CN219434489U - Freezing embedding device - Google Patents

Abstract

The utility model discloses a freezing embedding device, and belongs to the technical field of tissue freezing embedding. The freezing embedding device comprises a lower box assembly and an upper box assembly, wherein the lower box assembly comprises a heat conduction lower box, a first containing cavity for containing a refrigerant is formed in the heat conduction lower box, a first containing structure is formed on the top surface of the heat conduction lower box, the upper box assembly is buckled on the lower box assembly, the upper box assembly comprises a heat conduction upper box, a second containing cavity for containing the refrigerant is formed in the heat conduction upper box, a second containing structure is formed on the bottom surface of the heat conduction upper box, and the second containing structure and the first containing structure are matched to form a third containing cavity for containing the metal embedding box. The freezing embedding device not only can enable the biological tissues in the metal embedding box to be cooled down rapidly, thereby reducing the generation amount of ice crystals, but also is easy to carry by users so as to transfer the biological tissues stably.

Description

Freezing embedding device

Technical Field

The utility model relates to the technical field of tissue freezing embedding, in particular to a freezing embedding device.

Background

Frozen section (frozen section) is a method of slicing biological tissue after it is rapidly cooled to a certain hardness under low temperature conditions. The frozen section is faster and simpler than paraffin section in the process of making, so that the frozen section is applied to rapid pathological diagnosis in operation. Although the frozen section is required to be embedded with tissues, the embedding and freezing process is easy to cause water crystallization of the tissues to influence the morphological structure of cells and the positioning of antigen substances, and the section and dyeing effect are not affected by overlarge tissues or uneven freezing of the tissues, so that the frozen section has no wide application range of paraffin sections.

The existing tissue freezing and embedding methods mainly comprise three methods of liquid nitrogen direct freezing, liquid nitrogen isopentane freezing and OCT plastic embedding box embedding, and compared with the liquid nitrogen direct freezing and the liquid nitrogen isopentane freezing, the OCT plastic embedding box embedding can effectively prevent the morphological damage of the periphery of biological tissues, and meanwhile, the temperature of OCT and the temperature of the biological tissues can be kept consistent to prevent OCT and tissue separation, so that the smooth implementation of tissue sections is facilitated.

However, the embedding boxes used in the prior OCT plastic embedding boxes have slow heat conduction of plastics, and the formation of ice crystals is positively related to the cooling speed, so that a large amount of ice crystals are formed in the tissues in the tissue embedding process, the existence of the ice crystals can destroy the morphology of the tissues, and the subsequent pathological reading and molecular biological analysis of frozen tissues can be influenced. In addition, existing cassette are not easily portable for transfer.

Therefore, how to provide a freezing and embedding device which can quickly cool tissues and is easy to carry is a technical problem which needs to be solved at present.

Disclosure of Invention

The utility model aims to provide a freezing embedding device which can quickly cool tissues and is easy to carry.

To achieve the purpose, the utility model adopts the following technical scheme:

a freeze-embedding device comprising: the lower box assembly comprises a heat-conducting lower box, a first containing cavity for containing refrigerant is formed in the heat-conducting lower box, and a first containing structure is formed on the top surface of the heat-conducting lower box; go up the box subassembly, it is in to go up the box subassembly cartridge is in on the lower box subassembly, go up the box subassembly and include the heat conduction and go up the box, be formed with in the heat conduction and hold the second of refrigerant holds the chamber, be formed with the second on the bottom surface of heat conduction and hold the structure, the second hold the structure with first hold the structure cooperation and form the third that is used for holding metal embedding box and holds the chamber.

Preferably, the heat conducting lower box comprises a metal lower box and a metal lower cover, wherein a first opening is formed at the top of the metal lower box, the metal lower cover is buckled on the first opening, and the first accommodating structure is formed on the top surface of the metal lower cover; and/or, the heat conduction upper box comprises a metal upper box, a second opening is formed at the top of the metal upper box, the second opening can be in a blocking state, and the second accommodating structure is formed on the bottom surface of the metal upper box.

