CN219772105U - Stem cell separation and purification device - Google Patents
Stem cell separation and purification device Download PDFInfo
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- CN219772105U CN219772105U CN202320999525.5U CN202320999525U CN219772105U CN 219772105 U CN219772105 U CN 219772105U CN 202320999525 U CN202320999525 U CN 202320999525U CN 219772105 U CN219772105 U CN 219772105U
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- separation
- treatment box
- stem cell
- purification
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- 238000000926 separation method Methods 0.000 title claims abstract description 46
- 238000000746 purification Methods 0.000 title claims abstract description 42
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 42
- 230000007246 mechanism Effects 0.000 claims abstract description 20
- 210000004027 cell Anatomy 0.000 claims abstract description 14
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 238000007599 discharging Methods 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 238000004113 cell culture Methods 0.000 abstract description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000007790 scraping Methods 0.000 description 3
- 238000003915 air pollution Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
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- Centrifugal Separators (AREA)
Abstract
The utility model relates to the technical field of stem cell culture solution, in particular to a stem cell separation and purification device, which comprises: a base; the separation and purification mechanism is arranged on the base and is used for separating and purifying cells; the filter mechanism is arranged on the separation and purification mechanism and is used for further filtering, the filter mechanism is convenient to install and detach through the cooperation of shielding the cover, rotating the annular groove, positioning rings and the like, the influence of the outside on the separation and purification of stem cells is avoided, people are convenient to use, the vacuum pump is convenient to pump away part of air, the probability of pollution is reduced, the motor, the square block, the square groove and the like are convenient to cooperate, the filter tank is rotated, the stem cells are convenient to centrifugally separate, and the stem cells are convenient to further filter and purify through the cooperation of the motor, the first gear, the first driving wheel, the second gear, the scraper mesh and the like, so that the purity of purification is improved.
Description
Technical Field
The utility model relates to the technical field of stem cell culture solution, in particular to a stem cell separation and purification device.
Background
Stem cells are a class of cells with unlimited or immortalized self-renewal capacity, capable of producing at least one type of highly differentiated daughter cells, and most biologists and medical practitioners consider stem cells to be a class of cells from embryonic, fetal or adult bodies with unlimited self-renewal and proliferative differentiation capacity under certain conditions, capable of producing daughter cells of the exact same phenotype as genotype and self, and capable of producing specialized cells that make up tissues, organs of the body, while also differentiating into progenitor cells.
The stem cell culture solution is subjected to separation and purification by using separation and purification equipment, and the existing separation and purification equipment is easy to be interfered by the outside when the stem cells are separated and purified, so that the stem cell pollution is caused, the purity of the separation and purification is influenced, and the separation and extraction efficiency of the device is reduced.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the utility model provides a stem cell separation and purification device, which solves the problems that in the prior art, separation and purification equipment is easy to be interfered by the outside when separating and purifying stem cells, so that the pollution of the stem cells is caused, the purity of the separation and purification is influenced, and the separation and extraction efficiency of the device is reduced.
(II) technical scheme
In order to achieve the above purpose, the present utility model provides the following technical solutions: a stem cell separation and purification device, comprising:
a base;
the separation and purification mechanism is arranged on the base and is used for separating and purifying cells;
and the filtering mechanism is arranged on the separation and purification mechanism and is used for further filtering.
Preferably, the separation and purification mechanism comprises a plurality of support columns, a motor and a collection box, wherein the support columns and the collection box are fixedly connected to the base, the motor is installed on the base, the support columns are fixedly connected with the treatment box, a shielding cover is installed on the treatment box, the treatment box is rotationally connected with a rotating ring groove, a filter tank is detachably connected to the rotating ring groove, a filter layer is arranged in the filter tank, the bottom end of the filter tank is fixedly connected with a positioning groove and a square groove, the output end of the motor penetrates into the treatment box and is fixedly connected with a square block, the square block and the square groove are matched with each other, a positioning ring is fixedly connected to the inside of the treatment box, the positioning ring and the positioning groove are matched with each other, a plurality of through holes are formed in the positioning ring, the treatment box and the collection box are communicated through a discharge pipe, a valve is installed on the discharge pipe and the discharge pipe, a vacuum pump is communicated to the treatment box, and is used for separating and purifying stem cells, the probability of the stem cells is reduced, and the probability of the occurrence of the dry cells is improved, and the purity of the dry cells is avoided.
