CN219907632U - Closed PCR amplification tube - Google Patents
Closed PCR amplification tube Download PDFInfo
- Publication number
- CN219907632U CN219907632U CN202320993056.6U CN202320993056U CN219907632U CN 219907632 U CN219907632 U CN 219907632U CN 202320993056 U CN202320993056 U CN 202320993056U CN 219907632 U CN219907632 U CN 219907632U
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- China
- Prior art keywords
- pcr amplification
- amplification tube
- ring
- tube body
- movable
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- 238000012408 PCR amplification Methods 0.000 title claims abstract description 62
- 238000007789 sealing Methods 0.000 claims abstract description 65
- 230000003321 amplification Effects 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 235000012046 side dish Nutrition 0.000 description 1
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Abstract
The utility model relates to the technical field of PCR amplification and discloses a closed PCR amplification tube which solves the problem of poor sealing performance of the existing PCR amplification tube, and the closed PCR amplification tube comprises a PCR amplification tube body, wherein a sealing mechanism is arranged at the top of the PCR amplification tube body and is provided with a locking mechanism, the sealing mechanism comprises a sealing cover positioned at the top of the PCR amplification tube body, a handle is fixedly arranged at the top of the sealing cover, and an inner sealing ring and an outer sealing ring are fixedly arranged at the bottom of the sealing cover; according to the utility model, the two movable cylinders are conveniently located in the two side grooves respectively through the coordination between the side rods and the rotating sleeve, the top ends of the two inserted rods are conveniently located in the two jacks respectively through the coordination between the movable cylinders and the movable rods and between the bottom block and the spring, the sealing cover and the fixing ring are fixed, and then the inner sealing ring and the outer sealing ring can be respectively fixed on the inner wall and the outer wall of the PCR amplification tube body, so that the sealing performance of the PCR amplification tube body is improved.
Description
Technical Field
The utility model belongs to the technical field of PCR amplification, and particularly relates to a closed PCR amplification tube.
Background
The Polymerase Chain Reaction (PCR) is a molecular biological technology for amplifying specific DNA fragments, and can be regarded as special DNA replication outside organisms, and the greatest characteristic of the PCR is that trace DNA can be greatly increased, so that the PCR can be used for amplifying and comparing as long as the DNA at a single point can be separated; according to the authorized bulletin number: CN211689056U, a patent document entitled "closed PCR amplification tube", describes in the specification: the sealing performance of the connection between the sealing cover and the PCR amplification tube main body can be improved, so that the use of the PCR amplification tube by people is facilitated, and the use requirement of people is further met; the following drawbacks still remain:
the sealing cover is positioned at the top of the PCR amplification tube main body, and the sealing cover is not connected and fixed with the PCR amplification tube main body, so that the sealing cover is easy to move at the top of the PCR amplification tube main body, and the sealing performance of the PCR amplification tube main body is poor.
Disclosure of Invention
Aiming at the situation, in order to overcome the defects of the prior art, the utility model provides the closed PCR amplification tube, which effectively solves the problem of poor sealing property of the existing PCR amplification tube.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the sealed PCR amplification tube comprises a PCR amplification tube body, wherein a sealing mechanism is arranged at the top of the PCR amplification tube body, and a locking mechanism is arranged on the sealing mechanism;
the sealing mechanism comprises a sealing cover positioned at the top of the PCR amplification tube body, a handle is fixedly arranged at the top of the sealing cover, an inner sealing ring and an outer sealing ring are fixedly arranged at the bottom of the sealing cover, the inner sealing ring is attached to the inner wall of the PCR amplification tube body, the outer sealing ring is attached to the outer wall of the PCR amplification tube body, two movable cylinders are symmetrically and movably arranged at the outer side of the sealing cover, movable rods are movably sleeved in the two movable cylinders, bottom blocks are fixedly arranged at the bottom ends of the two movable rods, inserting rods are fixedly arranged at the top of the two bottom blocks, springs are fixedly connected between the two movable cylinders and the two bottom blocks, the two springs are movably sleeved with the two movable rods respectively, a fixing ring is fixedly arranged at the outer side of the PCR amplification tube body, two insertion holes are symmetrically arranged at the bottom of the fixing ring, and the top ends of the two inserting rods are respectively positioned in the two insertion holes.
