CN219687912U

CN219687912U - Liquid storage device

Info

Publication number: CN219687912U
Application number: CN202320619220.7U
Authority: CN
Inventors: 王玄; 王春峰
Original assignee: Shanghai Ruiyu Biotech Co Ltd
Current assignee: Shanghai Ruiyu Biotech Co Ltd
Priority date: 2023-03-24
Filing date: 2023-03-24
Publication date: 2023-09-15
Anticipated expiration: 2033-03-24

Abstract

Embodiments of the present disclosure provide a liquid storage device, including: a shell with an inner cavity, wherein a groove is formed in the inner side surface of the bottom wall of the shell; the first connector is arranged on the shell and is provided with a first channel communicated with the inner cavity; the inner tube is arranged in the inner cavity, one end of the inner tube is communicated with the first channel in the first joint, and the other end of the inner tube extends into the groove.

Description

Liquid storage device

Technical Field

The present disclosure relates to the field of cell culture devices, and more particularly to a liquid storage device.

Background

Cell culture can be used to culture a few cells in large quantities into simple single cells or few differentiated multicells, which is an essential process for the whole bioengineering technique. In the current cell culture field, in cell culture, it is necessary to continuously feed a cell culture solution to a culture chamber through a liquid storage bottle or a liquid storage bag while receiving and discharging metabolites in cell culture. The screw cap of the common liquid storage bottle is provided with a joint for connecting the pipelines, and the processing and assembling processes are complicated. Meanwhile, when the liquid storage bottle absorbs liquid in the bottle through the internal connection hose, the contact position between the hose and the bottle bottom is not well controlled, and liquid residues exist in the bottle at the filling end point, so that the loss of the culture solution is caused. The liquid storage bag is a soft bag formed by welding films, the production process has high technical threshold and is not easy to realize, and the liquid storage bag needs to be hung above the culture cavity to realize the perfusion effect, so that the occupied space is large.

Therefore, it is necessary to provide a liquid storage device. The liquid storage device has small production and processing difficulty, can effectively reduce the residual quantity of liquid and flexibly utilizes the space.

Disclosure of Invention

One of the embodiments of the present disclosure provides a liquid storage device, including: a shell with an inner cavity, wherein a groove is formed in the inner side surface of the bottom wall of the shell; the first connector is arranged on the shell and is provided with a first channel communicated with the inner cavity; the inner tube is arranged in the inner cavity, one end of the inner tube is communicated with the first channel in the first joint, and the other end of the inner tube extends into the groove.

In some embodiments, the groove comprises a stepped recess structure, the bottom surface of the groove is recessed to form a sub-groove, and the other end of the inner tube extends into the sub-groove.

In some embodiments, the reservoir device further comprises: the second connector is arranged on the shell and is provided with a second channel communicated with the inner cavity.

In some embodiments, the housing further comprises a side wall and a top wall, the bottom wall and the top wall are respectively located at a lower end and an upper end of the side wall along a vertical direction of the housing, and the second connector and the first connector are both disposed on the top wall.

In some embodiments, the inner cavity is provided with a baffle, the bottom edge of the baffle is connected to the bottom wall, and the baffle is positioned between projection positions of the second joint and the first joint on the bottom wall along the vertical direction of the shell respectively; a gap is formed between a portion of at least one side edge of the partition plate, which is close to the bottom edge, and the side wall of the shell.

In some embodiments, the top wall includes a first region and a second region, wherein the first region is a greater distance from the bottom wall than the second region is from the bottom wall.

In some embodiments, the second joint and the first joint are both disposed on the second region.

In some embodiments, the second joint and the first joint are detachably sealed joints.

In some embodiments, the second fitting comprises a luer fitting, and the reservoir device further comprises a luer plug mated with the luer fitting, with a waterproof, breathable membrane disposed on the luer plug.

In some embodiments, the number of the shells is a plurality, each shell is correspondingly provided with the first joint and the second joint, the shells are sequentially connected, and the second joint on one shell is connected with the first joint on the next shell for any two adjacent shells in the shells.

