CN219417794U - Adapter of flexible bag of bioreactor easy to connect - Google Patents
Adapter of flexible bag of bioreactor easy to connect Download PDFInfo
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- CN219417794U CN219417794U CN202320437496.3U CN202320437496U CN219417794U CN 219417794 U CN219417794 U CN 219417794U CN 202320437496 U CN202320437496 U CN 202320437496U CN 219417794 U CN219417794 U CN 219417794U
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- connecting piece
- flexible bag
- light
- bioreactor
- hole
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Abstract
An adapter of a flexible bag of a bioreactor which is easy to connect is arranged at the bottom of the flexible bag provided with a through hole and comprises an optical sensing film, an optical fiber, a lens assembly, an inner connecting piece, a plurality of magnetic pieces and an outer connecting piece; the optical sensing film is directly adhered to the upper surface of the light hole, the outer connecting piece is detachably connected to the lower part of the inner connecting piece through magnetic force, the magnetic pieces are fixed on the inner connecting piece and the outer connecting piece and tightly attached in pairs, the lens component is arranged in the outer connecting piece, and the optical fiber is led into the outer connecting piece and connected with the lens component; the excitation light is input along the optical fiber and reflected by the lens component, then reaches the optical sensing film through the light transmission hole, the excitation light irradiates the optical sensing film to generate emission light with the intensity related to the information to be detected in the flexible bag, and the emission light is conducted by the original optical path and output through the optical fiber to finish detection. The utility model has the advantages of convenient installation and operation, long service life, avoiding the interference of bubble residues and stabilizing the optical signal intensity.
Description
Technical Field
The utility model relates to a biological reaction system, in particular to an adapter of a flexible bag of a bioreactor which is easy to connect, and belongs to the technical field of biology.
Background
The bioreactor is one of important equipment in bioengineering, and is mainly used in the fields of cell culture, pharmaceutical production, animal and plant tissue culture, microbial fermentation production, biological product preparation and the like. A single-use bioreactor (single-use bioreactor) is a bioreactor using a disposable reaction bag instead of a culture vessel made of stainless steel or glass. Disposable bioreactors typically include a disposable flexible bag made of plastic and a permanent rigid support structure (e.g., a cradle or steel cylinder) surrounding the disposable flexible bag. The disposable flexible reaction bag belongs to disposable use, and is replaced after each experiment or production stage, so that the cleaning and sterilization after the use of the permanent bioreactor and the related process cleaning and authentication process are avoided, and the disposable flexible reaction bag has the advantages of shortening the downtime and the turnover time and increasing the production conversion flexibility; in addition, the disposable bioreactor has simple structure and small occupied area, thus reducing fixed investment and operation cost, saving operation space and energy and labor consumption and shortening new product development and marketing time for research and development and pilot-scale. Disposable bioreactors have therefore been increasingly used in modern biopharmaceutical processes over the past few years and have expanded towards a wider field of application.
In order to monitor the reaction environment conditions, such as PH, dissolved oxygen, etc., in the disposable bioreactor in real time, a sensor, including an optical sensor, is typically provided in the disposable reaction bag. The optical sensor comprises fluorescent substances, is placed at the bottom of the disposable reaction bag and is in the environment of reactants, light with a certain wavelength is injected from the outside, the light is reflected from the optical sensor, and information in the disposable reaction bag is obtained from analysis of the reflected light. The measures taken to introduce and withdraw test light into and out of a sealed bioreactor have therefore become a technical problem that must be addressed, which has been made more difficult with disposable bags made of flexible materials.
The prior art solves the problems by adopting a sensing film fixing component combined on a flexible bag and an optical adapter component arranged outside the flexible bag; the sensing film fixing part comprises a bag attachment part welded to the flexible bag and a sensor attachment part pressed on the bag attachment part, the optical sensing film is arranged on the sensor attachment part and is in direct contact with reactants in the flexible bag, and an optical fiber and lens assembly part for transmitting excitation light is fixedly connected on the optical adapter part; the optical adapter component is rotatably connected to the sensing film fixing component, and the two components are combined together through the cooperation of locking devices on the two components, so that the optical fiber is communicated with the optical sensing film. However, this technique has the following drawbacks:
firstly, the optical fiber and the lens component for transmitting the excitation light are all arranged on the optical adapter component, when the disposable reaction bag is replaced each time or when the optical fiber or the lens component is required to be maintained and replaced, the optical adapter component is detached from the sensor film fixing component in a rotating mode, and the locking device is complex in structure and high in locking strength, so that the rotary type optical adapter component is troublesome in assembling and disassembling operation and easy to damage or even break the long optical fiber due to rotary stress, the signal strength between the optical sensor film and the lens is changed, and the service life of the optical adapter component is shortened.
