CN219886036U - Microfluidic chip and microfluidic system - Google Patents
Microfluidic chip and microfluidic system Download PDFInfo
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- CN219886036U CN219886036U CN202223125349.7U CN202223125349U CN219886036U CN 219886036 U CN219886036 U CN 219886036U CN 202223125349 U CN202223125349 U CN 202223125349U CN 219886036 U CN219886036 U CN 219886036U
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- 238000004140 cleaning Methods 0.000 claims abstract description 83
- 238000005336 cracking Methods 0.000 claims abstract description 58
- 238000010828 elution Methods 0.000 claims abstract description 57
- 239000000758 substrate Substances 0.000 claims abstract description 48
- 230000003321 amplification Effects 0.000 claims abstract description 37
- 238000000605 extraction Methods 0.000 claims abstract description 37
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 37
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 238000010438 heat treatment Methods 0.000 claims description 81
- 238000007789 sealing Methods 0.000 claims description 26
- 239000012188 paraffin wax Substances 0.000 claims description 24
- 239000003292 glue Substances 0.000 claims description 16
- 239000005662 Paraffin oil Substances 0.000 claims description 6
- 239000011324 bead Substances 0.000 claims description 6
- 230000000903 blocking effect Effects 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 238000005406 washing Methods 0.000 abstract description 9
- 238000000197 pyrolysis Methods 0.000 abstract description 8
- 238000001514 detection method Methods 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 230000004888 barrier function Effects 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000007731 hot pressing Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Abstract
The utility model provides a microfluidic chip and a microfluidic system, wherein the chip comprises a cracking cavity, a first valve chamber, a cleaning cavity, a second valve chamber, an elution cavity, a liquid path cavity and an amplification cavity, wherein micro valves are arranged in the first valve chamber and the second valve chamber; the microfluidic chip comprises a chip substrate; be provided with pyrolysis zone heater strip, pyrolysis zone thermistor, washing zone heater strip, washing zone thermistor, elution zone heater strip, elution zone thermistor, amplification zone heater strip and amplification zone thermistor on the chip base plate, pyrolysis zone heater strip and pyrolysis zone thermistor all are located under the pyrolysis chamber, washing zone heater strip and washing zone thermistor all are located under the washing chamber, elution zone heater strip and elution zone thermistor all are located under elution chamber and the liquid way chamber. The microfluidic chip is convenient to manufacture, the extraction efficiency is greatly improved, and the detection progress is effectively accelerated.
Description
Technical Field
The utility model relates to the technical field of microfluidics, in particular to a microfluidic chip and a microfluidic system.
Background
The polymerase chain reaction (po l ymerase cha i n react i on, PCR) is a core technology of modern molecular biology and diagnostics. This temperature-dependent technique exponentially amplifies DNA by repeated cycling of heating and cooling. Conventional thermocyclers are bulky and typical PCR usually takes 12 hours. In clinical point-of-care diagnostics, especially for infectious diseases, reaction time, reaction specificity, and instrument portability are critical for quickly making clinical decisions at the point of care (po i nt of care). Many rapid PCR methods have been developed and applied to real-time diagnostics using microfluidic technology or the miniaturized nature of capillary reactors.
The existing microfluidic chip comprises a cover plate and a chip substrate, wherein a cracking cavity, a cleaning cavity, an elution cavity, a liquid path cavity and an amplification cavity are formed between the cover plate and the chip substrate. The chip substrate is provided with a heating wire, and the heating wire is positioned right below the amplification cavity. And the existing microfluidic chip is usually cracked by adding a sample to be detected with magnetic beads into a cracking cavity in a mode of an air gun or an injector. However, the speed of extracting the sample to be detected of the microfluidic chip is low, the sample with the magnetic beads is dispersed around when the sample to be detected is added, and the magnetic device drives the magnetic beads to move, so that the risk of omission exists, the sample detection time is long, and the detection result is inaccurate.
