CN219898201U

CN219898201U - Chip for detection and analysis provided with sealing body

Info

Publication number: CN219898201U
Application number: CN202321357466.8U
Authority: CN
Inventors: 王志平; 汪椿树; 黄丽; 于坤; 贺黎明
Original assignee: Shenzhen Anlu Medical Technology Co ltd
Current assignee: Shenzhen Anlu Medical Technology Co ltd
Priority date: 2023-05-31
Filing date: 2023-05-31
Publication date: 2023-10-27
Anticipated expiration: 2033-05-31

Abstract

The chip for detection and analysis provided with the sealing body is used for carrying a sample to be subjected to microscopic imaging and comprises a bottom plate, the sealing body and a chip main body; the sealing body accommodating groove is arranged around the sample introduction channel groove, the exhaust channel groove and the sample accommodating groove; the sealing body is placed in the sealing body accommodating groove; the bottom plate covers the sample injection channel groove to form a sample injection channel, covers the exhaust channel groove to form an exhaust channel, and covers the sample accommodating groove to form a sample accommodating cavity; the sample accommodating cavity is communicated with the sample introduction channel and the exhaust channel; the sample inlet channel is communicated with the sample inlet; the exhaust channel is communicated with the exhaust port; the bottom plate and the chip main body form an integral structure through bonding or welding or clamping. The sealing body is embedded into the sealing body accommodating groove, so that the influence of the elastic intermediate layer on the height precision of the sample accommodating cavity is reduced, and the precision of the height of the sample accommodating cavity is mainly controlled by the precision of the corresponding groove on the chip main body.

Description

Chip for detection and analysis provided with sealing body

Technical Field

The present utility model relates to a chip for analysis of a component for carrying a sample-containing mixed liquid to be microimaged, and more particularly, to a chip for analysis of a component for detection provided with a sealing body.

Background

In the prior art, a three-layer structure of a substrate, a middle layer and a top layer is generally arranged in an analysis chip for microscopic imaging, and the height of a sample accommodating cavity is controlled through the structural design of the middle layer. Typically, the upper surface of the substrate and the lower surface of the top layer are planar, and the height of the sample-receiving chamber needs to be controlled by the structural design of the intermediate layer. Since the middle layer usually needs adhesive, and two surfaces of the middle layer need to be connected with the substrate and the top layer respectively, the height precision of the sample accommodating cavity needs to be controlled to reach a very high level, and the difficulty is very high.

In the prior art, the common size of the middle layer is equivalent to that of the bottom plate or the chip main body, and when the middle layer is arranged to be a rigid material, the requirement on adhesion is improved; in order to achieve a good bonding effect, an elastic material is generally adopted to manufacture the middle layer, the elastic material is easy to deform under the pressing action of the substrate and the top layer, the dimensional consistency is difficult to achieve, the requirements on the adhesive glue in the process and even the pressing force are very high, and the consistency of the accuracy of the height of the sample accommodating cavity is difficult to control.

Disclosure of Invention

The utility model aims to solve the technical problem that the defects of the chip for detection and analysis in the prior art are overcome, and the chip for detection and analysis provided with the sealing body is provided with the embedded sealing body, the sealing body is embedded into the accommodating groove of the sealing body, the influence of the elastic intermediate layer on the accuracy of the height of the sample accommodating cavity is reduced, and the accuracy of the height of the sample accommodating cavity is mainly controlled by the accuracy of the corresponding groove on the chip main body.