Further, the filter mechanism comprises a first driving wheel, a shielding net and two fixing shafts, wherein the first driving wheel is fixedly sleeved on the output end of the motor, the shielding net is installed in the discharging pipe, the two fixing shafts are all rotationally connected between the base and the treatment box, one fixing shaft is fixedly sleeved with a first gear and a second driving wheel, the first driving wheel and the second driving wheel are in transmission connection through a driving belt, the other fixing shaft is fixedly sleeved with a second gear, the first gear is meshed with the second gear, one fixing shaft penetrates into the treatment box and is fixedly connected with the scraping net, and the filter mechanism is used for further filtering dry cells and is convenient for better removing impurities.
Still further, the bottom fixedly connected with of base is a plurality of auto-lock gyro wheels.
Further, a sealing ring is arranged between the cover plate and the processing box.
On the basis of the scheme, the inner bottom wall of the treatment box is an inclined plane.
(III) beneficial effects
Compared with the prior art, the utility model provides a stem cell separation and purification device, which has the following beneficial effects:
1. this stem cell separation and purification device, through shielding the cooperation of lid, rotating annular, holding ring etc, be convenient for install and dismantle the filter vat, avoided the external world to cause the influence to stem cell separation and purification simultaneously, made things convenient for people to use, be convenient for the vacuum pump to take away partial air, reduced the probability that takes place the pollution, be convenient for motor, square groove etc. cooperate, rotate the filter vat, be convenient for carry out centrifugal separation to the stem cell, simultaneously through motor, gear one, drive wheel one, gear two, cooperation such as scraper mesh, be convenient for further filter purification to the stem cell, the purity of purification has been improved.
2. According to the stem cell separation and purification device, the separated cells are placed temporarily through the cooperation of the collecting box, the valve, the discharging pipe and the like, the stem cell separation operation is carried out again through the treatment box, the filtering tank and the like, and the separation and extraction efficiency of the whole device is improved.
Drawings
FIG. 1 is a schematic view of a partially sectioned front plan view effect of the present utility model;
FIG. 2 is a schematic view of a partial enlarged structure of the present utility model at A in FIG. 1;
FIG. 3 is a schematic view of a partially enlarged structure of the present utility model at B in FIG. 1;
fig. 4 is a schematic view of a partially enlarged structure of fig. 1C according to the present utility model.
