CN219861381U

CN219861381U - Animal cell suspension culture bottle

Info

Publication number: CN219861381U
Application number: CN202321120683.5U
Authority: CN
Inventors: 史克昱; 郑珊珊; 樊锡君
Original assignee: Shanghai Beijin Biotechnology Co ltd
Current assignee: Shanghai Beijin Biotechnology Co ltd
Priority date: 2023-05-10
Filing date: 2023-05-10
Publication date: 2023-10-20
Anticipated expiration: 2033-05-10

Abstract

The utility model relates to an animal cell suspension culture bottle which comprises a culture bottle body, wherein the upper end of the culture bottle body is provided with a bottle stopper, the lower end face of the bottle stopper is fixedly provided with a first conical surface, the inner wall of the culture bottle body is provided with a second conical surface which is positioned at a bottle opening, the first conical surface is positioned in the second conical surface, two side faces of the culture bottle body are fixedly provided with rotating shafts which are positioned at the bottle opening, the rotating shafts are rotatably provided with connecting plates, the other ends of the connecting plates are fixedly provided with top plates, the upper end faces of the top plates are provided with threaded holes, the interiors of the threaded holes are provided with threaded columns, the lower ends of the threaded columns are fixedly provided with pressing blocks, the upper end faces of the bottle stopper are provided with grooves, and the pressing blocks are positioned in the grooves, so that a user can enable the pressing blocks to enter the grooves through rotating a hand wheel to apply downward pressure to the bottle stopper, and further enable the first conical surface to enter the interiors of the second conical surface, the tightness between the bottle stopper and the culture bottle is improved, and external impurities are prevented from entering.

Description

Animal cell suspension culture bottle

Technical Field

The utility model relates to the technical field of cell culture, in particular to an animal cell suspension culture bottle.

Background

Animal cell culture is one of the common techniques of animal cell engineering, and is also the basis of animal cell engineering, animal cell culture is the technique of taking out relevant tissues from an animal, dispersing the tissues into single cells, then putting the single cells under proper culture conditions, allowing the cells to grow and proliferate, and through verification, some types of cells can grow in a simple culture bottle, and by the period of 20 th 60 s, a pilot reactor with hundreds of liters is operated, and the method can be used for suspension culture of cells which are most common in mass production and industrial use, and can be divided into protein production cells, vaccine production cells and protein transient expression cell lines according to products, but the joint of a bottle plug at the upper end of a cell suspension culture bottle in the prior art and a bottle body does not have a sealing structure, so that external impurities easily enter the interior of the bottle body, and the normal culture environment of the cells is affected; and the cells need to be filled with sterile gas during culture, the gas can generate bubbles on the surface of the suspension, and the cells are damaged by the blasting force generated during bubble blasting due to different bubble sizes.

Disclosure of Invention

(one) solving the technical problems

Aiming at the problems in the prior art, the utility model provides an animal cell suspension culture bottle for solving the technical problems in the background art.

(II) technical scheme

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides an animal cell suspension blake bottle, includes the blake bottle, the upper end of blake bottle is equipped with the bottle plug, the fixed first conical surface that is equipped with of lower terminal surface of bottle plug, the second conical surface has been seted up on the inner wall of blake bottle and is located bottleneck department, first conical surface is located the inside of second conical surface, both sides face and the bottleneck department that is located of blake bottle all are fixed and are equipped with the pivot, all rotate in the pivot and be equipped with the connecting plate, the other end of connecting plate is all fixed and is equipped with the roof, the screw hole has been seted up to the up end of roof, the inside of screw hole is equipped with the screw thread post, the lower extreme of screw thread post is fixed and is equipped with the briquetting, the up end of bottle plug has been seted up flutedly, the briquetting is located the inside of recess, the upper end of screw thread post passes the roof and is fixed and is equipped with the hand wheel, be equipped with the fixed plate on the outer wall of blake bottle, the up end of fixed plate is equipped with the gas jar.