Only two component bottom plates and a chip main body are needed to construct a sample accommodating cavity for bearing different samples to be microscopically imaged, so that the manufacturing cost can be reduced, and the accuracy of the height of the sample accommodating cavity is improved. The technical scheme for solving the technical problems is that the chip for detection and analysis provided with the sealing body is used for carrying a sample to be subjected to microscopic imaging and comprises a bottom plate, the sealing body and a chip main body; the chip main body comprises a sample introduction channel groove, an exhaust channel groove, a sample accommodating groove and a sealing body accommodating groove; the sealing body accommodating groove is arranged around the sample introduction channel groove, the exhaust channel groove and the sample accommodating groove; the sealing body is placed in the sealing body accommodating groove; the bottom plate covers the sample injection channel groove to form a sample injection channel; the bottom plate covers the exhaust channel groove to form an exhaust channel; the bottom plate covers the sample accommodating groove to form a sample accommodating cavity; the sample accommodating cavity is communicated with the sample introduction channel; the sample accommodating cavity is communicated with the exhaust channel; the sample inlet channel is communicated with the sample inlet; the exhaust channel is communicated with the exhaust port; the bottom plate and the chip main body form an integral structure through bonding or welding or clamping.

The sample inlet and the exhaust port are through holes penetrating through the chip main body; the cross-sectional area of the through hole of the sample inlet is larger than that of the through hole of the exhaust port.

The sealing body is an annular deformable sealing ring, and the width of the sealing body accommodating groove is larger than the ring diameter of the sealing ring.

The sealing body is a hollow sheet-shaped sealing device, and the shape of the sealing body accommodating groove corresponds to the shape of the sealing body.

The upper and lower surfaces of the sealing body are coated with adhesive.

Glue containing grooves are distributed on the connecting surface of the chip main body and the bottom plate. The glue accommodating groove is communicated with the edge of the chip main body.

The sample accommodating groove comprises a supporting bar inside, and a bottom plate covers the sample accommodating groove to form a sample accommodating cavity; the support bar divides the sample-receiving chamber into several communicating chambers.

The top of the supporting bar is adhered with the bottom plate into a whole.

The sample introduction channel groove, the exhaust channel groove and the sample containing groove are communicated with each other to form a groove whole; comprising any one of the following features: characteristic A1: the whole groove is in a spindle shape, and the sample inlet and the gas outlet are respectively communicated with two ends of the spindle shape; feature A2: the whole groove is rectangular, and the sample inlet and the gas outlet are respectively communicated with two ends of the rectangle; feature A3: the whole groove is elliptical, and the sample inlet and the gas outlet are respectively communicated with the ellipse; feature A4: the whole recess is circular, the inlet and the gas vent are circular respectively.

The chip for detection and analysis provided with the sealing body comprises any one of the following characteristics: characteristic B1: the chip is used for imaging and analyzing a blood sample, and the height of the sample accommodating cavity is more than 0.03mm and less than 0.5mm; feature B2: the chip is used for imaging and analyzing a blood sample, and the height of the sample accommodating cavity is more than 0.03mm and less than 0.3mm; feature B3: the chip is used for imaging analysis of urine samples, and the height of the sample accommodating cavity is more than 0.05mm and less than 0.5mm; feature B4: the chip is used for the imaging analysis of the fecal suspension sample, and the height of the sample accommodating cavity is more than 0.03mm and less than 0.5mm.

The technical scheme has the advantages that the sealing body is embedded into the sealing body accommodating groove, so that the influence of the elastic intermediate layer on the height precision of the sample accommodating cavity is reduced, and the precision of the height of the sample accommodating cavity is mainly controlled by the precision of the corresponding groove on the chip main body. The deviation of the height accuracy of the sample-receiving chamber due to the elastic intermediate layer is reduced. The bottom plate and the chip main body are directly bonded, the sealing body prevents liquid from overflowing, the bonding requirement is reduced, and if the clamping device is arranged, the bottom plate and the chip main body are not bonded, and are directly clamped into a whole.

The technical scheme has the advantages that the sample inlet is high relative to the horizontal plane of the sample accommodating cavity, so that liquid can conveniently enter the sample accommodating cavity. The area of the sample inlet is large, so that liquid can be conveniently added.

The technical scheme has the advantages that the left side and the right side of the bottom plate protrude out of the two sides of the chip main body and serve as mounting and fixing edges, so that the clamping position and the entering and exiting of the chip are facilitated.

The sealing body is an annular deformable sealing ring, and the width of the sealing body accommodating groove is larger than the ring diameter of the sealing ring. The sealing body can be directly embedded into the sealing body accommodating groove; the requirement on bonding or welding is reduced, and the production becomes more convenient and faster. The influence of the adhesive layer or the welding layer on the height precision of the sample containing cavity is reduced.

