CN219647299U - A high pressure homogenization jar for liquid chromatography column fills - Google Patents

A high pressure homogenization jar for liquid chromatography column fills Download PDF

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Publication number
CN219647299U
CN219647299U CN202321279895.8U CN202321279895U CN219647299U CN 219647299 U CN219647299 U CN 219647299U CN 202321279895 U CN202321279895 U CN 202321279895U CN 219647299 U CN219647299 U CN 219647299U
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liquid
tank
thick bamboo
tank body
jar body
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CN202321279895.8U
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孙国威
阮必武
穆宁
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Wuxi Galak Chromatographic Technology Co ltd
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Wuxi Galak Chromatographic Technology Co ltd
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Abstract

A high-pressure homogenizing tank for filling a liquid chromatographic column, which relates to the technical field of liquid chromatographic column experimental equipment; the novel water tank comprises a tank body, wherein a first liquid outlet is formed in one end of the tank body, a switch valve is arranged at the first liquid outlet, the other end of the tank body is connected with an inlet pipe, a rotatable lantern ring is sleeved outside the inlet pipe, a sleeve is sleeved outside the lower part of the tank body, the tank body and the sleeve can rotate relatively, and a spiral groove is formed in the outer side of the tank body; including a cover section of thick bamboo, the one end of a cover section of thick bamboo is the opening setting, the opening internal diameter of a cover section of thick bamboo is unanimous with the diameter size of the jar body, a cover section of thick bamboo is inserted in the outside of the jar body from the top cover of the jar body through the opening of one end, be connected with the spring between the top of lantern ring and the top inboard of a cover section of thick bamboo, the inside coaxial coupling of feed inlet pipe has the inlet pipe, the one end of inlet pipe extends to the feed inlet pipe outside and is equipped with the feed inlet, feed inlet department is connected with the end cover that can fold the lid, the other end of inlet pipe runs through stirring part and the inside intercommunication of jar body.

