CN219546965U - Efficient monocyte extraction centrifuge tube - Google Patents

Abstract

The utility model discloses a monocyte efficient extraction centrifuge tube, which comprises a tube body, wherein a sealing sleeve is arranged above the tube body, a hollow groove is formed in the lower wall of the sealing sleeve, an air bag is arranged in the hollow groove, a sealing piston is fixedly connected below the air bag, and the sealing piston is in sealing sliding fit with the inner wall of the tube body; the pushing assembly comprises a cavity, a rotating shaft, a lantern ring, a connecting rod, a first pushing ball, an air inlet channel, an air storage chamber, a suction piston, a spring, a push rod and a second pushing ball. According to the utility model, through arranging the pushing component, the suction piston can suck outside air back and forth by the inertia force generated when the centrifugal tube rotates, when the mononuclear cell layer is extracted, the control valve is opened, so that high-pressure gas can enter the air bag, the air bag is expanded to push the sealing piston downwards, the mononuclear cell layer is rapidly discharged, and the extraction efficiency of the mononuclear cell layer is greatly improved.

Description

Efficient monocyte extraction centrifuge tube

Technical Field

The utility model relates to the technical field of centrifuge tubes, in particular to a monocyte efficient extraction centrifuge tube.

Background

Monocytes, which are the largest blood cells in blood and the largest volume of leukocytes, are an important component of the body's defense system, and when the body is inflamed or has other diseases, the percentage of total number of monocytes can be changed, so that checking the monocyte count becomes an important method for assisting diagnosis, and when checking the monocyte count, a centrifuge tube is often needed for separating and extracting the monocytes.

Through retrieval, the patent document with the publication number of CN210736756U discloses a centrifugal tube for cell extraction, which comprises a centrifugal tube main body, wherein the outer side of the centrifugal tube main body is provided with an outer tube wall, the inner side of the outer tube wall is provided with a liquid containing groove, the outer side of the outer tube wall is provided with scale marks, the top of the centrifugal tube main body is provided with a rubber ring, the right side of a connecting rod is provided with a centrifugal tube cover, and the inner side of the centrifugal tube cover is provided with a circular ring groove body; the Teflon support block is fixed at the center of the sealing structure through colloid bonding, the rubber filling plug is fixed at the outer ring of the Teflon support block through colloid bonding, and the hydrophobic veneer is fixed at the outer surface of the Teflon support block through colloid bonding; the anti-sticking material layer is welded and fixed on the inner wall of the supporting layer, and the wear-resisting layer is welded and fixed on the outer wall of the pipe bottom structure.

However, when the centrifuge tube needs to take out the mononuclear cell layer after centrifugation, the mononuclear cell layer can only be naturally discharged from the liquid outlet at the bottom of the tube under the action of gravity, and the extraction efficiency is often low.

Disclosure of Invention

The utility model aims to solve the problem of low extraction efficiency when the mononuclear cell layer is naturally discharged by utilizing the action of gravity in the prior art, and provides a high-efficiency extraction centrifuge tube capable of enabling a sealing piston to downwards displace by utilizing air pressure so as to rapidly discharge mononuclear cells of the mononuclear cell layer.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

monocyte high efficiency draws centrifuging tube, includes:

the sealing device comprises a pipe body, wherein a sealing sleeve is arranged above the pipe body, a hollow groove is formed in the lower wall of the sealing sleeve, an air bag is arranged in the hollow groove, a sealing piston is fixedly connected below the air bag, and the sealing piston is in sealing sliding fit with the inner wall of the pipe body;

the pushing assembly comprises a cavity, a rotating shaft, a lantern ring, a connecting rod, a first pushing ball, an air inlet channel, an air storage chamber, a suction piston, springs, a push rod and a second pushing ball, wherein the cavity is formed in the sealing sleeve, the rotating shaft is connected in a rotating mode in the cavity, the lantern ring is sleeved in the rotating shaft in a rotating mode, the connecting rod is fixedly connected to the lantern ring, the first pushing ball is fixedly connected to one side of the connecting rod, away from the lantern ring, of the sealing sleeve, the air inlet channel is formed in the sealing sleeve, the air storage chamber is formed in the sealing sleeve, one side of the air inlet channel is communicated with the outside, the other side of the air inlet channel is communicated with the air storage chamber, the suction piston is in sealing sliding fit with the suction piston in the air inlet channel, the suction piston is in elastic connection with the inner wall of the air inlet channel through two springs, and the push rod is fixedly connected to one side wall of the suction piston, and the push rod extends to the inside of the cavity, and the second pushing ball is fixedly connected to the push rod.

Preferably, a fixing sleeve is fixedly connected below the sealing sleeve, and the fixing sleeve can be fixed on the side wall of the pipe body.

