CN219951004U - Urokinase preparation is with vacuum suction filtration application of sample equipment - Google Patents
Urokinase preparation is with vacuum suction filtration application of sample equipment Download PDFInfo
- Publication number
- CN219951004U CN219951004U CN202321257048.1U CN202321257048U CN219951004U CN 219951004 U CN219951004 U CN 219951004U CN 202321257048 U CN202321257048 U CN 202321257048U CN 219951004 U CN219951004 U CN 219951004U
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- CN
- China
- Prior art keywords
- sample
- urokinase
- stirring tank
- tube
- pipe
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- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 title claims abstract description 29
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 title claims abstract description 29
- 229960005356 urokinase Drugs 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 238000000967 suction filtration Methods 0.000 title claims description 8
- 238000003756 stirring Methods 0.000 claims abstract description 39
- 238000007789 sealing Methods 0.000 claims abstract description 26
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000000605 extraction Methods 0.000 claims abstract description 9
- 238000003828 vacuum filtration Methods 0.000 claims abstract description 9
- 238000011068 loading method Methods 0.000 claims description 10
- 238000011049 filling Methods 0.000 claims description 7
- 230000000694 effects Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 17
- 238000002347 injection Methods 0.000 description 11
- 239000007924 injection Substances 0.000 description 11
- 239000012043 crude product Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 238000005070 sampling Methods 0.000 description 5
- 210000002700 urine Anatomy 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 239000002156 adsorbate Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005429 filling process Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012958 reprocessing Methods 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Abstract
The utility model discloses vacuum filtration sample adding equipment for urokinase preparation, which comprises a stirring tank and a sealing element, wherein the top end opening of the stirring tank is arranged, the sealing element is hermetically assembled at the top end of the stirring tank, the sealing element comprises a threaded cover and a sample adding device, the threaded cover is in threaded sealing connection with the opening end of the stirring tank, one side of the top surface of the threaded cover is vertically communicated and fixed with an extraction pipe, the extraction pipe is connected with a vacuum pump, the other side of the top surface of the threaded cover is vertically communicated and fixed with a feeding pipe orifice, a first valve is arranged on the feeding pipe orifice, the sample adding device is communicated and hermetically assembled at the top end of the feeding pipe orifice, and the sample adding device is used for adding saturated ammonium sulfate solution into the stirring tank. The utility model solves the problems that when the existing injector is used, a stirring device in a closed environment is required to be opened when the crude urokinase is prepared, so that the closed environment of the later-stage preparation of the crude urokinase is damaged, urokinase can overflow and scatter, and the preparation effect of the crude urokinase is poor.
Description
Technical Field
The utility model relates to the technical field of sample applicators, in particular to vacuum filtration sample applying equipment for urokinase preparation.
Background
Urokinase is a protease separated from urine of healthy human or obtained from tissue culture of human kidney, and is prepared by reprocessing crude urokinase, and the current preparation process of crude urokinase mainly comprises the following steps: fresh urine, impurity removal, urine treatment, absorption, adsorbate salting out, cleaning, washing, elution, sulfuric acid precipitation and vacuum suction filtration, wherein the last two steps are that purified saturated ammonium sulfate is added into the liquid collected in the previous step, the liquid is stirred uniformly by a stirring device and then is kept stand for 8 hours to precipitate the liquid, the precipitate liquid is subjected to vacuum suction filtration, the stirring device is started to be shoveled by a small iron shovel, and then urokinase crude products are collected, and the saturated ammonium sulfate solution is required to be added into the eluting liquid before the vacuum suction filtration is carried out on the existing urokinase to precipitate the urokinase crude products in the urine.
The existing urokinase crude product preparation is carried out in a closed environment, and most of the saturated ammonium sulfate solution is added by adopting a syringe, so that a stirring device in the closed environment is required to be opened when the syringe is used for preparing the urokinase crude product, the closed environment of the later-stage urokinase crude product preparation is damaged, and urokinase element overflows, so that the urokinase crude product preparation effect is poor.
Disclosure of Invention
The utility model aims to provide vacuum suction filtration sample adding equipment for urokinase preparation, and aims to solve the problems.
The utility model is realized in the following way:
the utility model provides a urokinase preparation is with vacuum suction filtration application of sample equipment, including agitator tank and sealing member, the open-top of agitator tank sets up, sealing member seals the top of assembly at the agitator tank, the sealing member includes screw cap and application of sample ware, wherein screw cap screw thread sealing connection is in the open end department of agitator tank, and the vertical intercommunication in top surface one side of screw cap is fixed with the extraction pipe, and be connected with the vacuum pump on the extraction pipe, the vertical intercommunication of the top surface opposite side of screw cap is fixed with the feeding mouth of pipe, and install first valve on the feeding mouth of pipe, application of sample ware intercommunication seals the equipment on feeding mouth of pipe's top, and the application of sample ware is used for filling saturated ammonium sulfate solution and gets into in the agitator tank.
Further, the sampler comprises a sampling tube, the top end opening of the sampling tube is provided with an interface, the bottom end of the sampling tube is fixedly connected with the interface, and the interface is communicated with the sealing assembly on the feeding tube orifice.