Preferably, the lower box assembly further comprises a heat-insulating lower box, a third opening is formed at the top of the heat-insulating lower box, and the heat-conducting lower box is arranged in the heat-insulating lower box through the third opening; the upper box assembly further comprises a heat-insulating upper box, a fourth opening is formed in the bottom of the heat-insulating upper box, the heat-conducting upper box is arranged in the heat-insulating upper box, and the second accommodating structure protrudes out of the heat-insulating upper box through the fourth opening.

Preferably, the heat-insulating upper box comprises a heat-insulating ring sleeve and a heat-insulating box cover, wherein the heat-insulating ring sleeve is arranged outside the metal upper box in a sleeved mode, and the heat-insulating box cover is detachably connected in the second opening so as to seal the second opening.

Preferably, the heat preservation box cover is convexly provided with a plug-in part, and the plug-in part is inserted into the top opening of the heat preservation ring sleeve.

Preferably, a first step surface is formed at the inner wall surface of the heat-insulating ring sleeve, a second step surface is formed at the outer wall surface of the metal upper box, and the second step surface is abutted to the first step surface.

Preferably, one of the heat-insulating lower box and the heat-insulating upper box is provided with a buckling groove along the circumferential direction, and the other is provided with a buckling protrusion along the circumferential direction, and the buckling protrusion is inserted into the buckling groove.

Preferably, the metal lower cover is provided with a first annular side wall buckled outside the metal lower box, an annular groove is formed at the opening of the heat-insulation lower box, and the first annular side wall is arranged in the annular groove.

Preferably, one of the first accommodating structure and the second accommodating structure is an accommodating groove, the other is a protrusion, the protrusion is arranged on the upper portion of the accommodating groove and seals the notch of the accommodating groove, and the protruding outer wall surface and the inner wall surface of the accommodating groove enclose to form the third accommodating cavity.

Preferably, the number of the first accommodating structures and the number of the second accommodating structures are multiple, and the first accommodating structures and the second accommodating structures are arranged in a one-to-one correspondence manner so as to form the third accommodating cavities.

The utility model has the beneficial effects that:

the utility model provides a freezing embedding device which comprises a lower box assembly and an upper box assembly, wherein the lower box assembly comprises a heat conduction lower box, a first containing cavity for containing refrigerant is formed in the heat conduction lower box, a first containing structure is formed on the top surface of the heat conduction lower box, the upper box assembly is buckled on the lower box assembly, the upper box assembly comprises a heat conduction upper box, a second containing cavity for containing refrigerant is formed in the heat conduction upper box, a second containing structure is formed on the bottom surface of the heat conduction upper box, and the second containing structure and the first containing structure are matched to form a third containing cavity for containing a metal embedding box. The freezing embedding device can enable the biological tissue in the metal embedding box to be rapidly cooled, so that the generation amount of ice crystals is reduced, and the freezing embedding device is easy to carry by a user, so that the biological tissue is stably transferred.

Drawings

FIG. 1 is a schematic view of a freezing and embedding device according to the present utility model;

FIG. 2 is an exploded view of the freeze-pack device provided by the present utility model;

fig. 3 is a cross-sectional view of a freeze-embedding device provided by the present utility model.

In the figure:

100. a lower box assembly;

101. a metal lower box; 102. a metal lower cover; 1021. a first receiving structure; 1022. a first annular sidewall; 103. preserving heat of the lower box; 1031. a buckling groove; 1032. a ring groove;

200. an upper box assembly;

201. a metal upper case; 2011. a second receiving structure; 202. a heat-insulating ring sleeve; 2021. a buckling bulge; 203. a thermal insulation box cover; 2031. a plug-in part;

300. a metal embedding box.

Detailed Description

The following description of the embodiments of the present utility model will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the utility model are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixed or removable, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

As shown in fig. 1 to 3, the present utility model provides a freezing and embedding device including a lower case assembly 100 and an upper case assembly 200, the upper case assembly 200 being fastened to the lower case assembly 100, and a metal embedding case 300 being interposed between the upper case assembly 200 and the lower case assembly 100 and being covered by the fastened upper case assembly 200 and lower case assembly 100.