In addition, in the existing processing method of the microfluidic chip, after the whole microfluidic chip is processed, pre-adding of the cracking liquid, the cleaning liquid and the like is performed, pre-storing of the reagent cannot be performed before the glue between the cover plate and the chip substrate is solidified, so that the production efficiency is low, most of the cover plates of the existing microfluidic chip are of an integrated structure, and the cover plates cannot be detached and installed and are low in universality.
Disclosure of Invention
In order to solve the above problems of the prior art, a first object of the present utility model is to provide a microfluidic chip for accelerating the sample extraction speed.
A second object of the present utility model is to provide a microfluidic system comprising a microfluidic chip as described above.
In order to achieve the first purpose, the microfluidic chip provided by the utility model comprises a cracking cavity, a first valve chamber, a cleaning cavity, a second valve chamber, an elution cavity, a liquid path cavity and an amplification cavity which are sequentially communicated, wherein micro valves are arranged in the first valve chamber and the second valve chamber; the microfluidic chip further comprises a chip substrate, wherein an electric connection port, an amplification region heating wire and an amplification region thermistor are arranged on the chip substrate, the amplification region heating wire is positioned right below the amplification cavity and is electrically connected with the electric connection port, and the amplification region heating wire is close to the amplification cavity relative to the amplification region thermistor; the chip substrate is internally provided with a cracking zone heating wire, a cracking zone thermistor, a cleaning zone heating wire, a cleaning zone thermistor, an elution zone heating wire and an elution zone thermistor, wherein the cracking zone heating wire, the cracking zone thermistor, the cleaning zone heating wire, the cleaning zone thermistor, the elution zone heating wire and the elution zone thermistor are electrically connected with the electric connection port; the cracking zone heating wire and the cracking zone thermistor are both positioned right below the cracking cavity, and the cracking zone heating wire is close to the cracking cavity relative to the cracking zone thermistor; the cleaning zone heating wire and the cleaning zone thermistor are both positioned right below the cleaning cavity, and the cleaning zone heating wire is close to the cleaning cavity relative to the cleaning zone thermistor; the elution zone heating wire and the elution zone thermistor are both positioned under the elution cavity and the liquid path cavity, and the elution zone heating wire is close to the elution cavity relative to the elution zone thermistor.
From the above, the provided pyrolysis zone heating wire, pyrolysis zone heat name resistor, cleaning zone heating wire, cleaning zone thermistor, elution zone heating wire and elution zone thermistor can provide proper temperature for pyrolysis process, washing process and elution process, thereby accelerating extraction efficiency and greatly shortening detection time.
The microfluidic chip comprises an extraction cover plate, wherein the extraction cover plate is arranged on a chip substrate; a cracking groove with an opening at the bottom, a first valve groove with an opening at the bottom, a cleaning groove with an opening at the bottom and a second valve groove with an opening at the bottom which are sequentially communicated are arranged on one side of the extraction cover plate adjacent to the chip substrate; the side of the extraction cover plate, which is away from the chip substrate, is provided with a first sample adding hole, a second sample adding hole and an air hole, wherein the first sample adding hole is communicated with the cracking groove, the second sample adding hole is communicated with the cleaning cavity, and the air hole is positioned between the cleaning cavity and the second valve chamber and is communicated with the cleaning cavity and the second valve chamber; the microfluidic chip further comprises a sealing layer, the sealing layer is arranged between the extraction cover plate and the chip substrate, the sealing layer covers the bottom opening of the cracking groove to form a cracking cavity, the sealing layer covers the bottom opening of the first valve groove to form a first valve chamber, the sealing layer covers the bottom opening of the cleaning groove to form a cleaning cavity, and the sealing layer covers the bottom opening of the second valve groove to form a second valve chamber.
From the above, draw the sealing layer that sets up between apron and the chip base plate for draw apron and sealing layer and combine into an organic wholely in advance, be applicable to the micro-fluidic chip of multiple different specifications, promoted its commonality greatly, can advance draw the internal reagent that prestores of combination of apron and sealing layer to install on the chip base plate again, promoted production efficiency greatly, when the gas pocket was used for balanced prestored reagent, the atmospheric pressure in each cavity.
