CN219897954U - Culture solution mixing device for mesenchymal stem cell culture - Google Patents
Culture solution mixing device for mesenchymal stem cell culture Download PDFInfo
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- CN219897954U CN219897954U CN202320940978.0U CN202320940978U CN219897954U CN 219897954 U CN219897954 U CN 219897954U CN 202320940978 U CN202320940978 U CN 202320940978U CN 219897954 U CN219897954 U CN 219897954U
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- connecting rod
- mixing device
- wall
- culture solution
- mesenchymal stem
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- 238000004113 cell culture Methods 0.000 title claims abstract description 16
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 17
- 238000005096 rolling process Methods 0.000 claims abstract description 13
- 230000005540 biological transmission Effects 0.000 claims description 11
- 241000883990 Flabellum Species 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 description 3
- 238000007790 scraping Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a culture solution mixing device for mesenchymal stem cell culture, which comprises a mixing device, wherein a device top cover is arranged at the upper end of the mixing device, a connecting rod A and a connecting rod B are respectively arranged at the upper end and the lower end of the device top cover below the mixing device, a connecting seat is arranged at the intersection of the connecting rod A and the connecting rod B, and stirring fan blades are arranged at the outer sides of the connecting rod A and the connecting rod B. According to the utility model, when the connecting rod A drives the connecting rod B to perform reverse rotation through the connecting seat, so that convection is formed between the connecting rod A and the stirring fan blades on the outer side of the connecting rod B, the stirring quality of the mixing device is conveniently improved, the wall scraper is arranged at the bottom end of the connecting rod B, and when the connecting rod B drives the wall scraper to rotate, the inner wall of the mixing device is cleaned through the rolling brush, so that the culture solution is prevented from remaining on the inner wall of the mixing device, and the subsequent cleaning is inconvenient.
Description
Technical Field
The utility model relates to the technical field of culture solutions, in particular to a culture solution mixing device for mesenchymal stem cell culture.
Background
Cell culture is also called a cell cloning technology, a normal term in biology is a cell culture technology, and cell culture is an indispensable process for the whole biological engineering technology or one of the biological cloning technologies;
in the prior art, a test cup is often used for mixing culture solutions during cell culture, and after various culture solutions are added, the culture solutions are stirred and mixed by a mixing device.
However, the mixing mechanism inside the conventional culture solution mixing device is not uniform enough to affect the culture quality of cell culture, and after the mixing is finished, the culture solution remains on the inner wall of the culture solution mixing device, so that the subsequent cleaning is inconvenient; therefore, the prior requirements are not met, and a culture solution mixing device for culturing mesenchymal stem cells is provided.
Disclosure of Invention
The utility model aims to provide a culture solution mixing device for mesenchymal stem cell culture, which solves the problems that the mixing mechanism inside the conventional culture solution mixing device proposed in the background art is not uniform enough to affect the culture quality of cell culture, and the culture solution remains on the inner wall of the culture solution mixing device after the mixing is finished, so that the subsequent cleaning is inconvenient.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a culture solution mixing arrangement that mesenchymal stem cell cultivateed usefulness, includes mixing arrangement, mixing arrangement's upper end is equipped with the device top cap, the lower extreme that the device top cap lies in is equipped with connecting rod A and connecting rod B respectively at mixing arrangement's upper and lower both ends, connecting rod A and connecting rod B's crossing department is equipped with the connecting seat, connecting rod A and connecting rod B's the outside all is equipped with stirring vane, connecting rod B's bottom is equipped with the wall and scrapes, the one end that the wall scraped is equipped with the round brush.
Preferably, the upper end of device top cap is equipped with the rotating electrical machines, the bottom of rotating electrical machines is equipped with the transfer line, the transfer line runs through the device top cap and extends to connecting rod A's one end, the rotating electrical machines passes through the transfer line and is connected with connecting rod A transmission.
Preferably, the connecting rod A and one end of the connecting rod B are provided with a gear A on the inner side of the connecting seat, one of the gears A is provided with a gear B on the inner wall of the connecting seat, the gear A is meshed with the gear B, and the connecting rod A and the connecting rod B are connected through the transmission of the connecting seat.
Preferably, a rotating shaft is arranged at the center of the rolling brush, the rotating shaft penetrates through the rolling brush and extends to the inner side of the wall scraper, and the rolling brush is connected with the wall scraper through the rotating shaft.
Preferably, the two sides of the mixing device are provided with feed inlets, the upper ends of the feed inlets are provided with through holes, the through holes extend to the inner sides of the mixing device, and the feed inlets are in through connection with the mixing device through the through holes.