Specifically, the lower box assembly 100 includes a heat-conducting lower box, which is a box-shaped structure made of heat-conducting materials, and the heat-conducting lower box can be a cube box, a round box or a box body with other shapes, wherein metal, alloy or other materials with good heat-conducting performance can be selected as the heat-conducting materials according to requirements. The heat conduction lower box is internally provided with a first accommodating cavity for accommodating the refrigerant. In some embodiments, the refrigerant selects dry ice, and the dry ice may be in the form of a block, powder or column, and the cross-sectional shape of the first receiving chamber may be set to be square, round or other shapes according to the need. A first receiving structure 1021 is formed on the top surface of the heat conductive lower case, and the first receiving structure 1021 is used to assist in forming a receiving space for receiving the metal embedding case 300.

The upper case assembly 200 includes a heat-conducting upper case, which is a box-shaped structure made of heat-conducting materials, and the heat-conducting upper case can be a cube case, a round case or a case body with other shapes, wherein the heat-conducting upper case can be made of metals, alloys or other materials with good heat-conducting properties according to requirements. In some embodiments, the thermally conductive upper and lower cassettes are preferably the same shape and material to facilitate assembly and to achieve heat transfer equalization. The thermally conductive upper case has a second accommodation chamber formed therein for accommodating a refrigerant, and in some embodiments, the refrigerant is dry ice, and the cross-sectional shape of the second accommodation chamber may be set to be square, circular, or other shapes as desired. A second accommodating structure 2011 is formed on the bottom surface of the upper heat conducting box, and the second accommodating structure 2011 and the first accommodating structure 1021 cooperate to form a third accommodating cavity, and the metal embedding box 300 is arranged in the third accommodating cavity and is used for embedding biological tissues.

According to the freezing embedding device provided by the utility model, the heat conduction upper box and the heat conduction lower box are made of materials with good heat conduction performance, the second accommodating cavity for accommodating the refrigerant is formed in the heat conduction upper box, the first accommodating cavity for accommodating the refrigerant is formed in the heat conduction lower box, and the metal embedding box 300 is arranged in the third accommodating cavity formed by the second accommodating structure 2011 of the heat conduction upper box and the first accommodating structure 1021 of the heat conduction lower box, so that the freezing embedding device has good heat conduction performance, the cold of the refrigerant can be quickly transferred into biological tissues arranged in the metal embedding box 300, the biological tissues can be quickly cooled, the effect of reducing the generation amount of ice crystals is achieved, the situation that excessive ice crystals damage the biological tissues is avoided, and the influence of the ice crystals on pathological reading and molecular biological analysis after the subsequent slicing of the biological tissues is avoided. In addition, the freezing embedding device adopts a split structure, is easy to assemble and convenient to carry and transfer, so that doctors or other workers can carry the freezing embedding device to the working environment, and long-time work is facilitated.

In some embodiments, with continued reference to fig. 2, the thermally conductive lower case includes a metal lower case 101 and a metal lower cover 102, the top of the metal lower case 101 forms a first opening, the metal lower cover 102 is snapped over the first opening, and a first receiving structure 1021 is formed on the top surface of the metal lower cover 102. The metal lower case 101 and the metal lower cover 102 have high heat transfer efficiency, and the separately arranged metal lower case 101 and metal lower cover 102 facilitate the taking and placing of the refrigerant. The inner wall surface of the metal lower box 101 and the inner wall surface of the metal lower cover 102 are surrounded to form a first accommodating cavity, and the dry ice in the first accommodating cavity can conduct cold to the metal lower cover 102 by utilizing the principle of rapid metal heat conduction, and ensures that the temperatures of the metal lower box 101 and the metal lower cover 102 are consistent with the dry ice temperature, namely, the temperatures are uniformly maintained at-80 ℃. The cooling capacity can be transferred to the metal embedding box 300 arranged at the first accommodation structure 1021 through the metal lower cover 102, so that the metal embedding box 300 can be cooled down quickly.