Drawings

The present specification will be further elucidated by way of example embodiments, which will be described in detail by means of the accompanying drawings. The embodiments are not limiting, in which like numerals represent like structures, wherein:

FIG. 1A is a schematic diagram of a fluid reservoir device according to some embodiments of the present disclosure;

FIG. 1B is a schematic illustration of the internal structure of a fluid reservoir device according to some embodiments of the present disclosure;

FIG. 2A is a schematic illustration of a further fluid reservoir apparatus according to some embodiments of the present disclosure;

FIG. 2B is a schematic illustration of the internal structure of yet another fluid reservoir device according to some embodiments of the present disclosure;

FIG. 3 is a schematic illustration of the internal structure of yet another fluid reservoir apparatus according to some embodiments of the present disclosure;

FIG. 4 is a schematic diagram of a groove according to some embodiments of the present disclosure;

FIG. 5 is a schematic illustration of a further groove according to some embodiments of the present disclosure;

FIG. 6 is a schematic illustration of a further groove according to some embodiments of the present disclosure;

FIG. 7 is a schematic illustration of a further fluid reservoir apparatus according to some embodiments of the present disclosure;

FIG. 8 is a schematic illustration of the internal structure of a reservoir with a baffle according to some embodiments of the present disclosure;

FIG. 9 is a schematic illustration of the internal structure of a further reservoir with a baffle according to some embodiments of the present disclosure;

fig. 10 is a schematic structural view of yet another liquid storage device according to some embodiments of the present disclosure.

Reference numerals illustrate: 100. a liquid storage device; 110. a housing; 120. a first joint; 130. a second joint; 140. an inner tube; 121. a first channel; 131. a second channel; 111. a top wall; 111-1, a first region; 111-2, a second region; 112. a bottom wall; 113. a sidewall; 114. a groove; 114-1, sub-grooves; 150. a partition plate; 151. a top edge; 152. a side edge; 153. a bottom edge; 160. and (5) connecting pipes.

Detailed Description

In order to more clearly illustrate the technical solutions of the embodiments of the present specification, the drawings that are required to be used in the description of the embodiments will be briefly described below. It is apparent that the drawings in the following description are only some examples or embodiments of the present specification, and it is possible for those of ordinary skill in the art to apply the present specification to other similar situations according to the drawings without inventive effort. Unless otherwise apparent from the context of the language or otherwise specified, like reference numerals in the figures refer to like structures or operations.

It will be appreciated that "system," "apparatus," "unit" and/or "module" as used herein is one method for distinguishing between different components, elements, parts, portions or assemblies at different levels. However, if other words can achieve the same purpose, the words can be replaced by other expressions.

As used in this specification and the claims, the terms "a," "an," "the," and/or "the" are not specific to a singular, but may include a plurality, unless the context clearly dictates otherwise. In general, the terms "comprises" and "comprising" merely indicate that the steps and elements are explicitly identified, and they do not constitute an exclusive list, as other steps or elements may be included in a method or apparatus.

The present specification provides a reservoir that may be used for perfusion culture of cells. In cell perfusion culture, it is necessary to convey a cell culture solution into a culture chamber and collect a cell culture waste liquid generated in cell culture.

The reservoir may be used to store a cell culture fluid or other fluid (e.g., a cell culture waste fluid) as desired during cell culture. In the process of cell perfusion culture, the culture solution in the liquid storage device can be conveyed into the culture cavity from the inner cavity in the liquid storage device under the power action of an external pump, so that the culture solution in the cell culture process is continuously updated, and the requirement of cells on nutrition is timely met. Meanwhile, the cell culture waste liquid containing harmful metabolites such as lactic acid, ammonium ions and the like generated in the cell culture process can be removed through the liquid storage device.

Fig. 1A is a schematic structural view of a liquid storage device according to some embodiments of the present disclosure, and fig. 1B is a schematic structural view of an internal structure of a liquid storage device according to some embodiments of the present disclosure. As shown in fig. 1A, 1B, the reservoir 100 may include a housing 110 having an interior cavity, a first connector 120, and an inner tube 140. The inner side of the bottom wall 112 of the housing 110 is provided with a recess 114. The first connector 120 is disposed on the housing 110, and is provided with a first channel 121 that communicates with the inner cavity. The inner tube 140 is disposed in the inner cavity, one end of the inner tube 140 is communicated with the first channel 121 in the first joint 120, and the other end of the inner tube 140 extends into the groove.