Secondly, the optical sensing film on the sensing film fixing part is arranged in the sensor attachment part which is pressed on the bag attachment part, so that the pressure combination structure is very small and complex, the assembly is inconvenient, and the solution in the flexible bag is likely to leak from a gap between the sensor attachment part and the bag attachment part to enter the light through hole; in addition, the optical sensing film is in the recessed region of the bag attachment member, and thus residual bubbles are easily collected therein, which factors affect the accuracy of measuring the optical signal.
Disclosure of Invention
The utility model aims to overcome the defects of the prior art, and provides an adapter of a flexible bag of an easily-connected bioreactor, wherein an optical fiber and a lens assembly are arranged on an outer connecting piece, and the outer connecting piece and an inner connecting piece connected to the flexible bag are tightly locked through magnetic force adsorption, so that the influence of rotation stress on the optical fiber during installation is avoided, and an optical sensing film is directly adhered to the inner connecting piece in an adhering mode, so that the risks of bubble residue and liquid leakage at the optical sensing film are avoided, and the effects of convenience in installation operation, stability of optical signal intensity and prolonged service life of devices are achieved.
Based on the above purpose, the technical scheme provided by the utility model is as follows:
an adapter of a bioreactor flexible bag easy to connect is arranged at the bottom of the flexible bag, and the bottom is provided with a through hole, and is characterized in that: the adapter comprises an inner connecting piece, an optical sensing film, an optical fiber, a lens assembly, a plurality of magnetic pieces and an outer connecting piece; the inner connecting piece is fixedly connected with the bottom of the flexible bag into a whole, a light hole opposite to the through hole is formed in the center of the inner connecting piece, the optical sensing film is directly stuck on the light hole and carries fluorescent substances capable of detecting information to be detected in the flexible bag, the outer connecting piece is detachably connected below the inner connecting piece through magnetic force, the magnetic pieces are fixed on the inner connecting piece and the outer connecting piece and are tightly attached in pairs, the lens assembly is arranged in the outer connecting piece, and the optical fibers are led into the outer connecting piece and are connected with the lens assembly;
excitation light is input along the optical fiber and reflected by the lens assembly, then reaches the optical sensing film through the light transmission hole, the excitation light irradiates the optical sensing film to generate emission light with the intensity related to information to be detected in the flexible bag, and the emission light is conducted by an original light path and is output through the optical fiber to finish detection.
As a further improvement, the inner connecting piece comprises a disc and a plurality of fixing grooves; the disc with flexible bag moulds and welds to be connected, the light trap is used for through excitation light and emission light, sets up in the center of this disc, the downside downward bulge of disc has the round platform, the fixed slot downwards protruding ground fixed connection in the downside of round platform, part the magnetic part is fixed in this fixed slot.
As a further improvement, the inner connecting piece is positioned at the outer side of the bottom of the flexible bag, the upper side surface of the disc is in plastic welding connection with the bottom of the flexible bag, and the optical sensing film is clamped between the light hole of the disc and the through hole of the flexible bag.
As a further improvement, the inner connecting piece is positioned at the inner side of the bottom of the flexible bag, the part of the lower side surface of the disc positioned at the outer side of the circular table is in plastic welding connection with the inner part of the flexible bag, and the circular table and the fixing groove are downwards protruded below the flexible bag.
As a further improvement, the outer connecting piece comprises a flat cylindrical part, an inner cavity for placing the lens assembly is arranged in the flat cylindrical part, the upper part of the inner cavity is opened and is opposite to the light hole of the inner connecting piece, an optical fiber hole for installing the optical fiber is arranged in the flat cylindrical part and is communicated with the inner cavity, a plurality of magnetic piece grooves are formed in the upper side face of the flat cylindrical part, and part of magnetic pieces are fixed in the magnetic piece grooves.
As a further improvement, the fixing grooves are uniformly distributed along the circumference, and the positions of the magnetic piece grooves correspond to the positions of the fixing grooves.
As a further improvement, an outer conical surface is arranged at the joint of the periphery of the round table and the round disc, an inner conical surface is arranged at the periphery of the upper side surface of the oblate cylinder part, and the inner conical surfaces are in contact fit to position the inner connecting piece and the outer connecting piece relatively.
