CN219839698U - Umbilical cord mesenchymal stem cell purification equipment - Google Patents
Umbilical cord mesenchymal stem cell purification equipment Download PDFInfo
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- CN219839698U CN219839698U CN202321124849.0U CN202321124849U CN219839698U CN 219839698 U CN219839698 U CN 219839698U CN 202321124849 U CN202321124849 U CN 202321124849U CN 219839698 U CN219839698 U CN 219839698U
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- Prior art keywords
- mesenchymal stem
- barrel
- umbilical cord
- cord mesenchymal
- barrel body
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- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 39
- 210000003954 umbilical cord Anatomy 0.000 title claims abstract description 35
- 238000000746 purification Methods 0.000 title claims abstract description 27
- 238000007789 sealing Methods 0.000 claims abstract description 18
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000004744 fabric Substances 0.000 claims abstract description 14
- 239000002808 molecular sieve Substances 0.000 claims abstract description 14
- 238000004587 chromatography analysis Methods 0.000 claims description 11
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 239000008280 blood Substances 0.000 abstract description 19
- 210000004369 blood Anatomy 0.000 abstract description 19
- 238000001914 filtration Methods 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 description 7
- 210000000130 stem cell Anatomy 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
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- Centrifugal Separators (AREA)
Abstract
The utility model discloses umbilical cord mesenchymal stem cell purification equipment, which comprises a purification piece, wherein the purification piece comprises a barrel body, the top end of the barrel body is provided with an opening, the middle part of the bottom surface of the barrel body is upwards provided with a convex shell, the top end of the shell inside the barrel body is rotationally connected with a centrifugal barrel, the top of the shell outside the barrel body is fixedly provided with a motor, the output end of the motor is connected with the bottom end of the centrifugal barrel, the top surface of the centrifugal barrel is provided with an opening, and the outer circumferential surface of the centrifugal barrel consists of a molecular sieve and a plurality of layers of chromatographic fabrics; the sealing element comprises a sealing cover, the sealing cover is assembled on the opening end face of the barrel body through threads, and the middle part of the sealing cover is vertically penetrated and fixed with a pressurizing element. The utility model overcomes the problems that the time for the umbilical cord mesenchymal stem cells in the blood to pass through the filtering device is longer and the purifying efficiency of the umbilical cord mesenchymal stem cells is lower because the purification of the umbilical cord mesenchymal stem cells in the prior art lacks the assistance of external force to accelerate the umbilical cord mesenchymal stem cells in the blood to pass through the filtering device.
Description
Technical Field
The utility model relates to the technical field of stem cell purification devices, in particular to umbilical cord mesenchymal stem cell purification equipment.
Background
The umbilical cord mesenchymal stem cells are used as reagents for in-vitro diagnosis, medicines for treating diseases, ligands for immunoaffinity chromatography and the like, the umbilical cord mesenchymal stem cells are used as core reagents for various immunoassays, the sensitivity and the specificity of immunoassay results play a vital role, in the preparation of the existing umbilical cord mesenchymal stem cells, the extracted stem cell primary pulp has other impurities, the purification is needed for the subsequent culture work, the existing extracted umbilical cord mesenchymal stem cells mostly use a filter device composed of a molecular sieve and a multi-layer chromatography fabric, blood is settled downwards in the filter device under the action of self gravity, and the stem cells flow downwards into a collecting cylinder through the filter device to filter and purify the stem cells in the blood.
However, the above purification of umbilical cord mesenchymal stem cells relies on the fact that blood is settled downwards under the action of self gravity to pass through a filter device formed by a molecular sieve and a multi-layer chromatography fabric, and the umbilical cord mesenchymal stem cells in the blood are accelerated to pass through the filter device due to the lack of the assistance of external force, so that the time for the umbilical cord mesenchymal stem cells in the blood to pass through the filter device is long, and the purification efficiency of the umbilical cord mesenchymal stem cells is low.