Description

A high pressure homogenization jar for liquid chromatography column fills
Technical Field
The utility model relates to the technical field of liquid chromatographic column experimental equipment, in particular to a high-pressure homogenizing tank for filling a liquid chromatographic column.
Background
The current method for filling the liquid chromatographic column mainly comprises two methods of a dry method and a wet method: for fillers with larger particle size, dry packing can be used; while chromatographic packing of 3-10 μm is used for analysis, wet packing is often employed. Wet packing is also known as high pressure homogenization, i.e., a liquid chromatography column is prepared by first suspending the chromatography packing in a suitable homogenate to make a homogenate, and then pressing it into the column tube with a high pressure pump at a high flow rate immediately before it has settled.
In liquid chromatography column preparation, the stationary phase of the packing must be uniformly distributed in the column to ensure that the sample is able to pass uniformly through the packing, achieving efficient separation, and to achieve this objective it is often necessary to mix the packing and mobile phase uniformly using a homogenization tank.
The homogenization tanks for liquid chromatography column packing are typically made of metal or glass materials and can have a capacity of between a few milliliters and a few liters, and before packing the column, the stationary phase is typically prepared in advance to the desired size and added to the homogenization tank, and then the mobile phase and other additives are added for mixing, and the mixture is stirred in the homogenization tank until it is uniformly mixed.
The publication number is: the utility model of CN215727943U relates to a high-pressure homogenizing tank for filling a liquid chromatographic column, which comprises a homogenizing tank body, wherein one end of the homogenizing tank body is a liquid inlet end, the other end of the homogenizing tank body is a liquid outlet end, and both ends of the homogenizing tank body are provided with external threads; the inlet end department sets up the entry connects, the inside that the entry connects sets up columniform gondola water faucet, the gondola water faucet downwardly extending to inside the homogenization jar body, the up end opening of gondola water faucet, the opening to the inside axial extension of gondola water faucet just does not run through, the bottom of gondola water faucet is followed the radial of gondola water faucet sets up a plurality of round holes, the round hole with the opening intercommunication. The relative standard deviation of the column effect of the C18 silica gel chromatographic column adopting the high-pressure homogenizing tank is less than 5%, the repeatability is very good, the sealing pressure can reach 100MPa, the service life of the sealing element is greatly prolonged, and the replacement is very convenient.
In the prior art, the impact of the liquid with pressure fluctuation on the homogenate in the homogenate tank is solved, the pressure non-uniformity phenomenon of the homogenate is avoided, however, a stirring structure is not arranged in the homogenate tank, the fixed phase and the homogenate are not fully stirred, and the post-loading is affected, so the high-pressure homogenate tank for filling the liquid chromatographic column is provided for solving the problem.
Disclosure of Invention
The present utility model aims to solve the technical problems described above in the prior art.
The aim and the effect of the utility model are achieved by the following specific technical means:
the high-pressure homogenizing tank for filling the liquid chromatographic column comprises a tank body, wherein a first liquid outlet is formed in one end of the tank body, a switch valve is arranged at the first liquid outlet, the other end of the tank body is connected with an inlet pipe, a rotatable lantern ring is sleeved outside the inlet pipe, a sleeve is sleeved outside the lower part of the tank body, the tank body and the sleeve can rotate relatively, and a spiral groove is formed in the outer side of the tank body;
