CN219824179U - Serum-free culture medium - Google Patents
Serum-free culture medium Download PDFInfo
- Publication number
- CN219824179U CN219824179U CN202320916795.5U CN202320916795U CN219824179U CN 219824179 U CN219824179 U CN 219824179U CN 202320916795 U CN202320916795 U CN 202320916795U CN 219824179 U CN219824179 U CN 219824179U
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- China
- Prior art keywords
- racks
- serum
- rotate
- gear
- fixed shell
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- 239000004017 serum-free culture medium Substances 0.000 title abstract description 8
- 238000003825 pressing Methods 0.000 claims abstract description 22
- 238000007789 sealing Methods 0.000 claims abstract description 8
- 239000012679 serum free medium Substances 0.000 claims description 7
- 230000007246 mechanism Effects 0.000 claims description 6
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 abstract description 20
- 239000001963 growth medium Substances 0.000 description 19
- 239000000126 substance Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 230000004663 cell proliferation Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- -1 trace elements) Chemical class 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 241001446247 uncultured actinomycete Species 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a serum-free culture medium, which comprises a culture dish and a sealing cover, wherein the sealing cover is arranged on the upper side of the culture dish, a connecting ring is arranged at the upper end of the sealing cover, the sealing cover is in threaded sleeve joint with the connecting ring, a vertical rod is rotatably arranged in the middle of the lower surface of the sealing cover, and a plurality of stirring rods are fixedly sleeved on the rod wall of the vertical rod from top to bottom in sequence. According to the culture dish, the two pressing plates are pressed towards the direction of the fixed shell, so that the two pressing plates drive the corresponding racks to move, namely the gear is driven to rotate, the gear drives the vertical rods to rotate, so that the stirring rods are driven to rotate, then the springs push the two racks to reset, during the period, the two racks drive the gears to reversely rotate, namely the vertical rods are driven to reversely rotate, namely the stirring rods are driven to rotate again, namely the pressing plates on the two sides are repeatedly pressed, so that the vertical rods drive the stirring rods to repeatedly rotate and stir, and time-saving and labor-saving stirring can be carried out on culture mediums in the culture dish.
Description
Technical Field
The utility model relates to the technical field of culture medium filling, in particular to a serum-free culture medium.
Background
The culture medium refers to a nutrient medium which is used for the growth and reproduction of microorganisms, plants or animals (or tissues) and is prepared by combining different nutrient substances. Generally contains carbohydrate, nitrogenous substances, inorganic salts (including trace elements), vitamins, water and other substances. The culture medium is a basic substance for providing cell nutrition and promoting cell proliferation, and is also a living environment for cell growth and proliferation. The culture medium is of various types and can be classified into a natural culture medium, a synthetic culture medium and a semisynthetic culture medium according to the sources of the preparation raw materials; according to the physical state, it can be classified into solid medium, liquid medium, and semisolid medium; according to the culture function, the culture medium can be divided into basic culture medium, selective culture medium, enrichment culture medium, identification culture medium and the like; according to the application range, bacteria culture medium, actinomycete culture medium, yeast culture medium, fungi culture medium, etc. can be classified. After the culture medium is prepared, the pH is generally required to be tested and adjusted, and sterilization, such as high-temperature sterilization and filtration sterilization, is also required. The culture medium is easy to be polluted or deteriorated due to rich nutrient substances. It is not suitable for long-term placement after being prepared, and is best for being prepared at present.
At present, serum-free culture medium is easy to precipitate and delaminate after long-term storage, stirring is needed before use, stirring equipment is usually needed for stirring, extra carrying is needed, the operation process is complicated, and improvement is needed.
Disclosure of Invention
The utility model aims to provide a serum-free culture medium, which solves the problems in the background technology. Including culture dish and sealed lid, sealed lid sets up in the upside of culture dish, sealed upper end is provided with the go-between, sealed lid cup joints with the go-between screw thread, sealed lower surface middle part rotation of sealing lid is provided with the montant, the pole wall of montant has fixedly cup jointed a plurality of puddlers from last to down in proper order, sealed upper surface fixing of lid is provided with the fixed shell, the upper end of montant extends to the inside of fixed shell, the inside of fixed shell is provided with the drive montant pivoted drive mechanism.