The liquid storage device 100 is used for storing liquid. The reservoir 100 includes a housing 110 having an interior cavity. The housing 110 may be various forms. For example, the housing 110 may be a cuboid, cube, cylinder, cone, or other shape. The interior cavity of the housing 110 may be used to contain a liquid. The interior cavity within the housing 110 may be a variety of shapes formed by a plurality of wall-like structures. As shown in fig. 1B, when the housing 110 is placed horizontally, the surface of the housing 110 that contacts the horizontal surface is the bottom wall 112 of the housing 110. In some embodiments, the housing 110 may also include side walls and a top wall. As shown in fig. 1B, the housing 110 may include a top wall 111 and a plurality of side walls 113 in addition to the bottom wall 112. Wherein the bottom wall 112 and the top wall 111 are located at a lower end and an upper end of the side wall 113 in the vertical direction of the housing 110. In some embodiments, the material of the housing 110 may be one or more of polystyrene, glass, polypropylene, and polymethyl methacrylate.

As shown in fig. 1B, the bottom surface of the recess 114 is lower than the inner side surface of the bottom wall 112. The bottom surface of the recess 114 is the lowest surface of the cavity when the reservoir 100 is placed horizontally. As the liquid in the lumen gradually decreases, the liquid will eventually pool in the groove 114. The grooves 114 may be in the form of various recessed structures. As shown in fig. 3, the grooves 114 may be concave structures of spheres. As shown in fig. 4, the groove 114 may also be a concave structure that communicates directly with the side wall 113. As shown in fig. 5, the inner side surface of the bottom wall 112 of the housing 110 and the bottom edge of the groove 114 may also be inclined in the same direction, and the groove 114 communicates with the side wall 113.

The first connector 120 may be disposed at various locations on the housing 110. For example, the first joint 120 may be provided on the top wall 111 or the side wall 113.

As shown in fig. 1B, a first passage 121 communicating with the inner cavity of the housing 110 may be provided in the first joint 120. One end of the first passage 121 communicates with the outside, and the other end communicates with the inner cavity of the housing 110. Liquid may flow into or out of the interior cavity of the housing 110 through the first passage 121 via the first connector 120.

As shown in fig. 1B, the inner tube 140 may be disposed within the interior cavity of the housing 110. One end of the inner tube 140 may be in communication with the first channel 121 in the first joint 120, and the other end of the inner tube 140 may extend into the groove 114. In some embodiments, the inner tube 140 may be a rigid tube. For example, the inner tube 140 may be a glass tube. In some embodiments, the inner tube 140 may also be a flexible tube with the other end secured within the groove 114. For example, the inner tube 140 may also be a polyvinyl chloride tube, one end of which may be secured against movement within the groove 114. The inner tube 140 may be of various tubular structures. For example, the inner tube 140 may be a hollow cylinder structure or other hollow structure with bends.

In some embodiments of the present disclosure, a groove is formed in an inner side surface of the bottom wall, and the liquid in the groove can be obtained through an inner tube extending into the groove, so that a residual amount of the liquid in the inner cavity can be effectively controlled, and waste of the cell culture liquid is reduced.

In some embodiments, the reservoir 100 may further include a second connector 130. As shown in fig. 2A and 2B, the second connector 130 is disposed on the housing 110, and a second channel 131 is disposed in communication with the inner cavity.

Similar to the first connector 120, the second connector 130 may be disposed at various locations on the housing 110. One end of the second passage 131 communicates with the outside, and the other end communicates with the inner cavity of the housing 110. Liquid may flow into or out of the interior cavity of the housing 110 through the second passage 131 via the second connector 130.

In some embodiments, the second connector 130 may be co-walled with the first connector 120. For example, the second connector 130 and the first connector 120 may both be disposed on the top wall 111. In some embodiments of the present disclosure, the second connector 130 and the first connector 120 are disposed on the top wall 111, so that, on one hand, the effective space in the interior can be utilized to the maximum extent when the liquid is input into the inner cavity, and on the other hand, the inner tube 140 can be kept in a emptied state when the liquid storage device 100 is produced and processed, so as to avoid damage caused by touching the bottom wall 112 by the inner tube 140. In other embodiments, the second connector 130 and the first connector 120 may be provided on different walls. For example, the second connector 130 may be disposed on the top wall 111 and the first connector 120 may be disposed on the side wall 113.

