CN219574124U

CN219574124U - HIV-1/2 type new-born infection detection kit

Info

Publication number: CN219574124U
Application number: CN202223278446.XU
Authority: CN
Inventors: 方会来
Original assignee: Tianjin Bremen Bioengineering Co ltd
Current assignee: Tianjin Bremen Bioengineering Co ltd
Priority date: 2022-12-07
Filing date: 2022-12-07
Publication date: 2023-08-22
Anticipated expiration: 2032-12-07

Abstract

The utility model relates to an HIV-1/2 type new infection detection kit, which comprises a kit tank, a base, diluent and colloidal gold; the reagent tank is a cylindrical hollow tank body, and the top surface of the reagent tank is opened and is provided with a cover body in a matched manner; the upper part of the peripheral side of the reagent tank is provided with a first external thread, and the lower part of the peripheral side of the reagent tank is provided with a second external thread; the bottom surface of the reagent tank is provided with an opening and a layer of film; the base is in a round table shape, a round groove with an upper opening is formed in the top surface of the base, an inner thread is arranged on the inner side wall of the round groove, the inner thread is matched with the first outer thread, and the inner thread is matched with the second outer thread; the inner bottom of the round groove is provided with a puncture tip bottom, a layer of liquid storage layer and a layer of detection reagent film. According to the utility model, the detection reagent films arranged on the reagent tank and the base are connected into a whole through threaded connection, so that the operation of detection personnel is greatly facilitated, and the detection accuracy is ensured.

Description

HIV-1/2 type new-born infection detection kit

Technical Field

The utility model relates to the technical field of kits, in particular to an HIV-1/2 new-born infection detection kit.

Background

In the prior art, an HIV detection kit using a spot gold immunofiltration assay generally comprises a filtration card, a reagent bottle, a diluent, colloidal gold, etc., the reagent bottle and the filtration card are sealed in an aluminum foil bag, as shown in the figure, the reagent bottle is provided with a bottle stopper, the bottle stopper is plugged by a buckle in the reagent bottle, a plurality of reagent bottles are connected by a plastic clamping plate, the plastic clamping plate is provided with a circular ring, the circular ring can be sleeved on the bottle body of the reagent bottle, and also can be sleeved on the bottle stopper, and the connecting point of the plastic clamping plate between two reagent bottles can be broken.

An 8-person HIV detection kit generally comprises 8 reagent bottles and 8 percolation cards, wherein the 8 reagent bottles are connected through plastic clamping plates, the percolation cards and the reagent bottles are not connected, and when HIV detection is carried out, colloidal gold, diluent, serum or plasma samples are required to be added into the reagent bottles for fully and uniformly mixing reaction, and then the mixed solution is added into the percolation cards for reaction. The technical problems are that: the percolation cards used for each detection sample are in one-to-one correspondence with the reagent bottles, and marks are respectively made for distinguishing, but the reagent bottles are small in size and inconvenient to make.

According to the quick HIV antibody confirmation detection kit disclosed by the utility model, application number 202121737078.3 is that a reagent bottle is connected with a percolation card in a matched manner, each reagent bottle is connected with an independent percolation card in a matched manner, a label can be attached to the percolation card with a relatively large volume when the reagent bottle is used, after liquid is treated in the reagent bottle, the percolation card is torn off for subsequent operation, the problems of inconvenient identification on the reagent bottle and inconvenience in one-to-one operation of the reagent bottle and the percolation card of each detection sample are solved, and the detection kit is convenient for operation of detection staff. The solution adopted by the utility model is different from the solution adopted by the utility model.

The HIV-1/2 new infection detection kit is designed to solve the problem that the reagent bottles are inconvenient to operate when the reagent bottles of each detection sample are in one-to-one correspondence with the percolation cards.

Disclosure of Invention

The utility model provides an HIV-1/2 new infection detection kit for overcoming the defects, which comprises a kit tank, a base, a diluent and colloidal gold;

the reagent tank is a cylindrical hollow tank body, and the top surface of the reagent tank is opened and is provided with a cover body in a matched manner; the upper part of the peripheral side of the reagent tank is provided with a first external thread, and the lower part of the peripheral side of the reagent tank is provided with a second external thread; the bottom surface of the reagent tank is provided with an opening and a layer of film;

the base is in a round table shape, a round groove with an upper opening is formed in the top surface of the base, an inner thread is arranged on the inner side wall of the round groove, the inner thread is matched with the first outer thread, and the inner thread is matched with the second outer thread;