The sealing device has the advantages that when the sealing body is a hollow sheet-shaped sealing device, the double-sided adhesive structure of the sealing body is convenient for production and assembly, and meanwhile, the sealing device can be used for bonding the bottom plate and the chip main body. The bonding or welding area is only the area of the upper surface and the lower surface of the sealing body, the upper surface of the bottom plate and the lower surface of the chip main body are not required to be glued or welded integrally, the technical process and materials are saved, the control difficulty of the bonding layer or the welding layer is reduced, and the overall accuracy control is relatively easier when the area of the bonding layer or the welding layer is smaller. If the bottom plate and the chip main body are connected in a clamping mode, the double-sided adhesive structure of the sealing body can enhance the structural strength and the sealing degree.

The technical scheme has the advantages that the sealing body is arranged in the sealing body accommodating groove, so that the production and the processing are convenient, the complex processing is arranged on one side of the chip main body, the error caused by size matching is reduced, and the overall cavity precision level can be improved. The sealing body may be an integrally formed resilient member.

The technical scheme has the advantages that in the production and processing process, in the extrusion process, the glue accommodating groove is communicated with the outside through the edge of the chip main body, and in the extrusion process, an internal sealing air bag is not formed, so that the probability of chip deformation caused by the internal pressure of the air bag is reduced.

The technical scheme has the advantages that the supporting bars can be longitudinally arranged or transversely arranged, can support the chip main body and prevent the sample accommodating cavity from deforming and collapsing.

The technical scheme has the advantages that the top of the supporting bar is adhered to the bottom plate into a whole, so that the expanding force of the liquid in the accommodating cavity can be counterbalanced, and the expansion deformation of the sample accommodating cavity is prevented.

The technical scheme has the beneficial effects that the overall shape of the groove can be distinguished by setting different shapes according to the detection sample. Such as different overall shapes of the grooves corresponding to different test samples.

The technical scheme has the beneficial effects that the height of the sample accommodating cavity can be set to be different according to the requirement of detecting the sample.

Drawings

FIG. 1 is a top elevation view of one embodiment of a chip for detection and analysis provided with a sealing body;

FIG. 2 is a top perspective orthographic view of one embodiment of a chip for detection and analysis provided with a sealing body;

FIG. 3 is an exploded perspective view of one embodiment of a chip for detection and analysis provided with a sealing body;

FIG. 4 is a perspective view of a seal body;

FIG. 5 is one of the perspective views of the chip body;

FIG. 6 is an exploded perspective view of one embodiment of a chip for detection and analysis provided with a sealing body;

fig. 7 is a second perspective view of the chip body.

Detailed Description

Embodiments of the present utility model are described in further detail below with reference to the drawings.

Referring to fig. 1 to 5, in an embodiment of a chip for detection and analysis provided with a sealing body for carrying a sample to be microimaged, the chip comprises a base plate 200, a sealing body 300, and a chip main body 100; the chip main body 100 comprises a sample introduction channel groove 110, an exhaust channel groove 130, a sample accommodating groove 120 and a sealing body accommodating groove 160; the seal accommodating groove 160 is disposed around the sample introduction channel groove 110, the exhaust channel groove 130, and the sample accommodating groove 120; the sealing body 300 is placed in the sealing body receiving groove 160; the bottom plate 200 covers the sample channel groove 110 to form a sample channel; the bottom plate 200 covers the exhaust passage groove 130 to form an exhaust passage; bottom plate 200 covers sample-receiving recess 120 to form a sample-receiving cavity; the sample accommodating cavity is communicated with the sample introduction channel; the sample accommodating cavity is communicated with the exhaust channel; the sample injection channel is communicated with a sample injection port 111; the exhaust passage communicates with the exhaust port 131; the base plate 200 and the chip body 100 are formed into a unitary structure by bonding or welding.

