CN219730923U - Combined magnetic bead reaction tube convenient to control - Google Patents
Combined magnetic bead reaction tube convenient to control Download PDFInfo
- Publication number
- CN219730923U CN219730923U CN202320633063.5U CN202320633063U CN219730923U CN 219730923 U CN219730923 U CN 219730923U CN 202320633063 U CN202320633063 U CN 202320633063U CN 219730923 U CN219730923 U CN 219730923U
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- plate body
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- magnetic
- magnetic bead
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- 239000011324 bead Substances 0.000 title claims abstract description 70
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 59
- 230000007704 transition Effects 0.000 claims description 9
- 229910000828 alnico Inorganic materials 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 229910052761 rare earth metal Inorganic materials 0.000 claims description 3
- 150000002910 rare earth metals Chemical class 0.000 claims description 3
- 229910000859 α-Fe Inorganic materials 0.000 claims description 3
- 238000010354 CRISPR gene editing Methods 0.000 description 7
- 108091033409 CRISPR Proteins 0.000 description 4
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 4
- 101710116602 DNA-Binding protein G5P Proteins 0.000 description 4
- 101710162453 Replication factor A Proteins 0.000 description 4
- 101710176758 Replication protein A 70 kDa DNA-binding subunit Proteins 0.000 description 4
- 101710176276 SSB protein Proteins 0.000 description 4
- 101710126859 Single-stranded DNA-binding protein Proteins 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000004417 polycarbonate Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000018120 Recombinases Human genes 0.000 description 2
- 108010091086 Recombinases Proteins 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000009144 enzymatic modification Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010841 mRNA extraction Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a combined magnetic bead reaction tube convenient to control, wherein a first plate body is sleeved above the outer wall of a lower tube body and is flush with an opening of the lower tube body, the first plate body is arranged in an extending way along the horizontal direction towards one side of the lower tube body, the outer side of an extending section of the first plate body is arranged in an opening and closing way with one end of a second plate body, the upper end of the outermost side of the extending section of the first plate body is contacted with the lower side of one end of the second plate body, a lower tube cover is arranged at the position corresponding to the opening of the lower tube body, and the outer diameter of the lower tube body is equal to the inner diameter of the opening of the lower tube body; the upper pipe body corresponding to the lower pipe cover is fixed on the second plate body, the lower end of the third plate body is fixedly connected with the other end of the second plate body, the upper end of the third plate body is flush with the upper pipe body, one end of the fourth plate body is opened and closed with the upper end of the third plate body, a magnetic body which can be inserted into the upper pipe body is fixed on one side of the other end of the fourth plate body, and the height of the magnetic body is the same as that of the upper pipe body.
Description
Technical Field
The utility model relates to the field of biomedical treatment, in particular to a combined magnetic bead reaction tube convenient to control.
Background
Magnetic beads have been widely used in immunodetection, cell separation, biological macromolecule purification, and molecular biology since the 90 th century as a novel extraction reagent that is frequently used in nucleic acid extraction, immunology, molecular purification, and the like.
The magnetic beads have special physicochemical properties, for example, the reaction rate can be reduced in a reaction system, and the reaction result is affected. The addition of magnetic beads in the experimental process leads to the need of uncovering in the reaction process, which not only can pollute the sample, but also can cause aerosol pollution to influence the reaction. For samples with biological hazard, the released aerosol can cause great harm to the health and safety of operators.
Disclosure of Invention
Aiming at the problems in the prior art, the utility model provides a combined magnetic bead reaction tube which is convenient to control, can release magnetic beads under the condition of not uncovering, and realizes the function of adsorbing the magnetic beads.