The extraction cover plate is characterized in that at least one glue overflow groove is further formed in one side, adjacent to the chip substrate, of the extraction cover plate, one glue overflow groove is arranged on the outer sides of the cracking groove, the first valve groove, the cleaning groove and the second valve groove in a surrounding mode, and gaps are formed between the glue overflow groove and the cracking groove, between the glue overflow groove and the first valve groove, between the glue overflow groove and the second valve groove.
From the above, through the glue overflow groove that sets up for draw between apron and the chip base plate fixed more firmly.
The microfluidic chip further comprises a conductive cover plate and a blocking layer, wherein the conductive cover plate is arranged on the chip substrate and is adjacent to the extraction cover plate, the blocking layer is arranged between the conductive cover plate and the chip substrate, and an elution cavity, a liquid path cavity and an amplification cavity are formed by enclosing the conductive cover plate, the blocking layer and the chip substrate; the chip substrate further comprises an electrode array, the electrode array is located under the elution cavity, the liquid path cavity and the amplification cavity, and the electrode array is close to the conductive cover plate relative to the heating wires of the elution area and the heating wires of the amplification area.
From the above, the electrode array is arranged below the elution cavity, so that the liquid in the elution cavity can fully move into the liquid path cavity.
The micro valve comprises paraffin oil, and a first paraffin valve heating wire, a first thermistor, a second paraffin valve heating wire and a second thermistor are further arranged on the chip substrate and are electrically connected with the electric connection port; the first paraffin valve heating wire is positioned right below the first valve chamber, the first paraffin valve heating wire is close to the first valve chamber relative to the first thermistor, the second paraffin valve heating wire is positioned right below the second valve chamber, and the second paraffin valve heating wire is close to the second valve chamber relative to the second thermistor; one side of the extraction cover plate, which is away from the chip substrate, is provided with a third sample adding hole and a fourth sample adding hole, the third sample adding hole is communicated with the first valve chamber, and the fourth sample adding hole is communicated with the second valve chamber.
The further scheme is that the number of the cleaning cavities can be more than two, and the extraction cover plate is provided with second sample adding holes which are the same as the number of the cleaning cavities and correspond to each other one by one.
From the above, a plurality of samples to be tested are cracked and then enter a plurality of cleaning cavities, so that impurities can be sufficiently washed away, and the interference of the impurities on test results is reduced.
In order to achieve the second object, the microfluidic system provided by the utility model comprises a first magnetic device, a second magnetic device and any one of the microfluidic chips; the first magnetic device is positioned right below the microfluidic chip, and the second magnetic device is positioned right above the microfluidic chip; the first magnetic device comprises a first magnet and a second magnet, and the diameter of the first magnet is larger than that of the second magnet; the first magnetic device and the second magnetic device can move along the length direction, the width direction and the thickness direction of the microfluidic chip; the second magnet controls the sample to be tested with the magnetic beads to move from the lysis chamber to the elution chamber.
In summary, the microfluidic chip provided by the scheme is convenient to manufacture, so that the extraction efficiency is greatly improved, and the detection progress is effectively accelerated.
Drawings
Fig. 1 is an exploded view of an embodiment of a microfluidic chip of the present utility model.
Fig. 2 is a plan view of a side of an extraction cover plate adjacent to a chip substrate in accordance with an embodiment of the present utility model.
Fig. 3 is a cross-sectional view of an embodiment of a microfluidic chip of the present utility model including a lysis chamber, a wash chamber, and an elution chamber.
Fig. 4 is a partial cross-sectional view of an embodiment of a microfluidic chip of the present utility model including a liquid path chamber and an amplification chamber.
Fig. 5 is a schematic plan view of an embodiment of a microfluidic chip according to the present utility model.
Fig. 6 is a layout of heater wires on a chip substrate of an embodiment of a microfluidic chip according to the present utility model.