In some more specific embodiments, the metal lower cover 102 includes a top plate and a first annular sidewall 1022, the first annular sidewall 1022 protruding downward along a circumferential direction of the top plate, and the metal lower cover 102 is formed into a first receiving structure 1021 by punching, and in particular, the first receiving structure 1021 is a receiving groove. The metal lower case 101 includes a first bottom plate and a second annular side wall protruding upward in the circumferential direction of the first bottom plate. When the metal lower cover 102 is buckled in the metal lower box 101, the first annular side wall 1022 is located at the outer side of the second annular side wall, so that buckling and separation of the metal lower cover 102 and the metal lower box 101 are facilitated, the buckled metal lower cover 102 and metal lower box 101 are good in tightness, and dry ice located in the first containing cavity is not easy to overflow.

In order to avoid heat conduction between the inside and the outside of the heat conducting lower box and to protect operators from being frostbitten by the excessively low temperature of the dry ice, and to continue to refer to fig. 2, the lower box assembly 100 further includes a heat insulating lower box 103, and a third opening is formed at the top of the heat insulating lower box 103, and the heat conducting lower box is disposed in the heat insulating lower box 103 through the third opening. The insulation lower case 103 is made of a material having insulation properties, for example, the insulation lower case 103 may be a foam case made of foam. The shape of the inner cavity of the heat-insulating lower box 103 is matched with the shape of the heat-conducting lower box, so that the gap between the heat-conducting lower box and the heat-insulating lower box 103 is reduced, and the heat-insulating effect is further improved.

In some embodiments, a ring groove 1032 is formed at the opening of the thermal insulation lower case 103, and the first annular sidewall 1022 of the metal lower cover 102 is placed in the ring groove 1032. Thus, the matching tightness of the metal lower cover 102 and the heat-preserving lower box 103 can be improved, and the metal lower cover 102 and the heat-preserving lower box 103 are nested and clamped. Further, the width of the ring groove 1032 is greater than the thickness of the first annular sidewall 1022, so that an operation gap is formed between the inner wall surface of the ring groove 1032 and the outer wall surface of the first annular sidewall 1022, and the operation gap is provided to facilitate the user to apply force to the metal lower cover 102, so that the metal lower cover 102 can be smoothly opened from the metal lower case 101.

With continued reference to fig. 2, the thermally conductive upper case includes a metal upper case 201, a second opening is formed at the top of the metal upper case 201, the second opening can be opened or closed, and a second receiving structure 2011 is formed on the bottom surface of the metal upper case 201. The heat transfer efficiency of the metal upper case 201 is high, and the dry ice located in the metal upper case 201 can transfer heat into the metal embedding case 300 placed at the second receiving structure 2011 through the metal upper case 201, so that the temperatures of the metal upper case 201 and the metal embedding case 300 are maintained at-80 ℃. In some embodiments, the thermally conductive upper case further comprises a metal upper cover that snaps onto the metal upper case 201 to close off the second opening.

The metal upper case 201 includes a second bottom plate and a third annular side wall protruding upward around the circumference of the second bottom plate. In some embodiments, the second accommodating structure 2011 may be formed on the second bottom plate of the metal upper case 201 by punching the second bottom plate of the metal upper case 201, specifically, the second accommodating structure 2011 is a protrusion, the protrusion is disposed on the upper portion of the accommodating groove and seals the notch of the accommodating groove, and the outer wall surface of the protrusion and the inner wall surface of the accommodating groove enclose to form a third accommodating cavity. Of course, the positions of the protrusions and the receiving grooves may be interchanged, that is, the receiving grooves are formed in the metal upper case 201 and the protrusions are formed in the metal lower cover 102.

