CN219417488U

CN219417488U - Human immunodeficiency virus HIV1+2 urine antibody detection kit

Info

Publication number: CN219417488U
Application number: CN202223277629.XU
Authority: CN
Inventors: 方会来
Original assignee: Tianjin Bremen Bioengineering Co ltd
Current assignee: Tianjin Bremen Bioengineering Co ltd
Priority date: 2022-12-07
Filing date: 2022-12-07
Publication date: 2023-07-25
Anticipated expiration: 2032-12-07

Abstract

The utility model relates to a human immunodeficiency virus HIV1+2 urine antibody detection kit, which comprises a reaction section, wherein the reaction section is in a strip-shaped hollow flat box shape, and test paper is arranged in the reaction section; the bottom of the sampling box is provided with an opening, a movable plug is inserted into the opening, one end of the movable plug falls into the sampling box, one end of the movable plug falling into the sampling box extends to the circumferential side to form a blocking edge, and the blocking edge prevents the movable plug from falling out of the sampling box; the upper part of the movable plug is provided with a liquid inlet hole, and the liquid inlet hole falls below the baffle edge; pulling the movable plug to the lowest part of the sampling box, and exposing the liquid inlet out of the sampling box. The utility model is operated by moving the plug to absorb liquid, storing the liquid into the sampling box after pressing, and then reacting on the test paper, thereby avoiding the use of consumable materials such as a suction pipe, a dropper and the like, improving the operation efficiency, saving the time and avoiding the occurrence of pollution or error taking and other problems in liquid transfer.

Description

Human immunodeficiency virus HIV1+2 urine antibody detection kit

Technical Field

The utility model relates to the technical field of human immunodeficiency virus detection, in particular to a human immunodeficiency virus HIV1+2 urine antibody detection kit.

Background

The colloidal gold immune binding experiment is a new solid phase labeling immunoassay technology developed on the basis of an enzyme immune binding experiment, and is divided into a gold immune percolation experiment and a gold immune chromatography experiment according to different devices. The gold immunofiltration method is to fix antigen on fiber membrane, pad water absorbing plate under the membrane, drop sample on the membrane during test, wash with solution after complete absorption, then add gold labeling solution, wash again, finally observe the result. Gold immunochromatography uses colloidal gold as a marker, and its detection principle is basically the same as that of gold immunofiltration, except that the movement of liquid is not through straight flow but lateral flow based on chromatography. Various reagents are fixed on the same test strip in a strip shape, a sample to be detected is added at one end of the test strip, after one reagent is dissolved, the reagent is percolated and moves on the chromatographic strip through capillary action and is contacted with the other reagent on the membrane, and a specific immune reaction occurs between an object to be detected in the sample and a receptor (antigen or antibody) aiming at the object to be detected on the chromatographic material. In the chromatographic process, immune complex is trapped and accumulated in a certain area (detection zone) of the chromatographic material, and visual color development results are obtained through visual colloidal gold; the free label passes through the detection zone to separate from the bound label.

The gold-labeled detection reagent is widely used as an HIV gold-labeled detection reagent, and the specific method is that an HIV antigen is used for labeling a gold colloid, the first reaction is combined with an HIV antibody, and then the combined with the HIV antigen immobilized on a membrane to form an antigen gold colloid-antibody-antigen complex. The specificity of the antigen gold label improves the detection sensitivity and specificity.

The method has the advantages of simple operation, short completion time, very straight judgment result through color development, long-term storage at room temperature due to the dry reagent (the effective service life is as long as one year to one half year). The HIV antigen gold-labeled reagent contains two types of HIV-1 and HIV-2 antigens, when the HIV-1 and HIV-2 antigens are coated on a nitrocellulose membrane in a mixing way, when a positive reaction occurs, two red lines appear on the membrane, one is a control line and the other is a reaction line, at the moment, the possibility of HIV infection can be primarily judged, but further confirmation detection is needed to judge whether the HIV is HIV-1 or HIV-2 infection, and finally a detection report is given.

Chinese utility model patent name: a three-line rapid detection plate for Human Immunodeficiency Virus (HIV) 1+2 type antibody, grant bulletin No. CN2501049Y, is used and operation steps are that firstly, a packaging bag containing the HIV1+2 type antibody detection plate is torn, the detection plate is taken out and placed on an experiment table horizontally, 2-3 drops of samples (70-100 microliters) are sucked by a plastic suction tube and added into a sample hole, and the experimental result can be observed after about 15-30 minutes. The problems are: the operation is performed by means of a suction pipe, so that consumable materials are wasted, and the procedure is complex; the liquid transfer has the problems of pollution, error taking and the like.

Therefore, a human immunodeficiency virus HIV1+2 urine antibody detection kit is designed to solve the problems.