Preferably, the up end of gas holder is fixed and is equipped with the intake pipe, the other end and the blake bottle intercommunication of intake pipe, on the inner wall of blake bottle and be located the lower extreme fixedly and be equipped with broken net, the other end of intake pipe passes broken net and extends to the bottom of blake bottle, be equipped with the air pump in the intake pipe, be convenient for make gas enter into in the blake bottle.

In a further preferred aspect, the lower end face of the culture flask is fixedly provided with a first ear plate, the first ear plates are provided with a plurality of circumferentially arranged ear plates, the lower end of the culture flask is provided with a shell, the upper end face of the shell is correspondingly provided with a second ear plate, and the first ear plates and the second ear plates are fixedly mounted by bolts, so that the shell is convenient to detach.

In a further preferred embodiment, a heating pipe is disposed in the casing, and a plug is disposed at an outer end of the casing, and the plug is electrically connected with the heating pipe, so as to facilitate heating of the culture flask.

In a further preferred aspect, the first and second conical surfaces have equal inclination, so as to increase the tightness between the bottle stopper and the culture flask.

In a further preferred aspect, an anti-slip base is fixedly arranged on the lower end face of the shell, so that the mechanism is supported conveniently.

In a further preferred embodiment, a limiting block is fixedly arranged at one end of the rotating shaft, so that the limiting of the connecting plate is facilitated.

(III) beneficial effects

Compared with the prior art, the utility model provides an animal cell suspension culture bottle, which has the following beneficial effects:

1. according to the utility model, by arranging the culture bottle, the bottle plug, the first conical surface, the second conical surface, the rotating shaft, the connecting plate, the top plate, the threaded column, the pressing block, the groove and the hand wheel, a user can plug the bottle plug at the upper end of the culture bottle, then rotate the top plate to the upper end of the bottle plug through the cooperation of the rotating shaft and the connecting plate, then rotate the hand wheel to enable the pressing block to enter the groove so as to apply downward pressure to the bottle plug, and further enable the first conical surface to enter the second conical surface, so that the tightness between the bottle plug and the culture bottle is improved, and external impurities are prevented from entering to influence the culture environment of cells;

2. according to the utility model, the fixing plate, the gas storage tank, the gas inlet pipe, the crushing net and the gas pump are arranged, so that a user can open the gas pump to enable other culture gases such as oxygen in the gas storage tank to enter the culture bottle so as to provide gas for cells suspended on the culture solution, at the moment, the gas in the culture bottle can form large bubbles in the culture solution, and when the large bubbles rise in the culture solution, the large bubbles can contact with the crushing net and then are split into small bubbles, so that damage to the cells caused by blasting of the large bubbles is prevented, and the use is convenient;

3. according to the utility model, the first ear plate, the shell, the second ear plate, the heating pipe and the plug are arranged, so that a user can connect the heating pipe with an external power supply through the plug to heat the bottom of the culture bottle, and further the culture solution is in a relatively comfortable environment, so that the cells are accelerated to reproduce.

Drawings

FIG. 1 is a schematic diagram showing the overall structure of an animal cell suspension flask according to the present utility model;

FIG. 2 is a schematic view of the positions of a heating tube and a plug on a housing according to the present utility model;

FIG. 3 is a sectional view showing the installation of the flask, stopper and air inlet tube of the present utility model;

FIG. 4 is a schematic view showing the positions of a rotating shaft and a limiting block on a culture flask;

FIG. 5 is a schematic view of the installation of the connecting plate, top plate, threaded post, press block and hand wheel of the present utility model.