As shown in fig. 6, the groove 114 may have a stepped recess structure, and a bottom surface of the groove 114 may have a recess to form the sub-groove 114-1. When the sub-groove 114-1 is present, the bottom surface of the sub-groove 114-1 is the lowest surface of the cavity when the liquid storage device 100 is placed horizontally. As the liquid within the lumen gradually decreases, the liquid will eventually pool within the sub-groove 114-1. When the sub-groove 114-1 is present, the other end of the inner tube 140 may extend into the sub-groove 114-1.

Some embodiments of the present disclosure further design a sub-groove 114-1 in the groove 114, and extend the other end of the inner tube 140 to the sub-groove 114-1, so that the residual amount of the liquid in the inner cavity can be further reduced based on the original groove 114, and the waste of the cell culture liquid can be reduced.

In some embodiments, the second joint 130 and the first joint 120 may be a detachable sealing joint.

In some embodiments, the second connector 130 may be a luer connector. Some embodiments of the present disclosure provide for a luer fitting that can be mated with other components (e.g., consumables with mating luer fitting) by providing the second fitting with a luer fitting, thereby ensuring that it can be looped or otherwise closed with other components for fluid storage, extraction, and recycling, and convenience of use. Meanwhile, the pollution, leakage and the like possibly caused to the liquid when other parts are additionally used for conveying the liquid can be avoided. When the second fitting is a luer fitting, the reservoir may also include a luer plug that mates with the luer fitting. For example, the second fitting 130 may be an external luer fitting and the reservoir device may include an internal luer plug that mates with the external luer fitting. In some embodiments, a waterproof and breathable membrane may be provided on the luer plug. In some embodiments of the present description, external air may be allowed into the lumen by the luer plug providing a waterproof, breathable membrane, so that the pressure in the lumen may be balanced as liquid flows through the lumen to the culture chamber. Meanwhile, the waterproof breathable film can isolate external bacteria, so that bacterial pollution is avoided, and cell culture results are influenced.

In some embodiments, the first connector 120 may be an in-line hose connector. An external hose can be connected with the in-line hose connector to form a channel for the passage of liquid. In other embodiments, the first connector 120 may also be a luer connector.

The bottom wall 112 and the side wall 113 of the housing 110 may be integrally formed by injection molding. The top wall 111 of the housing 110 may also be obtained by injection molding, and the obtained top wall 111 may include predetermined hole sites of the second connector 130 and the first connector 120. The second connector 130 and the first connector 120 may be welded to predetermined holes of the top wall 111. The inner tube 140 is assembled with the first joint 120, and then the second joint 130, the first joint 120, and the top wall 111 and the bottom wall 112 and the side wall 113 of the inner tube 140 are welded, thereby obtaining the liquid storage device 100. When the second connector 130 and the first connector 120 are disposed at other positions of the housing 110, the liquid storage device 100 may also be obtained in a similar manner as described above.

Compared with a liquid storage bag and a liquid storage bottle, some embodiments of the specification can be produced and processed more simply by the mode to obtain the liquid storage device, so that the difficulty of a production process is reduced.

Fig. 7 is a schematic structural view of yet another liquid storage device according to some embodiments of the present disclosure.

In some embodiments, the top wall 111 may be zoned and the distance of the different zones from the bottom wall 112 may be adjusted to fit different spaces.

As shown in fig. 7, the top wall 111 may include a first region 111-1 and a second region 111-2. Wherein the distance between the first region 111-1 and the bottom wall 112 is greater than the distance between the second region 111-2 and the bottom wall 112, and the second joint 130 and the first joint 120 may be disposed on the second region 111-2. In some embodiments, the second connector 130 and the first connector 120 may also be disposed on the first region 111-1.

In some embodiments of the present disclosure, the height of the second connector 130 and the first connector 120 can be reduced by dividing each area of the top wall 111 and adjusting the distance between the second area 111-2 and the bottom wall 112, so that the liquid storage device 100 forms a stepped structure, and the second connector 130 and the first connector 120 are disposed on the second area 111-2, so as to solve the problem of insufficient space.

