperature data is transmitted to the control circuit board 100, and the connection relationship can be realized through wiring, which is not shown in fig. 1 and 2, and the specific relationship is schematically shown in fig. 3.
For example, the reaction tank body 200 may include a plurality of reaction tanks 201, and the number of the reaction tanks 201 may be three, and then three heating elements are connected in parallel on the same power supply to realize simultaneous heating to form three temperature zones. Before the test sample is heated by the constant temperature heater, the set working temperatures of the channels of the three reaction tanks 201 are determined to be 59 ℃, 62 ℃ and 65 ℃, the actual working temperatures of the temperature areas of the three channels are tested in real time to be 62 ℃, 61 ℃ and 63 ℃, and the adjustable resistances 600 of the three temperature areas are respectively adjusted until the actual working temperatures are consistent with the set working temperatures, and at the moment, the calibration of the constant temperature heater is completed, so that the reaction tank 201 without the test sample is placed under the set working temperature requirement.
The test sample is placed in the reaction tank 201 for heating, and the three temperature areas are connected in parallel on the same power supply for realizing simultaneous heating, and the distribution condition of the temperature fields of the three temperature areas is fixed, so that the temperature consistency is convenient to control. The temperature measuring element 500 is arranged on any heating element 400, and the temperature of any point in the temperature field is measured, so that the temperature of any point in the three temperature areas can be obtained through the temperature distribution condition, and therefore, one temperature measuring element 500 can be used for realizing the temperature measurement of the three temperature areas, and the test sample is ensured to be heated at the set working temperature in the heating process. And, when the actual operating temperatures of the three temperature areas deviate from the set operating temperature, the adjustable resistor 600 is adjusted according to the temperature measured by the temperature measuring element 500 until the actual operating temperature is consistent with the set operating temperature.
The temperature measuring element 500 may be a temperature measuring resistor, the heating element 400 provided with the temperature measuring resistor may realize direct temperature measurement, and the heating element 400 without the temperature measuring resistor may perform indirect temperature measurement.
Semi-independent temperature control of a plurality of temperature areas is completed through the temperature measuring element 500 and the heating elements 400 and the independent adjustable resistors 600, the temperature control elements for measuring, heating and adjusting are used for a plurality of heating channels, gradient heating is realized, portability is realized, low cost is achieved, multi-channel constant temperature control is realized, and heating efficiency is improved.
Illustratively, the reaction tank 200 is a material having high heat insulation, and the heating element 400 is disposed at a side of the reaction tank 200, and heat transfer between each reaction tank 201 is small. The reaction tank 200 may be made of plastic.
By way of example, the temperature of the plurality of reaction tanks 201 can be adjusted to the same temperature by using one temperature measuring element 500 and a plurality of heating elements 400 in combination with a plurality of independent adjustable resistors 600, so that the heating of the multi-channel single temperature zone can be realized.
In one embodiment, referring to fig. 2 and 4, the isothermal heater for nucleic acid detection further comprises an insulating structure 700 and a plurality of spacing elements 800. And a thermal insulation structure located at a side of the heating element 400 remote from the heat conductive plate 300. And a limiting element, which is positioned in each reaction tank 201 and is used for pressing the sample to be tested on the heat conducting plate 300.
The heat insulation structure may be fastened to the side of the reaction tank 200 by a fastening structure, or may be adhered to the side of the reaction tank 200 by adhesion or the like.
In one embodiment, the limiting element includes at least one of a spring plate and a spring plunger.
In one embodiment, the limiting element includes a spring plate; the side wall of the reaction tank 201 is provided with a first groove 202, the bottom side of the reaction tank 201 is provided with a second groove 203, and the first groove 202 and the second groove 203 are used for fixing the elastic sheet.
Wherein, the thickness range of the spring plate is 0.2-0.4 mm; the width of the reaction tank 201 is larger than the thickness of the spring plate. If the limiting element is a spring plunger, the load range of the spring plunger is 2-15N.
For example, the elastic sheet is arranged in the direction perpendicular to the heat transfer surface to fix the card to be heated, which results in poor adhesion of the heat transfer surface and further reduces heat transfer efficiency. The limiting element comprising the elastic sheet is arranged in the direction parallel to the heat conducting plate 300 and the heating element 400, the thickness of the elastic sheet is limited, and the limiting element is only contacted with a card to be heated (a sample to be detected), so that the problem that the heat conducting plate 300 is directly or indirectly applied with excessive pressure, so that the heat transfer surface is not tightly attached, and the heat transfer efficiency is reduced is avoided.
Also, limiting the load of the spring plunger can avoid applying excessive pressure directly or indirectly to the heat-conducting plate 300.
In one embodiment, the input voltage range of the control circuit board 100 is 3-12V and the input power range is 4.5-36W.
By way of example, the provision of a circuit board may be implemented not only by battery power, but also by using a USB cable external power supply. When the USB line is used for supplying power, the problem of insufficient power of the battery due to higher heat resistance of the heating tank or the reaction tank can be solved, the heating efficiency is improved, the power supply provides enough power, and the temperature control precision and consistency are improved.
The reaction tank 200 is provided with a column 204, and the column 204 is used for forming a certain space between the reaction tank 200 and the control circuit board 100, so as to prevent the extrusion of circuit components on the control circuit board 100.