Further, the open end of the sampling tube is fixed with a top plate in a sealing way, and an air bag is arranged on the bottom end face of the top plate.
Further, an air inlet pipe penetrates through the top plate at the air inlet end of the air bag, and an air pump is arranged on the air inlet pipe.
Further, the outer circumferential surface bottom of the sampling tube is fixedly communicated with a feeding tube, and a second valve is arranged on the feeding tube.
Further, the internal thread has been seted up at the inner wall top of agitator tank, and screw cap and the inside internal thread screw assembly connection of agitator tank.
Further, the bottom of screw cap inserts the agitator tank and rotates and be connected with the (mixing) shaft, and the top of screw cap is vertical to be fixed with the motor, and the output of motor is connected on the top of (mixing) shaft.
Compared with the prior art, the utility model has the beneficial effects that: in use, the stirring tank is sealed by the threaded cover in the sealing piece, the feeding pipe orifice arranged on the threaded cover is used for being inserted and communicated with the sample adding device, the saturated ammonium sulfate solution is added into the stirring tank, the first valve arranged on the feeding pipe orifice is opened during adding, the saturated ammonium sulfate solution is added into the stirring tank, and then the first valve arranged on the feeding pipe orifice is closed, so that the stirring tank is sealed, and the filling process is carried out under the sealing condition, so that the sealing environment of the stirring tank is prevented from being damaged.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of the overall structure of the present utility model;
FIG. 2 is a schematic exploded view of the present utility model;
FIG. 3 is a schematic view of the seal in an exploded configuration in accordance with an embodiment of the present utility model;
fig. 4 is a schematic view showing a structure of the seal member in an exploded state in the embodiment of the present utility model.
In the figure: 1. a stirring tank; 11. an internal thread; 2. a seal; 21. a screw cap; 22. a motor; 23. a stirring shaft; 24. an extraction tube; 25. a vacuum pump; 26. a sample applicator; 261. a sample adding tube; 262. an interface; 263. a top plate; 264. an air bag; 265. an air inlet pipe; 266. an air pump; 267. a feeding tube; 268. a second valve; 27. a charging pipe orifice; 271. a first valve.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments. All other embodiments, based on the embodiments of the utility model, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the utility model. Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, based on the embodiments of the utility model, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the utility model.
Referring to fig. 1, 2, 3 and 4, a vacuum filtration sample loading device for urokinase preparation comprises a stirring tank 1 and a sealing element 2, wherein the top end opening of the stirring tank 1 is provided, the sealing element 2 is assembled at the top end of the stirring tank 1 in a sealing way, the sealing element 2 comprises a threaded cover 21 and a sample loading device 26, wherein the threaded cover 21 is connected at the opening end of the stirring tank 1 in a threaded way, one side of the top surface of the threaded cover 21 is vertically communicated and fixed with an extraction pipe 24, a vacuum pump 25 is connected on the extraction pipe 24, the other side of the top surface of the threaded cover 21 is vertically communicated and fixed with a feeding pipe orifice 27, a first valve 271 is arranged on the feeding pipe orifice 27, the sample loading device 26 is assembled at the top end of the feeding pipe orifice 27 in a communicating way, the sample loading device 26 is used for loading saturated ammonium sulfate solution into the stirring tank 1, the top of the inner wall of the stirring tank 1 is provided with an internal thread 11, the screw cap 21 is in screw assembly connection with the internal screw thread 11 in the stirring tank 1, the bottom end of the screw cap 21 is inserted into the stirring tank 1, the stirring shaft 23 is rotationally connected, the top end of the screw cap 21 is vertically fixed with the motor 22, the output end of the motor 22 is connected with the top end of the stirring shaft 23, the motor 22 drives the stirring shaft 23 to rotate, the saturated ammonium sulfate solution in the stirring tank 1 is stirred and dissolved, the stirring tank 1 is sealed through the screw cap 21 in the sealing piece 2 in use, the charging pipe orifice 27 arranged on the screw cap 21 is used for being inserted and communicated with the sampler 26, the saturated ammonium sulfate solution is added into the stirring tank 1, the first valve 271 arranged on the charging pipe orifice 27 is opened during charging, the saturated ammonium sulfate solution is added into the stirring tank 1, then the first valve 271 arranged on the charging pipe orifice 27 is closed, the stirring tank 1 is sealed, and thus the charging process is carried out under sealed conditions, avoiding damaging the closed environment of the stirring tank 1.
