CN219792856U

CN219792856U - Protein purification device

Info

Publication number: CN219792856U
Application number: CN202320972624.4U
Authority: CN
Inventors: 张大生
Original assignee: Changzhou Fuluosen Medical Technology Co ltd
Current assignee: Changzhou Fuluosen Medical Technology Co ltd
Priority date: 2023-04-26
Filing date: 2023-04-26
Publication date: 2023-10-03
Anticipated expiration: 2033-04-26

Abstract

The utility model relates to the technical field of experimental equipment, and particularly provides a protein purification device which comprises an outer tube, wherein a connecting ring is fixedly arranged on the lower surface of a circular ring, clamping holes are symmetrically formed in the outer side of the connecting ring, inserting grooves communicated with the clamping holes are symmetrically formed in the lower surface of the connecting ring, a fixing ring is fixedly arranged on the outer side of the outer tube, elastic inserting pieces which are inserted into the inserting grooves are symmetrically and fixedly arranged on the upper surface of the fixing ring, and clamping blocks which are clamped with the clamping holes are fixedly arranged on the opposite sides of the two elastic inserting pieces. According to the protein purification device, the clamping holes are formed in the clamping blocks in a ejecting mode, so that the elastic plug-in components are elastically bent, the elastic plug-in components and the clamping blocks are pulled out from the inside of the plug-in grooves formed in the connecting rings, the auxiliary plug-in frame and the main plug-in frame are convenient to detach, and the problems that various filter materials or filter screens are usually directly placed in the pipe fittings and fixed when the protein purification device in the prior art is used, and therefore the filter materials or the filter screens are inconvenient to detach and troublesome to clean are solved.

Description

Protein purification device

Technical Field

The utility model relates to the technical field of experimental equipment, in particular to a protein purification device.

Background

Protein separation and purification is an important operation technology in biotechnology application, and separation and purification of high-activity and high-purity proteins are always pursued. At present, a plurality of commercialized protein purification columns exist, the commercialized protein purification columns are high in price and high in use requirement, and the service life of the columns is easily reduced due to careless operation. In addition, the protein separation and purification operation has the problems of complicated operation steps, low aging and the like of the prior protein filtration crude extraction and the like.

The present inventors found that the following problems exist in practical use:

when the protein purification device in the prior art is used, various filter materials or filter screens are usually directly arranged in the pipe fitting and fixed, so that the filter materials or the filter screens are inconvenient to detach and troublesome to clean. Accordingly, there is a need to provide a protein purification apparatus that solves the above-mentioned problems.

Disclosure of Invention

The utility model aims to solve the technical problems that when the protein purification device in the prior art is used, various filter materials or filter screens are usually directly arranged in a pipe fitting and fixed, so that the filter materials or the filter screens are inconvenient to detach and troublesome to clean. According to the protein purification device, the clamping holes are formed in the clamping blocks in a ejecting mode, so that the elastic plug-in components are elastically bent, the elastic plug-in components and the clamping blocks are pulled out from the inside of the plug-in grooves formed in the connecting rings, the auxiliary plug-in frame and the main plug-in frame are convenient to detach, and the problems that various filter materials or filter screens are usually directly placed in the pipe fittings and fixed when the protein purification device in the prior art is used, and therefore the filter materials or the filter screens are inconvenient to detach and troublesome to clean are solved.

In order to achieve the above purpose, the technical scheme of the utility model is as follows: the utility model provides a protein purification device, includes the outer tube, the inside grafting of outer tube has the main frame of inserting, the inside grafting of main frame of inserting has vice frame of inserting, vice frame of inserting is including setting up in the ring at top, the lower fixed surface of ring installs the go-between, the draw-in hole has been seted up to the outside symmetry of go-between, just the draw-in groove with draw-in hole intercommunication has been seted up to the lower surface symmetry of go-between, the outside fixed mounting of outer tube has solid fixed ring, gu fixed ring's upper surface symmetry fixed mounting have the elasticity plug connector with the draw-in groove grafting, two the equal fixed mounting in looks back of elasticity plug connector has the fixture block with the draw-in hole block.

Further, fixing blocks are symmetrically and fixedly arranged on the outer side of the circular ring, and fixing holes are formed in the upper surface of each fixing block.

Further, absorbent cotton is arranged in the auxiliary inserting frame, and a first pocket net is fixedly arranged at the bottom of the auxiliary inserting frame.

The filter paper layer is arranged in the main inserting frame, and the second pocket net is fixedly arranged at the bottom of the main inserting frame.

The device is further characterized in that the lower end of the outer tube is fixedly connected with a liquid outlet tube, and a flow speed control valve is arranged on a pipeline of the liquid outlet tube.