Preferably, a discharge port is arranged at the bottom end of the front end face of the mixing device, and a valve switch is arranged at the upper end of the discharge port.
Compared with the prior art, the utility model has the beneficial effects that:
1. according to the utility model, when the connecting rod A drives the connecting rod B to perform reverse rotation through the connecting seat, so that convection is formed between the connecting rod A and the stirring fan blades on the outer side of the connecting rod B, the stirring quality of the mixing device is conveniently improved, the wall scraper is arranged at the bottom end of the connecting rod B, and when the connecting rod B drives the wall scraper to rotate, the inner wall of the mixing device is cleaned through the rolling brush, so that the culture solution is prevented from remaining on the inner wall of the mixing device, and the subsequent cleaning is inconvenient.
Drawings
FIG. 1 is a schematic view of the overall structure of the present utility model;
FIG. 2 is a schematic diagram of the overall cross-sectional elevation structure of the present utility model;
FIG. 3 is a schematic view of a partial construction of a wall wiper and roll brush junction according to the present utility model;
fig. 4 is a schematic view of the internal partial structure of the connecting seat of the present utility model.
In the figure: 1. a mixing device; 2. a device top cover; 3. a rotating electric machine; 4. a feed inlet; 5. a discharge port; 6. a transmission rod; 7. a connecting rod A; 8. a connecting seat; 9. a connecting rod B; 10. wall scraping; 11. a rolling brush; 12. a gear A; 13. a gear B; 14. stirring fan blade.
Description of the embodiments
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
The rotating electrical machine 3 (model HG-KN 73J-S100) according to the present utility model is commercially available or custom-made.
Referring to fig. 1 to 4, an embodiment of the present utility model provides: the utility model provides a culture solution mixing arrangement that mesenchymal stem cell cultivateed usefulness, including mixing arrangement 1, the upper end of mixing arrangement 1 is equipped with device top cap 2, the lower extreme of device top cap 2 is equipped with connecting rod A7 and connecting rod B9 respectively, the crossing department of connecting rod A7 and connecting rod B9 is equipped with connecting seat 8, the outside of connecting rod A7 and connecting rod B9 all is equipped with stirring flabellum 14, the bottom of connecting rod B9 is equipped with wall and scrapes 10, the one end that the wall scraped 10 is equipped with round brush 11, wholly when connecting rod A7 passes through connecting seat 8 and drives connecting rod B9 and carry out reverse rotation, thereby make connecting rod A7 and the stirring flabellum 14 in the connecting rod B9 outside form the convection current, be convenient for improve mixing arrangement 1's stirring quality, and the bottom of connecting rod B9 is equipped with wall and scrape 10, connecting rod B9 drives wall and scrapes 10 when rotatory, clear up the inner wall of mixing arrangement 1 through round brush 11, prevent that the culture solution from remaining in the mixing arrangement inner wall, be unfavorable for follow-up clear up.
Further, the upper end of device top cap 2 is equipped with rotating electrical machines 3, the bottom of rotating electrical machines 3 is equipped with transfer line 6, transfer line 6 runs through the one end that device top cap 2 extends to connecting rod A7, rotating electrical machines 3 is connected through transfer line 6 and connecting rod A7 transmission, connecting rod A7 and connecting rod B9's one end is located the inboard of connecting seat 8 and is equipped with gear A12, one of them gear A12's both sides are located the inner wall of connecting seat 8 all and are equipped with gear B13, gear A12 and gear B13 intermeshing, connecting rod A7 and connecting rod B9 pass through connecting seat 8 transmission and are connected.
Through adopting above-mentioned technical scheme, and then when rotating electrical machines 3 drove connecting rod A7 and rotate, connecting rod A7 drives connecting rod B9 through connecting seat 8 and carries out reverse rotary motion to make connecting rod A7 and the stirring flabellum 14 in the connecting rod B9 outside form the convection current, be convenient for improve mixing arrangement 1's stirring quality.
Further, a rotating shaft is provided at the center of the rolling brush 11, and extends to the inner side of the wall wiper 10 through the rolling brush 11, and the rolling brush 11 is connected with the wall wiper 10 through the rotating shaft.
Through adopting above-mentioned technical scheme, and then through wall scraping 10 when rotatory, clear up mixing arrangement 1's inner wall through round brush 11, prevent that the culture solution from remaining at mixing arrangement inner wall, be inconvenient for follow-up clearance.