The utility model is realized by the following technical scheme:
a combined magnetic bead reaction tube convenient to control comprises a lower tube body, an upper tube body, a first connecting piece and a second connecting piece;
the lower pipe body is in an opening arrangement, the first connecting piece comprises a first plate body and a second plate body, the first plate body is sleeved above the outer wall of the lower pipe body, the openings of the first plate body and the lower pipe body are flush, the first plate body extends downwards to one side of the lower pipe body along the horizontal direction, the second plate body is of a planar structure, the outer side of the extending section of the first plate body and one end of the second plate body are arranged in an opening and closing manner, the upper end of the outermost side of the extending section of the first plate body is contacted with the lower side of one end of the second plate body, a cylindrical lower pipe cover is arranged at the position, corresponding to the opening of the lower pipe body, of the second plate body, the outer diameter of the lower pipe cover is equal to the inner diameter of the opening of the lower pipe body, and the other end of the second plate body is positioned at one side of the lower pipe body;
the second plate body on be fixed with the corresponding last body of lower tube cap position, the second connecting piece includes third plate body and fourth plate body, the lower extreme of third plate body and the other end fixed connection of second plate body, the upper end of going up the body and the upper end and the last body parallel and level of third plate body are kept away from to the upper end of third plate body, the one end of fourth plate body and the upper end setting that opens and shuts of third plate body, one side that the fourth plate body other end down is fixed with the magnetic body that can insert the last body, the magnetic body is the same with the height of last body.
Preferably, the magnetic plate further comprises a cylindrical upper tube cover fixed at the other end of the fourth plate body towards the lower side, the height of the upper tube cover is equal to or smaller than that of the upper tube body, the magnetic body is in the shape of a cylinder with the outer diameter equal to the inner diameter of the upper tube body, and the magnetic body is fixedly inserted into the upper tube body.
Further, the height of the upper pipe cover is smaller than that of the upper pipe body, and the lower end of the magnetic body is located below the upper pipe cover.
Preferably, the upper pipe cover, the lower pipe body, the upper pipe body, the first connecting piece and the second connecting piece are all made of PC plastic.
Preferably, the magnetic material is a ferrite magnet, a rare earth magnet, an alnico magnet, or a polymer magnetic compound.
Preferably, the height of the upper pipe body is 3-8mm.
Preferably, the number of the upper pipe bodies and the number of the magnetic bodies are the same and are a plurality of, and the plurality of upper pipe bodies are uniformly arranged on the second plate body.
Preferably, the first plate body and the second plate body are of an integrated structure, and the third plate body and the fourth plate body are of an integrated structure.
Preferably, the lower end of the third plate body is perpendicular to the other end of the second plate body.
Preferably, the lower pipe body comprises a cylindrical section, a transition section and a circular table section which are integrally structured from top to bottom, the outer diameter of the upper end of the transition section is the same as the outer diameter of the lower end of the cylindrical section, the outer diameter of the lower end of the transition section is the same as the outer diameter of the upper end of the circular table section, the lower end of the lower pipe body is in a semicircle shape or a cone shape, and the diameter of the bottom surface of the semicircle or cone is equal to the diameter of the bottom surface of the circular table section;
the inner diameter of the cylindrical section is the same as the outer diameter of the lower pipe cover.
Compared with the prior art, the utility model has the following beneficial technical effects:
the cylindrical lower tube cover can be tightly inserted into the opening of the lower tube body, the first plate body and the second plate body can be combined together to be contacted with each other, the tightness of the magnetic bead reaction is ensured, and the other end of the second plate body is positioned at one side of the lower tube body, so that the lower tube cover can be conveniently opened after the reaction is finished. The fourth plate body drives the magnetic body to contact with the second plate body after being inserted into the upper pipe body, magnetic beads required by the reaction can be adsorbed in the lower pipe cover through magnetic force, then the magnetic body is opened according to the requirement, the magnetic beads can participate in the reaction, the magnetic body is inserted into the upper pipe body after the reaction is finished, the combined magnetic bead reaction pipe is inverted, and the reaction can be completed through adsorbing all the magnetic beads, so that the functions of placing the magnetic beads and recovering the magnetic beads are achieved. The utility model utilizes the magnetic body to control the release of the magnetic beads, and adopts the lower tube cover to store the magnetic beads, thereby avoiding the defect that the traditional reaction device needs to cover the cover halfway to add the magnetic beads in the reaction process, and effectively solving the problems of pollution and biological hazard caused by cover opening in the reaction process.
Drawings
FIG. 1 is a schematic view of a magnetic bead reaction tube according to the present utility model.
FIG. 2 is a schematic view of another view angle structure of the magnetic bead reaction tube according to the present utility model.
In the figure: 1-magnetic body, 2-upper tube cover, 3-lower tube cover, 4-lower tube body, 5-first connecting piece, 6-upper tube body, 7-second connecting piece.