In one embodiment, the temperature measuring element 500 comprises an NTC (Negative Temperature Coefficient ) bead thermistor or an NTC patch thermistor. The NTC bead thermistor may be 0402 or 0603 in size.
In one embodiment, the heating element 400 comprises a PI (Polyimide) heating film, a PET (Polyethylene terephthalate, polyester) heating film, or a ceramic heating sheet. The maximum operating current range of the heating element 400 may be 0.3-1.2A. The heating temperature range of the heating element 400 may be: room temperature+ (5 ℃ C. To 80 ℃ C.).
Illustratively, the maximum resistance of the adjustable resistor 600 may be 5% to 200% of the heating element 400.
In one embodiment, the material of the heat conducting plate 300 is pure copper or pure aluminum, and the thickness of the heat conducting plate 300 ranges from 0.5 mm to 2.5mm.
In one embodiment, the insulating structure comprises a fibrous material or a foam material. Wherein the fibrous material may be selected from glass fibrous materials.
Illustratively, each reaction tank 201 of the three temperature zones may have a width of 3.6mm, a length of 12.2mm, a depth of 22mm, a spacing element may be a spring sheet with a thickness of 0.3mm, the heat conducting plate 300 may be pure aluminum with a thickness of 1.5mm, the heating elements 400 are PI heating films, and the manufacturing cost and the overall size of the constant temperature heater may be controlled by small-sized elements.
Therefore, the utility model can control the manufacturing cost and the whole size by using low-cost small-size materials, and can improve the temperature control precision by using the resistor convenient to debug. Based on a group of temperature measurement and heating elements, a plurality of independent resistors are matched to complete semi-independent temperature control of a plurality of temperature areas, so that gradient heating is realized or temperature consistency is ensured.
The above embodiments are only for illustrating the technical solution of the present utility model, and not for limiting the same; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present utility model, and are intended to be included in the scope of the present utility model.
Claims (10)
1. A constant temperature heater for nucleic acid detection, comprising: the reaction tank comprises a control circuit board, a reaction tank body, a plurality of heat conducting plates, a plurality of heating elements, a temperature measuring element and a plurality of adjustable resistors;
the control circuit board and the heat conducting plate are respectively arranged at two sides of the reaction tank body;
the reaction tank body comprises a plurality of reaction tanks, and the reaction tanks are arranged at intervals along the length direction of the reaction tank body; the reaction tank comprises a first notch and a second notch, the first notch is positioned on the upper surface of the reaction tank body, and the second notch is positioned on the side surface of the reaction tank body; a heat conducting plate is correspondingly arranged at each second notch;
one side of each heat conducting plate, which is far away from the reaction tank body, is provided with one heating element;
each heating element is respectively connected with one adjustable resistor; each adjustable resistor is connected with the control circuit board, and the control circuit board is powered by a power supply;
the temperature measuring element is arranged on one side of any one of the heating elements, which is far away from the heat conducting plate; the temperature measuring element is connected with the control circuit board.
2. The isothermal heater for nucleic acid detection according to claim 1, further comprising a thermal insulation structure and a plurality of spacing elements;
the heat insulation structure is positioned on one side of the heating element away from the heat conducting plate;
the limiting elements are positioned in each reaction groove and used for pressing the sample to be tested on the heat conducting plate.
3. The constant temperature heater for nucleic acid detection as claimed in claim 2, wherein the limiting member includes at least one of a spring plate and a spring plunger.
4. The constant temperature heater for nucleic acid detection as claimed in claim 2, wherein the limiting member comprises a spring sheet; the side wall of the reaction tank is provided with a first groove, the bottom side of the reaction tank is provided with a second groove, and the first groove and the second groove are used for fixing the elastic sheet.
5. The constant temperature heater for nucleic acid detection according to claim 4, wherein the thickness of the elastic sheet is in a range of 0.2 to 0.4mm; the width of the reaction groove is larger than the thickness of the elastic sheet.
6. The constant temperature heater for nucleic acid detection as claimed in claim 1, wherein the input voltage of the control circuit board ranges from 3 to 12V, and the input power of the control circuit board ranges from 4.5 to 36W.
7. A constant temperature heater for nucleic acid detection as claimed in claim 1, wherein the temperature sensing element comprises an NTC bead thermistor or an NTC patch thermistor.
8. The isothermal heater for nucleic acid detection according to claim 1 wherein the heating element comprises a PI heating film, a PET heating film, or a ceramic heating plate.
9. The constant temperature heater for nucleic acid detection as claimed in claim 1, wherein the material of the heat conducting plate is pure copper or pure aluminum, and the thickness of the heat conducting plate ranges from 0.5 to 2.5mm.
10. The isothermal heater for nucleic acid detection according to claim 2, wherein the insulating structure comprises a fibrous material or a foam material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321304925.6U CN219972272U (en) | 2023-05-25 | 2023-05-25 | Constant temperature heater for nucleic acid detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321304925.6U CN219972272U (en) | 2023-05-25 | 2023-05-25 | Constant temperature heater for nucleic acid detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219972272U true CN219972272U (en) | 2023-11-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321304925.6U Active CN219972272U (en) | 2023-05-25 | 2023-05-25 | Constant temperature heater for nucleic acid detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219972272U (en) |
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2023
- 2023-05-25 CN CN202321304925.6U patent/CN219972272U/en active Active
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