including the cover section of thick bamboo, the one end of cover section of thick bamboo is the opening setting, the opening internal diameter of cover section of thick bamboo is unanimous with the diameter size of the jar body, the cover section of thick bamboo is overlapped from the top of the jar body outside of the jar body through the opening of one end, be connected with the spring between the top of lantern ring and the top inboard of cover section of thick bamboo, and the ball that can 360 degrees rotations is installed to the opening inboard of cover section of thick bamboo, the ball outside slides in the helicla flute, the cover section of thick bamboo is kept away from open-ended one end an organic whole and is connected with the feed liquor pipe, the one end of feed liquor pipe connection cover section of thick bamboo extends to the cover section of thick bamboo inside and inserts the jar body from the import pipe, and the inner wall of import pipe is hugged closely in the outside of feed liquor pipe, the one end that the feed liquor pipe is located the inside one end of jar body is connected with stirring part, the one end outside of feed liquor pipe connection stirring part is equipped with the feed liquor mouth, the inside coaxial direction of inside of feed liquor pipe is connected with the inlet, one end of feed liquor pipe extends to the feed liquor pipe outside and is equipped with the feed inlet, the end cover that can fold lid is connected with.
Preferably, the switch valve is a glass switch valve.
Preferably, the sleeve and the tank body are mounted through bearings.
Preferably, the stirring part comprises a stirring head, a groove, a top plate, a through hole, a liquid outlet and a convex strip, wherein the end part of the liquid inlet pipe 4 is fixedly connected with the stirring head, the stirring head is in an inverted truncated cone shape, the stirring head is connected with one end outer side of the liquid inlet pipe and is in fit sliding with the inner wall of the tank body, the top of the stirring head is provided with the groove, the top plate with the through hole is fixed at the opening of the groove, and the liquid outlets of a plurality of groups of communicated tank bodies are circumferentially arranged below the inner wall of the groove.
Preferably, the outer circumference of the stirring head is sequentially connected with convex strips.
Preferably, the liquid outlet is horizontally arranged corresponding to the inner wall of the tank body.
Preferably, the liquid outlet is obliquely arranged corresponding to the inner wall of the tank body, and the included angle between the liquid outlet and the inner wall of the tank body is 10-75 degrees.
Preferably, the rubber bulge is arranged on one side of the end cover covering the feeding pipe.
The beneficial effects are that:
1. the high-pressure homogenizing tank for filling the liquid chromatographic column is provided with the cover cylinder, the cover cylinder can drive the stirring head of the stirring component to squeeze filler and homogenate when moving downwards, and simultaneously the cover cylinder can drive the tank body to rotate by sliding the balls in the spiral groove when moving downwards, so that the homogenate and the filler are uniformly stirred by the convex strips outside the stirring head.
2. This kind of a high pressure homogenate jar for liquid chromatography column fills is provided with stirring element, the replacement liquid that the feed liquor pipe got into is discharged from the second liquid outlet, in entering the recess through the through-hole, finally discharge into the jar body through the leakage fluid dram, because the leakage fluid dram sets up along recess inner wall circumference, the replacement liquid that extrudes through pressure is not direct with the homogenate collision, but gets into the recess behind the roof earlier, then spout the inner wall to jar body from the leakage fluid dram, flow into jar body 1 in along the inner wall of jar body downwards for homogenate in jar body 1 can be stable, is difficult for causing the impact, has improved the homogeneity of chromatographic column bed.
Drawings
FIG. 1 is a schematic diagram of a high pressure homogenization tank for liquid chromatography column packing in accordance with the present utility model.
FIG. 2 is a schematic cross-sectional view of a high pressure homogenization tank for liquid chromatography column packing in accordance with the present utility model.
FIG. 3 is a schematic view of the stirring member of the present utility model.
FIG. 4 is a schematic cross-sectional view of a stirring element according to the present utility model.
In fig. 1-4: the tank body 1, the first liquid outlet 101, the inlet pipe 102, the sleeve 2, the cover cylinder 3, the liquid inlet pipe 4, the liquid inlet 401, the second liquid outlet 402, the feed pipe 5, the spring 6, the stirring part 7, the stirring head 701, the groove 702, the top plate 703, the through hole 704, the liquid outlet 705, the convex strip 706, the ball 8, the spiral groove 9, the lantern ring 10, the switch valve 11 and the end cover 12.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