Preferably, both sides of the fixed ring are provided with side grooves, and the two movable cylinders are respectively positioned in the two side grooves.
Preferably, the outside symmetry fixed mounting of sealed lid has two side pieces, and the equal fixed mounting in one side that two side pieces kept away from each other has the side lever, and the equal fixed mounting in one end that two side levers kept away from each other has the side dish, and the outside of two side levers is all rotated and is connected with the rotation cover, and the top of two movable barrels is respectively with two rotation cover fixed connection.
Preferably, the locking mechanism comprises a mounting ring positioned at the outer side of the PCR amplification tube body, the mounting ring is attached to the fixing ring, two screws are symmetrically and fixedly arranged at one side of the fixing ring, which is close to the mounting ring, the mounting ring is movably sleeved at the outer sides of the two screws, two nuts are symmetrically arranged at one side of the mounting ring, which is far away from the fixing ring, and are respectively sleeved with the two screws in a threaded manner, and two inserting blocks are symmetrically and fixedly arranged at one side of the mounting ring, which is close to the fixing ring.
Preferably, two fixed blocks are fixedly arranged on the outer sides of the movable cylinders, the two fixed blocks are respectively located in the two side grooves, two slots are symmetrically formed in one sides, close to the mounting ring, of the two fixed blocks, and the two inserting blocks are respectively located in the two slots.
Compared with the prior art, the utility model has the beneficial effects that:
(1) According to the utility model, the two movable cylinders are conveniently located in the two side grooves respectively through the coordination between the side rods and the rotating sleeve, the top ends of the two inserted rods are conveniently located in the two jacks respectively through the coordination between the movable cylinders and the movable rods and between the bottom block and the spring, the sealing cover and the fixed ring are fixed, and then the inner sealing ring and the outer sealing ring can be respectively fixed on the inner wall and the outer wall of the PCR amplification tube body, so that the sealing performance of the PCR amplification tube body is improved;
(2) This novel cooperation between passing through collar and solid fixed ring and screw rod and the nut is convenient for the collar and is fixed on solid fixed ring, then makes two inserts be located the inside of two slots respectively to be convenient for carry out spacingly to two movable cylinders, thereby make sealed lid can firmly fix at the top of PCR amplification tube body.
Drawings
The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the embodiments of the utility model, serve to explain the utility model.
In the drawings:
FIG. 1 is a schematic diagram of a closed PCR amplification tube according to the present utility model;
FIG. 2 is a schematic view of a seal mechanism according to the present utility model;
FIG. 3 is a schematic view of a movable barrel structure of the utility model;
FIG. 4 is a schematic view of a retaining ring according to the present utility model;
FIG. 5 is a schematic view of a locking mechanism according to the present utility model;
FIG. 6 is a schematic view of the mounting ring and mounting block of the present utility model in an exploded configuration.
In the figure: 1. a PCR amplification tube body; 2. a sealing mechanism; 201. sealing cover; 202. a handle; 203. a movable cylinder; 204. a fixing ring; 205. an inner seal ring; 206. an outer seal ring; 207. a side block; 208. a rotating sleeve; 209. a side bar; 2010. a side plate; 2011. a rod; 2012. a bottom block; 2013. a movable rod; 2014. a spring; 2015. a side groove; 2016. a jack; 3. a locking mechanism; 301. a mounting ring; 302. a screw; 303. a fixed block; 304. a nut; 305. inserting blocks; 306. a slot.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model; all other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
The first embodiment is given by FIG. 1, the utility model comprises a PCR amplification tube body 1, a sealing mechanism 2 is arranged at the top of the PCR amplification tube body 1, and a locking mechanism 3 is arranged on the sealing mechanism 2;
in the use state, the sealing performance of the PCR amplification tube body 1 is improved through the sealing mechanism 2 and the locking mechanism 3.