the inner bottom of the round groove is provided with a puncture tip bottom, a liquid storage layer and a detection reagent film, the liquid storage layer is positioned below the puncture tip bottom, and the liquid storage layer is positioned below the detection reagent film; the puncture tip bottom is in a tip bottom shape with a protruding top, and the middle part of the puncture tip bottom protrudes upwards to form a tip for puncturing the film; the puncture tip bottom is provided with a liquid inlet hole, and the liquid inlet Kong Wei is arranged on the periphery of the tip bottom;

the detection reagent film is provided with a first detection line for coating HIV recombinant antigen gp, a second detection line for coating HIV recombinant antigen gp+gp, a third detection line for coating HIV recombinant antigen gp and a quality control line for coating sheep anti-rabbit IgG;

preferably, the colloidal gold is a colloidal gold labeled with staphylococcus aureus protein A.

Preferably, the puncture tip bottom is made of stainless steel.

Preferably, the film is made of thin aluminum.

Preferably, the cover body is a plug body, and the opening of the reagent tank is plugged by a buckle arranged in the reagent tank.

Preferably, the detection reagent membrane is an osmosis membrane.

Preferably, the liquid storage layer is one or two of a water absorption pad and a drying agent.

The beneficial effects of the utility model are as follows:

the HIV-1/2 type new infection detection kit disclosed by the utility model is characterized in that the reagent tank and the detection reagent film arranged on the base are connected into a whole through threaded connection, the reagent tank and the detection reagent film can be integrated in both liquid adding and reaction, only the base is required to be marked, the reaction on the reagent tank with small volume is not required, the sample is not mistaken, the operation of a detector is greatly facilitated, and the detection accuracy is ensured.

Drawings

FIG. 1 is a perspective view of a reservoir;

FIG. 2 is a cross-sectional view of FIG. 1;

FIG. 3 is a perspective view of a detection base;

FIG. 4 is a cross-sectional view of the present utility model in a deployed state;

FIG. 5 is a cross-sectional view of the present utility model in use;

wherein:

1-a reagent tank; 11-first thread; 12-second thread; 2-a cover; 3-film; 31-a circular groove; 4-puncturing the tip bottom; 5-detecting reagent film; a 6-memory layer; 7-a base; 8-a liquid inlet hole;

Detailed Description

The embodiments of the present utility model will be described in detail below with reference to the accompanying drawings.

The HIV-1/2 new infection detection kit comprises a kit tank 1, a base 7, diluent and colloidal gold;

as shown in fig. 1 and 2, the reagent tank 1 is a cylindrical hollow tank body, and a cover body 2 is provided on the top surface of the reagent tank 1 in a matching manner; a first external thread 11 is formed at the upper part of the circumference side of the reagent tank 1, and a second external thread 12 is formed at the lower part of the circumference side of the reagent tank 1; the bottom surface of the reagent tank 1 is open and provided with a layer of film 3;

as shown in fig. 3, the base 7 is in a shape of a circular table, a circular groove 31 with an upper opening is formed in the top surface of the base 7, an internal thread is formed in the inner side wall of the circular groove 31, the internal thread is matched with the first external thread 11, and the internal thread is matched with the second external thread 12;

the inner bottom of the round groove 31 is provided with a puncture tip 4, a liquid storage layer 6 and a detection reagent film 5, the liquid storage layer 6 is positioned below the puncture tip 4, and the liquid storage layer 6 is positioned below the detection reagent film 5; the puncture tip bottom 4 is in a tip bottom shape with a protruding top, and the middle part of the puncture tip bottom 4 protrudes upwards to form a tip for puncturing the film 3; the puncture tip bottom 4 is provided with a liquid inlet hole 8, and the liquid inlet hole 8 is surrounded on the periphery of the puncture tip bottom 4;

the detection reagent film 5 is provided with a first detection line for coating HIV recombinant antigen gp36, a second detection line for coating HIV recombinant antigen gp36+gp41, a third detection line for coating HIV recombinant antigen gp160 and a quality control line for coating sheep anti-rabbit IgG, the period range of the antigen gp160 is wider than that of the antigen gp36 and gp41 detection method at present, and gp160 is used as a mark, so that antibodies except for IgG types can be captured, and the occurrence of the omission phenomenon is reduced;

Preferably, the puncture tip bottom 4 is made of stainless steel.

Preferably, the film 3 is made of thin aluminum.

