CN219314902U - Nucleic acid extraction and purification device - Google Patents
Nucleic acid extraction and purification device Download PDFInfo
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- CN219314902U CN219314902U CN202221974323.7U CN202221974323U CN219314902U CN 219314902 U CN219314902 U CN 219314902U CN 202221974323 U CN202221974323 U CN 202221974323U CN 219314902 U CN219314902 U CN 219314902U
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Abstract
The utility model discloses a nucleic acid extraction and purification device, which relates to the technical field of nucleic acid extraction, and particularly relates to a nucleic acid extraction and purification device, comprising a base, wherein the upper surface of the base is provided with a mixing box, the upper surface of the mixing box is provided with a first through hole, the inside of the first through hole is provided with a first bearing, the inside of the first bearing is provided with a first shaft lever, and the nucleic acid extraction and purification device is provided with a first motor, a first gear, a second gear, a first shaft lever, a second shaft lever and a stirring rod in a matched manner.
Description
Technical Field
The utility model relates to the technical field of nucleic acid extraction, in particular to a nucleic acid extraction and purification device.
Background
Nucleic acid is a biological macromolecular compound polymerized from a plurality of nucleotides, is one of the most basic substances of life, is widely existed in all animal and plant cells and microorganisms, is often combined with protein to form nucleoprotein, and has different chemical compositions, nucleotide arrangement sequences and the like, and can be divided into ribonucleic acid and deoxyribonucleic acid according to the different chemical compositions, and the method for extracting the nucleic acid by a general device comprises the following steps: DNA extraction and purification methods and RNA extraction and purification methods.
The mixing efficiency of the existing nucleic acid extraction and purification device is low, the purification efficiency is affected, and meanwhile, when the existing nucleic acid extraction and purification device is heated, the heating is not uniform enough, so that the extraction purity of nucleic acid is affected.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the utility model provides a nucleic acid extraction and purification device, which solves the problems in the prior art.
(II) technical scheme
In order to achieve the above purpose, the utility model is realized by the following technical scheme: the utility model provides a nucleic acid extraction purification device, includes the base, the upper surface of base is provided with the mixing box, first through-hole has been seted up to the upper surface of mixing box, the inside of first through-hole is provided with first bearing, the inside of first bearing is provided with first axostylus axostyle, the surface of first axostylus axostyle is provided with first gear, the surface meshing of first gear has the second gear, the inside of second gear is provided with the second axostylus axostyle, the surface of second axostylus axostyle is provided with the second bearing, the second through-hole has been seted up to the inside of mixing box, the second bearing sets up the inside at the second through-hole, the surface of first axostylus axostyle and second axostyle all is provided with the puddler, the upper surface of mixing box is provided with the protection casing, the inner wall of protection casing is provided with first motor, the one end of first motor output shaft sets up on first axostylus axostyle, first axostyle and second axostyle all are located the inside of mixing box.
Optionally, the upper surface of base is provided with the support bottom ring, the upper surface of support bottom ring is provided with the guide arm, the upper end of guide arm is provided with the top ring.
Optionally, the upper surface of base sets up first protective housing, the inside of first protective housing is provided with the second motor, the surface of second motor output shaft sets up the third gear, the surface meshing of third gear has the outer tooth ring, the inside of outer tooth ring is provided with the centrifugal box, the outside of centrifugal box is provided with first spacing ring, the inside of supporting the bottom ring is provided with the connecting plate, the inside rotation of connecting plate is connected with the gyro wheel, gyro wheel and the mutual butt of first spacing ring, the outer tooth ring is located the inside of supporting the bottom ring.
Optionally, the inside of top ring is provided with the fixed plate, the inside rotation of fixed plate is connected with the pulley, the surface butt of pulley has the second spacing ring, the second spacing ring is located the upper end of centrifugal case.
Optionally, the upper surface of supporting the bottom ring is provided with the third protective housing, the inside of third protective housing is provided with the fourth motor, the surface of fourth motor output shaft is provided with the fourth gear, the surface meshing of fourth gear has the fifth gear, the inside of fifth gear is provided with the screw rod, the surface transmission of screw rod is connected with the heating ring, the inside of heating ring is provided with the heating wire, guide arm sliding connection is in the inside of heating ring, the surface of heating ring is provided with pressure sensor, the inner wall of heating ring is provided with temperature sensor.