Specifically, given by fig. 2-4, sealing mechanism 2 includes sealing cover 201 that is located at the top of PCR amplification tube body 1, handle 202 is fixedly installed at the top of sealing cover 201, inner sealing ring 205 and outer sealing ring 206 are fixedly installed at the bottom of sealing cover 201, inner sealing ring 205 is attached to the inner wall of PCR amplification tube body 1, outer sealing ring 206 is attached to the outer wall of PCR amplification tube body 1, two movable cylinders 203 are symmetrically and movably installed at the outer side of sealing cover 201, movable rods 2013 are movably sleeved in two movable cylinders 203, bottom blocks 2012 are fixedly installed at the bottom ends of two movable rods 2013, inserted bars 2011 are fixedly installed at the top of two bottom blocks 2012, springs 2014 are fixedly connected between two movable cylinders 203 and two bottom blocks 2012, two springs 2014 are respectively and movably sleeved with two movable rods 2013, the outer sides of PCR amplification tube body 1 are fixedly provided with fixing rings 204, two insertion holes are symmetrically arranged at the bottom of fixing rings 204, two sides of fixing rings 204 are respectively and movably sleeved with side slots 2013, two movable cylinders 203 are respectively and rotatably connected with two side shafts 2015, two side shafts 201 are rotatably and rotatably connected with two side shafts 209, and one end of two side shafts 209 are respectively and rotatably connected with two side shafts 209;
in the use state, firstly, the sealing cover 201 is placed at the top of the PCR amplification tube body 1, meanwhile, the inner sealing ring 205 is attached to the inner wall of the PCR amplification tube body 1, the outer sealing ring 206 is attached to the outer wall of the PCR amplification tube body 1, then, the two bottom blocks 2012 are pulled to drive the two movable rods 2013 to move, at the moment, the two springs 2014 are stretched, then, the two movable cylinders 203 are rotated until the two movable cylinders 203 are in a vertical state, meanwhile, the two movable cylinders 203 are respectively positioned in the two side grooves 2015, then, the control on the two bottom blocks 2012 is released, at the moment, the two springs 2014 are reset to drive the two bottom blocks 2012 to move upwards until the top ends of the two inserting rods 2011 are respectively positioned in the two inserting holes 2016, and the sealing cover 201 is fixed with the fixed ring 204, so that the sealing cover 201 is connected with the PCR amplification tube body 1, and the sealing performance of the PCR amplification tube body 1 is improved.
Specifically, as shown in fig. 5-6, the locking mechanism 3 comprises a mounting ring 301 positioned at the outer side of the PCR amplification tube body 1, the mounting ring 301 is attached to the fixing ring 204, two screws 302 are symmetrically and fixedly arranged at one side of the fixing ring 204 close to the mounting ring 301, the mounting ring 301 is movably sleeved at the outer sides of the two screws 302, two nuts 304 are symmetrically arranged at one side of the mounting ring 301 far from the fixing ring 204, the two nuts 304 are respectively in threaded sleeve connection with the two screws 302, two inserting blocks 305 are symmetrically and fixedly arranged at one side of the mounting ring 301 close to the fixing ring 204, fixing blocks 303 are fixedly arranged at the outer sides of the two movable cylinders 203, the two fixing blocks 303 are respectively positioned in two side grooves 2015, two inserting grooves 306 are symmetrically arranged at one side of the two fixing blocks 303 close to the mounting ring 301, and the two inserting blocks 305 are respectively positioned in the two inserting grooves 306;
in a use state, the mounting ring 301 is sleeved on the outer side of the PCR amplification tube body 1, so that the mounting ring 301 is attached to the fixing ring 204, at the moment, the mounting ring 301 is sleeved on the two screws 302, meanwhile, the two inserting blocks 305 are respectively located in the two slots 306, then the mounting ring 301 and the fixing ring 204 are fixed through the two nuts 304, the positions of the two movable cylinders 203 are limited, and finally the sealing cover 201 is firmly installed on the top of the PCR amplification tube body 1, so that the sealing performance of the PCR amplification tube body 1 is improved.