Disclosure of Invention
The utility model aims to provide umbilical cord mesenchymal stem cell purification equipment, which aims to solve the problems.
The utility model is realized in the following way: an umbilical cord mesenchymal stem cell purification apparatus, comprising:
the purifying piece comprises a barrel body, the top end opening of the barrel body is arranged, the middle part of the bottom surface of the barrel body is upwards provided with a convex shell, the top end of the shell inside the barrel body is rotationally connected with a centrifugal barrel, the top of the shell outside the barrel body is fixedly provided with a motor, the output end of the motor is connected with the bottom end of the centrifugal barrel, the top surface opening of the centrifugal barrel is arranged, and the outer circumferential surface of the centrifugal barrel consists of a molecular sieve and a plurality of layers of chromatographic fabrics;
the sealing piece comprises a sealing cover, the sealing cover is assembled on the opening end face of the barrel body through threads, and the middle part of the sealing cover is vertically penetrated and fixed with a pressurizing piece.
Further, the pressurizing piece comprises an air inlet pipe, the air inlet pipe is vertically communicated, inserted and fixed on the sealing cover, and the upper part of the air inlet pipe is communicated with an air pump.
Further, one end of the air inlet pipe extending to the inside of the barrel body is assembled and communicated with a guide pipe through threads, and the bottom end of the guide pipe is fixedly provided with a plastic cover in a penetrating manner.
Further, the bottom of pipe is provided with elasticity gasbag, and the inlet end intercommunication of elasticity gasbag is fixed in the bottom of pipe.
Further, the below of staving is provided with support piece, and support piece includes the backing ring board, and the backing ring board level sets up in the below of staving, and is fixed with many springs on the top surface of backing ring board vertically, and the top of many springs is fixed on the bottom surface of staving.
Further, a vibration motor is arranged on the barrel body.
Further, a liquid discharge pipe is vertically communicated with the bottom surface of the barrel body, and a valve is arranged on the liquid discharge pipe.
Compared with the prior art, the utility model has the beneficial effects that: the use is added the blood that contains umbilical cord mesenchymal stem cells in the centrifuge bowl of purification spare barrel, then will, the shrouding on the sealing member seals in the open end of staving, switch on the closed motor power supply way, the motor starts the casing top rotation of drive centrifuge bowl in the staving inside and produces centrifugal force, make umbilical cord mesenchymal stem cells in the blood get into in the staving through molecular sieve and multilayer chromatography surface fabric filtration, then discharge through the fluid-discharge tube and collect, thereby accelerate umbilical cord mesenchymal stem cells in the blood under the effect of centrifugal force in the centrifuge bowl and filter through molecular sieve and multilayer chromatography surface fabric, the efficiency of umbilical cord mesenchymal stem cells in the purification blood has been improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view of the overall structure of the present utility model;
FIG. 2 is a schematic exploded view of the present utility model;
FIG. 3 is a schematic view showing the structure of a purification member in an exploded state according to an embodiment of the present utility model;
FIG. 4 is a schematic view of the seal in an exploded configuration in accordance with an embodiment of the present utility model;
fig. 5 is a schematic view showing a structure of the support member in an exploded state in the embodiment of the present utility model.
In the figure: 1. a purifying member; 11. a tub body; 12. a centrifugal barrel; 13. a motor; 14. a chromatographic fabric; 15. a molecular sieve; 16. a liquid discharge pipe; 17. a vibration motor; 2. a seal; 21. a cover; 22. a pressurizing member; 221. an air inlet pipe; 222. an air pump; 223. a conduit; 224. a plastic cover; 225. an elastic air bag; 3. a support; 31. a carrier ring plate; 32. and (3) a spring.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments. All other embodiments, based on the embodiments of the utility model, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the utility model. Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, based on the embodiments of the utility model, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the utility model.
