CN219792934U - Centrifugal tube fixing device of ultrasonic cell disruption instrument - Google Patents
Centrifugal tube fixing device of ultrasonic cell disruption instrument Download PDFInfo
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- CN219792934U CN219792934U CN202320237420.6U CN202320237420U CN219792934U CN 219792934 U CN219792934 U CN 219792934U CN 202320237420 U CN202320237420 U CN 202320237420U CN 219792934 U CN219792934 U CN 219792934U
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- 238000001816 cooling Methods 0.000 claims abstract description 62
- 238000009434 installation Methods 0.000 claims abstract description 12
- 238000005192 partition Methods 0.000 claims description 16
- 230000000149 penetrating effect Effects 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 8
- 238000009210 therapy by ultrasound Methods 0.000 abstract description 5
- 239000002699 waste material Substances 0.000 abstract description 4
- 210000000078 claw Anatomy 0.000 description 11
- 239000005457 ice water Substances 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 239000000498 cooling water Substances 0.000 description 6
- 229920003023 plastic Polymers 0.000 description 5
- 239000004033 plastic Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000002184 metal Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 2
- 238000003466 welding Methods 0.000 description 2
- 241000973497 Siphonognathus argyrophanes Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
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Abstract
The utility model discloses a centrifugal tube fixing device of an ultrasonic cell disruption instrument, which comprises a main body, a cooling mechanism arranged in the main body, a test tube tray arranged in the main body for placing a centrifugal tube and a centrifugal tube fixing mechanism for fixing the centrifugal tube, wherein the test tube tray is detachably arranged in the main body through an installation rod, the cooling mechanism is connected with a test tube on the test tube tray, and the centrifugal tube fixing mechanism is detachably arranged on the test tube tray. The advantage utilizes the test tube tray that can dismantle the setting to fix the test tube, convenient to use stability is good to the cooling body who is connected with the test tube can constantly let in refrigerated circulating water to the test tube in, prevents to influence the stability of preparation sample because of the temperature rise that vibrations produced when ultrasonic treatment, has still promoted work efficiency when cooling is effectual, and can set up a plurality of test tubes in the test tube tray, has reduced the time waste that frequently changes the preparation sample and causes, has further strengthened work efficiency.
Description
Technical Field
The utility model particularly relates to a centrifugal tube fixing device of an ultrasonic cell disruption instrument.
Background
The ultrasonic cell disruption instrument is also called an ultrasonic-microwave synergistic extraction instrument, and the ultrasonic cell disruption instrument is simply an ultrasonic cell disruption instrument which converts electric energy into acoustic energy through a transducer, the acoustic energy is changed into dense small bubbles through a liquid medium, the small bubbles are rapidly broken, and the generated small bubbles are energy like a small bomb, so that the effect of breaking substances such as cells is achieved.
However, the conventional ultrasonic cell disruption apparatus has the following drawbacks:
(1) The ultrasonic crusher is not provided with a cooling device when in use. During the ultrasonic treatment, the temperature of the preparation sample rises due to vibration, and the temperature rises faster under the condition of smaller volume, which affects the stability of the preparation sample. The traditional method is that when the ultrasonic treatment temperature is increased, the test tube is put into an ice water bath beaker for cooling, and the problems of low speed and low efficiency exist.
(2) The ultrasonic crusher is free of test tube fixing devices when in use. During treatment, the ultrasonic crushing head is generally required to be centered, does not adhere to the wall and enters the liquid to be fixed in depth, so that the stability of the test tube is required to be high. The traditional method is that the ultrasonic crushing head is held by hand or placed on a test tube rack, and the hand is easy to shake, so that the ultrasonic crushing head is contacted with the tube wall or the height is changed, and the crushing efficiency is affected.