As a further improvement, the position of the inner conical surface corresponds to the position of the outer conical surface, and the taper is the same as the taper of the outer conical surface.
As a further improvement, the magnetic member is fixed to the inner and outer connection members with an adhesive.
Compared with the prior art, the utility model has the following effects:
1) The optical fiber and the lens component are arranged on the outer connecting piece, the outer connecting piece and the inner connecting piece are locked through magnetic force adsorption, the optical fiber is long and is connected with the testing instrument, the influence of loading and unloading rotation stress on the optical fiber in the prior art is avoided through the magnetic force adsorption direct locking mode, the risk of damage and even fracture on the optical fiber is reduced, and the effects of being convenient to operate and prolonging the service life are achieved.
2) The optical sensing film is directly adhered to the light hole of the inner connecting piece in an adhering mode and is on the same plane with the bottom of the flexible bag, so that bubble residues and liquid leakage are completely avoided, and the effects of simple and convenient installation structure and stable optical signal intensity are achieved.
In a word, the utility model has the advantages of convenient installation and operation, long service life, avoiding the interference of bubble residues and stabilizing the optical signal intensity.
Drawings
Fig. 1 is an exploded view of the structure of the present utility model.
Fig. 2 is a bottom view of the present utility model.
Fig. 3 is a cross-sectional view A-A of fig. 2.
Fig. 4 is a schematic view of an inner connector.
Fig. 5 is a bottom view of the outer connector.
Fig. 6 is a B-B cross-sectional view of fig. 5.
Fig. 7 is a schematic diagram of the optical path of the present utility model.
In the figure:
01-flexible bag, 02-through hole, 1-inner connecting piece, 11-disc, 12-round table, 13-light hole, 14-outer conical surface, 15-fixed groove, 2-magnetic piece, 3-lens component, 4-outer connecting piece, 41-oblate cylinder part, 42-magnetic piece groove, 43-inner conical surface, 44-inner cavity, 45-optical fiber hole, 5-optical fiber, 6-optical sensing film.
Detailed Description
The utility model is further illustrated in the following figures and examples, which are not intended to limit the scope of the utility model as claimed.
The utility model is used for guiding out excitation light and emission light from the disposable flexible bag when the optical sensor is used for monitoring the reaction conditions in the bioreactor.
Referring to fig. 7, the adaptor of the flexible bag of the easily-connected bioreactor is installed at the bottom of the flexible bag 01, and the bottom of the flexible bag 01 is provided with a through hole 02 through which light and reactants can pass.
Example 1
Referring to fig. 1, 2 and 3, the adaptor of the present embodiment includes an optical sensing film 6, an optical fiber 5, a lens assembly 3, an inner connector 1, a plurality of magnetic members 2 and an outer connector 4; wherein, interior connecting piece 1 with the bottom fixed connection of flexible bag 01 becomes an organic wholely, optical sensing membrane 6 directly paste on interior connecting piece 1, outer connecting piece 4 detachably pass through magnetic force adsorb connect in interior connecting piece 1's below, lens assembly 3 set up in the inside of this outer connecting piece 4, optic fibre 5 lets in outer connecting piece 4 and with lens assembly 3 connects.
Referring to fig. 4, the inner connector 1 is located at the outer side of the bottom of the flexible bag 01, and includes a disc 11 and a plurality of fixing grooves 15. The upper side of the disc 11 is connected to the flexible bag 01, which flexible bag 01 is usually made of plastic, and the connection is usually made of plastic welding. The center of the disc 11 is provided with a light hole 13, and the light hole 13 is opposite to the through hole 02 of the flexible bag 01 so as to pass excitation light and emit light. The lower side of the disc 11 protrudes downwards to form a round table 12, and an outer conical surface 14 is arranged at the joint of the periphery of the round table 12 and the disc 11 and is used for positioning the outer connecting piece 4. In this embodiment, the number of the fixing grooves 15 is four, and the four fixing grooves 15 are fixedly connected to the lower side surface of the circular table 12 in a downward protruding manner and are uniformly distributed along the circumference; the lower side of the fixed slot 15 is opened, and a magnetic piece 2 is fixed in each slot.