Referring to fig. 1-4, a high-pressure homogenizing tank for filling a liquid chromatographic column comprises a tank body 1, wherein a first liquid outlet 101 is formed in one end of the tank body 1, a switch valve 11 is installed at the first liquid outlet 101, the switch valve 11 is used for controlling the switch of the first liquid outlet 101, a glass switch valve is selected as the switch valve 11, an inlet pipe 102 is connected to the other end of the tank body 1, a lantern ring 10 is sleeved outside the inlet pipe 102, the lantern ring 10 is installed outside the inlet pipe 102 through a bearing and can rotate, a sleeve 2 is sleeved outside the lower part of the tank body 1, the sleeve 2 and the tank body 1 are installed through a bearing, the tank body 1 and the sleeve 2 can rotate relatively, when the tank body 1 is installed, the sleeve 2 is clamped and fixed by a clamping tool, and the tank body 1 can rotate by external force;
the outer side of the tank body 1 is provided with a spiral groove 9;
the device comprises a cover cylinder 3, wherein one end of the cover cylinder 3 is provided with an opening, the opening inner diameter of the cover cylinder 3 is consistent with the diameter of a tank body 1, the cover cylinder 3 is sleeved and inserted on the outer side of the tank body 1 from the upper side of the tank body 1 through the opening at one end, a ball 8 capable of rotating by 360 degrees is arranged on the inner side of the opening of the cover cylinder 3, the outer side of the ball 8 slides in a spiral groove 9, one end of the cover cylinder 3 far away from the opening is integrally connected with a liquid inlet pipe 4, one end of the liquid inlet pipe 4 connected with the cover cylinder 3 extends into the cover cylinder 3 and is inserted into the tank body 1 from an inlet pipe 102, the outer side of the liquid inlet pipe 4 is tightly attached to the inner wall of the inlet pipe 102, one end of the liquid inlet pipe 4 positioned in the tank body 1 is connected with a stirring part 7, one end outer side of the liquid inlet pipe 4 connected with the stirring part 7 is provided with a plurality of groups of second liquid outlets 402, one side of the outer side of the liquid inlet pipe 4 positioned in the cover cylinder 3 is connected with a liquid inlet 401, and slurry is added from the liquid inlet 401 into the tank body 1 through the liquid inlet pipe 4;
the inside of feed liquor pipe 4 is with axial connection there being inlet pipe 5, the one end of inlet pipe 5 extends to the feed liquor pipe 4 outside and is equipped with the feed inlet, stirring part 7 is run through to the other end of inlet pipe 5 and the inside intercommunication of jar body 1, can add the inside of jar body 1 with the filler through the feed inlet of inlet pipe 5, be connected with the end cover 12 that can fold the lid and close in feed inlet department, end cover 12 lid closes inlet pipe 5 one side and is the rubber arch, the lid closes can seal the feed inlet behind the feed inlet, be connected with spring 6 between the top of lantern ring 10 and the top inboard of cover section of thick bamboo 3, when extrusion cover section of thick bamboo 3 down moves along jar body 1, then move in helical groove 9 through ball 8, can drive jar body 1 rotation, stirring part 7 then can stir and extrude the homogenate in the jar body 1 with the filler, reach the even effect of compounding.
During the use, fix sleeve 2 through centre gripping frock, open end cover 12 and lead into jar body 1 through inlet pipe 5 with the packing, then cover the feed inlet of inlet pipe 5 with end cover 12, add the homogenate through inlet 401, get into jar body through feed pipe 4, if packing and homogenate have preliminary supersound homogenate had, can lead into jar body 1 through inlet pipe 5 with the homogenate mixed liquor of preliminary homogenate, then promote the cover cylinder 3 and move down, move in helical groove 9 through ball 8, can drive jar body 1 rotation, stirring part 7 then can stir and extrude the homogenate and packing in jar body 1, after the homogenate is accomplished, loosen cover cylinder 3, spring 6 reaction force promotes cover cylinder 3 and resets, communicate chromatographic column and first liquid outlet 101 and fixed screw thread installation are fixed, open switch valve 11, then add the replacement liquid to jar body 1 through inlet 401, under the pressure that has been adjusted in advance, with the replacement liquid input jar body 1, wait to get into the chromatographic column in the chromatographic column through jar body 1, after the chromatographic column is gone out, load out of chromatographic column is cooled down to the chromatographic column in the pressure machine, when waiting for 20 minutes, load down to stand still, the chromatographic column is cooled down, the pressure system is cooled down, the normal pressure is kept down, the chromatographic column is cooled down, after the pressure is kept down, the chromatographic column is loaded down, and the chromatography is moved down, and the normal pressure is left down, and is moved.