As an alternative of the technical scheme of the utility model, the transmission mechanism comprises a gear and racks, the gear is arranged in the fixed shell, the gear is fixedly sleeved with the upper end of the vertical rod, the two racks are respectively meshed with the two sides of the gear, one ends of the two racks extend to the outer side of the fixed shell, a reset rod is movably sleeved in one inner end of the inner side of the two racks, one end of the reset rod is fixedly connected with the inner wall of the fixed shell, a spring is movably sleeved on the rod wall of the reset rod, and two ends of the spring are respectively fixedly connected with the corresponding racks and the inner wall of the fixed shell.
As an alternative of the technical scheme of the utility model, the longitudinal section of the reset rod is square.
As an alternative of the technical scheme of the utility model, two sides of the fixing shell are provided with pressing plates, and one ends of the outer sides of the two racks are fixedly connected with the corresponding pressing plates.
As an alternative of the technical scheme of the utility model, the middle ends of the two racks are fixedly sleeved with the baffle plates.
As an alternative of the technical scheme of the utility model, the outer walls of the two pressing plates are provided with anti-skid rubber pads.
Compared with the prior art, the utility model has the following beneficial effects:
according to the culture dish, the two pressing plates are pressed towards the direction of the fixed shell, so that the two pressing plates drive the corresponding racks to move, namely the gear is driven to rotate, the gear drives the vertical rods to rotate, so that the stirring rods are driven to rotate, then the springs push the two racks to reset, during the period, the two racks drive the gears to reversely rotate, namely the vertical rods are driven to reversely rotate, namely the stirring rods are driven to rotate again, namely the pressing plates on the two sides are repeatedly pressed, so that the vertical rods drive the stirring rods to repeatedly rotate and stir, and time-saving and labor-saving stirring can be carried out on culture mediums in the culture dish.
Drawings
Other features, objects and advantages of the present utility model will become more apparent upon reading of the detailed description of non-limiting embodiments, given with reference to the accompanying drawings in which:
FIG. 1 is a schematic diagram of a serum-free medium according to the present utility model;
fig. 2 is an enlarged schematic view of the structure of the portion a in fig. 1.
In the figure: 1. a culture dish; 2. sealing cover; 3. a connecting ring; 4. a vertical rod; 5. a stirring rod; 6. a fixed case; 7. a gear; 8. a rack; 9. a reset lever; 10. a spring; 11. a baffle; 12. and (5) pressing plates.
Detailed Description
Referring to fig. 1-2, the present utility model provides a technical solution: the utility model provides a serum-free culture medium, including culture dish 1 and sealed lid 2, sealed lid 2 sets up in the upside of culture dish 1, and sealed upper end of lid 2 is provided with go-between 3, and sealed lid 2 cup joints with the go-between 3 screw threads, and sealed lower surface middle part rotation of lid 2 is provided with montant 4, and the pole wall of montant 4 has fixedly cup jointed a plurality of puddlers 5 from last to lower in proper order, and sealed upper surface fixing of lid 2 is provided with fixed shell 6, and the upper end of montant 4 extends to the inside of fixed shell 6, and the inside of fixed shell 6 is provided with drives montant 4 pivoted drive mechanism.
The transmission mechanism comprises a gear 7 and racks 8, the gear 7 is arranged in the fixed shell 6, the gear 7 is fixedly sleeved with the upper end of the vertical rod 4, the two racks 8 are respectively meshed with the two sides of the gear 7, one ends of the two racks 8 are respectively extended to the outer side of the fixed shell 6, reset rods 9 are respectively movably sleeved in the inner ends of the inner sides of the two racks 8, one ends of the reset rods 9 are fixedly connected with the inner wall of the fixed shell 6, springs 10 are movably sleeved on the rod walls of the reset rods 9, and two ends of each spring 10 are respectively fixedly connected with the corresponding racks 8 and the inner wall of the fixed shell 6.