Referring to fig. 5 and 6, the outer connecting member 4 includes a flat cylindrical portion 41; the interior of the cylindrical portion 41 is provided with an inner cavity 44 for placing the lens assembly 3, and the upper portion of the inner cavity 44 is opened to face the light transmission hole 13 of the inner connector 1 to pass the excitation light and emit the light. The interior of the oblate cylindrical portion 41 is also provided with an optical fiber hole 45 for mounting the optical fiber 5, the optical fiber hole 45 communicating with the inner cavity 44. The upper side of the oblate column 41 is provided with a plurality of magnetic element grooves 42, in this embodiment, the number of the magnetic element grooves 42 is four, the positions of the magnetic element grooves 42 correspond to the four fixing grooves 15 of the inner connecting piece 1, and a magnetic element 2 is fixed in each magnetic element groove 42. The periphery of the upper side surface of the outer connecting piece 4 is provided with an inner conical surface 43, and the inner conical surface 43 is attached with the outer conical surface 14 so as to position the inner connecting piece 1 and the outer connecting piece 4 relatively; the position of the inner conical surface 43 corresponds to the position of the outer conical surface 14, and the taper of the inner conical surface 43 is the same as the taper of the outer conical surface 14.
Referring to fig. 1 and 3 in combination, eight magnetic members 2 are respectively fixed in the fixing groove 15 of the inner connecting member 1 and the magnetic member groove 42 of the outer connecting member 4, and an adhesive is generally used to fix the magnetic members 2 in the fixing groove 15 or the magnetic member groove 42.
When the outer connecting piece 4 is connected with the inner connecting piece 1, the outer connecting piece 4 and the inner connecting piece 1 can be quickly connected by the magnetic attraction of the magnetic pieces 2 only by tightly attaching the magnetic pieces 2 in pairs, so that the operation is very simple and convenient; the magnetic force adsorption direct locking mode avoids the influence of loading and unloading rotation stress on the optical fiber 5 in the prior art, reduces the risk of damage or even fracture to the optical fiber 5, and achieves the effects of being convenient to operate and prolonging the service life. Meanwhile, the round table 12 of the inner connector 1 goes deep into the outer connector 4, the outer conical surface 14 of the round table is closely attached to the inner conical surface 43, and the outer connector 4 is accurately positioned on the inner connector 1 by utilizing the guiding function of the conical surface, so that the accuracy of a detection light path is ensured, and the detection precision is improved.
The optical sensing film 6 carries a fluorescent substance capable of detecting information to be detected in the flexible bag 01, is directly adhered on the light hole 13 of the inner connector 1 in an adhering manner, and is sandwiched between the light hole 13 of the disc 11 and the through hole 02 of the flexible bag 01, please refer to fig. 3 and 7 in combination. Therefore, after the disc 11 and the flexible bag 01 are integrally connected through plastic welding, the optical sensing film 6 and the bottom of the flexible bag 01 are on the same plane, so that the occurrence of bubble residue and liquid leakage is completely avoided, and the effect of stabilizing the optical signal intensity is achieved.
Referring to fig. 3, the lens assembly 3 is disposed in the inner cavity 44 of the oblate cylindrical portion 41 of the outer connector 4 and aligned with the light hole 13 of the inner connector 1 and the through hole 02 of the flexible bag 01. The optical fiber 5 is disposed in a fiber hole 45 in the outer connector 4 and is connected to the lens assembly 3.
The working principle of the embodiment is as follows:
referring to fig. 7, excitation light is input along the optical fiber 5 and reflected by the lens assembly 3, and then reaches the optical sensing film 6 through the light transmission hole 13, the excitation light irradiates the optical sensing film 6 to generate emission light with intensity related to information to be detected in the flexible bag 01, and the emission light is conducted by an original optical path and output by the optical fiber to complete detection.
Example two
The structure of this embodiment is substantially the same as that of the first embodiment except that: the inner connecting piece 1 is located at the inner side of the bottom of the flexible bag 01, the part of the lower side surface of the disc 11 located at the outer side of the round table 12 is in plastic welding connection with the inner side surface of the flexible bag 01, and the round table 12 and the fixing groove 15 protrude downwards below the flexible bag 01.
When the optical sensor is in operation, excitation light is input along the optical fiber and reflected by the lens component 3, then passes through the light transmission hole 13 to reach the optical sensor film 6, the optical sensor film 6 carries fluorescent substances capable of detecting information to be detected in the flexible bag 01 and is directly contacted with reactants, the intensity of emitted light generated by the excitation light irradiation of the optical sensor film 6 is related to information signals to be detected in the flexible bag 01, and the emitted light is conducted by an original light path and is output by the optical fiber to finish detection.
The foregoing description is only of the preferred embodiments of the utility model and is not intended to limit the scope of the utility model. Equivalent changes and modifications are intended to be within the scope of the present utility model.
