CN219546960U - Cell culture device for cell hypoxia environment - Google Patents

Abstract

The utility model relates to the technical field of cell culture devices and discloses a cell culture device for a cell anoxic environment, which comprises a culture dish, wherein a flowmeter and a gas cylinder are arranged on the left side of the culture dish, the upper side wall of the gas cylinder is fixedly communicated with a gas outlet pipe, a pressure reducing valve is communicated with the outside of the gas outlet pipe, the gas outlet end of the pressure reducing valve is fixedly communicated with the gas inlet end of the flowmeter, the culture dish comprises a base, an upper cover and an interlayer, the interlayer is positioned in the base, the upper cover is connected with the base through a connecting mechanism, connecting pipes are fixedly communicated on the left side and the right side of the base, and a blocking box is fixedly communicated on the side wall of the connecting pipe far away from the base. According to the utility model, after the mixed gas injection is finished, the culture dish can be immediately brought into a sealing state, so that the problem that the oxygen content in the culture dish is increased due to the fact that external air enters the culture dish is avoided, the culture dish can be conveniently and rapidly disassembled and assembled, and the convenience of the operation personnel is improved.

Description

Cell culture device for cell hypoxia environment

Technical Field

The utility model relates to the technical field of cell culture devices, in particular to a cell culture device for a cell anoxic environment.

Background

Cell culture refers to a method for simulating in-vivo environment (sterile, proper temperature, pH value, certain nutrition condition and the like) in vitro, so that the cell culture can survive, grow, reproduce and maintain main structures and functions, and a large number of cells can be obtained through the cell culture, and the signal transduction of the cells, the anabolism of the cells, the growth proliferation of the cells and the like can be studied.

When the conventional cell culture apparatus is used, in order to culture cells in an anoxic environment, it is generally necessary to inject a mixed gas into the culture apparatus, and after the mixed gas is injected, it is necessary to seal the culture dish at the very right end, and before the sealing, a part of air is easily introduced into the culture apparatus, so that the oxygen content in the culture apparatus is increased.

Disclosure of Invention

The utility model aims to solve the problems that when a cell culture device in the prior art is used, mixed gas is usually required to be injected into the culture device in order to culture cells in an anoxic environment, a culture dish is required to be blocked at the end immediately after the mixed gas is injected, and a part of air is easy to enter the culture device before the blocking, so that the oxygen content in the culture device is increased.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

the utility model provides a cell culture apparatus for cell anoxic environment, includes the culture dish, flowmeter and gas cylinder have been placed in the left side of culture dish, the fixed intercommunication of upper sidewall of gas cylinder has the outlet duct, the outer intercommunication of outlet duct has the relief pressure valve, the fixed intercommunication of the inlet end of the end and flowmeter that gives vent to anger of relief pressure valve, the culture dish includes base, upper cover and interlayer, the interlayer is located the base, upper cover and base are connected through coupling mechanism, the equal fixed intercommunication in left and right sides of base has the connecting pipe, the fixed intercommunication of lateral wall that the base was kept away from to the connecting pipe has the baffle box, the inner wall fixedly connected with retaining ring of baffle box, the lateral wall of retaining ring articulates there is the baffle, fixedly connected with spring between baffle and the retaining ring is located the left side the fixed intercommunication of lateral wall of baffle box has the gas injection pipe, is located the right the fixed intercommunication of lateral wall of baffle box has the blast pipe, the end and the gas injection pipe intercommunication of flowmeter.

Preferably, the same communicating pipe is inserted between the air outlet end of the flowmeter and the air injection pipe, and a plurality of non-return rings are fixedly sleeved on the outer wall of the communicating pipe.

Preferably, the connecting mechanism comprises a connecting ring fixedly sleeved on the outer walls of the base and the upper cover, the same fixing band is sleeved outside the connecting ring, the cross section of the fixing band is of a U-shaped structure, the side walls at the two ends of the fixing band are fixedly connected with connecting blocks, the side walls of the two connecting blocks are provided with connecting grooves, the same threaded pin is inserted into the connecting grooves, and nuts are sleeved outside the threaded pins.

Preferably, the lower side wall of the upper cover is fixedly connected with a sealing ring, and the upper side wall of the base is provided with a sealing groove matched with the sealing ring.

Preferably, the side wall of the blocking plate is fixedly connected with a rubber pad.

