CN219489978U - Cell separation sieving mechanism - Google Patents

Abstract

The utility model discloses a cell separation screening device which comprises a machine shell, wherein a feeding hopper is arranged above the machine shell, a guide hopper is fixedly connected to the top wall of an inner cavity of the machine shell, a separation barrel is arranged below the guide hopper, a rotary joint is arranged between the guide hopper and the separation barrel, the guide hopper is connected with the separation barrel through the rotary joint, a separation assembly is arranged below the separation barrel, a guide table is symmetrically arranged below the separation assembly, the guide table is obliquely arranged, the higher inclined end of the guide table is fixedly connected with the inner side wall of the machine shell, a screening frame is arranged below the guide table, screening assemblies are symmetrically arranged on two sides of the screening frame, and a discharge pipe is communicated with the bottom wall of the machine shell.

Description

Cell separation sieving mechanism

Technical Field

The utility model relates to a separation and screening device, in particular to a cell separation and screening device, and belongs to the technical field of CAR-T cells.

Background

T lymphocyte is derived from bone marrow multipotent stem cell, and in embryo stage and primary stage of human body, some multipotent stem cell or pre-T cell in bone marrow is transferred into thymus, and is differentiated and matured under the induction of thymus hormone to obtain T cell with immunological activity, and the matured T cell is distributed to thymus dependent region of peripheral immunological organ via blood flow, and can be recirculated via lymphatic vessel, peripheral blood and tissue fluid, so that it can implement the functions of cell immunity and immunoregulation, and the recirculation of T cell is favorable for extensive contact with antigen material into body, raising immunological response, long-period retaining immunological memory, and the cell membrane of T cell has several different marks, mainly surface antigen and surface receptor, these surface marks are giant protein molecules bound on cell membrane.

In the technical field of CAR-T cells, cell separation and screening are required, and therefore a separation and screening device is required, and a conventional separation and screening device only has a single separation and screening capability, and has some disadvantages, such as a single screening mode, so that the screening capability is poor, the effect is not ideal, and the subsequent operation can be influenced.

Disclosure of Invention

The present utility model has been made to solve the above-mentioned problems, and an object of the present utility model is to provide a cell separation and screening apparatus which has a good screening ability and an ideal effect because of various screening methods, and thus does not affect the subsequent operations.

The utility model realizes the purpose through the following technical scheme, the device comprises a shell, a feeding hopper is arranged above the shell, a guide hopper is fixedly connected to the top wall of an inner cavity of the shell, a separation barrel is arranged below the guide hopper, a rotary joint is arranged between the guide hopper and the separation barrel, the guide hopper is connected with the separation barrel through the rotary joint, a separation assembly is arranged below the separation barrel, a guide table is symmetrically arranged below the separation assembly, the guide table is obliquely arranged, the obliquely higher end of the guide table is fixedly connected with the inner side wall of the shell, a screening frame is arranged below the guide table, screening assemblies are symmetrically arranged on two sides of the screening frame, and the bottom wall of the shell is communicated with a discharge pipe.

Preferably, the bottom of the feeding hopper penetrates through the top wall of the shell and is communicated with the guide hopper, a protective cover is rotatably connected to one side of the top end of the feeding hopper through a hinge shaft, and an opening and closing handle is fixedly connected to the protective cover.

Preferably, the separation assembly comprises a driving motor fixedly connected with the side wall of the casing, a first transmission shaft is fixedly connected to the output end of the driving motor, the first transmission shaft is far away from one end of the driving motor, penetrates through the side wall of the casing and extends to the inner cavity of the casing, a first bevel gear is arranged at the extending end of the first transmission shaft, a second bevel gear is connected to the first bevel gear in a meshed manner, and a second transmission shaft is arranged on the second bevel gear and fixedly connected with the center of the bottom wall of the separation barrel.

Preferably, the inner cavity of the screening frame is provided with a first microporous filter screen, the bottom wall of the screening frame is provided with a second microporous filter screen, and the mesh diameter of the first microporous filter screen is larger than that of the second microporous filter screen.

Preferably, sliding blocks are fixedly connected to the tops of two side walls of the screening frame, a movable groove is formed in the bottom wall of the inner cavity of the material guide table, and the screening frame is horizontally and slidably connected with the movable groove through the sliding blocks.

Preferably, the screening assembly comprises electric telescopic rods which are symmetrically and fixedly connected with two side walls of the machine shell, wherein the end parts of the electric telescopic rods penetrate through the side walls of the machine shell and extend to the inner cavity of the machine shell, and the output ends of the electric telescopic rods are fixedly connected with the side walls of the screening frame.