It should be noted that, the shape of the third accommodating cavity formed by the protrusion and the accommodating groove is the same as the shape and the same size of the metal embedding box 300, so that the metal embedding box 300 can be tightly placed in the third accommodating cavity, the gap between the inner wall surface of the third accommodating cavity and the outer wall surface of the metal embedding box 300 is reduced, the heat conduction efficiency is further improved, and the temperature of the metal embedding box 300 is quickly reduced to be consistent with the temperature of dry ice.

Further, the number of the first accommodation structures 1021 and the number of the second accommodation structures 2011 are plural, and the plurality of first accommodation structures 1021 and the plurality of second accommodation structures 2011 are disposed in one-to-one correspondence to form a plurality of third accommodation cavities. In some embodiments, the plurality of first receiving structures 1021 and the plurality of second receiving structures 2011 are respectively disposed at intervals in a horizontal direction, forming a plurality of third receiving cavities that are distributed at intervals. In some embodiments, the number of third receiving cavities is two.

With continued reference to fig. 2, the upper case assembly 200 further includes a thermal insulation upper case, a fourth opening is formed at the bottom of the thermal insulation upper case, the thermal conduction upper case is disposed in the thermal insulation upper case, and the second accommodating structure 2011 protrudes out of the thermal insulation upper case through the fourth opening. The upper thermal insulation box is made of a material with thermal insulation performance, for example, the upper thermal insulation box can be a foam box made of foam. The shape of the inner cavity of the heat-insulating upper box is matched with the shape of the heat-conducting upper box, so that the gap between the heat-conducting upper box and the heat-insulating upper box is reduced, and the heat-insulating effect is further improved. The heat insulation upper box not only can isolate heat conduction to avoid cold quantity of the dry ice in the heat conduction upper box from overflowing, but also can protect operators from being frostbitten by the excessively low temperature of the dry ice.

Further, the upper thermal insulation box comprises a thermal insulation ring sleeve 202 and a thermal insulation box cover 203 which are buckled, the thermal insulation ring sleeve 202 is sleeved outside the upper metal box 201, and the thermal insulation box cover 203 is detachably connected in the second opening to seal the second opening. It should be noted that, in some embodiments, the thermal insulation box cover 203 may replace the metal upper cover to block the second opening, and in other embodiments, the thermal insulation box cover 203 and the metal upper cover may exist at the same time, at this time, the metal upper cover is embedded into the thermal insulation box cover 203, and the thermal insulation box cover 203 is used to avoid the cold of the dry ice flowing out from the metal upper cover.

With continued reference to fig. 2, the thermal insulation box cover 203 includes a cover portion and a plug portion 2031, the size of the cover portion is larger than the size of the plug portion 2031, and the size of the plug portion 2031 is the same as the opening size of the thermal insulation ring cover 202. The plug-in portion 2031 is inserted into the top opening of the insulation ring sleeve 202, so that the second opening can be plugged, and the insulation box cover 203 and the insulation ring sleeve 202 can be clamped in a nested manner by the plug-in portion 2031, so that the insulation box cover is prevented from conducting heat inside and outside the whole freezing embedding device and simultaneously has a fixing effect.

In order to avoid that when the user lifts up the upper cover assembly, the metal lower case 101 is separated from the heat insulation ring sleeve 202, the lower half part of the heat insulation ring sleeve 202 is tightened and reduced in the inside thereof, so that a first step surface is formed at the inner wall surface of the heat insulation ring sleeve 202, the same bottom tightening of the metal upper case 201 is reduced, and a second step surface is formed at the outer wall surface of the metal upper case 201, and the second step surface is abutted with the first step surface. The cooperation of the first step surface and the second step surface can improve the assembly stability of the metal lower box 101 and the heat insulation ring sleeve 202, and prevent the metal lower box 101 from being separated from the lower side of the heat insulation ring sleeve 202 when a user picks up the upper cover assembly.

