CN219695141U - Novel fluorescence immunochromatography kit - Google Patents
Novel fluorescence immunochromatography kit Download PDFInfo
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- CN219695141U CN219695141U CN202320082749.XU CN202320082749U CN219695141U CN 219695141 U CN219695141 U CN 219695141U CN 202320082749 U CN202320082749 U CN 202320082749U CN 219695141 U CN219695141 U CN 219695141U
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- strip
- box body
- detection
- shaped box
- line
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- 238000003317 immunochromatography Methods 0.000 title claims abstract description 12
- 238000001514 detection method Methods 0.000 claims abstract description 62
- 238000007789 sealing Methods 0.000 claims abstract description 22
- 238000010521 absorption reaction Methods 0.000 claims abstract description 6
- 230000000712 assembly Effects 0.000 claims abstract description 4
- 238000000429 assembly Methods 0.000 claims abstract description 4
- 239000011248 coating agent Substances 0.000 claims description 15
- 238000000576 coating method Methods 0.000 claims description 15
- 238000003908 quality control method Methods 0.000 claims description 11
- 230000000007 visual effect Effects 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000004587 chromatography analysis Methods 0.000 abstract description 5
- 238000001125 extrusion Methods 0.000 abstract description 3
- 238000006073 displacement reaction Methods 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 4
- 239000000427 antigen Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model relates to the technical field of clinical medicine detection, in particular to a novel fluorescent immunochromatography kit, which comprises a strip-shaped box body with a hollow structure and a detection strip arranged in the strip-shaped box body, wherein two ends of the strip-shaped box body are provided with openings, two ends of the strip-shaped box body are provided with sealing assemblies, and a limiting assembly is arranged between the strip-shaped box body and the detection strip. Compared with the prior art, the novel fluorescent immunochromatography kit provided by the utility model can not only avoid the problem of unsmooth chromatography caused by large-area extrusion or pollution formed between the detection strip and the top wall of the strip-shaped box body, but also avoid the problem of left-right displacement of the detection strip in the strip-shaped box body through the limiting component; the sealing assembly can avoid false positive or moisture absorption caused by the influence of external environment on the detection structure.
Description
Technical Field
The utility model relates to the technical field of clinical medicine detection, in particular to a novel fluorescent immunochromatography kit.
Background
In the fluorescent immunochromatography method, the double-antibody sandwich method is used for detection in a solid-phase immunochromatography mode. The serum/plasma/whole blood of the sample to be detected is upwards diffused by capillary force at the sample adding end, and PCT antigen in the sample and the marker are combined into a compound of labeled antibody-antigen when passing through the conjugate pad; the complex diffuses onto a nitrocellulose membrane along with a sample, is intercepted by a detection line of a region coated with PCT antibody to form an immune complex of a labeled antibody-antigen-coated antibody, and reacts when the label continuously moves forwards along the membrane to a quality control line, the fluorescence color development intensity at the detection line is in direct proportion to the PCT content, and the PCT concentration is quantitatively determined according to the fluorescence intensity. Patent CN210742273U discloses a kit for fluorescent chromatography by double antibody sandwich method, which detects related indexes based on dry immunofluorescence method through structures such as sample pad, binding pad and the like and a structure of an outer box sleeve with an opening, but the structure of a cutting sleeve is not provided with height limiting teeth, when detection is carried out, the height limiting teeth are lacking to cause that a detection strip is extruded or polluted to influence the chromatography effect, and further cause the problem of influence of false positive and the like; meanwhile, a sealing plug is lack at the opening end, so that the problems of false positive and the like affecting the accuracy are easily caused by moisture absorption or infection during detection.
Disclosure of Invention
Aiming at overcoming the defects of the background technology, the utility model provides a novel fluorescent immunochromatography kit.
The technical scheme adopted by the utility model is as follows: a novel fluorescence immunochromatography kit is characterized in that: the detection device comprises a strip-shaped box body with a hollow structure and a detection strip arranged in the strip-shaped box body, wherein two ends of the strip-shaped box body are provided with openings, two ends of the strip-shaped box body are provided with sealing assemblies, and a limiting assembly is arranged between the strip-shaped box body and the detection strip; the limiting assembly comprises two groups of height limiting frames, the two groups of height limiting frames are respectively close to two ends of the strip-shaped box body, each group of height limiting frames comprises two L-shaped rods arranged on two sides of the detection strip, the vertical parts of the L-shaped rods are fixedly connected with the bottom wall of the strip-shaped box body, and the transverse parts of the L-shaped rods are right opposite to each other and are in contact with the top wall of the strip-shaped box body.
