CN219861371U - Novel magnetic head cap for separating target cells - Google Patents
Novel magnetic head cap for separating target cells Download PDFInfo
- Publication number
- CN219861371U CN219861371U CN202320359860.9U CN202320359860U CN219861371U CN 219861371 U CN219861371 U CN 219861371U CN 202320359860 U CN202320359860 U CN 202320359860U CN 219861371 U CN219861371 U CN 219861371U
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- Prior art keywords
- head cap
- magnetic head
- culture plate
- hole culture
- utility
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- 238000000926 separation method Methods 0.000 claims abstract description 20
- 238000004113 cell culture Methods 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 7
- 238000012423 maintenance Methods 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 description 36
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 238000007898 magnetic cell sorting Methods 0.000 description 4
- 238000007885 magnetic separation Methods 0.000 description 3
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000011325 microbead Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000002390 adhesive tape Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of cell separation tools, and particularly discloses a novel magnetic head cap for separating target cells, which comprises a six-hole culture plate for cell culture and a magnetic head cap arranged on the six-hole culture plate; the magnetic head cap comprises a shell for supporting, two convex columns which are arranged in a staggered mirror image mode are arranged at the bottom of the shell, and the convex columns are contacted with the inner sides of the culture holes of the six-hole culture plate; the utility model can directly form a certain distance between the bottom of the magnetic head cap and the six-hole culture plate by utilizing the combination of the magnetic head cap and the six-hole culture plate through the convex column which is arranged at the bottom of the magnetic head cap and protrudes downwards, and the magnetic head cap can be used for conveniently and better magnetically sorting cells to meet the use requirement, and meanwhile, the requirement of fixing the magnetic head cap in the six-hole culture plate is further improved.
Description
Technical Field
The utility model relates to the technical field of cell separation tools, in particular to a novel magnetic head cap for separating target cells.
Background
Among the means of cell separation, there are two methods currently in common use: flow cell sorting and magnetic cell sorting, but in whatever way. In the early stages, many physical separation methods were used to separate specific cell populations, such as target cells.
Regardless of the type of cells, the accuracy of separation cannot be guaranteed after the cells are physically separated before separation, and the cells to be collected need to be screened again;
the magnetic cell sorting mode can utilize nanometer iron-containing microbeads capable of recognizing and combining cell surface antigens, namely magnetic beads, which are commonly known, to be retained in a super-strong magnetic field separation column to realize the cell separation technology. The initial cell sample is marked aiming at the specificity of the initial cell sample, the marked cell sample is loaded and enters a magnetic field separation column for magnetic separation, the mode is very easy to be used by hands, and the number of the modes is relatively large;
however, when the magnetic separation is performed, the magnetic head cap (a common tool for magnetically separating cells) is used for contacting cells, the bottom of the magnetic head cap is contacted with the cell culture solution due to direct contact, so that the magnetic separation at the central position close to the bottom is obviously unreasonable, and based on the fact, a novel magnetic head cap for separating target cells is provided.
Disclosure of Invention
In view of the shortcomings of the prior art, the present utility model provides a novel magnetic head cap for separating target cells, which is used for solving the problems set forth in the background art.
The novel magnetic head cap for separating target cells comprises a six-hole culture plate for cell culture and a magnetic head cap arranged on the six-hole culture plate;
the magnetic head cap comprises a shell for supporting, two protruding columns which are arranged in a staggered mirror image mode are arranged at the bottom of the shell, and the protruding columns are in contact with the inner sides of culture holes of the six-hole culture plate.
As a further improvement of the present utility model, the top of the head cap is provided with a panel having an area larger than that of the top of the head cap housing.
As a further improvement of the utility model, two sides of the shell are provided with vertical sliding rails, and the inner sides of the sliding rails are connected with auxiliary sliding frames in a sliding manner.
As a further improvement of the utility model, a limit groove is arranged on one side of the auxiliary sliding frame, which is close to the convex column, and a maintenance part capable of assisting the stability of the magnetic head cap is filled in the limit groove.
As a further improvement of the utility model, a sliding block is integrally formed on one side of the auxiliary sliding frame, which is close to the sliding rail, and the sliding block is connected with the inner side of the sliding rail in a sliding way.
As a further improvement of the utility model, the bottom of the auxiliary slide frame is contacted with the top of the six-hole culture plate.