Preferably, the inner wall fixedly connected with of base places the circle, the interlayer is located places the circle, the lower lateral wall fixedly connected with of interlayer two fixture blocks, place the last lateral wall of circle and offered two draw-in grooves with fixture block mutual matching.

Compared with the prior art, the utility model provides a cell culture device for a cell anoxic environment, which has the following beneficial effects:

1. this a cell culture apparatus for cell anoxic environment through culture dish, flowmeter, gas cylinder, outlet duct, relief pressure valve, base, upper cover, interlayer, connecting pipe, block case, retaining ring, barrier plate, gas filling pipe, blast pipe that set up, can make the culture dish get into sealing state immediately after the mixed gas injection finishes to avoid outside air to get into the culture dish and can cause the problem that the oxygen content risees in the culture dish.

2. This a cell culture apparatus for cell anoxic environment through go-between, fixed band, connecting block, threaded pin, the nut that sets up, dismouting culture dish that can be convenient quick has improved the convenience of operating personnel work.

The device has the advantages that the device has no part which is the same as or can be realized by adopting the prior art, and the culture dish can be immediately in a sealed state after the mixed gas injection is finished, so that the problem that the oxygen content in the culture dish is increased due to the fact that external air enters the culture dish is avoided, the culture dish can be disassembled and assembled conveniently and rapidly, and the convenience of the work of operators is improved.

Drawings

FIG. 1 is a schematic diagram of a cell culture apparatus for use in a hypoxic environment in accordance with the utility model;

FIG. 2 is an enlarged schematic view of portion A of FIG. 1;

FIG. 3 is a schematic diagram showing the internal structure of a barrier tank in a cell culture apparatus for a cell anoxic environment according to the present utility model;

FIG. 4 is a schematic diagram showing the connection relationship of the fixing bands in a cell culture apparatus for a cell hypoxic environment according to the utility model;

fig. 5 is an enlarged schematic view of the portion B in fig. 4.

In the figure: 1. a culture dish; 2. a flow meter; 3. a gas cylinder; 4. an air outlet pipe; 5. a pressure reducing valve; 6. a base; 7. an upper cover; 8. an interlayer; 9. a connecting pipe; 10. a blocking box; 11. a retainer ring; 12. a blocking plate; 13. an air injection pipe; 14. an exhaust pipe; 15. a communicating pipe; 16. a non-return ring; 17. a connecting ring; 18. a fixing belt; 19. a connecting block; 20. a threaded pin; 21. a nut; 22. a seal ring; 23. a rubber pad; 24. placing a ring; 25. and (5) clamping blocks.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.

Referring to fig. 1-5, a cell culture device for cell anoxic environment, including culture dish 1, flowmeter 2 and gas cylinder 3 have been placed in the left side of culture dish 1, the upper side wall of gas cylinder 3 fixedly communicates there is outlet duct 4, the outer intercommunication of outlet duct 4 has relief pressure valve 5, the end that gives vent to anger of relief pressure valve 5 and the inlet end of flowmeter 2 fixedly communicate, culture dish 1 includes base 6, upper cover 7 and interlayer 8, interlayer 8 is located base 6, upper cover 7 and base 6 are connected through coupling mechanism, the left and right sides of base 6 all fixedly communicates there is connecting pipe 9, the lateral wall that connecting pipe 9 kept away from base 6 fixedly communicates there is the baffle box 10, the inner wall fixedly connected with retaining ring 11 of baffle box 10, the lateral wall hinge of retaining ring 11 has barrier plate 12, fixedly connected with spring between barrier plate 12 and the retaining ring 11, the lateral wall that is located left side baffle box 10 fixedly communicates there is gas injection pipe 13, the lateral wall that is located right side baffle box 10 fixedly communicates there is blast pipe 14, the end that gives vent to anger of flowmeter 2 communicates with gas injection pipe 13.

The same communicating pipe 15 is inserted between the air outlet end of the flowmeter 2 and the air injection pipe 13, a plurality of non-return rings 16 are fixedly sleeved on the outer wall of the communicating pipe 15, and the air outlet end of the flowmeter 2 and the air injection pipe 12 can be communicated.

The coupling mechanism establishes the go-between 17 at base 6 and upper cover 7 outer wall including fixed cover, and the go-between 17 overcoat is equipped with same fixed band 18, and the cross section of fixed band 18 is U type structure, and the equal fixedly connected with connecting block 19 of both ends lateral wall of fixed band 18, the spread groove has all been seted up to the lateral wall of two connecting blocks 19, has pegged graft in two spread grooves and has same root screw thread round pin 20, and screw thread round pin 20 external screw thread cover is equipped with nut 21, dismouting culture dish that can be convenient quick has improved the convenience of operating personnel work.