Detailed Description
The utility model is further illustrated by the following figures and examples, which are not intended to be limiting.
The utility model relates to a combined magnetic bead reaction tube convenient to control, which comprises a magnetic body 1, an upper tube cover 2, a lower tube cover 3, a lower tube body 4, a first connecting piece 5, an upper tube body 6 and a second connecting piece 7, wherein the first connecting piece 5 comprises a first plate body and a second plate body, and the second connecting piece 7 comprises a third plate body and a fourth plate body.
Specifically, as shown in fig. 1 and fig. 2, on the one hand, the lower tube body 4 is used for bearing a reaction system, a common structure is adopted for design, the upper end of the lower tube body 4 is provided with an opening, the main structure of the first plate body is a circular ring, the circular ring is sleeved above the outer wall of the lower tube body 4 and is flush with the opening of the lower tube body 4, the circular ring extends to one side of the lower tube body 4 along the horizontal direction, the extension section is selected to be rectangular, the second plate body is of a plate-shaped structure with a planar structure, the outer side of the extension section of the first plate body is arranged to be opened and closed with one end of the second plate body, meanwhile, the outermost upper end of the extension section is contacted with the lower side of one end of the second plate body, so that the second plate body can be contacted with the first plate body, the position of the second plate body corresponding to the opening of the lower tube body 4 is provided with a lower tube cover 3, the outer diameter is equal to the inner diameter of the opening of the lower tube body 4, so that the lower tube cover 3 can be tightly inserted into the opening of the lower tube body 4, the other end of the second plate body is arranged to be positioned on one side of the lower tube body 4, and the second plate body is conveniently opened by the triangle.
On the other hand, the magnetic beads can be various according to the reaction requirement of the magnetic bead method, therefore, a plurality of upper tube bodies 6 corresponding to the positions of the lower tube cover 3 are fixed on the second plate body, the magnetic beads with different specifications are fixed on the lower tube cover 3, the magnetic beads with different specifications are stored on the second plate body, the lower end of the third plate body is perpendicular to the upper surface of the second plate body, the lower end of the third plate body is fixedly connected with the other end of the second plate body, the third plate body is far away from the upper tube body 6 and is flush with the upper tube body 6, one end of the fourth plate body is opened and closed with the upper end of the third plate body, the magnetic bodies 1 which can be inserted into the upper tube body 6 are fixed on one side of the other end of the fourth plate body, the upper tube body 6 and the magnetic bodies 1 are identical in number and are a plurality, the plurality of upper tube bodies 6 are uniformly arranged on the second plate body at intervals, meanwhile, the height of the magnetic bodies 1 and the upper tube bodies 6 are required to be ensured to be identical, the magnetic bodies 1 can be contacted with the second plate body after being inserted into the upper tube bodies 6, and the magnetic bodies can be adsorbed on the lower tube covers 3 through magnetic force. If more upper pipe bodies 6 are required to be designed, the upper pipe bodies 6 can be designed on the second plate body in 2-3 rows according to the structure, and the specific number can be designed according to the actual size.
In order to ensure that the magnetic body 1 is more firmly installed, the utility model also designs an upper tube cover 2 fixed on the downward side of the other end of the fourth plate body, the upper tube cover 2 is also in a cylinder shape, the height of the upper tube cover is equal to or smaller than that of the upper tube body 6, the shape of the magnetic body 1 is required to be a cylinder with the outer diameter equal to the inner diameter of the upper tube body 6, the magnetic body 1 is fixedly inserted into the upper tube body 6, and the magnetic beads are released by opening the upper tube cover 2 in a set reaction time. In order to save materials and labor, the height of the upper tube cover 2 is designed to be smaller than that of the upper tube body 6, so that the lower end of the magnetic body 1 leaks out and is positioned below the upper tube cover 2.
In order to facilitate the manufacture of the combined magnetic bead reaction tube, the materials of the upper tube cover 2, the lower tube cover 3, the lower tube body 4, the first connecting piece 5, the upper tube body 6 and the second connecting piece 7 are PC plastics, namely polycarbonate. The first plate body and the second plate body are opened and closed originally, and the third plate body and the fourth plate body are opened and closed in a plurality of modes, such as most common hinging, because the materials are selected, the first plate body and the second plate body are manufactured into an integral structure, and the third plate body and the fourth plate body are manufactured into an integral structure more conveniently, so that the integral structure is selected.