Preferably, the detection strip comprises a strip-shaped bottom plate and a coating film laid on the strip-shaped bottom plate, wherein a detection line and a C line quality control line are arranged on the coating film, a sample pad and a bonding pad are arranged at one end, close to the detection line, of the coating film, and a water absorption pad is arranged at one end, close to the C line quality control line, of the coating film.
Preferably, the detection line comprises a T1 detection line, a T2 detection line and a T3 detection line which are sequentially arranged, and the T3 detection line is close to a C line quality control line.
Preferably, a visual window and a sample adding hole are formed in the top of the strip-shaped box body, the visual window is located above the coating film, and the sample adding hole is located above the sample pad.
Preferably, the sealing assembly comprises a sealing plug matched with the end face of the strip-shaped box body, and a step part is arranged on the inner surface of the sealing plug and is tightly attached to the inner wall of the strip-shaped box body.
The beneficial effects are that: compared with the prior art, the novel fluorescent immunochromatography kit provided by the utility model can not only avoid the problem of unsmooth chromatography caused by large-area extrusion or pollution formed between the detection strip and the top wall of the strip-shaped box body, but also avoid the problem of left-right displacement of the detection strip in the strip-shaped box body through the limiting component; the sealing assembly can avoid false positive or moisture absorption caused by the influence of external environment on the detection structure.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a top view of FIG. 1;
FIG. 3 is a cross-sectional view A-A of FIG. 1;
fig. 4 is a schematic structural view of the closure assembly.
Detailed Description
The present utility model will be described in detail below with reference to the drawings and detailed description to enable those skilled in the art to better understand the technical scheme of the present utility model.
As shown in fig. 1-4, a novel fluorescence immunochromatography kit comprises a strip-shaped box body 1 with a hollow structure and a detection strip 2 arranged in the strip-shaped box body 1, wherein two ends of the strip-shaped box body 1 are provided with openings, two ends of the strip-shaped box body 1 are provided with sealing assemblies 3, and a limiting assembly 4 is arranged between the strip-shaped box body 1 and the detection strip 2; the limiting assembly 4 comprises two groups of height limiting frames 4, the two groups of height limiting frames 4 are respectively close to two ends of the strip-shaped box body 1, each group of height limiting frames 4 comprises two L-shaped rods 41 arranged on two sides of the detection strip 2, vertical parts of the L-shaped rods 41 are fixedly connected with the bottom wall of the strip-shaped box body 1, and transverse parts of the L-shaped rods 41 are right opposite to each other and are in contact with the top wall of the strip-shaped box body 1. During production, the detection strip is inserted from the openings at the two ends of the strip-shaped box body, the detection strip is completely arranged between the vertical parts of the two L-shaped rods 41, so that the detection strip can be effectively prevented from shifting left and right in the strip-shaped box body, and the problem of unsmooth chromatography caused by extrusion or pollution due to the fact that the transverse part of the L-shaped rod 41 is in large-area contact with the top wall of the strip-shaped box body due to overlarge thickness of the detection strip can be avoided, and the openings at the two ends of the strip-shaped box body are sealed through the sealing assembly, so that the strip-shaped box body is in a sealing structure, the detection strip can be protected, and other reagents or environmental pollution can be effectively avoided; the kit provided by the utility model can be disposable, or can be used for multiple times after the detection strip is taken out and sterilized according to requirements and a new detection strip is replaced.
In this embodiment, the test strip 2 includes a strip-shaped bottom plate 21 and a coating film 22 laid on the strip-shaped bottom plate 21, a detection line and a C-line quality control line 23 are provided on the coating film 22, a sample pad 24 and a bonding pad 25 are provided at one end of the coating film 22 near the detection line, and a water absorption pad 26 is provided at one end of the coating film 22 near the C-line quality control line 23. Specifically, the coating film is a nitrocellulose film, the material of the bonding pad is glass fiber, and the bonding pad is sprayed with high-concentration coupling antibody, and in the preferred scheme, the coupling antibody is one of colloidal gold, colored latex, colloidal selenium or fluorescence.
