CN219792966U

CN219792966U - Mitochondria detection kit

Info

Publication number: CN219792966U
Application number: CN202320432212.1U
Authority: CN
Inventors: 葛新建; 肖青雯
Original assignee: Shanghai Beyotime Biotechnology Co ltd
Current assignee: Shanghai Biyuntian Biotechnology Co ltd
Priority date: 2023-03-09
Filing date: 2023-03-09
Publication date: 2023-10-03
Anticipated expiration: 2033-03-09

Abstract

The utility model discloses a mitochondria detection kit, which belongs to the field of kits, and comprises a reagent bottle main body, wherein the upper end of the reagent bottle main body is in threaded connection with a sealing cover, the top end of the sealing cover is provided with a threaded groove, the threaded groove is internally in threaded connection with a pressurizing cover, the lower end of the sealing cover is internally clamped with a reagent tube, the reagent tube comprises an inner tube body and an outer sleeve, the outer sleeve is sheathed on the outer side of the inner tube body, the inner tube body is clamped with a clamping groove arranged at the bottom end of the sealing cover, the top end of the reagent bottle main body is provided with a conical opening, and the top end of the outer sleeve is fixedly connected with an elastic supporting ring; the inner tube body comprises a rubber chuck matched with the lower end groove of the sealing cover, the lower end of the rubber chuck is fixedly connected with a straight tube part, the lower end of the straight tube part is fixedly connected with a conical containing part matched with the outer sleeve, the liquid in the kit can be treated by using two reagents conveniently, and the quick packaging preservation and reagent treatment after mitochondria extraction are convenient.

Description

Mitochondria detection kit

Technical Field

The utility model relates to the field of kits, in particular to a mitochondrial detection kit.

Background

The kit is a box for containing chemical reagents for detecting chemical components, drug residues, virus types and the like. Is used by general hospitals and pharmaceutical enterprises

The kit for detecting mitochondria needs to use some reagents as fluorescent probes in the kit, and the kit for rapidly and sensitively detecting the change of the cell, tissue or purified mitochondrial membrane potential can be used for early apoptosis detection.

Most of the existing mitochondrial detection kits can only treat one sample or use one reagent for sample, and the detection efficiency is low.

Disclosure of Invention

1. Technical problem to be solved

Aiming at the problems in the prior art, the utility model aims to provide a mitochondrial detection kit which can realize the convenient use of two reagents for treating liquid in the kit and the convenient rapid packaging and preservation after extracting mitochondria and reagent treatment.

2. Technical proposal

In order to solve the problems, the utility model adopts the following technical scheme.

The mitochondrial detection kit comprises a reagent bottle main body, wherein the upper end of the reagent bottle main body is in threaded connection with a sealing cover, the top end of the sealing cover is provided with a threaded groove, a pressurizing cover is in threaded connection with the threaded groove, a reagent tube is clamped in a groove at the lower end of the sealing cover, the reagent tube comprises an inner tube body and an outer sleeve, the outer sleeve is sleeved on the outer side of the inner tube body, the inner tube body is clamped with a clamping groove formed in the bottom end of the sealing cover, the top end of the reagent bottle main body is provided with a conical opening, and the top end of the outer sleeve is fixedly connected with an elastic supporting ring;

the inner pipe body comprises a rubber chuck matched with a lower end groove of the sealing cover, the lower end of the rubber chuck is fixedly connected with a straight pipe portion, the lower end of the straight pipe portion is fixedly connected with a conical accommodating portion matched with the outer sleeve, and a through hole matched with the straight pipe portion is formed in the middle of the sealing cover.

Can realize the convenient use of two reagents to treat the liquid in the kit, and is convenient for rapid packaging and preservation after extracting mitochondria and reagent treatment.

Optionally, a plurality of leak holes have been seted up to the lower extreme of interior body, fixedly connected with a plurality of elasticity bulge loops on the inner wall of outer tube, and the accessible is placed reagent in the outer tube earlier and is handled the interior cell sap of interior body, then contacts interior body and the reagent in the reagent bottle main part, realizes using in proper order of two kinds of reagents.

Optionally, the bottom fixedly connected with of gland is with straight tube portion assorted elasticity sealing block, carries out effective sealing to interior body through the gland.