The heat preservation lower box 103 and the heat preservation upper box are matched to be used, the heat conduction upper box and the heat conduction lower box can be completely covered, in order to improve the tightness of the heat preservation lower box 103 and the heat preservation upper box after being assembled, buckling grooves 1031 are formed in one of the heat preservation lower box 103 and the heat preservation upper box along the circumferential direction, buckling protrusions 2021 are formed in the other of the heat preservation lower box 103 and the heat preservation upper box along the circumferential direction, and the buckling protrusions 2021 are inserted into the buckling grooves 1031. The cooperation of the buckling grooves 1031 and the buckling protrusions 2021 can enable the heat-insulation lower box 103 and the heat-insulation upper box to be nested and clamped, and the fixing function is achieved while the heat conduction inside and outside the whole freezing embedding device is prevented. In some embodiments, the snap grooves 1031 are annular grooves and the snap protrusions 2021 are annular protrusions.

The procedure for tissue section using the frozen cassette apparatus was as follows:

1. preparation before embedding:

any one of block dry ice, columnar dry ice and powdery dry ice is filled in the metal lower case 101 sleeved with the heat-insulating lower case 103, and the metal lower case 101 is buckled with the metal lower cover 102, so that the lower case assembly 100 is formed.

Any one of block dry ice, columnar dry ice and powdery dry ice is filled in the metal upper case 201 covered with the insulating ring cover 202, and the insulating case cover 203 is covered on the metal upper case 201, thereby forming the upper case assembly 200.

The upper box assembly 200 is buckled on the lower box assembly 100, and the upper box assembly 200 and the lower box assembly 100 are placed for more than 5 minutes after being assembled, so that the internal temperature of the whole device reaches to-80 ℃.

2. Tissue embedding:

2/3 of OCT reagent (Sakura, USA) is added to each of the two metal embedding cassettes 300, after the tissue is isolated, the tissue is wiped with sterile gauze to clean the blood, the tissue is placed into the metal embedding cassette 300 in a downward tangential direction, and finally the metal embedding cassette 300 is filled with the OCT reagent.

The upper case assembly 200 is picked up, two metal embedding cases 300 containing OCT and tissue are placed in two receiving grooves of the lower case assembly 100, respectively, and then the upper case assembly 200 is put back onto the lower case assembly 100, and corresponding protrusions on the upper case assembly 200 will automatically be clamped above the metal embedding cases 300.

After 5 minutes of rest, the upper cassette assembly 200 is lifted and the OCT in the metal embedding cassette 300 is observed to have solidified Cheng Baise solids, thus completing the embedding.

3. Tissue section quality inspection:

the embedded metal embedding box 300 is taken up, and the two sides of the metal embedding box 300 are gently broken outwards, so that the tissue block can be demolded. After the tissue mass was equilibrated in the frozen microtome for 30 minutes, the pieces were trimmed and sectioned. Finally, hematoxylin-eosin staining (hematoxylin-eosin staining) was performed, followed by observation of the tissue section quality under a 10-fold microscope.

Compared with the traditional plastic embedding box embedding method, the frozen embedding box device provided by the utility model can be used for obtaining tissue slices without ice crystals and with good quality.

The freezing embedding box device provided by the utility model has the following advantages:

1. the freezing embedding box device has extremely high portability, and a user can easily carry the freezing embedding box device to any working environment.

2. The freezing embedding box device can effectively maintain the temperature in the device to be-80 ℃, and the maintaining time of the temperature is up to 6-8 hours, so that tissue embedding is not limited by time.

3. The freezing embedding box device has high operation friendliness, no dangerous liquid exists, and the heat preservation box formed by the heat preservation upper box and the heat preservation lower box 103 can effectively protect the use safety of a user.

4. The freezing embedding box device can reduce the temperature of the tissue and the metal embedding box 300 to minus 20 ℃ within 2 minutes, and can effectively prevent ice crystals from generating.

It is to be understood that the above examples of the present utility model are provided for clarity of illustration only and are not limiting of the embodiments of the present utility model. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the utility model are desired to be protected by the following claims.