Detailed Description
Referring to fig. 1 to 6, the microfluidic chip provided in this embodiment includes an extraction cover plate 2, a conductive cover plate 3, a chip substrate 1, a sealing layer 4, and a barrier layer 12, where the conductive cover plate 3 and the extraction cover plate 2 are both disposed on the chip substrate 1, the conductive cover plate 3 is adjacent to the extraction cover plate 2, the sealing layer 4 is located between the extraction cover plate 2 and the chip substrate 1, and the barrier layer 12 is located between the conductive cover plate 3 and the chip substrate 1. The chip substrate 1 is provided with an electrical connection port 9, an electrode array 11, an amplification region heating wire 310 and an amplification region thermistor 311, and the electrode array 11, the amplification region heating wire 310 and the amplification region thermistor 311 are electrically connected with the electrical connection port 9. The sealing layer 4 can be a plastic film made of nontoxic medical plastic materials such as PE, PP and the like, and the barrier layer 12 comprises a gasket and glue.
In this embodiment, a cleavage groove with an opening at the bottom, a first valve groove with an opening at the bottom, a first cleaning groove with an opening at the bottom, a second cleaning groove with an opening at the bottom, a third cleaning groove with an opening at the bottom, and a second valve groove with an opening at the bottom are sequentially arranged on one side of the extraction cover plate 2 adjacent to the chip substrate 1, a first sample adding hole 50, three second sample adding holes and an air hole 20 are arranged on one side of the extraction cover plate 2, which is far away from the chip substrate 1, the first sample adding hole 50 is communicated with the cleavage groove, the second sample adding holes are communicated with the cleaning groove, and the air hole 20 is positioned between the third cleaning groove and the second valve groove and is communicated with the third cleaning groove and the second valve groove. The sealing layer 4 covers the bottom opening of the cracking groove to form a cracking cavity 5, the sealing layer 4 covers the bottom opening of the first valve groove to form a first valve chamber 6, the sealing layer 4 covers the bottom opening of the first cleaning groove to form a first cleaning cavity 7-1, the sealing layer 4 covers the bottom opening of the second cleaning groove to form a second cleaning cavity 7-2, the sealing layer 4 covers the bottom opening of the third cleaning groove to form a third cleaning cavity 7-3, the sealing layer 4 covers the bottom opening of the second valve groove to form a second valve chamber 8, and micro valves are arranged in the first valve chamber 6 and the second valve chamber 8. One second well 70-1 communicates with the first washing chamber 7-1, one second well 70-2 communicates with the second washing chamber 7-2, and the last second well 70-3 communicates with the third washing chamber 7-3.
Optionally, the number of the cleaning tanks is at least one, and the number of the second sample adding holes is the same as and corresponds to the number of the cleaning tanks one by one.
Referring to fig. 4, in this embodiment, the conductive cover plate 3, the barrier layer 12 and the chip substrate 1 enclose an elution chamber 10, a liquid path chamber 30 and an amplification chamber 31, which are sequentially communicated. The electrode array 11 is located directly below the elution chamber 10, the liquid path chamber 30 and the amplification chamber 31. The chip substrate 1 is also provided with a cracking zone heating wire 500, a cracking zone thermistor 501, a cleaning zone heating wire 700, a cleaning zone thermistor 701, an elution zone heating wire 300 and an elution zone thermistor 301. The cracking zone heating wire 500, the cracking zone thermistor 501, the cleaning zone heating wire 700, the cleaning zone thermistor 701, the elution zone heating wire 300 and the elution zone thermistor 301 are all electrically connected with the electrical connection port 9, the cracking zone thermistor 501 is used for detecting the temperature of the cracking zone heating wire 500, the cleaning zone thermistor 701 is used for detecting the temperature of the cleaning zone heating wire 700, and the elution zone thermistor 301 is used for detecting the temperature of the elution zone heating wire 300. The cracking zone heating wire 500 and the cracking zone thermistor 501 are both located directly below the cracking chamber 5, and the cracking zone heating wire 500 is close to the cracking chamber 5 relative to the cracking zone thermistor 501. The cleaning zone heating wire 700 and the cleaning zone thermistor 701 are respectively positioned under the first cleaning cavity 7-1, the second cleaning cavity 7-2 and the third cleaning cavity 7-3, and the cleaning zone heating wire 700 is close to the extraction cover plate 2 relative to the cleaning zone thermistor 701. The elution zone heating wire 300 and the elution zone thermistor 301 are both located directly below the elution chamber 10 and the liquid path chamber 30, and the elution zone heating wire 300 is close to the elution chamber 10 relative to the elution zone thermistor 301.