Disclosure of Invention

The utility model provides a human immunodeficiency virus HIV+urine antibody detection kit for overcoming the defects, which comprises a reaction section, wherein the reaction section is in a strip-shaped hollow flat box shape, and test paper is arranged in the reaction section; the test paper is a film containing an HIV antigen gold-labeled reagent and a mouse IgG gold-labeled reagent, and the middle of the test paper is respectively coated with an HIV-1 antigen, an HIV-2 antigen and a goat anti-mouse IgG antibody in three sections, namely a first reaction line, a second reaction line and a control line;

an inner-outer transparent observation port is formed in the middle of the reaction section, and a transparent baffle is arranged on the observation port; the reaction line, the reaction line and the control line on the test paper can be observed through the observation port; the reaction line and the reaction line are arranged at the upper T end, and the control line is arranged at the lower C end;

the upper end of the reaction section is expanded to form a liquid storage box, and the lower end of the reaction section is expanded to form a sampling box; a liquid storage channel is arranged between the reaction section and the liquid storage box, and the reaction section is communicated with the liquid storage box through the liquid storage channel; a reaction channel is arranged between the reaction section and the sampling box, and the reaction section is communicated with the sampling box through the reaction channel;

the liquid storage box is internally provided with liquid storage filler;

the bottom of the sampling box is provided with an opening, a movable plug is inserted into the opening, one end of the movable plug falls into the sampling box, one end of the movable plug falling into the sampling box extends to the circumferential side to form a blocking edge, and the blocking edge prevents the movable plug from falling out of the sampling box; the upper part of the movable plug is provided with a liquid inlet hole, and the liquid inlet hole falls below the baffle edge; pulling the movable plug to the lowest part of the sampling box, and exposing the liquid inlet out of the sampling box.

Preferably, the upper part of the observation port is marked with T, and the lower part of the observation port is marked with C.

Preferably, the test paper is one of nitrocellulose membrane, glass fiber membrane or non-woven fabric.

Preferably, the liquid storage filler is one or two of a drying agent and absorbent cotton.

Preferably, a sealing ring is arranged at the opening at the bottom of the sampling box.

Preferably, the sampling box, the reaction section and the liquid storage box are integrally formed by plastic injection molding.

The beneficial effects of the utility model are as follows:

according to the HIV1+2 urine antibody detection kit, liquid is absorbed by moving the plug during operation, the liquid is stored in the sampling box after being pressed, and then the liquid reacts on test paper, so that consumable materials such as a suction pipe and a dropper are avoided, the operation efficiency is improved, the time is saved, and the problems of pollution or error in liquid transfer are avoided.

Drawings

FIG. 1 is a perspective view of the present utility model;

FIG. 2 is a front view of the present utility model;

FIG. 3 is a cross-sectional view taken along the direction A-A of FIG. 2;

FIG. 4 is a perspective view of the present utility model;

FIG. 5 is a front view of the present utility model;

FIG. 6 is a cross-sectional view taken along the direction A-A of FIG. 2;

wherein:

1-moving the plug; 2-a sampling box; 3-a reaction section; 4-a viewing port; 5-a liquid storage box; 6-a liquid inlet hole;

7-test paper; 8-a sealing ring; 9-reaction channel; 10-a liquid storage channel; 11-a liquid storage filler;

Detailed Description

The embodiments of the present utility model will be described in detail below with reference to the accompanying drawings.

As shown in FIG. 1, the HIV1+2 urine antibody detection kit comprises a reaction section 3, wherein the reaction section 3 is in a strip-shaped hollow flat box shape, and test paper 7 is arranged in the reaction section 3; the test paper 7 is a film containing an HIV antigen gold-labeled reagent and a mouse IgG gold-labeled reagent, and the middle of the test paper 7 is respectively coated with an HIV-1 antigen, an HIV-2 antigen and a goat anti-mouse IgG antibody in three sections, namely a first reaction line, a second reaction line and a control line;

the middle part of the reaction section 3 is provided with an inside-outside transparent observation port 4, and the observation port 4 is provided with a transparent baffle; the first reaction line, the second reaction line and the control line on the test paper 7 can be observed through the observation port 4; the first reaction line and the second reaction line are arranged at the upper T end, and the control line is arranged at the lower C end;

as shown in fig. 3, the upper end of the reaction section 3 is expanded to form a liquid storage box 5, and the lower end of the reaction section 3 is expanded to form a sampling box 2; a liquid storage channel 10 is arranged between the reaction section 3 and the liquid storage box 5, and the reaction section 3 is communicated with the liquid storage box 5 through the liquid storage channel 10; a reaction channel 9 is arranged between the reaction section 3 and the sampling box 2, and the reaction section 3 is communicated with the sampling box 2 through the reaction channel 9;

the liquid storage box 5 is internally provided with a liquid storage filler 11;

the bottom of the sampling box 2 is provided with an opening, a movable plug 1 is inserted into the opening, one end of the movable plug 1 falls into the sampling box 2, one end of the movable plug 1 falling into the sampling box 2 extends to the circumferential side to form a blocking edge, and the blocking edge prevents the movable plug 1 from falling out of the sampling box 2; the upper part of the movable plug 1 is provided with a liquid inlet hole 6, and the liquid inlet hole 6 falls below the baffle edge; the movable plug 1 is pulled to the lowest part of the sampling box 2, and the liquid inlet hole 6 can be exposed out of the sampling box 2.