Preferably, a one-way valve is arranged on one side of the air inlet channel close to the outside, and the one-way valve enables the outside air to flow into the air inlet channel only.

Preferably, the air reservoir communicates with the air bag interior space through an exhaust passage thereunder.

Preferably, a control valve is arranged in the exhaust passage.

Preferably, the air bag is provided with an exhaust valve.

Compared with the prior art, the utility model has the following advantages: according to the centrifugal tube, the pushing component is arranged, and the rotating speed of the centrifugal tube is usually high during rotation and centrifugation, so that the first pushing ball can be used for pushing the second pushing ball in a reciprocating mode through inertia force, further, the suction piston can be used for sucking air out of the periphery in a reciprocating mode, the air is stored in the air storage chamber, when the mononuclear cell layer is required to be extracted after centrifugation is completed, high-pressure air can enter the air bag only by opening the control valve, the air bag is inflated to push the sealing piston downwards, the mononuclear cell layer is discharged out rapidly, and the mononuclear cell layer extraction efficiency is greatly improved.

Drawings

FIG. 1 is a schematic diagram of a monocyte efficient extraction centrifuge tube according to the present utility model;

FIG. 2 is an enlarged view of FIG. 1 at A;

fig. 3 is an enlarged view at B in fig. 1.

In the figure: 1. a tube body; 11. sealing sleeve; 111. a fixed sleeve; 12. a hollow groove; 13. an air bag; 14. a sealing piston; 2. a pushing assembly; 21. a cavity; 22. a rotating shaft; 23. a collar; 24. a connecting rod; 25. a first pushing ball; 26. an air intake passage; 27. an air storage chamber; 28. a suction piston; 29. a spring; 210. a push rod; 211. a second pushing ball; 212. a one-way valve; 213. an exhaust passage; 214. and a control valve.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.

Referring to fig. 1-3, a monocyte high efficiency extraction centrifuge tube comprising:

the pipe body 1 is provided with a sealing sleeve 11 above the pipe body 1, the lower wall of the sealing sleeve 11 is provided with an empty groove 12, an air bag 13 is arranged in the empty groove 12, a sealing piston 14 is fixedly connected below the air bag 13, the sealing piston 14 is in sealing sliding fit with the inner wall of the pipe body 1, a fixing sleeve 111 is fixedly connected below the sealing sleeve 11, the fixing sleeve 111 can be fixed on the side wall of the pipe body 1, when mononuclear cells are centrifuged, the sealing sleeve 11 is fixed above the pipe body 1 through rotating the fixing sleeve 111, the pipe body 1 is subjected to sealing treatment, after extraction is completed, the sealing sleeve 11 can be taken down from the upper side of the pipe body 1 through reversely rotating the fixing sleeve 111, and the fixing mode of the fixing sleeve 111 is screw locking, so that the technology is not repeated;

the pushing assembly 2 comprises a cavity 21, a rotating shaft 22, a lantern ring 23, a connecting rod 24, a first pushing ball 25, an air inlet channel 26, an air storage chamber 27, a suction piston 28, a spring 29, a push rod 210 and a second pushing ball 211, wherein the cavity 21 is formed in the sealing sleeve 11, the rotating shaft 22 is connected with the rotating shaft 22 in an inward rotating mode, the lantern ring 23 is rotatably sleeved on the rotating shaft 22, the connecting rod 24 is fixedly connected with the lantern ring 23, one side, far away from the lantern ring 23, of the connecting rod 24 is fixedly connected with the first pushing ball 25, the air inlet channel 26 is formed in the sealing sleeve 11, the air storage chamber 27 is formed in the sealing sleeve 11 and is located below the air inlet channel 26, one side of the air inlet channel 26 is communicated with the outside, the other side of the air inlet channel 26 is communicated with the air storage chamber 27, the suction piston 28 is in sealing sliding fit in the air inlet channel 26, the suction piston 28 is elastically connected with the inner wall of the air inlet channel 26 through the two springs 29, the push rod 210 is fixedly connected with the push rod 210 on one side wall of the air suction piston 28 far away from the outside, and the push rod 210 extends to the inside of the cavity 21, and the second pushing ball 211 is fixedly connected with the air.