The sample introduction channel groove 110, the exhaust channel groove 130, the sample receiving groove 120, and the sealing body receiving groove 160 on the chip body 100 may be formed by an integrally formed mold or by machining.

The sealing body is embedded into the sealing body accommodating groove, so that the influence of the elastic intermediate layer on the accuracy of the height of the sample accommodating cavity is reduced, and the accuracy of the height of the sample accommodating cavity is mainly controlled by the accuracy of the corresponding groove on the chip main body. The deviation of the height accuracy of the sample-receiving chamber due to the elastic intermediate layer is reduced. The bottom plate and the chip main body are directly adhered, and the sealing body prevents liquid from overflowing.

The base plate 200 and the chip main body 100 may be connected by bonding or welding, or the base plate 200 and the chip main body 100 may be connected by clamping, and the base plate 200 and the chip main body 100 are not bonded during clamping, and the sealing body 300 is directly clamped into a whole. The welding can adopt a welding mode which is adaptive to the materials, such as ultrasonic welding.

The requirements for bonding and welding are reduced, and the influence of the thickness of the adhesive layer or the welding layer on the accuracy of the height of the sample accommodating cavity is reduced. If the connection mode is a clamping connection mode, the bottom plate and the chip main body are not bonded, and are directly clamped into a whole, so that the connection mode is not influenced by an adhesive layer of the adhesive.

As shown in fig. 2-5, in some embodiments, the seal 300 includes a seal ring 310 and a hollow seal body intermediate region 320. The sealing body 300 is an annular deformable sealing ring, and the width of the sealing body receiving groove 160 is larger than the ring diameter of the sealing ring 310. The sealing ring 310 of the sealing body 300 may be directly inserted into the sealing body receiving groove 160; the sealing body middle region 320 has a hollow structure, so that the bottom plate 200 and the chip main body 100 are directly bonded, and the sealing ring 310 of the sealing body 300 can prevent liquid from overflowing. The requirement for adhesion is reduced, the production becomes more convenient and quick, and the sealing body 300 can be directly embedded. The base plate 200 and the chip main body 100 may be connected by bonding or welding, or the base plate 200 and the chip main body 100 may be connected by clamping, and the base plate 200 and the chip main body 100 are not bonded during clamping, and the sealing body 300 is directly clamped into a whole.

As shown in fig. 2 to 5, in other embodiments, the sealing body 300 is a hollow sheet-shaped sealing device, the shape of the sealing body receiving groove 160 corresponds to the shape of the sealing body, and the upper and lower surfaces of the sealing body 300 are coated with adhesive. The double-sided adhesive structure of the sealing body 300 is convenient for production and assembly, and can be used for bonding the bottom plate 200 and the chip main body 100, and if the bottom plate 200 and the chip main body 100 can be connected in an adhesive or welding mode, the bottom plate 200 and the chip main body 100 can also be connected in a clamping mode; the double-sided adhesive structure of the sealing body 300 can further enhance the connection strength and sealing degree between the base plate 200 and the chip body 100.

As shown in fig. 7, in some embodiments, sample-receiving recess 120 includes support bars 122 therein, and bottom plate 200 covers sample-receiving recess 120 to form a sample-receiving cavity; the support bar 122 divides the sample-receiving chamber into several communicating chambers. The support bar 122 supports the connection between the bottom plate 200 and the chip main body 100, preventing the sample-receiving chamber from deforming and collapsing. In other embodiments, the tops of support bars 122 are bonded to base plate 200 as a single piece. The top of the support bar 122 is bonded to the bottom plate 200 as a whole, so that the expansion force of the liquid in the holding cavity can be counteracted, and the expansion deformation of the sample holding cavity can be prevented.

As in the embodiment of fig. 1 to 5, the sample inlet 111 and the exhaust port 131 are through holes penetrating the chip main body 100. The through-hole cross-sectional area of the sample inlet 111 is larger than the through-hole cross-sectional area of the exhaust port 131. The area of the sample inlet is large, so that liquid can be conveniently added. The sample inlet 111 is arranged on the opposite side of the sample channel groove 110, and the sample inlet 111 is high relative to the horizontal plane of the sample accommodating cavity, so that liquid can conveniently enter the sample accommodating cavity.