In chinese patent: 201720435238.6, the name is: the utility model provides a test tube clamping device of ultrasonic cell disruption appearance, is recorded in, including clamping part, the clamping part comprises the three-jaw, including two symmetrical fixed claws and a movable swivel claw, the movable swivel claw passes the claw handle of fixed claw through the screw rod and adopts lock nut locking, screw rod one side is provided with prevents changeing the face, prevent changeing the face and the plane of through-hole one side on the claw handle and be consistent, claw handle tail end is connected to vertical installation pole through telescopic machanism detachably, the movable swivel claw is located two fixed claws vertical neutral planes, the fixed claw all is provided with horizontal arc spacing draw-in groove with the clamping face of movable swivel claw.
Such as the patent: 201720435238.6, the patent mainly uses the clamping part to clamp the test tube, which can achieve the purpose of clamping and fixing the test tube, but has the problems of low stability and no cooling device during use.
Disclosure of Invention
The utility model aims to solve the technical problems that the temperature reduction of the ultrasonic crusher is carried out by using an ice water bath beaker in the using process, the speed is low, the efficiency is low, and the existing ultrasonic crusher has no test tube fixing device or has poor stability in the using process.
Aiming at the technical problems, a centrifugal tube fixing device of an ultrasonic cell disruption instrument is provided; the method is realized by the following technical scheme: the utility model provides an ultrasonic cell breaker centrifuging tube fixing device, includes the main part, sets up cooling body in the main part, sets up the test tube tray of placing the centrifuging tube in the main part and carries out fixed centrifuging tube fixed establishment to the centrifuging tube, the test tube tray can dismantle the setting through the installation pole in the main part, and cooling body and test tube on the test tube tray are connected, and the centrifuging tube fixed establishment can dismantle the setting on the test tube tray.
According to the utility model, the test tube is fixed by using the detachably arranged test tube tray, the use is convenient, the stability is good, and the cooling mechanism connected with the test tube can continuously introduce cooled circulating water into the test tube, so that the stability of a preparation sample is prevented from being influenced by temperature rise generated by vibration during ultrasonic treatment, the working efficiency is improved while the cooling effect is good, a plurality of test tubes can be arranged in the test tube tray, the time waste caused by frequent replacement of the preparation sample is reduced, and the working efficiency is further enhanced.
According to the technical scheme, the partition board is arranged in the main body, the main body is divided into the cooling bin and the motor bin in the vertical direction by the partition board, and different devices arranged in the main body are conveniently separated by the cooling bin and the motor bin, so that the cooling device is convenient to use.
According to the technical scheme of the utility model, the test tube is arranged on the test tube tray, and the test tube tray is detachably arranged in the cooling bin through the mounting rod, so that ice water for cooling can be added into the cooling bin by taking down the test tube tray through the detachable arrangement of the test tube tray, and the use is more convenient.
According to the technical scheme, the cooling mechanism comprises the circulating cooling pipe and the circulating pump, the circulating pump is arranged in the motor bin, one end of the circulating cooling pipe is connected with the circulating pump, the other end of the circulating cooling pipe penetrates through the partition plate and is connected with the test tube, the circulating pump can pump cooling water from the cooling bin to be poured into the test tube, and then the preparation sample is cooled, so that the cooling mechanism is convenient to use, and the cooling speed is high, and meanwhile, the efficiency is improved.
According to the technical scheme, the waterproof arrangement is formed at the penetrating position of the circulating cooling pipe on the partition plate, so that ice water added into the cooling bin cannot leak into the motor bin due to the waterproof arrangement, and the use is safer.
According to the technical scheme, the centrifugal tube fixing mechanism comprises the centrifugal tube supporting plate for supporting the centrifugal tube and the centrifugal tube fixing plate for fixing the centrifugal tube, the centrifugal tube fixing plate is arranged above the centrifugal tube supporting plate, the centrifugal tube can be stably fixed in a test tube through the centrifugal tube supporting plate and the centrifugal tube fixing plate, the centrifugal tube is ensured to be fixedly and stably in ultrasonic vibration, the ultrasonic crushing head is not contacted, and the use stability of the device is ensured.