Optionally, anti-slip patterns are formed on the inner wall in the through hole, so that the inner pipe body is effectively fixed after being inserted into the through hole.

Optionally, a sealing film is laid at the bottom of the thread groove, and is adhered to the bottom of the thread groove, so that the top of the inner pipe body is conveniently sealed by the sealing film after the inner pipe body is inserted into the through hole.

Optionally, the surface of the reagent bottle main body is coated with an anti-illumination coating, so that the influence of external illumination on the reagent in the bottle is reduced.

3. Advantageous effects

Compared with the prior art, the utility model has the advantages that:

(1) The solution can realize that two reagents are conveniently used for treating liquid in the kit, and the fast packaging preservation and reagent treatment after mitochondria extraction are conveniently carried out.

(2) The lower extreme of interior body has offered a plurality of small openings, fixedly connected with a plurality of elasticity bulge loops on the inner wall of outer tube, and the accessible is placed reagent in the outer tube earlier and is handled the cell sap in the interior body, then contacts interior body and the reagent in the reagent bottle main part again, realizes using in proper order of two kinds of reagents.

(3) The bottom fixedly connected with of gland and straight tube portion assorted elasticity sealing block carries out effective seal to the interior body through the gland, has seted up anti-skidding line on the inner wall in the through-hole, guarantees that interior body inserts in the through-hole and is effectively fixed.

(4) The sealing film is laid at the bottom of the thread groove and is adhered to the bottom of the thread groove, and the top of the inner pipe body is conveniently sealed by the sealing film after the inner pipe body is inserted into the through hole.

Drawings

FIG. 1 is a partial perspective view of the present utility model;

FIG. 2 is an exploded view of the present utility model;

FIG. 3 is a cross-sectional view of the present utility model in use;

FIG. 4 is a cross-sectional view of the present utility model after use of the outer sleeve;

fig. 5 is a schematic diagram of the structure at a in fig. 4.

The reference numerals in the figures illustrate:

1 a reagent bottle main body, 2 a sealing cover, 3 a pressurizing cover, 4 an inner tube body, 401 a rubber chuck, 402 a straight tube part, 403 a conical containing part and 5 an outer sleeve.

Detailed Description

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model; it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments, and that all other embodiments obtained by persons of ordinary skill in the art without making creative efforts based on the embodiments in the present utility model are within the protection scope of the present utility model.

In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "inner", "outer", "top/bottom", etc. are directions or positional relationships based on those shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "provided," "engaged," "connected," etc. are to be construed broadly, and may be either fixed, detachable, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

Example 1:

referring to fig. 1-3, a mitochondrial detection kit comprises a reagent bottle main body 1, wherein the surface of the reagent bottle main body 1 is coated with an anti-illumination coating, so that the influence of external illumination on reagents in the bottle is reduced; the upper end of the reagent bottle main body 1 is in threaded connection with a sealing cover 2, the top end of the sealing cover 2 is provided with a thread groove, the thread groove is in threaded connection with a pressurizing cover 3, and a reagent tube is clamped in a groove at the lower end of the sealing cover 2;

the reagent tube comprises an inner tube body 4 and an outer tube 5, the inner tube body 4 and the outer tube 5 are of an integrated structure, the outer tube 5 is sleeved on the outer side of the inner tube body 4, the inner tube body 4 is clamped with a clamping groove formed in the bottom end of a sealing cover 2, a conical opening is formed in the top end of the reagent bottle main body 1, an elastic supporting ring is fixedly connected to the top end of the outer tube 5, a plurality of elastic convex rings are fixedly connected to the inner wall of the outer tube 5, and the elastic convex rings are distributed in the vertical direction at equal intervals;

referring to fig. 3-5, the inner tube 4 includes a rubber chuck 401 matching with a groove at the lower end of the sealing cover 2, a straight tube portion 402 is fixedly connected to the lower end of the rubber chuck 401, a conical accommodating portion 403 matching with the outer sleeve 5 is fixedly connected to the lower end of the straight tube portion 402, and a through hole matching with the straight tube portion 402 is formed in the middle of the sealing cover 2.