Optionally, the upper surface of base is provided with the extraction section of thick bamboo, the inside sliding connection of extraction section of thick bamboo has the piston piece, the upper end of piston piece is provided with the connecting rod, the upper end of connecting rod is provided with the butt board, the upper surface of piston piece is provided with first spring, the one end setting of first spring is on the inner wall of extraction section of thick bamboo.
Optionally, the upper surface of extraction section of thick bamboo is provided with the second protective housing, the inside of second protective housing is provided with the third motor, the surface of third motor output shaft is provided with the eccentric wheel, the mutual butt of eccentric wheel and butt board.
Optionally, the lower extreme of mixing box is provided with electric valve, electric valve's one end is provided with first connecting pipe, the one end of first connecting pipe sets up in the extraction section of thick bamboo, the surface of extraction section of thick bamboo is provided with the second connecting pipe, the upper surface of base is provided with the support frame, the second connecting pipe sets up on the support frame, the one end of second connecting pipe is provided with first rotary joint, first rotary joint's setting is in the inside of centrifugal box, the lower extreme of centrifugal box is provided with second rotary joint, second rotary joint's lower extreme is provided with the fluid-discharge tube, second rotary joint is located the inside of base, the inside of fluid-discharge tube is provided with control valve, the lower surface of base is provided with the supporting leg, the inside of first connecting pipe and second connecting pipe is provided with the check valve.
(III) beneficial effects
The utility model provides a nucleic acid extraction and purification device, which has the following beneficial effects:
1. this nucleic acid extraction purification device, through the cooperation setting of first motor, first gear, the second gear, first axostylus axostyle, second axostylus axostyle and puddler, in the in-process that uses, add corresponding material and liquid into the mixing box, then through the rotation of first motor, drive first axostylus axostyle and first gear rotation, and then drive the second gear rotation, make the second axostylus axostyle rotate, make two puddles carry out the rotation of different rotational speeds simultaneously, form the turbulence, make corresponding material and liquid carry out intensive mixing, accelerate protein precipitation rate, thereby play the effect that improves mixing efficiency, the mesh that has reached the purification efficiency of improvement.
2. This nucleic acid extraction purification device, through the cooperation setting of heating ring, screw rod and fourth motor, in the in-process of using, through the rotation of fourth motor, drive fourth gear rotation, drive fifth gear rotation, drive the screw rod and rotate, and then drive the heating ring and rotate, make the heating ring slide along the guide bar, through the operation of heating wire, the heating centrifugal box, when the last pressure sensor of heating ring contacts top ring or third protective housing, send the signal of telecommunication, control fourth motor reversal, make the heating ring reciprocal slip in the surface of centrifugal box, evenly heat the centrifugal box, improve the extraction purity of nucleic acid.
Drawings
FIG. 1 is a schematic diagram of a front view of the present utility model;
FIG. 2 is a schematic view of the internal structure of the mixing box of the present utility model;
FIG. 3 is a schematic view of the internal structure of the protective cover of the present utility model;
FIG. 4 is a schematic view of the internal structure of the support bottom ring of the present utility model;
FIG. 5 is a schematic view of the internal structure of the extraction cartridge of the present utility model;
fig. 6 is a schematic view of a three-dimensional structure of the present utility model.