Claims (10)

1. A freezing and embedding device, comprising:

a lower box assembly (100), wherein the lower box assembly (100) comprises a heat-conducting lower box, a first accommodating cavity for accommodating a refrigerant is formed in the heat-conducting lower box, and a first accommodating structure (1021) is formed on the top surface of the heat-conducting lower box;

go up box subassembly (200), go up box subassembly (200) cartridge is in on lower box subassembly (100), go up box subassembly (200) including the heat conduction and go up the box, be formed with in the heat conduction and hold the second of refrigerant holds the chamber, be formed with second holding structure (2011) on the bottom surface of heat conduction on the box, second holding structure (2011) with first holding structure (1021) cooperate and form the third that is used for holding metal embedding box (300) and holds the chamber.

2. The frozen embedding apparatus according to claim 1, it is characterized in that the method comprises the steps of,

the heat conduction lower box comprises a metal lower box (101) and a metal lower cover (102), a first opening is formed at the top of the metal lower box (101), the metal lower cover (102) is buckled on the first opening, and the first accommodating structure (1021) is formed on the top surface of the metal lower cover (102);

and/or, the heat conduction upper box comprises a metal upper box (201), a second opening is formed at the top of the metal upper box (201), the second opening can be in a blocking state, and the second accommodating structure (2011) is formed on the bottom surface of the metal upper box (201).

3. The frozen embedding apparatus according to claim 2, it is characterized in that the method comprises the steps of,

the lower box assembly (100) further comprises a heat-insulating lower box (103), a third opening is formed at the top of the heat-insulating lower box (103), and the heat-conducting lower box is arranged in the heat-insulating lower box (103) through the third opening;

the upper box assembly (200) further comprises a heat-insulating upper box, a fourth opening is formed in the bottom of the heat-insulating upper box, the heat-conducting upper box is arranged in the heat-insulating upper box, and the second accommodating structure (2011) protrudes out of the heat-insulating upper box through the fourth opening.

4. A freeze-embedding device as claimed in claim 3, wherein,

the heat-insulating upper box comprises a heat-insulating ring sleeve (202) and a heat-insulating box cover (203), wherein the heat-insulating ring sleeve (202) is sleeved outside the metal upper box (201), and the heat-insulating box cover (203) is detachably connected in the second opening so as to seal the second opening.

5. The frozen embedding apparatus according to claim 4, it is characterized in that the method comprises the steps of,

the insulation box cover (203) is convexly provided with a plug part (2031), and the plug part (2031) is inserted into the top opening of the insulation ring sleeve (202).

6. The frozen embedding apparatus according to claim 4, it is characterized in that the method comprises the steps of,

the inner wall surface of the heat preservation ring sleeve (202) is provided with a first step surface, the outer wall surface of the metal upper box (201) is provided with a second step surface, and the second step surface is abutted with the first step surface.

7. A freeze-embedding device as claimed in claim 3, wherein,

one of the heat-insulating lower box (103) and the heat-insulating upper box is provided with a buckling groove (1031) along the circumferential direction, the other is provided with a buckling protrusion (2021) along the circumferential direction, and the buckling protrusion (2021) is inserted into the buckling groove (1031).

8. A freeze-embedding device as claimed in claim 3, wherein,

the metal lower cover (102) is provided with a first annular side wall (1022) buckled outside the metal lower box (101), an annular groove (1032) is formed at the opening of the heat preservation lower box (103), and the first annular side wall (1022) is arranged in the annular groove (1032).

9. The frozen embedding apparatus according to claim 1, it is characterized in that the method comprises the steps of,

one of the first accommodating structure (1021) and the second accommodating structure (2011) is an accommodating groove, the other is a protrusion, the protrusion is arranged on the upper part of the accommodating groove and seals the notch of the accommodating groove, and the protruding outer wall surface and the inner wall surface of the accommodating groove enclose to form the third accommodating cavity.

10. The freezing and embedding device according to any one of claims 1 to 9, wherein,

the number of the first accommodating structures (1021) and the number of the second accommodating structures (2011) are multiple, and the first accommodating structures (1021) and the second accommodating structures (2011) are arranged in a one-to-one correspondence mode so as to form multiple third accommodating cavities.