Preferably, the cover body 2 is a plug body, and the plug body is tightly plugged at the tank opening of the reagent tank 1 through a buckle arranged in the reagent tank 1, so that the effects of moisture prevention and leakage prevention can be achieved.

Preferably, the detection reagent film 5 is an osmosis film.

Preferably, the storage layer 6 is one or both of a water absorbing pad and a desiccant.

The using method comprises the following steps:

when the HIV is detected, the cover 2 is opened, colloidal gold, diluent, serum or plasma sample is added into the reagent tank 1, the cover 2 is covered, as shown in fig. 4, one end of the cover 2 is connected with the internal thread on the circular groove 31 downwards through the external thread 11, fully and uniformly mixed, the reagent tank 1 is unscrewed to punch the cover 2, the external thread 12 is connected with the internal thread of the circular groove 31, at this time, after the membrane 3 is punctured by the puncture tip 4, the mixed solution flows into the detection reagent film 5 from the liquid inlet 8 on the puncture tip 4 to react, and the redundant liquid is absorbed and stored on the liquid storage layer 6.

The detection reagent film 5 is provided with a first detection line for coating HIV recombinant antigen gp36, a second detection line for coating HIV recombinant antigen gp36+gp41, a third detection line for coating HIV recombinant antigen gp160 and a quality control line for coating sheep anti-rabbit IgG;

when checking the detection result, no matter whether the serum/plasma to be detected has HIV antibody or not, the quality control line can turn into purple, otherwise, the operation error or reagent failure is indicated, and when the serum/plasma to be detected contains HIV antibody, the corresponding detection line on the detection reagent film 5 can turn into purple.

In the description of the present utility model, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present utility model and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art in a specific case.

The foregoing is merely a preferred embodiment of the present utility model and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present utility model, which are intended to be comprehended within the scope of the present utility model.

Claims

HIV-1/2 new infection detection kit, including reagent tank (1) and base (7), diluent, colloidal gold;

the reagent tank is characterized in that the reagent tank (1) is a cylindrical hollow tank body, and the top surface of the reagent tank (1) is opened and is provided with a cover body (2) in a matching way; the upper part of the circumference of the reagent tank (1) is provided with a number one external thread (11), and the lower part of the circumference of the reagent tank (1) is provided with a number two external thread (12); the bottom surface of the reagent tank (1) is open and provided with a layer of film (3);

the base (7) is in a round table shape, a round groove (31) with an upper opening is formed in the top surface of the base (7), an inner thread is arranged on the inner side wall of the round groove (31), the inner thread is matched with the first external thread (11), and the inner thread is matched with the second external thread (12);

the inner bottom of the round groove (31) is provided with a puncture pointed bottom (4), a layer of liquid storage layer (6) and a layer of detection reagent film (5), the liquid storage layer (6) is positioned below the puncture pointed bottom (4), and the liquid storage layer (6) is positioned below the detection reagent film (5); the puncture tip bottom (4) is in a tip bottom shape with a protruding top, and the middle part of the puncture tip bottom (4) protrudes upwards to form a tip for puncturing the film (3); the puncture tip bottom (4) is provided with a liquid inlet hole (8), and the liquid inlet hole (8) is surrounded on the periphery of the tip bottom (4);

the detection reagent film (5) is provided with a first detection line for coating HIV recombinant antigen gp36, a second detection line for coating HIV recombinant antigen gp36+gp41, a third detection line for coating HIV recombinant antigen gp160 and a quality control line for coating sheep anti-rabbit IgG.
2. The kit for detecting HIV-1/2 infection according to claim 1, wherein the colloidal gold is a colloidal gold labeled with Staphylococcus aureus protein A.
3. The HIV-1/2 type new infection detection kit according to claim 2, wherein the puncture tip bottom (4) is made of stainless steel.
4. The kit for detecting HIV-1/2 infection according to claim 3, wherein the film (3) is made of a thin aluminum material.
5. The HIV-1/2 type new infection detection kit according to claim 4, wherein the cover body (2) is a plug body, and the plug is tightly plugged at the tank opening of the reagent tank (1) through a buckle arranged in the reagent tank (1).
6. The HIV-1/2 type new infection detection kit according to claim 5, wherein the detection reagent membrane (5) is a diafiltration membrane.
7. The HIV-1/2 type new infection detection kit according to claim 6, wherein the reservoir layer (6) is one or both of a water absorbing pad or a desiccant.