The beneficial effects of the utility model are as follows: can be with the cell leading-in casing through setting up into the hopper, get into guide hopper and finally leading-in to the separation bucket in, can protect into the hopper when not leading the material through setting up the protective cover, prevent that the casing inner chamber from getting into debris through the hopper, can drive the separation bucket through setting up the separation subassembly and carry out cell separation, driving motor can drive first transmission shaft and first bevel gear and rotate, first bevel gear drives second bevel gear and second transmission shaft and rotates, thereby drive the separation bucket and rotate, and utilize the centrifugal force that produces in the separation bucket to separate the cell, can carry out primary screening to the cell after the separation through setting up the guide table in the screening frame, can carry out the rescreening to the cell through setting up first micropore filter screen, can improve the screening effect through setting up the screening subassembly, electric telescopic handle can drive the screening frame, the screening frame can carry out reciprocal concussion through the sliding block in the movable tank, thereby still can improve the ability of first micropore filter screen and second micropore filter screen under the prerequisite that avoids first micropore filter screen and second micropore filter screen to block up.

Drawings

FIG. 1 is a front view of a cell separation and screening apparatus according to the present utility model;

FIG. 2 is a schematic diagram showing the structure of a cell separation and screening device according to the present utility model;

FIG. 3 is a schematic diagram showing the structure of a hopper of a cell separation and screening apparatus according to the present utility model;

FIG. 4 is a schematic diagram showing the structure of a screening assembly of a cell separation screening apparatus according to the present utility model;

in the figure: 1. a housing; 2. feeding into a hopper; 3. a guide hopper; 4. a separation barrel; 5. a rotary joint; 6. a driving motor; 7. a first drive shaft; 8. a second drive shaft; 9. a material guiding table; 10. a screening frame; 11. a first microporous screen; 12. a second microporous filter screen; 13. a sliding block; 14. a movable groove; 15. an electric telescopic rod; 16. and a discharge pipe.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1-4, a cell separation screening device comprises a casing 1, a feeding hopper 2 is arranged above the casing 1, a guide hopper 3 is fixedly connected to the top wall of an inner cavity of the casing 1, a separation barrel 4 is arranged below the guide hopper 3, a rotary joint 5 is arranged between the guide hopper 3 and the separation barrel 4, the guide hopper 3 and the separation barrel 4 are connected through the rotary joint 5, a separation assembly is arranged below the separation barrel 4, a guide table 9 is symmetrically arranged below the separation assembly, the guide table 9 is obliquely arranged, the obliquely higher end of the guide table 9 is fixedly connected with the inner side wall of the casing 1, a screening frame 10 is arranged below the guide table 9, screening assemblies are symmetrically arranged on two sides of the screening frame 10, and a discharge pipe 16 is communicated with the bottom wall of the casing 1.

As a preferable technical scheme of the utility model, the bottom end of the feeding hopper 2 penetrates through the top wall of the shell 1 and is communicated with the guide hopper 3, one side of the top end of the feeding hopper 2 is rotatably connected with a protective cover through a hinge shaft, and an opening and closing handle is fixedly connected on the protective cover.

As a preferred technical scheme of the utility model, the separation assembly comprises a driving motor 6 fixedly connected to the side wall of the casing 1, a first transmission shaft 7 is fixedly connected to the output end of the driving motor 6, one end of the first transmission shaft 7, which is far away from the driving motor 6, penetrates through the side wall of the casing 1 and extends to the inner cavity of the casing 1, a first bevel gear is arranged at the extending end of the first transmission shaft 7, a second bevel gear is connected to the first bevel gear in a meshed manner, a second transmission shaft 8 is arranged on the second bevel gear, and the second transmission shaft 8 is fixedly connected with the center of the bottom wall of the separation barrel 4.

As a preferred embodiment of the present utility model, a first micro-pore filter screen 11 is disposed in the inner cavity of the screening frame 10, a second micro-pore filter screen 12 is disposed on the bottom wall of the screening frame 10, and the mesh diameter of the first micro-pore filter screen 11 is larger than the diameter of the second micro-pore filter screen 12.

As a preferable technical scheme of the utility model, sliding blocks 13 are fixedly connected to the tops of two side walls of the screening frame 10, a movable groove 14 is formed in the bottom wall of the inner cavity of the material guiding table 9, and the screening frame 10 is horizontally and slidably connected with the movable groove 14 through the sliding blocks 13.