As in the embodiment of fig. 1 to 4, the area of the base plate 200 is larger than the area of the chip body 100, the chip body 100 and the sealing body 300 are placed at positions corresponding to the center of the base plate 200, and the left and right sides of the base plate 200 protrude from both sides of the chip body 100. The side wings 210 on both sides of the base plate 200 protruding with respect to the chip body 100 serve as mounting fixing edges for in-and-out control positioning and positioning of imaging focus.

As in the embodiment of fig. 7, glue accommodating grooves 180 are distributed on the connection surface of the chip body 100 and the bottom plate 200. The glue receiving groove 180 communicates with the edge of the chip body 100. In the production and processing process, after the chip main body 100 is coated with glue, the chip main body and the bottom plate 200 are adhered into a whole through extrusion; during the extrusion process, the glue containing groove 180 can contain excess glue, avoiding excess glue from entering the sample containing cavity. In the production and processing, in the viscose extrusion process, the glue accommodating groove 180 is communicated with the outside through the edge of the chip main body 100, and in the extrusion process, an internal sealing air bag is not easy to form, so that the influence of the sealing air bag along with the change of air pressure is reduced, and the probability of chip deformation caused by the internal pressure of the air bag is reduced.

In some embodiments not shown in the drawings, the grooves are in a spindle shape as a whole, and the sample inlet and the gas outlet are respectively communicated with two ends of the spindle shape. In some embodiments not shown in the drawings, the whole of the groove is rectangular, and the sample inlet and the gas outlet are respectively communicated with two ends of the rectangle. In some embodiments not shown in the drawings, the grooves are in an oval shape as a whole, and the sample inlet and the gas outlet are respectively communicated with the oval shape. In some embodiments not shown in the drawings, the grooves are circular as a whole, and the inlet and the outlet are circular respectively.

In some embodiments not shown in the drawings, the chip for detection and analysis provided with the sealing body is used for imaging analysis of a blood sample, and the height of the sample containing cavity is greater than 0.03mm and less than 0.3mm.

In some embodiments not shown in the drawings, the chip for detection and analysis provided with the sealing body is used for urine sample imaging analysis, and the height of the sample accommodating cavity is more than 0.05mm and less than 0.5mm.

In some embodiments not shown in the drawings, the chip for detection and analysis provided with the sealing body is used for the imaging analysis of the fecal suspension sample, and the height of the sample containing cavity is more than 0.03mm and less than 0.5mm.

In the embodiment of the chip for detection and analysis provided with the sealing body as shown in fig. 1 to 7, the chip body 100 is made of a material that is transparent to light, including transparent materials such as glass and transparent plastics; can be manufactured by a die integral forming mode or a machining mode.

The chip for detection and analysis provided with the sealing body is used for carrying a sample to be subjected to microscopic imaging and comprises a bottom plate, the sealing body and a chip main body; the chip main body comprises a sample introduction channel groove, an exhaust channel groove, a sample accommodating groove and a sealing body accommodating groove; the sealing body accommodating groove is arranged around the sample introduction channel groove, the exhaust channel groove and the sample accommodating groove; the sealing body is placed in the sealing body accommodating groove; the bottom plate covers the sample injection channel groove to form a sample injection channel; the bottom plate covers the exhaust channel groove to form an exhaust channel; the bottom plate covers the sample accommodating groove to form a sample accommodating cavity; the sample accommodating cavity is communicated with the sample introduction channel; the sample accommodating cavity is communicated with the exhaust channel; the sample inlet channel is communicated with the sample inlet; the exhaust channel is communicated with the exhaust port; the bottom plate and the chip main body form an integral structure through bonding or welding or clamping. The sealing body is embedded into the sealing body accommodating groove, so that the influence of the elastic intermediate layer on the height precision of the sample accommodating cavity is reduced, and the precision of the height of the sample accommodating cavity is mainly controlled by the precision of the corresponding groove on the chip main body.