Referring to fig. 1, 2, 3, 4 and 5, an umbilical cord mesenchymal stem cell purification apparatus comprises:
the purifying piece 1, the purifying piece 1 comprises a barrel 11, the top end opening of the barrel 11 is arranged, the middle of the bottom surface of the barrel 11 is upwards provided with a convex shell, the top end of the shell inside the barrel 11 is rotationally connected with a centrifugal barrel 12, the top of the shell outside the barrel 11 is fixedly provided with a motor 13 with an output end connected to the bottom end of the centrifugal barrel 12, the wiring end of the motor 13 is connected with an external power supply line through a wire series switch, the top surface of the centrifugal barrel 12 is arranged in an opening way, the outer circumferential surface of the centrifugal barrel 12 consists of a molecular sieve 15 and a multi-layer chromatographic fabric 14, the bottom surface of the barrel 11 is vertically communicated with a liquid discharge pipe 16, a valve is arranged on the liquid discharge pipe 16, and the liquid discharge pipe 16 is opened for discharging purified umbilical cord mesenchymal stem cells;
the sealing piece 2, the sealing piece 2 includes the closing cap 21, and closing cap 21 screw thread group is on the opening terminal surface of staving 11, the middle part vertical link up of closing cap 21 is fixed with the pressurizing piece 22, use the blood that will contain umbilical cord mesenchymal stem cells to add in the centrifuge bowl 12 of purification piece 1 barrel 11, then seal the shrouding 21 on the sealing piece 2 at the opening terminal of staving 11, switch on the power supply way of closed motor 13, motor 13 starts the casing top rotation that drives centrifuge bowl 12 in staving 11 inside and produces centrifugal force, make the umbilical cord mesenchymal stem cells in the blood filter through molecular sieve 15 and multilayer chromatography surface fabric 14 and get into staving 11, then discharge through fluid-discharge tube 16 and collect, thereby accelerate the umbilical cord mesenchymal stem cells in the blood through molecular sieve 15 and the filtration of multilayer chromatography surface fabric 14 under the effect of centrifugal force in centrifuge bowl 12, the efficiency of the umbilical cord mesenchymal stem cells in the purified blood has been improved.
Referring to fig. 4 and 5, the pressurizing member 22 includes an air inlet pipe 221, the air inlet pipe 221 is vertically inserted and fixed on the cover 21, the upper portion of the air inlet pipe 221 is communicated with an air pump 222, the terminal of the air pump 222 is connected with an external power supply line through a wire serial switch, one end of the air inlet pipe 221 extending to the inside of the barrel 11 is assembled and communicated with a conduit 223 through threads, the bottom end of the conduit 223 is fixedly provided with a plastic cover 224 in a penetrating manner, the bottom end of the conduit 223 is provided with an elastic air bag 225, and the air inlet end of the elastic air bag 225 is fixedly connected with the bottom end of the conduit 223.
Referring to fig. 3, a supporting member 3 is disposed below a barrel 11, the supporting member 3 includes a supporting ring plate 31, the supporting ring plate 31 is horizontally disposed below the barrel 11, a plurality of springs 32 are vertically fixed on the top surface of the supporting ring plate 31, the top ends of the springs 32 are fixed on the bottom surface of the barrel 11, a vibration motor 17 is mounted on the barrel 11, terminals of the vibration motor 17 are connected on an external power supply line through a wire series switch, in order to accelerate the filtration efficiency of umbilical cord mesenchymal stem cells in blood through a molecular sieve 15 and a multi-layer chromatography fabric 14, the switch on the power supply line of the vibration motor 17 is closed, the vibration motor 17 is started to vibrate to drive the barrel 11 on the purification member 1 to vibrate on the springs 32 of the supporting plate 31, and the umbilical cord mesenchymal stem cells in blood are accelerated to flow through the molecular sieve 15 and the filtration of the multi-layer chromatography fabric 14.