In some embodiments, a baffle 150 may also be included in the interior cavity of the housing 110. As shown in fig. 8 and 9, the second joint 130 and the first joint 120 may be located at different sides of the partition 150, respectively. The baffle 150 can include a top edge 151, side edges 152, and a bottom edge 153, and the bottom edge 153 of the baffle 150 can be connected to the bottom wall 112 of the interior cavity of the housing 110 and positioned between the projected positions of the second connector 130 and the first connector 120, respectively, on the bottom wall 112 along the vertical direction of the housing 110. At least a portion of one side 152 of the diaphragm 150 adjacent the bottom edge 153 is in clearance with the side wall 113 of the housing 110 for fluid communication. A gap may be provided between the top edge 151 of the baffle 150 and the top wall 111 of the housing 110. Top edge 151 of diaphragm 150 may also be connected to top wall 111 of housing 110 (as shown in fig. 9).

The second connector 130 may be separated from the first connector 120 by a spacer 150 in some embodiments of the present disclosure. When the liquid flows into the inner cavity from one joint, the liquid needs to flow in the inner cavity for a relatively long distance and then flows out from the other joint, so that the phenomenon that the mixture of various inflow liquids is insufficient and the cell culture effect is influenced due to the fact that the liquid flowing in from one joint directly flows out from the other joint when the second joint 130 is close to the first joint 120 can be avoided.

In some embodiments, the reservoir 100 may further comprise a plurality of housings 110. As shown in fig. 10, the liquid storage device 100 may further include a plurality of housings 110, where each housing 110 is correspondingly provided with a first connector 120 and a second connector 130. The plurality of cases 110 are connected in sequence. For any adjacent two housings 110 of the plurality of housings 110, the second connector 130 on one housing 110 is connected to the first connector 120 on the next housing 110 by a connecting tube 160. Through the aforementioned liquid storage device 100 including the plurality of shells 110, the volume of the liquid storage can be increased by multiple times, so as to meet the requirement of large capacity.

Finally, it should be understood that the embodiments described in this specification are merely illustrative of the principles of the embodiments of this specification. Other variations are possible within the scope of this description. Thus, by way of example, and not limitation, alternative configurations of embodiments of the present specification may be considered as consistent with the teachings of the present specification. Accordingly, the embodiments of the present specification are not limited to only the embodiments explicitly described and depicted in the present specification.

Claims

1. A fluid storage device, comprising:

a shell with an inner cavity, wherein a groove is formed in the inner side surface of the bottom wall of the shell;

the first connector is arranged on the shell and is provided with a first channel communicated with the inner cavity;

the inner tube is arranged in the inner cavity, one end of the inner tube is communicated with the first channel in the first joint, and the other end of the inner tube extends into the groove.

2. A reservoir according to claim 1, wherein the recess comprises a stepped recess formation, the bottom surface of the recess being recessed to form a sub-recess, the other end of the inner tube extending into the sub-recess.

3. The fluid reservoir device of claim 1, further comprising:

the second connector is arranged on the shell and is provided with a second channel communicated with the inner cavity.

4. A liquid storage device as defined in claim 3, wherein the housing further includes a side wall and a top wall, the bottom wall and the top wall being located at a lower end and an upper end of the side wall in a vertical direction of the housing, respectively, and the second connector and the first connector are both disposed on the top wall.

5. The fluid reservoir of claim 4, wherein the interior cavity has a baffle, a bottom edge of the baffle being connected to the bottom wall, and the baffle being located between projection positions of the second connector and the first connector on the bottom wall in a vertical direction of the housing, respectively; a gap is formed between a portion of at least one side edge of the partition plate, which is close to the bottom edge, and the side wall of the shell.

6. The fluid reservoir of claim 4, wherein the top wall comprises a first region and a second region, wherein the first region is spaced from the bottom wall a greater distance than the second region is spaced from the bottom wall.

7. The fluid reservoir of claim 6, wherein the second connector and the first connector are disposed on the second region.

8. A fluid reservoir according to claim 3, wherein the second connector and the first connector are detachably sealed connectors.

9. A fluid storage device as defined in claim 3, wherein the second connector comprises a luer connector, the fluid storage device further comprising a luer plug mated with the luer connector, the luer plug having a waterproof, breathable membrane disposed thereon.

10. A fluid reservoir according to claim 3, wherein the number of housings is plural, each housing having a corresponding first connector and second connector, the plurality of housings being connected in sequence, a second connector on one housing being connected to the first connector on the next housing for any adjacent two of the plurality of housings.