It is worth mentioning that, one side that intake passage 26 is close to the external world is equipped with check valve 212, check valve 212 makes external air flow to the intake passage inside only, the air receiver 27 is through the exhaust passage 213 and the gasbag 13 inner space intercommunication of below, exhaust passage 213 inside is equipped with control valve 214, because the centrifuging tube is often faster when rotating centrifugation, therefore can make first push ball 25 stay in place through inertial force, and then make first push ball 25 can reciprocal striking second push ball 211, make second push ball 211 drive push rod 210 and suction piston 28 carry out reciprocal horizontal displacement (in the in-process of body 1 pivoted, centrifugal force can't make spring 29 extend, only when push rod 210 is to the right side displacement, make spring 29 extend, and when first push ball 25 no longer strikes second push ball 211, and the suction piston 28 can get back to the initial position under the elasticity effect of spring 29), and make the reciprocal outside air of suction piston 28, store it in intake chamber 27, need draw the monocyte layer after the centrifugation is accomplished, need draw control valve 214, can's high pressure air receiver 13 is gone out to the high-speed, and the high-speed air-exhausting valve 13 is arranged in the air receiver 13, and the high-speed air-exhausting valve 13 is opened, in addition, the high-speed air-exhausting valve 13 is convenient for the work is accomplished, the air receiver 13 is opened, and the air receiver is more greatly-inflated down.

The utility model can explain its functional principle by the following modes of operation:

when the tube body 1 is used, raw material liquid is placed into the tube body 1, the sealing sleeve 11 is placed above the tube body 1, at the moment, the sealing piston 14 is sealed and slides on the inner wall of the tube body 1, the sealing sleeve 11 and the tube body 1 are fixed through the fixing sleeve 111, the tube body 1 is placed into the centrifugal machine to carry out centrifugal operation, in the centrifugal process, the tube body 1 rotates, the first pushing ball 25 is enabled to impact the second pushing ball 211 in a reciprocating manner through inertia force, the suction piston 28 is enabled to carry out reciprocating horizontal displacement, outside air is sucked out, the outside air is discharged into the air storage chamber 27, after the centrifugal operation is completed, the tube body 1 is taken down, then the control valve 214 is opened, high-pressure air in the air storage chamber 27 enters the air bag 13, the air bag 13 is inflated, the sealing piston 14 is pushed to move downwards in the tube body 1, the monocyte layer is rapidly discharged from the lower side, the monocyte layer extraction efficiency is greatly improved, and after the extraction is completed, the exhaust valve is opened, and the high-pressure air is discharged from the air bag 13.

The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.

Claims (6)

1. Monocyte high efficiency draws centrifuging tube, its characterized in that includes:

the pipe body (1), the top of pipe body (1) is equipped with seal cover (11), empty slot (12) have been seted up to the lower wall of seal cover (11), be equipped with gasbag (13) in empty slot (12), the below fixedly connected with seal piston (14) of gasbag (13), seal piston (14) seal sliding fit is in the inner wall of pipe body (1);

the pushing assembly (2), the pushing assembly (2) comprises a cavity (21), a rotating shaft (22), a lantern ring (23), a connecting rod (24), a first pushing ball (25), an air inlet channel (26), an air storage chamber (27), a suction piston (28), a spring (29), a push rod (210) and a second pushing ball (211), the cavity (21) is formed in the sealing sleeve (11), the rotating shaft (22) is connected with the rotating shaft (21) in a rotating manner, the lantern ring (23) is sleeved on the rotating shaft (22), the connecting rod (24) is fixedly connected with the lantern ring (23), one side, far away from the lantern ring (23), of the connecting rod (24) is fixedly connected with the first pushing ball (25), the air inlet channel (26) is formed in the sealing sleeve (11), the air storage chamber (27) is formed below the air inlet channel (26) in the sealing sleeve (11), one side of the air inlet channel (26) is communicated with the outside, the other side of the air inlet channel (26) is communicated with the air storage chamber (27), the suction piston (28) is in a sealing sliding fit in the air inlet channel (26), the suction piston (28) is fixedly connected with the outer side wall (210) of the air inlet channel (29) through two springs (29), the push rod (210) extends to the inside of the cavity (21) and is fixedly connected with a second push ball (211).

2. The efficient monocyte extraction centrifuge tube of claim 1 wherein:

the lower part of the sealing sleeve (11) is fixedly connected with a fixing sleeve (111), and the fixing sleeve (111) can be fixed on the side wall of the pipe body (1).

3. The efficient monocyte extraction centrifuge tube of claim 1 wherein:

one side of the air inlet channel (26) close to the outside is provided with a one-way valve (212), and the one-way valve (212) enables the outside air to flow into the air inlet channel only.

4. The efficient monocyte extraction centrifuge tube of claim 1 wherein:

the air storage chamber (27) is communicated with the inner space of the air bag (13) through an exhaust passage (213) below.

5. The efficient monocyte extraction centrifuge tube of claim 4 wherein:

a control valve (214) is arranged in the exhaust passage (213).

6. The efficient monocyte extraction centrifuge tube of claim 1 wherein:

an exhaust valve is arranged on the air bag (13).