In the figure: 1. a base; 2. a support column; 3. a motor; 4. a collection box; 5. a treatment box; 6. a shielding cover; 7. rotating the ring groove; 8. a filter tank; 9. a filter layer; 10. a positioning groove; 11. a square groove; 12. a square block; 13. a positioning ring; 14. a through hole; 15. a discharge pipe; 16. a valve; 17. a vacuum pump; 18. a shielding net; 19. a fixed shaft; 20. a first gear; 21. a transmission belt; 22. a second gear; 23. a scraper screen; 24. self-locking idler wheels; 25. and (3) a sealing ring.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Examples
Referring to fig. 1-4, a stem cell separation and purification device includes:
a base 1;
in the figures 1-3, the separation and purification mechanism is arranged on the base 1, the separation and purification mechanism comprises a plurality of support columns 2, a motor 3 and a collection box 4, the support columns 2 and the collection box 4 are fixedly connected on the base 1, the motor 3 is arranged on the base 1, the plurality of support columns 2 are fixedly connected with a treatment box 5, a shielding cover 6 is arranged on the treatment box 5, a rotary ring groove 7 is rotationally connected in the treatment box 5, a filter tank 8 is detachably connected on the rotary ring groove 7, a filter layer 9 is arranged in the filter tank 8, the bottom end of the filter tank 8 is fixedly connected with a positioning groove 10 and a square groove 11, the output end of the motor 3 penetrates into the treatment box 5 and is fixedly connected with a square block 12, the square block 12 is matched with the square groove 11, a positioning ring 13 is fixedly connected in the treatment box 5, the positioning ring 13 is matched with the positioning groove 10, a plurality of through holes 14 are formed in the positioning ring 13, the treatment box 5 and the collection box 4 are communicated through the discharge pipe 15, the collection box 4 is communicated with the discharge pipe, valves 16 are arranged on the discharge pipe and the discharge pipe 15, the treatment box 5 is communicated with the vacuum pump 17, the motor 3, the square block 12, the positioning ring 13, the positioning groove 10 and the like are used for driving the filter tank 8 and the filter layer 9 to rotate and are used for filtering cell suspension, the separation and purification of stem cells are facilitated, the vacuum pump 17 is used for pumping out part of air, the probability of air pollution to the cells is reduced, the treatment box 5, the shielding cover 6, the filter tank 8 and the like are used for avoiding external interference, and the purity of the separation and purification of the stem cells is improved;
in fig. 1 and 4, the filtering mechanism is arranged on the separation and purification mechanism, the filtering mechanism comprises a first driving wheel, a shielding net 18 and two fixing shafts 19, the first driving wheel is fixedly sleeved on the output end of the motor 3, the shielding net 18 is arranged in the discharging pipe 15, the two fixing shafts 19 are both rotatably connected between the base 1 and the processing box 5, a first gear 20 and a second driving wheel are fixedly sleeved on one of the fixing shafts 19, the first driving wheel and the driving wheel are in transmission connection through a driving belt 21, a second gear 22 is fixedly sleeved on the other fixing shaft 19, the first gear 20 is meshed with the second gear 22, one fixing shaft 19 penetrates through the processing box 5 and is fixedly connected with a scraping plate net 23, the shielding net 18 is used for further filtering stem cells, the first gear 20, the second gear 22, the first driving wheel, the scraping plate net 23 and the like are used for cleaning the shielding net 18, and blocking of the shielding net 18 is prevented.
In fig. 1, a plurality of self-locking rollers 24 are fixedly connected to the bottom end of the base 1, so as to facilitate movement of people.
In fig. 1 and 2, a sealing ring 25 is provided between the cover plate and the processing box 5, and functions as a seal.
In fig. 1, the inner bottom wall of the treatment tank 5 is beveled to facilitate better drainage of the isolated stem cells.
It should be further described that, the motor 3 in this embodiment is a conventional device known to those skilled in the art and purchased in the market, and can be selected or customized according to actual needs, in this patent, only the motor 3 is used, its structure and function are not improved, its setting mode, installation mode and electrical connection mode are not improved, for those skilled in the art, only the debugging operation is required according to the requirement of the instruction, and the motor 3 is not repeated herein, and the mounting position of the control switch is selected according to the actual use requirement, so that the operator can operate and control.
In summary, the working principle and the working process of this stem cell separation and purification device are that, when using, firstly place this stem cell separation and purification device in the place that needs to use, then open shielding lid 6 earlier, put into filter tank 8 with cell suspension, then close shielding lid 6, start vacuum pump 17 and extract some air, be used for reducing the probability of taking place the air pollution, then start motor 3, motor 3 can drive square 12, drive wheel etc. and rotate, be convenient for square 12, square groove 11, constant head tank 10 etc. cooperate, rotate filter tank 8, be convenient for filter tank 8 and filter layer 9 filter the cell suspension, be convenient for carry out centrifugal separation, then the stem cell that has separated can fall into the bottom of handling case 5, drive wheel one is convenient for drive wheel two through drive belt 21 and rotate, be convenient for drive wheel two drive gear one 20 rotate, be convenient for gear one drive gear two 22 rotate, because gear one 20 pinion takes gear two 22 big gears, fixed axle 19 on gear two 22 can drive scraper mesh 23 slowly rotate, thereby be convenient for clear up and shelter mesh 18, the filter mesh 18 has been avoided carrying out the separation to shelter from the filter mesh to the cell, the filter mesh that is convenient for shelter from the secondary to carry out on the filter mesh 18, thereby the purity has been improved when shelter mesh 18 has been carried out.