In the figure: 1. a base; 2. a mixing box; 3. a first shaft; 4. a first gear; 5. a second gear; 6. a second shaft; 7. a stirring rod; 8. a first motor; 9. a protective cover; 10. a support bottom ring; 11. a guide rod; 12. a top ring; 13. a first protective box; 14. a second motor; 15. an outer toothed ring; 16. a centrifugal box; 17. a first stop collar; 18. a pulley; 19. a third protective box; 20. a screw; 21. a heating ring; 22. a pressure sensor; 23. a drawing cylinder; 24. a piston block; 25. an abutting plate; 26. a first spring; 27. a second protective box; 28. an eccentric wheel; 29. a first connection pipe; 30. a first rotary joint; 31. and a liquid discharge pipe.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
Example 1
Referring to fig. 1 to 3, the present utility model provides the following technical solutions: a nucleic acid extraction purification device, comprising a base 1, characterized in that: the upper surface of base 1 is provided with mixing box 2, the first through-hole has been seted up to mixing box 2's upper surface, the inside of first through-hole is provided with first bearing, the inside of first bearing is provided with first axostylus axostyle 3, the surface of first axostylus axostyle 3 is provided with first gear 4, the surface meshing of first gear 4 has second gear 5, the inside of second gear 5 is provided with second axostylus axostyle 6, the surface of second axostylus axostyle 6 is provided with the second bearing, the inside of mixing box 2 has been seted up the second through-hole, the second bearing sets up the inside at the second through-hole, the surface of first axostylus axostyle 3 and second axostyle 6 all is provided with puddler 7, mixing box 2's upper surface is provided with protection casing 9, the inner wall of protection casing 9 is provided with first motor 8, the one end of first motor 8 output shaft sets up on first axostylus axostyle 3, first axostylus axostyle 3 and second axostyle 6 all are located mixing box 2 inside.
During the use, add corresponding material and liquid in the mixing box 2 through the feed inlet (the upper end of mixing box 2 is provided with the feed inlet), then through the rotation of first motor 8, drive first axostylus axostyle 3 and first gear 4 and rotate, and then drive second gear 5 and rotate, make second axostylus axostyle 6 rotate, make two puddler 7 carry out the rotation of different rotational speeds simultaneously, form the turbulent flow, make corresponding material and liquid carry out intensive mixing, accelerate the protein precipitation rate, thereby play the effect that improves mixing efficiency, the purpose that has reached the purification efficiency of improving.
Example 2
Referring to fig. 4 to 6, the present utility model provides the following technical solutions: the nucleic acid extraction purification device comprises a base 1, a supporting bottom ring 10 arranged on the upper surface of the base 1, a guide rod 11 arranged on the upper surface of the supporting bottom ring 10, a top ring 12 arranged at the upper end of the guide rod 11, a first protective box 13 arranged on the upper surface of the base 1, a second motor 14 arranged inside the first protective box 13, a third gear arranged on the outer surface of an output shaft of the second motor 14, an external tooth ring 15 meshed with the outer surface of the third gear, a centrifugal box 16 arranged inside the external tooth ring 15, a first limiting ring 17 arranged outside the centrifugal box 16, a connecting plate arranged inside the supporting bottom ring 10, a roller connected with the inner rotation of the connecting plate, the roller and the first limiting ring 17 in contact with each other, the external tooth ring 15 arranged inside the supporting bottom ring 10, a fixed plate arranged inside the top ring 12, a pulley 18 connected with the inner rotation of the fixed plate, and a second limiting ring connected with the outer surface of the pulley 18 in contact with the outer surface of the pulley 18, the second limiting ring is positioned at the upper end of the centrifugal box 16, the upper surface of the supporting bottom ring 10 is provided with a third protective box 19, the inside of the third protective box 19 is provided with a fourth motor, the outer surface of an output shaft of the fourth motor is provided with a fourth gear, the outer surface of the fourth gear is meshed with a fifth gear, the inside of the fifth gear is provided with a screw rod 20, the outer surface of the screw rod 20 is in transmission connection with a heating ring 21, the inside of the heating ring 21 is provided with an electric heating wire, a guide rod 11 is in sliding connection with the inside of the heating ring 21, the outer surface of the heating ring 21 is provided with a pressure sensor 22, the inner wall of the heating ring 21 is provided with a temperature sensor, the upper surface of the base 1 is provided with an extraction cylinder 23, the inside of the extraction cylinder 23 is in sliding connection with a piston block 24, the upper end of the piston block 24 is provided with a connecting rod, the upper end of the connecting rod is provided with a butt plate 25, the upper surface of the piston block 24 is provided with a first spring 26, one end of a first spring 26 is arranged on the inner wall of the extraction cylinder 23, the upper surface of the extraction cylinder 23 is provided with a second protective box 27, the inside of the second protective box 27 is provided with a third motor, the outer surface of an output shaft of the third motor is provided with an eccentric wheel 28, the eccentric wheel 28 is mutually abutted with the abutting plate 25, the lower end of the mixing box 2 is provided with an electric valve, one end of the electric valve is provided with a first connecting pipe 29, one end of the first connecting pipe 29 is arranged in the extraction cylinder 23, the outer surface of the extraction cylinder 23 is provided with a second connecting pipe, the upper surface of the base 1 is provided with a supporting frame, the second connecting pipe is arranged on the supporting frame, one end of the second connecting pipe is provided with a first rotary joint 30, the first rotary joint 30 is arranged inside the centrifugal box 16, the lower end of the centrifugal box 16 is provided with a second rotary joint, the lower end of the second rotary joint is provided with a liquid discharge pipe 31, the second rotary joint is positioned inside the base 1, the inside of the liquid discharge pipe 31 is provided with a control valve, the lower surface of the base 1 is provided with a supporting leg, and the inside of the first connecting pipe 29 and the second connecting pipe is provided with a one-way valve.