The height of the upper pipe body 6 can be 3-8mm, the specific shape of the lower pipe body 4 comprises a cylindrical section, a transition section and a circular table section which are integrally formed from top to bottom, so that the outer diameter of the upper end of the transition section is the same as that of the lower end of the cylindrical section, the outer diameter of the lower end of the transition section is the same as that of the upper end of the circular table section, and the lower end of the lower pipe body 4 is in a semicircle shape or a cone shape, and thus the diameter of the bottom surface of the semicircle or cone is required to be equal to the diameter of the bottom surface of the circular table section, and the inner diameter of the cylindrical section is the same as that of the lower pipe cover 3.
The magnetic body 1 may be used including, but not limited to, ferrite magnets, rare earth magnets, alnico magnets, and polymer magnetic compounds.
The utility model relates to a combined magnetic bead reaction tube convenient to control, which is characterized in that when in use, the following conditions are described:
when nucleic acid extraction reaction is carried out, silicon hydroxyl magnetic beads are adopted; when mrna extraction and purification are performed, oligonucleotide (dT) -coated magnetic beads are required for the surface; when performing RPA-CRISPR reactions, magnetic beads with specific enzymatic modifications on the surface are required, including first, RPA reaction system magnetic beads: magnetic beads of surface-modified recombinase, magnetic beads of surface-modified single-stranded DNA binding protein (SSB), magnetic beads of surface-modified strand-displacement DNA polymerase; second, CRISPR reaction system magnetic beads: the magnetic beads with the surface modified CRISPR-CAS12 and the magnetic beads with the surface modified probes are required to be added in a set time and sequence, the magnetic beads are released according to the sequence required by the reaction, the magnetic beads are adsorbed to the original positions for storage in the time without the participation of the magnetic beads, and the influence on the reaction or detection of the system caused by the dissipation of the magnetic beads in the system is avoided, so that the reaction efficiency is improved. The utility model takes RPA-CRISPR reaction as an example to design a combined magnetic bead reaction tube, and for other reactions which are not mentioned or not mentioned, the upper tube covers 2 with corresponding numbers can be designed according to the same thought.
The utility model relates to a conveniently controlled combined magnetic bead reaction tube, taking RPA-CRISPR reaction as an example, and the operation process is as follows:
before the reaction starts, a sample of the target detection strain was added to the lower tube 4, and 25. Mu.L of each of the upstream and downstream primers (10. Mu.M) of the reaction enhancer (10X) was added thereto, and the temperature of the lower tube 4 was heated to 90℃for 5 minutes to facilitate the cell lysis of the target detection strain. At this time, starting the reaction, when the temperature of the lower tube body 4 is reduced to 30 ℃, adding magnetic beads of an RPA reaction system, including magnetic beads of surface modified recombinase, magnetic beads of surface modified single-stranded DNA binding protein (SSB) and magnetic beads of surface modified strand displacement DNA polymerase, and opening one of the upper tube covers 2 above the magnetic beads at this time to finish the addition;
after the RPA reaction is completed, another CRISPR reaction system is added, wherein the CRISPR reaction system comprises magnetic beads with surface modified CRISPR-CAS12 and magnetic beads with probes on the surfaces, at the moment, the addition can be completed by opening the other upper tube cover 2 above the magnetic beads, then the upper tube cover 2 is inserted into the upper tube body 6, when the CRISPR is completed, the magnetic beads are required to be adsorbed, the combined magnetic bead reaction tube is only required to be inverted, and the magnetic body 1 can adsorb all the magnetic beads to complete the reaction, so that the functions of respectively placing different magnetic beads and recovering the magnetic beads are achieved.