According to the technical scheme, through holes for penetrating through the centrifuge tube are formed in the centrifuge tube supporting plate and the centrifuge tube fixing plate, the positions of the through holes correspond to each other, and the through holes are formed in the centrifuge tube supporting plate and the centrifuge tube fixing plate, so that the centrifuge tube is convenient to fix and use.
According to the technical scheme, the lower part of the main body is provided with the moving mechanism, the moving mechanism comprises the moving supporting plate and the cross sliding table capable of driving the moving supporting plate to move in the horizontal direction, and the corresponding positions of the centrifugal tube and the ultrasonic crushing head are conveniently adjusted by the moving mechanism, so that the ultrasonic crushing device is convenient to use.
According to the technical scheme, the mounting table main body is detachably arranged on the movable supporting plate through the mounting table, so that the main body is convenient to detach and convenient to use, and daily maintenance is convenient to carry out on the device.
Compared with the prior art, the utility model has the following beneficial effects:
according to the technical scheme, the test tube is fixed by using the detachably arranged test tube tray, the use is convenient, the stability is good, the cooling mechanism connected with the test tube can continuously introduce cooled circulating water into the test tube, the stability of a preparation sample is prevented from being influenced by temperature rise generated by vibration during ultrasonic treatment, the cooling effect is good, meanwhile, the working efficiency is improved, a plurality of test tubes can be arranged in the test tube tray, the time waste caused by frequent replacement of the preparation sample is reduced, and the working efficiency is further enhanced.
Drawings
FIG. 1 is a schematic perspective view of example 1 of the present utility model (with centrifuge tube).
FIG. 2 is a partial cross-sectional view of the present utility model (with centrifuge tubes).
FIG. 3 is a schematic perspective view of the utility model (with centrifuge tubes) except for the main body.
FIG. 4 is an exploded view of the present utility model (with centrifuge tube).
FIG. 5 is a schematic perspective view of example 2 of the present utility model (with centrifuge tubes).
Description of the embodiments
The following describes the technical solution in the embodiment of the present utility model in detail with reference to fig. 1 to 5 in the embodiment of the present utility model.
Example 1
As shown in fig. 1 and 2, an ultrasonic cytoclasis instrument centrifuge tube fixing device comprises a main body 1, a cooling mechanism 2, a test tube tray 3 and a centrifuge tube fixing mechanism 4.
As shown in fig. 2, the main body 1 is a circular metal tube, a partition plate 11 is welded in the main body 1, the partition plate 11 divides the cylindrical tube body of the main body 1 into an upper cavity and a lower cavity, the upper cavity is named as a cooling bin 12, ice water for cooling is placed in the cooling bin 12, the lower cavity is named as a motor bin 13, a motor and a wire harness for driving are arranged in the motor bin 13, in order to ensure that cooling water in the cooling bin 12 cannot leak from a welding position to the motor bin 13, waterproof treatment and waterproof detection should be carried out after the partition plate 11 is welded, and equipment damage caused by leakage of cooling water in the cooling bin 12 from the welding position to the motor bin 13 is prevented.
As shown in fig. 2, 3 and 4, the test tube tray 3 is a circular thin plate provided with test tubes 32, and the diameter of the test tube tray 3 is slightly smaller than the inner diameter of the main body 1, so that the test tube tray 3 can freely rotate in the main body 1, a plurality of circular through holes perpendicular to the test tube tray 3 are uniformly formed in the surface of the test tube tray 3, the test tubes 32 can be fixed in the circular through holes on the test tube tray 3 through glue or other suitable methods, and the end surfaces of the test tubes 32 are flush with the upper surface of the test tube tray 3.
The test tube 32 is one end open-ended cylindrical plastic tubing, in order to be convenient for cool down to the test tube 32 in, opens the water inlet 33 that runs through test tube 32 unilateral in the top of test tube 32, and the one end and the water inlet 33 of circulative cooling pipe 21 are connected, radially evenly offer a plurality of circular shape delivery port 34 along test tube 32 in the lower extreme of test tube 32, and circulative cooling's cooling water flows out test tube 32 via delivery port 34.