The foregoing examples merely illustrate specific embodiments of the utility model, which are described in greater detail and are not to be construed as limiting the scope of the utility model. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the utility model, which are all within the scope of the utility model.
Claims (6)
1. A stem cell separation and purification device, comprising:
a base;
the separation and purification mechanism is arranged on the base and is used for separating and purifying cells;
and the filtering mechanism is arranged on the separation and purification mechanism and is used for further filtering.
2. The stem cell separation and purification device according to claim 1, wherein: the separation and purification mechanism comprises a plurality of support columns, a motor and a collection box, wherein the support columns and the collection box are fixedly connected to a base, the motor is installed on the base, the support columns are fixedly connected with a treatment box, a shielding cover is installed on the treatment box, a rotating ring groove is connected in the treatment box in a rotating mode, a filter tank is detachably connected to the rotating ring groove, a filter layer is arranged in the filter tank, the bottom end of the filter tank is fixedly connected with a positioning groove and a square groove, the output end of the motor penetrates into the treatment box and is fixedly connected with a square block, the square block is matched with the square groove, a positioning ring is fixedly connected to the inside of the treatment box, the positioning ring is matched with the positioning groove, a plurality of through holes are formed in the positioning ring, the treatment box is communicated with the collection box through a discharge pipe, valves are arranged on the discharge pipe and the discharge pipe, and a vacuum pump is communicated with the treatment box.
3. The stem cell separation and purification device according to claim 2, wherein: the filtering mechanism comprises a first driving wheel, a shielding net and two fixing shafts, wherein the first driving wheel is fixedly sleeved on the output end of the motor, the shielding net is installed in the discharging pipe, the two fixing shafts are all rotatably connected between the base and the treatment box, one of the fixing shafts is fixedly sleeved with a first gear and a second driving wheel, the first driving wheel and the second driving wheel are in driving connection through a driving belt, the other fixing shaft is fixedly sleeved with a second gear, the first gear is meshed with the second gear, and one fixing shaft penetrates through the treatment box and is fixedly connected with a scraper net.
4. A stem cell separation and purification apparatus according to claim 3, wherein: the bottom fixedly connected with of base is a plurality of auto-lock gyro wheels.
5. The stem cell separation and purification device according to claim 4, wherein: a sealing ring is arranged between the shielding cover and the processing box.
6. The stem cell separation and purification device according to claim 5, wherein: the inner bottom wall of the treatment box is an inclined plane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320999525.5U CN219772105U (en) | 2023-04-27 | 2023-04-27 | Stem cell separation and purification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320999525.5U CN219772105U (en) | 2023-04-27 | 2023-04-27 | Stem cell separation and purification device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219772105U true CN219772105U (en) | 2023-09-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320999525.5U Active CN219772105U (en) | 2023-04-27 | 2023-04-27 | Stem cell separation and purification device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219772105U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117586859A (en) * | 2023-11-28 | 2024-02-23 | 上海多宁生物科技股份有限公司 | Stem cell conditioned medium purification device and application method thereof |
-
2023
- 2023-04-27 CN CN202320999525.5U patent/CN219772105U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117586859A (en) * | 2023-11-28 | 2024-02-23 | 上海多宁生物科技股份有限公司 | Stem cell conditioned medium purification device and application method thereof |
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