When in use, the electric valve is opened, and simultaneously, the eccentric wheel 28 is driven to rotate through the rotation of the third motor, so that the eccentric wheel 28 extrudes the abutting plate 25, the piston moves downwards, the first spring 26 is stretched, when the eccentric wheel 28 rotates to the upper part, the piston moves upwards under the elastic force of the first spring 26, the upper surface of the pressure extraction box in the extraction box is further changed, the air holes are formed in the upper surface of the pressure extraction box, the mixed extracted nucleic acid liquid is extracted into the centrifugal box 16 through the first connecting pipe 29 and the second connecting ring pipe, then the third gear is driven to rotate through the rotation of the second motor 14, the centrifugal box 16 is driven to rotate under the action of the external tooth ring 15, centrifugal purification is completed, and when the centrifugal box 16 rotates, through the rotation of the fourth motor, the fourth gear is driven to rotate, the fifth gear is driven to rotate, the screw rod 20 is driven to rotate, the heating ring 21 slides along the guiding rod, the centrifugal box 16 is heated through the operation of the heating wire (a power supply is arranged inside the heating box), when the upper pressure sensor 22 of the heating ring 21 contacts the top ring 12 or the third protective box 19, an electric signal is sent out, the fourth motor is controlled to rotate reversely, the heating ring 21 slides back and forth on the surface of the centrifugal box 16, the centrifugal box 16 is uniformly heated, the extraction purity of nucleic acid is improved (when the temperature sensor senses that the temperature of the surface of the centrifugal box 16 reaches a corresponding value, the electric signal is sent out, the operation of the fourth motor and the heating wire is stopped, and when the temperature is reduced, the centrifugal box is opened again).
The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.
Claims (8)
1. A nucleic acid extraction purification device, comprising a base (1), characterized in that: the upper surface of base (1) is provided with mixing box (2), the first through-hole has been seted up to the upper surface of mixing box (2), the inside of first through-hole is provided with first bearing, the inside of first bearing is provided with first axostylus axostyle (3), the surface of first axostylus axostyle (3) is provided with first gear (4), the surface meshing of first gear (4) has second gear (5), the inside of second gear (5) is provided with second axostylus axostyle (6), the surface of second axostylus axostyle (6) is provided with the second bearing, the second through-hole has been seted up to the inside of mixing box (2), the surface of first axostylus axostyle (3) and second axostylus axostyle (6) all is provided with puddler (7), the upper surface of mixing box (2) is provided with protection casing (9), the inner wall of protection casing (9) is provided with first motor (8), the one end setting of first motor (8) output shaft is on first (3), first axostylus axostyle (3) and second axostyle (6) are located inside of mixing box (2).
2. The nucleic acid extraction and purification apparatus according to claim 1, wherein: the novel support base is characterized in that a support bottom ring (10) is arranged on the upper surface of the base (1), a guide rod (11) is arranged on the upper surface of the support bottom ring (10), and a top ring (12) is arranged at the upper end of the guide rod (11).