In this embodiment, the stirring member 7 includes a stirring head 701, a groove 702, a top plate 703, a through hole 704, a liquid drain port 705 and a raised line 706, the stirring head 701 is in an inverted truncated cone shape, the end of the liquid inlet pipe 4 is fixedly connected with the stirring head 701, the outer side of one end of the stirring head 701 connected with the liquid inlet pipe 4 is in fit sliding with the inner wall of the tank 1, the top of the stirring head 701 is provided with the groove 702, the top plate 703 with the through hole 704 is fixed at the opening of the groove 702, a plurality of groups of liquid drain ports 705 communicated with the tank 1 are circumferentially arranged below the inner wall of the groove 702, it is understood that the displacement liquid entering the liquid inlet pipe 4 is discharged from the second liquid outlet 402, enters the groove 702 through the through hole 704, and finally is discharged into the tank through the liquid drain port 705, because the liquid drain port 705 is circumferentially arranged along the inner wall of the groove 702, the displacement liquid extruded through pressure is not directly collided with the homogenate, but firstly enters the groove 702 after passing through the top plate 705, then flows from the liquid drain port 705 to the inner wall of the tank 1, flows downwards into the tank 1 along the inner wall of the tank 1, so that the homogenate liquid in the tank 1 is easily discharged from the inner wall, the second liquid outlet 402, the liquid is able to be stable, the column is impacted by the column, and the column is not stable, and the column is improved, and the column uniformity is improved.
In this embodiment, the protruding strips 706 are sequentially connected to the outer circumference of the stirring head 701, so that the stirring head 701 can squeeze the filler and the homogenate while moving downward, and the protruding strips 706 can facilitate the mixing of the filler and the homogenate.
In this embodiment, the liquid draining port 705 may be horizontally arranged or obliquely arranged, and if the liquid draining port is at an inclination angle, the included angle between the liquid draining port 705 and the inner wall of the tank body 1 is 10-75 °, and no matter horizontally arranged or obliquely arranged, the displacement liquid sprayed out by the liquid draining port 705 is in contact with the inner wall of the tank body 1.
Working principle:
when in use, the sleeve 2 is fixed by the clamping tool, the end cover 12 is opened to guide filler into the tank body 1 through the feed pipe 5, then the end cover 12 is covered with the feed inlet of the feed pipe 5, homogenate is added through the feed inlet 401, passes through the second liquid outlet 402 of the liquid inlet pipe 4, then enters the groove 702 through the through hole 704, finally enters the tank body 1 through the liquid outlet 705, if the filler and the homogenate are subjected to preliminary ultrasonic homogenate, the homogenate mixed liquid of the preliminary homogenate can be guided into the tank body 1 through the feed pipe 5, then the cover cylinder 3 is pushed to move downwards, the ball 8 moves in the spiral groove 9 to drive the tank body 1 to rotate, the stirring part 7 can stir and squeeze the homogenate and the filler in the tank body 1, after the homogenate is completed, the cover cylinder 3 is released, the reaction force of the spring 6 pushes the cover cylinder 3 to reset, the chromatographic column is communicated with the first liquid outlet 101 and is fixedly arranged by the fixed screw threads, the switch valve 11 is opened, then the displacement liquid is added into the tank body 1 through the liquid inlet 401, the displacement liquid entering through the liquid inlet pipe 4 is discharged from the second liquid outlet 402, enters the groove 702 through the through hole 704, finally enters the tank body through the liquid outlet 705, the displacement liquid extruded by pressure is not directly collided with the homogenate liquid, but firstly enters the groove 702 through the top plate 703, then is sprayed to the inner wall of the tank body 1 from the liquid outlet 705, flows downwards into the tank body 1 along the inner wall of the tank body 1, so that the homogenate liquid in the tank body 1 can be stable and is not easy to cause impact, the uniformity of a chromatographic column bed is improved, a column filling machine is started, the displacement liquid is input into the tank body 1 under the pressure which is regulated in advance, enters the chromatographic column through the tank body 1, after the displacement liquid flows out of the chromatographic column, the column filling machine is closed, the pressurization is stopped until the system pressure is reduced to normal pressure, standing for 20-40 min, and discharging the chromatographic column.

