CN219950992U - Exosome detection device for tumor detection and molecular typing - Google Patents
Exosome detection device for tumor detection and molecular typing Download PDFInfo
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- CN219950992U CN219950992U CN202321314230.6U CN202321314230U CN219950992U CN 219950992 U CN219950992 U CN 219950992U CN 202321314230 U CN202321314230 U CN 202321314230U CN 219950992 U CN219950992 U CN 219950992U
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- exosome
- stirring
- modulation
- shaped
- fixedly connected
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 63
- 238000001514 detection method Methods 0.000 title claims abstract description 30
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 15
- 238000003756 stirring Methods 0.000 claims abstract description 92
- 238000007790 scraping Methods 0.000 claims abstract description 26
- 239000000463 material Substances 0.000 claims abstract description 12
- 238000013016 damping Methods 0.000 claims description 7
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 238000007599 discharging Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- JGLMVXWAHNTPRF-CMDGGOBGSA-N CCN1N=C(C)C=C1C(=O)NC1=NC2=CC(=CC(OC)=C2N1C\C=C\CN1C(NC(=O)C2=CC(C)=NN2CC)=NC2=CC(=CC(OCCCN3CCOCC3)=C12)C(N)=O)C(N)=O Chemical compound CCN1N=C(C)C=C1C(=O)NC1=NC2=CC(=CC(OC)=C2N1C\C=C\CN1C(NC(=O)C2=CC(C)=NN2CC)=NC2=CC(=CC(OCCCN3CCOCC3)=C12)C(N)=O)C(N)=O JGLMVXWAHNTPRF-CMDGGOBGSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003419 tautomerization reaction Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to the technical field of medical auxiliary instruments, and discloses an exosome detection device for tumor detection and molecular parting, which solves the problem that the existing exosome detection device is inconvenient to mix and stir samples, and comprises an exosome pre-modulation tank, wherein a discharge port is fixedly arranged at the bottom end of the exosome pre-modulation tank, a modulation driving assembly, a material dripping feeding port and a plurality of electric telescopic rods are fixedly arranged at the top end of the exosome pre-modulation tank, an exosome mixing and stirring assembly fixedly connected with the modulation driving assembly is movably arranged in the exosome pre-modulation tank, and a scraping ring which is adhered to the inner side wall of the exosome pre-modulation tank and fixedly connected with the electric telescopic rods is arranged at the side edge of the interior of the exosome pre-modulation tank; through this exosome detection device can realize modulating the stirring to the sample, improves the degree of consistency of stirring, and then improves the degree of accuracy that detects, avoids causing the misdiagnosis.
Description
Technical Field
The utility model belongs to the technical field of medical auxiliary instruments, and particularly relates to an exosome detection device for tumor detection and molecular typing.
Background
Tumor diagnosis and tumor prediction are usually performed by detecting whether the RNA content of exosomes is normal or not, and an ultraviolet absorption method is a mode of RNA detection, and the principle is that purine and pyrimidine bases in the molecular structure of nucleic acid, nucleotide and derivatives thereof have a conjugated double-bond system, can strongly absorb ultraviolet light with the wavelength of 250-280nm, the maximum ultraviolet absorption value of the nucleic acid is at 260nm, the content of nucleic acid substances can be detected from the change of the ultraviolet absorption value according to the Lambert-Beer law, the tautomerism of the purine and pyrimidine bases in different PH solutions is different, and the molar extinction coefficients of the purine and pyrimidine bases are also different.
In the operation process of sample treatment, 0.2-0.25g of crude RNA is required to be weighed, 2ml of 0.2% NaOH solution and 1ml of H2O solution are added to be mixed into paste, 40-50ml of H2O is added to be dissolved, pH is regulated to 7.0, and then 100ml of constant volume is obtained.
Disclosure of Invention
Aiming at the situation, in order to overcome the defects of the prior art, the utility model provides the exosome detection device for tumor detection and molecular typing, which effectively solves the problem that the existing exosome detection device is inconvenient to mix and stir samples.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides an exosome detection device for tumour detects and molecular typing, includes exosome pre-modulation jar, exosome pre-modulation jar's bottom mounting is provided with the bin outlet, and exosome pre-modulation jar's top is fixed to be provided with modulation drive assembly, material feed inlet and a plurality of electric telescopic handle, and exosome pre-modulation jar's inside activity is provided with the exosome mixing and stirring assembly with modulation drive assembly fixed connection, and exosome pre-modulation jar inside side is provided with and pastes the scraping ring with exosome pre-modulation jar inside wall subsides and with electric telescopic handle fixed connection.