3. The nucleic acid extraction and purification apparatus according to claim 2, wherein: the upper surface of base (1) sets up first protective housing (13), the inside of first protective housing (13) is provided with second motor (14), the surface of second motor (14) output shaft sets up the third gear, the surface meshing of third gear has outer ring gear (15), the inside of outer ring gear (15) is provided with centrifugal box (16), the outside of centrifugal box (16) is provided with first spacing ring (17), the inside of supporting bottom ring (10) is provided with the connecting plate, the inside rotation of connecting plate is connected with the gyro wheel, gyro wheel and mutual butt of first spacing ring (17), outer ring gear (15) are located the inside of supporting bottom ring (10).
4. The nucleic acid extraction and purification apparatus according to claim 2, wherein: the inside of top ring (12) is provided with the fixed plate, the inside rotation of fixed plate is connected with pulley (18), the surface butt of pulley (18) has the second spacing ring, the second spacing ring is located the upper end of centrifugal box (16).
5. The nucleic acid extraction and purification apparatus according to claim 2, wherein: the upper surface of support base ring (10) is provided with third protective housing (19), the inside of third protective housing (19) is provided with the fourth motor, and the surface of fourth motor output shaft is provided with the fourth gear, the surface meshing of fourth gear has the fifth gear, the inside of fifth gear is provided with screw rod (20), the surface transmission of screw rod (20) is connected with heating ring (21), the inside of heating ring (21) is provided with the heating wire, guide arm (11) sliding connection is in the inside of heating ring (21), the surface of heating ring (21) is provided with pressure sensor (22), the inner wall of heating ring (21) is provided with temperature-sensing ware.
6. The nucleic acid extraction and purification apparatus according to claim 1, wherein: the novel oil extraction device is characterized in that an extraction cylinder (23) is arranged on the upper surface of the base (1), a piston block (24) is connected to the inside of the extraction cylinder (23) in a sliding mode, a connecting rod is arranged at the upper end of the piston block (24), an abutting plate (25) is arranged at the upper end of the connecting rod, a first spring (26) is arranged on the upper surface of the piston block (24), and one end of the first spring (26) is arranged on the inner wall of the extraction cylinder (23).
7. The nucleic acid extraction and purification apparatus according to claim 6, wherein: the upper surface of extraction section of thick bamboo (23) is provided with second protective housing (27), the inside of second protective housing (27) is provided with the third motor, and the surface of third motor output shaft is provided with eccentric wheel (28), eccentric wheel (28) and butt board (25) butt each other.
8. The nucleic acid extraction and purification apparatus according to claim 1, wherein: the lower extreme of mixing box (2) is provided with electric valve, electric valve's one end is provided with first connecting pipe (29), the one end of first connecting pipe (29) sets up in extraction section of thick bamboo (23), the surface of extraction section of thick bamboo (23) is provided with the second connecting pipe, the upper surface of base (1) is provided with the support frame, the second connecting pipe sets up on the support frame, the one end of second connecting pipe is provided with first rotary joint (30), the inside at centrifugal box (16) of setting of first rotary joint (30), the lower extreme of centrifugal box (16) is provided with second rotary joint, the lower extreme of second rotary joint is provided with fluid-discharge tube (31), second rotary joint is located the inside of base (1), the inside of fluid-discharge tube (31) is provided with control valve, the lower surface of base (1) is provided with the supporting leg, the inside of first connecting pipe (29) and second connecting pipe is provided with the check valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221974323.7U CN219314902U (en) | 2022-07-26 | 2022-07-26 | Nucleic acid extraction and purification device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221974323.7U CN219314902U (en) | 2022-07-26 | 2022-07-26 | Nucleic acid extraction and purification device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219314902U true CN219314902U (en) | 2023-07-07 |
Family
ID=87020792
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221974323.7U Active CN219314902U (en) | 2022-07-26 | 2022-07-26 | Nucleic acid extraction and purification device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219314902U (en) |
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2022
- 2022-07-26 CN CN202221974323.7U patent/CN219314902U/en active Active
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