Referring to fig. 2 and 5, in this embodiment, the micro valve is paraffin oil, and the chip substrate 1 is further provided with a first paraffin valve heating wire 600, a first thermistor 601, a second paraffin valve heating wire 800, and a second thermistor 801, where the first paraffin valve heating wire 600, the first thermistor 601, the second paraffin valve heating wire 800, and the second thermistor 801 are all electrically connected to the electrical connection port 9. The first paraffin valve heating wire 600 is located directly under the first valve chamber 6, the first paraffin valve heating wire 600 is close to the first valve chamber 6 with respect to the first thermistor 601 and is used for detecting the temperature of the first paraffin valve heating wire 600, the second paraffin valve heating wire 800 is located directly under the second valve chamber 8, and the second paraffin valve heating wire 800 is close to the second valve chamber 8 with respect to the second thermistor 801 and is used for detecting the temperature of the second paraffin valve heating wire 800. The side of the extraction cover plate 2, which is away from the chip substrate 1, is provided with a third sample adding hole 60 and a fourth sample adding hole 80, wherein the third sample adding hole 60 is communicated with the first valve chamber 6, and the fourth sample adding hole 80 is communicated with the second valve chamber 8. Alternatively, the micro-valve may be a mechanical valve or the like.
In this embodiment, six glue overflow grooves 21 are disposed on one side of the extraction cover plate 2 adjacent to the chip substrate 1, wherein one glue overflow groove 21 is disposed around the outside of the cracking groove, the first valve groove, the first cleaning groove, the second cleaning groove, the third cleaning groove and the second valve groove, and gaps are formed between the glue overflow groove 21 and the cracking groove, the first valve groove, the cleaning groove and the second valve groove.
The embodiment also provides a manufacturing method for manufacturing the microfluidic chip, which comprises the following steps: firstly, the sealing layer 4 and the extraction cover plate 2 are combined into a whole in a hot pressing mode, paraffin oil is added into the first valve chamber 6 through the third sample adding hole 60, and after the paraffin oil is solidified, the paraffin oil is added into the second valve chamber 8 through the fourth sample adding hole 80. Then adding the lysate into the lysis chamber 5 through the first sample adding holes 50, adding the cleaning liquid into the cleaning chambers through the second sample adding holes, and finally mounting the combination of the sealing layer 4 and the extraction cover plate 2 on the chip substrate 1.
Referring to fig. 3 and 4, the present embodiment provides a microfluidic system including the above-described microfluidic chip, the system including a first magnetic device 100, a second magnetic device 101, and a fluorescence detection device 200, the first magnetic device 100 being located directly below the microfluidic chip, the second magnetic device 101 being located directly above the microfluidic chip; the first magnetic device 100 includes a first magnet 1000 and a second magnet 1001, the first magnet 1000 having a larger diameter than the second magnet 1001; the first magnetic device 100 and the second magnetic device 101 are each movable in the length direction, the width direction, and the thickness direction of the microfluidic chip; the second magnet 1001 controls the movement of the sample to be measured with magnetic beads from the lysis chamber 5 to the elution chamber 10, and the fluorescence detection device 200 is located directly above the microfluidic chip and directly above the amplification chamber 31.