The foregoing description is not intended to limit the scope of the utility model, but rather is merely illustrative of the embodiments of the utility model, and all equivalent structures or equivalent flow modifications that may be made by the teachings of the utility model and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the utility model.

Claims

1. A chip for detection and analysis provided with a sealing body, characterized in that:

the sample carrier for microscopic imaging comprises a bottom plate, a sealing body and a chip main body;

the chip main body comprises a sample introduction channel groove, an exhaust channel groove, a sample accommodating groove and a sealing body accommodating groove; the sealing body accommodating groove is arranged around the sample introduction channel groove, the exhaust channel groove and the sample accommodating groove; the sealing body is placed in the sealing body accommodating groove;

the bottom plate covers the sample injection channel groove to form a sample injection channel; the bottom plate covers the exhaust channel groove to form an exhaust channel; the bottom plate covers the sample accommodating groove to form a sample accommodating cavity; the sample accommodating cavity is communicated with the sample introduction channel; the sample accommodating cavity is communicated with the exhaust channel; the sample inlet channel is communicated with the sample inlet; the exhaust channel is communicated with the exhaust port; the bottom plate and the chip main body form an integral structure through bonding or welding or clamping.

2. The chip for detection and analysis provided with a sealing body according to claim 1, wherein the sample inlet and the gas outlet are through holes penetrating through the chip body;

the cross-sectional area of the through hole of the sample inlet is larger than that of the through hole of the exhaust port.

3. The chip for detection and analysis provided with a sealing body according to claim 1, wherein the sealing body is an annular deformable sealing ring, and the width of the sealing body accommodating groove is larger than the ring diameter of the sealing ring.

4. The chip for detection and analysis provided with a sealing body according to claim 1, wherein the sealing body is a hollow sheet-like sealing device, and the shape of the sealing body accommodating groove corresponds to the shape of the sealing body.

5. The chip for detection and analysis provided with a sealing body according to claim 4, wherein the upper and lower surfaces of the sealing body are coated with an adhesive.

6. The chip for inspection and analysis provided with a sealing body according to claim 1, wherein glue containing grooves are distributed on a connection surface of the chip body and the bottom plate, and the glue containing grooves are communicated with an edge of the chip body.

7. The chip for detection and analysis provided with a sealing body according to claim 1, wherein the inside of the sample-receiving groove includes a support bar, and a bottom plate covers the sample-receiving groove to form a sample-receiving cavity; the support bar divides the sample-receiving chamber into several communicating chambers.

8. The chip for detection and analysis provided with a sealing body according to claim 7, wherein the top of the support bar is bonded to the bottom plate as a whole.

9. The chip for detection and analysis provided with a sealing body according to claim 1, wherein the sample introduction channel groove, the exhaust channel groove, and the sample receiving groove are communicated with each other to form a groove body; comprising any one of the following features:

characteristic A1: the whole groove is in a spindle shape, and the sample inlet and the gas outlet are respectively communicated with two ends of the spindle shape;

feature A2: the whole groove is rectangular, and the sample inlet and the gas outlet are respectively communicated with two ends of the rectangle;

feature A3: the whole groove is elliptical, and the sample inlet and the gas outlet are respectively communicated with the ellipse;

feature A4: the whole recess is circular, the inlet and the gas vent are circular respectively.

10. The chip for detection and analysis provided with a sealing body according to claim 1, wherein,

comprising any one of the following features:

characteristic B1: the chip is used for imaging and analyzing a blood sample, and the height of the sample accommodating cavity is more than 0.03mm and less than 0.5mm;

feature B2: the chip is used for imaging and analyzing a blood sample, and the height of the sample accommodating cavity is more than 0.03mm and less than 0.3mm;

feature B3: the chip is used for imaging analysis of urine samples, and the height of the sample accommodating cavity is more than 0.05mm and less than 0.5mm;

feature B4: the chip is used for the imaging analysis of the fecal suspension sample, and the height of the sample accommodating cavity is more than 0.03mm and less than 0.5mm.