Preferably, the modulation drive assembly comprises a frame type support frame, a drive motor, a main drive shaft, a first bevel gear, a rotating shaft, a second bevel gear, a stirring central shaft, a sleeve and a third bevel gear, wherein the frame type support frame is fixedly connected to the top end of the external secretion body pre-modulation tank, the drive motor is fixedly connected to the middle position of one side of the frame type support frame, the main drive shaft is fixedly connected to the output shaft of the drive motor, the first bevel gear is fixedly connected to one end of the main drive shaft, the second bevel gear is connected to the top end of the inside of the frame type support frame through the rotating shaft, the rotating shaft and the second bevel gear are in a fixed connection structure, the stirring central shaft is fixedly connected to the middle position of the bottom end of the second bevel gear, the sleeve is inserted into the frame type support frame and sleeved on the stirring central shaft, and the third bevel gear is fixedly connected to the top end of the sleeve and sleeved on the stirring central shaft.
Preferably, the main driving shaft, the rotating shaft, the sleeve and the frame-type supporting frame are movably connected through a first bearing, and the stirring central shaft is movably connected with the sleeve and a third bevel gear through a plurality of second bearings.
Preferably, the exosome mixing stirring assembly comprises a plurality of first stirring paddles, a plurality of L-shaped stirring arms and a plurality of second stirring paddles, wherein the first stirring paddles are fixedly connected to the side edge of the stirring central shaft, the L-shaped stirring arms are fixedly connected to the outer side edge of the sleeve, and the second stirring paddles are fixedly connected to the inner side edge of the L-shaped stirring arms and are alternately distributed with the first stirring paddles.
Preferably, the outside limit activity of L type stirring arm is provided with the bar scraper blade, through damping axle swing joint between bar scraper blade and the L type stirring arm, and the bar scraper blade is kept away from the fixed rubber strip that is provided with of one side of L type stirring arm.
Compared with the prior art, the utility model has the beneficial effects that:
(1) In operation, through setting the modulation driving assembly consisting of the frame-type supporting frame, the driving motor, the main driving shaft, the first bevel gear, the rotating shaft, the second bevel gear, the stirring central shaft, the sleeve and the third bevel gear and the exosome mixing and stirring assembly consisting of a plurality of first stirring paddles, a plurality of L-shaped stirring arms and a plurality of second stirring paddles, the samples can be uniformly mixed and stirred, so that the detection accuracy is improved, and misdiagnosis is avoided;
(2) The material scraping operation can be carried out on the inner side wall of the exosome pre-modulation tank by the aid of the electric telescopic rod, the scraping ring, the strip-shaped scraping plate, the damping shaft and the rubber scraping strip, so that the mixing uniformity is prevented from being influenced by the fact that a sample is adhered to the inner side wall of the exosome pre-modulation tank, and meanwhile the discharging effect can be improved.
Drawings
The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the embodiments of the utility model, serve to explain the utility model.
In the drawings:
FIG. 1 is a schematic diagram of an exosome detection device for tumor detection and molecular typing according to the present utility model;
FIG. 2 is a schematic diagram of a connection structure of the modulation driving assembly and the exosome mixing and stirring assembly according to the present utility model;
FIG. 3 is a schematic diagram of a modulation driving assembly according to the present utility model;
FIG. 4 is a schematic diagram of an exosome mixing and stirring assembly according to one embodiment of the present utility model;
FIG. 5 is a second schematic view of an exosome mixing and stirring assembly according to the present utility model;
in the figure: 1. an exosome pre-modulation tank; 2. a discharge port; 3. a modulation drive assembly; 4. a dropping material inlet; 5. an electric telescopic rod; 6. an exosome mixing and stirring assembly; 7. a scraping ring; 8. a frame-type support frame; 9. a driving motor; 10. a main drive shaft; 11. a first helical gear; 12. a rotating shaft; 13. a helical gear II; 14. stirring a central shaft; 15. a sleeve; 16. a helical gear III; 17. a first bearing; 18. a second bearing; 19. a first stirring paddle; 20. an L-shaped stirring arm; 21. a second stirring paddle; 22. a strip-shaped scraping plate; 23. a damping shaft; 24. rubber scraping strips.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model; all other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The utility model provides an exosome detection device for tumor detection and molecular parting, which is shown in fig. 1 and 2, and comprises an exosome pre-modulation tank 1, wherein a discharge port 2 is fixedly arranged at the bottom end of the exosome pre-modulation tank 1, a modulation driving assembly 3, a material dripping feeding port 4 and a plurality of electric telescopic rods 5 are fixedly arranged at the top end of the exosome pre-modulation tank 1, an exosome mixing and stirring assembly 6 fixedly connected with the modulation driving assembly 3 is movably arranged in the exosome pre-modulation tank 1, and a scraping ring 7 which is adhered to the inner side wall of the exosome pre-modulation tank 1 and fixedly connected with the electric telescopic rods 5 is arranged at the side edge in the exosome pre-modulation tank 1;