The embodiment also provides a working method of the microfluidic system, which comprises the following steps: the first step: adding a sample to be tested into the cracking cavity 5 for cracking, then moving the first magnet 1000 to the position right below the cracking cavity 5 for carrying out primary enrichment on the cracked sample to be tested, removing the first magnet 1000, and carrying out secondary enrichment on the cracked sample to be tested by the second magnet 1001; and a second step of: the second magnet 1001 controls the sample to be tested to move into the cleaning cavity through the first valve chamber 6, the second magnet 1001 and the second magnetic device 101 repeat the same-direction movement and reverse movement for a plurality of times in the thickness direction of the microfluidic chip, and the sample to be tested in the cleaning cavity is fully scattered by utilizing the repulsive magnetic force; and a third step of: the second magnet 1001 controls the cleaned sample to be measured to move into the eluting cavity 10 through the second valve chamber 8, the second magnet 1001 and the second magnetic device 101 repeatedly move in the same direction and in the opposite direction in the thickness direction of the microfluidic chip, and the sample to be measured in the eluting cavity 10 is fully dispersed by using repulsive magnetic force. The electrode array 11 is electrified, and through electrowetting, the liquid drop of the sample to be detected eluted in the eluting cavity 10 moves to the amplifying cavity 31 through the liquid path cavity 30 for amplification reaction, and the fluorescent detection device 200 detects the sample in the amplifying cavity 31 after amplification reaction.
Preferably, in the second step, after the second magnet 1001 and the second magnetic device 101 repeat the same-direction movement and the reverse movement for multiple times in the thickness direction of the microfluidic chip, the first magnet 1000 is moved to the position right below the cleaning cavity for primary enrichment, the first magnet 1000 is withdrawn, and then the second magnet 1001 is used for secondary enrichment of the sample to be tested in the cleaning cavity.
In summary, the microfluidic chip of the scheme is convenient to manufacture, so that the extraction efficiency is greatly improved, and the detection progress is effectively accelerated.
It should be noted that the foregoing is only a preferred embodiment of the present utility model, but the design concept of the present utility model is not limited thereto, and any insubstantial modifications made to the present utility model by using the concept fall within the scope of the present utility model.
Claims (7)
1. The microfluidic chip comprises a cracking cavity, a first valve chamber, a cleaning cavity, a second valve chamber, an elution cavity, a liquid path cavity and an amplification cavity which are sequentially communicated, wherein micro valves are arranged in the first valve chamber and the second valve chamber;
the microfluidic chip further comprises a chip substrate, wherein an electric connection port, an amplification region heating wire and an amplification region thermistor are arranged on the chip substrate, the amplification region heating wire is positioned right below the amplification cavity and is electrically connected with the electric connection port, and the amplification region heating wire is close to the amplification cavity relative to the amplification region thermistor;
the method is characterized in that:
the chip substrate is internally provided with a cracking zone heating wire, a cracking zone thermistor, a cleaning zone heating wire, a cleaning zone thermistor, an elution zone heating wire and an elution zone thermistor, wherein the cracking zone heating wire, the cracking zone thermistor, the cleaning zone heating wire, the cleaning zone thermistor, the elution zone heating wire and the elution zone thermistor are all electrically connected with the electric connection port;
the cracking zone heating wire and the cracking zone thermistor are both positioned right below the cracking cavity, and the cracking zone heating wire is close to the cracking cavity relative to the cracking zone thermistor;
the cleaning zone heating wire and the cleaning zone thermistor are both positioned right below the cleaning cavity, and the cleaning zone heating wire is close to the cleaning cavity relative to the cleaning zone thermistor;
the elution zone heating wire and the elution zone thermistor are both positioned right below the elution cavity and the liquid path cavity, and the elution zone heating wire is close to the elution cavity relative to the elution zone thermistor.
2. The microfluidic chip of claim 1, wherein:
the microfluidic chip comprises an extraction cover plate, and the extraction cover plate is arranged on the chip substrate;
a cracking groove with an opening at the bottom, a first valve groove with an opening at the bottom, a cleaning groove with an opening at the bottom and a second valve groove with an opening at the bottom which are communicated in sequence are arranged on one side of the extraction cover plate adjacent to the chip substrate;
a first sample adding hole, a second sample adding hole and an air hole are formed in one side, away from the chip substrate, of the extraction cover plate, the first sample adding hole is communicated with the cracking groove, the second sample adding hole is communicated with the cleaning groove, and the air hole is positioned between the cleaning groove and the second valve groove and is communicated with the cleaning groove and the second valve groove;
the microfluidic chip further comprises a sealing layer, the sealing layer is arranged between the extraction cover plate and the chip substrate, the sealing layer covers the bottom opening of the cracking groove to form the cracking cavity, the sealing layer covers the bottom opening of the first valve groove to form the first valve chamber, the sealing layer covers the bottom opening of the cleaning groove to form the cleaning cavity, and the sealing layer covers the bottom opening of the second valve groove to form the second valve chamber.