The sealing ring 22 is fixedly connected to the lower side wall of the upper cover 7, and the sealing groove matched with the sealing ring 22 is formed in the upper side wall of the base 6, so that the stability of up-and-down movement of the upper cover 7 and the base 6 is improved.

The side wall of the blocking plate 12 is fixedly connected with a rubber pad 23, so that the sealing performance of the blocking plate 12 is improved.

The inner wall fixedly connected with of base 6 places circle 24, and interlayer 8 is located places circle 24, and the lower lateral wall fixedly connected with of interlayer 8 two fixture blocks 25, place the draw-in groove that two and fixture block 25 mutually match has been seted up to the last lateral wall of circle 24, has improved the stability that interlayer 8 placed.

In the utility model, when the gas in the gas cylinder 3 can be conveyed to the culture dish 1 through the pressure reducing valve 5, the gas in the gas cylinder 3 enters the gas injection pipe 13 through the flowmeter 2, the gas pressure at the left side of the baffle plate 12 can be increased, the redundant gas can push the baffle plate 12, the gas can enter the culture dish 1 through the connecting pipe 9, the oxygen in the culture dish 1 can be discharged through the gas injection pipe 13 and the exhaust pipe 14, and after the gas injection is finished, the baffle plate 12 can block the check ring 11 under the tensile force of the spring, so that the culture dish 1 is sealed.

Claims (6)

1. The utility model provides a cell culture apparatus for cell hypoxia environment, includes culture dish (1), its characterized in that, flowmeter (2) and gas cylinder (3) have been placed in the left side of culture dish (1), the fixed intercommunication of upper side wall of gas cylinder (3) has outlet duct (4), outlet duct (4) external communication has relief valve (5), the fixed intercommunication of the end of giving vent to anger of relief valve (5) and the inlet end of flowmeter (2), culture dish (1) include base (6), upper cover (7) and interlayer (8), interlayer (8) are located base (6), upper cover (7) and base (6) are connected through coupling mechanism, the left and right sides of base (6) all are fixed to be linked together and are had connecting pipe (9), the fixed intercommunication of lateral wall that base (6) was kept away from to connecting pipe (9) has barrier box (10), the inner wall fixedly connected with retaining ring (11) of barrier box (10), the lateral wall hinge of retaining ring (11) has barrier plate (12), be connected with between barrier plate (12) and retaining plate (11) and barrier plate (14) are located the fixed side wall (13) of gas injection box (13), the air outlet end of the flowmeter (2) is communicated with the air injection pipe (13).

2. Cell culture device for cell anoxic environment according to claim 1, characterized in that the same communicating tube (15) is inserted between the air outlet end of the flowmeter (2) and the air injection tube (13), and the outer wall of the communicating tube (15) is fixedly sleeved with a plurality of non-return rings (16).

3. The cell culture device for the cell anoxic environment according to claim 1, wherein the connecting mechanism comprises a connecting ring (17) fixedly sleeved on the outer walls of the base (6) and the upper cover (7), the same fixing belt (18) is sleeved outside the connecting ring (17), the cross section of the fixing belt (18) is of a U-shaped structure, connecting blocks (19) are fixedly connected to the side walls of the two ends of the fixing belt (18), connecting grooves are formed in the side walls of the two connecting blocks (19), the same threaded pin (20) is inserted into the two connecting grooves, and nuts (21) are sleeved outside the threaded pins (20).

4. The cell culture apparatus for a cell anoxic environment according to claim 1, wherein the lower side wall of the upper cover (7) is fixedly connected with a sealing ring (22), and the upper side wall of the base (6) is provided with a sealing groove matched with the sealing ring (22).

5. Cell culture device for the hypoxic environment of cells according to claim 1, characterized in that the side wall of the barrier plate (12) is fixedly connected with a rubber pad (23).

6. The cell culture device for the cell anoxic environment according to claim 1, wherein the inner wall of the base (6) is fixedly connected with a placement ring (24), the interlayer (8) is positioned on the placement ring (24), the lower side wall of the interlayer (8) is fixedly connected with two clamping blocks (25), and the upper side wall of the placement ring (24) is provided with two clamping grooves matched with the clamping blocks (25).