Claims (9)
1. An adapter of a bioreactor flexible bag easy to connect is arranged at the bottom of the flexible bag, and the bottom is provided with a through hole, and is characterized in that: the adapter comprises an optical sensing film, an optical fiber, a lens assembly, an inner connecting piece, a plurality of magnetic pieces and an outer connecting piece; the inner connecting piece is fixedly connected with the bottom of the flexible bag into a whole, a light hole opposite to the through hole is formed in the center of the inner connecting piece, the optical sensing film is directly stuck on the light hole, the optical sensing film carries fluorescent substances capable of detecting information to be detected in the flexible bag, the outer connecting piece is detachably connected below the inner connecting piece through magnetic force, the magnetic pieces are fixed on the inner connecting piece and the outer connecting piece and are tightly attached in pairs, the lens component is arranged in the outer connecting piece, and the optical fibers are led into the outer connecting piece and are connected with the lens component;
excitation light is input along the optical fiber and reflected by the lens assembly, then reaches the optical sensing film through the light transmission hole, the excitation light irradiates the optical sensing film to generate emission light with the intensity related to information to be detected in the flexible bag, and the emission light is conducted by an original light path and is output through the optical fiber to finish detection.
2. The adaptor for a flexible bag of an attachable bioreactor of claim 1, wherein: the inner connecting piece comprises a disc and a plurality of fixing grooves; the disc with flexible bag moulds and welds to be connected, the light trap is used for through excitation light and emission light, sets up in the center of this disc, the downside downward bulge of disc has the round platform, the fixed slot downwards protruding ground fixed connection in the downside of round platform, part the magnetic part is fixed in this fixed slot.
3. The adaptor for a flexible bag of an attachable bioreactor of claim 2, wherein: the inner connecting piece is positioned at the outer side of the bottom of the flexible bag, the upper side surface of the disc is in plastic welding connection with the bottom of the flexible bag, and the optical sensing film is clamped between the light transmission hole of the disc and the through hole of the flexible bag.
4. The adaptor for a flexible bag of an attachable bioreactor of claim 2, wherein: the inner connecting piece is positioned at the inner side of the bottom of the flexible bag, the part of the lower side surface of the disc positioned at the outer side of the circular table is in plastic welding connection with the inner part of the flexible bag, and the circular table and the fixing groove are downwards protruded below the flexible bag.
5. The adaptor for a flexible bag of an attachable bioreactor of claim 2, wherein: the outer connecting piece include oblate column portion, the inside of this oblate column portion is equipped with and is used for placing the inner chamber of lens subassembly, and the upper portion of this inner chamber is opened, just to the light trap of interior connecting piece, the inside of oblate column portion is equipped with and is used for installing the optic fibre hole of optic fibre, this optic fibre hole with the inner chamber communicates with each other, the upside of oblate column portion is equipped with a plurality of magnetism spare grooves, and partial magnetism spare is fixed in this magnetism spare inslot.
6. The adapter for a flexible bag of an attachable bioreactor of claim 5, wherein: the fixing grooves are uniformly distributed along the circumference, and the positions of the magnetic piece grooves correspond to the positions of the fixing grooves.
7. The adapter for a flexible bag of an attachable bioreactor of claim 5, wherein: the periphery of the round table and the joint of the round disc are provided with outer conical surfaces, the periphery of the upper side face of the oblate cylindrical part is provided with inner conical surfaces, and the inner conical surfaces are in contact fit to position the inner connecting piece and the outer connecting piece in relative positions.
8. The adapter for a flexible bag of an attachable bioreactor of claim 7, wherein: the position of the inner conical surface corresponds to the position of the outer conical surface, and the taper is the same as that of the outer conical surface.
9. The adaptor for a flexible bag of an attachable bioreactor of claim 1, wherein: the magnetic piece is fixed on the inner connecting piece and the outer connecting piece by adopting an adhesive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320437496.3U CN219417794U (en) | 2023-03-09 | 2023-03-09 | Adapter of flexible bag of bioreactor easy to connect |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320437496.3U CN219417794U (en) | 2023-03-09 | 2023-03-09 | Adapter of flexible bag of bioreactor easy to connect |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219417794U true CN219417794U (en) | 2023-07-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320437496.3U Active CN219417794U (en) | 2023-03-09 | 2023-03-09 | Adapter of flexible bag of bioreactor easy to connect |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219417794U (en) |
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2023
- 2023-03-09 CN CN202320437496.3U patent/CN219417794U/en active Active
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