The test tube tray 3 can be detachably arranged in the cooling bin 12 through a mounting rod 31, the mounting rod 31 is a metal rod with a regular hexagon cross section, and in order to facilitate the positioning of the mounting rod 31, a positioning block 35 is arranged at the lower end of the mounting rod 31, so that the mounting rod 31 can be accurately arranged in the mounting table 14 arranged on the partition plate 11.
The mounting table 14 is a cylindrical table with a regular hexagonal cross section, a regular hexagonal groove is recessed perpendicular to the upper surface of the mounting table 14, and a positioning groove with a rectangular cross section is recessed for positioning, so that the mounting rod 31 can be inserted into the mounting table 14 according to positioning.
The lower extreme of mount table 14 links together with the driving motor that sets up in motor storehouse 13 to for being convenient for connect, open the through-hole that is convenient for the connection of motor upper shaft pole on baffle 11, can drive test tube tray 3 along installation pole 31 rotation under the effect of motor, and in order to guarantee that the cooling water in the cooling storehouse 12 can not leak motor storehouse 13 from the junction, do waterproof setting at the junction.
As shown in fig. 2, 3 and 4, the centrifuge tube fixing mechanism 4 includes a centrifuge tube pallet 41 for supporting a centrifuge tube and a centrifuge tube fixing plate 42 for fixing the centrifuge tube, the centrifuge tube pallet 41 is pressed on the upper portion of the test tube tray 3, and the centrifuge tube fixing plate 42 is pressed on the upper portion of the centrifuge tube pallet 41.
The centrifuging tube layer board 41 is the plastics disc of PP plastics, radially evenly seted up a plurality of centrifuging tubes at the upper surface perpendicular to upper surface of centrifuging tube layer board 41 and place hole 43, and centrifuging tube place hole 43 and the test tube mouth position on the test tube tray 3 correspond, and for convenient centrifuging tube place, place hole 43 concentric center position at the upper surface of centrifuging tube layer board 41 and the centrifuging tube and indent out a step downwards, the port of centrifuging tube evagination is placed on sunken step, the upper surface of messenger's centrifuging tube port and the upper surface parallel and level of centrifuging tube layer board 41, in addition in order to be convenient for the placing of centrifuging tube, a recess for placing the connection piece between centrifuging tube and the centrifuging tube sealed lid has all been seted up at every centrifuging tube place hole 43 next door.
The centrifuging tube fixed plate 42 is the plastics disc of PP plastics making, and centrifuging tube fixed plate 42 is placed at the upper surface of centrifuging tube layer board 41, and centrifuging tube fixed plate 42's diameter is the same with centrifuging tube layer board 41 and test tube tray 3, and at centrifuging tube fixed plate 42's upper surface perpendicular to upper surface, along centrifuging tube fixed plate 42 radial evenly offered a plurality of and centrifuging tube place the circular through-hole that hole 43 corresponds to the diameter of circular through-hole is the same with centrifuging tube place hole 43 diameter.
In order to be convenient for the fixed of centrifuging tube layer board 41 and centrifuging tube fixed plate 42, at the middle part of centrifuging tube layer board 41 and centrifuging tube fixed plate 42, the perpendicular to surface is opened there is a mounting hole that is convenient for installation pole 31 to pass, through the nut that sets up on installation pole 31, fixes test tube tray 3, centrifuging tube layer board 41 and centrifuging tube fixed plate 42 three together, and the respective position is test tube tray 3 in the layer, centrifuging tube layer board 41 in the centre, centrifuging tube fixed plate 42 is at the top.
As shown in fig. 2 and 3, the cooling mechanism 2 mainly includes a circulation cooling pipe 21 provided in the cooling compartment 12 and a circulation pump provided in the motor compartment 13.