The bottom end of the pressurizing cover 3 is fixedly connected with an elastic sealing block matched with the straight pipe part 402, and the inner pipe body 4 is effectively sealed through the pressurizing cover 3. The inner wall in the through hole is provided with anti-skid patterns, so that the inner pipe body 4 is effectively fixed after being inserted into the through hole. The sealing film is laid at the bottom of the thread groove and is adhered to the bottom of the thread groove, and after the inner pipe body 4 is inserted into the through hole, the top of the inner pipe body 4 is conveniently sealed through the sealing film.

When the cell fluid sample is used, the inner tube body 4 or the outer sleeve 5 is used for containing the cell fluid sample, different cell samples can be respectively used in the inner tube body 4 and the outer sleeve 5 and then treated by the same reagent, and when the cell fluid sample is stored in a closed manner, the inner tube body 4 is completely inserted into the through hole of the sealing cover 2, and then the opening of the cell fluid sample is sealed by the pressurizing cover 3;

then the outer sleeve 5 is clamped into the conical opening of the reagent bottle main body 1, finally the sealing cover 2 is clamped and fixed with the reagent bottle main body 1, at the moment, the outer wall of the inner tube 4 is attached to the elastic convex ring in the outer sleeve 5, so that the closed preservation of liquid in the outer sleeve 5 is realized, and at the moment, two kinds of cell liquids treated by the same kind of reagent in the reagent kit are all closed and preserved.

Example 2

For simplicity, only the points of distinction from example 1 are described below. This embodiment 2 is different from embodiment 1 in that:

the lower end of the inner pipe body 4 is provided with a plurality of leakage holes;

the solid cells in the inner tube body 4 can be treated by placing the reagent A in the outer sleeve 5, then the inner tube body 4 is completely inserted into the through hole of the sealing cover 2, the opening of the inner tube body is sealed by the pressurizing cover 3, the sealing cover 2 is fixed with the reagent bottle main body 1, and at the moment, the inner tube body 4 is contacted with the reagent B in the reagent bottle main body 1, so that the two reagents can sequentially treat the fixed cells (the reagent A and the reagent B are selected by a person skilled in the art).

The above description is only of the preferred embodiments of the present utility model; the scope of the utility model is not limited in this respect. Any person skilled in the art, within the technical scope of the present disclosure, may apply to the present utility model, and the technical solution and the improvement thereof are all covered by the protection scope of the present utility model.

Claims

1. The utility model provides a mitochondria detection kit, includes reagent bottle main part (1), its characterized in that: the reagent bottle comprises a reagent bottle main body (1), wherein the upper end of the reagent bottle main body (1) is in threaded connection with a sealing cover (2), the top end of the sealing cover (2) is provided with a threaded groove, the threaded groove is internally in threaded connection with a pressurizing cover (3), a reagent tube is clamped in a groove at the lower end of the sealing cover (2), the reagent tube comprises an inner tube body (4) and an outer sleeve (5), the outer sleeve (5) is sleeved on the outer side of the inner tube body (4), the inner tube body (4) is clamped with a clamping groove formed in the bottom end of the sealing cover (2), the top end of the reagent bottle main body (1) is provided with a conical opening, the top end of the outer sleeve (5) is fixedly connected with an elastic supporting ring, and a plurality of elastic convex rings are fixedly connected on the inner wall of the outer sleeve (5);

the inner pipe body (4) comprises a rubber chuck (401) matched with a lower end groove of the sealing cover (2), a straight pipe portion (402) is fixedly connected to the lower end of the rubber chuck (401), a conical accommodating portion (403) matched with the outer sleeve (5) is fixedly connected to the lower end of the straight pipe portion (402), and a penetrating hole matched with the straight pipe portion (402) is formed in the middle of the sealing cover (2).

2. A mitochondrial assay kit according to claim 1, wherein: the lower end of the inner pipe body (4) is provided with a plurality of leakage holes.

3. A mitochondrial assay kit according to claim 1, wherein: the bottom end of the pressurizing cover (3) is fixedly connected with an elastic sealing block matched with the straight pipe part (402).

4. A mitochondrial assay kit according to claim 1, wherein: and anti-skid patterns are formed on the inner wall in the through hole.

5. A mitochondrial assay kit according to claim 1, wherein: the sealing film is laid at the bottom of the thread groove and is bonded with the bottom of the thread groove.

6. The mitochondrial assay kit of claim 5, wherein: the surface of the reagent bottle main body (1) is coated with an anti-illumination coating.