The longitudinal section of the reset rod 9 is square, so that the rack 8 cannot rotate, i.e. can stably slide.
Both sides of the fixed shell 6 are provided with pressing plates 12, and one ends of the outer sides of the two racks 8 are fixedly connected with the corresponding pressing plates 12, so that the racks 8 are conveniently pressed.
The middle ends of the two racks 8 are fixedly sleeved with baffle plates 11, so that the racks 8 are prevented from slipping out of the fixed shell 6 as much as possible.
The outer walls of the two pressing plates 12 are provided with anti-slip rubber mats, friction between the hands and the pressing plates 12 is improved, and slipping during pressing is prevented as much as possible.
When a serum-free culture medium is used, two pressing plates 12 are pressed towards the direction of a fixed shell 6, so that the two pressing plates 12 drive corresponding racks 8 to move, namely, drive gears 7 to rotate, the gears 7 drive vertical rods 4 to rotate, so that a plurality of stirring rods 5 are driven to rotate, then springs 10 push the two racks 8 to reset, during the period, the two racks 8 drive the gears 7 to reversely rotate, namely, drive the vertical rods 4 to reversely rotate, namely, drive a plurality of stirring rods 5 to rotate again, namely, repeatedly pressing the pressing plates 12 on two sides can enable the vertical rods 4 to drive the stirring rods 5 to repeatedly rotate and stir, namely, time-saving and labor-saving stirring can be carried out on the culture medium in the culture dish 1.
Claims (6)
1. Serum-free medium, comprising a culture dish (1) and a sealing cover (2), characterized in that: the utility model discloses a culture dish, including sealed lid (2), fixed shell (6) are provided with sealed lid (2), sealed upper end of lid (2) is provided with go-between (3), sealed lid (2) cup joints with go-between (3) screw thread, sealed lower surface middle part of lid (2) rotates and is provided with montant (4), the pole wall of montant (4) is fixed in proper order from last to having cup jointed a plurality of puddlers (5) down, the upper surface fixing of sealed lid (2) is provided with fixed shell (6), the upper end of montant (4) extends to the inside of fixed shell (6), the inside of fixed shell (6) is provided with the drive montant (4) pivoted drive mechanism.
2. The serum-free medium of claim 1, wherein: the transmission mechanism comprises a gear (7) and racks (8), the gear (7) is arranged in the fixing shell (6), the gear (7) is fixedly sleeved with the upper end of the vertical rod (4), the racks (8) are respectively meshed with the two sides of the gear (7), one ends of the racks (8) are respectively extended to the outer sides of the fixing shell (6), the two ends of the inner ends of the racks (8) are respectively movably sleeved with a reset rod (9), one end of the reset rod (9) is fixedly connected with the inner wall of the fixing shell (6), the rod wall of the reset rod (9) is movably sleeved with a spring (10), and two ends of the spring (10) are respectively fixedly connected with the corresponding racks (8) and the inner wall of the fixing shell (6).
3. A serum-free medium according to claim 2, characterized in that: the longitudinal section of the reset rod (9) is square.
4. A serum-free medium according to claim 2, characterized in that: both sides of the fixed shell (6) are provided with pressing plates (12), and one end of the outer side of each rack (8) is fixedly connected with the corresponding pressing plate (12).
5. A serum-free medium according to claim 2, characterized in that: the middle ends of the two racks (8) are fixedly sleeved with baffle plates (11).
6. The serum-free medium of claim 4, wherein: the outer walls of the two pressing plates (12) are provided with anti-skid rubber mats.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320916795.5U CN219824179U (en) | 2023-04-22 | 2023-04-22 | Serum-free culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320916795.5U CN219824179U (en) | 2023-04-22 | 2023-04-22 | Serum-free culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219824179U true CN219824179U (en) | 2023-10-13 |
Family
ID=88246391
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320916795.5U Active CN219824179U (en) | 2023-04-22 | 2023-04-22 | Serum-free culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219824179U (en) |
-
2023
- 2023-04-22 CN CN202320916795.5U patent/CN219824179U/en active Active
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