One end of the circulating cooling pipe 21 is connected with a water inlet 33 on the test tube 32, one end of the circulating cooling pipe 21 is led into the motor bin 13 through the partition plate 11, a plurality of circulating cooling pipes 21 connected with the test tube 32 are summarized on one pipe in the motor bin 13 and are connected with a circulating pump arranged in the motor bin 13, a water pumping pipe 22 connected with the circulating pump is arranged in the cooling bin 12 through the partition plate 11, ice water is pumped from the cooling bin 12, the ice water is continuously poured into the test tube 32 through the circulating cooling pipe 21, the centrifuge tube in the test tube 32 is cooled, the purpose of cooling the preparation in the centrifuge tube is achieved, the cooling water in the cooling bin 12 is guaranteed not to leak from the partition plate 11 to the motor bin 13, and waterproof facilities are arranged at the positions of water pipes penetrating through the partition plate 11.
In addition, in order to improve efficiency and reduce time waste caused by frequent replacement of the preparation samples, a centrifuge tube with the preparation samples is arranged in each test tube 32, the test tube tray 3 is driven to rotate by a motor connected with the mounting rod 31 on the test tube tray 3, the preparation samples in the centrifuge tube are cracked one by one in an ultrasonic manner, and the control of the motor rotation is controlled by a control board arranged in the motor bin 13 and a control screen 15 arranged on the main body 1.
The use process of the embodiment is as follows: when the ultrasonic cell crushing device is used, firstly, the test tube tray 3 is taken down, cooling ice water is added into the cooling bin 12, then the test tube tray 3 is installed, then the nut at the upper end of the installation rod 31 is unscrewed, the centrifuge tube fixing plate 42 is taken down, then the centrifuge tube filled with a preparation sample is placed on the centrifuge tube supporting plate 41 through the centrifuge tube placing hole 43, at the moment, the centrifuge tube is integrally placed in the test tube 32, then the centrifuge tube fixing plate 42 is installed, the rotation of the test tube tray 3 is controlled through the control screen 15, and the rotation parameters are matched with the crusher to crush ultrasonic cells.
Example 2
As shown in fig. 5, after the ultrasonic cytoclasis apparatus centrifuge tube fixing device is maintained or moved, the whole position of the device needs to be calibrated, in order to ensure that the crushing head can be accurately inserted into the centrifuge tube every time the ultrasonic cytoclasis is performed, a moving mechanism 5 is arranged below the ultrasonic cytoclasis apparatus centrifuge tube fixing device on the basis of embodiment 1.
The moving mechanism 5 mainly comprises a moving support plate 51 and a cross sliding table 52 capable of driving the moving support plate 51 to move in the horizontal direction, the moving support plate 51 is a rectangular metal plate, the main body 1 is integrally arranged on the upper surface of the moving support plate 51, an installation seat 53 for fixing the main body 1 on the moving support plate 51 is further arranged on the upper surface of the moving support plate 51, the installation seat 53 is a protruding boss, a threaded hole is formed in the surface of the installation seat 53, and the main body 1 is fixed on the moving support plate 51 through a screw.
The cross sliding table 52 can drive the main body 1 to move back and forth, left and right, and is divided into an electric sliding table and a manual sliding table according to the prior art, and can be directly used according to the situation.
The use process of the embodiment is as follows: after the centrifugal tube fixing device of the ultrasonic cell crusher is maintained or cleaned, the centrifugal tube fixing device is connected with the movable supporting plate 51 on the moving mechanism 5 through screws, then the position of the centrifugal tube fixing device is adjusted through the cross sliding table 52, when ultrasonic cells are crushed each time, the crushing head can be accurately inserted into the centrifugal tube, then the test tube tray 3 is taken down, cooling ice water is added into the cooling bin 12, then the test tube tray 3 is installed, then the nut at the upper end of the installation rod 31 is unscrewed, the centrifugal tube fixing plate 42 is taken down, then the centrifugal tube filled with a preparation sample is placed on the centrifugal tube supporting plate 41 through the centrifugal tube placing hole 43, at the moment, the centrifugal tube is integrally placed in the test tube 32, then the centrifugal tube fixing plate 42 is installed, the rotation of the test tube tray 3 is controlled through the control screen 15, and the rotation parameters are matched with the crusher, so that ultrasonic cell crushing is performed.