Claims (5)
1. The utility model provides a closed PCR amplification tube, includes PCR amplification tube body (1), its characterized in that: the top of the PCR amplification tube body (1) is provided with a sealing mechanism (2), and the sealing mechanism (2) is provided with a locking mechanism (3);
sealing mechanism (2) including being located sealed lid (201) at PCR amplification tube body (1) top, sealed top fixed mounting of lid (201) has handle (202), sealed bottom fixed mounting of lid (201) has interior sealing washer (205) and outer sealing washer (206), interior sealing washer (205) are laminated with the inner wall of PCR amplification tube body (1), outer sealing washer (206) are laminated with the outer wall of PCR amplification tube body (1), two movable cylinders (203) are installed to the outside symmetry movable mounting of sealed lid (201), movable rod (2013) have all been cup jointed to the inside of two movable cylinders (203), bottom all fixed mounting of two movable rods (2013) has foundation block (2012), the top of two foundation block (2012) all fixed mounting has inserted bar (2011), equal fixedly connected with spring (2014) between two movable cylinders (203) and two foundation block (2012), two springs (2014) are respectively with two movable rod (2013) activity cup joints, fixed ring (204) are installed to the outside fixed mounting of amplification tube body (1), the bottom of fixed ring (204) is equipped with two jack (2016) inside of two jack (2016) respectively.
2. The closed PCR amplification tube as claimed in claim 1, wherein: both sides of the fixed ring (204) are provided with side grooves (2015), and the two movable cylinders (203) are respectively positioned in the two side grooves (2015).
3. The closed PCR amplification tube as claimed in claim 1, wherein: two side blocks (207) are symmetrically and fixedly arranged on the outer side of the sealing cover (201), side rods (209) are fixedly arranged on one sides, away from each other, of the two side blocks (207), side discs (2010) are fixedly arranged on one ends, away from each other, of the two side rods (209), rotating sleeves (208) are rotatably connected on the outer sides of the two side rods (209), and the top ends of the two movable cylinders (203) are fixedly connected with the two rotating sleeves (208) respectively.
4. The closed PCR amplification tube as claimed in claim 1, wherein: the locking mechanism (3) comprises a mounting ring (301) positioned on the outer side of the PCR amplification tube body (1), the mounting ring (301) is attached to the fixing ring (204), two screws (302) are symmetrically and fixedly arranged on one side, close to the mounting ring (301), of the fixing ring (204), the mounting ring (301) is movably sleeved on the outer side of the two screws (302), two nuts (304) are symmetrically arranged on one side, away from the fixing ring (204), of the mounting ring (301), the two nuts (304) are respectively in threaded sleeve connection with the two screws (302), and two inserting blocks (305) are symmetrically and fixedly arranged on one side, close to the fixing ring (204), of the mounting ring (301).
5. The closed PCR amplification tube as claimed in claim 1, wherein: two fixed blocks (303) are fixedly installed on the outer sides of the movable barrels (203), the two fixed blocks (303) are respectively located in two side grooves (2015), two slots (306) are symmetrically formed in one sides, close to the mounting ring (301), of the two fixed blocks (303), and the two inserting blocks (305) are respectively located in the two slots (306).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320993056.6U CN219907632U (en) | 2023-04-27 | 2023-04-27 | Closed PCR amplification tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320993056.6U CN219907632U (en) | 2023-04-27 | 2023-04-27 | Closed PCR amplification tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219907632U true CN219907632U (en) | 2023-10-27 |
Family
ID=88467371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320993056.6U Active CN219907632U (en) | 2023-04-27 | 2023-04-27 | Closed PCR amplification tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219907632U (en) |
-
2023
- 2023-04-27 CN CN202320993056.6U patent/CN219907632U/en active Active
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| GR01 | Patent grant |