Further, the outside symmetry fixed mounting of main frame that inserts has the montant, every the equal fixedly connected with spiro union post of lower extreme of montant, the outside spiro union of spiro union post has the thread bush, two the lower extreme of thread bush rotates jointly and is connected with the dog.

The guide pipe is further arranged in the stop block in an inserted mode, and the guide pipe is of a Y-shaped structure.

Compared with the related art, the protein purification device provided by the utility model has the following beneficial effects:

the utility model provides a protein purification device, which is characterized in that a clamping block is ejected out of a clamping hole, so that an elastic plug-in connector is elastically bent, and the elastic plug-in connector and the clamping block are pulled out from the inside of a plug-in groove formed in a connecting ring, so that an auxiliary plug-in frame and a main plug-in frame are conveniently detached, and the problems that various filter materials or filter screens are usually directly placed in a pipe fitting and fixed when the protein purification device in the prior art is used, and the filter materials or the filter screens are inconvenient to detach and troublesome to clean are solved.

The utility model provides a protein purification device, which is characterized in that a screw sleeve is rotated to separate from the outer side of a screw connection column, so that a stop block and a main insertion frame are separated, the area of the opposite surface of the stop block and the main insertion frame is conveniently cleaned, the cleaning effect is improved, and the sanitation of equipment is facilitated to be maintained.

Drawings

FIG. 1 is a schematic diagram showing a protein purification apparatus according to a preferred embodiment of the present utility model;

FIG. 2 is a schematic view of the three-dimensional structure of the auxiliary plug frame and the main plug frame of the present utility model;

FIG. 3 is a left side view of the present utility model;

FIG. 4 is an enlarged partial view of the A part of the present utility model;

fig. 5 is a schematic diagram of a front view structure of the connection between the main plug frame and the outer tube.

Reference numerals in the drawings: 1. an outer tube; 2. a fixing ring; 3. a connecting ring; 4. an auxiliary inserting frame; 5. a fixing hole; 6. cotton wool; 7. a circular ring; 8. a fixed block; 9. a liquid outlet pipe; 10. a flow rate control valve; 11. a first net; 12. a main plug frame; 13. a second net; 14. a filter paper layer; 15. a plug-in groove; 16. a clamping hole; 17. a clamping block; 18. an elastic plug-in connector; 19. a screw connection column; 20. a stop block; 21. a flow guiding pipe; 22. a vertical rod; 23. and (5) a threaded sleeve.

Detailed Description

In order that the utility model may be readily understood, a more complete description of the utility model will be rendered by reference to the appended drawings. Exemplary embodiments of the present utility model are illustrated in the accompanying drawings.

Embodiment one:

as shown in fig. 1-5, the protein purification device of the utility model comprises an outer tube 1, a main insertion frame 12 is inserted in the outer tube 1, an auxiliary insertion frame 4 is inserted in the main insertion frame 12, the auxiliary insertion frame 4 comprises a circular ring 7 arranged at the top, a connecting ring 3 is fixedly arranged on the lower surface of the circular ring 7, clamping holes 16 are symmetrically arranged on the outer side of the connecting ring 3, inserting grooves 15 communicated with the clamping holes 16 are symmetrically arranged on the lower surface of the connecting ring 3, a fixing ring 2 is fixedly arranged on the outer side of the outer tube 1, elastic inserting pieces 18 inserted in the inserting grooves 15 are symmetrically and fixedly arranged on the upper surface of the fixing ring 2, clamping blocks 17 clamped with the clamping holes 16 are fixedly arranged on the opposite back surfaces of the two elastic inserting pieces 18, fixing blocks 8 are symmetrically and fixedly arranged on the outer side of the circular ring 7, fixing holes 5 are respectively arranged on the upper surface of each fixing block 8, absorbent cotton 6 is arranged in the auxiliary insertion frame 4, and a first pocket net 11 is fixedly arranged at the bottom of the auxiliary insertion frame 4.

In practice, the clamping block 17 is ejected out of the clamping hole 16, so that the elastic plug-in connector 18 is elastically bent, the elastic plug-in connector 18 and the clamping block 17 are pulled out from the inside of the plug-in groove 15 formed by the connecting ring 3, the auxiliary plug-in frame 4 and the main plug-in frame 12 are convenient to detach, and the problems that various filter materials or filter screens are usually directly placed in the pipe fitting and fixed when the protein purification device in the prior art is used are solved, and the filter materials or the filter screens are inconvenient to detach and troublesome to clean.

Embodiment two:

as shown in fig. 1-5, on the basis of the first embodiment, the present utility model provides a technical solution: the inside of main frame 12 that inserts is provided with filter paper layer 14, and the bottom fixed mounting of main frame 12 has second pocket net 13, the lower extreme fixedly connected with drain pipe 9 of outer tube 1, be provided with flow rate control valve 10 on the pipeline of drain pipe 9, the outside symmetry fixed mounting of main frame 12 has montant 22, the equal fixedly connected with spiro union post 19 of the lower extreme of every montant 22, the outside spiro union of spiro union post 19 has screw sleeve 23, the lower extreme of two screw sleeves 23 rotates jointly and is connected with dog 20, peg graft in dog 20's inside has honeycomb duct 21, honeycomb duct 21 is "Y" type structure.