Compared with the prior art, the utility model has the following beneficial effects:
according to the utility model, through the downward protruding column at the bottom of the inner box of the magnetic head cap, the bottom of the magnetic head cap and the six-hole culture plate can be directly formed into a certain distance by utilizing the combination of the magnetic head cap and the six-hole culture plate, the magnetic head cap can be conveniently used for carrying out magnetic sorting on cells, the use requirement is met, meanwhile, in order to further improve the requirement of fixing the magnetic head cap in the six-hole culture plate, two vertical sliding rails are arranged at the outer side of the magnetic head cap shell, and the sliding rails are utilized to cooperate with the auxiliary sliding frame, so that the maintenance and stability component arranged at the bottom of the auxiliary sliding frame can be better combined with the six-hole culture plate when being matched with the six-hole culture plate, thereby limiting the magnetic head cap, improving the stability of the magnetic head cap, avoiding influencing the culture solution in the six-hole culture plate in the whole use process, and better meeting the use requirement.
Drawings
The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the utility model and do not constitute a limitation on the utility model. In the drawings:
FIG. 1 is a schematic diagram of a six-well plate and head cap according to the present utility model;
FIG. 2 is a schematic perspective view of a magnetic head cap according to the present utility model;
FIG. 3 is a schematic view of another perspective view of a magnetic head cap according to the present utility model;
FIG. 4 is a schematic view of the bottom structure of the magnetic head cap of the present utility model;
FIG. 5 is a schematic view of the cross-sectional structure A-A of FIG. 4 according to the present utility model.
In the figure: 1. six-hole culture plate; 2. a head cap; 21. a housing; 22. an auxiliary sliding frame; 23. a top panel; 24. a slide rail; 25. a convex column; 26. and a limit groove.
Detailed Description
Various embodiments of the present utility model are disclosed in the following drawings, which are presented in sufficient detail to provide a thorough understanding of the present utility model. However, it should be understood that these physical details should not be used to limit the utility model. That is, in some embodiments of the present utility model, these physical details are not necessary. Moreover, for the sake of simplicity of illustration, some well-known and conventional structures and components are shown in the drawings in a simplified schematic manner.
In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present utility model.
Referring to fig. 1 and 2, there are two methods commonly used in cell sorting: the method comprises the steps of carrying out flow cell sorting and magnetic cell sorting, but separating cells in the earlier stage in a physical way, processing specific cell groups, screening by matching with the two ways, and accurately extracting the required cells, for example, in the process of separating target cells, processing the cells in a magnetic cell sorting way generally commonly, wherein the adopted tool is a magnetic head cap 2, the magnetic head cap 2 is contacted with culture solution in a culture plate, nano iron-containing microbeads capable of recognizing and combining cell surface antigens are utilized, the technology of realizing cell separation is a specific mark separation way of the cells in a super-strong magnetic field separation column, and finally, the whole cell sorting process can be completed through elution, the upper hand is relatively simple, the bottom of the magnetic head cap 2 is pressed near the central position of the bottom of the magnetic head cap 2 after the bottom of the magnetic head cap 2 is contacted with the culture solution, the bottom of the magnetic head cap 2 is flat and forms the bottom near the central position, the magnetic head cap 2 is not reasonably influenced, and the magnetic head cap 2 is used for providing a novel magnetic head cap 1, which is used for the cell separation of the culture plate, and the novel magnetic head cap 1 is used for the cell separation of the six-based on the culture plate, and the six-type-cell separation plate is provided;
specifically, the magnetic head cap 2 comprises a shell 21 for supporting, two convex columns 25 which are arranged in a staggered mirror image are arranged at the bottom of the shell 21, and the convex columns 25 are contacted with the inner sides of the culture holes of the six-hole culture plate 1;
in this embodiment, the protruding column 25 arranged at the bottom of the magnetic head cap 2 is matched with the culture holes in the six-hole culture plate 1, under the lifting action of the protruding column 25, the bottom of the magnetic head cap 2 is suspended in the culture solution, the magnetic head cap 2 can not directly press the culture solution to the bottom of the culture plate under the action of gravity, the cell sorting effect can be improved, and the requirement of later use can be met;
referring to fig. 1, 2 and 3, after the cell sorting is completed, in order to conveniently remove the head cap 2 from the culture plate, a panel 23 may be disposed on the top of the head cap 2, and the area of the panel 23 is larger than the top area of the housing 21 of the head cap 2.