In this embodiment, the detection lines include a T1 detection line 27, a T2 detection line 28, and a T3 detection line 29 that are sequentially set, where the T3 detection line 29 is disposed near the C line quality control line 23. Specifically, the user accurately judges through a plurality of detection lines and a quality control line C line.
In this embodiment, a visual window 5 and a sample adding hole 6 are provided at the top of the strip-shaped box body 1, the visual window 5 is located above the coating film 22, and the sample adding hole 6 is located above the sample pad 24. Specifically, the user can titrate reagent at the application of sample hole to see through visual window direct observation detection line T1, T2, T3 and matter control line C line, improve the accuracy that detects by a wide margin.
In this embodiment, the sealing assembly 3 includes a sealing plug 31 that is matched with the end surface of the strip-shaped box body 1, and a step portion 32 is disposed on the inner surface of the sealing plug 31, and the step portion 32 is tightly attached to the inner wall of the strip-shaped box body. When the detection device is used, the sealing plug covers the end face of the strip-shaped box body, the step part enters the strip-shaped box body and is tightly attached to the inner wall of the strip-shaped box body, so that a closed space is formed in the inner cavity of the strip-shaped box body, the problem that the accuracy is affected due to the fact that the detection strip is excessively exposed and is easy to be infected when detection is carried out is solved.
In a preferred scheme, the sealing plug is made of rubber. The structure can enable the sealing plug to be in closer fit with the strip-shaped box body, so that a better sealing effect is achieved, and external dampness and infection are avoided.
Finally, it should be noted that the above description is only a preferred embodiment of the present utility model, and that many similar changes can be made by those skilled in the art without departing from the spirit and scope of the utility model as defined in the appended claims.
Claims (5)
1. A novel fluorescence immunochromatography kit is characterized in that: the detection device comprises a strip-shaped box body (1) with a hollow structure and a detection strip (2) arranged in the strip-shaped box body (1), wherein two ends of the strip-shaped box body (1) are provided with openings, two ends of the strip-shaped box body (1) are provided with sealing assemblies (3), and a limiting assembly (4) is arranged between the strip-shaped box body (1) and the detection strip (2);
limiting component (4) are including two sets of limit for height frame (41), and two sets of limit for height frame (41) are close to respectively the both ends setting of bar box body (1), every group limit for height frame (41) including set up in two L type poles of detection strip (2) both sides, the vertical portion of L type pole with the diapire fixed connection of bar box body (1), the horizontal portion of L type pole just sets up, and with the roof contact of bar box body (1).
2. The novel fluorescent immunochromatographic kit according to claim 1, wherein: the detection strip (2) comprises a strip-shaped bottom plate (21) and a coating film (22) laid on the strip-shaped bottom plate (21), wherein a detection line and a C line quality control line (23) are arranged on the coating film (22), a sample pad (24) and a bonding pad (25) are arranged at one end, close to the detection line, of the coating film (22), and a water absorption pad (26) is arranged at one end, close to the C line quality control line (23).
3. The novel fluorescent immunochromatographic kit according to claim 2, wherein: the detection line comprises a T1 detection line (27), a T2 detection line (28) and a T3 detection line (29) which are sequentially arranged, and the T3 detection line (29) is close to the C line quality control line (23).
4. The novel fluorescent immunochromatographic kit according to claim 2, wherein: the top of the strip-shaped box body (1) is provided with a visual window (5) and a sample adding hole (6), the visual window (5) is positioned above the coating film (22), and the sample adding hole (6) is positioned above the sample pad (24).
5. The novel fluorescent immunochromatographic kit according to claim 1, wherein: the sealing assembly (3) comprises a sealing plug (31) matched with the end face of the strip-shaped box body (1), a step part (32) is arranged on the inner surface of the sealing plug (31), and the step part (32) is tightly attached to the inner wall of the strip-shaped box body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320082749.XU CN219695141U (en) | 2023-01-28 | 2023-01-28 | Novel fluorescence immunochromatography kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320082749.XU CN219695141U (en) | 2023-01-28 | 2023-01-28 | Novel fluorescence immunochromatography kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219695141U true CN219695141U (en) | 2023-09-15 |
Family
ID=87964307
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320082749.XU Active CN219695141U (en) | 2023-01-28 | 2023-01-28 | Novel fluorescence immunochromatography kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219695141U (en) |
-
2023
- 2023-01-28 CN CN202320082749.XU patent/CN219695141U/en active Active
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