The above embodiments are only for illustrating the technical idea of the present utility model, and the protection scope of the present utility model is not limited thereto, and any modification made on the basis of the technical scheme according to the technical idea of the present utility model falls within the protection scope of the present utility model.
Claims (9)
1. An ultrasonic cell disruption appearance centrifuging tube fixing device, its characterized in that: including main part (1), cooling body (2) of setting in main part (1), set up test tube tray (3) of placing the centrifuging tube in main part (1) and carry out fixed centrifuging tube fixed establishment (4) to the centrifuging tube, test tube tray (3) can dismantle setting up in main part (1) through installation pole (31), and test tube (32) on cooling body (2) and the test tube tray (3) are connected, and centrifuging tube fixed establishment (4) can dismantle setting up on test tube tray (3).
2. The ultrasonic cytoclasis instrument centrifuge tube fixing device according to claim 1, wherein: a partition plate (11) is arranged in the main body (1), and the partition plate (11) divides the main body (1) into a cooling bin (12) and a motor bin (13) in the vertical direction.
3. The ultrasonic cytoclasis instrument centrifuge tube fixing device according to claim 2, wherein: the test tube tray (3) is provided with a test tube (32), and the test tube tray (3) is detachably arranged in the cooling bin (12) through the mounting rod (31).
4. The ultrasonic cytoclasis instrument centrifuge tube fixing device according to claim 1, wherein: the cooling mechanism (2) comprises a circulating cooling pipe (21) and a circulating pump, wherein the circulating pump is arranged in the motor bin (13), one end of the circulating cooling pipe (21) is connected with the circulating pump, and the other end of the circulating cooling pipe penetrates through the partition plate (11) and is connected with the test tube (32).
5. The ultrasonic cytoclasis instrument centrifuge tube fixing device according to claim 4, wherein: the penetrating part of the circulating cooling pipe (21) on the partition plate (11) is provided with a waterproof arrangement.
6. The ultrasonic cytoclasis instrument centrifuge tube fixing device according to claim 1, wherein: the centrifuge tube fixing mechanism (4) comprises a centrifuge tube supporting plate (41) for supporting the centrifuge tube and a centrifuge tube fixing plate (42) for fixing the centrifuge tube, and the centrifuge tube fixing plate (42) is arranged above the centrifuge tube supporting plate (41).
7. The ultrasonic cytoclasis instrument centrifuge tube fixing device according to claim 1, wherein: through holes for penetrating through the centrifuge tubes are formed in the centrifuge tube supporting plate (41) and the centrifuge tube fixing plate (42), and the positions of the holes are corresponding to each other.
8. The ultrasonic cytoclasis instrument centrifuge tube fixing device according to claim 1, wherein: a moving mechanism (5) is arranged at the lower part of the main body (1), and the moving mechanism (5) comprises a moving supporting plate (51) and a cross sliding table (52) capable of driving the moving supporting plate (51) to move in the horizontal direction.
9. The ultrasonic cytoclasis instrument centrifuge tube fixing device of claim 8, wherein: the movable supporting plate (51) is provided with a mounting seat (53), and the main body (1) is detachably arranged on the movable supporting plate (51) through the mounting seat (53).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320237420.6U CN219792934U (en) | 2023-02-17 | 2023-02-17 | Centrifugal tube fixing device of ultrasonic cell disruption instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320237420.6U CN219792934U (en) | 2023-02-17 | 2023-02-17 | Centrifugal tube fixing device of ultrasonic cell disruption instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219792934U true CN219792934U (en) | 2023-10-03 |
Family
ID=88180730
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320237420.6U Active CN219792934U (en) | 2023-02-17 | 2023-02-17 | Centrifugal tube fixing device of ultrasonic cell disruption instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219792934U (en) |
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2023
- 2023-02-17 CN CN202320237420.6U patent/CN219792934U/en active Active
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