Referring to fig. 3 and 4, the sample injector 26 includes a sample injection tube 261, a top end opening of the sample injection tube 261 is disposed, a bottom end of the sample injection tube 261 is fixedly connected with an interface 262, the interface 262 is assembled on a sample injection tube 27 in a sealing manner, an opening end of the sample injection tube 261 is fixedly connected with a top plate 263, an air bag 264 is disposed on a bottom end surface of the top plate 263, an air inlet end of the air bag 264 penetrates through the top plate 263 and is fixedly connected with an air inlet tube 265, an air pump 266 is mounted on the air inlet tube 265, a filling tube 267 is fixedly connected with an outer circumferential bottom of the sample injection tube 261, a second valve 268 is mounted on the filling tube 267, in use, the interface at the bottom end of the sample injection tube 261 is assembled on the sample injection tube 27 of the screw cap 21 in a sealing manner, then the second valve 268 is opened, a proper amount of saturated ammonium sulfate solution is introduced into the sample injection tube 261 through the filling tube 267, the first valve 271 is opened, an air pump 266 on the air bag 264 is started to enter air, the saturated ammonium sulfate solution in the sample injection tube 261 is pushed under expansion, and the saturated ammonium sulfate solution in the sample injection tube 261 enters the air bag, and the first valve 271 is completely filled after the filling tube 27 is completely filled.
Working principle: in use, the stirring tank 1 is sealed by the threaded cover 21 in the sealing piece 2, the feeding pipe orifice 27 arranged on the threaded cover 21 is used for being spliced and communicated with the sample adding device 26, the interface at the bottom end of the sample adding pipe 261 is firstly assembled at the feeding pipe orifice 27 of the threaded cover 21 in a sealing way, then the second valve 268 on the feeding pipe 267 is opened, saturated ammonium sulfate solution with proper addition amount is introduced into the middle sample adding pipe 261 through the feeding pipe 267, the second valve 268 on the feeding pipe 267 is closed, the first valve 271 arranged on the feeding pipe orifice 27 is opened, the air pump 266 on the air bag 264 is started to input air, the saturated ammonium sulfate solution in the sample adding pipe 261 is pushed under the expansion of the air bag 264 to be filled into the feeding pipe orifice 27, and after filling is finished, the first valve 271 arranged on the feeding pipe orifice 27 is closed.
The above description is only of the preferred embodiments of the present utility model and is not intended to limit the present utility model, and various modifications and variations may be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (7)
1. Vacuum suction filtration sample loading equipment for urokinase preparation, characterized by, including agitator tank (1) and sealing member (2), the open-top of agitator tank (1) sets up, sealing member (2) seal assembly is on the top of agitator tank (1), sealing member (2) are including screw thread lid (21) and application of sample ware (26), wherein screw thread lid (21) screw thread sealing connection is in the open end department of agitator tank (1), and the vertical intercommunication in top surface one side of screw thread lid (21) is fixed with extraction pipe (24), and is connected with vacuum pump (25) on extraction pipe (24), the vertical intercommunication in top surface opposite side of screw thread lid (21) is fixed with feeding mouth of pipe (27), and installs first valve (271) on feeding mouth of pipe (27), application of sample ware (26) intercommunication seal assembly is on the top of feeding mouth of pipe (27), and application of sample ware (26) are arranged in filling saturated ammonium sulfate solution entering agitator tank (1).
2. The vacuum filtration and sample addition device for urokinase preparation according to claim 1, wherein the sample addition device (26) comprises a sample addition tube (261), the top end opening of the sample addition tube (261) is provided, an interface (262) is fixedly communicated with the bottom end of the sample addition tube (261), and the interface (262) is hermetically assembled on a feed pipe orifice (27) in a communicating manner.
3. The vacuum filtration and sample loading device for urokinase preparation according to claim 2, wherein the top plate (263) is sealed and fixed at the opening end of the sample loading tube (261), and an air bag (264) is arranged on the bottom end surface of the top plate (263).
4. A vacuum filtration and sample loading device for urokinase preparation according to claim 3, wherein the air inlet end of the air bag (264) penetrates through the top plate (263) and is fixedly provided with an air inlet pipe (265), and the air inlet pipe (265) is provided with an air pump (266).
5. The vacuum filtration and sample addition device for urokinase preparation according to claim 4, wherein a feeding tube (267) is fixedly connected to the bottom of the outer circumferential surface of the sample addition tube (261), and a second valve (268) is installed on the feeding tube (267).
6. The vacuum filtration and sample loading device for urokinase preparation according to claim 1, wherein the inner wall top of the stirring tank (1) is provided with an internal thread (11), and the threaded cover (21) is in threaded assembly connection with the internal thread (11) inside the stirring tank (1).
7. The vacuum filtration and sample addition device for urokinase preparation according to claim 6, wherein the bottom end of the screw cap (21) is inserted into the stirring tank (1) and is rotatably connected with a stirring shaft (23), the top end of the screw cap (21) is vertically fixed with a motor (22), and the output end of the motor (22) is connected to the top end of the stirring shaft (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321257048.1U CN219951004U (en) | 2023-05-23 | 2023-05-23 | Urokinase preparation is with vacuum suction filtration application of sample equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321257048.1U CN219951004U (en) | 2023-05-23 | 2023-05-23 | Urokinase preparation is with vacuum suction filtration application of sample equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219951004U true CN219951004U (en) | 2023-11-03 |
Family
ID=88545444
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321257048.1U Active CN219951004U (en) | 2023-05-23 | 2023-05-23 | Urokinase preparation is with vacuum suction filtration application of sample equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219951004U (en) |
-
2023
- 2023-05-23 CN CN202321257048.1U patent/CN219951004U/en active Active
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| GR01 | Patent grant |