Further, both sides of the mixing device 1 are provided with a feed inlet 4, the upper end of the feed inlet 4 is provided with a through hole, the through hole extends to the inner side of the mixing device 1, the feed inlet 4 is in through connection with the mixing device 1 through the through hole, the bottom end of the front end face of the mixing device 1 is provided with a discharge outlet 5, and the upper end of the discharge outlet 5 is provided with a valve switch.
By adopting the technical scheme, the mixing device 1 is further convenient to feed and discharge.
Working principle: when the stirring device is used, whether all parts are intact is detected, after detection, culture solution to be stirred and mixed is injected into the mixing device 1 from the feed inlet 4, a power supply is connected, a starting device is arranged above the mixing device 1, the rotating motor 3 drives the connecting rod A7 to rotate through the transmission rod 6, the connecting rod A7 drives the connecting rod B9 to conduct reverse rotation through the connecting seat 8, so that the connecting rod A7 and the stirring fan blade 14 on the outer side of the connecting rod B9 form convection currents, the stirring quality of the mixing device 1 is convenient to improve, the wall scraper 10 is arranged at the bottom end of the connecting rod B9, the connecting rod B9 drives the wall scraper 10 to clean the inner wall of the mixing device 1 through the rolling brush 11 when rotating, the culture solution is prevented from remaining on the inner wall of the mixing device, the subsequent cleaning is inconvenient, and after stirring is completed, a valve switch is opened, and the stirred culture solution is taken out.
It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (6)
1. Culture solution mixing arrangement that mesenchymal stem cell cultivateed usefulness includes mixing arrangement (1), its characterized in that: the upper end of mixing arrangement (1) is equipped with device top cap (2), the lower extreme of device top cap (2) below mixing arrangement (1) is equipped with connecting rod A (7) and connecting rod B (9) respectively, the crossing department of connecting rod A (7) and connecting rod B (9) is equipped with connecting seat (8), the outside of connecting rod A (7) and connecting rod B (9) all is equipped with stirring flabellum (14), the bottom of connecting rod B (9) is equipped with wall and scrapes (10), the one end that wall scraped (10) is equipped with round brush (11).
2. The culture solution mixing device for mesenchymal stem cell culture according to claim 1, wherein: the device is characterized in that a rotating motor (3) is arranged at the upper end of the device top cover (2), a transmission rod (6) is arranged at the bottom end of the rotating motor (3), the transmission rod (6) penetrates through the device top cover (2) and extends to one end of a connecting rod A (7), and the rotating motor (3) is in transmission connection with the connecting rod A (7) through the transmission rod (6).
3. The culture solution mixing device for mesenchymal stem cell culture according to claim 1, wherein: the connecting rod A (7) and one end of the connecting rod B (9) are located on the inner side of the connecting seat (8) and are provided with gears A (12), one of the gears A (12) is located on the inner wall of the connecting seat (8), the gears A (12) are meshed with the gears B (13), and the connecting rod A (7) and the connecting rod B (9) are connected through the transmission of the connecting seat (8).
4. The culture solution mixing device for mesenchymal stem cell culture according to claim 1, wherein: the center position of the rolling brush (11) is provided with a rotating shaft, the rotating shaft penetrates through the rolling brush (11) and extends to the inner side of the wall scraper (10), and the rolling brush (11) is connected with the wall scraper (10) through the rotating shaft.
5. The culture solution mixing device for mesenchymal stem cell culture according to claim 1, wherein: both sides of mixing arrangement (1) all are equipped with feed inlet (4), feed inlet (4) upper end is equipped with the through-hole, the through-hole extends to the inboard of mixing arrangement (1), feed inlet (4) are through-hole and mixing arrangement (1) through-connection.
6. The culture solution mixing device for mesenchymal stem cell culture according to claim 1, wherein: the bottom of the front end face of the mixing device (1) is provided with a discharge hole (5), and the upper end of the discharge hole (5) is provided with a valve switch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320940978.0U CN219897954U (en) | 2023-04-24 | 2023-04-24 | Culture solution mixing device for mesenchymal stem cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320940978.0U CN219897954U (en) | 2023-04-24 | 2023-04-24 | Culture solution mixing device for mesenchymal stem cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219897954U true CN219897954U (en) | 2023-10-27 |
Family
ID=88428890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320940978.0U Active CN219897954U (en) | 2023-04-24 | 2023-04-24 | Culture solution mixing device for mesenchymal stem cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219897954U (en) |
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2023
- 2023-04-24 CN CN202320940978.0U patent/CN219897954U/en active Active
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