In the above embodiment, the panel 23 disposed on the top of the head cap 2 has an area larger than the top area of the housing 21 of the head cap 2, so that after the post screening is completed, the whole head cap 2 can be conveniently taken out through the panel 23, and the next operation step can be performed;
referring to fig. 2, 3, 4 and 5, in order to facilitate the stable use of the magnetic head cap 2 in culturing the six-hole culture plate 1 during the normal operation of the magnetic head cap 2, the present utility model can avoid the problem of shaking the magnetic head cap 2 due to the upper center of gravity, and according to the present utility model, vertical sliding rails 24 are provided on both sides of the housing 21, and an auxiliary sliding frame 22 is slidingly connected to the inner side of the sliding rail 24.
Further, a limit groove 26 is provided on one side of the auxiliary sliding frame 22 near the protruding column 25, and a maintenance component capable of assisting the stability of the magnetic head cap 2 is filled in the limit groove 26.
Further, a slider is integrally formed on one side of the auxiliary sliding frame 22 adjacent to the sliding rail 24, and the slider is slidably connected to the inner side of the sliding rail 24.
At the same time, the bottom of the auxiliary slide 22 is in contact with the top of the six-well culture plate 1.
In this embodiment, the sliding rails 24 disposed on two sides of the housing 21 are matched with the auxiliary sliding frame 22, so that when the magnetic head cap 2 is used in normal operation, the auxiliary sliding frame 22 is located at the top of the six-hole culture plate 1 and moves downwards, and the stability of the magnetic head cap 2 can be maintained through the use of the maintenance component;
the above-mentioned used maintenance component is a device formed by one or more components for keeping stand column stable, such as a negative pressure sucker, an adhesive tape, etc., which are commonly used in the mechanical field, and is generally preferably a negative pressure sucker from common use modes, and by means of pressing downwards, the sucker can be contacted with the edge of the culture hole at the top of the six-hole culture plate 1, and by means of the contact, the magnetic head cap 2 can be kept fixed, so as to meet the use requirement.
The foregoing description is only illustrative of the utility model and is not to be construed as limiting the utility model. Various modifications and variations of the present utility model will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, or the like, which is within the spirit and principle of the present utility model, should be included in the scope of the claims of the present utility model.
Claims (6)
1. A novel magnetic head cap for separating target cells comprises a six-hole culture plate (1) for cell culture and a magnetic head cap (2) arranged on the six-hole culture plate (1);
the method is characterized in that:
the magnetic head cap (2) comprises a shell (21) for supporting, two protruding columns (25) which are arranged in a staggered mirror image mode are arranged at the bottom of the shell (21), and the protruding columns (25) are in contact with the inner sides of culture holes of the six-hole culture plate (1).
2. A novel magnetic head cap for target cell separation according to claim 1, wherein: the top of the magnetic head cap (2) is provided with a panel (23), and the area of the panel (23) is larger than the top area of the shell (21) of the magnetic head cap (2).
3. A novel magnetic head cap for target cell separation according to claim 1, wherein: both sides of the shell (21) are provided with vertical sliding rails (24), and the inner sides of the sliding rails (24) are connected with auxiliary sliding frames (22) in a sliding manner.
4. A novel magnetic head cap for target cell separation according to claim 3, wherein: a limit groove (26) is formed in one side, close to the convex column (25), of the auxiliary sliding frame (22), and a maintenance part capable of assisting the magnetic head cap (2) to be stable is filled in the limit groove (26).
5. A novel magnetic head cap for target cell separation according to claim 3, wherein: one side of the auxiliary sliding frame (22) close to the sliding rail (24) is integrally formed with a sliding block, and the sliding block is connected with the inner side of the sliding rail (24) in a sliding manner.
6. A novel magnetic head cap for target cell separation according to claim 3, wherein: the bottom of the auxiliary sliding frame (22) is contacted with the top of the six-hole culture plate (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320359860.9U CN219861371U (en) | 2023-02-28 | 2023-02-28 | Novel magnetic head cap for separating target cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320359860.9U CN219861371U (en) | 2023-02-28 | 2023-02-28 | Novel magnetic head cap for separating target cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219861371U true CN219861371U (en) | 2023-10-20 |
Family
ID=88339369
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320359860.9U Active CN219861371U (en) | 2023-02-28 | 2023-02-28 | Novel magnetic head cap for separating target cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219861371U (en) |
-
2023
- 2023-02-28 CN CN202320359860.9U patent/CN219861371U/en active Active
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