In the figure: 1. a culture bottle; 2. a bottle stopper; 3. a first conical surface; 4. a second conical surface; 5. a rotating shaft; 6. a connecting plate; 7. a top plate; 8. a threaded hole; 9. a threaded column; 10. briquetting; 11. a groove; 12. a hand wheel; 13. a fixing plate; 14. a gas storage tank; 15. an air inlet pipe; 16. crushing the net; 17. an air pump; 18. a first ear plate; 19. a housing; 20. a second ear plate; 21. heating pipes; 22. a plug; 23. an anti-slip base; 24. and a limiting block.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Example 1:

referring to fig. 1-5, an animal cell suspension culture bottle comprises a culture bottle 1, wherein a bottle stopper 2 is arranged at the upper end of the culture bottle 1, a first conical surface 3 is fixedly arranged on the lower end face of the bottle stopper 2, a second conical surface 4 is arranged on the inner wall of the culture bottle 1 and at the bottle opening, the first conical surface 3 is positioned inside the second conical surface 4, rotating shafts 5 are fixedly arranged at the two side faces and the bottle opening of the culture bottle 1, connecting plates 6 are rotatably arranged on the rotating shafts 5, a top plate 7 is fixedly arranged at the other end of each connecting plate 6, a threaded hole 8 is formed in the upper end face of each top plate 7, a threaded column 9 is arranged in the threaded hole 8, a pressing block 10 is fixedly arranged at the lower end of each threaded column 9, a groove 11 is formed in the upper end face of each bottle stopper 2, each pressing block 10 is positioned in the groove 11, the upper end of each threaded column 9 penetrates through the top plate 7 and is fixedly provided with a hand wheel 12, a fixing plate 13 is arranged on the outer wall of the culture bottle 1, and a gas storage tank 14 is arranged on the upper end face of each fixing plate 13.

In this embodiment, the air inlet pipe 15 is fixedly arranged on the upper end surface of the air storage tank 14, the other end of the air inlet pipe 15 is communicated with the culture flask 1, the crushing net 16 is fixedly arranged on the inner wall of the culture flask 1 and positioned at the lower end, the other end of the air inlet pipe 15 penetrates through the crushing net 16 and extends to the bottom of the culture flask 1, the air pump 17 is arranged on the air inlet pipe 15, and a user can enable gas in the air storage tank 14 to enter the culture flask 1 by opening the air pump 17 so as to provide gas for cell culture.

In this embodiment, the lower end surface of the culture flask 1 is fixedly provided with a first ear plate 18, the first ear plates 18 are provided with a plurality of circumferentially arranged, the lower end of the culture flask 1 is provided with a housing 19, the upper end surface of the housing 19 is correspondingly provided with a second ear plate 20, the first ear plate 18 and the second ear plate 20 are fixedly mounted by bolts, and a user can detach the housing 19 by bolts.

In the present embodiment, the heating tube 21 is disposed inside the housing 19, the plug 22 is disposed at the outer end of the housing 19, the plug 22 is electrically connected with the heating tube 21, and a user can connect with an external power source through the plug 22 to heat the heating tube 21.

In this embodiment, the first conical surface 3 and the second conical surface 4 have the same inclination, so that the sealing performance between the bottle stopper 2 and the culture bottle 1 can be improved.

In this embodiment, the lower end surface of the housing 19 is fixedly provided with an anti-slip base 23, so that the mechanism can be more stable.

In this embodiment, a limiting block 24 is fixedly disposed at one end of the rotating shaft 5, and can limit one side of the connecting plate 6.

Example 2:

in summary, when using, the user can be with bottle plug 2 to the upper end of blake bottle 1 earlier, then rethread pivot 5 and connecting plate 6's cooperation is come with roof 7 rotation to the upper end of bottle plug 2, afterwards, rethread rotates hand wheel 12 and makes briquetting 10 enter into the inside of recess 11 in order to apply decurrent pressure to bottle plug 2, and then make first conical surface 3 enter into the inside of second conical surface 4, increase the leakproofness between bottle plug 2 and the blake bottle 1, prevent external impurity entering, influence the culture environment of cell, and the cell in suspension in blake bottle 1 is in the middle of the blake bottle 1 in order to provide the nourishment for the cell in suspension on the blake liquid through opening air pump 17 messenger's oxygen among the gas jar 14, at this moment blake bottle 1 gaseous just can form big bubble in the middle of the blake liquid, when big bubble rises in the middle of the blake liquid, can contact with broken net 16, and then be split into the microbubble, cause the damage to the cell when preventing big bubble, make things convenient for cell absorption, and can also be in addition through the user 21 in order to make the bottom of the heat up the heat pipe 1 in order to make the relative air intake to the bottom of the blake bottle when being in addition, and the user is in order to make the bottom of the 20 is in order to be more than the environment.

In all the above mentioned solutions, in which the connection between two components can be chosen according to the actual situation, a welded, bolt-and-nut-fitted connection, a bolt-or-screw connection or other known connection means, which are not described in detail herein, where reference is made to a written fixed connection, the preferred consideration is welding, although embodiments of the utility model have been shown and described, it will be understood by those skilled in the art that numerous variations, modifications, substitutions and alterations can be made to these embodiments without departing from the principle and spirit of the utility model, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An animal cell suspension culture bottle, includes blake bottle (1), its characterized in that: the upper end of blake bottle (1) is equipped with bottle plug (2), the lower terminal surface of bottle plug (2) is fixed to be equipped with first conical surface (3), second conical surface (4) are seted up on the inner wall of blake bottle (1) and are located bottleneck department, first conical surface (3) are located the inside of second conical surface (4), both sides face and the bottleneck department of blake bottle (1) are all fixed and are equipped with pivot (5), all rotate on pivot (5) and are equipped with connecting plate (6), the other end of connecting plate (6) is all fixed and is equipped with roof (7), threaded hole (8) are seted up to the up end of roof (7), the inside of threaded hole (8) is equipped with screw thread post (9), the lower extreme of screw thread post (9) is fixed and is equipped with briquetting (10), fluted (11) are seted up to the up end of bottle plug (2), briquetting (10) are located the inside of recess (11), the upper end of screw thread post (9) passes roof (7) and is fixed and is equipped with hand wheel (12), be equipped with fixed on the outer wall of blake bottle (1) fixed plate (13), be equipped with air pot (13).

2. An animal cell suspension culture flask according to claim 1, wherein: the utility model discloses a gas storage tank, including gas storage tank (14), gas inlet pipe (15) are fixed to the up end of gas storage tank (14), the other end and the blake bottle (1) intercommunication of gas inlet pipe (15), on the inner wall of blake bottle (1) and be located the fixed broken net (16) that are equipped with of lower extreme, the other end of gas inlet pipe (15) passes broken net (16) and extends to the bottom of blake bottle (1), be equipped with air pump (17) on gas inlet pipe (15).

3. An animal cell suspension culture flask according to claim 1, wherein: the utility model discloses a culture flask, including first otic placode (18) is fixed to the lower terminal surface of blake flask (1), first otic placode (18) are provided with a plurality of and become the circumference shape and arrange, the lower extreme of blake flask (1) is equipped with casing (19), the up end of casing (19) corresponds and is equipped with second otic placode (20), first otic placode (18) adopts bolt fixed mounting with second otic placode (20).

4. An animal cell suspension culture flask according to claim 3, wherein: the inside of casing (19) is equipped with heating pipe (21), the outer end of casing (19) is equipped with plug (22), plug (22) and heating pipe (21) adopt electric connection.

5. An animal cell suspension culture flask according to claim 1, wherein: the inclination of the first conical surface (3) is equal to that of the second conical surface (4).

6. An animal cell suspension culture flask according to claim 3, wherein: an anti-slip base (23) is fixedly arranged on the lower end face of the shell (19).

7. An animal cell suspension culture flask according to claim 1, wherein: one end of the rotating shaft (5) is fixedly provided with a limiting block (24).