As a preferred technical scheme of the utility model, the screening assembly comprises electric telescopic rods 15 which are symmetrically and fixedly connected to two side walls of the casing 1, wherein the end parts of the electric telescopic rods 15 penetrate through the side walls of the casing 1 and extend to the inner cavity of the casing 1, and the output ends of the electric telescopic rods 15 are fixedly connected with the side walls of the screening frame 10.

According to the utility model, cells can be led into the shell 1 through the arrangement of the feeding hopper 2, enter the guide hopper 3 and finally be led into the separation barrel 4, the feeding hopper 2 can be protected when no materials are led through the arrangement of the protective cover, the inner cavity of the shell 1 is prevented from entering sundries through the feeding hopper 2, the separation barrel 4 can be driven to separate the cells through the arrangement of the separation assembly, the driving motor 6 can drive the first transmission shaft 7 and the first bevel gear to rotate, the first bevel gear drives the second bevel gear and the second transmission shaft 8 to rotate, the separation barrel 4 is driven to rotate, the cells are separated through centrifugal force generated in the separation barrel 4, the separated cells can be led into the screening frame 10 through the arrangement of the guide table 9, the cells can be screened for the first time through the arrangement of the first microporous filter screen 11, the cells can be screened again through the arrangement of the second microporous filter screen 12, the screening effect can be improved through the arrangement of the screening assembly, the electric telescopic rod 15 can regularly push the screening frame 10, and the reciprocating vibration of the screening frame 10 in the movable groove 14 can be avoided through the sliding block 13, and the screening capacity of the first microporous filter screen 11 and the second microporous filter screen 12 can be improved on the premise that the second microporous filter screen 11 is blocked.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.

Claims (6)

1. Cell separation sieving mechanism, including casing (1), its characterized in that: the utility model discloses a screening machine, including casing (1), inner chamber roof fixedly connected with guide hopper (3), the below of guide hopper (3) is equipped with separating drum (4), guide hopper (3) with be equipped with rotary joint (5) between separating drum (4) just guide hopper (3) with pass through between separating drum (4) rotary joint (5) are connected, the below of separating drum (4) is equipped with separating subassembly, separating subassembly's below symmetry is equipped with guide platform (9), guide platform (9) set up just for the slope guide platform (9) slope higher end with the inside wall fixed connection of casing (1), the below of guide platform (9) is equipped with screening frame (10), the bilateral symmetry of screening frame (10) is equipped with screening subassembly, the diapire intercommunication of casing (1) has row material pipe (16).

2. The cell separation and screening device according to claim 1, wherein: the bottom of the feeding hopper (2) penetrates through the top wall of the shell (1) and is communicated with the guide hopper (3), a protective cover is rotatably connected to one side of the top of the feeding hopper (2) through a hinge shaft, and an opening and closing handle is fixedly connected to the protective cover.

3. The cell separation and screening device according to claim 1, wherein: the separation assembly comprises a driving motor (6) fixedly connected with the side wall of the casing (1), a first transmission shaft (7) is fixedly connected to the output end of the driving motor (6) and is far away from the first transmission shaft (7), one end of the driving motor (6) penetrates through the side wall of the casing (1) and extends to the inner cavity of the casing (1), a first bevel gear is arranged at the extending end of the first transmission shaft (7), a second bevel gear is connected to the first bevel gear in a meshed mode, and a second transmission shaft (8) is arranged on the second bevel gear and fixedly connected with the center of the bottom wall of the separation barrel (4).

4. The cell separation and screening device according to claim 1, wherein: the inner cavity of the screening frame (10) is provided with a first microporous filter screen (11), the bottom wall of the screening frame (10) is provided with a second microporous filter screen (12), and the mesh diameter of the first microporous filter screen (11) is larger than the diameter of the second microporous filter screen (12).

5. The cell separation and screening device according to claim 1, wherein: the screening device is characterized in that sliding blocks (13) are fixedly connected to the tops of two side walls of the screening frame (10), a movable groove (14) is formed in the bottom wall of an inner cavity of the material guide table (9), and the screening frame (10) is horizontally and slidably connected with the movable groove (14) through the sliding blocks (13).

6. The cell separation and screening device according to claim 1, wherein: the screening assembly comprises electric telescopic rods (15) which are symmetrically and fixedly connected with two side walls of the machine shell (1), wherein the end parts of the electric telescopic rods (15) penetrate through the side walls of the machine shell (1) and extend to the inner cavity of the machine shell (1), and the output ends of the electric telescopic rods (15) are fixedly connected with the side walls of the screening frame (10).