Claims (8)

1. The utility model provides a high pressure homogenization jar for liquid chromatography post fills, includes a jar body (1), its characterized in that, the one end of jar body (1) is equipped with first liquid outlet (101), and on-off valve (11) are installed to first liquid outlet (101) department, the other end of jar body (1) is connected with inlet tube (102), the cover is established in the outside of inlet tube (102) and is installed lantern ring (10) that can rotate, sleeve (2) are installed in the outside cover of the below of jar body (1), and jar body (1) and sleeve (2) can relative rotation, and the outside of jar body (1) is equipped with helical groove (9);
including cover section of thick bamboo (3), the one end of cover section of thick bamboo (3) is the opening setting, and the opening internal diameter of cover section of thick bamboo (3) is unanimous with the diameter size of jar body (1), and cover section of thick bamboo (3) is inserted in the outside of jar body (1) from the top cover of jar body (1) through the opening of one end, be connected with spring (6) between the top of lantern ring (10) and the top inboard of cover section of thick bamboo (3), and install ball (8) that can 360 degrees rotations in the opening inboard of cover section of thick bamboo (3), ball (8) outside slides in helical groove (9), the one end an organic whole that keeps away from the opening of cover section of thick bamboo (3) is connected with feed liquor pipe (4), and feed liquor pipe (4) connect the one end of cover section of thick bamboo (3) and extend to the inside of cover section of thick bamboo (3) and insert in jar body (1) from inlet tube (102), and the outside of feed liquor pipe (4) is hugged closely the inner wall of inlet tube (102), and feed liquor pipe (4) are located the inside one end of jar body (1) and are connected with stirring part (7), and feed liquor pipe (4) are connected with multiunit (5) feed liquor pipe (4) outside (5) and are located outside feed liquor pipe (4), one end of the feed pipe (5) extends to the outside of the liquid inlet pipe (4) and is provided with a feed inlet, an end cover (12) which can be folded and covered is connected to the feed inlet, and the other end of the feed pipe (5) penetrates through the stirring part (7) and is communicated with the inside of the tank body (1).
2. The high-pressure homogenization tank for liquid chromatography column packing according to claim 1, characterized in that the on-off valve (11) is a glass on-off valve.
3. A high pressure homogenization tank for liquid chromatography column packing according to claim 1, characterized in that the sleeve (2) is mounted with bearings to the tank body (1).
4. The high-pressure homogenizing tank for filling the liquid chromatographic column according to claim 1, wherein the stirring component (7) comprises a stirring head (701), a groove (702), a top plate (703), a through hole (704), a liquid outlet (705) and a raised strip (706), the end part of the liquid inlet pipe (4) is fixedly connected with the stirring head (701), the stirring head (701) is in an inverted truncated cone shape, the outer side of one end of the stirring head (701) connected with the liquid inlet pipe (4) is attached and slides with the inner wall of the tank body (1), the top of the stirring head (701) is provided with the groove (702), the top plate (703) with the through hole (704) is fixed at the opening of the groove (702), and a plurality of groups of liquid outlets (705) communicated with the tank body (1) are circumferentially arranged below the inner wall of the groove (702).
5. The high-pressure homogenization tank for liquid chromatography column packing according to claim 4, wherein the outside circumference of the stirring head (701) is sequentially connected with convex strips (706).
6. The high-pressure homogenizing tank for liquid chromatography column packing as claimed in claim 4, wherein the liquid discharge port (705) is provided horizontally corresponding to the inner wall of the tank body (1).
7. The high-pressure homogenizing tank for filling a liquid chromatographic column as claimed in claim 4, wherein the liquid discharge port (705) is arranged obliquely corresponding to the inner wall of the tank body (1), and the liquid discharge port (705) has an included angle of 10-75 degrees with the inner wall of the tank body (1).
8. The high-pressure homogenizing tank for filling a liquid chromatography column according to claim 1, wherein the side of the end cover (12) covering the feed pipe (5) is a rubber protrusion.
CN202321279895.8U 2023-05-25 2023-05-25 A high pressure homogenization jar for liquid chromatography column fills Active CN219647299U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202321279895.8U CN219647299U (en) 2023-05-25 2023-05-25 A high pressure homogenization jar for liquid chromatography column fills

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202321279895.8U CN219647299U (en) 2023-05-25 2023-05-25 A high pressure homogenization jar for liquid chromatography column fills

Publications (1)

Publication Number Publication Date
CN219647299U true CN219647299U (en) 2023-09-08

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117233303A (en) * 2023-11-13 2023-12-15 维慕曦生物科技(常州)有限公司 Automatic filling device for liquid chromatographic column

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117233303A (en) * 2023-11-13 2023-12-15 维慕曦生物科技(常州)有限公司 Automatic filling device for liquid chromatographic column
CN117233303B (en) * 2023-11-13 2024-01-19 维慕曦生物科技(常州)有限公司 Automatic filling device for liquid chromatographic column

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