as shown in fig. 1 to 5, the modulation driving assembly 3 is composed of a frame-shaped supporting frame 8, a driving motor 9, a main driving shaft 10, a first bevel gear 11, a rotating shaft 12, a second bevel gear 13, a stirring central shaft 14, a sleeve 15 and a third bevel gear 16, wherein the frame-shaped supporting frame 8 is fixedly connected with the top end of the external secretion body pre-modulation tank 1, the driving motor 9 is fixedly connected with the middle position of one side of the frame-shaped supporting frame 8, the main driving shaft 10 is fixedly connected with the output shaft of the driving motor 9, the first bevel gear 11 is fixedly connected with one end of the main driving shaft 10, the second bevel gear 13 is connected with the top end inside the frame-shaped supporting frame 8 through the rotating shaft 12 and the second bevel gear 13 in a fixed connection structure, the stirring central shaft 14 is fixedly connected with the middle position of the bottom end of the second bevel gear 13, the sleeve 15 is inserted into the frame-shaped supporting frame 8 and sleeved on the stirring central shaft 14, the third helical gear 16 is fixedly connected to the top end of the sleeve 15 and sleeved on the stirring central shaft 14, the main driving shaft 10, the rotating shaft 12 and the sleeve 15 are movably connected with the frame type supporting frame 8 through a first bearing 17, the stirring central shaft 14 is movably connected with the sleeve 15 and the third helical gear 16 through a plurality of second bearings 18, the exosome mixing stirring assembly 6 consists of a plurality of first stirring paddles 19, a plurality of L-shaped stirring arms 20 and a plurality of second stirring paddles 21, the first stirring paddles 19 are fixedly connected to the side edge of the stirring central shaft 14, the L-shaped stirring arms 20 are fixedly connected to the outer side edge of the sleeve 15, the second stirring paddles 21 are fixedly connected to the inner side edge of the L-shaped stirring arms 20 and are alternately distributed with the first stirring paddles 19, the outer side edge of the L-shaped stirring arms 20 is movably provided with strip-shaped scraping plates 22, the strip-shaped scraping plates 22 are movably connected with the L-shaped stirring arms 20 through damping shafts 23, a rubber scraping strip 24 is fixedly arranged on one side of the strip-shaped scraping plate 22 away from the L-shaped stirring arm 20;
when in use, crude RNA and solutions are poured into the external secretion body pre-regulating tank 1 from the dripping material feeding hole 4, the driving motor 9 is started, the driving motor 9 drives the main driving shaft 10 to rotate, the main driving shaft 10 drives the bevel gear I11 to rotate, the bevel gear I11 synchronously drives the bevel gear II 13 and the bevel gear III 16 to rotate in opposite directions, the bevel gear II 13 drives the stirring central shaft 14 to rotate, the bevel gear III 16 drives the sleeve 15 to rotate, and the stirring central shaft 14 is opposite to the sleeve 15 in rotation direction, and the stirring central shaft 14 is movably connected with the sleeve 15 and the bevel gear III 16 through a plurality of bearings II 18, so that the movement resistance can be reduced, the activity is improved, the stirring central shaft 14 drives the first stirring paddle 19 to rotate, the sleeve 15 drives the L-shaped stirring arm 20 and the second stirring paddle 21 to rotate, the full mixing stirring of samples is realized, in the stirring process, the strip-shaped scraping plate 22 is rotated by the resistance of the sample, or the rotating speed of the L-shaped stirring arm 20 is increased, so that the inertia force is larger than the resistance of the damping shaft 23, the strip-shaped scraping plate 22 is unfolded and is in contact with the inner side wall of the exosome pre-modulation tank 1, the scraping operation is realized, stirring dead angles are avoided, the stirring uniformity is further improved, when the stirring is completed, the rotation speed of the driving motor 9 is increased, the sample adhered to the surfaces of the first stirring paddle 19, the L-shaped stirring arm 20 and the second stirring paddle 21 is thrown out, finally, the electric telescopic rod 5 drives the scraping ring 7 to move downwards, the scraping ring 7 is in contact with the inner side wall of the exosome pre-modulation tank 1 to scrape the material, and the material is concentrated to the bottom end inside the exosome pre-modulation tank 1, so that the discharging effect is improved.