3. The microfluidic chip of claim 2, wherein:
the extraction cover plate is characterized in that at least one glue overflow groove is further formed in one side, adjacent to the chip substrate, of the extraction cover plate, one glue overflow groove is arranged on the outer sides of the cracking groove, the first valve groove, the cleaning groove and the second valve groove in a surrounding mode, and gaps are formed between the glue overflow groove and the cracking groove, between the glue overflow groove and the first valve groove, between the glue overflow groove and the second valve groove.
4. A microfluidic chip according to claim 3, wherein:
the microfluidic chip further comprises a conductive cover plate and a blocking layer, wherein the conductive cover plate is arranged on the chip substrate and is adjacent to the extraction cover plate, the blocking layer is positioned between the conductive cover plate and the chip substrate, and the elution cavity, the liquid path cavity and the amplification cavity are enclosed among the conductive cover plate, the blocking layer and the chip substrate;
the chip substrate further comprises an electrode array electrically connected with the electric connection port, the electrode array is located under the elution cavity, the liquid path cavity and the amplification cavity, and the electrode array is close to the conductive cover plate relative to the elution area heating wire and the amplification area heating wire.
5. The microfluidic chip according to any one of claims 2 to 4, wherein:
the micro valve comprises paraffin oil, a first paraffin valve heating wire, a first thermistor, a second paraffin valve heating wire and a second thermistor are further arranged on the chip substrate, and the first paraffin valve heating wire, the first thermistor, the second thermistor and the second paraffin valve heating wire are all electrically connected with the electrical connection port;
the first paraffin valve heating wire is positioned right below the first valve chamber, the first paraffin valve heating wire is close to the first valve chamber relative to the first thermistor, the second paraffin valve heating wire is positioned right below the second valve chamber, and the second paraffin valve heating wire is close to the second valve chamber relative to the second thermistor;
the extraction cover plate is provided with a third sample adding hole and a fourth sample adding hole on one side deviating from the chip substrate, the third sample adding hole is communicated with the first valve chamber, and the fourth sample adding hole is communicated with the second valve chamber.
6. The microfluidic chip according to any one of claims 2 to 4, wherein:
the number of the cleaning cavities can be more than two, and the second sample adding holes which are the same as the number of the cleaning cavities and are in one-to-one correspondence are arranged on the extraction cover plate.
7. Microfluidic system, including first magnetic means and second magnetic means, its characterized in that:
a microfluidic chip comprising any one of the above claims 1 to 6;
the first magnetic device is positioned right below the microfluidic chip, and the second magnetic device is positioned right above the microfluidic chip;
the first magnetic device comprises a first magnet and a second magnet, and the diameter of the first magnet is larger than that of the second magnet;
the first magnetic device and the second magnetic device can move along the length direction, the width direction and the thickness direction of the microfluidic chip;
the second magnet controls the sample to be tested with the magnetic beads to move from the cracking cavity to the eluting cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223125349.7U CN219886036U (en) | 2022-11-23 | 2022-11-23 | Microfluidic chip and microfluidic system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223125349.7U CN219886036U (en) | 2022-11-23 | 2022-11-23 | Microfluidic chip and microfluidic system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219886036U true CN219886036U (en) | 2023-10-24 |
Family
ID=88406416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223125349.7U Active CN219886036U (en) | 2022-11-23 | 2022-11-23 | Microfluidic chip and microfluidic system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219886036U (en) |
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2022
- 2022-11-23 CN CN202223125349.7U patent/CN219886036U/en active Active
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