As shown in fig. 4, preferably, the upper portion of the viewing port 4 is marked with T, and the lower portion of the viewing port 4 is marked with C.

Preferably, the test paper 7 is one of nitrocellulose membrane, glass fiber membrane or non-woven fabric.

Preferably, the liquid storage filler 11 is one or two of a desiccant or absorbent cotton.

Preferably, a sealing ring 8 is arranged at the opening at the bottom of the sampling box 2.

Preferably, the sampling box 2, the reaction section 3 and the liquid storage box 5 are integrally formed by plastic injection molding.

The using method comprises the following steps:

inserting a movable plug 1 into a sample (blood or urine) to be detected, wherein the movable plug 1 is at the lowest position, a liquid inlet hole 6 on the movable plug 1 absorbs the sample into a sampling box 2, pressing the movable plug 1 at the moment to enable the movable plug 1 to move upwards, enabling the liquid inlet hole 6 to be contracted into the sampling box 1, then horizontally placing, and introducing the sample from the sampling box 2 to a test paper 7 through a reaction channel 9 for reaction;

the test paper 7 is a film containing an HIV antigen gold-labeled reagent and a mouse IgG gold-labeled reagent, and the middle of the test paper 7 is respectively coated with an HIV-1 antigen, an HIV-2 antigen and a goat anti-mouse IgG antibody in three sections, namely a first reaction line, a second reaction line and a control line;

the first reaction line, the second reaction line and the control line can be observed through the observation port 4; if both the first reaction line and the control line are colored, then HIV-1 virus is infected, and if both the second reaction line and the control line are colored, then HIV-2 virus is infected.

Excess liquid is introduced into the liquid storage box 5 through the liquid storage channel 10 and is absorbed by the liquid storage filler 11.

In the description of the present utility model, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present utility model and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art in a specific case.

The foregoing is merely a preferred embodiment of the present utility model and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present utility model, which are intended to be comprehended within the scope of the present utility model.

Claims

1. The human immunodeficiency virus HIV1+2 urine antibody detection kit comprises a reaction section (3), and is characterized in that the reaction section (3) is in a strip-shaped hollow flat box shape, and test paper (7) is arranged in the reaction section (3); the test paper (7) is a film containing an HIV antigen gold-labeled reagent and a mouse IgG gold-labeled reagent, and the middle of the test paper (7) is respectively coated with an HIV-1 antigen, an HIV-2 antigen and a goat anti-mouse IgG antibody in three sections, namely a first reaction line, a second reaction line and a control line;

an inner and outer transparent observation port (4) is formed in the middle of the reaction section (3), and a transparent baffle is arranged on the observation port (4); the first reaction line, the second reaction line and the control line on the test paper (7) can be observed through the observation port (4); the first reaction line and the second reaction line are arranged at the upper T end, and the control line is arranged at the lower C end;

the upper end of the reaction section (3) is expanded to form a liquid storage box (5), and the lower end of the reaction section (3) is expanded to form a sampling box (2); a liquid storage channel (10) is arranged between the reaction section (3) and the liquid storage box (5), and the reaction section (3) is communicated with the liquid storage box (5) through the liquid storage channel (10); a reaction channel (9) is arranged between the reaction section (3) and the sampling box (2), and the reaction section (3) is communicated with the sampling box (2) through the reaction channel (9);

the liquid storage box (5) is internally provided with a liquid storage filler (11);

the bottom of the sampling box (2) is provided with an opening, a movable plug (1) is inserted into the opening, one end of the movable plug (1) falls into the sampling box (2), one end of the movable plug (1) falling into the sampling box (2) extends to the circumferential side to form a blocking edge, and the blocking edge prevents the movable plug (1) from falling out of the sampling box (2); the upper part of the movable plug (1) is provided with a liquid inlet hole (6), and the liquid inlet hole (6) falls below the blocking edge; the movable plug (1) is pulled to the lowest part of the sampling box (2), and the liquid inlet hole (6) can be exposed out of the sampling box (2).

2. The human immunodeficiency virus HIV1+2 urine antibody detection kit according to claim 1, wherein the upper portion of the viewing port (4) is labeled with T, and the lower portion of the viewing port (4) is labeled with C.

3. The HIV1+2 urine antibody detection kit according to claim 2,

the test paper (7) is one of a nitrocellulose membrane, a glass fiber membrane or a non-woven fabric.

4. The human immunodeficiency virus HIV1+2 urine antibody detection kit according to claim 3, wherein the stock solution filler (11) is one or both of a desiccant or absorbent cotton.

5. The human immunodeficiency virus HIV1+2 urine antibody detection kit according to claim 4, wherein a sealing ring (8) is arranged at the bottom opening of the sampling box (2).

6. The human immunodeficiency virus HIV1+2 urine antibody detection kit according to claim 5, wherein the sampling cartridge (2), the reaction section (3) and the liquid storage cartridge (5) are integrally formed by plastic injection molding.