In practice, the stop block 20 is separated from the main insertion frame 12 by rotating the threaded sleeve 23 to separate from the outer side of the threaded post 19, so that the area of the opposite surface of the stop block 20 and the main insertion frame 12 is conveniently cleaned, the cleaning effect is improved, and the sanitary of equipment is facilitated to be maintained.

When the purification device is used, purification media are firstly added into the outer tube 1, then the main inserting frame 12 is inserted into the outer tube 1, the filter paper layer 14 is placed in the main inserting frame 12, the auxiliary inserting frame 4 is inserted into the main inserting frame 12, the absorbent cotton 6 is placed in the auxiliary inserting frame 4, at the moment, the elastic inserting piece 18 is inserted into the inserting groove 15, at the moment, under the elastic action of the elastic inserting piece 18, the clamping block 17 is clamped into the clamping hole 16, so that the auxiliary inserting frame 4 is fixedly connected with the outer tube 1, finally, an ascites sample is poured into the outer tube 1 from the auxiliary inserting frame 4, macroscopic grease in the ascites is removed from the absorbent cotton 6 above the first pocket net 11 on the auxiliary inserting frame 4, coarse treatment is carried out on the ascites can be carried out on the ascites after the absorbent cotton 6, fine treatment and filtration can be carried out on the filter paper layer 14, the ascites sample after the secondary filtration can fall onto the stop 20, and the ascites sample on the stop 20 flows into the inner part of the outer tube 1 through the guide tube 21 and is discharged through the drain tube 9. When the auxiliary inserting frame 4 and the main inserting frame 12 need to be taken out, the clamping block 17 is firstly ejected out of the clamping hole 16 to enable the elastic inserting piece 18 to elastically bend, then the connecting ring 3 is separated from the fixed ring 2, the elastic inserting piece 18 and the clamping block 17 are separated from the inside of the inserting groove 15 formed by the connecting ring 3, then the auxiliary inserting frame 4 and the main inserting frame 12 are taken out from the inside of the outer tube 1, and finally the threaded sleeve 23 is rotated to enable the threaded sleeve 23 to be separated from the outer side of the threaded post 19, so that the stop block 20 is separated from the main inserting frame 12, and the cleaning is convenient.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. Protein purification device, including outer tube (1), its characterized in that: the inside grafting of outer tube (1) has main frame (12) of inserting, the inside grafting of main frame (12) has vice frame (4) of inserting, vice frame (4) including setting up in ring (7) at top, the lower fixed surface of ring (7) installs go-between (3), draw-in groove (16) have been seted up to the outside symmetry of go-between (3), just draw-in groove (15) with draw-in groove (16) intercommunication have been seted up to the lower surface symmetry of go-between (3), the outside fixed mounting of outer tube (1) has solid fixed ring (2), the upper surface symmetry fixed mounting of solid fixed ring (2) have elastic plug connector (18) with draw-in groove (15) grafting, two the equal fixed mounting in opposite sides of elastic plug connector (18) have fixture block (17) with draw-in groove (16) block.

2. The protein purification device according to claim 1, wherein fixing blocks (8) are symmetrically and fixedly arranged on the outer side of the circular ring (7), and fixing holes (5) are formed in the upper surface of each fixing block (8).

3. The protein purification device according to claim 1, wherein absorbent cotton (6) is arranged in the auxiliary inserting frame (4), and a first net (11) is fixedly arranged at the bottom of the auxiliary inserting frame (4).

4. The protein purification device according to claim 1, wherein a filter paper layer (14) is arranged inside the main insertion frame (12), and a second net (13) is fixedly arranged at the bottom of the main insertion frame (12).

5. The protein purification device according to claim 1, wherein the lower end of the outer tube (1) is fixedly connected with a liquid outlet tube (9), and a flow rate control valve (10) is arranged on the pipeline of the liquid outlet tube (9).

6. The protein purification device according to claim 1, wherein vertical rods (22) are symmetrically and fixedly installed on the outer side of the main plug frame (12), a screw-connection column (19) is fixedly connected to the lower end of each vertical rod (22), a thread sleeve (23) is screwed on the outer side of each screw-connection column (19), and a stop block (20) is connected to the lower ends of the two thread sleeves (23) in a co-rotation mode.

7. The protein purification device according to claim 6, wherein a guide pipe (21) is inserted into the stopper (20), and the guide pipe (21) has a Y-shaped structure.