Claims (10)
1. The combined magnetic bead reaction tube is characterized by comprising a lower tube body (4), an upper tube body (6), a first connecting piece (5) and a second connecting piece (7);
the lower pipe body (4) is in an opening arrangement, the first connecting piece (5) comprises a first plate body and a second plate body, the first plate body is sleeved above the outer wall of the lower pipe body (4), the first plate body is flush with the opening of the lower pipe body (4), the first plate body extends downwards to one side of the lower pipe body (4) along the horizontal direction, the second plate body is in a plane structure, the outer side of the extending section of the first plate body and one end of the second plate body are arranged in an opening-closing manner, the outermost upper end of the extending section of the first plate body is contacted with the lower side of one end of the second plate body, a cylindrical lower pipe cover (3) is arranged at the position corresponding to the opening of the lower pipe body (4), the outer diameter of the lower pipe cover (3) is equal to the inner diameter of the opening of the lower pipe body (4), and the other end of the second plate body is positioned on one side of the lower pipe body (4).
The second plate body on be fixed with down corresponding last body (6) of tube cap (3) position, second connecting piece (7) are including third plate body and fourth plate body, the lower extreme of third plate body and the other end fixed connection of second plate body, the upper end of going up body (6) and the upper end and last body (6) parallel and level of third plate body are kept away from to the upper end of third plate body, the one end of fourth plate body and the upper end setting that opens and shuts of third plate body, one side that the fourth plate body other end down is fixed with magnetic body (1) that can insert last body (6), magnetic body (1) are the same with the height of last body (6).
2. The combined magnetic bead reaction tube convenient to control according to claim 1, further comprising a cylindrical upper tube cover (2) fixed on the other end of the fourth plate body at the downward side, wherein the height of the upper tube cover (2) is equal to or smaller than that of the upper tube body (6), the magnetic body (1) is in the shape of a cylinder with the outer diameter equal to the inner diameter of the upper tube body (6), and the magnetic body (1) is fixedly inserted into the upper tube body (6).
3. The combined magnetic bead reaction tube convenient to control according to claim 2, wherein the height of the upper tube cover (2) is smaller than that of the upper tube body (6), and the lower end of the magnetic body (1) is positioned below the upper tube cover (2).
4. The magnetic bead reaction tube of claim 2, wherein the upper tube cover (2), the lower tube cover (3), the lower tube body (4), the upper tube body (6), the first connecting piece (5) and the second connecting piece (7) are all made of PC plastic.
5. The combined magnetic bead reaction tube convenient to control according to claim 1, wherein the magnetic body (1) is a ferrite magnet, a rare earth magnet, an alnico magnet or a high molecular magnetic compound.
6. A modular magnetic bead reaction tube according to claim 1, characterized in that the height of the upper tube body (6) is 3-8mm.
7. The combined magnetic bead reaction tube convenient to control according to claim 1, wherein the number of the upper tube bodies (6) and the number of the magnetic bodies (1) are the same, and the plurality of the upper tube bodies (6) are uniformly arranged on the second plate body.
8. The magnetic bead reaction tube of claim 1, wherein the first plate and the second plate are integrally formed, and the third plate and the fourth plate are integrally formed.
9. The conveniently controlled combined magnetic bead reaction tube according to claim 1, wherein the lower end of the third plate is perpendicular to the other end of the second plate.
10. The combined magnetic bead reaction tube convenient to control according to claim 1, wherein the lower tube body (4) comprises a cylindrical section, a transition section and a circular table section which are integrally structured from top to bottom, the outer diameter of the upper end of the transition section is the same as the outer diameter of the lower end of the cylindrical section, the outer diameter of the lower end of the transition section is the same as the outer diameter of the upper end of the circular table section, the lower end of the lower tube body (4) is a semicircle or a cone, and the diameter of the bottom surface of the semicircle or the cone is equal to the diameter of the bottom surface of the circular table section;
the inner diameter of the cylindrical section is the same as the outer diameter of the lower pipe cover (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320633063.5U CN219730923U (en) | 2023-03-27 | 2023-03-27 | Combined magnetic bead reaction tube convenient to control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320633063.5U CN219730923U (en) | 2023-03-27 | 2023-03-27 | Combined magnetic bead reaction tube convenient to control |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219730923U true CN219730923U (en) | 2023-09-22 |
Family
ID=88030802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320633063.5U Active CN219730923U (en) | 2023-03-27 | 2023-03-27 | Combined magnetic bead reaction tube convenient to control |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219730923U (en) |
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2023
- 2023-03-27 CN CN202320633063.5U patent/CN219730923U/en active Active
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