Working principle: in the use, the blood containing umbilical cord mesenchymal stem cells is added into the centrifugal barrel 12 of the barrel 11 of the purification piece 1, then the sealing plate 21 on the sealing piece 2 is sealed at the opening end of the barrel 11, the switch on the power supply circuit of the motor 13 is closed, the motor 13 is started to drive the centrifugal barrel 12 to rotate at the top end of the shell inside the barrel 11 to generate centrifugal force, so that the umbilical cord mesenchymal stem cells in the blood are filtered by the molecular sieve 15 and the multi-layer chromatography fabric 14 and enter the barrel 11, and then are discharged and collected by the liquid discharge pipe 16.
The above description is only of the preferred embodiments of the present utility model and is not intended to limit the present utility model, and various modifications and variations may be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (7)
1. An umbilical cord mesenchymal stem cell purification apparatus, comprising:
the purifying piece (1), purifying piece (1) includes staving (11), the open-ended setting of top of staving (11), and the bottom surface middle part of staving (11) upwards is provided with bellied casing, the inside casing top of staving (11) rotates and is connected with centrifugal barrel (12), and the outside casing top of staving (11) is fixed with motor (13) of output connection in centrifugal barrel (12) bottom, the open-ended setting of top of centrifugal barrel (12), and the outer periphery of centrifugal barrel (12) comprises molecular sieve (15) and multilayer chromatography surface fabric (14);
the sealing piece (2), the sealing piece (2) includes closing cap (21), and closing cap (21) screw thread group is on the opening terminal surface of staving (11), the middle part of closing cap (21) is vertical to be run through and is fixed with pressurization piece (22).
2. The umbilical cord mesenchymal stem cell purification device according to claim 1, wherein the pressurizing member (22) comprises an air inlet pipe (221), the air inlet pipe (221) is vertically and fixedly connected to the sealing cover (21) in a penetrating and inserting manner, and an air pump (222) is communicated with the upper part of the air inlet pipe (221).
3. An umbilical cord mesenchymal stem cell purification device according to claim 2, wherein one end of the air inlet pipe (221) extending into the barrel body (11) is in threaded assembly and communicated with a conduit (223), and the bottom end of the conduit (223) is fixedly penetrated by a plastic cover (224).
4. An umbilical mesenchymal stem cell purification device according to claim 3, wherein the bottom end of the conduit (223) is provided with an elastic balloon (225), and the air inlet end of the elastic balloon (225) is fixed at the bottom end of the conduit (223) in communication.
5. Umbilical mesenchymal stem cell purification equipment according to claim 1, wherein a supporting member (3) is arranged below the barrel body (11), the supporting member (3) comprises a supporting ring plate (31), the supporting ring plate (31) is horizontally arranged below the barrel body (11), a plurality of springs (32) are vertically fixed on the top surface of the supporting ring plate (31), and the top ends of the plurality of springs (32) are fixed on the bottom surface of the barrel body (11).
6. Umbilical mesenchymal stem cell purification equipment according to claim 5, characterized in that the barrel (11) is provided with a vibration motor (17).
7. The umbilical mesenchymal stem cell purification device according to claim 1, wherein a drain pipe (16) is vertically communicated with the bottom surface of the barrel body (11), and a valve is installed on the drain pipe (16).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321124849.0U CN219839698U (en) | 2023-05-11 | 2023-05-11 | Umbilical cord mesenchymal stem cell purification equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321124849.0U CN219839698U (en) | 2023-05-11 | 2023-05-11 | Umbilical cord mesenchymal stem cell purification equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219839698U true CN219839698U (en) | 2023-10-17 |
Family
ID=88297621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321124849.0U Active CN219839698U (en) | 2023-05-11 | 2023-05-11 | Umbilical cord mesenchymal stem cell purification equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219839698U (en) |
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2023
- 2023-05-11 CN CN202321124849.0U patent/CN219839698U/en active Active
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