In operation, through setting up the modulation drive assembly that comprises frame type support frame, driving motor, main driving shaft, helical gear one, pivot, helical gear two, stirring center pin, sleeve and helical gear three and the exosome mixing stirring assembly that comprises a plurality of first stirring rake, a plurality of L type stirring arm and a plurality of second stirring rake, can realize mixing stirring to the sample, and then improve the degree of accuracy of detection, avoid causing misdiagnosis; through being provided with electric telescopic handle, scraping material ring, bar scraper blade, damping axle and rubber and scraping the strip to can scrape the material operation to the inside wall of the pre-modulation jar of exosome, avoid the sample adhesion to influence the degree of consistency of mixing at the inside wall of the pre-modulation jar of exosome, can improve the material effect simultaneously.
Claims (5)
1. An exosome detection device for tumour detects and molecular typing, including exosome premodulation jar (1), its characterized in that: the automatic feeding device is characterized in that a discharge hole (2) is formed in the bottom end of the exosome pre-modulation tank (1) in a fixed manner, a modulation driving assembly (3), a material dripping feeding hole (4) and a plurality of electric telescopic rods (5) are fixedly arranged at the top end of the exosome pre-modulation tank (1), an exosome mixing and stirring assembly (6) fixedly connected with the modulation driving assembly (3) is movably arranged in the exosome pre-modulation tank (1), and a scraping ring (7) which is attached to the inner side wall of the exosome pre-modulation tank (1) and fixedly connected with the electric telescopic rods (5) is arranged on the side edge of the interior of the exosome pre-modulation tank (1).
2. The exosome detection apparatus for tumor detection and molecular typing according to claim 1, wherein: modulation drive assembly (3) comprises frame support frame (8), driving motor (9), main drive shaft (10), helical gear one (11), pivot (12), helical gear two (13), stirring center pin (14), sleeve (15) and helical gear three (16), frame support frame (8) fixed connection is in the top of exosome pre-modulation jar (1), driving motor (9) fixed connection is in the intermediate position of frame support frame (8) one side, main drive shaft (10) fixed connection is in the output shaft of driving motor (9), helical gear one (11) fixed connection is in the one end of main drive shaft (10), helical gear two (13) are connected in the inside top of frame support frame (8) through pivot (12), pivot (12) are fixed connection structure with helical gear two (13), stirring center pin (14) fixed connection is in the intermediate position of helical gear two (13) bottom, sleeve (15) alternate in frame support frame (8) and cover are located stirring center pin (14), helical gear three (16) fixed connection is in the top of sleeve (15) and cover is located stirring center pin (14).
3. The exosome detection apparatus for tumor detection and molecular typing according to claim 2, wherein: the main driving shaft (10), the rotating shaft (12), the sleeve (15) and the frame-type supporting frame (8) are movably connected through a first bearing (17), and the stirring central shaft (14) is movably connected with the sleeve (15) and a third bevel gear (16) through a plurality of second bearings (18).
4. The exosome detection apparatus for tumor detection and molecular typing according to claim 2, wherein: the exosome mixing stirring assembly (6) comprises a plurality of first stirring paddles (19), a plurality of L-shaped stirring arms (20) and a plurality of second stirring paddles (21), wherein the first stirring paddles (19) are fixedly connected to the side edges of the stirring central shaft (14), the L-shaped stirring arms (20) are fixedly connected to the outer side edges of the sleeves (15), and the second stirring paddles (21) are fixedly connected to the inner side edges of the L-shaped stirring arms (20) and are alternately distributed with the first stirring paddles (19).
5. The exosome detection apparatus for tumor detection and molecular typing according to claim 4, wherein: a strip-shaped scraping plate (22) is movably arranged on the outer side edge of the L-shaped stirring arm (20), the strip-shaped scraping plate (22) is movably connected with the L-shaped stirring arm (20) through a damping shaft (23), and a rubber scraping strip (24) is fixedly arranged on one side, far away from the L-shaped stirring arm (20), of the strip-shaped scraping plate (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321314230.6U CN219950992U (en) | 2023-05-26 | 2023-05-26 | Exosome detection device for tumor detection and molecular typing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321314230.6U CN219950992U (en) | 2023-05-26 | 2023-05-26 | Exosome detection device for tumor detection and molecular typing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219950992U true CN219950992U (en) | 2023-11-03 |
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ID=88550684
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321314230.6U Active CN219950992U (en) | 2023-05-26 | 2023-05-26 | Exosome detection device for tumor detection and molecular typing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219950992U (en) |
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2023
- 2023-05-26 CN CN202321314230.6U patent/CN219950992U/en active Active
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